Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40267.t1 | GAU14248.1 | 75.4 | 167 | 37 | 3 | 1 | 164 | 1 | 166 | 4.30E-69 | 270.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40267.t1 | A0A2K3NXU4 | 73.7 | 167 | 41 | 2 | 1 | 164 | 1 | 167 | 2.0e-68 | 268.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055124 | MS.gene40267 | 0.800633 | 1.32E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53620 | MS.gene40267 | PPI |
| MS.gene64289 | MS.gene40267 | PPI |
| MS.gene40267 | MS.gene064976 | PPI |
| MS.gene94983 | MS.gene40267 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40267.t1 | MTR_3g109880 | 60.335 | 179 | 55 | 5 | 1 | 169 | 1 | 173 | 3.85e-69 | 207 |
| MS.gene40267.t1 | MTR_6g088445 | 43.478 | 161 | 75 | 3 | 9 | 163 | 10 | 160 | 7.63e-38 | 127 |
| MS.gene40267.t1 | MTR_6g088475 | 43.038 | 158 | 74 | 3 | 9 | 160 | 10 | 157 | 3.41e-36 | 123 |
| MS.gene40267.t1 | MTR_1g046660 | 34.568 | 162 | 98 | 3 | 3 | 160 | 2 | 159 | 1.74e-24 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40267.t1 | AT4G32870 | 47.239 | 163 | 76 | 4 | 1 | 161 | 1 | 155 | 8.18e-49 | 155 |
| MS.gene40267.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 5.00e-31 | 110 |
| MS.gene40267.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 5.00e-31 | 110 |
Find 52 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATTGGAAACGTTATTA+AGG | 0.203448 | 3.3:-91074237 | MS.gene40267:CDS |
| TGAAATTGTTGATAACAGTA+TGG | 0.310256 | 3.3:-91074405 | MS.gene40267:CDS |
| AGGAAAATAAGCCAACAAAA+TGG | 0.322964 | 3.3:-91074667 | MS.gene40267:CDS |
| TTCATTGGAAACGTTATTAA+GGG | 0.346776 | 3.3:-91074236 | MS.gene40267:CDS |
| CAAGATTTGAATTCTTTCAT+TGG | 0.349097 | 3.3:-91074251 | MS.gene40267:CDS |
| TATAGTATGGCAAGTGTTAG+AGG | 0.403359 | 3.3:-91074603 | MS.gene40267:CDS |
| GGAAAATAAGCCAACAAAAT+GGG | 0.403599 | 3.3:-91074666 | MS.gene40267:CDS |
| TCTTTAACCCACTCAGAAGT+TGG | 0.415580 | 3.3:+91074470 | None:intergenic |
| ATGGATTGTTATAAGCTTGA+AGG | 0.449145 | 3.3:-91074551 | MS.gene40267:CDS |
| TCATCATCAACGCCGCTGAT+TGG | 0.462447 | 3.3:+91074341 | None:intergenic |
| TCAAGCTTATAACAATCCAT+TGG | 0.469517 | 3.3:+91074554 | None:intergenic |
| GGTGTTTGTGATCCGGTGGA+AGG | 0.474182 | 3.3:-91074284 | MS.gene40267:CDS |
| ACTGTTATCAACAATTTCAT+AGG | 0.476144 | 3.3:+91074409 | None:intergenic |
| TATAAGCTTGAAGGGATTGA+AGG | 0.477132 | 3.3:-91074542 | MS.gene40267:CDS |
| CAAGCTTATAACAATCCATT+GGG | 0.479296 | 3.3:+91074555 | None:intergenic |
| GAAATTGTTGATAACAGTAT+GGG | 0.485941 | 3.3:-91074404 | MS.gene40267:CDS |
| ATGGGAGGGTGTTTGTGATC+CGG | 0.489610 | 3.3:-91074291 | MS.gene40267:CDS |
| GAACTGAGGAGGATATAGTA+TGG | 0.492546 | 3.3:-91074616 | MS.gene40267:CDS |
