AlfalfaGEDB | MS.gene39536

M. sativa cultivar XinJiangDaYe / MS.gene39536

KEGG: K23179
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39536.t1	XP_013456927.1	92.5	522	39	0	1	522	1	522	1.10E-275	958.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39536.t1	Q9SXS3	81.9	524	92	2	1	522	1	523	1.5e-249	863.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39536.t1	A0A072UPL5	92.5	522	39	0	1	522	1	522	7.7e-276	958.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055124	MS.gene39536	0.806893	6.59E-50	-1.69E-46
MS.gene056218	MS.gene39536	0.801073	1.07E-48	-1.69E-46
MS.gene056310	MS.gene39536	0.802749	4.84E-49	-1.69E-46
MS.gene056313	MS.gene39536	0.821367	4.19E-53	-1.69E-46
MS.gene057291	MS.gene39536	0.801833	7.47E-49	-1.69E-46
MS.gene059039	MS.gene39536	0.806772	6.99E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39536.t1	MTR_4g088160	96.552	522	18	0	1	522	1	522	0.0	1045
MS.gene39536.t1	MTR_4g088170	91.969	523	40	1	1	521	1	523	0.0	1002
MS.gene39536.t1	MTR_4g088195	94.253	522	30	0	1	522	1	522	0.0	998
MS.gene39536.t1	MTR_7g027960	52.216	519	239	6	5	521	6	517	0.0	562
MS.gene39536.t1	MTR_7g028020	51.638	519	242	6	5	521	6	517	0.0	558
MS.gene39536.t1	MTR_4g062500	44.422	502	253	9	26	512	27	517	2.16e-155	454
MS.gene39536.t1	MTR_4g062510	43.271	483	249	8	46	512	48	521	1.33e-150	441
MS.gene39536.t1	MTR_7g092620	45.082	488	240	9	35	512	35	504	4.20e-147	432
MS.gene39536.t1	MTR_1g107295	42.857	483	248	8	46	512	49	519	2.80e-146	431
MS.gene39536.t1	MTR_0171s0020	41.566	498	264	7	24	512	26	505	2.13e-145	427

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39536.t1	AT5G06900	35.417	528	289	13	1	517	11	497	3.48e-110	337
MS.gene39536.t1	AT3G20110	34.959	492	277	8	32	513	40	498	1.84e-99	310
MS.gene39536.t1	AT4G15360	32.895	532	310	9	7	522	15	515	2.20e-99	310
MS.gene39536.t1	AT3G20940	34.406	497	293	8	26	512	36	509	9.70e-97	303
MS.gene39536.t1	AT4G15330	33.469	490	287	9	32	510	39	500	2.98e-96	301
MS.gene39536.t1	AT3G20950	35.542	498	289	8	26	512	36	512	3.97e-96	301
MS.gene39536.t1	AT4G15350	34.440	482	279	10	46	513	45	503	5.28e-96	301
MS.gene39536.t1	AT5G47990	35.294	476	278	7	46	512	53	507	7.22e-96	300
MS.gene39536.t1	AT1G50520	34.082	490	287	8	45	521	53	519	1.02e-95	301
MS.gene39536.t1	AT3G20140	33.871	496	286	10	35	517	43	509	5.02e-94	295

