AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40798


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40798.t1 MTR_1g083950 100.000 165 0 0 1 165 1 165 2.71e-123 343
MS.gene40798.t1 MTR_1g087200 53.247 154 66 3 9 157 6 158 4.06e-56 174
MS.gene40798.t1 MTR_1g054750 40.741 162 83 3 10 158 23 184 2.52e-34 119
MS.gene40798.t1 MTR_1g054765 38.690 168 84 4 10 158 4 171 1.10e-32 114
MS.gene40798.t1 MTR_1g088640 40.132 152 88 2 11 159 14 165 2.85e-32 113
MS.gene40798.t1 MTR_1g088640 40.132 152 85 3 11 159 14 162 4.16e-31 110
MS.gene40798.t1 MTR_5g065980 37.107 159 86 4 11 155 10 168 3.79e-30 108
MS.gene40798.t1 MTR_5g065980 37.037 162 84 5 11 155 10 170 3.87e-30 108
MS.gene40798.t1 MTR_7g108830 40.123 162 90 4 1 158 1 159 4.98e-27 100
MS.gene40798.t1 MTR_8g058620 40.123 162 90 4 1 158 1 159 4.98e-27 100
MS.gene40798.t1 MTR_2g089350 30.539 167 102 4 9 163 25 189 1.39e-21 87.4
MS.gene40798.t1 MTR_5g077130 34.591 159 89 6 13 160 9 163 3.91e-19 79.7
MS.gene40798.t1 MTR_5g077130 33.962 159 89 5 13 160 9 162 8.50e-19 79.0
MS.gene40798.t1 MTR_1g088620 55.224 67 30 0 93 159 218 284 5.14e-18 79.0
MS.gene40798.t1 MTR_5g065980 32.374 139 80 4 11 135 10 148 8.26e-18 77.0
MS.gene40798.t1 MTR_2g034200 31.250 160 94 4 7 155 3 157 1.56e-17 75.5
MS.gene40798.t1 MTR_6g006190 27.485 171 110 5 6 164 3 171 4.19e-17 74.7
MS.gene40798.t1 MTR_4g019800 34.194 155 92 4 9 155 5 157 2.17e-16 72.4
MS.gene40798.t1 MTR_4g064933 31.902 163 101 4 7 161 3 163 4.80e-14 66.2
MS.gene40798.t1 MTR_6g006190 36.842 76 47 1 90 164 72 147 1.20e-12 62.4
MS.gene40798.t1 MTR_7g065770 31.613 155 96 4 9 155 5 157 1.61e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40798.t1 AT3G62550 50.993 151 71 1 9 156 7 157 5.62e-52 163
MS.gene40798.t1 AT3G11930 38.922 167 88 4 9 161 33 199 1.80e-34 120
MS.gene40798.t1 AT3G11930 39.157 166 88 4 9 161 33 198 2.04e-34 120
MS.gene40798.t1 AT3G11930 38.690 168 88 5 9 161 33 200 9.87e-34 118
MS.gene40798.t1 AT3G58450 38.650 163 88 4 11 161 32 194 7.65e-32 113
MS.gene40798.t1 AT3G58450 39.375 160 84 3 11 161 32 187 3.91e-31 111
MS.gene40798.t1 AT1G09740 37.654 162 91 3 11 162 10 171 1.21e-30 109
MS.gene40798.t1 AT1G09740 37.654 162 91 3 11 162 10 171 1.21e-30 109
MS.gene40798.t1 AT2G47710 36.875 160 92 4 5 158 3 159 6.37e-30 107
MS.gene40798.t1 AT3G11930 33.679 193 88 5 9 161 33 225 3.50e-28 104
MS.gene40798.t1 AT1G68300 36.242 149 92 3 9 155 10 157 5.29e-27 99.8
MS.gene40798.t1 AT3G17020 36.025 161 84 6 8 155 6 160 2.92e-20 82.4
