Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40716.t1 | GAU14248.1 | 75.4 | 167 | 37 | 3 | 1 | 164 | 1 | 166 | 4.30E-69 | 270.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40716.t1 | A0A2K3NXU4 | 73.7 | 167 | 41 | 2 | 1 | 164 | 1 | 167 | 2.0e-68 | 268.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055124 | MS.gene40716 | 0.811471 | 6.89E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53620 | MS.gene40716 | PPI |
| MS.gene64289 | MS.gene40716 | PPI |
| MS.gene40716 | MS.gene064976 | PPI |
| MS.gene94983 | MS.gene40716 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40716.t1 | MTR_3g109880 | 60.335 | 179 | 55 | 5 | 1 | 169 | 1 | 173 | 3.85e-69 | 207 |
| MS.gene40716.t1 | MTR_6g088445 | 43.478 | 161 | 75 | 3 | 9 | 163 | 10 | 160 | 7.63e-38 | 127 |
| MS.gene40716.t1 | MTR_6g088475 | 43.038 | 158 | 74 | 3 | 9 | 160 | 10 | 157 | 3.41e-36 | 123 |
| MS.gene40716.t1 | MTR_1g046660 | 34.568 | 162 | 98 | 3 | 3 | 160 | 2 | 159 | 1.74e-24 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40716.t1 | AT4G32870 | 47.239 | 163 | 76 | 4 | 1 | 161 | 1 | 155 | 8.18e-49 | 155 |
| MS.gene40716.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 5.00e-31 | 110 |
| MS.gene40716.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 5.00e-31 | 110 |
Find 52 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATTGGAAACGTTATTA+AGG | 0.203448 | 3.2:-88343816 | MS.gene40716:CDS |
| TGAAATTGTTGATAACAGTA+TGG | 0.310256 | 3.2:-88343984 | MS.gene40716:CDS |
| AGGAAAATAAGCCAACAAAA+TGG | 0.322964 | 3.2:-88344246 | MS.gene40716:CDS |
| TTCATTGGAAACGTTATTAA+GGG | 0.346776 | 3.2:-88343815 | MS.gene40716:CDS |
| CAAGATTTGAATTCTTTCAT+TGG | 0.349097 | 3.2:-88343830 | MS.gene40716:CDS |
| TATAGTATGGCAAGTGTTAG+AGG | 0.403359 | 3.2:-88344182 | MS.gene40716:CDS |
| GGAAAATAAGCCAACAAAAT+GGG | 0.403599 | 3.2:-88344245 | MS.gene40716:CDS |
| TCTTTAACCCACTCAGAAGT+TGG | 0.415580 | 3.2:+88344049 | None:intergenic |
| ATGGATTGTTATAAGCTTGA+AGG | 0.449145 | 3.2:-88344130 | MS.gene40716:CDS |
| TCATCATCAACGCCGCTGAT+TGG | 0.462447 | 3.2:+88343920 | None:intergenic |
| TCAAGCTTATAACAATCCAT+TGG | 0.469517 | 3.2:+88344133 | None:intergenic |
| GGTGTTTGTGATCCGGTGGA+AGG | 0.474182 | 3.2:-88343863 | MS.gene40716:CDS |
| ACTGTTATCAACAATTTCAT+AGG | 0.476144 | 3.2:+88343988 | None:intergenic |
| TATAAGCTTGAAGGGATTGA+AGG | 0.477132 | 3.2:-88344121 | MS.gene40716:CDS |
| CAAGCTTATAACAATCCATT+GGG | 0.479296 | 3.2:+88344134 | None:intergenic |
| GAAATTGTTGATAACAGTAT+GGG | 0.485941 | 3.2:-88343983 | MS.gene40716:CDS |
| ATGGGAGGGTGTTTGTGATC+CGG | 0.489610 | 3.2:-88343870 | MS.gene40716:CDS |
| GAACTGAGGAGGATATAGTA+TGG | 0.492546 | 3.2:-88344195 | MS.gene40716:CDS |
