Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40834.t1 | XP_003591158.1 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 9.40E-61 | 242.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40834.t1 | Q9M9W1 | 84.8 | 125 | 18 | 1 | 1 | 125 | 1 | 124 | 8.0e-53 | 207.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40834.t1 | G7I8Q9 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 6.7e-61 | 242.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050252 | MS.gene40834 | 0.804064 | 2.58E-49 | -1.69E-46 |
| MS.gene051963 | MS.gene40834 | 0.817177 | 3.77E-52 | -1.69E-46 |
| MS.gene057052 | MS.gene40834 | 0.848943 | 4.44E-60 | -1.69E-46 |
| MS.gene057092 | MS.gene40834 | 0.833798 | 4.34E-56 | -1.69E-46 |
| MS.gene057235 | MS.gene40834 | 0.802293 | 6.01E-49 | -1.69E-46 |
| MS.gene058994 | MS.gene40834 | 0.840167 | 1.02E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40834.t1 | MTR_1g083430 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 2.92e-88 | 252 |
| MS.gene40834.t1 | MTR_1g088450 | 91.597 | 119 | 9 | 1 | 7 | 125 | 2 | 119 | 2.82e-65 | 194 |
| MS.gene40834.t1 | MTR_7g112770 | 94.444 | 108 | 6 | 0 | 18 | 125 | 17 | 124 | 5.56e-62 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40834.t1 | AT3G05560 | 84.800 | 125 | 18 | 1 | 1 | 125 | 1 | 124 | 3.71e-63 | 188 |
| MS.gene40834.t1 | AT3G05560 | 84.800 | 125 | 18 | 1 | 1 | 125 | 1 | 124 | 3.71e-63 | 188 |
| MS.gene40834.t1 | AT3G05560 | 84.800 | 125 | 18 | 1 | 1 | 125 | 1 | 124 | 3.71e-63 | 188 |
| MS.gene40834.t1 | AT5G27770 | 84.694 | 98 | 15 | 0 | 17 | 114 | 16 | 113 | 3.82e-49 | 153 |
| MS.gene40834.t1 | AT1G02830 | 69.492 | 118 | 33 | 2 | 1 | 116 | 1 | 117 | 7.80e-43 | 137 |
Find 27 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATGCTGTTACTGTTTCC+AGG | 0.305504 | 1.2:-59906081 | MS.gene40834:CDS |
| GATCTTGTCTTCCACGGGTT+TGG | 0.336765 | 1.2:+59906174 | None:intergenic |
| GTTTCTTCAAGAGAGGATTA+AGG | 0.367162 | 1.2:-59906128 | MS.gene40834:CDS |
| ACTTGAAGAAACACAATGTA+AGG | 0.413403 | 1.2:-59905078 | MS.gene40834:CDS |
| CACGGGTTTGGCACAGTCAA+TGG | 0.421170 | 1.2:+59906186 | None:intergenic |
| GGTGGTAAACCTGGTGCTCT+TGG | 0.432206 | 1.2:-59906103 | MS.gene40834:CDS |
| GTCCATGATCTTGTCTTCCA+CGG | 0.443029 | 1.2:+59906168 | None:intergenic |
| ATTAAGGTTGGTGGTAAACC+TGG | 0.503896 | 1.2:-59906112 | MS.gene40834:CDS |
| GTAGATGCAGCTGGTTCAAA+GGG | 0.506848 | 1.2:-59906235 | MS.gene40834:CDS |
| TGGTTCAAAGGGAAAGAAGA+AGG | 0.510234 | 1.2:-59906224 | MS.gene40834:CDS |
| CTGATAGCAACTTCTCCAAA+CGG | 0.520527 | 1.2:-59906033 | MS.gene40834:intron |
| AGAAACACAATGTAAGGGAT+TGG | 0.522187 | 1.2:-59905072 | MS.gene40834:CDS |
| TCCATGATCTTGTCTTCCAC+GGG | 0.522466 | 1.2:+59906169 | None:intergenic |
| TCGAAAAGTTTCTTCAAGAG+AGG | 0.523656 | 1.2:-59906135 | MS.gene40834:CDS |
| CAAGAGAGGATTAAGGTTGG+TGG | 0.536618 | 1.2:-59906121 | MS.gene40834:CDS |
| AGTAGATGCAGCTGGTTCAA+AGG | 0.536758 | 1.2:-59906236 | MS.gene40834:CDS |
