Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40179.t1 | XP_003610640.1 | 94.5 | 73 | 2 | 1 | 1 | 73 | 1 | 71 | 2.80E-25 | 124 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40179.t1 | G7K0Z0 | 94.5 | 73 | 2 | 1 | 1 | 73 | 1 | 71 | 2.0e-25 | 124.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052533 | MS.gene40179 | 0.807461 | 5.00E-50 | -1.69E-46 |
| MS.gene053094 | MS.gene40179 | 0.808611 | 2.84E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene40179 | 0.801469 | 8.87E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene40179 | 0.810056 | 1.39E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene40179 | -0.830711 | 2.52E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40179.t1 | MTR_5g005330 | 94.521 | 73 | 2 | 1 | 1 | 73 | 1 | 71 | 2.13e-42 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40179.t1 | AT3G19660 | 56.667 | 60 | 23 | 1 | 1 | 60 | 1 | 57 | 7.68e-14 | 60.1 |
Find 15 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAAGAATAAGAAATTATA+TGG | 0.312903 | 5.4:+599773 | None:intergenic |
| ATTATTATCAGCAGCTTTCA+AGG | 0.340671 | 5.4:+599827 | None:intergenic |
| GGCAAATCAGCTGGGAAATC+TGG | 0.373442 | 5.4:-599985 | MS.gene40179:CDS |
| GACGTTGTGCTTGGTCTTAG+TGG | 0.439128 | 5.4:+599797 | None:intergenic |
| CTCTTGTCCATCTTTACGTA+TGG | 0.452531 | 5.4:+599906 | None:intergenic |
| TAGAAGAATGGCAAATCAGC+TGG | 0.458624 | 5.4:-599994 | None:intergenic |
| AAAGAAGCCATACGTAAAGA+TGG | 0.519030 | 5.4:-599913 | MS.gene40179:CDS |
| ACGTTGTGCTTGGTCTTAGT+GGG | 0.536017 | 5.4:+599798 | None:intergenic |
| TCAACGTTGAAGATGAAGAA+AGG | 0.541828 | 5.4:-599936 | MS.gene40179:CDS |
| TTATATGGTGACGTTGTGCT+TGG | 0.549241 | 5.4:+599788 | None:intergenic |
| GGTGGAGTCAATAAAGTCAA+AGG | 0.580028 | 5.4:-599964 | MS.gene40179:CDS |
| AGAAGAATGGCAAATCAGCT+GGG | 0.603317 | 5.4:-599993 | None:intergenic |
| TTGTGCTTGGTCTTAGTGGG+TGG | 0.613312 | 5.4:+599801 | None:intergenic |
| AAATCAGCTGGGAAATCTGG+TGG | 0.634337 | 5.4:-599982 | MS.gene40179:CDS |
| GGACAAGAGTGCAAGTGTGA+AGG | 0.667874 | 5.4:-599892 | MS.gene40179:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTATTATCAGCAGCTTTCA+AGG | + | chr5.4:599951-599970 | None:intergenic | 30.0% | |
| AAAGAAGCCATACGTAAAGA+TGG | - | chr5.4:599862-599881 | MS.gene40179:CDS | 35.0% | |
| TCAACGTTGAAGATGAAGAA+AGG | - | chr5.4:599839-599858 | MS.gene40179:CDS | 35.0% | |
| CTCTTGTCCATCTTTACGTA+TGG | + | chr5.4:599872-599891 | None:intergenic | 40.0% | |
| GGTGGAGTCAATAAAGTCAA+AGG | - | chr5.4:599811-599830 | MS.gene40179:CDS | 40.0% | |
| ! | TTATATGGTGACGTTGTGCT+TGG | + | chr5.4:599990-600009 | None:intergenic | 40.0% |
| AAATCAGCTGGGAAATCTGG+TGG | - | chr5.4:599793-599812 | MS.gene40179:CDS | 45.0% | |
| !! | ACGTTGTGCTTGGTCTTAGT+GGG | + | chr5.4:599980-599999 | None:intergenic | 45.0% |
| GGCAAATCAGCTGGGAAATC+TGG | - | chr5.4:599790-599809 | MS.gene40179:CDS | 50.0% | |
| ! | GGACAAGAGTGCAAGTGTGA+AGG | - | chr5.4:599883-599902 | MS.gene40179:CDS | 50.0% |
| !! | GACGTTGTGCTTGGTCTTAG+TGG | + | chr5.4:599981-600000 | None:intergenic | 50.0% |
| !! | TTGTGCTTGGTCTTAGTGGG+TGG | + | chr5.4:599977-599996 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 599788 | 600009 | 599788 | ID=MS.gene40179 |
| chr5.4 | mRNA | 599788 | 600009 | 599788 | ID=MS.gene40179.t1;Parent=MS.gene40179 |
| chr5.4 | exon | 599788 | 600009 | 599788 | ID=MS.gene40179.t1.exon1;Parent=MS.gene40179.t1 |
| chr5.4 | CDS | 599788 | 600009 | 599788 | ID=cds.MS.gene40179.t1;Parent=MS.gene40179.t1 |
| Gene Sequence |
| Protein sequence |
