AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39713


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39713.t1 MTR_8g007670 87.607 234 18 1 1 234 40 262 7.26e-150 417
MS.gene39713.t1 MTR_8g007670 64.103 234 29 4 1 234 40 218 2.12e-94 276
MS.gene39713.t1 MTR_8g007675 55.983 234 91 2 1 234 65 286 3.71e-87 259
MS.gene39713.t1 MTR_8g008660 50.427 234 101 3 1 234 60 278 3.05e-74 226
MS.gene39713.t1 MTR_8g008505 50.000 234 104 3 1 234 61 281 9.32e-74 225
MS.gene39713.t1 MTR_3g464040 48.718 234 105 3 1 234 52 270 2.14e-73 224
MS.gene39713.t1 MTR_8g008500 49.367 237 105 4 1 234 60 284 2.56e-72 222
MS.gene39713.t1 MTR_5g018300 40.426 235 126 4 1 234 41 262 2.06e-51 168
MS.gene39713.t1 MTR_5g018320 39.574 235 128 4 1 234 38 259 5.24e-51 167
MS.gene39713.t1 MTR_5g018280 36.555 238 130 5 1 234 41 261 6.66e-43 146
MS.gene39713.t1 MTR_5g018365 33.613 238 133 8 1 234 45 261 2.90e-32 118
MS.gene39713.t1 MTR_5g018350 33.333 237 135 5 1 234 40 256 1.16e-31 117
MS.gene39713.t1 MTR_8g079120 32.627 236 138 6 1 234 44 260 2.05e-31 116
MS.gene39713.t1 MTR_3g005410 29.167 240 137 8 1 231 127 342 3.56e-22 93.6
MS.gene39713.t1 MTR_8g079120 36.154 130 77 3 1 128 86 211 7.96e-19 82.0
MS.gene39713.t1 MTR_7g015220 28.017 232 149 5 1 231 157 371 2.56e-18 83.2
MS.gene39713.t1 MTR_5g008130 43.038 79 45 0 156 234 2 80 1.83e-16 72.0
MS.gene39713.t1 MTR_5g068100 27.602 221 142 5 1 220 63 266 4.37e-16 75.9
MS.gene39713.t1 MTR_3g093730 25.339 221 146 5 1 220 69 271 1.84e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39713.t1 AT3G50440 33.051 236 141 5 1 234 54 274 1.53e-33 122
MS.gene39713.t1 AT2G23590 31.602 231 136 7 1 231 60 268 5.42e-32 118
MS.gene39713.t1 AT2G23600 32.645 242 134 7 1 234 42 262 2.63e-31 116
MS.gene39713.t1 AT2G23600 32.645 242 134 7 1 234 57 277 3.61e-31 116
MS.gene39713.t1 AT2G23610 33.333 237 139 6 1 234 42 262 5.07e-29 110
MS.gene39713.t1 AT2G23620 32.231 242 135 8 1 234 42 262 5.71e-28 107
MS.gene39713.t1 AT5G10300 30.638 235 146 4 1 234 39 257 2.29e-25 100
MS.gene39713.t1 AT4G37150 29.362 235 150 5 1 234 36 255 1.10e-24 98.6
MS.gene39713.t1 AT1G33990 30.380 237 138 7 1 231 130 345 1.49e-24 100
MS.gene39713.t1 AT2G23550 29.412 238 148 6 1 234 41 262 7.09e-24 96.7
MS.gene39713.t1 AT2G23550 29.412 238 148 6 1 234 48 269 7.11e-24 96.7
MS.gene39713.t1 AT2G23580 29.958 237 148 6 1 234 41 262 1.57e-23 95.5
