Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059039.t1 | XP_003594831.1 | 94.9 | 158 | 8 | 0 | 1 | 158 | 1 | 158 | 6.00E-81 | 310.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059039.t1 | P27047 | 84.2 | 158 | 25 | 0 | 1 | 158 | 1 | 158 | 5.2e-73 | 275.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059039.t1 | G7IMY7 | 94.9 | 158 | 8 | 0 | 1 | 158 | 1 | 158 | 4.3e-81 | 310.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054138 | MS.gene059039 | 0.811392 | 7.16E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene059039 | 0.802369 | 5.80E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene059039 | 0.804413 | 2.19E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene059039 | 0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene065728 | 0.806239 | 9.06E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene065729 | 0.815534 | 8.80E-52 | -1.69E-46 |
| MS.gene059039 | MS.gene06593 | 0.809076 | 2.26E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene06805 | 0.808686 | 2.74E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene070142 | 0.80148 | 8.83E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene071840 | 0.900569 | 6.43E-78 | -1.69E-46 |
| MS.gene059039 | MS.gene07241 | 0.816864 | 4.44E-52 | -1.69E-46 |
| MS.gene059039 | MS.gene20126 | 0.802082 | 6.64E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene26289 | 0.801842 | 7.44E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene26404 | 0.803542 | 3.32E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene32342 | 0.80415 | 2.48E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene39536 | 0.806772 | 6.99E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene42005 | 0.816294 | 5.96E-52 | -1.69E-46 |
| MS.gene059039 | MS.gene47575 | 0.809761 | 1.61E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene67371 | 0.800722 | 1.26E-48 | -1.69E-46 |
| MS.gene059039 | MS.gene71342 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene71346 | 0.814201 | 1.74E-51 | -1.69E-46 |
| MS.gene059039 | MS.gene81770 | 0.813862 | 2.06E-51 | -1.69E-46 |
| MS.gene059039 | MS.gene85346 | 0.802776 | 4.78E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene91222 | 0.800899 | 1.16E-48 | -1.69E-46 |
| MS.gene059039 | MS.gene91226 | 0.805329 | 1.41E-49 | -1.69E-46 |
| MS.gene059039 | MS.gene91754 | 0.8003 | 1.54E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059039.t1 | MTR_2g035120 | 94.937 | 158 | 8 | 0 | 1 | 158 | 1 | 158 | 8.23e-109 | 306 |
| MS.gene059039.t1 | MTR_2g035150 | 86.076 | 158 | 22 | 0 | 1 | 158 | 1 | 158 | 1.10e-96 | 276 |
| MS.gene059039.t1 | MTR_2g035100 | 82.803 | 157 | 27 | 0 | 1 | 157 | 1 | 157 | 2.80e-93 | 267 |
