Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38895.t1 | XP_013445348.1 | 81.8 | 686 | 106 | 4 | 2 | 682 | 114 | 785 | 0.00E+00 | 1147.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38895.t1 | Q9LW83 | 40.5 | 719 | 341 | 19 | 8 | 649 | 111 | 819 | 4.3e-132 | 473.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38895.t1 | A0A072TPH7 | 81.8 | 686 | 106 | 4 | 2 | 682 | 114 | 785 | 0.0e+00 | 1147.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049404 | MS.gene38895 | 0.801815 | 7.53E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene38895 | 0.808387 | 3.18E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene38895 | 0.820429 | 6.89E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene38895 | 0.812888 | 3.38E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene38895 | 0.803804 | 2.93E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene38895 | 0.815929 | 7.19E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene38895 | 0.809516 | 1.82E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene38895 | 0.808686 | 2.74E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene38895 | 0.810835 | 9.46E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene38895 | 0.809001 | 2.35E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene38895 | 0.824809 | 6.60E-54 | -1.69E-46 |
| MS.gene058625 | MS.gene38895 | 0.814777 | 1.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 126 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGGATTTCTACCTATT+TGG | 0.182797 | 8.4:+50971369 | MS.gene38895:CDS |
| GACGAAGAGTAAATGATTAT+TGG | 0.211979 | 8.4:-50956160 | None:intergenic |
| CGTTGGGATCAAAACATCTA+TGG | 0.254981 | 8.4:-50956293 | None:intergenic |
| AATATACTCTTTGTCCCATT+TGG | 0.254995 | 8.4:-50956250 | None:intergenic |
| TGGCTTTACCAAGGTGTAAA+AGG | 0.288350 | 8.4:-50956953 | None:intergenic |
| ACATAGAATGCTGGTCTTCT+TGG | 0.291640 | 8.4:-50972317 | None:intergenic |
| ATCAATCTTGGAAGCAACTA+TGG | 0.296040 | 8.4:+50970714 | MS.gene38895:CDS |
| ATGTTCAATTCACCTTGTTT+TGG | 0.297418 | 8.4:-50956418 | None:intergenic |
| ACTCCTTTGCTTATAAATTC+AGG | 0.301314 | 8.4:+50956373 | MS.gene38895:CDS |
| TGGAATATTCGAGGACATTC+CGG | 0.301663 | 8.4:+50956489 | MS.gene38895:CDS |
| AGTCCTTCCATGGCATATTC+TGG | 0.305495 | 8.4:-50971913 | None:intergenic |
| TGGTGAGGTGTTTCAGAAAA+AGG | 0.325225 | 8.4:+50956744 | MS.gene38895:CDS |
| GATCGTCCACTAAATCTAAT+AGG | 0.344872 | 8.4:+50972024 | MS.gene38895:CDS |
| TCTACCTCATGTGAAGTAGA+AGG | 0.352480 | 8.4:+50972413 | MS.gene38895:CDS |
