Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055017.t1 | GAU30288.1 | 53.8 | 169 | 36 | 3 | 1 | 127 | 235 | 403 | 1.90E-29 | 138.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055017.t1 | A0A2Z6MEQ0 | 53.8 | 169 | 36 | 3 | 1 | 127 | 235 | 403 | 1.4e-29 | 138.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055017 | MS.gene063846 | 0.821074 | 4.90E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene066752 | 0.815427 | 9.30E-52 | -1.69E-46 |
| MS.gene055017 | MS.gene068453 | 0.817922 | 2.56E-52 | -1.69E-46 |
| MS.gene055017 | MS.gene069271 | 0.804123 | 2.51E-49 | -1.69E-46 |
| MS.gene055017 | MS.gene069275 | 0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene055017 | MS.gene071330 | 0.810639 | 1.04E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene20657 | 0.800409 | 1.46E-48 | -1.69E-46 |
| MS.gene055017 | MS.gene24211 | 0.82584 | 3.76E-54 | -1.69E-46 |
| MS.gene055017 | MS.gene26056 | 0.812784 | 3.56E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene28203 | 0.83506 | 2.09E-56 | -1.69E-46 |
| MS.gene055017 | MS.gene30073 | 0.830098 | 3.56E-55 | -1.69E-46 |
| MS.gene055017 | MS.gene30139 | 0.817703 | 2.87E-52 | -1.69E-46 |
| MS.gene055017 | MS.gene30580 | 0.817926 | 2.56E-52 | -1.69E-46 |
| MS.gene055017 | MS.gene34615 | 0.81404 | 1.89E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene38444 | 0.816475 | 5.43E-52 | -1.69E-46 |
| MS.gene055017 | MS.gene38850 | 0.820025 | 8.53E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene41508 | 0.807156 | 5.80E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene41509 | 0.805248 | 1.46E-49 | -1.69E-46 |
| MS.gene055017 | MS.gene42887 | 0.823937 | 1.06E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene42891 | 0.822903 | 1.85E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene50755 | 0.83621 | 1.07E-56 | -1.69E-46 |
| MS.gene055017 | MS.gene51252 | 0.829628 | 4.63E-55 | -1.69E-46 |
| MS.gene055017 | MS.gene54921 | 0.821628 | 3.65E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene56618 | 0.803907 | 2.79E-49 | -1.69E-46 |
| MS.gene055017 | MS.gene59780 | 0.830451 | 2.92E-55 | -1.69E-46 |
| MS.gene055017 | MS.gene61742 | 0.850371 | 1.77E-60 | -1.69E-46 |
| MS.gene055017 | MS.gene62748 | 0.830132 | 3.49E-55 | -1.69E-46 |
| MS.gene055017 | MS.gene63141 | 0.815255 | 1.02E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene63142 | 0.820996 | 5.10E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene65003 | 0.823934 | 1.06E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene69102 | 0.824936 | 6.16E-54 | -1.69E-46 |
| MS.gene055017 | MS.gene73540 | 0.806326 | 8.68E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene79824 | 0.81024 | 1.27E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene82668 | 0.826956 | 2.04E-54 | -1.69E-46 |