| MS.gene059039.t1 | MTR_2g035130 | 84.076 | 157 | 25 | 0 | 1 | 157 | 1 | 157 | 2.99e-93 | 267 |
| MS.gene059039.t1 | MTR_2g035105 | 81.529 | 157 | 29 | 0 | 1 | 157 | 1 | 157 | 6.39e-92 | 264 |
| MS.gene059039.t1 | MTR_6g033450 | 68.354 | 158 | 50 | 0 | 1 | 158 | 1 | 158 | 3.32e-76 | 224 |
| MS.gene059039.t1 | MTR_4g120970 | 68.354 | 158 | 50 | 0 | 1 | 158 | 1 | 158 | 3.32e-76 | 224 |
| MS.gene059039.t1 | MTR_2g035320 | 59.236 | 157 | 64 | 0 | 1 | 157 | 1 | 157 | 7.32e-64 | 193 |
| MS.gene059039.t1 | MTR_2g035190 | 58.599 | 157 | 65 | 0 | 1 | 157 | 1 | 157 | 1.27e-62 | 190 |
| MS.gene059039.t1 | MTR_2g035170 | 57.325 | 157 | 67 | 0 | 1 | 157 | 1 | 157 | 7.63e-59 | 180 |
| MS.gene059039.t1 | MTR_2g035210 | 55.414 | 157 | 70 | 0 | 1 | 157 | 1 | 157 | 2.54e-58 | 179 |
| MS.gene059039.t1 | MTR_2g035220 | 57.325 | 157 | 67 | 0 | 1 | 157 | 1 | 157 | 3.45e-58 | 178 |
| MS.gene059039.t1 | MTR_2g035320 | 58.586 | 99 | 41 | 0 | 59 | 157 | 24 | 122 | 1.46e-36 | 122 |
| MS.gene059039.t1 | MTR_4g120760 | 38.217 | 157 | 96 | 1 | 1 | 156 | 1 | 157 | 2.44e-35 | 120 |
| MS.gene059039.t1 | MTR_4g120760 | 38.217 | 157 | 96 | 1 | 1 | 156 | 1 | 157 | 2.69e-35 | 120 |
| MS.gene059039.t1 | MTR_2g435310 | 36.943 | 157 | 98 | 1 | 1 | 156 | 1 | 157 | 4.07e-35 | 120 |
| MS.gene059039.t1 | MTR_4g120950 | 45.062 | 162 | 82 | 5 | 1 | 158 | 1 | 159 | 1.43e-33 | 116 |
| MS.gene059039.t1 | MTR_4g120940 | 71.622 | 74 | 21 | 0 | 85 | 158 | 47 | 120 | 1.11e-30 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTACAACTACAGCATAAT+TGG | 0.309137 | 2.4:-56062882 | MS.gene059039:CDS |
| AACGAAAGTGAGTTTCTTGA+TGG | 0.319436 | 2.4:+56063286 | None:intergenic |
| TTGATGACATCAACAACCTT+TGG | 0.332899 | 2.4:+56063347 | None:intergenic |
| GGCCCTTGAGGGTTACTGTT+TGG | 0.340986 | 2.4:-56062685 | MS.gene059039:CDS |
| TATATGCTTTAGTTGTAATC+AGG | 0.341112 | 2.4:+56062657 | None:intergenic |
| TGATGACATCAACAACCTTT+GGG | 0.365094 | 2.4:+56063348 | None:intergenic |
| ACTTGGTAGATGATGCTAAC+TGG | 0.375253 | 2.4:-56062905 | MS.gene059039:CDS |
| AGTGAGTTTCTTGATGGTTC+CGG | 0.377477 | 2.4:+56063292 | None:intergenic |
| GAAATCTTCTCTACTGTGTC+TGG | 0.378194 | 2.4:+56062846 | None:intergenic |
| CATCAACAACCTTTGGGATA+AGG | 0.380175 | 2.4:+56063354 | None:intergenic |
| AAGAAACTCACTTTCGTTGA+AGG | 0.384811 | 2.4:-56063281 | MS.gene059039:intron |
| CACTTTGTGTAAATCAAACT+TGG | 0.391922 | 2.4:+56062929 | None:intergenic |
| AAAAGTATTGACATTGTTGA+AGG | 0.419014 | 2.4:-56063326 | MS.gene059039:CDS |
| ATTGACATTGTTGAAGGAAA+TGG | 0.422286 | 2.4:-56063320 | MS.gene059039:CDS |
| CAAGTTTGATTTACACAAAG+TGG | 0.442873 | 2.4:-56062928 | MS.gene059039:CDS |