| TTCAACATTGCTGAGAATGA+AGG | 0.551218 | 1.2:-59904998 | MS.gene40834:CDS |
| ACCCGTGGAAGACAAGATCA+TGG | 0.560910 | 1.2:-59906170 | MS.gene40834:CDS |
| CTTCAAGAGAGGATTAAGGT+TGG | 0.564429 | 1.2:-59906124 | MS.gene40834:CDS |
| ATGGGTCGAGTAGATGCAGC+TGG | 0.584016 | 1.2:-59906244 | MS.gene40834:CDS |
| ACAGCATCACCAAGAGCACC+AGG | 0.602480 | 1.2:+59906094 | None:intergenic |
| ATAGAAATGTTTATGAGCTG+AGG | 0.605170 | 1.2:-59905024 | MS.gene40834:CDS |
| TGATGCTGTTACTGTTTCCA+GGG | 0.614918 | 1.2:-59906080 | MS.gene40834:CDS |
| GGTTCAAAGGGAAAGAAGAA+GGG | 0.618619 | 1.2:-59906223 | MS.gene40834:CDS |
| CATTGACTGTGCCAAACCCG+TGG | 0.627134 | 1.2:-59906185 | MS.gene40834:CDS |
| CTTGAAGAAACACAATGTAA+GGG | 0.629064 | 1.2:-59905077 | MS.gene40834:CDS |
| CATTGCTGAGAATGAAGGAG+AGG | 0.630620 | 1.2:-59904993 | MS.gene40834:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GGATAATTTTTTTGAAAATA+AGG | + | chr1.2:59905927-59905946 | None:intergenic | 15.0% |
| !! | CCAAAAACTTCTAATATTAT+TGG | - | chr1.2:59905835-59905854 | MS.gene40834:intron | 20.0% |
| !!! | AGTGTTAAAATTGATTCTAA+AGG | + | chr1.2:59905528-59905547 | None:intergenic | 20.0% |
| !!! | AGTTTTTTGTTTTGTTTGAT+TGG | - | chr1.2:59905262-59905281 | MS.gene40834:intron | 20.0% |
| !!! | ATTTAGGTTGTTTTTTATGA+TGG | - | chr1.2:59905323-59905342 | MS.gene40834:intron | 20.0% |
| !!! | CCAATAATATTAGAAGTTTT+TGG | + | chr1.2:59905838-59905857 | None:intergenic | 20.0% |
| !!! | TGATTTTGTAAAACTAATGT+TGG | + | chr1.2:59905594-59905613 | None:intergenic | 20.0% |
| !!! | TTATGTTAACTTTTGATTCA+TGG | - | chr1.2:59905772-59905791 | MS.gene40834:intron | 20.0% |
| !! | ATATTATTGGCAATTTTGTG+TGG | - | chr1.2:59905848-59905867 | MS.gene40834:intron | 25.0% |
| !! | GGAATTCAATTGTTTGATTT+AGG | - | chr1.2:59905307-59905326 | MS.gene40834:intron | 25.0% |
| !! | TAAAATTGATTCTAAAGGTG+TGG | + | chr1.2:59905523-59905542 | None:intergenic | 25.0% |
| !! | TATTATTGGCAATTTTGTGT+GGG | - | chr1.2:59905849-59905868 | MS.gene40834:intron | 25.0% |
| !! | TTGATTTTACAATCTCGTTT+GGG | - | chr1.2:59905658-59905677 | MS.gene40834:intron | 25.0% |
| !!! | AATTTTAACACTACTAGAAG+TGG | - | chr1.2:59905536-59905555 | MS.gene40834:intron | 25.0% |
| !!! | ATTGATTTTACAATCTCGTT+TGG | - | chr1.2:59905657-59905676 | MS.gene40834:intron | 25.0% |
| !!! | TTTTTGTTTTGTTTGATTGG+AGG | - | chr1.2:59905265-59905284 | MS.gene40834:intron | 25.0% |
| AAGAATAGAGAATAACCGTT+TGG | + | chr1.2:59905211-59905230 | None:intergenic | 30.0% | |
| ACGAGTTGTTAAAAAAAGCA+TGG | + | chr1.2:59906092-59906111 | None:intergenic | 30.0% | |
| ACTTGAAGAAACACAATGTA+AGG | - | chr1.2:59906148-59906167 | MS.gene40834:CDS | 30.0% | |
| ATAGAAATGTTTATGAGCTG+AGG | - | chr1.2:59906202-59906221 | MS.gene40834:CDS | 30.0% | |
| CTTGAAGAAACACAATGTAA+GGG | - | chr1.2:59906149-59906168 | MS.gene40834:CDS | 30.0% | |
| GTAAAATCAATGTGTGTGTT+TGG | + | chr1.2:59905648-59905667 | None:intergenic | 30.0% | |