MS.gene40798.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene40798.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene40798.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene40798.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene40798.t1 AT3G01520 31.944 144 93 4 24 164 30 171 1.52e-17 75.9
MS.gene40798.t1 AT5G14680 29.940 167 103 5 10 164 7 171 8.73e-17 73.9
MS.gene40798.t1 AT3G03270 30.818 159 94 5 8 155 4 157 1.99e-15 69.7
MS.gene40798.t1 AT3G53990 28.302 159 96 4 9 155 5 157 3.11e-12 61.6
MS.gene40798.t1 AT3G25930 29.012 162 87 8 9 156 2 149 3.16e-11 58.5

Find 44 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTCCAGTGGTTGATTT+TGG 0.145041 1.2:+59549592 None:intergenic
CACATGGTGCCTCAAGAATT+TGG 0.151045 1.2:-59550294 MS.gene40798:CDS
GTTGTTTATTCTGCCTTTGA+TGG 0.302678 1.2:-59550218 MS.gene40798:CDS
GGCTTCTTCACAATCAAAAC+AGG 0.346785 1.2:+59549613 None:intergenic
ACAAGTTGCTGATTGTGTTT+TGG 0.353665 1.2:-59550058 MS.gene40798:CDS
GGGTGTTGATATCTTGGTTA+TGG 0.361677 1.2:-59549832 MS.gene40798:CDS
GAGGAGGATAAGGTGATCTT+TGG 0.363644 1.2:+59550259 None:intergenic
GACATCATTACAGACTATCT+TGG 0.412460 1.2:+59550029 None:intergenic
GGTGTTGATATCTTGGTTAT+GGG 0.433422 1.2:-59549831 MS.gene40798:CDS
AAACACAATCAGCAACTTGT+TGG 0.448947 1.2:+59550061 None:intergenic
CCGTTATGTCAGAAGAGAAT+AGG 0.454640 1.2:+59550103 None:intergenic
CCCTAGGGATGTAATCTGTC+AGG 0.458933 1.2:-59549868 MS.gene40798:CDS
TATTCTGCCTTTGATGGCAC+AGG 0.479279 1.2:-59550212 MS.gene40798:intron
TTGAATGGCAGGGCTTTCCT+TGG 0.488782 1.2:-59549673 MS.gene40798:intron
ATCTTAATTGTCTCCTCCAG+TGG 0.492223 1.2:+59549582 None:intergenic
TTGTTGGCTATACTTCTCCA+TGG 0.495342 1.2:+59550077 None:intergenic
GCTTAACATAGAGGAGGATA+AGG 0.496830 1.2:+59550249 None:intergenic
ATGGCTGGTATAACAGAAAA+CGG 0.497259 1.2:-59550368 MS.gene40798:CDS
GTGGAGACGAGGATAGAGAA+TGG 0.517274 1.2:-59549894 MS.gene40798:CDS
GTTATGGGAAGCCACGGCTA+TGG 0.518096 1.2:-59549816 MS.gene40798:CDS
GGATAGAGAATGGTGACCCT+AGG 0.527492 1.2:-59549884 MS.gene40798:CDS
CCTATTCTCTTCTGACATAA+CGG 0.542181 1.2:-59550103 MS.gene40798:CDS
ATCAAGCAGTGTGAAATGGC+TGG 0.546216 1.2:-59550383 None:intergenic
ATCTTGGTTATGGGAAGCCA+CGG 0.562584 1.2:-59549822 MS.gene40798:CDS
AGAAAACGGAAGAAGAATCA+TGG 0.569970 1.2:-59550354 MS.gene40798:CDS
CACGAGGAGGCTTAACATAG+AGG 0.577003 1.2:+59550240 None:intergenic
AGTGGTTGCTCACACTTCCA+AGG 0.579050 1.2:+59549656 None:intergenic
TTAATTACCTGTGCCATCAA+AGG 0.579397 1.2:+59550205 None:intergenic
GATAGAGAATGGTGACCCTA+GGG 0.580628 1.2:-59549883 MS.gene40798:CDS