| AGGCTATGGACCAGTTTATA+AGG | 0.356418 | 8.4:+50970768 | MS.gene38895:CDS |
| TCAACATATTCTCACCAAAT+AGG | 0.361054 | 8.4:-50971383 | None:intergenic |
| TAGTCAATGCAGGTATAAAA+AGG | 0.363780 | 8.4:+50970405 | MS.gene38895:intron |
| ACAAAATGTTGAAGTGGTTT+CGG | 0.368706 | 8.4:+50956927 | MS.gene38895:CDS |
| GAATTCCACGATTCCTTGTC+CGG | 0.373228 | 8.4:-50971232 | None:intergenic |
| ATATACTCTTTGTCCCATTT+GGG | 0.378379 | 8.4:-50956249 | None:intergenic |
| ACATGTCCTATTAGATTTAG+TGG | 0.382386 | 8.4:-50972030 | None:intergenic |
| CTTCAGGGAATCTTGGCAAC+AGG | 0.383491 | 8.4:+50971174 | MS.gene38895:intron |
| CAGGTGAGTTTAGTCTTGAA+TGG | 0.385800 | 8.4:+50956392 | MS.gene38895:CDS |
| TTCACTTTCAAAGTTAAAGA+TGG | 0.388071 | 8.4:+50956568 | MS.gene38895:CDS |
| TTTGCAGTCACCTTATAAAC+TGG | 0.391170 | 8.4:-50970778 | None:intergenic |
| AACCGGACATAATTGGTCAT+TGG | 0.393190 | 8.4:+50956339 | MS.gene38895:CDS |
| ATGGGACAAAGAGTATATTA+TGG | 0.393795 | 8.4:+50956254 | MS.gene38895:CDS |
| AACACACATCCAGTCCTATT+AGG | 0.402538 | 8.4:-50956886 | None:intergenic |
| TTTACGACTTCAGGGAATCT+TGG | 0.406797 | 8.4:+50971167 | MS.gene38895:intron |
| CTTCAACAACTTCACCCAAA+TGG | 0.407409 | 8.4:+50956235 | MS.gene38895:CDS |
| AAGCGTTTCAACATAATAGA+AGG | 0.410503 | 8.4:+50971600 | MS.gene38895:CDS |
| GGGAGGATATACCAACTTGC+AGG | 0.423249 | 8.4:+50956716 | MS.gene38895:CDS |
| ACTGAGGAGGGGAGGTTAGT+AGG | 0.434939 | 8.4:+50956613 | MS.gene38895:CDS |
| GGTGTCAGTGTCTTTCCCTT+TGG | 0.439410 | 8.4:-50972372 | None:intergenic |
| TTGATCCCAACGATGAAGTT+GGG | 0.440820 | 8.4:+50956304 | MS.gene38895:CDS |
| GGAAGGTAAGAGCAATGATT+TGG | 0.441227 | 8.4:+50970621 | MS.gene38895:CDS |
| CTGGATGTGTGTTCTATTCA+TGG | 0.444234 | 8.4:+50956896 | MS.gene38895:CDS |
| AGAAATTGTTGTCGTAGAAT+AGG | 0.445993 | 8.4:-50972393 | None:intergenic |
| AGAAAAGTGGCAAAGTGTAT+TGG | 0.447357 | 8.4:+50956443 | MS.gene38895:CDS |
| GGCAAAGTGTATTGGAAAAG+TGG | 0.448233 | 8.4:+50956451 | MS.gene38895:CDS |
| CTAACCTCCCCTCCTCAGTT+AGG | 0.454952 | 8.4:-50956609 | None:intergenic |
| AAGTCTGACGTCTATAGCTT+TGG | 0.456291 | 8.4:+50971946 | MS.gene38895:CDS |
| TGCTTCCAAGATTGATGTAA+AGG | 0.458700 | 8.4:-50970707 | None:intergenic |
| GTAAATGCAGATATGTGTTA+TGG | 0.459089 | 8.4:+50956664 | MS.gene38895:CDS |
| GTTGTTGAATTGTCTATGCT+TGG | 0.460230 | 8.4:-50956775 | None:intergenic |
| TGGTGAGTATCTAAAGCTAA+TGG | 0.461240 | 8.4:+50972145 | MS.gene38895:CDS |
| AGGTGAGTTTAGTCTTGAAT+GGG | 0.461259 | 8.4:+50956393 | MS.gene38895:CDS |
| TAACTCCCAACTTCATCGTT+GGG | 0.461807 | 8.4:-50956309 | None:intergenic |