MS.gene39713.t1 AT4G09900 30.932 236 138 8 1 231 131 346 3.71e-22 93.6
MS.gene39713.t1 AT3G29770 30.172 232 144 5 1 231 171 385 6.29e-21 90.5
MS.gene39713.t1 AT2G23560 27.660 235 153 6 1 234 41 259 1.50e-20 87.8
MS.gene39713.t1 AT2G23600 35.294 136 74 4 1 128 57 186 1.55e-17 77.8
MS.gene39713.t1 AT1G26360 27.155 232 151 4 1 231 222 436 9.40e-17 79.0
MS.gene39713.t1 AT2G23550 28.713 202 125 5 36 234 48 233 1.32e-15 73.9
MS.gene39713.t1 AT1G69240 25.214 234 155 6 1 231 220 436 1.47e-15 75.5
MS.gene39713.t1 AT2G23610 40.000 100 58 2 1 99 42 140 2.80e-15 72.4
MS.gene39713.t1 AT4G37140 44.118 68 38 0 1 68 36 103 2.04e-14 68.2
MS.gene39713.t1 AT5G58310 24.268 239 155 5 1 231 38 258 2.44e-14 70.9
MS.gene39713.t1 AT3G10870 24.434 221 152 4 1 220 53 259 5.50e-14 70.1
MS.gene39713.t1 AT5G10300 44.118 68 38 0 1 68 39 106 4.43e-13 66.2
MS.gene39713.t1 AT4G16690 26.638 229 148 5 1 228 45 254 1.52e-12 65.9
MS.gene39713.t1 AT2G23570 29.293 198 113 9 44 234 1 178 4.86e-12 62.8
MS.gene39713.t1 AT2G23580 31.538 130 86 3 1 127 41 170 6.72e-12 63.2

Find 35 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCTTTGATGACATTTA+TGG 0.116749 8.1:+85974777 MS.gene39713:CDS
TAAATCAGCATCGCTCAAAA+AGG 0.284700 8.1:-85976026 None:intergenic
TGAACTTCTTGCATCTGCTT+TGG 0.342274 8.1:-85974736 None:intergenic
CAAATTCGATTTCTATATGC+AGG 0.415716 8.1:+85975959 MS.gene39713:CDS
GAGAGGATTGGAGCTTTGTC+TGG 0.423859 8.1:-85975778 None:intergenic
GTTCTTGTTGGTCGTAGTCT+TGG 0.427378 8.1:+85974826 MS.gene39713:CDS
TTACCTGAGATGGTGATAAC+TGG 0.434164 8.1:-85975837 None:intergenic
TTTGACACTTGCTTTATCTC+TGG 0.437434 8.1:+85975983 MS.gene39713:CDS
CTTGTTGGTCGTAGTCTTGG+AGG 0.446712 8.1:+85974829 MS.gene39713:CDS
GAGGAGAAAGTAGTTCTTGT+TGG 0.455636 8.1:+85974814 MS.gene39713:CDS
TGGTGATAACTGGTACATTC+TGG 0.466524 8.1:-85975827 None:intergenic
GACAAAATTTCATCATTGAC+GGG 0.468240 8.1:+85975755 MS.gene39713:CDS
GAGAAGAATAGGCTCAGCTT+TGG 0.471989 8.1:+85975729 MS.gene39713:CDS
GATCGAGGGTTCAGATCATA+TGG 0.477216 8.1:+85976192 MS.gene39713:CDS
ATGTAGCGAAGGAGAAGAAT+AGG 0.490240 8.1:+85975718 MS.gene39713:intron
CACTTGCTTTATCTCTGGTA+CGG 0.492239 8.1:+85975988 MS.gene39713:CDS
GTTGTCTGTGTCTATTGCTA+TGG 0.493669 8.1:+85974852 MS.gene39713:CDS
AGACAAAATTTCATCATTGA+CGG 0.494233 8.1:+85975754 MS.gene39713:CDS
TAAATGTCATCAAAGGTTGA+TGG 0.511909 8.1:-85974774 None:intergenic
GAATGGATAATTGAGACAAC+CGG 0.517534 8.1:+85976148 MS.gene39713:CDS