| GCTTTAGTTGTAATCAGGAT+TGG | 0.467799 | 2.4:+56062662 | None:intergenic |
| AAAGCTTTGTGAAGTGTAGC+AGG | 0.484835 | 2.4:+56063395 | None:intergenic |
| TTTGAAGCTAAATTGTCTGC+AGG | 0.489005 | 2.4:-56062822 | MS.gene059039:CDS |
| TTTGCAATGGATCCTCCATT+CGG | 0.495837 | 2.4:+56062798 | None:intergenic |
| ACTAGGAGTAGCATCACCTT+TGG | 0.504856 | 2.4:+56062755 | None:intergenic |
| CTTGGTAGATGATGCTAACT+GGG | 0.513123 | 2.4:-56062904 | MS.gene059039:CDS |
| CTTGAAAAGACCATCACCCT+TGG | 0.519688 | 2.4:+56062707 | None:intergenic |
| TTTCACACTAAGCTTTGCAA+TGG | 0.532493 | 2.4:+56062785 | None:intergenic |
| CTCTCGAGGTCCTCTTCACT+AGG | 0.534215 | 2.4:+56062738 | None:intergenic |
| GTTGAAGGAAATGGTGGCGC+CGG | 0.546300 | 2.4:-56063311 | MS.gene059039:CDS |
| TGATTTACACAAAGTGGACT+TGG | 0.554787 | 2.4:-56062922 | MS.gene059039:CDS |
| AGTGGCAAAGCCAAGGGTGA+TGG | 0.558120 | 2.4:-56062717 | MS.gene059039:CDS |
| TGTAGCAGGAGCTACAATAG+AGG | 0.565152 | 2.4:+56063409 | None:intergenic |
| CCTCGAGAGTGGCAAAGCCA+AGG | 0.566158 | 2.4:-56062724 | MS.gene059039:CDS |
| CTCGAGAGTGGCAAAGCCAA+GGG | 0.586680 | 2.4:-56062723 | MS.gene059039:CDS |
| CCTTGGCTTTGCCACTCTCG+AGG | 0.588424 | 2.4:+56062724 | None:intergenic |
| AAATTGTCTGCAGGTCCGAA+TGG | 0.591580 | 2.4:-56062813 | MS.gene059039:CDS |
| TACAACTACAGCATAATTGG+AGG | 0.598501 | 2.4:-56062879 | MS.gene059039:CDS |
| AGTGAAGAGGACCTCGAGAG+TGG | 0.617170 | 2.4:-56062735 | MS.gene059039:CDS |
| TGGCCAAACAGTAACCCTCA+AGG | 0.618520 | 2.4:+56062682 | None:intergenic |
| TGCTGACAACCTTATCCCAA+AGG | 0.624297 | 2.4:-56063363 | MS.gene059039:CDS |
| GGCCAAACAGTAACCCTCAA+GGG | 0.627034 | 2.4:+56062683 | None:intergenic |
| TGATGCTACTCCTAGTGAAG+AGG | 0.634756 | 2.4:-56062748 | MS.gene059039:CDS |
| TTGTCTGCAGGTCCGAATGG+AGG | 0.646240 | 2.4:-56062810 | MS.gene059039:CDS |
| AGTGTGAAATACTTCACCAA+AGG | 0.651010 | 2.4:-56062771 | MS.gene059039:CDS |
| GACATTGTTGAAGGAAATGG+TGG | 0.661697 | 2.4:-56063317 | MS.gene059039:CDS |
| AGCAGGAGCTACAATAGAGG+TGG | 0.665165 | 2.4:+56063412 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTAAATATATTAAGTAA+TGG | - | chr2.4:56063103-56063122 | MS.gene059039:intron | 15.0% |
| !! | AATCTTACTTATTAAGATTT+AGG | - | chr2.4:56062883-56062902 | MS.gene059039:CDS | 15.0% |
| !! | ATCTTACTTATTAAGATTTA+GGG | - | chr2.4:56062884-56062903 | MS.gene059039:CDS | 15.0% |
| !!! | ATATTTAGGTTTTTAAAAGT+AGG | + | chr2.4:56063094-56063113 | None:intergenic | 15.0% |
| !!! | TCATTTTCTCAAAATTTTAT+CGG | - | chr2.4:56063007-56063026 | MS.gene059039:intron | 15.0% |
| !! | GACCATTACTTAATATATTT+AGG | + | chr2.4:56063108-56063127 | None:intergenic | 20.0% |
| !!! | TTATTAAGATTTAGGGTTTA+GGG | - | chr2.4:56062891-56062910 | MS.gene059039:CDS | 20.0% |