| AGAAATCTTGTCCAACTACT+TGG | 0.463391 | 8.4:+50971291 | MS.gene38895:CDS |
| TTCAACAACTTCACCCAAAT+GGG | 0.463790 | 8.4:+50956236 | MS.gene38895:CDS |
| GTTGCATTCATGAAGAAGAA+AGG | 0.470569 | 8.4:+50971316 | MS.gene38895:CDS |
| GGGAGGTTAGTAGGTGGTGA+TGG | 0.474740 | 8.4:+50956622 | MS.gene38895:CDS |
| AAGTTAGGACAGGGAGGCTA+TGG | 0.474937 | 8.4:+50970754 | MS.gene38895:CDS |
| AGATCTTGAAGATGATTTCA+AGG | 0.477563 | 8.4:+50970663 | MS.gene38895:CDS |
| TTGCTACTTGAAATCGTTTG+TGG | 0.485028 | 8.4:+50971973 | MS.gene38895:CDS |
| GTTGGAAGAGAGATATTTGA+GGG | 0.486425 | 8.4:+50972338 | MS.gene38895:CDS |
| TAAATACCAACAGAATTGTT+GGG | 0.486818 | 8.4:+50971778 | MS.gene38895:CDS |
| CCCCGAAAACAAGTTAGGAC+AGG | 0.487573 | 8.4:+50970744 | MS.gene38895:CDS |
| AATTCTGTTGGTATTTACTA+CGG | 0.487780 | 8.4:-50971772 | None:intergenic |
| TTTGATCCCAACGATGAAGT+TGG | 0.492176 | 8.4:+50956303 | MS.gene38895:CDS |
| ACAAATGCGTCATTTAATGT+TGG | 0.493128 | 8.4:-50972170 | None:intergenic |
| GAATGCTGGTCTTCTTGGTA+AGG | 0.494868 | 8.4:-50972312 | None:intergenic |
| AACCAATGACCAATTATGTC+CGG | 0.495276 | 8.4:-50956341 | None:intergenic |
| ACTTACGTACCATAGGAATT+CGG | 0.496040 | 8.4:-50956988 | None:intergenic |
| GTAAATACCAACAGAATTGT+TGG | 0.499094 | 8.4:+50971777 | MS.gene38895:CDS |
| GGCAAGAATGTTTACACAGC+AGG | 0.500524 | 8.4:+50971746 | MS.gene38895:CDS |
| GATATAACATCAGACATTGT+TGG | 0.501777 | 8.4:-50972260 | None:intergenic |
| ATTAATAGGCATGGGAGCTA+TGG | 0.510326 | 8.4:+50972117 | MS.gene38895:intron |
| ATATATGCCAAACAAAAGCT+TGG | 0.511119 | 8.4:+50971353 | MS.gene38895:CDS |
| TTAACTCCCAACTTCATCGT+TGG | 0.515739 | 8.4:-50956310 | None:intergenic |
| AAATCAGCTCCGAATTCCTA+TGG | 0.518779 | 8.4:+50956979 | MS.gene38895:CDS |
| GAATGCAACAGCCAAGTAGT+TGG | 0.519428 | 8.4:-50971302 | None:intergenic |
| CAACACTGTCGCCACTATGT+TGG | 0.522256 | 8.4:+50956195 | MS.gene38895:CDS |
| AGTGGAAAACTTAAAAGCAA+TGG | 0.524344 | 8.4:+50956469 | MS.gene38895:CDS |
| TACATATGTTGAACATTCGC+CGG | 0.532156 | 8.4:-50956508 | None:intergenic |
| GATCTTGAAGATGATTTCAA+GGG | 0.533388 | 8.4:+50970664 | MS.gene38895:CDS |
| CATAGTGGCGACAGTGTTGT+TGG | 0.534940 | 8.4:-50956191 | None:intergenic |
| TTCGACACAAAATGTTGAAG+TGG | 0.537451 | 8.4:+50956921 | MS.gene38895:CDS |
| TGTTGGAAGAGAGATATTTG+AGG | 0.545434 | 8.4:+50972337 | MS.gene38895:CDS |
| CTACCTCATGTGAAGTAGAA+GGG | 0.547478 | 8.4:+50972414 | MS.gene38895:CDS |
| TAAAAGCAATGGAATATTCG+AGG | 0.550559 | 8.4:+50956480 | MS.gene38895:CDS |