GTACCAGTTATCACCATCTC+AGG 0.520634 8.1:+85975834 MS.gene39713:CDS
AGTCCGTCAGACGAGAGGAT+TGG 0.527944 8.1:-85975790 None:intergenic
TATGGAGTCACTTCCTTGTG+AGG 0.541105 8.1:+85974795 MS.gene39713:CDS
TTGTTGGTCGTAGTCTTGGA+GGG 0.546131 8.1:+85974830 MS.gene39713:CDS
TTTCACTTTAGCATATGGAC+CGG 0.551824 8.1:-85976167 None:intergenic
GACTCCATAAATGTCATCAA+AGG 0.577498 8.1:-85974781 None:intergenic
GCTCCAATCCTCTCGTCTGA+CGG 0.581690 8.1:+85975787 MS.gene39713:CDS
TGCTAAAGTGAAAATGATCG+AGG 0.599047 8.1:+85976177 MS.gene39713:CDS
ACTGTTACAAATGAAAACAA+TGG 0.604853 8.1:+85976064 MS.gene39713:CDS
AATCGAGTCCGTCAGACGAG+AGG 0.609179 8.1:-85975795 None:intergenic
GAAGAAAAGCACGATAAGTG+AGG 0.624099 8.1:-85974936 None:intergenic
GCTAAAGTGAAAATGATCGA+GGG 0.635853 8.1:+85976178 MS.gene39713:CDS
AGAACTACTTTCTCCTCACA+AGG 0.635958 8.1:-85974808 None:intergenic
TACAAATGAAAACAATGGAA+TGG 0.636369 8.1:+85976069 MS.gene39713:CDS
GCACTAGACATGGCTGCTTG+TGG 0.636797 8.1:+85974706 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATTTAAGATTTTAAAAT+GGG + chr8.1:85975314-85975333 MS.gene39713:intron 10.0%
!!! TGATATTTAAGATTTTAAAA+TGG + chr8.1:85975313-85975332 MS.gene39713:intron 10.0%
!! ATTTGTAAGGAAAAAAAATT+AGG + chr8.1:85975065-85975084 MS.gene39713:intron 15.0%
!! CAAAACAAAAAAAAAAATAG+TGG + chr8.1:85975433-85975452 MS.gene39713:intron 15.0%
!! GAGAAAATATTGAAATTATT+TGG + chr8.1:85975497-85975516 MS.gene39713:intron 15.0%
!! TCAAAATACATAATATGAAT+TGG - chr8.1:85975873-85975892 None:intergenic 15.0%
!!! TAATTTTTTTTCCTTACAAA+TGG - chr8.1:85975066-85975085 None:intergenic 15.0%
!!! TTAAATATCATAATTTTCGA+AGG - chr8.1:85975303-85975322 None:intergenic 15.0%
!! AAAAATATTCATCAAATCAC+GGG - chr8.1:85975686-85975705 None:intergenic 20.0%
!! AGATTAGACAATTTATATTC+TGG - chr8.1:85975212-85975231 None:intergenic 20.0%
!! CAAAAATATTCATCAAATCA+CGG - chr8.1:85975687-85975706 None:intergenic 20.0%
!! TTGAAAAATAAGAATGATTG+AGG + chr8.1:85975402-85975421 MS.gene39713:intron 20.0%
!!! AAACAAAAGATTTTCAAGAA+TGG + chr8.1:85976131-85976150 MS.gene39713:CDS 20.0%
!!! TATATTCTGGCATTATATTT+TGG - chr8.1:85975199-85975218 None:intergenic 20.0%
!!! TTCAATATTTTCTCAAACTA+TGG - chr8.1:85975491-85975510 None:intergenic 20.0%
! ACTGTTACAAATGAAAACAA+TGG + chr8.1:85976064-85976083 MS.gene39713:CDS 25.0%
! AGACAAAATTTCATCATTGA+CGG + chr8.1:85975754-85975773 MS.gene39713:CDS 25.0%
! TACAAAAGTTGACACATAAA+CGG - chr8.1:85975168-85975187 None:intergenic 25.0%