| !! | CTTATTAAGATTTAGGGTTT+AGG | - | chr2.4:56062890-56062909 | MS.gene059039:CDS | 25.0% |
| !!! | AAAAGTATTGACATTGTTGA+AGG | - | chr2.4:56062779-56062798 | MS.gene059039:CDS | 25.0% |
| ATTGACATTGTTGAAGGAAA+TGG | - | chr2.4:56062785-56062804 | MS.gene059039:CDS | 30.0% | |
| CACTTTGTGTAAATCAAACT+TGG | + | chr2.4:56063179-56063198 | None:intergenic | 30.0% | |
| ! | CAAGTTTGATTTACACAAAG+TGG | - | chr2.4:56063177-56063196 | MS.gene059039:intron | 30.0% |
| ! | TTGTCTAAAACTATGAAGCA+CGG | + | chr2.4:56063062-56063081 | None:intergenic | 30.0% |
| AACGAAAGTGAGTTTCTTGA+TGG | + | chr2.4:56062822-56062841 | None:intergenic | 35.0% | |
| AAGAAACTCACTTTCGTTGA+AGG | - | chr2.4:56062824-56062843 | MS.gene059039:CDS | 35.0% | |
| AGTGTGAAATACTTCACCAA+AGG | - | chr2.4:56063334-56063353 | MS.gene059039:CDS | 35.0% | |
| ATGAATCTATGACATGCAGA+TGG | - | chr2.4:56063148-56063167 | MS.gene059039:intron | 35.0% | |
| GCTTACAACTACAGCATAAT+TGG | - | chr2.4:56063223-56063242 | MS.gene059039:intron | 35.0% | |
| TACAACTACAGCATAATTGG+AGG | - | chr2.4:56063226-56063245 | MS.gene059039:intron | 35.0% | |
| TGATGACATCAACAACCTTT+GGG | + | chr2.4:56062760-56062779 | None:intergenic | 35.0% | |
| TTTCACACTAAGCTTTGCAA+TGG | + | chr2.4:56063323-56063342 | None:intergenic | 35.0% | |
| ! | TGATTTACACAAAGTGGACT+TGG | - | chr2.4:56063183-56063202 | MS.gene059039:intron | 35.0% |
| ! | TTGATGACATCAACAACCTT+TGG | + | chr2.4:56062761-56062780 | None:intergenic | 35.0% |
| ! | TTTGAAGCTAAATTGTCTGC+AGG | - | chr2.4:56063283-56063302 | MS.gene059039:CDS | 35.0% |
| ACTTGGTAGATGATGCTAAC+TGG | - | chr2.4:56063200-56063219 | MS.gene059039:intron | 40.0% | |
| CTTGGTAGATGATGCTAACT+GGG | - | chr2.4:56063201-56063220 | MS.gene059039:intron | 40.0% | |
| GAAATCTTCTCTACTGTGTC+TGG | + | chr2.4:56063262-56063281 | None:intergenic | 40.0% | |
| GACATTGTTGAAGGAAATGG+TGG | - | chr2.4:56062788-56062807 | MS.gene059039:CDS | 40.0% | |
| TTTGCAATGGATCCTCCATT+CGG | + | chr2.4:56063310-56063329 | None:intergenic | 40.0% | |
| ! | AAAGCTTTGTGAAGTGTAGC+AGG | + | chr2.4:56062713-56062732 | None:intergenic | 40.0% |
| ! | AGTGAGTTTCTTGATGGTTC+CGG | + | chr2.4:56062816-56062835 | None:intergenic | 40.0% |
| !! | CATCAACAACCTTTGGGATA+AGG | + | chr2.4:56062754-56062773 | None:intergenic | 40.0% |
| AAATTGTCTGCAGGTCCGAA+TGG | - | chr2.4:56063292-56063311 | MS.gene059039:CDS | 45.0% | |
| ACTAGGAGTAGCATCACCTT+TGG | + | chr2.4:56063353-56063372 | None:intergenic | 45.0% | |
| CACGGACACCAATCACAATA+CGG | + | chr2.4:56063044-56063063 | None:intergenic | 45.0% | |
| CTTGAAAAGACCATCACCCT+TGG | + | chr2.4:56063401-56063420 | None:intergenic | 45.0% | |
