AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40749

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40749.t1 MTR_1g084820 94.945 633 32 0 1 633 1 633 0.0 1019
MS.gene40749.t1 MTR_1g084820 94.571 571 31 0 1 571 1 571 0.0 900
MS.gene40749.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 3.84e-56 193
MS.gene40749.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 6.11e-56 190
MS.gene40749.t1 MTR_7g111600 63.402 194 50 8 318 503 64 244 1.76e-55 191
MS.gene40749.t1 MTR_3g048280 47.468 158 60 4 346 502 60 195 7.36e-36 134
MS.gene40749.t1 MTR_5g091650 53.636 110 50 1 402 510 90 199 4.16e-31 122
MS.gene40749.t1 MTR_5g091650 56.383 94 40 1 413 505 101 194 1.11e-29 116
MS.gene40749.t1 MTR_2g049670 41.121 214 83 6 331 511 46 249 1.85e-29 119
MS.gene40749.t1 MTR_2g049670 48.031 127 49 2 420 546 158 267 3.13e-25 107
MS.gene40749.t1 MTR_8g089400 53.636 110 47 3 405 512 24 131 1.39e-28 112
MS.gene40749.t1 MTR_8g089400 53.636 110 47 3 405 512 44 151 1.95e-28 112
MS.gene40749.t1 MTR_1g056640 57.609 92 37 2 416 505 402 493 1.64e-27 117
MS.gene40749.t1 MTR_5g015720 25.617 527 254 18 1 502 1 414 3.00e-27 115
MS.gene40749.t1 MTR_8g085390 45.038 131 60 2 419 548 29 148 6.60e-27 108
MS.gene40749.t1 MTR_4g132280 50.485 103 49 2 412 512 374 476 4.34e-26 112
MS.gene40749.t1 MTR_8g085440 51.685 89 42 1 416 503 28 116 2.43e-25 101
MS.gene40749.t1 MTR_8g088120 62.821 78 28 1 429 505 57 134 2.62e-25 105
MS.gene40749.t1 MTR_8g085400 59.494 79 30 1 418 496 31 107 3.30e-24 98.2
MS.gene40749.t1 MTR_5g085580 52.128 94 43 2 414 505 376 469 3.09e-22 100
MS.gene40749.t1 MTR_8g085420 48.810 84 42 1 416 498 28 111 9.22e-22 92.0
MS.gene40749.t1 MTR_3g083580 46.000 100 52 2 418 515 360 459 9.90e-22 99.4
MS.gene40749.t1 MTR_5g097420 51.613 93 43 2 412 502 22 114 1.25e-21 90.9
MS.gene40749.t1 MTR_8g085720 47.368 95 48 2 420 512 364 458 1.10e-20 96.3
MS.gene40749.t1 MTR_5g078200 47.917 96 48 2 412 505 319 414 1.49e-20 96.3
MS.gene40749.t1 MTR_5g078200 47.917 96 48 2 412 505 352 447 1.52e-20 96.3
MS.gene40749.t1 MTR_6g088885 38.739 111 65 2 408 515 357 467 1.86e-20 95.5
MS.gene40749.t1 MTR_3g082080 53.846 78 35 1 420 496 157 234 2.23e-20 91.3
MS.gene40749.t1 MTR_4g083500 41.000 100 58 1 418 516 400 499 2.56e-20 95.1
MS.gene40749.t1 MTR_1g022160 47.778 90 46 1 419 507 390 479 3.25e-20 94.7
MS.gene40749.t1 MTR_4g079530 44.554 101 54 2 410 508 27 127 6.44e-20 86.3
MS.gene40749.t1 MTR_3g065460 40.984 122 70 2 397 516 338 459 1.15e-19 92.8
MS.gene40749.t1 MTR_4g109490 52.941 85 38 2 419 502 23 106 1.24e-19 88.6
MS.gene40749.t1 MTR_4g133735 48.352 91 45 2 414 502 22 112 1.34e-19 85.1
MS.gene40749.t1 MTR_1g116870 45.455 88 47 1 416 502 272 359 1.40e-19 91.3
MS.gene40749.t1 MTR_4g079530 49.474 95 46 2 420 512 37 131 1.65e-19 87.0
MS.gene40749.t1 MTR_5g044530 50.000 92 43 2 417 505 19 110 1.67e-19 89.0
MS.gene40749.t1 MTR_4g083070 50.000 88 42 2 420 505 21 108 5.72e-19 85.9
MS.gene40749.t1 MTR_1g022160 48.235 85 43 1 419 502 390 474 1.49e-18 89.4
MS.gene40749.t1 MTR_8g102340 38.182 110 66 2 419 526 333 442 1.62e-18 89.4
MS.gene40749.t1 MTR_4g133730 46.154 91 47 2 414 502 22 112 1.76e-18 82.0
MS.gene40749.t1 MTR_4g069940 45.133 113 53 4 403 507 353 464 1.77e-18 89.0
MS.gene40749.t1 MTR_2g072500 47.674 86 43 2 418 502 6 90 1.95e-18 80.9
MS.gene40749.t1 MTR_1g031900 40.517 116 67 2 418 531 359 474 4.11e-18 88.2
MS.gene40749.t1 MTR_1g031900 48.276 87 43 2 418 502 359 445 1.02e-17 87.0
MS.gene40749.t1 MTR_1g022160 47.500 80 41 1 419 497 390 469 3.42e-17 85.1
MS.gene40749.t1 MTR_8g012400 39.216 102 55 3 405 504 364 460 2.28e-16 82.4
MS.gene40749.t1 MTR_8g012400 39.216 102 55 3 405 504 284 380 2.29e-16 82.0
MS.gene40749.t1 MTR_7g053500 42.478 113 60 5 395 504 273 383 4.05e-16 81.3
MS.gene40749.t1 MTR_3g116510 42.202 109 58 3 403 507 218 325 6.72e-16 79.7
MS.gene40749.t1 MTR_7g053500 42.478 113 60 5 395 504 350 460 6.92e-16 80.9
MS.gene40749.t1 MTR_3g116510 42.202 109 58 3 403 507 352 459 8.79e-16 80.9
MS.gene40749.t1 MTR_7g026340 38.235 102 62 1 400 500 345 446 5.67e-14 75.1
MS.gene40749.t1 MTR_4g088155 39.623 106 57 4 402 504 358 459 1.64e-13 73.6
MS.gene40749.t1 MTR_3g031770 31.333 150 87 5 400 535 342 489 1.46e-12 70.9
MS.gene40749.t1 MTR_1g053320 35.849 106 58 5 418 518 372 472 7.79e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40749.t1 AT1G09460 69.048 126 36 2 386 509 101 225 9.31e-44 160
MS.gene40749.t1 AT2G30933 59.574 94 37 1 418 510 80 173 4.47e-33 127