| TCTCTTCCAACATAGAATGC+TGG | 0.550948 | 8.4:-50972326 | None:intergenic |
| TGGCTAGGCATAAAATGAGC+GGG | 0.551974 | 8.4:-50970475 | None:intergenic |
| AGGTAAGAGCAATGATTTGG+TGG | 0.556298 | 8.4:+50970624 | MS.gene38895:CDS |
| CGATGGTACCTAACTGAGGA+GGG | 0.557648 | 8.4:+50956601 | MS.gene38895:CDS |
| GTTACCAGTGTCCAACATAG+TGG | 0.560687 | 8.4:-50956206 | None:intergenic |
| TCACCTGAATTTATAAGCAA+AGG | 0.560899 | 8.4:-50956376 | None:intergenic |
| TCTATCTTCATAAGTACTCA+AGG | 0.570041 | 8.4:+50971640 | MS.gene38895:CDS |
| TGTAGAACAAAGTCCTTCCA+TGG | 0.571345 | 8.4:-50971923 | None:intergenic |
| GTTGGGATCAAAACATCTAT+GGG | 0.575637 | 8.4:-50956292 | None:intergenic |
| AGAAGACCAGCATTCTATGT+TGG | 0.575785 | 8.4:+50972320 | MS.gene38895:CDS |
| GTGATTGCAAGATGAGATGT+TGG | 0.577780 | 8.4:+50956827 | MS.gene38895:CDS |
| ATACCAACTTGCAGGGACCC+TGG | 0.583981 | 8.4:+50956724 | MS.gene38895:CDS |
| CTATGTCCCAACAATTCTGT+TGG | 0.584311 | 8.4:-50971784 | None:intergenic |
| GAATTGAACATCAAGAAAAG+TGG | 0.585092 | 8.4:+50956430 | MS.gene38895:CDS |
| TTCAGGGAATCTTGGCAACA+GGG | 0.588092 | 8.4:+50971175 | MS.gene38895:intron |
| ATAATAGAAGGAATTGCTCA+AGG | 0.593195 | 8.4:+50971612 | MS.gene38895:CDS |
| GCATGGGAGCTATGGAATGA+TGG | 0.593901 | 8.4:+50972125 | MS.gene38895:CDS |
| CTTGAATGGGAACCAAAACA+AGG | 0.593990 | 8.4:+50956406 | MS.gene38895:CDS |
| CCCGAAAACAAGTTAGGACA+GGG | 0.595234 | 8.4:+50970745 | MS.gene38895:CDS |
| TGGAAGAGAGATATTTGAGG+GGG | 0.595624 | 8.4:+50972340 | MS.gene38895:CDS |
| GTGGCGACAGTGTTGTTGGT+GGG | 0.604500 | 8.4:-50956187 | None:intergenic |
| CAATTCAACAACGTATGAAG+AGG | 0.604602 | 8.4:+50956786 | MS.gene38895:CDS |
| GTCGCCACTATGTTGGACAC+TGG | 0.607349 | 8.4:+50956202 | MS.gene38895:CDS |
| AAGATGGAAAGTTTATGCGA+TGG | 0.607356 | 8.4:+50956584 | MS.gene38895:CDS |
| TCTCCAGAATATGCCATGGA+AGG | 0.609953 | 8.4:+50971910 | MS.gene38895:CDS |
| TGTTATGGATATAATAGTGA+TGG | 0.613097 | 8.4:+50956679 | MS.gene38895:CDS |
| TCACCAGGGTCCCTGCAAGT+TGG | 0.616521 | 8.4:-50956727 | None:intergenic |
| AACATCCGGACAAGGAATCG+TGG | 0.616760 | 8.4:+50971227 | MS.gene38895:CDS |
| TATGGATATAATAGTGATGG+AGG | 0.618660 | 8.4:+50956682 | MS.gene38895:CDS |
| TTTCCCTTCTACTTCACATG+AGG | 0.619457 | 8.4:-50972417 | None:intergenic |
| CATGTCTCCAGAATATGCCA+TGG | 0.619976 | 8.4:+50971906 | MS.gene38895:CDS |
| AGTGGCGACAGTGTTGTTGG+TGG | 0.620468 | 8.4:-50956188 | None:intergenic |
| GGAAGAGAGATATTTGAGGG+GGG | 0.624121 | 8.4:+50972341 | MS.gene38895:CDS |
| TTGGCTAGGCATAAAATGAG+CGG | 0.632029 | 8.4:-50970476 | None:intergenic |