| AACCCACTCAGAAGTTGGAT+TGG | 0.500294 | 3.2:+88344054 | None:intergenic |
| AAAGCCACCGCTGAGGTAAG+AGG | 0.507470 | 3.2:-88344217 | MS.gene40716:CDS |
| TGGATTGTTATAAGCTTGAA+GGG | 0.510009 | 3.2:-88344129 | MS.gene40716:CDS |
| ATAGGTCAAACAACGTTGGA+TGG | 0.513283 | 3.2:+88344006 | None:intergenic |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.2:-88343889 | MS.gene40716:CDS |
| GTGTTTGTGATCCGGTGGAA+GGG | 0.541416 | 3.2:-88343862 | MS.gene40716:CDS |
| TGGAAACGTTATTAAGGGCA+TGG | 0.549534 | 3.2:-88343810 | MS.gene40716:CDS |
| TTTGTGATCCGGTGGAAGGG+TGG | 0.560676 | 3.2:-88343859 | MS.gene40716:CDS |
| TGAACTGTCCACCCTTCCAC+CGG | 0.560925 | 3.2:+88343851 | None:intergenic |
| GAAGGGATTGAAGGTCAACC+TGG | 0.564797 | 3.2:-88344112 | MS.gene40716:CDS |
| TCAACGCCGCTGATTGGCAA+TGG | 0.566877 | 3.2:+88343926 | None:intergenic |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.2:-88343888 | MS.gene40716:CDS |
| CCATTGGGAGCCACTTGTGT+AGG | 0.567437 | 3.2:+88344149 | None:intergenic |
| AGTGAGATCAATAATGGGAG+AGG | 0.570998 | 3.2:-88344266 | None:intergenic |
| TAGGTCAAACAACGTTGGAT+GGG | 0.573010 | 3.2:+88344007 | None:intergenic |
| GCACAATAACGAACGAGACC+AGG | 0.573329 | 3.2:+88344094 | None:intergenic |
| AAATTGTTGATAACAGTATG+GGG | 0.598578 | 3.2:-88343982 | MS.gene40716:CDS |
| TTTCATAGGTCAAACAACGT+TGG | 0.600620 | 3.2:+88344002 | None:intergenic |
| TGCCAATCCAACTTCTGAGT+GGG | 0.606140 | 3.2:-88344056 | MS.gene40716:CDS |
| AGTTCCTCTTACCTCAGCGG+TGG | 0.616369 | 3.2:+88344213 | None:intergenic |
| CCTACACAAGTGGCTCCCAA+TGG | 0.622824 | 3.2:-88344149 | MS.gene40716:CDS |
| AATAAGCCAACAAAATGGGA+AGG | 0.626979 | 3.2:-88344241 | MS.gene40716:CDS |
| GGAGGGTGTTTGTGATCCGG+TGG | 0.630744 | 3.2:-88343867 | MS.gene40716:CDS |
| CGCTGAGGTAAGAGGAACTG+AGG | 0.632981 | 3.2:-88344209 | MS.gene40716:CDS |
| CTCAGTTCCTCTTACCTCAG+CGG | 0.641075 | 3.2:+88344210 | None:intergenic |
| AGGTTGTATGATCGAATGGG+AGG | 0.648114 | 3.2:-88343885 | MS.gene40716:CDS |
| GATGATGATGCTAAACGTGT+AGG | 0.650720 | 3.2:-88343905 | MS.gene40716:CDS |
| ATTTCTGTAACCTACACAAG+TGG | 0.658716 | 3.2:-88344159 | MS.gene40716:CDS |
| TGAGGTAAGAGGAACTGAGG+AGG | 0.667990 | 3.2:-88344206 | MS.gene40716:CDS |
| GGTTGTATGATCGAATGGGA+GGG | 0.676731 | 3.2:-88343884 | MS.gene40716:CDS |
| GTGCCAATCCAACTTCTGAG+TGG | 0.684174 | 3.2:-88344057 | MS.gene40716:CDS |
| GTATTGCCATTGCCAATCAG+CGG | 0.692711 | 3.2:-88343932 | MS.gene40716:CDS |
| TATGGGGTTTAAGAATTACG+TGG | 0.696481 | 3.2:-88343966 | MS.gene40716:CDS |
| GGAAGGTAAAGCCACCGCTG+AGG | 0.701441 | 3.2:-88344224 | MS.gene40716:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTGTTATCAACAATTTCAT+AGG | + | chr3.2:88344034-88344053 | None:intergenic | 25.0% |