MS.gene40749.t1 AT1G29380 64.444 90 31 1 420 508 148 237 1.60e-31 124
MS.gene40749.t1 AT1G29380 65.476 84 28 1 420 502 148 231 3.04e-30 121
MS.gene40749.t1 AT2G30933 43.846 130 41 2 368 496 61 159 8.72e-29 112
MS.gene40749.t1 AT2G30933 43.846 130 41 2 368 496 61 159 8.72e-29 112
MS.gene40749.t1 AT1G29380 66.667 78 25 1 420 496 148 225 2.08e-28 114
MS.gene40749.t1 AT1G29380 66.667 78 25 1 420 496 148 225 2.08e-28 114
MS.gene40749.t1 AT2G05790 53.448 116 49 3 395 505 356 471 2.96e-28 119
MS.gene40749.t1 AT1G11820 51.515 99 46 2 418 514 379 477 1.58e-25 111
MS.gene40749.t1 AT4G13600 44.094 127 66 3 420 542 61 186 2.13e-25 105
MS.gene40749.t1 AT4G05430 44.444 117 63 2 400 514 3 119 2.65e-25 102
MS.gene40749.t1 AT4G05430 44.167 120 65 2 397 514 25 144 7.05e-25 102
MS.gene40749.t1 AT5G55180 52.632 95 43 2 413 505 365 459 1.31e-24 107
MS.gene40749.t1 AT4G26830 35.751 193 110 6 322 505 267 454 1.06e-23 105
MS.gene40749.t1 AT3G55430 46.739 92 48 1 412 502 358 449 1.27e-23 104
MS.gene40749.t1 AT4G26830 35.751 193 110 6 322 505 271 458 1.55e-23 104
MS.gene40749.t1 AT5G55180 55.814 86 36 2 413 496 365 450 2.60e-22 100
MS.gene40749.t1 AT5G35740 52.747 91 40 3 415 502 25 115 2.88e-22 92.8
MS.gene40749.t1 AT2G01630 39.535 129 74 3 418 544 358 484 1.57e-21 99.0
MS.gene40749.t1 AT4G34480 47.674 86 44 1 419 503 252 337 1.57e-21 97.8
MS.gene40749.t1 AT1G18650 47.778 90 46 1 419 507 20 109 2.04e-21 92.8
MS.gene40749.t1 AT4G34480 47.674 86 44 1 419 503 363 448 2.56e-21 98.2
MS.gene40749.t1 AT3G58100 47.872 94 47 2 420 511 41 134 5.71e-21 91.3
MS.gene40749.t1 AT1G11820 54.321 81 35 2 418 496 379 459 6.86e-21 96.7
MS.gene40749.t1 AT3G58100 46.939 98 50 2 416 511 113 210 9.00e-21 92.8
MS.gene40749.t1 AT1G18650 47.191 89 46 1 419 506 20 108 1.35e-20 88.6
MS.gene40749.t1 AT4G05430 46.078 102 53 2 397 496 25 126 1.51e-20 88.2
MS.gene40749.t1 AT1G79480 50.000 84 41 1 420 502 310 393 3.66e-20 93.6
MS.gene40749.t1 AT1G79480 50.000 84 41 1 420 502 311 394 3.66e-20 93.6
MS.gene40749.t1 AT1G66250 39.474 114 66 3 418 529 366 478 5.58e-20 94.0
MS.gene40749.t1 AT2G16230 39.806 103 61 1 419 520 365 467 1.14e-19 93.2
MS.gene40749.t1 AT5G61130 47.674 86 44 1 419 503 20 105 1.34e-19 87.8
MS.gene40749.t1 AT1G69295 56.338 71 29 2 419 488 20 89 2.43e-19 87.8
MS.gene40749.t1 AT1G69295 56.338 71 29 2 419 488 16 85 4.23e-19 87.0
MS.gene40749.t1 AT5G67460 48.235 85 42 2 420 502 293 377 4.46e-19 90.1
MS.gene40749.t1 AT5G56590 40.777 103 59 2 420 520 369 471 9.86e-19 90.1
MS.gene40749.t1 AT1G26450 38.312 154 67 6 421 550 22 171 1.06e-18 85.1
MS.gene40749.t1 AT5G63225 46.512 86 45 1 418 502 23 108 1.43e-18 82.0
MS.gene40749.t1 AT1G66870 42.529 87 48 2 418 502 51 137 2.52e-18 82.4
MS.gene40749.t1 AT1G66870 42.529 87 48 2 418 502 24 110 2.80e-18 81.3
MS.gene40749.t1 AT5G67460 50.633 79 37 2 420 496 293 371 2.88e-18 87.8
MS.gene40749.t1 AT4G29360 45.977 87 45 2 418 502 399 485 3.00e-18 89.0
MS.gene40749.t1 AT4G29360 45.977 87 45 2 418 502 389 475 3.09e-18 89.0
MS.gene40749.t1 AT4G09462 41.758 91 52 1 415 504 24 114 4.44e-18 80.9
MS.gene40749.t1 AT5G24318 36.697 109 62 2 398 505 217 319 5.18e-18 85.9
MS.gene40749.t1 AT5G24318 36.697 109 62 2 398 505 356 458 5.83e-18 87.4
MS.gene40749.t1 AT4G09465 40.659 91 53 1 415 504 26 116 8.56e-18 80.1
MS.gene40749.t1 AT5G08000 44.262 122 66 2 417 536 37 158 9.33e-18 82.8
MS.gene40749.t1 AT4G09467 40.659 91 53 1 415 504 26 116 1.05e-17 79.7
MS.gene40749.t1 AT4G09464 40.659 91 53 1 415 504 26 116 1.05e-17 79.7
MS.gene40749.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene40749.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene40749.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene40749.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene40749.t1 AT3G13560 43.434 99 54 2 420 516 362 460 1.14e-17 87.0
MS.gene40749.t1 AT4G09466 40.659 91 53 1 415 504 26 116 2.22e-17 79.0
MS.gene40749.t1 AT2G42930 44.872 78 43 0 420 497 40 117 2.93e-17 79.0
MS.gene40749.t1 AT4G09090 43.023 86 48 1 420 504 31 116 3.21e-17 78.6
MS.gene40749.t1 AT2G01630 48.148 81 40 2 418 496 358 438 3.54e-17 85.1
MS.gene40749.t1 AT1G66852 39.535 86 51 1 418 502 24 109 5.48e-17 77.4
MS.gene40749.t1 AT3G23770 44.118 102 53 4 405 504 374 473 9.54e-17 84.0
MS.gene40749.t1 AT5G08000 47.674 86 44 1 419 503 20 105 9.65e-17 79.3
MS.gene40749.t1 AT1G78520 40.000 90 52 2 414 502 26 114 1.67e-16 76.3
MS.gene40749.t1 AT2G04910 43.182 88 48 2 415 500 10 97 1.87e-16 75.9