! TACAAATGAAAACAATGGAA+TGG + chr8.1:85976069-85976088 MS.gene39713:CDS 25.0%
!! ATCATTTTCACTTTAGCATA+TGG - chr8.1:85976175-85976194 None:intergenic 25.0%
!! ATTTTTCTATTTACCTGAGA+TGG - chr8.1:85975850-85975869 None:intergenic 25.0%
!! CGTGATTTGATGAATATTTT+TGG + chr8.1:85975685-85975704 MS.gene39713:intron 25.0%
!! TACACTTTTGTATCAAAACT+TGG + chr8.1:85975141-85975160 MS.gene39713:intron 25.0%
AATAGTGGTATGTATCTACT+AGG + chr8.1:85975448-85975467 MS.gene39713:intron 30.0%
ACAGTTGTATGTGTGAATTT+AGG + chr8.1:85975607-85975626 MS.gene39713:intron 30.0%
CAAATTCGATTTCTATATGC+AGG + chr8.1:85975959-85975978 MS.gene39713:CDS 30.0%
CAGTTGTATGTGTGAATTTA+GGG + chr8.1:85975608-85975627 MS.gene39713:intron 30.0%
CATAACTACGTAAATTCAGT+AGG - chr8.1:85975116-85975135 None:intergenic 30.0%
GACAAAATTTCATCATTGAC+GGG + chr8.1:85975755-85975774 MS.gene39713:CDS 30.0%
GCCAATCAAAATACTCATTA+GGG + chr8.1:85975010-85975029 MS.gene39713:intron 30.0%
TAAATGTCATCAAAGGTTGA+TGG - chr8.1:85974777-85974796 None:intergenic 30.0%
TGCCAATCAAAATACTCATT+AGG + chr8.1:85975009-85975028 MS.gene39713:intron 30.0%
! TAGACTAAACACTTCTAGTT+AGG + chr8.1:85975567-85975586 MS.gene39713:intron 30.0%
! TCAACCTTTGATGACATTTA+TGG + chr8.1:85974777-85974796 MS.gene39713:CDS 30.0%
! TCAGAGATGATGAAGATTTT+AGG - chr8.1:85976097-85976116 None:intergenic 30.0%
! TTTCTCAAACTATGGACTAA+TGG - chr8.1:85975483-85975502 None:intergenic 30.0%
!! TGTTTTTATCACTTCGTCAA+AGG + chr8.1:85975634-85975653 MS.gene39713:intron 30.0%
!!! TCCCTAATGAGTATTTTGAT+TGG - chr8.1:85975014-85975033 None:intergenic 30.0%
!!! TGATGAATATTTTTGGTGTG+CGG + chr8.1:85975692-85975711 MS.gene39713:intron 30.0%
AAATCAGCATCGCTCAAAAA+GGG - chr8.1:85976028-85976047 None:intergenic 35.0%
CCTTACAAATGGAAAGTCAA+TGG - chr8.1:85975055-85975074 None:intergenic 35.0%
GAATGGATAATTGAGACAAC+CGG + chr8.1:85976148-85976167 MS.gene39713:CDS 35.0%
GACTCCATAAATGTCATCAA+AGG - chr8.1:85974784-85974803 None:intergenic 35.0%
GCTAAAGTGAAAATGATCGA+GGG + chr8.1:85976178-85976197 MS.gene39713:CDS 35.0%
TAAATCAGCATCGCTCAAAA+AGG - chr8.1:85976029-85976048 None:intergenic 35.0%
TGCTAAAGTGAAAATGATCG+AGG + chr8.1:85976177-85976196 MS.gene39713:CDS 35.0%
TTGTATGTGTGAATTTAGGG+TGG + chr8.1:85975611-85975630 MS.gene39713:intron 35.0%
TTTCACTTTAGCATATGGAC+CGG - chr8.1:85976170-85976189 None:intergenic 35.0%
TTTGACACTTGCTTTATCTC+TGG + chr8.1:85975983-85976002 MS.gene39713:CDS 35.0%