| AACCCACTCAGAAGTTGGAT+TGG | 0.500294 | 3.3:+91074475 | None:intergenic |
| AAAGCCACCGCTGAGGTAAG+AGG | 0.507470 | 3.3:-91074638 | MS.gene40267:CDS |
| TGGATTGTTATAAGCTTGAA+GGG | 0.510009 | 3.3:-91074550 | MS.gene40267:CDS |
| ATAGGTCAAACAACGTTGGA+TGG | 0.513283 | 3.3:+91074427 | None:intergenic |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.3:-91074310 | MS.gene40267:CDS |
| GTGTTTGTGATCCGGTGGAA+GGG | 0.541416 | 3.3:-91074283 | MS.gene40267:CDS |
| TGGAAACGTTATTAAGGGCA+TGG | 0.549534 | 3.3:-91074231 | MS.gene40267:CDS |
| AGCGAGATCAATAATGGGAG+AGG | 0.553005 | 3.3:-91074687 | None:intergenic |
| TTTGTGATCCGGTGGAAGGG+TGG | 0.560676 | 3.3:-91074280 | MS.gene40267:CDS |
| TGAACTGTCCACCCTTCCAC+CGG | 0.560925 | 3.3:+91074272 | None:intergenic |
| GAAGGGATTGAAGGTCAACC+TGG | 0.564797 | 3.3:-91074533 | MS.gene40267:CDS |
| TCAACGCCGCTGATTGGCAA+TGG | 0.566877 | 3.3:+91074347 | None:intergenic |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.3:-91074309 | MS.gene40267:CDS |
| CCATTGGGAGCCACTTGTGT+AGG | 0.567437 | 3.3:+91074570 | None:intergenic |
| TAGGTCAAACAACGTTGGAT+GGG | 0.573010 | 3.3:+91074428 | None:intergenic |
| GCACAATAACGAACGAGACC+AGG | 0.573329 | 3.3:+91074515 | None:intergenic |
| AAATTGTTGATAACAGTATG+GGG | 0.598578 | 3.3:-91074403 | MS.gene40267:CDS |
| TTTCATAGGTCAAACAACGT+TGG | 0.600620 | 3.3:+91074423 | None:intergenic |
| TGCCAATCCAACTTCTGAGT+GGG | 0.606140 | 3.3:-91074477 | MS.gene40267:CDS |
| AGTTCCTCTTACCTCAGCGG+TGG | 0.616369 | 3.3:+91074634 | None:intergenic |
| CCTACACAAGTGGCTCCCAA+TGG | 0.622824 | 3.3:-91074570 | MS.gene40267:CDS |
| AATAAGCCAACAAAATGGGA+AGG | 0.626979 | 3.3:-91074662 | MS.gene40267:CDS |
| GGAGGGTGTTTGTGATCCGG+TGG | 0.630744 | 3.3:-91074288 | MS.gene40267:CDS |
| CGCTGAGGTAAGAGGAACTG+AGG | 0.632981 | 3.3:-91074630 | MS.gene40267:CDS |
| CTCAGTTCCTCTTACCTCAG+CGG | 0.641075 | 3.3:+91074631 | None:intergenic |
| AGGTTGTATGATCGAATGGG+AGG | 0.648114 | 3.3:-91074306 | MS.gene40267:CDS |
| GATGATGATGCTAAACGTGT+AGG | 0.650720 | 3.3:-91074326 | MS.gene40267:CDS |
| ATTTCTGTAACCTACACAAG+TGG | 0.658716 | 3.3:-91074580 | MS.gene40267:CDS |
| TGAGGTAAGAGGAACTGAGG+AGG | 0.667990 | 3.3:-91074627 | MS.gene40267:CDS |
| GGTTGTATGATCGAATGGGA+GGG | 0.676731 | 3.3:-91074305 | MS.gene40267:CDS |
| GTGCCAATCCAACTTCTGAG+TGG | 0.684174 | 3.3:-91074478 | MS.gene40267:CDS |
| GTATTGCCATTGCCAATCAG+CGG | 0.692711 | 3.3:-91074353 | MS.gene40267:CDS |
| TATGGGGTTTAAGAATTACG+TGG | 0.696481 | 3.3:-91074387 | MS.gene40267:CDS |
| GGAAGGTAAAGCCACCGCTG+AGG | 0.701441 | 3.3:-91074645 | MS.gene40267:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTGTTATCAACAATTTCAT+AGG | + | chr3.3:91074455-91074474 | None:intergenic | 25.0% |