MS.gene40749.t1 AT5G63240 36.275 102 61 2 407 504 27 128 7.00e-16 75.1
MS.gene40749.t1 AT5G24318 37.000 100 56 2 398 496 356 449 1.32e-15 80.1
MS.gene40749.t1 AT5G63230 37.363 91 56 1 413 502 19 109 3.97e-15 72.4
MS.gene40749.t1 AT1G66855 37.931 87 52 2 418 502 24 110 6.15e-15 71.6
MS.gene40749.t1 AT5G63250 38.776 98 55 3 412 504 33 130 8.61e-15 72.0
MS.gene40749.t1 AT4G16165 37.931 87 52 2 418 502 24 110 2.05e-14 70.1
MS.gene40749.t1 AT2G43670 38.298 94 55 3 410 502 26 117 4.17e-14 69.7
MS.gene40749.t1 AT5G53600 39.535 86 50 2 418 502 27 111 5.32e-14 68.9
MS.gene40749.t1 AT2G04910 43.678 87 46 3 412 496 8 93 6.91e-14 69.3
MS.gene40749.t1 AT2G39640 34.091 88 57 1 419 505 459 546 8.49e-14 74.7
MS.gene40749.t1 AT2G39640 35.417 96 55 2 406 500 351 440 3.25e-12 69.7
MS.gene40749.t1 AT2G43660 41.667 96 52 4 409 502 26 119 1.34e-13 68.2
MS.gene40749.t1 AT2G43660 41.667 96 52 4 409 502 25 118 1.62e-13 68.2
MS.gene40749.t1 AT3G07320 39.216 102 58 3 405 504 358 457 3.74e-13 72.4
MS.gene40749.t1 AT2G29735 39.785 93 53 2 413 502 24 116 6.46e-13 66.2
MS.gene40749.t1 AT4G14080 37.255 102 62 2 405 504 374 475 7.22e-13 71.6
MS.gene40749.t1 AT1G13830 39.130 92 47 3 417 502 32 120 8.62e-13 68.6
MS.gene40749.t1 AT1G13830 39.326 89 45 3 420 502 21 106 1.28e-12 67.4
MS.gene40749.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.50e-12 65.1
MS.gene40749.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene40749.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene40749.t1 AT2G03505 36.667 90 54 2 419 505 20 109 1.77e-12 66.2
MS.gene40749.t1 AT5G53610 41.463 82 46 2 420 500 28 108 2.29e-12 64.3
MS.gene40749.t1 AT5G64790 38.144 97 56 4 418 512 367 461 5.64e-12 68.9

Find 141 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTACAATGCCAGATTT+TGG 0.194586 1.2:-59020632 MS.gene40749:CDS
CCAACATCTTCAGGCACTTT+TGG 0.216484 1.2:-59020802 MS.gene40749:CDS
TCACTTTCATGCAGGTTCTT+TGG 0.248475 1.2:-59022527 MS.gene40749:intron
TCTCTCTTAATTTCATGTTC+TGG 0.269637 1.2:-59022623 MS.gene40749:intron
TAGATTACTTTCATTTATAA+AGG 0.277901 1.2:-59022071 MS.gene40749:CDS
GGTGGAGCTGTTGGAATTGA+TGG 0.308099 1.2:+59020823 None:intergenic
AAGACTATACAGCCAGTAAA+AGG 0.355621 1.2:+59020551 None:intergenic
GGAGGCGCTGCTATGTTAGT+TGG 0.360601 1.2:-59021122 MS.gene40749:CDS
ATGAAGAAACTTTGCTCTGC+TGG 0.376113 1.2:+59022458 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.377508 1.2:+59021419 None:intergenic
ATGATAGAAGATAGCATTGA+TGG 0.377602 1.2:-59022031 MS.gene40749:CDS
CCTCCAAAGTCGCAACTTGT+TGG 0.380624 1.2:+59021140 None:intergenic
GGTGTAATGGTTGGAGTTGT+TGG 0.382964 1.2:+59021515 None:intergenic
GGTGGAGGTGTTGTTAGATT+TGG 0.383669 1.2:+59020889 None:intergenic
GGCATTGCAGCTGGATTTGT+AGG 0.384777 1.2:+59021578 None:intergenic
TGTGGAAGGAACTGCTATAA+TGG 0.388341 1.2:+59021619 None:intergenic
AACTCAAGCAACCCAGCTTC+AGG 0.391337 1.2:-59020650 MS.gene40749:CDS
GCTCCAACAAGTTGCGACTT+TGG 0.392668 1.2:-59021143 MS.gene40749:CDS
ATAACTGGTGTTGGATTTGT+AGG 0.394170 1.2:+59021662 None:intergenic
GCATGGTTCTGCAAAGTAAC+AGG 0.394899 1.2:+59021203 None:intergenic
GAGCTTTCGTCACAGATCAT+AGG 0.396108 1.2:-59022426 MS.gene40749:CDS
GGAGGTGTTGTTAGATTTGG+TGG 0.400113 1.2:+59020892 None:intergenic
CGAGTTACTTCTATTGAACT+TGG 0.401043 1.2:+59021890 None:intergenic
TTGGATTATGCTTGTGGAAA+TGG 0.401736 1.2:-59021269 MS.gene40749:CDS
CTTCAATGAAAGAACTCAAA+AGG 0.413209 1.2:-59021787 MS.gene40749:CDS
GTAGTTGAAGGAACTGTTAC+TGG 0.413784 1.2:+59021557 None:intergenic
GAAGGAGCATTTGTGTTTGA+AGG 0.413852 1.2:+59021356 None:intergenic
GTTGGAGTTGTTGGAGGTAA+AGG 0.413921 1.2:+59021524 None:intergenic
TTGAAGGAGGTGGCTGTTGA+GGG 0.417203 1.2:+59021372 None:intergenic
CACAAATGCTCCTTCTGCTC+AGG 0.420750 1.2:-59021348 MS.gene40749:CDS
ACAGCTCCACCAACATCTTC+AGG 0.424830 1.2:-59020811 MS.gene40749:CDS
ACCACTGGAACATTTCCTAA+TGG 0.424925 1.2:+59021398 None:intergenic
GGATAAGATGTTACTGGAGG+TGG 0.426667 1.2:+59021425 None:intergenic
GCCGATGTTGTGTTTACAAT+AGG 0.430103 1.2:+59020596 None:intergenic
ACTGTTACTGGCATTGCAGC+TGG 0.430772 1.2:+59021569 None:intergenic
TGATTGTTCTCAAATACAAC+AGG 0.433295 1.2:-59021243 MS.gene40749:CDS
ACAACTCCTACAACAGTTCC+AGG 0.436358 1.2:-59021470 MS.gene40749:CDS
ACATCTTATCCACCTCCATT+AGG 0.436623 1.2:-59021413 MS.gene40749:CDS
CTTTCATGCAGGTTCTTTGG+TGG 0.439102 1.2:-59022524 MS.gene40749:intron
GGAAATTGTGACAGGATTTG+TGG 0.439347 1.2:+59021601 None:intergenic
GGAGCTATTGTTGGAATAGA+TGG 0.441593 1.2:+59020868 None:intergenic
GGGCTATCAGACCCAAAATC+TGG 0.444861 1.2:+59020620 None:intergenic