! ACTGCAACAAAGATTTTGTG+AGG - chr8.1:85974886-85974905 None:intergenic 35.0%
! CTGCAACAAAGATTTTGTGA+GGG - chr8.1:85974885-85974904 None:intergenic 35.0%
! TTATCGTGCTTTTCTTCAAG+AGG + chr8.1:85974942-85974961 MS.gene39713:CDS 35.0%
!! AGAGCTAAGTTTTGTAGGTT+TGG - chr8.1:85976227-85976246 None:intergenic 35.0%
!! AGTTCAGAGCTAAGTTTTGT+AGG - chr8.1:85976232-85976251 None:intergenic 35.0%
!! CCATTGACTTTCCATTTGTA+AGG + chr8.1:85975052-85975071 MS.gene39713:intron 35.0%
AGAACTACTTTCTCCTCACA+AGG - chr8.1:85974811-85974830 None:intergenic 40.0%
ATGTAGCGAAGGAGAAGAAT+AGG + chr8.1:85975718-85975737 MS.gene39713:intron 40.0%
CACTTGCTTTATCTCTGGTA+CGG + chr8.1:85975988-85976007 MS.gene39713:CDS 40.0%
GAGGAGAAAGTAGTTCTTGT+TGG + chr8.1:85974814-85974833 MS.gene39713:CDS 40.0%
GTTGTCTGTGTCTATTGCTA+TGG + chr8.1:85974852-85974871 MS.gene39713:CDS 40.0%
TGAACTTCTTGCATCTGCTT+TGG - chr8.1:85974739-85974758 None:intergenic 40.0%
TGGTGATAACTGGTACATTC+TGG - chr8.1:85975830-85975849 None:intergenic 40.0%
TTACCTGAGATGGTGATAAC+TGG - chr8.1:85975840-85975859 None:intergenic 40.0%
! GAAGAAAAGCACGATAAGTG+AGG - chr8.1:85974939-85974958 None:intergenic 40.0%
GAGAAGAATAGGCTCAGCTT+TGG + chr8.1:85975729-85975748 MS.gene39713:CDS 45.0%
GATCGAGGGTTCAGATCATA+TGG + chr8.1:85976192-85976211 MS.gene39713:CDS 45.0%
GTACCAGTTATCACCATCTC+AGG + chr8.1:85975834-85975853 MS.gene39713:CDS 45.0%
GTTCTTGTTGGTCGTAGTCT+TGG + chr8.1:85974826-85974845 MS.gene39713:CDS 45.0%
TATGGAGTCACTTCCTTGTG+AGG + chr8.1:85974795-85974814 MS.gene39713:CDS 45.0%
TCGCTCAAAAAGGGAGGTAA+TGG - chr8.1:85976019-85976038 None:intergenic 45.0%
! TTGTTGGTCGTAGTCTTGGA+GGG + chr8.1:85974830-85974849 MS.gene39713:CDS 45.0%
TCAGCATCGCTCAAAAAGGG+AGG - chr8.1:85976025-85976044 None:intergenic 50.0%
! CTTGTTGGTCGTAGTCTTGG+AGG + chr8.1:85974829-85974848 MS.gene39713:CDS 50.0%
! GAGAGGATTGGAGCTTTGTC+TGG - chr8.1:85975781-85975800 None:intergenic 50.0%
AATCGAGTCCGTCAGACGAG+AGG - chr8.1:85975798-85975817 None:intergenic 55.0%
AGTCCGTCAGACGAGAGGAT+TGG - chr8.1:85975793-85975812 None:intergenic 55.0%
GCTCCAATCCTCTCGTCTGA+CGG + chr8.1:85975787-85975806 MS.gene39713:CDS 55.0%
GTGTGCGGAACATGTAGCGA+AGG + chr8.1:85975707-85975726 MS.gene39713:intron 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 85974715 85976276 85974715 ID=MS.gene39713
chr8.1 mRNA 85974715 85976276 85974715 ID=MS.gene39713.t1;Parent=MS.gene39713