| TCATGGAATATACAATCTCT+GGG | - | chr1.2:59905789-59905808 | MS.gene40834:intron | 30.0% | |
| TCTGTCTAAATTGCAATGAT+GGG | - | chr1.2:59905879-59905898 | MS.gene40834:intron | 30.0% | |
| TTCATGGAATATACAATCTC+TGG | - | chr1.2:59905788-59905807 | MS.gene40834:intron | 30.0% | |
| TTCTGTCTAAATTGCAATGA+TGG | - | chr1.2:59905878-59905897 | MS.gene40834:intron | 30.0% | |
| ! | ACACAGTTTTTGTGATCTAA+AGG | - | chr1.2:59906062-59906081 | MS.gene40834:CDS | 30.0% |
| ! | ATTATTGGCAATTTTGTGTG+GGG | - | chr1.2:59905850-59905869 | MS.gene40834:intron | 30.0% |
| ! | GGATTTGTGTTTTCTTAGTT+AGG | - | chr1.2:59905286-59905305 | MS.gene40834:intron | 30.0% |
| ! | GTGTTTCTTCAAGTACTTTT+TGG | + | chr1.2:59906142-59906161 | None:intergenic | 30.0% |
| !!! | AATGTGTGTGTTTGGTTTTA+GGG | + | chr1.2:59905640-59905659 | None:intergenic | 30.0% |
| !!! | TGTTTTTTATGATGGTTCTG+CGG | - | chr1.2:59905331-59905350 | MS.gene40834:intron | 30.0% |
| AAATTATCCCCTGCTTCAAA+AGG | - | chr1.2:59905938-59905957 | MS.gene40834:intron | 35.0% | |
| AAGAGTGAATTGAAGATGGT+AGG | + | chr1.2:59905569-59905588 | None:intergenic | 35.0% | |
| AGAAACACAATGTAAGGGAT+TGG | - | chr1.2:59906154-59906173 | MS.gene40834:CDS | 35.0% | |
| CAATTGTTCGTATTCAGATG+GGG | - | chr1.2:59905974-59905993 | MS.gene40834:intron | 35.0% | |
| CATATTGCGATGATGCTAAA+AGG | + | chr1.2:59905713-59905732 | None:intergenic | 35.0% | |
| CCGATTAAAAGTGAGTTGTA+GGG | - | chr1.2:59905383-59905402 | MS.gene40834:intron | 35.0% | |
| GCAATTGTTCGTATTCAGAT+GGG | - | chr1.2:59905973-59905992 | MS.gene40834:intron | 35.0% | |
| GTTTCTTCAAGAGAGGATTA+AGG | - | chr1.2:59905098-59905117 | MS.gene40834:CDS | 35.0% | |
| TCCGATTAAAAGTGAGTTGT+AGG | - | chr1.2:59905382-59905401 | MS.gene40834:intron | 35.0% | |
| TCGAAAAGTTTCTTCAAGAG+AGG | - | chr1.2:59905091-59905110 | MS.gene40834:CDS | 35.0% | |
| TGAATTGAAGATGGTAGGTT+TGG | + | chr1.2:59905564-59905583 | None:intergenic | 35.0% | |
| TGCAATTGTTCGTATTCAGA+TGG | - | chr1.2:59905972-59905991 | MS.gene40834:intron | 35.0% | |
| TTAACAACTCGTATTGTTCC+AGG | - | chr1.2:59906100-59906119 | MS.gene40834:CDS | 35.0% | |
| TTCAACATTGCTGAGAATGA+AGG | - | chr1.2:59906228-59906247 | MS.gene40834:CDS | 35.0% | |
| ! | ATTTTACAATCTCGTTTGGG+TGG | - | chr1.2:59905661-59905680 | MS.gene40834:intron | 35.0% |
| !! | CCCTACAACTCACTTTTAAT+CGG | + | chr1.2:59905386-59905405 | None:intergenic | 35.0% |
| !!! | AACAAAAGCCTTTTGAAGCA+GGG | + | chr1.2:59905949-59905968 | None:intergenic | 35.0% |
| !!! | AGTAATCTTGCTTTTTTCCC+TGG | + | chr1.2:59905166-59905185 | None:intergenic | 35.0% |
| !!! | CAATGTGTGTGTTTGGTTTT+AGG | + | chr1.2:59905641-59905660 | None:intergenic | 35.0% |
| ATCAGTCTGTCTTCAACATC+TGG | - | chr1.2:59906025-59906044 | MS.gene40834:intron | 40.0% | |
| CTGATAGCAACTTCTCCAAA+CGG | - | chr1.2:59905193-59905212 | MS.gene40834:intron | 40.0% | |
| CTTCAAGAGAGGATTAAGGT+TGG | - | chr1.2:59905102-59905121 | MS.gene40834:CDS | 40.0% | |