TTTCATGCAGGTTCTTTGGT+GGG 0.448012 1.2:-59022523 MS.gene40749:intron
TTTGAAGGAGGTGGCTGTTG+AGG 0.448619 1.2:+59021371 None:intergenic
GGTGGAGCTGTTGACATTGT+TGG 0.450114 1.2:+59020844 None:intergenic
AGCTTCCCTTCAATCAGCAT+TGG 0.454325 1.2:-59021288 MS.gene40749:CDS
AACTTCCTCAAGACTTGAAG+TGG 0.458266 1.2:+59022121 None:intergenic
CTGAGGCTTGCAGTGACAAA+TGG 0.467298 1.2:+59020521 None:intergenic
ATTGTTGGTGGAGCTATTGT+TGG 0.472956 1.2:+59020859 None:intergenic
AGTTGGTGTGTGGCGAAAAC+AGG 0.475765 1.2:-59021320 MS.gene40749:CDS
AAGTCGCAACTTGTTGGAGC+TGG 0.478203 1.2:+59021146 None:intergenic
GAGGCAGAAAGAAGCTTCCT+TGG 0.478234 1.2:+59022658 None:intergenic
TCTGGCATTGTACCTGAAGC+TGG 0.478959 1.2:+59020638 None:intergenic
GGTGCACCTGGAACTGTTGT+AGG 0.485248 1.2:+59021464 None:intergenic
GTAGGGTAAACTGTGTTTGT+TGG 0.486254 1.2:+59021680 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.487201 1.2:+59021440 None:intergenic
GGCTGTTGAGGGTTTACCAC+TGG 0.491135 1.2:+59021383 None:intergenic
CATAATCCAATGCTGATTGA+AGG 0.493090 1.2:+59021282 None:intergenic
GTTGATTGTGTAGGTGGTGT+TGG 0.494210 1.2:+59020931 None:intergenic
TTTGTAGGGGAAATTGTGAC+AGG 0.494725 1.2:+59021593 None:intergenic
TATCTAGAATATTTCTTCTG+TGG 0.496929 1.2:+59021714 None:intergenic
AGTGTCTCCTCTCTCATGAT+AGG 0.500543 1.2:+59022495 None:intergenic
GTATCTGAGGGTGTAATAAC+TGG 0.502389 1.2:+59021647 None:intergenic
GCATTGCAGCTGGATTTGTA+GGG 0.503176 1.2:+59021579 None:intergenic
GATGTTACTGGTGGTGCACC+TGG 0.511282 1.2:+59021452 None:intergenic
TTCAATGAAAGAACTCAAAA+GGG 0.511868 1.2:-59021786 MS.gene40749:CDS
CTTGAGTTTAACACTGAAGG+TGG 0.514620 1.2:+59020665 None:intergenic
TCAGCATTGGATTATGCTTG+TGG 0.514748 1.2:-59021275 MS.gene40749:CDS
GCATTTGTGTTTGAAGGAGG+TGG 0.517825 1.2:+59021362 None:intergenic
CTCTTGATTGTAAGAACCAC+AGG 0.520516 1.2:+59021923 None:intergenic
CATTTCCACTTCAAGTCTTG+AGG 0.520651 1.2:-59022126 MS.gene40749:CDS
GGAGCATTTGTGTTTGAAGG+AGG 0.521515 1.2:+59021359 None:intergenic
GAAGACTGGTGCCGGTGTAA+TGG 0.528319 1.2:+59021502 None:intergenic
GATGTTGTGTTTACAATAGG+AGG 0.530245 1.2:+59020599 None:intergenic
CAACTCTGTCCCTGAGCAGA+AGG 0.531643 1.2:+59021338 None:intergenic
TCCTTGCAAAGATGTTCCTG+TGG 0.532621 1.2:-59021939 MS.gene40749:CDS
CCAACAAGTTGCGACTTTGG+AGG 0.533263 1.2:-59021140 MS.gene40749:CDS
GAAGATGTTGGTGGAGCTGT+TGG 0.534788 1.2:+59020814 None:intergenic
GGAACAGCAGGTGTAGTTGA+AGG 0.536488 1.2:+59021545 None:intergenic
GAAAGAACTCAAAAGGGAAG+AGG 0.546037 1.2:-59021780 MS.gene40749:CDS
GGAACATTTCCTAATGGAGG+TGG 0.552963 1.2:+59021404 None:intergenic
GTTGGAGGTAAAGGAACAGC+AGG 0.553488 1.2:+59021533 None:intergenic
TCTTACAAACTCAAATGTGT+TGG 0.554054 1.2:-59022392 MS.gene40749:CDS
GGAGCTGTTGACATTGTTGG+TGG 0.559801 1.2:+59020847 None:intergenic
GAGGGTGTAATAACTGGTGT+TGG 0.563477 1.2:+59021653 None:intergenic
ATAATCCAATGCTGATTGAA+GGG 0.564193 1.2:+59021283 None:intergenic
TTTAACTCGGAAACTGCTGA+AGG 0.564291 1.2:+59022319 None:intergenic
ACTGTTGTAGGAGTTGTAGC+TGG 0.566759 1.2:+59021476 None:intergenic
GGTGTTGTTAGATTTGGTGG+TGG 0.572595 1.2:+59020895 None:intergenic
TTGAGTTTAACACTGAAGGT+GGG 0.573230 1.2:+59020666 None:intergenic
TCTCTACTTAAACAAAAGCC+AGG 0.575198 1.2:-59022365 MS.gene40749:CDS
TAACTGGTGTTGGATTTGTA+GGG 0.580532 1.2:+59021663 None:intergenic
CTTCTGCTCAGGGACAGAGT+TGG 0.581714 1.2:-59021337 MS.gene40749:CDS
TTGCTTGAGTTTAACACTGA+AGG 0.581994 1.2:+59020662 None:intergenic
TTAAGAGCATGCATAATCAA+AGG 0.588835 1.2:+59022208 None:intergenic
TTTCAGAGCTCGAGGGTGCG+AGG 0.590599 1.2:+59020574 None:intergenic
TATAATGGTTGGAGTATCTG+AGG 0.591925 1.2:+59021634 None:intergenic
GAAGGAACTGCTATAATGGT+TGG 0.591976 1.2:+59021623 None:intergenic
AAGATCTCTCTTCTAGACAA+AGG 0.594620 1.2:+59021746 None:intergenic
GGTGTTGGAGATGATATCAT+TGG 0.598845 1.2:+59020946 None:intergenic
ATACTCTCTGATAATGGCCA+AGG 0.605015 1.2:-59022675 None:intergenic
GTAATGGTTGGAGTTGTTGG+AGG 0.606112 1.2:+59021518 None:intergenic
ATCATGAGAGAGGAGACACT+TGG 0.607322 1.2:-59022492 MS.gene40749:CDS
GTAAAAGGTTTCAGAGCTCG+AGG 0.607544 1.2:+59020566 None:intergenic
TGAGTTTAACACTGAAGGTG+GGG 0.608299 1.2:+59020667 None:intergenic
GAAGTTGAAGTTGATTGTGT+AGG 0.609053 1.2:+59020922 None:intergenic
TGATATCATTGGTGAAGTTG+AGG 0.609455 1.2:+59020957 None:intergenic
GTTGAAGTTGATTGTGTAGG+TGG 0.611559 1.2:+59020925 None:intergenic
ATGTTGTGTTTACAATAGGA+GGG 0.618672 1.2:+59020600 None:intergenic
GTTGTAGCTGGATTGAAGAC+TGG 0.620005 1.2:+59021488 None:intergenic
ACAAATGCTCCTTCTGCTCA+GGG 0.620260 1.2:-59021347 MS.gene40749:CDS