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Find 135 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATCAACGCTCCTTC+TGG	0.243222	4.2:-23178660	None:intergenic
AAACCTCGTCTTCCATTCAT+TGG	0.406823	4.2:+23177299	MS.gene39536:CDS
AAAGTATCAACGCTCCTTCT+GGG	0.412412	4.2:-23178659	None:intergenic
AACACCTACTGCAAAATCAA+AGG	0.600050	4.2:+23177244	MS.gene39536:CDS
AACTGCAAGTTCCACCAACA+TGG	0.609692	4.2:-23177183	None:intergenic
AACTTCCAATAAGGTCCAAA+TGG	0.454113	4.2:-23177551	None:intergenic
AAGACCCTTGATTTGTTCCT+TGG	0.396454	4.2:-23178053	None:intergenic
AAGCAGCTTCAATTGATCTT+AGG	0.233064	4.2:+23178775	MS.gene39536:CDS
AAGCTACTTCCTTTAACACA+AGG	0.615365	4.2:+23177474	MS.gene39536:CDS
AAGTAGCTTCATGGGTTTGA+AGG	0.436803	4.2:-23177460	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAAATGCAAGGTCTG+GGG	-	chr4.2:23178157-23178176	None:intergenic	30.0%
AAAAAATGGAGAAATCGTAG+AGG	+	chr4.2:23177958-23177977	MS.gene39536:CDS	30.0%
AAAAATGGAGAAATCGTAGA+GGG	+	chr4.2:23177959-23177978	MS.gene39536:CDS	30.0%
AATTACCAAGGAACAAATCA+AGG	+	chr4.2:23178048-23178067	MS.gene39536:CDS	30.0%
ATTACCAAGGAACAAATCAA+GGG	+	chr4.2:23178049-23178068	MS.gene39536:CDS	30.0%
ATTTCCAACTTCTACCATTT+GGG	+	chr4.2:23178805-23178824	MS.gene39536:CDS	30.0%
GAAAAAAAAAATGCAAGGTC+TGG	-	chr4.2:23178159-23178178	None:intergenic	30.0%
GTATGATCCTATCATTGAAA+AGG	+	chr4.2:23177901-23177920	MS.gene39536:CDS	30.0%
AACACCTACTGCAAAATCAA+AGG	+	chr4.2:23177244-23177263	MS.gene39536:CDS	35.0%
AACTTCCAATAAGGTCCAAA+TGG	-	chr4.2:23177554-23177573	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	23177185	23179067	23177185	ID=MS.gene39536
chr4.2	mRNA	23177185	23179067	23177185	ID=MS.gene39536.t1;Parent=MS.gene39536
chr4.2	exon	23177185	23178081	23177185	ID=MS.gene39536.t1.exon1;Parent=MS.gene39536.t1
chr4.2	CDS	23177185	23178081	23177185	ID=cds.MS.gene39536.t1;Parent=MS.gene39536.t1
chr4.2	exon	23178396	23179067	23178396	ID=MS.gene39536.t1.exon2;Parent=MS.gene39536.t1
chr4.2	CDS	23178396	23179067	23178396	ID=cds.MS.gene39536.t1;Parent=MS.gene39536.t1