| ! | CAAGATTTGAATTCTTTCAT+TGG | - | chr3.2:88344189-88344208 | MS.gene40716:CDS | 25.0% |
| ! | TATTGTGCTTTCACAAAAAA+GGG | - | chr3.2:88343940-88343959 | MS.gene40716:CDS | 25.0% |
| ! | TTATTGTGCTTTCACAAAAA+AGG | - | chr3.2:88343939-88343958 | MS.gene40716:CDS | 25.0% |
| ! | TTCATTGGAAACGTTATTAA+GGG | - | chr3.2:88344204-88344223 | MS.gene40716:CDS | 25.0% |
| ! | TTTCATTGGAAACGTTATTA+AGG | - | chr3.2:88344203-88344222 | MS.gene40716:CDS | 25.0% |
| !! | ATATGTAAGCTCAATTTTGT+TGG | + | chr3.2:88344235-88344254 | None:intergenic | 25.0% |
| !!! | AAATTGTTGATAACAGTATG+GGG | - | chr3.2:88344037-88344056 | MS.gene40716:CDS | 25.0% |
| !!! | GAAATTGTTGATAACAGTAT+GGG | - | chr3.2:88344036-88344055 | MS.gene40716:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAGTA+TGG | - | chr3.2:88344035-88344054 | MS.gene40716:CDS | 25.0% |
| AGGAAAATAAGCCAACAAAA+TGG | - | chr3.2:88343773-88343792 | MS.gene40716:CDS | 30.0% | |
| CAAGCTTATAACAATCCATT+GGG | + | chr3.2:88343888-88343907 | None:intergenic | 30.0% | |
| GGAAAATAAGCCAACAAAAT+GGG | - | chr3.2:88343774-88343793 | MS.gene40716:CDS | 30.0% | |
| TCAAGCTTATAACAATCCAT+TGG | + | chr3.2:88343889-88343908 | None:intergenic | 30.0% | |
| TGGATTGTTATAAGCTTGAA+GGG | - | chr3.2:88343890-88343909 | MS.gene40716:CDS | 30.0% | |
| ! | ATGGATTGTTATAAGCTTGA+AGG | - | chr3.2:88343889-88343908 | MS.gene40716:CDS | 30.0% |
| AATAAGCCAACAAAATGGGA+AGG | - | chr3.2:88343778-88343797 | MS.gene40716:CDS | 35.0% | |
| ATTTCTGTAACCTACACAAG+TGG | - | chr3.2:88343860-88343879 | MS.gene40716:CDS | 35.0% | |
| TATAAGCTTGAAGGGATTGA+AGG | - | chr3.2:88343898-88343917 | MS.gene40716:CDS | 35.0% | |
| TATAGTATGGCAAGTGTTAG+AGG | - | chr3.2:88343837-88343856 | MS.gene40716:CDS | 35.0% | |
| TATGGGGTTTAAGAATTACG+TGG | - | chr3.2:88344053-88344072 | MS.gene40716:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.2:88344131-88344150 | MS.gene40716:CDS | 35.0% | |
| TTTCATAGGTCAAACAACGT+TGG | + | chr3.2:88344020-88344039 | None:intergenic | 35.0% | |
| ATAGGTCAAACAACGTTGGA+TGG | + | chr3.2:88344016-88344035 | None:intergenic | 40.0% | |
| GAACTGAGGAGGATATAGTA+TGG | - | chr3.2:88343824-88343843 | MS.gene40716:CDS | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.2:88344130-88344149 | MS.gene40716:CDS | 40.0% | |
| TAGGTCAAACAACGTTGGAT+GGG | + | chr3.2:88344015-88344034 | None:intergenic | 40.0% | |
| TCTTTAACCCACTCAGAAGT+TGG | + | chr3.2:88343973-88343992 | None:intergenic | 40.0% | |
| TGGAAACGTTATTAAGGGCA+TGG | - | chr3.2:88344209-88344228 | MS.gene40716:CDS | 40.0% | |
| ! | GATGATGATGCTAAACGTGT+AGG | - | chr3.2:88344114-88344133 | MS.gene40716:CDS | 40.0% |