| ! | CAAGATTTGAATTCTTTCAT+TGG | - | chr3.3:91074610-91074629 | MS.gene40267:CDS | 25.0% |
| ! | TATTGTGCTTTCACAAAAAA+GGG | - | chr3.3:91074361-91074380 | MS.gene40267:CDS | 25.0% |
| ! | TTATTGTGCTTTCACAAAAA+AGG | - | chr3.3:91074360-91074379 | MS.gene40267:CDS | 25.0% |
| ! | TTCATTGGAAACGTTATTAA+GGG | - | chr3.3:91074625-91074644 | MS.gene40267:CDS | 25.0% |
| ! | TTTCATTGGAAACGTTATTA+AGG | - | chr3.3:91074624-91074643 | MS.gene40267:CDS | 25.0% |
| !! | ATATGTAAGCTCAATTTTGT+TGG | + | chr3.3:91074656-91074675 | None:intergenic | 25.0% |
| !!! | AAATTGTTGATAACAGTATG+GGG | - | chr3.3:91074458-91074477 | MS.gene40267:CDS | 25.0% |
| !!! | GAAATTGTTGATAACAGTAT+GGG | - | chr3.3:91074457-91074476 | MS.gene40267:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAGTA+TGG | - | chr3.3:91074456-91074475 | MS.gene40267:CDS | 25.0% |
| AGGAAAATAAGCCAACAAAA+TGG | - | chr3.3:91074194-91074213 | MS.gene40267:CDS | 30.0% | |
| CAAGCTTATAACAATCCATT+GGG | + | chr3.3:91074309-91074328 | None:intergenic | 30.0% | |
| GGAAAATAAGCCAACAAAAT+GGG | - | chr3.3:91074195-91074214 | MS.gene40267:CDS | 30.0% | |
| TCAAGCTTATAACAATCCAT+TGG | + | chr3.3:91074310-91074329 | None:intergenic | 30.0% | |
| TGGATTGTTATAAGCTTGAA+GGG | - | chr3.3:91074311-91074330 | MS.gene40267:CDS | 30.0% | |
| ! | ATGGATTGTTATAAGCTTGA+AGG | - | chr3.3:91074310-91074329 | MS.gene40267:CDS | 30.0% |
| AATAAGCCAACAAAATGGGA+AGG | - | chr3.3:91074199-91074218 | MS.gene40267:CDS | 35.0% | |
| ATTTCTGTAACCTACACAAG+TGG | - | chr3.3:91074281-91074300 | MS.gene40267:CDS | 35.0% | |
| TATAAGCTTGAAGGGATTGA+AGG | - | chr3.3:91074319-91074338 | MS.gene40267:CDS | 35.0% | |
| TATAGTATGGCAAGTGTTAG+AGG | - | chr3.3:91074258-91074277 | MS.gene40267:CDS | 35.0% | |
| TATGGGGTTTAAGAATTACG+TGG | - | chr3.3:91074474-91074493 | MS.gene40267:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.3:91074552-91074571 | MS.gene40267:CDS | 35.0% | |
| TTTCATAGGTCAAACAACGT+TGG | + | chr3.3:91074441-91074460 | None:intergenic | 35.0% | |
| ATAGGTCAAACAACGTTGGA+TGG | + | chr3.3:91074437-91074456 | None:intergenic | 40.0% | |
| GAACTGAGGAGGATATAGTA+TGG | - | chr3.3:91074245-91074264 | MS.gene40267:CDS | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.3:91074551-91074570 | MS.gene40267:CDS | 40.0% | |
| TAGGTCAAACAACGTTGGAT+GGG | + | chr3.3:91074436-91074455 | None:intergenic | 40.0% | |
| TCTTTAACCCACTCAGAAGT+TGG | + | chr3.3:91074394-91074413 | None:intergenic | 40.0% | |
| TGGAAACGTTATTAAGGGCA+TGG | - | chr3.3:91074630-91074649 | MS.gene40267:CDS | 40.0% | |
| ! | GATGATGATGCTAAACGTGT+AGG | - | chr3.3:91074535-91074554 | MS.gene40267:CDS | 40.0% |