| TCGTGTCAATGTCGAATGTC+AGG | + | chr2.4:56062963-56062982 | None:intergenic | 45.0% | |
| TGATGCGTCCGTATTGTGAT+TGG | - | chr2.4:56063033-56063052 | MS.gene059039:intron | 45.0% | |
| TGATGCTACTCCTAGTGAAG+AGG | - | chr2.4:56063357-56063376 | MS.gene059039:CDS | 45.0% | |
| TGCTGACAACCTTATCCCAA+AGG | - | chr2.4:56062742-56062761 | MS.gene059039:CDS | 45.0% | |
| TGTAGCAGGAGCTACAATAG+AGG | + | chr2.4:56062699-56062718 | None:intergenic | 45.0% | |
| !! | CAAGGGTGATGGTCTTTTCA+AGG | - | chr2.4:56063399-56063418 | MS.gene059039:CDS | 45.0% |
| AGCAGGAGCTACAATAGAGG+TGG | + | chr2.4:56062696-56062715 | None:intergenic | 50.0% | |
| GGCCAAACAGTAACCCTCAA+GGG | + | chr2.4:56063425-56063444 | None:intergenic | 50.0% | |
| TGGCCAAACAGTAACCCTCA+AGG | + | chr2.4:56063426-56063445 | None:intergenic | 50.0% | |
| ! | GCACCGACACTTCTGATTGA+CGG | - | chr2.4:56062932-56062951 | MS.gene059039:CDS | 50.0% |
| ! | GCACCGTCAATCAGAAGTGT+CGG | + | chr2.4:56062938-56062957 | None:intergenic | 50.0% |
| !! | CAGAAGTGTCGGTGCAACTT+AGG | + | chr2.4:56062927-56062946 | None:intergenic | 50.0% |
| !! | GGTCTTTTCAAGGCCCTTGA+GGG | - | chr2.4:56063409-56063428 | MS.gene059039:CDS | 50.0% |
| !! | TGGTCTTTTCAAGGCCCTTG+AGG | - | chr2.4:56063408-56063427 | MS.gene059039:CDS | 50.0% |
| AGTGAAGAGGACCTCGAGAG+TGG | - | chr2.4:56063370-56063389 | MS.gene059039:CDS | 55.0% | |
| AGTGGCAAAGCCAAGGGTGA+TGG | - | chr2.4:56063388-56063407 | MS.gene059039:CDS | 55.0% | |
| CTCGAGAGTGGCAAAGCCAA+GGG | - | chr2.4:56063382-56063401 | MS.gene059039:CDS | 55.0% | |
| CTCTCGAGGTCCTCTTCACT+AGG | + | chr2.4:56063370-56063389 | None:intergenic | 55.0% | |
| GGCCCTTGAGGGTTACTGTT+TGG | - | chr2.4:56063420-56063439 | MS.gene059039:CDS | 55.0% | |
| TTGTCTGCAGGTCCGAATGG+AGG | - | chr2.4:56063295-56063314 | MS.gene059039:CDS | 55.0% | |
| ! | GTTGAAGGAAATGGTGGCGC+CGG | - | chr2.4:56062794-56062813 | MS.gene059039:CDS | 55.0% |
| CCTCGAGAGTGGCAAAGCCA+AGG | - | chr2.4:56063381-56063400 | MS.gene059039:CDS | 60.0% | |
| !! | CCTTGGCTTTGCCACTCTCG+AGG | + | chr2.4:56063384-56063403 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 56062665 | 56063462 | 56062665 | ID=MS.gene059039 |
| chr2.4 | mRNA | 56062665 | 56063462 | 56062665 | ID=MS.gene059039.t1;Parent=MS.gene059039 |
| chr2.4 | exon | 56063282 | 56063462 | 56063282 | ID=MS.gene059039.t1.exon1;Parent=MS.gene059039.t1 |
| chr2.4 | CDS | 56063282 | 56063462 | 56063282 | ID=cds.MS.gene059039.t1;Parent=MS.gene059039.t1 |
| chr2.4 | exon | 56062665 | 56062960 | 56062665 | ID=MS.gene059039.t1.exon2;Parent=MS.gene059039.t1 |
| chr2.4 | CDS | 56062665 | 56062960 | 56062665 | ID=cds.MS.gene059039.t1;Parent=MS.gene059039.t1 |
| Gene Sequence |
| Protein sequence |