AAGAAGCCAAAATCAACCAC+TGG 0.584975 1.2:-59549598 MS.gene40798:CDS
GCCTGACAGATTACATCCCT+AGG 0.585000 1.2:+59549867 None:intergenic
ATCATGGTAGCTATTGATGA+AGG 0.586206 1.2:-59550338 MS.gene40798:CDS
TTCTGACATAACGGCAACCA+TGG 0.586507 1.2:-59550094 MS.gene40798:CDS
ACGGCTATGGTGTCATCAAG+AGG 0.638531 1.2:-59549803 MS.gene40798:intron
AAGCCAAAATCAACCACTGG+AGG 0.644827 1.2:-59549595 MS.gene40798:CDS
GCTTCTTCACAATCAAAACA+GGG 0.652447 1.2:+59549614 None:intergenic
ATATGGTTTAGGTGGAGACG+AGG 0.658827 1.2:-59549905 MS.gene40798:intron
CCTGACAGATTACATCCCTA+GGG 0.659449 1.2:+59549868 None:intergenic
AGAGCATTTATGCTCTCACA+TGG 0.669047 1.2:-59550310 MS.gene40798:CDS
TCTTGATGACACCATAGCCG+TGG 0.679887 1.2:+59549805 None:intergenic
ACTTCACATTCTGTGCACAG+TGG 0.689187 1.2:+59549638 None:intergenic
GCAGAATAAACAACACGAGG+AGG 0.692643 1.2:+59550227 None:intergenic
GAGGAGGCTTAACATAGAGG+AGG 0.704073 1.2:+59550243 None:intergenic
AAGGCAGAATAAACAACACG+AGG 0.733599 1.2:+59550224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAACTATAGATAAAAATG+AGG + chr1.2:59550008-59550027 None:intergenic 15.0%
!! TGTGAATTGTAAATTTAATT+TGG - chr1.2:59550242-59550261 MS.gene40798:CDS 15.0%
!! TTAATAATTAATCTGTGATA+TGG - chr1.2:59550031-59550050 MS.gene40798:CDS 15.0%
!!! ATCAATGTATACTTACATTT+TGG + chr1.2:59549949-59549968 None:intergenic 20.0%
!!! TTGTAAATTTAATTTGGTTC+TGG - chr1.2:59550248-59550267 MS.gene40798:CDS 20.0%
! AACTATAGATAAAAATGAGG+AGG + chr1.2:59550005-59550024 None:intergenic 25.0%
! ACTATAGATAAAAATGAGGA+GGG + chr1.2:59550004-59550023 None:intergenic 25.0%
! ATTAATCTGTGATATGGTTT+AGG - chr1.2:59550037-59550056 MS.gene40798:CDS 25.0%
! GTATCATATATACATGTTTC+AGG - chr1.2:59549824-59549843 MS.gene40798:CDS 25.0%
! TTTGAAAAACCAAATTCTTG+AGG + chr1.2:59549671-59549690 None:intergenic 25.0%
!! ATTTTTGTTAGAATGAGCTA+AGG - chr1.2:59550218-59550237 MS.gene40798:CDS 25.0%
!! AAAAATGGGTGTTGATATCT+TGG - chr1.2:59550115-59550134 MS.gene40798:intron 30.0%
AAACACAATCAGCAACTTGT+TGG + chr1.2:59549895-59549914 None:intergenic 35.0%
AATCTGTGATATGGTTTAGG+TGG - chr1.2:59550040-59550059 MS.gene40798:CDS 35.0%
AGAAAACGGAAGAAGAATCA+TGG - chr1.2:59549599-59549618 MS.gene40798:CDS 35.0%
ATCATGGTAGCTATTGATGA+AGG - chr1.2:59549615-59549634 MS.gene40798:CDS 35.0%
ATGGCTGGTATAACAGAAAA+CGG - chr1.2:59549585-59549604 MS.gene40798:CDS 35.0%
CCTATTCTCTTCTGACATAA+CGG - chr1.2:59549850-59549869 MS.gene40798:CDS 35.0%
GACATCATTACAGACTATCT+TGG + chr1.2:59549927-59549946 None:intergenic 35.0%