ATTGTGACAGGATTTGTGGA+AGG 0.620979 1.2:+59021605 None:intergenic
TTAACTCGGAAACTGCTGAA+GGG 0.622484 1.2:+59022320 None:intergenic
AGAGAGAAGAGAGAAACAAG+AGG 0.623621 1.2:+59022639 None:intergenic
GCTGGATTGAAGACTGGTGC+CGG 0.624496 1.2:+59021494 None:intergenic
AACAACTCCAACCATTACAC+CGG 0.624559 1.2:-59021513 MS.gene40749:CDS
GGAGCTGTTGGAATTGATGG+TGG 0.624833 1.2:+59020826 None:intergenic
ACTGGTGCCGGTGTAATGGT+TGG 0.625957 1.2:+59021506 None:intergenic
CTGGCATTGTACCTGAAGCT+GGG 0.626526 1.2:+59020639 None:intergenic
CAGTGATCTTCACATAAGCA+AGG 0.629071 1.2:-59022167 MS.gene40749:CDS
ACCACAGGAACATCTTTGCA+AGG 0.631919 1.2:+59021938 None:intergenic
AACTCTATAGGTGCATGCTG+AGG 0.632489 1.2:+59020504 None:intergenic
CATTGCAGCTGGATTTGTAG+GGG 0.635434 1.2:+59021580 None:intergenic
GAACTCAAAAGGGAAGAGGA+AGG 0.639773 1.2:-59021776 MS.gene40749:CDS
CCAAAAGTGCCTGAAGATGT+TGG 0.642640 1.2:+59020802 None:intergenic
TGTTGAAGGCAAAAGAAGCA+TGG 0.645483 1.2:+59021186 None:intergenic
GCTATTGTTGGAATAGATGG+TGG 0.647261 1.2:+59020871 None:intergenic
TCCATTAGGAAATGTTCCAG+TGG 0.658091 1.2:-59021399 MS.gene40749:CDS
ACTGGAACATTTCCTAATGG+AGG 0.658231 1.2:+59021401 None:intergenic
GCTGATTGAAGGGAAGCTTG+TGG 0.661755 1.2:+59021293 None:intergenic
TAAAAGGTTTCAGAGCTCGA+GGG 0.668012 1.2:+59020567 None:intergenic
ATTGTTGGAATAGATGGTGG+AGG 0.673696 1.2:+59020874 None:intergenic
TCCTATTGTAAACACAACAT+CGG 0.678848 1.2:-59020597 MS.gene40749:CDS
CAGAAGCAGCAAGATTAGCG+CGG 0.682382 1.2:+59021966 None:intergenic
AAAGTGCCTGAAGATGTTGG+TGG 0.686382 1.2:+59020805 None:intergenic
ATAATGGTTGGAGTATCTGA+GGG 0.698374 1.2:+59021635 None:intergenic
TCAGGGACAGAGTTGGTGTG+TGG 0.715688 1.2:-59021330 MS.gene40749:CDS
GGTGGATAAGATGTTACTGG+AGG 0.717889 1.2:+59021422 None:intergenic
GATTGTTCTCAAATACAACA+GGG 0.719905 1.2:-59021242 MS.gene40749:CDS
GACGACGATGAAGATGATGA+CGG 0.723295 1.2:+59020985 None:intergenic
GGTGGATAAGATGTTACTGG+TGG 0.740803 1.2:+59021443 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATTACTTTCATTTATAA+AGG - chr1.2:59021099-59021118 MS.gene40749:intron 15.0%
!! TTAAATGTTAAAATCTTACT+TGG + chr1.2:59022081-59022100 None:intergenic 15.0%
!!! TATTGATGATCATTTTTAAT+AGG - chr1.2:59022143-59022162 MS.gene40749:CDS 15.0%
!! ATTGAAAGAAAGAAAAAACA+AGG + chr1.2:59022109-59022128 None:intergenic 20.0%
!! TTGAAAGAAAGAAAAAACAA+GGG + chr1.2:59022108-59022127 None:intergenic 20.0%
!!! ATGTTTTTGATGTTTAAATG+TGG + chr1.2:59020899-59020918 None:intergenic 20.0%
!!! TCTTTTTGCTTCTAAATATT+TGG + chr1.2:59021323-59021342 None:intergenic 20.0%
! ATATTATTTCACTTTCATGC+AGG - chr1.2:59020635-59020654 MS.gene40749:CDS 25.0%
! TTCAATGAAAGAACTCAAAA+GGG - chr1.2:59021384-59021403 MS.gene40749:CDS 25.0%
!! TATCTAGAATATTTCTTCTG+TGG + chr1.2:59021459-59021478 None:intergenic 25.0%
!!! AAGTAATGAATTTTTCAACC+TGG + chr1.2:59020826-59020845 None:intergenic 25.0%
!!! TGATCATTTTTAATAGGTTC+TGG - chr1.2:59022149-59022168 MS.gene40749:CDS 25.0%
AAGCAAGGAAGTAAAAATCT+CGG - chr1.2:59021018-59021037 MS.gene40749:intron 30.0%
ATGTTGTGTTTACAATAGGA+GGG + chr1.2:59022573-59022592 None:intergenic 30.0%
CAATAAGCATGTACTAATCA+TGG - chr1.2:59022443-59022462 MS.gene40749:CDS 30.0%
CATGATTAGTACATGCTTAT+TGG + chr1.2:59022445-59022464 None:intergenic 30.0%
CTTCAATGAAAGAACTCAAA+AGG - chr1.2:59021383-59021402 MS.gene40749:CDS 30.0%
GATTGTTCTCAAATACAACA+GGG - chr1.2:59021928-59021947 MS.gene40749:CDS 30.0%
TCCTATTGTAAACACAACAT+CGG - chr1.2:59022573-59022592 MS.gene40749:intron 30.0%
TCTCTCTTAATTTCATGTTC+TGG - chr1.2:59020547-59020566 MS.gene40749:CDS 30.0%
TCTTACAAACTCAAATGTGT+TGG - chr1.2:59020778-59020797 MS.gene40749:intron 30.0%
TGATTGTTCTCAAATACAAC+AGG - chr1.2:59021927-59021946 MS.gene40749:CDS 30.0%
! ATAATCCAATGCTGATTGAA+GGG + chr1.2:59021890-59021909 None:intergenic 30.0%
! ATGATAGAAGATAGCATTGA+TGG - chr1.2:59021139-59021158 MS.gene40749:CDS 30.0%
! TTAAGAGCATGCATAATCAA+AGG + chr1.2:59020965-59020984 None:intergenic 30.0%
!! CTTTTGATAGTAACTGTTGA+AGG + chr1.2:59022001-59022020 None:intergenic 30.0%
AAAAACATCATTGCTAGCTG+TGG - chr1.2:59020911-59020930 MS.gene40749:CDS 35.0%
AAGACTATACAGCCAGTAAA+AGG + chr1.2:59022622-59022641 None:intergenic 35.0%
AAGATCTCTCTTCTAGACAA+AGG + chr1.2:59021427-59021446 None:intergenic 35.0%
CGAGTTACTTCTATTGAACT+TGG + chr1.2:59021283-59021302 None:intergenic 35.0%
GATGTTGTGTTTACAATAGG+AGG + chr1.2:59022574-59022593 None:intergenic 35.0%
TCTCTACTTAAACAAAAGCC+AGG - chr1.2:59020805-59020824 MS.gene40749:CDS 35.0%