| TTGTTCTTGATGAAGTGCAA+AGG | 0.633912 | 8.4:+50972189 | MS.gene38895:CDS |
| GCGATGGTACCTAACTGAGG+AGG | 0.636389 | 8.4:+50956600 | MS.gene38895:CDS |
| GGAGGATATACCAACTTGCA+GGG | 0.636612 | 8.4:+50956717 | MS.gene38895:CDS |
| TTGGAAGAGAGATATTTGAG+GGG | 0.643866 | 8.4:+50972339 | MS.gene38895:CDS |
| AACTTGCAGGGACCCTGGTG+AGG | 0.662301 | 8.4:+50956729 | MS.gene38895:CDS |
| GAGGAGGGGAGGTTAGTAGG+TGG | 0.665236 | 8.4:+50956616 | MS.gene38895:CDS |
| GGTACCTAACTGAGGAGGGG+AGG | 0.665972 | 8.4:+50956605 | MS.gene38895:CDS |
| GATGGTACCTAACTGAGGAG+GGG | 0.676323 | 8.4:+50956602 | MS.gene38895:CDS |
| GTGCAAAGGTGCATTCATGT+TGG | 0.682981 | 8.4:+50972203 | MS.gene38895:CDS |
| TATGCGATGGTACCTAACTG+AGG | 0.725081 | 8.4:+50956597 | MS.gene38895:CDS |
| GAAAACAAGTTAGGACAGGG+AGG | 0.747415 | 8.4:+50970748 | MS.gene38895:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 50956153 | 50972445 | 50956153 | ID=MS.gene38895 |
| chr8.4 | mRNA | 50956153 | 50972445 | 50956153 | ID=MS.gene38895.t1;Parent=MS.gene38895 |
| chr8.4 | exon | 50956153 | 50957000 | 50956153 | ID=MS.gene38895.t1.exon1;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50956153 | 50957000 | 50956153 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| chr8.4 | exon | 50970417 | 50970509 | 50970417 | ID=MS.gene38895.t1.exon2;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50970417 | 50970509 | 50970417 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| chr8.4 | exon | 50970602 | 50970789 | 50970602 | ID=MS.gene38895.t1.exon3;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50970602 | 50970789 | 50970602 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| chr8.4 | exon | 50971180 | 50971390 | 50971180 | ID=MS.gene38895.t1.exon4;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50971180 | 50971390 | 50971180 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| chr8.4 | exon | 50971568 | 50971805 | 50971568 | ID=MS.gene38895.t1.exon5;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50971568 | 50971805 | 50971568 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| chr8.4 | exon | 50971900 | 50972050 | 50971900 | ID=MS.gene38895.t1.exon6;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50971900 | 50972050 | 50971900 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| chr8.4 | exon | 50972125 | 50972445 | 50972125 | ID=MS.gene38895.t1.exon7;Parent=MS.gene38895.t1 |
| chr8.4 | CDS | 50972125 | 50972445 | 50972125 | ID=cds.MS.gene38895.t1;Parent=MS.gene38895.t1 |
| Gene Sequence |
| Protein sequence |