GCTTCTTCACAATCAAAACA+GGG + chr1.2:59550342-59550361 None:intergenic 35.0%
GTTGTTTATTCTGCCTTTGA+TGG - chr1.2:59549735-59549754 MS.gene40798:intron 35.0%
TGTCAGGCTGTTCAAAAAAT+GGG - chr1.2:59550101-59550120 MS.gene40798:CDS 35.0%
TTAATTACCTGTGCCATCAA+AGG + chr1.2:59549751-59549770 None:intergenic 35.0%
! TTCTGGCTGTTATTGTTGAA+TGG - chr1.2:59550265-59550284 MS.gene40798:CDS 35.0%
!! ACAAGTTGCTGATTGTGTTT+TGG - chr1.2:59549895-59549914 MS.gene40798:CDS 35.0%
!! GGTGTTGATATCTTGGTTAT+GGG - chr1.2:59550122-59550141 MS.gene40798:intron 35.0%
AAGAAGCCAAAATCAACCAC+TGG - chr1.2:59550355-59550374 MS.gene40798:CDS 40.0%
AAGGCAGAATAAACAACACG+AGG + chr1.2:59549732-59549751 None:intergenic 40.0%
CCGTTATGTCAGAAGAGAAT+AGG + chr1.2:59549853-59549872 None:intergenic 40.0%
CTGTCAGGCTGTTCAAAAAA+TGG - chr1.2:59550100-59550119 MS.gene40798:CDS 40.0%
GCTGTTATTGTTGAATGGCA+GGG - chr1.2:59550270-59550289 MS.gene40798:CDS 40.0%
GCTTAACATAGAGGAGGATA+AGG + chr1.2:59549707-59549726 None:intergenic 40.0%
GGCTTCTTCACAATCAAAAC+AGG + chr1.2:59550343-59550362 None:intergenic 40.0%
! AGAGCATTTATGCTCTCACA+TGG - chr1.2:59549643-59549662 MS.gene40798:CDS 40.0%
! TTGTTGGCTATACTTCTCCA+TGG + chr1.2:59549879-59549898 None:intergenic 40.0%
!! GGGTGTTGATATCTTGGTTA+TGG - chr1.2:59550121-59550140 MS.gene40798:intron 40.0%
AAGCCAAAATCAACCACTGG+AGG - chr1.2:59550358-59550377 MS.gene40798:CDS 45.0%
ACTTCACATTCTGTGCACAG+TGG + chr1.2:59550318-59550337 None:intergenic 45.0%
ATATGGTTTAGGTGGAGACG+AGG - chr1.2:59550048-59550067 MS.gene40798:CDS 45.0%
ATCTTGGTTATGGGAAGCCA+CGG - chr1.2:59550131-59550150 MS.gene40798:intron 45.0%
CACATGGTGCCTCAAGAATT+TGG - chr1.2:59549659-59549678 MS.gene40798:CDS 45.0%
CCTGACAGATTACATCCCTA+GGG + chr1.2:59550088-59550107 None:intergenic 45.0%
GAGGAGGATAAGGTGATCTT+TGG + chr1.2:59549697-59549716 None:intergenic 45.0%
GATAGAGAATGGTGACCCTA+GGG - chr1.2:59550070-59550089 MS.gene40798:CDS 45.0%
GCAGAATAAACAACACGAGG+AGG + chr1.2:59549729-59549748 None:intergenic 45.0%
GGCTGTTATTGTTGAATGGC+AGG - chr1.2:59550269-59550288 MS.gene40798:CDS 45.0%
TTCTGACATAACGGCAACCA+TGG - chr1.2:59549859-59549878 MS.gene40798:CDS 45.0%
! TATTCTGCCTTTGATGGCAC+AGG - chr1.2:59549741-59549760 MS.gene40798:intron 45.0%
!! TCTCCTCCAGTGGTTGATTT+TGG + chr1.2:59550364-59550383 None:intergenic 45.0%
AGTGGTTGCTCACACTTCCA+AGG + chr1.2:59550300-59550319 None:intergenic 50.0%
CACGAGGAGGCTTAACATAG+AGG + chr1.2:59549716-59549735 None:intergenic 50.0%
CCCTAGGGATGTAATCTGTC+AGG - chr1.2:59550085-59550104 MS.gene40798:CDS 50.0%