TGATATCATTGGTGAAGTTG+AGG + chr1.2:59022216-59022235 None:intergenic 35.0%
TTGAGTTTAACACTGAAGGT+GGG + chr1.2:59022507-59022526 None:intergenic 35.0%
TTGCTTGAGTTTAACACTGA+AGG + chr1.2:59022511-59022530 None:intergenic 35.0%
TTGGATTATGCTTGTGGAAA+TGG - chr1.2:59021901-59021920 MS.gene40749:CDS 35.0%
! AATGCTGTCTTTTGTAGCTA+TGG - chr1.2:59022478-59022497 MS.gene40749:CDS 35.0%
! ATAACTGGTGTTGGATTTGT+AGG + chr1.2:59021511-59021530 None:intergenic 35.0%
! ATAATGGTTGGAGTATCTGA+GGG + chr1.2:59021538-59021557 None:intergenic 35.0%
! CATAATCCAATGCTGATTGA+AGG + chr1.2:59021891-59021910 None:intergenic 35.0%
! GAAGTTGAAGTTGATTGTGT+AGG + chr1.2:59022251-59022270 None:intergenic 35.0%
! TAACTGGTGTTGGATTTGTA+GGG + chr1.2:59021510-59021529 None:intergenic 35.0%
! TATAATGGTTGGAGTATCTG+AGG + chr1.2:59021539-59021558 None:intergenic 35.0%
!! CACTAGTTGAGCTTTTAACT+CGG + chr1.2:59020867-59020886 None:intergenic 35.0%
AACAACTCCAACCATTACAC+CGG - chr1.2:59021657-59021676 MS.gene40749:CDS 40.0%
AACTTCCTCAAGACTTGAAG+TGG + chr1.2:59021052-59021071 None:intergenic 40.0%
ACATCTTATCCACCTCCATT+AGG - chr1.2:59021757-59021776 MS.gene40749:CDS 40.0%
ACCACTGGAACATTTCCTAA+TGG + chr1.2:59021775-59021794 None:intergenic 40.0%
ACTGGAACATTTCCTAATGG+AGG + chr1.2:59021772-59021791 None:intergenic 40.0%
AGAGAGAAGAGAGAAACAAG+AGG + chr1.2:59020534-59020553 None:intergenic 40.0%
ATGAAGAAACTTTGCTCTGC+TGG + chr1.2:59020715-59020734 None:intergenic 40.0%
ATTGTGACAGGATTTGTGGA+AGG + chr1.2:59021568-59021587 None:intergenic 40.0%
CAGTGATCTTCACATAAGCA+AGG - chr1.2:59021003-59021022 MS.gene40749:CDS 40.0%
CATTTCCACTTCAAGTCTTG+AGG - chr1.2:59021044-59021063 MS.gene40749:intron 40.0%
CTCTTGATTGTAAGAACCAC+AGG + chr1.2:59021250-59021269 None:intergenic 40.0%
CTTGAGTTTAACACTGAAGG+TGG + chr1.2:59022508-59022527 None:intergenic 40.0%
GAAAGAACTCAAAAGGGAAG+AGG - chr1.2:59021390-59021409 MS.gene40749:CDS 40.0%
GAAGGAACTGCTATAATGGT+TGG + chr1.2:59021550-59021569 None:intergenic 40.0%
GCCGATGTTGTGTTTACAAT+AGG + chr1.2:59022577-59022596 None:intergenic 40.0%
GCTATTGTTGGAATAGATGG+TGG + chr1.2:59022302-59022321 None:intergenic 40.0%
GGAAATTGTGACAGGATTTG+TGG + chr1.2:59021572-59021591 None:intergenic 40.0%
GGAGCTATTGTTGGAATAGA+TGG + chr1.2:59022305-59022324 None:intergenic 40.0%
GTAGGGTAAACTGTGTTTGT+TGG + chr1.2:59021493-59021512 None:intergenic 40.0%
GTAGTTGAAGGAACTGTTAC+TGG + chr1.2:59021616-59021635 None:intergenic 40.0%
TAAAAGGTTTCAGAGCTCGA+GGG + chr1.2:59022606-59022625 None:intergenic 40.0%
TCACTTTCATGCAGGTTCTT+TGG - chr1.2:59020643-59020662 MS.gene40749:CDS 40.0%
TCCATTAGGAAATGTTCCAG+TGG - chr1.2:59021771-59021790 MS.gene40749:CDS 40.0%
TGAGTTTAACACTGAAGGTG+GGG + chr1.2:59022506-59022525 None:intergenic 40.0%
TGTGGAAGGAACTGCTATAA+TGG + chr1.2:59021554-59021573 None:intergenic 40.0%
TGTTGAAGGCAAAAGAAGCA+TGG + chr1.2:59021987-59022006 None:intergenic 40.0%
TTAACTCGGAAACTGCTGAA+GGG + chr1.2:59020853-59020872 None:intergenic 40.0%
TTTAACTCGGAAACTGCTGA+AGG + chr1.2:59020854-59020873 None:intergenic 40.0%
TTTCATGCAGGTTCTTTGGT+GGG - chr1.2:59020647-59020666 MS.gene40749:CDS 40.0%
TTTGTAGGGGAAATTGTGAC+AGG + chr1.2:59021580-59021599 None:intergenic 40.0%
! ATTGTTGGAATAGATGGTGG+AGG + chr1.2:59022299-59022318 None:intergenic 40.0%
! ATTGTTGGTGGAGCTATTGT+TGG + chr1.2:59022314-59022333 None:intergenic 40.0%
! CAACATCTTCAGGCACTTTT+GGG - chr1.2:59022369-59022388 MS.gene40749:CDS 40.0%
! CAGGTACAATGCCAGATTTT+GGG - chr1.2:59022539-59022558 MS.gene40749:intron 40.0%
! GGTGTTGGAGATGATATCAT+TGG + chr1.2:59022227-59022246 None:intergenic 40.0%
! GGTTTTTCCTATCATGAGAG+AGG - chr1.2:59020668-59020687 MS.gene40749:CDS 40.0%
! GTATCTGAGGGTGTAATAAC+TGG + chr1.2:59021526-59021545 None:intergenic 40.0%
! GTTGAAGTTGATTGTGTAGG+TGG + chr1.2:59022248-59022267 None:intergenic 40.0%
! TCAGCATTGGATTATGCTTG+TGG - chr1.2:59021895-59021914 MS.gene40749:CDS 40.0%
! TCAGGTACAATGCCAGATTT+TGG - chr1.2:59022538-59022557 MS.gene40749:intron 40.0%
!! GAAGGAGCATTTGTGTTTGA+AGG + chr1.2:59021817-59021836 None:intergenic 40.0%
ACAAATGCTCCTTCTGCTCA+GGG - chr1.2:59021823-59021842 MS.gene40749:CDS 45.0%
ACAACTCCTACAACAGTTCC+AGG - chr1.2:59021700-59021719 MS.gene40749:CDS 45.0%
ACCACAGGAACATCTTTGCA+AGG + chr1.2:59021235-59021254 None:intergenic 45.0%
ACTGTTGTAGGAGTTGTAGC+TGG + chr1.2:59021697-59021716 None:intergenic 45.0%
AGCTTCCCTTCAATCAGCAT+TGG - chr1.2:59021882-59021901 MS.gene40749:CDS 45.0%
AGTGTCTCCTCTCTCATGAT+AGG + chr1.2:59020678-59020697 None:intergenic 45.0%