chr8.1 exon 85974715 85974963 85974715 ID=MS.gene39713.t1.exon1;Parent=MS.gene39713.t1
chr8.1 CDS 85974715 85974963 85974715 ID=cds.MS.gene39713.t1;Parent=MS.gene39713.t1
chr8.1 exon 85975724 85975855 85975724 ID=MS.gene39713.t1.exon2;Parent=MS.gene39713.t1
chr8.1 CDS 85975724 85975855 85975724 ID=cds.MS.gene39713.t1;Parent=MS.gene39713.t1
chr8.1 exon 85975953 85976276 85975953 ID=MS.gene39713.t1.exon3;Parent=MS.gene39713.t1
chr8.1 CDS 85975953 85976276 85975953 ID=cds.MS.gene39713.t1;Parent=MS.gene39713.t1
Gene Sequence

>MS.gene39713

ATGGCTGCTTGTGGTATCAATCCAAAGCAGATGCAAGAAGTTCATTCAATTTCTGAATACCATCAACCTTTGATGACATTTATGGAGTCACTTCCTTGTGAGGAGAAAGTAGTTCTTGTTGGTCGTAGTCTTGGAGGGTTGTCTGTGTCTATTGCTATGGAAAACTACCCTCACAAAATCTTTGTTGCAGTTTTCATCACAGCAACTGTTGTCACTCAAAACCTCACTTATCGTGCTTTTCTTCAAGAGGTACTTTGCATACAACGATATACTATTTGCATATTTACACTTTATTGCCAATCAAAATACTCATTAGGGACTTTGATCACAAGACATACCATTGACTTTCCATTTGTAAGGAAAAAAAATTAGGATTTGAATTCTATCAATAATAAAATCCTACTGAATTTACGTAGTTATGCGATCTACACTTTTGTATCAAAACTTGGACCGTTTATGTGTCAACTTTTGTAAATTGAGTCCAAAATATAATGCCAGAATATAAATTGTCTAATCTTAATATGACAGTTCAAATCATCGATAGTGTGATTGTATGAATGTCGAAAATCTAATCTAAAAGTTTTGCCTTCGAAAATTATGATATTTAAGATTTTAAAATGGGAAGTCAAACATACTAGAAAACAGTGTTAAATTCATATTGTAAAATAAGAATATGTTATCATAAAATTGAAAAATAAGAATGATTGAGGTGTATTAACAAAACAAAAAAAAAAATAGTGGTATGTATCTACTAGGTAGTAAGCACCATTAGTCCATAGTTTGAGAAAATATTGAAATTATTTGGAGTGTTATATCTTTATTGTATTATTCAATGACTAATGTCTATATTGTTAGACTAAACACTTCTAGTTAGGTTTGAATCAACGATCTCACAGTTGTATGTGTGAATTTAGGGTGGTGTTTTTATCACTTCGTCAAAGGAAAAACAATTACTCTTGTGAATTTGACCCGTGATTTGATGAATATTTTTGGTGTGCGGAACATGTAGCGAAGGAGAAGAATAGGCTCAGCTTTGGACAGACAAAATTTCATCATTGACGGGCCAGACAAAGCTCCAATCCTCTCGTCTGACGGACTCGATTTGTTAGCATCCAGAATGTACCAGTTATCACCATCTCAGGTAAATAGAAAAATCCAATTCATATTATGTATTTTGATTTGATTTTTAGCTGAAGTTTGTGTAAATATGATTTATATTGATTATTATTAATACTTAGTTAAAGCAAATTCGATTTCTATATGCAGGATTTGACACTTGCTTTATCTCTGGTACGGTGAGACCATTACCTCCCTTTTTGAGCGATGCTGATTTATTGATGAAACAAACAACTGTTACAAATGAAAACAATGGAATGGTGCCTAAAATCTTCATCATCTCTGAAAATGACAATTTGCAAACAAAAGATTTTCAAGAATGGATAATTGAGACAACCGGTCCATATGCTAAAGTGAAAATGATCGAGGGTTCAGATCATATGGTCATGCTCTCCAAACCTACAAAACTTAGCTCTGAACTTCTAAACATTTCTTACAATTATTAA

Protein sequence

>MS.gene39713.t1

MAACGINPKQMQEVHSISEYHQPLMTFMESLPCEEKVVLVGRSLGGLSVSIAMENYPHKIFVAVFITATVVTQNLTYRAFLQERRRRIGSALDRQNFIIDGPDKAPILSSDGLDLLASRMYQLSPSQLKQIRFLYAGFDTCFISGTVRPLPPFLSDADLLMKQTTVTNENNGMVPKIFIISENDNLQTKDFQEWIIETTGPYAKVKMIEGSDHMVMLSKPTKLSSELLNISYNY