| GGCTAAGAGTGAATTGAAGA+TGG | + | chr1.2:59905573-59905592 | None:intergenic | 40.0% | |
| GGTCAAATACTTGAGATACC+TGG | + | chr1.2:59906121-59906140 | None:intergenic | 40.0% | |
| GGTTCAAAGGGAAAGAAGAA+GGG | - | chr1.2:59905003-59905022 | MS.gene40834:CDS | 40.0% | |
| GTTTGGGTGGTATTCAAAAC+TGG | - | chr1.2:59905674-59905693 | MS.gene40834:intron | 40.0% | |
| TGATGCTGTTACTGTTTCCA+GGG | - | chr1.2:59905146-59905165 | MS.gene40834:intron | 40.0% | |
| TGGTTCAAAGGGAAAGAAGA+AGG | - | chr1.2:59905002-59905021 | MS.gene40834:CDS | 40.0% | |
| ! | TAGGGAAAGTGACTTGTGTT+TGG | - | chr1.2:59905401-59905420 | MS.gene40834:intron | 40.0% |
| !! | ATTAAGGTTGGTGGTAAACC+TGG | - | chr1.2:59905114-59905133 | MS.gene40834:intron | 40.0% |
| !!! | ACAAAAGCCTTTTGAAGCAG+GGG | + | chr1.2:59905948-59905967 | None:intergenic | 40.0% |
| !!! | CAACAAAAGCCTTTTGAAGC+AGG | + | chr1.2:59905950-59905969 | None:intergenic | 40.0% |
| CAAGAGAGGATTAAGGTTGG+TGG | - | chr1.2:59905105-59905124 | MS.gene40834:CDS | 45.0% | |
| CATTGCTGAGAATGAAGGAG+AGG | - | chr1.2:59906233-59906252 | MS.gene40834:CDS | 45.0% | |
| GTCCATGATCTTGTCTTCCA+CGG | + | chr1.2:59905061-59905080 | None:intergenic | 45.0% | |
| GTGATGCTGTTACTGTTTCC+AGG | - | chr1.2:59905145-59905164 | MS.gene40834:intron | 45.0% | |
| TCCATGATCTTGTCTTCCAC+GGG | + | chr1.2:59905060-59905079 | None:intergenic | 45.0% | |
| ! | AGTAGATGCAGCTGGTTCAA+AGG | - | chr1.2:59904990-59905009 | MS.gene40834:CDS | 45.0% |
| ! | GTAGATGCAGCTGGTTCAAA+GGG | - | chr1.2:59904991-59905010 | MS.gene40834:CDS | 45.0% |
| ACCCGTGGAAGACAAGATCA+TGG | - | chr1.2:59905056-59905075 | MS.gene40834:CDS | 50.0% | |
| GATCTTGTCTTCCACGGGTT+TGG | + | chr1.2:59905055-59905074 | None:intergenic | 50.0% | |
| GGGTGGTATTCAAAACTGGC+TGG | - | chr1.2:59905678-59905697 | MS.gene40834:intron | 50.0% | |
| ATGGGTCGAGTAGATGCAGC+TGG | - | chr1.2:59904982-59905001 | MS.gene40834:CDS | 55.0% | |
| CACGGGTTTGGCACAGTCAA+TGG | + | chr1.2:59905043-59905062 | None:intergenic | 55.0% | |
| GGTGGTAAACCTGGTGCTCT+TGG | - | chr1.2:59905123-59905142 | MS.gene40834:intron | 55.0% | |
| ! | CATTGACTGTGCCAAACCCG+TGG | - | chr1.2:59905041-59905060 | MS.gene40834:CDS | 55.0% |
| !! | ACAGCATCACCAAGAGCACC+AGG | + | chr1.2:59905135-59905154 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 59904982 | 59906266 | 59904982 | ID=MS.gene40834 |
| chr1.2 | mRNA | 59904982 | 59906266 | 59904982 | ID=MS.gene40834.t1;Parent=MS.gene40834 |
| chr1.2 | exon | 59906034 | 59906266 | 59906034 | ID=MS.gene40834.t1.exon1;Parent=MS.gene40834.t1 |
| chr1.2 | CDS | 59906034 | 59906266 | 59906034 | ID=cds.MS.gene40834.t1;Parent=MS.gene40834.t1 |
| chr1.2 | exon | 59904982 | 59905126 | 59904982 | ID=MS.gene40834.t1.exon2;Parent=MS.gene40834.t1 |
| chr1.2 | CDS | 59904982 | 59905126 | 59904982 | ID=cds.MS.gene40834.t1;Parent=MS.gene40834.t1 |
| Gene Sequence |
| Protein sequence |