ATCATGAGAGAGGAGACACT+TGG - chr1.2:59020678-59020697 MS.gene40749:CDS 45.0%
CATTGCAGCTGGATTTGTAG+GGG + chr1.2:59021593-59021612 None:intergenic 45.0%
CTTTCATGCAGGTTCTTTGG+TGG - chr1.2:59020646-59020665 MS.gene40749:CDS 45.0%
GAACTCAAAAGGGAAGAGGA+AGG - chr1.2:59021394-59021413 MS.gene40749:CDS 45.0%
GACGACGATGAAGATGATGA+CGG + chr1.2:59022188-59022207 None:intergenic 45.0%
GAGCTTTCGTCACAGATCAT+AGG - chr1.2:59020744-59020763 MS.gene40749:intron 45.0%
GCATTGCAGCTGGATTTGTA+GGG + chr1.2:59021594-59021613 None:intergenic 45.0%
GCATTTGTGTTTGAAGGAGG+TGG + chr1.2:59021811-59021830 None:intergenic 45.0%
GGAACATTTCCTAATGGAGG+TGG + chr1.2:59021769-59021788 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG + chr1.2:59021733-59021752 None:intergenic 45.0%
GGATAAGATGTTACTGGAGG+TGG + chr1.2:59021748-59021767 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+AGG + chr1.2:59021730-59021749 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+TGG + chr1.2:59021730-59021749 None:intergenic 45.0%
GGTGTAATGGTTGGAGTTGT+TGG + chr1.2:59021658-59021677 None:intergenic 45.0%
GTAAAAGGTTTCAGAGCTCG+AGG + chr1.2:59022607-59022626 None:intergenic 45.0%
GTAATGGTTGGAGTTGTTGG+AGG + chr1.2:59021655-59021674 None:intergenic 45.0%
GTTGATTGTGTAGGTGGTGT+TGG + chr1.2:59022242-59022261 None:intergenic 45.0%
GTTGGAGTTGTTGGAGGTAA+AGG + chr1.2:59021649-59021668 None:intergenic 45.0%
GTTGTAGCTGGATTGAAGAC+TGG + chr1.2:59021685-59021704 None:intergenic 45.0%
TCCTTGCAAAGATGTTCCTG+TGG - chr1.2:59021231-59021250 MS.gene40749:CDS 45.0%
! AAAGTGCCTGAAGATGTTGG+TGG + chr1.2:59022368-59022387 None:intergenic 45.0%
! CCAACATCTTCAGGCACTTT+TGG - chr1.2:59022368-59022387 MS.gene40749:CDS 45.0%
! GAGGGTGTAATAACTGGTGT+TGG + chr1.2:59021520-59021539 None:intergenic 45.0%
! GCATGGTTCTGCAAAGTAAC+AGG + chr1.2:59021970-59021989 None:intergenic 45.0%
! GGAGCATTTGTGTTTGAAGG+AGG + chr1.2:59021814-59021833 None:intergenic 45.0%
!! CCAAAAGTGCCTGAAGATGT+TGG + chr1.2:59022371-59022390 None:intergenic 45.0%
!! GGAGGTGTTGTTAGATTTGG+TGG + chr1.2:59022281-59022300 None:intergenic 45.0%
!! GGTGGAGGTGTTGTTAGATT+TGG + chr1.2:59022284-59022303 None:intergenic 45.0%
!! GGTGTTGTTAGATTTGGTGG+TGG + chr1.2:59022278-59022297 None:intergenic 45.0%
!!! CGAGCTCTGAAACCTTTTAC+TGG - chr1.2:59022607-59022626 MS.gene40749:intron 45.0%
AACTCAAGCAACCCAGCTTC+AGG - chr1.2:59022520-59022539 MS.gene40749:intron 50.0%
ACAGCTCCACCAACATCTTC+AGG - chr1.2:59022359-59022378 MS.gene40749:CDS 50.0%
ACTGTTACTGGCATTGCAGC+TGG + chr1.2:59021604-59021623 None:intergenic 50.0%
CACAAATGCTCCTTCTGCTC+AGG - chr1.2:59021822-59021841 MS.gene40749:CDS 50.0%
CAGAAGCAGCAAGATTAGCG+CGG + chr1.2:59021207-59021226 None:intergenic 50.0%
CCAACAAGTTGCGACTTTGG+AGG - chr1.2:59022030-59022049 MS.gene40749:CDS 50.0%
CCTCCAAAGTCGCAACTTGT+TGG + chr1.2:59022033-59022052 None:intergenic 50.0%
CTGAGGCTTGCAGTGACAAA+TGG + chr1.2:59022652-59022671 None:intergenic 50.0%
CTGGCATTGTACCTGAAGCT+GGG + chr1.2:59022534-59022553 None:intergenic 50.0%
GAGGCAGAAAGAAGCTTCCT+TGG + chr1.2:59020515-59020534 None:intergenic 50.0%
GCTCCAACAAGTTGCGACTT+TGG - chr1.2:59022027-59022046 MS.gene40749:CDS 50.0%
GCTGATTGAAGGGAAGCTTG+TGG + chr1.2:59021880-59021899 None:intergenic 50.0%
GGAACAGCAGGTGTAGTTGA+AGG + chr1.2:59021628-59021647 None:intergenic 50.0%
GGAGCTGTTGACATTGTTGG+TGG + chr1.2:59022326-59022345 None:intergenic 50.0%
GGAGCTGTTGGAATTGATGG+TGG + chr1.2:59022347-59022366 None:intergenic 50.0%
GGCATTGCAGCTGGATTTGT+AGG + chr1.2:59021595-59021614 None:intergenic 50.0%
GGGCTATCAGACCCAAAATC+TGG + chr1.2:59022553-59022572 None:intergenic 50.0%
GGTGGAGCTGTTGACATTGT+TGG + chr1.2:59022329-59022348 None:intergenic 50.0%
GGTGGAGCTGTTGGAATTGA+TGG + chr1.2:59022350-59022369 None:intergenic 50.0%
GTTGGAGGTAAAGGAACAGC+AGG + chr1.2:59021640-59021659 None:intergenic 50.0%
TCTGGCATTGTACCTGAAGC+TGG + chr1.2:59022535-59022554 None:intergenic 50.0%
TTGAAGGAGGTGGCTGTTGA+GGG + chr1.2:59021801-59021820 None:intergenic 50.0%
TTTGAAGGAGGTGGCTGTTG+AGG + chr1.2:59021802-59021821 None:intergenic 50.0%
! AGTTGGTGTGTGGCGAAAAC+AGG - chr1.2:59021850-59021869 MS.gene40749:CDS 50.0%
! GAAGATGTTGGTGGAGCTGT+TGG + chr1.2:59022359-59022378 None:intergenic 50.0%
!! AAGTCGCAACTTGTTGGAGC+TGG + chr1.2:59022027-59022046 None:intergenic 50.0%
!! GGCACTTTTGGGTAAGTGAG+AGG - chr1.2:59022380-59022399 MS.gene40749:CDS 50.0%
CAACTCTGTCCCTGAGCAGA+AGG + chr1.2:59021835-59021854 None:intergenic 55.0%
CTTCTGCTCAGGGACAGAGT+TGG - chr1.2:59021833-59021852 MS.gene40749:CDS 55.0%