| ! | GCTTTACCTTCCCATTTTGT+TGG | + | chr3.2:88343787-88343806 | None:intergenic | 40.0% |
| AACCCACTCAGAAGTTGGAT+TGG | + | chr3.2:88343968-88343987 | None:intergenic | 45.0% | |
| AGGTTGTATGATCGAATGGG+AGG | - | chr3.2:88344134-88344153 | MS.gene40716:CDS | 45.0% | |
| GGTTGTATGATCGAATGGGA+GGG | - | chr3.2:88344135-88344154 | MS.gene40716:CDS | 45.0% | |
| GTATTGCCATTGCCAATCAG+CGG | - | chr3.2:88344087-88344106 | MS.gene40716:CDS | 45.0% | |
| TGCCAATCCAACTTCTGAGT+GGG | - | chr3.2:88343963-88343982 | MS.gene40716:CDS | 45.0% | |
| CTCAGTTCCTCTTACCTCAG+CGG | + | chr3.2:88343812-88343831 | None:intergenic | 50.0% | |
| GAAGGGATTGAAGGTCAACC+TGG | - | chr3.2:88343907-88343926 | MS.gene40716:CDS | 50.0% | |
| GCACAATAACGAACGAGACC+AGG | + | chr3.2:88343928-88343947 | None:intergenic | 50.0% | |
| GTGCCAATCCAACTTCTGAG+TGG | - | chr3.2:88343962-88343981 | MS.gene40716:CDS | 50.0% | |
| GTGTTTGTGATCCGGTGGAA+GGG | - | chr3.2:88344157-88344176 | MS.gene40716:CDS | 50.0% | |
| TCATCATCAACGCCGCTGAT+TGG | + | chr3.2:88344102-88344121 | None:intergenic | 50.0% | |
| TGAGGTAAGAGGAACTGAGG+AGG | - | chr3.2:88343813-88343832 | MS.gene40716:CDS | 50.0% | |
| !! | ATGGGAGGGTGTTTGTGATC+CGG | - | chr3.2:88344149-88344168 | MS.gene40716:CDS | 50.0% |
| AAAGCCACCGCTGAGGTAAG+AGG | - | chr3.2:88343802-88343821 | MS.gene40716:CDS | 55.0% | |
| AGTTCCTCTTACCTCAGCGG+TGG | + | chr3.2:88343809-88343828 | None:intergenic | 55.0% | |
| CCATTGGGAGCCACTTGTGT+AGG | + | chr3.2:88343873-88343892 | None:intergenic | 55.0% | |
| CCTACACAAGTGGCTCCCAA+TGG | - | chr3.2:88343870-88343889 | MS.gene40716:CDS | 55.0% | |
| CGCTGAGGTAAGAGGAACTG+AGG | - | chr3.2:88343810-88343829 | MS.gene40716:CDS | 55.0% | |
| GGTGTTTGTGATCCGGTGGA+AGG | - | chr3.2:88344156-88344175 | MS.gene40716:CDS | 55.0% | |
| TGAACTGTCCACCCTTCCAC+CGG | + | chr3.2:88344171-88344190 | None:intergenic | 55.0% | |
| TTTGTGATCCGGTGGAAGGG+TGG | - | chr3.2:88344160-88344179 | MS.gene40716:CDS | 55.0% | |
| ! | TCAACGCCGCTGATTGGCAA+TGG | + | chr3.2:88344096-88344115 | None:intergenic | 55.0% |
| GGAAGGTAAAGCCACCGCTG+AGG | - | chr3.2:88343795-88343814 | MS.gene40716:CDS | 60.0% | |
| !! | GGAGGGTGTTTGTGATCCGG+TGG | - | chr3.2:88344152-88344171 | MS.gene40716:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 88343766 | 88344275 | 88343766 | ID=MS.gene40716 |
| chr3.2 | mRNA | 88343766 | 88344275 | 88343766 | ID=MS.gene40716.t1;Parent=MS.gene40716 |
| chr3.2 | exon | 88343766 | 88344275 | 88343766 | ID=MS.gene40716.t1.exon1;Parent=MS.gene40716.t1 |
| chr3.2 | CDS | 88343766 | 88344275 | 88343766 | ID=cds.MS.gene40716.t1;Parent=MS.gene40716.t1 |
| Gene Sequence |
| Protein sequence |