Gene Sequence

>MS.gene39536

ATGTTGGTGGAACTTGCAGTTACTCTATTGCTCATTGCTCTCTTCTTACACTTGCGTCCAACACCTACTGCAAAATCAAAGGCTCTTCGCCACCTTCCCAATCCTCCGAGTCCCAAACCTCGTCTTCCATTCATTGGTCACCTTCACCTTTTGGATAACCCTCTTCTTCACCACACTCTTATCAAGTTAGGAGAACGTTATGGCCCTTTGTACACTCTTTACTTCGGTTCCATGCCTACCGTTGTTGCCTCCACTCCTGACTTGTTCAAACTTTTCCTTCAAACCCATGAAGCTACTTCCTTTAACACAAGGTTCCAAACCACTGCTATTAGTCGTCTTACCTATGACAACTCTGTTGCTATGGTTCCATTTGGACCTTATTGGAAGTTTATTAGAAAGCTTATCATGAACGACTTGCTCAACGCCACCACTGTTAACAAGTTAAGGCCATTGAGGAGCCGAGAAATCCGTAAGGTTCTTAAGGTTATGGCTAATAGTGCTGAAACTCAACAGCCACTTAATGTCACCGAGGAGCTTCTCAAGTGGACAAACAGCACAATCTCTACGATGATGTTGGGTGAGGCGGAAGAGGTTAGAGATATTGCTCGCGATGTTCTTAAGATCTTTGGAGAATATAGTGTTACAAACTTCATATGGCCATTGAACAAGTTTAAGTTTGGAAACTATGAGAAGAGAATTGACGAGATTTTCAATAAGTATGATCCTATCATTGAAAAGGTTATCAATAAGCGACAAGAGATTGTGAACAAAAGAAAAAATGGAGAAATCGTAGAGGGCGAGCAGAGTGTAGTTTTTCTTGATACTTTGCTTGAATATGCTCAGGATGAGACCATGGACATCAAAATTACCAAGGAACAAATCAAGGGTCTTGTTGTGGTAAGTTTCTTATAATGTCTTTTTTTTCTTATCTTCTTTTTTTTTTTTTTTTGGTGGTGGCCACGGTTTGAACCCCAGACCTTGCATTTTTTTTTTCTTATCTTCTAATTATATGCTAATTAATTTAAATTTCATCAATCAAGAGTATTAAGTATTTAATTGAGATGTTATTCGACTACGAAAGACTTTTAATTTTATACCACTGTGGCGCTTTTGAATATGGCTTAGTAAGTTATGTGTTTGTACTATGAAGCAAACTTGCAATTTTACGTCACTAGCAATGCTCTTACATTCACAACGCTTTGTGTGTGCAGGATTTCTTCTCTGCAGGAACAGATTCCACGGCTGTGTCAACTGAATGGACTTTATCAGAGCTCATCAACAATCCTAGAGTGTTGAAAAAAGCTCGAGAGGAGATTGACTCTATTGTGGGAAAAGATAGACTGGTTGATGAATCAGATGTTCAGAATCTTCCTTACATTAGAGCCATCGTGAAGGAAGCATTCCGCATGCACCCACCACTACCTGTAGTCAAGAGAAAATGTACACAAGAATGTGAGATCGACGGGTATGTGATCCCAGAAGGAGCGTTGATACTTTTTAATGTTTGGGCAGTGGGAAGAGACCCAAAATATTGGGAAAAACCATTAGAATTCCGACCTGAGAGGTTCATAGAAAATGTTGGTGAAGGTGAAGCAGCTTCAATTGATCTTAGGGGTCAGCATTTCCAACTTCTACCATTTGGGTCTGGAAGGAGAATGTGTCCTGGAGTCAATTTGGCTACTGCAGGAATGGCCACACTGCTTGCATCTATTATCCAGTGCTTTGATCTAGAGGTACCAGGCCCAAATGGGCAAATACTGAAAGGTATTGATGCTAAGGTTAGCATGGAAGAGAGACCTGGTCTCACAGTTCCAAGGGCACATGATCTCATGTGTGTTCCACTCGCAAGAGCCGGTGTCGCAGCTAAACTTCTCTCCTCCTAA

Protein sequence

>MS.gene39536.t1

MLVELAVTLLLIALFLHLRPTPTAKSKALRHLPNPPSPKPRLPFIGHLHLLDNPLLHHTLIKLGERYGPLYTLYFGSMPTVVASTPDLFKLFLQTHEATSFNTRFQTTAISRLTYDNSVAMVPFGPYWKFIRKLIMNDLLNATTVNKLRPLRSREIRKVLKVMANSAETQQPLNVTEELLKWTNSTISTMMLGEAEEVRDIARDVLKIFGEYSVTNFIWPLNKFKFGNYEKRIDEIFNKYDPIIEKVINKRQEIVNKRKNGEIVEGEQSVVFLDTLLEYAQDETMDIKITKEQIKGLVVDFFSAGTDSTAVSTEWTLSELINNPRVLKKAREEIDSIVGKDRLVDESDVQNLPYIRAIVKEAFRMHPPLPVVKRKCTQECEIDGYVIPEGALILFNVWAVGRDPKYWEKPLEFRPERFIENVGEGEAASIDLRGQHFQLLPFGSGRRMCPGVNLATAGMATLLASIIQCFDLEVPGPNGQILKGIDAKVSMEERPGLTVPRAHDLMCVPLARAGVAAKLLSS