GCTGGATTGAAGACTGGTGC+CGG + chr1.2:59021679-59021698 None:intergenic 55.0%
GGCTGTTGAGGGTTTACCAC+TGG + chr1.2:59021790-59021809 None:intergenic 55.0%
GGTGCACCTGGAACTGTTGT+AGG + chr1.2:59021709-59021728 None:intergenic 55.0%
! GATGTTACTGGTGGTGCACC+TGG + chr1.2:59021721-59021740 None:intergenic 55.0%
!! ACTGGTGCCGGTGTAATGGT+TGG + chr1.2:59021667-59021686 None:intergenic 55.0%
!! GAAGACTGGTGCCGGTGTAA+TGG + chr1.2:59021671-59021690 None:intergenic 55.0%
!! GGAGGCGCTGCTATGTTAGT+TGG - chr1.2:59022048-59022067 MS.gene40749:CDS 55.0%
!! TCAGGGACAGAGTTGGTGTG+TGG - chr1.2:59021840-59021859 MS.gene40749:CDS 55.0%
! TTTCAGAGCTCGAGGGTGCG+AGG + chr1.2:59022599-59022618 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 59020508 59022684 59020508 ID=MS.gene40749
chr1.2 mRNA 59020508 59022684 59020508 ID=MS.gene40749.t1;Parent=MS.gene40749
chr1.2 exon 59022624 59022684 59022624 ID=MS.gene40749.t1.exon1;Parent=MS.gene40749.t1
chr1.2 CDS 59022624 59022684 59022624 ID=cds.MS.gene40749.t1;Parent=MS.gene40749.t1
chr1.2 exon 59021111 59022535 59021111 ID=MS.gene40749.t1.exon2;Parent=MS.gene40749.t1
chr1.2 CDS 59021111 59022535 59021111 ID=cds.MS.gene40749.t1;Parent=MS.gene40749.t1
chr1.2 exon 59020802 59021027 59020802 ID=MS.gene40749.t1.exon3;Parent=MS.gene40749.t1
chr1.2 CDS 59020802 59021027 59020802 ID=cds.MS.gene40749.t1;Parent=MS.gene40749.t1
chr1.2 exon 59020508 59020697 59020508 ID=MS.gene40749.t1.exon4;Parent=MS.gene40749.t1
chr1.2 CDS 59020508 59020697 59020508 ID=cds.MS.gene40749.t1;Parent=MS.gene40749.t1
Gene Sequence

>MS.gene40749

ATGGCCAAGGAAGCTTCTTTCTGCCTCTTGTTTCTCTCTTCTCTCTTAATTTCATGTTCTGGTAAGTACTTTACACTGATAAATGATAACAACACATAGTACTCATTTTTGTGCAGATTCTAATCGTATATTATTTCACTTTCATGCAGGTTCTTTGGTGGGTTTTTCCTATCATGAGAGAGGAGACACTTGGACATCATTACTCCAGCAGAGCAAAGTTTCTTCATCTCAGATTCGAGCTTTCGTCACAGATCATAGGATTTTGAGCACTCTTACAAACTCAAATGTGTTGGTTGATCTCTACTTAAACAAAAGCCAGGTTGAAAAATTCATTACTTCAAACCCTTCAGCAGTTTCCGAGTTAAAAGCTCAACTAGTGAGCTTTCTTCCACATTTAAACATCAAAAACATCATTGCTAGCTGTGGAAGTGAGTGCTTAGTACAAAATGAGATACCTTTGATTATGCATGCTCTTAAATCAATTCATTCAATTCTCAGTGATCTTCACATAAGCAAGGAAGTAAAAATCTCGGTCGCATTTCCACTTCAAGTCTTGAGGAAGTTGAATGCATCACAAGAACACGAAATTCGTAGATTACTTTCATTTATAAAGGAAACGAAATCGTTTGTAATGATAGAAGATAGCATTGATGGAGAATTAAGTGTGAATGATCATTTTGTTCAAACAATTATCAACCGCGCTAATCTTGCTGCTTCTGTTCTTCCTTGCAAAGATGTTCCTGTGGTTCTTACAATCAAGAGTTCAGTTATACCAAGTTCAATAGAAGTAACTCGATTTAGTAAAAGAGTATCCAAATATTTAGAAGCAAAAAGAATAGCTGCATTATATGTAGAACTGCATACAACAGAAGATTCTTCAATGAAAGAACTCAAAAGGGAAGAGGAAGGTATGTTTCCTTTGTCTAGAAGAGAGATCTTAAGCAAATTCCACAGAAGAAATATTCTAGATAACACAAACAGTCCAACAAACACAGTTTACCCTACAAATCCAACACCAGTTATTACACCCTCAGATACTCCAACCATTATAGCAGTTCCTTCCACAAATCCTGTCACAATTTCCCCTACAAATCCAGCTGCAATGCCAGTAACAGTTCCTTCAACTACACCTGCTGTTCCTTTACCTCCAACAACTCCAACCATTACACCGGCACCAGTCTTCAATCCAGCTACAACTCCTACAACAGTTCCAGGTGCACCACCAGTAACATCTTATCCACCTCCAGTAACATCTTATCCACCTCCATTAGGAAATGTTCCAGTGGTAAACCCTCAACAGCCACCTCCTTCAAACACAAATGCTCCTTCTGCTCAGGGACAGAGTTGGTGTGTGGCGAAAACAGGTGCTCCACAAGCTTCCCTTCAATCAGCATTGGATTATGCTTGTGGAAATGGTGCTGATTGTTCTCAAATACAACAGGGTGCAAGTTGTTACAGTCCTGTTACTTTGCAGAACCATGCTTCTTTTGCCTTCAACAGTTACTATCAAAAGAAGCCAGCTCCAACAAGTTGCGACTTTGGAGGCGCTGCTATGTTAGTTGGCTCAAATCCAAGTAAGATTTTAACATTTAAATAACCCTTGTTTTTTCTTTCTTTCAATCTCATGCTTCAAAATATTGATGATCATTTTTAATAGGTTCTGGTTCTTGCATTTATCCGTCATCATCTTCATCGTCGTCGTCATCCTCAACTTCACCAATGATATCATCTCCAACACCACCTACACAATCAACTTCAACTTCAATACCACCACCAAATCTAACAACACCTCCACCATCTATTCCAACAATAGCTCCACCAACAATGTCAACAGCTCCACCATCAATTCCAACAGCTCCACCAACATCTTCAGGCACTTTTGGGTAAGTGAGAGGCAAGTTTTTCAGTGACTTCAATAATTATATTCTGTGATACCAATAAGCATGTACTAATCATGGTTCTGTATAAACAATGCTGTCTTTTGTAGCTATGGTACCCCACCTTCAGTGTTAAACTCAAGCAACCCAGCTTCAGGTACAATGCCAGATTTTGGGTCTGATAGCCCTCCTATTGTAAACACAACATCGGCCTCGCACCCTCGAGCTCTGAAACCTTTTACTGGCTGTATAGTCTTGATGATTCCATTTGTCACTGCAAGCCTCAGCATGCACCTATAG
Protein sequence

>MS.gene40749.t1

MAKEASFCLLFLSSLLISCSGSLVGFSYHERGDTWTSLLQQSKVSSSQIRAFVTDHRILSTLTNSNVLVDLYLNKSQVEKFITSNPSAVSELKAQLVSFLPHLNIKNIIASCGSECLVQNEIPLIMHALKSIHSILSDLHISKEVKISVAFPLQVLRKLNASQEHEIRRLLSFIKETKSFVMIEDSIDGELSVNDHFVQTIINRANLAASVLPCKDVPVVLTIKSSVIPSSIEVTRFSKRVSKYLEAKRIAALYVELHTTEDSSMKELKREEEGMFPLSRREILSKFHRRNILDNTNSPTNTVYPTNPTPVITPSDTPTIIAVPSTNPVTISPTNPAAMPVTVPSTTPAVPLPPTTPTITPAPVFNPATTPTTVPGAPPVTSYPPPVTSYPPPLGNVPVVNPQQPPPSNTNAPSAQGQSWCVAKTGAPQASLQSALDYACGNGADCSQIQQGASCYSPVTLQNHASFAFNSYYQKKPAPTSCDFGGAAMLVGSNPSSGSCIYPSSSSSSSSSSTSPMISSPTPPTQSTSTSIPPPNLTTPPPSIPTIAPPTMSTAPPSIPTAPPTSSGTFGYGTPPSVLNSSNPASGTMPDFGSDSPPIVNTTSASHPRALKPFTGCIVLMIPFVTASLSMHL