| ! | GCTTTACCTTCCCATTTTGT+TGG | + | chr3.3:91074208-91074227 | None:intergenic | 40.0% |
| AACCCACTCAGAAGTTGGAT+TGG | + | chr3.3:91074389-91074408 | None:intergenic | 45.0% | |
| AGGTTGTATGATCGAATGGG+AGG | - | chr3.3:91074555-91074574 | MS.gene40267:CDS | 45.0% | |
| GGTTGTATGATCGAATGGGA+GGG | - | chr3.3:91074556-91074575 | MS.gene40267:CDS | 45.0% | |
| GTATTGCCATTGCCAATCAG+CGG | - | chr3.3:91074508-91074527 | MS.gene40267:CDS | 45.0% | |
| TGCCAATCCAACTTCTGAGT+GGG | - | chr3.3:91074384-91074403 | MS.gene40267:CDS | 45.0% | |
| CTCAGTTCCTCTTACCTCAG+CGG | + | chr3.3:91074233-91074252 | None:intergenic | 50.0% | |
| GAAGGGATTGAAGGTCAACC+TGG | - | chr3.3:91074328-91074347 | MS.gene40267:CDS | 50.0% | |
| GCACAATAACGAACGAGACC+AGG | + | chr3.3:91074349-91074368 | None:intergenic | 50.0% | |
| GTGCCAATCCAACTTCTGAG+TGG | - | chr3.3:91074383-91074402 | MS.gene40267:CDS | 50.0% | |
| GTGTTTGTGATCCGGTGGAA+GGG | - | chr3.3:91074578-91074597 | MS.gene40267:CDS | 50.0% | |
| TCATCATCAACGCCGCTGAT+TGG | + | chr3.3:91074523-91074542 | None:intergenic | 50.0% | |
| TGAGGTAAGAGGAACTGAGG+AGG | - | chr3.3:91074234-91074253 | MS.gene40267:CDS | 50.0% | |
| !! | ATGGGAGGGTGTTTGTGATC+CGG | - | chr3.3:91074570-91074589 | MS.gene40267:CDS | 50.0% |
| AAAGCCACCGCTGAGGTAAG+AGG | - | chr3.3:91074223-91074242 | MS.gene40267:CDS | 55.0% | |
| AGTTCCTCTTACCTCAGCGG+TGG | + | chr3.3:91074230-91074249 | None:intergenic | 55.0% | |
| CCATTGGGAGCCACTTGTGT+AGG | + | chr3.3:91074294-91074313 | None:intergenic | 55.0% | |
| CCTACACAAGTGGCTCCCAA+TGG | - | chr3.3:91074291-91074310 | MS.gene40267:CDS | 55.0% | |
| CGCTGAGGTAAGAGGAACTG+AGG | - | chr3.3:91074231-91074250 | MS.gene40267:CDS | 55.0% | |
| GGTGTTTGTGATCCGGTGGA+AGG | - | chr3.3:91074577-91074596 | MS.gene40267:CDS | 55.0% | |
| TGAACTGTCCACCCTTCCAC+CGG | + | chr3.3:91074592-91074611 | None:intergenic | 55.0% | |
| TTTGTGATCCGGTGGAAGGG+TGG | - | chr3.3:91074581-91074600 | MS.gene40267:CDS | 55.0% | |
| ! | TCAACGCCGCTGATTGGCAA+TGG | + | chr3.3:91074517-91074536 | None:intergenic | 55.0% |
| GGAAGGTAAAGCCACCGCTG+AGG | - | chr3.3:91074216-91074235 | MS.gene40267:CDS | 60.0% | |
| !! | GGAGGGTGTTTGTGATCCGG+TGG | - | chr3.3:91074573-91074592 | MS.gene40267:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 91074187 | 91074696 | 91074187 | ID=MS.gene40267 |
| chr3.3 | mRNA | 91074187 | 91074696 | 91074187 | ID=MS.gene40267.t1;Parent=MS.gene40267 |
| chr3.3 | exon | 91074187 | 91074696 | 91074187 | ID=MS.gene40267.t1.exon1;Parent=MS.gene40267.t1 |
| chr3.3 | CDS | 91074187 | 91074696 | 91074187 | ID=cds.MS.gene40267.t1;Parent=MS.gene40267.t1 |
| Gene Sequence |
| Protein sequence |