GAGGAGGCTTAACATAGAGG+AGG + chr1.2:59549713-59549732 None:intergenic 50.0%
GCCTGACAGATTACATCCCT+AGG + chr1.2:59550089-59550108 None:intergenic 50.0%
GGATAGAGAATGGTGACCCT+AGG - chr1.2:59550069-59550088 MS.gene40798:CDS 50.0%
GTGGAGACGAGGATAGAGAA+TGG - chr1.2:59550059-59550078 MS.gene40798:CDS 50.0%
TTGAATGGCAGGGCTTTCCT+TGG - chr1.2:59550280-59550299 MS.gene40798:CDS 50.0%
! TCTTGATGACACCATAGCCG+TGG + chr1.2:59550151-59550170 None:intergenic 50.0%
!! ACGGCTATGGTGTCATCAAG+AGG - chr1.2:59550150-59550169 MS.gene40798:intron 50.0%
GTTATGGGAAGCCACGGCTA+TGG - chr1.2:59550137-59550156 MS.gene40798:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 59549585 59550390 59549585 ID=MS.gene40798
chr1.2 mRNA 59549585 59550390 59549585 ID=MS.gene40798.t1;Parent=MS.gene40798
chr1.2 exon 59550213 59550390 59550213 ID=MS.gene40798.t1.exon1;Parent=MS.gene40798.t1
chr1.2 CDS 59550213 59550390 59550213 ID=cds.MS.gene40798.t1;Parent=MS.gene40798.t1
chr1.2 exon 59550023 59550129 59550023 ID=MS.gene40798.t1.exon2;Parent=MS.gene40798.t1
chr1.2 CDS 59550023 59550129 59550023 ID=cds.MS.gene40798.t1;Parent=MS.gene40798.t1
chr1.2 exon 59549804 59549916 59549804 ID=MS.gene40798.t1.exon3;Parent=MS.gene40798.t1
chr1.2 CDS 59549804 59549916 59549804 ID=cds.MS.gene40798.t1;Parent=MS.gene40798.t1
chr1.2 exon 59549585 59549684 59549585 ID=MS.gene40798.t1.exon4;Parent=MS.gene40798.t1
chr1.2 CDS 59549585 59549684 59549585 ID=cds.MS.gene40798.t1;Parent=MS.gene40798.t1
Gene Sequence

>MS.gene40798

ATGGCTGGTATAACAGAAAACGGAAGAAGAATCATGGTAGCTATTGATGAAGGTGAAGAGAGCATTTATGCTCTCACATGGTGCCTCAAGAATTTGGTTTTTCAAAATTCCAAAGATCACCTTATCCTCCTCTATGTTAAGCCTCCTCGTGTTGTTTATTCTGCCTTTGATGGCACAGGTAATTAAACAAAATTTTATGATACGTAGCAAATAATTTTGTAAACTCATAATTAATTTTTGTATCATATATACATGTTTCAGGATACCTATTCTCTTCTGACATAACGGCAACCATGGAGAAGTATAGCCAACAAGTTGCTGATTGTGTTTTGGAGAAAGCCAAGATAGTCTGTAATGATGTCCAAAATGTAAGTATACATTGATCAATTGTTACTAGTTAGATATGTATATTATGTCCCTCCTCATTTTTATCTATAGTTAATTATTTAATAATTAATCTGTGATATGGTTTAGGTGGAGACGAGGATAGAGAATGGTGACCCTAGGGATGTAATCTGTCAGGCTGTTCAAAAAATGGGTGTTGATATCTTGGTTATGGGAAGCCACGGCTATGGTGTCATCAAGAGGTCAGTTTTTGATAATTCATACAATACATATTTGAATTGTAAAGTTATTTTTGTTAGAATGAGCTAAGGATGTGAATTGTAAATTTAATTTGGTTCTGGCTGTTATTGTTGAATGGCAGGGCTTTCCTTGGAAGTGTGAGCAACCACTGTGCACAGAATGTGAAGTGCCCTGTTTTGATTGTGAAGAAGCCAAAATCAACCACTGGAGGAGACAATTAA

Protein sequence

>MS.gene40798.t1

MAGITENGRRIMVAIDEGEESIYALTWCLKNLVFQNSKDHLILLYVKPPRVVYSAFDGTGYLFSSDITATMEKYSQQVADCVLEKAKIVCNDVQNVETRIENGDPRDVICQAVQKMGVDILVMGSHGYGVIKRAFLGSVSNHCAQNVKCPVLIVKKPKSTTGGDN