| MS.gene055017 | MS.gene82682 | 0.815486 | 9.02E-52 | -1.69E-46 |
| MS.gene055017 | MS.gene82962 | 0.832891 | 7.30E-56 | -1.69E-46 |
| MS.gene055017 | MS.gene83166 | 0.800601 | 1.34E-48 | -1.69E-46 |
| MS.gene055017 | MS.gene84001 | 0.808247 | 3.40E-50 | -1.69E-46 |
| MS.gene055017 | MS.gene88533 | 0.81305 | 3.11E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene88539 | 0.812401 | 4.32E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene88548 | 0.834602 | 2.73E-56 | -1.69E-46 |
| MS.gene055017 | MS.gene91451 | 0.823442 | 1.38E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene98550 | 0.868637 | 5.63E-66 | -1.69E-46 |
| MS.gene055017 | MS.gene98673 | 0.821982 | 3.02E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055017.t1 | MTR_7g066680 | 47.778 | 180 | 29 | 2 | 2 | 116 | 155 | 334 | 9.12e-38 | 130 |
| MS.gene055017.t1 | MTR_2g029400 | 52.740 | 146 | 31 | 3 | 2 | 110 | 261 | 405 | 2.41e-34 | 123 |
| MS.gene055017.t1 | MTR_7g058710 | 48.750 | 160 | 45 | 2 | 5 | 127 | 325 | 484 | 3.54e-33 | 120 |
| MS.gene055017.t1 | MTR_7g066260 | 54.135 | 133 | 39 | 4 | 2 | 120 | 338 | 462 | 1.11e-28 | 108 |
| MS.gene055017.t1 | MTR_7g066580 | 42.500 | 160 | 49 | 4 | 1 | 117 | 283 | 442 | 3.36e-26 | 101 |
| MS.gene055017.t1 | MTR_7g066280 | 47.287 | 129 | 51 | 3 | 2 | 120 | 269 | 390 | 1.60e-24 | 96.7 |
| MS.gene055017.t1 | MTR_7g066310 | 47.287 | 129 | 51 | 3 | 2 | 120 | 262 | 383 | 2.46e-24 | 96.3 |
| MS.gene055017.t1 | MTR_7g066340 | 48.062 | 129 | 50 | 3 | 2 | 120 | 332 | 453 | 1.47e-23 | 94.4 |
| MS.gene055017.t1 | MTR_7g058700 | 44.526 | 137 | 38 | 2 | 1 | 127 | 820 | 928 | 9.19e-23 | 92.4 |
| MS.gene055017.t1 | MTR_7g066570 | 41.104 | 163 | 52 | 4 | 1 | 123 | 325 | 483 | 1.33e-22 | 92.0 |
| MS.gene055017.t1 | MTR_7g012680 | 45.205 | 146 | 57 | 3 | 1 | 127 | 311 | 452 | 9.34e-21 | 86.7 |
| MS.gene055017.t1 | MTR_1g014060 | 43.056 | 144 | 65 | 3 | 1 | 127 | 248 | 391 | 1.01e-20 | 86.3 |
| MS.gene055017.t1 | MTR_7g066430 | 37.805 | 164 | 57 | 4 | 2 | 122 | 326 | 487 | 2.81e-20 | 85.5 |
| MS.gene055017.t1 | MTR_7g066360 | 46.032 | 126 | 53 | 2 | 5 | 120 | 329 | 449 | 3.64e-20 | 85.1 |
| MS.gene055017.t1 | MTR_7g066400 | 42.748 | 131 | 53 | 4 | 2 | 116 | 309 | 433 | 4.11e-19 | 82.0 |
| MS.gene055017.t1 | MTR_7g066290 | 41.085 | 129 | 35 | 2 | 2 | 120 | 336 | 433 | 2.33e-17 | 77.0 |
| MS.gene055017.t1 | MTR_7g066390 | 47.778 | 90 | 32 | 2 | 41 | 120 | 38 | 122 | 3.01e-16 | 70.5 |
| MS.gene055017.t1 | MTR_7g062280 | 37.975 | 158 | 66 | 5 | 2 | 127 | 335 | 492 | 1.83e-15 | 71.6 |
| MS.gene055017.t1 | MTR_7g063840 | 32.716 | 162 | 70 | 4 | 1 | 127 | 317 | 474 | 1.22e-14 | 69.3 |
| MS.gene055017.t1 | MTR_7g063720 | 34.247 | 146 | 74 | 3 | 1 | 127 | 91 | 233 | 1.29e-14 | 67.8 |
| MS.gene055017.t1 | MTR_1g041315 | 39.474 | 114 | 51 | 3 | 1 | 104 | 346 | 451 | 3.49e-14 | 68.2 |
| MS.gene055017.t1 | MTR_7g066350 | 46.825 | 126 | 46 | 4 | 2 | 116 | 330 | 445 | 3.53e-14 | 67.8 |
| MS.gene055017.t1 | MTR_7g066410 | 37.037 | 162 | 49 | 6 | 7 | 121 | 358 | 513 | 4.99e-14 | 67.4 |
| MS.gene055017.t1 | MTR_7g063920 | 33.766 | 154 | 74 | 5 | 1 | 127 | 285 | 437 | 6.54e-13 | 64.3 |
| MS.gene055017.t1 | MTR_7g063850 | 47.222 | 72 | 28 | 1 | 65 | 126 | 217 | 288 | 1.74e-11 | 60.1 |
| MS.gene055017.t1 | MTR_7g063660 | 41.111 | 90 | 43 | 1 | 48 | 127 | 389 | 478 | 6.46e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGATCTGTTGATTTACA+TGG | 0.374990 | 7.4:-37279635 | MS.gene055017:CDS |
| AATGTGTGTAACTTTAAATT+TGG | 0.383037 | 7.4:-37279707 | MS.gene055017:CDS |
| TCAACATCTGCTTTGACAAA+TGG | 0.406997 | 7.4:-37279391 | MS.gene055017:CDS |
| ATAGACAGGTAAGTTAGCTT+TGG | 0.452689 | 7.4:+37279821 | None:intergenic |
| TTACATATGTTGCAGGGTCT+CGG | 0.483160 | 7.4:-37279436 | MS.gene055017:intron |
| GGTAAGTTAGCTTTGGCGAT+GGG | 0.500925 | 7.4:+37279828 | None:intergenic |
| AAATGTTCCTATGTTTGATG+TGG | 0.550491 | 7.4:-37279548 | MS.gene055017:CDS |
| GAGCTCAAATTGTGCTGCAA+TGG | 0.555169 | 7.4:+37279866 | None:intergenic |
| TCAAATTGTGCTGCAATGGT+CGG | 0.562601 | 7.4:+37279870 | None:intergenic |
| CTTCATAACAATCATTAATG+AGG | 0.573011 | 7.4:-37279359 | MS.gene055017:CDS |
| ATTACCTCCACATCAAACAT+AGG | 0.574855 | 7.4:+37279541 | None:intergenic |
| AGGTAAGTTAGCTTTGGCGA+TGG | 0.576022 | 7.4:+37279827 | None:intergenic |
| TAGATGTGAAATAGCAGTGA+AGG | 0.576058 | 7.4:+37279579 | None:intergenic |
| TGTTCCTATGTTTGATGTGG+AGG | 0.589861 | 7.4:-37279545 | MS.gene055017:intron |
| TTACATGGTAACAGATGACT+TGG | 0.593082 | 7.4:-37279620 | MS.gene055017:CDS |
| GCTTCAACATCATCAAGTTG+TGG | 0.609223 | 7.4:-37279667 | MS.gene055017:CDS |
| GATGTTGAAGCATAGTACTT+CGG | 0.614337 | 7.4:+37279679 | None:intergenic |
| ATTTGTCAAAGCAGATGTTG+AGG | 0.617384 | 7.4:+37279393 | None:intergenic |
| ATAGCAGTGAAGGATGACAT+TGG | 0.632785 | 7.4:+37279589 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTGTGTAACTTTAAATT+TGG | - | chr7.4:37279547-37279566 | MS.gene055017:CDS | 20.0% |
| ! | ACTAGGAAAAACAATTTGAA+TGG | + | chr7.4:37279520-37279539 | None:intergenic | 25.0% |
| ! | CTAGGAAAAACAATTTGAAT+GGG | + | chr7.4:37279519-37279538 | None:intergenic | 25.0% |
| ! | CTTCATAACAATCATTAATG+AGG | - | chr7.4:37279895-37279914 | MS.gene055017:CDS | 25.0% |
| !! | CGTTATTGATTGCAATTAAT+TGG | - | chr7.4:37279762-37279781 | MS.gene055017:intron | 25.0% |
| AAATGTTCCTATGTTTGATG+TGG | - | chr7.4:37279706-37279725 | MS.gene055017:CDS | 30.0% | |
| CAAGTATGACAATAATAGAC+AGG | + | chr7.4:37279450-37279469 | None:intergenic | 30.0% | |
| CTATTATTGTCATACTTGCT+TGG | - | chr7.4:37279452-37279471 | MS.gene055017:intron | 30.0% | |
| CTTTGATTACATATGTTGCA+GGG | - | chr7.4:37279812-37279831 | MS.gene055017:intron | 30.0% | |
| TCTTTGATTACATATGTTGC+AGG | - | chr7.4:37279811-37279830 | MS.gene055017:intron | 30.0% | |
| ! | GAACTCATTGTGATAATGTA+TGG | + | chr7.4:37279793-37279812 | None:intergenic | 30.0% |
| !! | CATAGATCTGTTGATTTACA+TGG | - | chr7.4:37279619-37279638 | MS.gene055017:CDS | 30.0% |
| !! | TCAAATTGTTTTTCCTAGTC+TGG | - | chr7.4:37279521-37279540 | MS.gene055017:intron | 30.0% |
| ATTACCTCCACATCAAACAT+AGG | + | chr7.4:37279716-37279735 | None:intergenic | 35.0% | |
| ATTTGTCAAAGCAGATGTTG+AGG | + | chr7.4:37279864-37279883 | None:intergenic | 35.0% | |
| GATGTTGAAGCATAGTACTT+CGG | + | chr7.4:37279578-37279597 | None:intergenic | 35.0% | |
| TAGATGTGAAATAGCAGTGA+AGG | + | chr7.4:37279678-37279697 | None:intergenic | 35.0% | |
| TCAACATCTGCTTTGACAAA+TGG | - | chr7.4:37279863-37279882 | MS.gene055017:CDS | 35.0% | |
| TTACATGGTAACAGATGACT+TGG | - | chr7.4:37279634-37279653 | MS.gene055017:CDS | 35.0% | |
| ! | ATAGACAGGTAAGTTAGCTT+TGG | + | chr7.4:37279436-37279455 | None:intergenic | 35.0% |
| AATGGGTGTAGTACTAAGAG+TGG | + | chr7.4:37279502-37279521 | None:intergenic | 40.0% | |
| ATAGCAGTGAAGGATGACAT+TGG | + | chr7.4:37279668-37279687 | None:intergenic | 40.0% | |
| ATGGGTGTAGTACTAAGAGT+GGG | + | chr7.4:37279501-37279520 | None:intergenic | 40.0% | |
| TACACACATTTCACCAGACT+AGG | + | chr7.4:37279537-37279556 | None:intergenic | 40.0% | |
| TCAAATTGTGCTGCAATGGT+CGG | + | chr7.4:37279387-37279406 | None:intergenic | 40.0% | |
| TGTTCCTATGTTTGATGTGG+AGG | - | chr7.4:37279709-37279728 | MS.gene055017:CDS | 40.0% | |
| TTACATATGTTGCAGGGTCT+CGG | - | chr7.4:37279818-37279837 | MS.gene055017:intron | 40.0% | |
| ! | GCTTCAACATCATCAAGTTG+TGG | - | chr7.4:37279587-37279606 | MS.gene055017:CDS | 40.0% |
| GAGCTCAAATTGTGCTGCAA+TGG | + | chr7.4:37279391-37279410 | None:intergenic | 45.0% | |
| !! | AGGTAAGTTAGCTTTGGCGA+TGG | + | chr7.4:37279430-37279449 | None:intergenic | 45.0% |
| !! | GGTAAGTTAGCTTTGGCGAT+GGG | + | chr7.4:37279429-37279448 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 37279351 | 37279925 | 37279351 | ID=MS.gene055017 |
| chr7.4 | mRNA | 37279351 | 37279925 | 37279351 | ID=MS.gene055017.t1;Parent=MS.gene055017 |
| chr7.4 | exon | 37279827 | 37279925 | 37279827 | ID=MS.gene055017.t1.exon1;Parent=MS.gene055017.t1 |
| chr7.4 | CDS | 37279827 | 37279925 | 37279827 | ID=cds.MS.gene055017.t1;Parent=MS.gene055017.t1 |
| chr7.4 | exon | 37279546 | 37279737 | 37279546 | ID=MS.gene055017.t1.exon2;Parent=MS.gene055017.t1 |
| chr7.4 | CDS | 37279546 | 37279737 | 37279546 | ID=cds.MS.gene055017.t1;Parent=MS.gene055017.t1 |
| chr7.4 | exon | 37279351 | 37279443 | 37279351 | ID=MS.gene055017.t1.exon3;Parent=MS.gene055017.t1 |
| chr7.4 | CDS | 37279351 | 37279443 | 37279351 | ID=cds.MS.gene055017.t1;Parent=MS.gene055017.t1 |
| Gene Sequence |
| Protein sequence |
