AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42348


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42348.t1 MTR_3g111780 95.070 284 13 1 1 284 1 283 0.0 502
MS.gene42348.t1 MTR_8g103520 51.389 72 35 0 193 264 218 289 1.41e-20 89.4
MS.gene42348.t1 MTR_0907s0020 54.545 66 30 0 199 264 102 167 1.21e-19 84.7
MS.gene42348.t1 MTR_5g075210 43.000 100 57 0 164 263 245 344 4.79e-19 86.3
MS.gene42348.t1 MTR_7g072875 32.468 154 80 2 129 260 129 280 2.63e-18 83.2
MS.gene42348.t1 MTR_7g105790 53.623 69 32 0 202 270 113 181 5.87e-18 80.5
MS.gene42348.t1 MTR_4g052040 46.835 79 42 0 186 264 86 164 1.86e-17 78.6
MS.gene42348.t1 MTR_7g072630 26.829 287 155 8 27 260 58 342 4.57e-17 80.5
MS.gene42348.t1 MTR_5g079710 50.725 69 34 0 196 264 88 156 5.15e-17 79.0
MS.gene42348.t1 MTR_8g087530 47.692 65 34 0 199 263 258 322 6.80e-16 77.0
MS.gene42348.t1 MTR_2g018030 46.774 62 33 0 199 260 277 338 9.90e-16 76.6
MS.gene42348.t1 MTR_4g028930 50.820 61 30 0 200 260 315 375 6.48e-15 74.3
MS.gene42348.t1 MTR_4g052060 51.667 60 29 0 205 264 99 158 1.96e-13 67.8
MS.gene42348.t1 MTR_1g111640 45.161 62 34 0 198 259 136 197 3.70e-11 62.4
MS.gene42348.t1 MTR_4g132740 37.500 88 44 1 178 265 71 147 5.82e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42348.t1 AT5G19650 62.121 66 25 0 199 264 154 219 7.56e-23 94.4
MS.gene42348.t1 AT2G18500 61.538 65 25 0 199 263 226 290 2.81e-20 89.0
MS.gene42348.t1 AT2G30400 50.000 70 35 0 194 263 247 316 1.73e-18 84.3
MS.gene42348.t1 AT3G52525 56.452 62 27 0 200 261 67 128 7.01e-18 79.3
MS.gene42348.t1 AT5G58360 53.731 67 31 0 197 263 222 288 2.31e-17 80.9
MS.gene42348.t1 AT2G36026 55.000 60 27 0 205 264 92 151 5.31e-17 77.4
MS.gene42348.t1 AT1G06920 33.010 103 64 1 167 264 206 308 4.42e-16 77.4
MS.gene42348.t1 AT5G01840 48.529 68 35 0 203 270 202 269 4.05e-15 73.9
MS.gene42348.t1 AT5G22240 44.118 68 38 0 196 263 93 160 1.22e-13 68.6
MS.gene42348.t1 AT4G18830 45.902 61 33 0 199 259 282 342 8.22e-12 65.1

Find 67 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCAGGGCCTGGATTCC+CGG 0.272464 3.2:+89241673 None:intergenic
CTCAAATTATAGTGATAAAA+AGG 0.274257 3.2:-89241369 MS.gene42348:CDS
GGTCCATTCTTCTAGTACTA+TGG 0.311039 3.2:-89241414 MS.gene42348:CDS
GACACATTGCATTGGTGTTC+CGG 0.326318 3.2:+89241584 None:intergenic
TGCGAGCTTTCATGGAGATT+TGG 0.332540 3.2:-89240992 MS.gene42348:CDS
AGTAATTGTGCGAGCTTTCA+TGG 0.344864 3.2:-89241000 MS.gene42348:CDS
CACCGGAGAAAGACGGTATA+TGG 0.359937 3.2:+89241655 None:intergenic
TCCTCTTTCCCCGGGAATCC+AGG 0.367021 3.2:-89241683 MS.gene42348:CDS
TTTGGCAACAAATGTTCGTT+TGG 0.381425 3.2:-89240974 MS.gene42348:CDS
GGCGACGAAGCAGGAAGGTT+TGG 0.382600 3.2:+89241605 None:intergenic
TTATCACTATAATTTGAGAT+AGG 0.385663 3.2:+89241374 None:intergenic
ATACCCTATGTCATATGCTT+TGG 0.387292 3.2:+89241628 None:intergenic
GGTAAAAGAAAGCTTTGCTT+TGG 0.392617 3.2:-89241162 MS.gene42348:CDS
AGTGAGGTTTCAAAGTATTA+CGG 0.405418 3.2:-89241285 MS.gene42348:CDS
ATTTAACTAGCTCGAATAGA+AGG 0.424510 3.2:-89241337 MS.gene42348:CDS
AATGACCTTTCCTCTTTCCC+CGG 0.427656 3.2:-89241692 MS.gene42348:CDS
ACTTGACATAACCTTCGTTT+CGG 0.428668 3.2:+89241199 None:intergenic
GATGTCCGAGGCCAAGGACT+TGG 0.437700 3.2:-89241072 MS.gene42348:CDS
ATGGCAGGGCCTGGATTCCC+GGG 0.439698 3.2:+89241674 None:intergenic
GTTAGAGATGATAGAAGAGA+TGG 0.441612 3.2:-89241096 MS.gene42348:CDS
TTCTTGCTTAGCAAGATTCT+CGG 0.449560 3.2:-89241447 MS.gene42348:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.2:-89241192 MS.gene42348:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.2:-89241240 MS.gene42348:CDS
CTATGGATAGTACTAAAGTT+AGG 0.470027 3.2:-89241397 MS.gene42348:CDS
TGAAGAAGGTTGAGAGAGTG+AGG 0.472397 3.2:-89241301 MS.gene42348:CDS
CAATAGTTGCTCCAAGTCCT+TGG 0.478606 3.2:+89241061 None:intergenic
TCAAACAAATGTTCAAACTA+AGG 0.481359 3.2:-89240928 MS.gene42348:CDS
GGGGAAAGAGGAAAGGTCAT+TGG 0.484366 3.2:+89241694 None:intergenic
AGACGGTATATGGCAGGGCC+TGG 0.484707 3.2:+89241665 None:intergenic
ACTAGAAGAATGGACCTTCT+CGG 0.488884 3.2:+89241421 None:intergenic
GCCTGGATTCCCGGGGAAAG+AGG 0.494617 3.2:+89241682 None:intergenic
TATCCATAGTACTAGAAGAA+TGG 0.495158 3.2:+89241411 None:intergenic
AGAAACTCACTTCTTCTTGA+GGG 0.501729 3.2:+89241509 None:intergenic
TGGTGTTCCGGCGACGAAGC+AGG 0.502249 3.2:+89241596 None:intergenic
GACGATAAGTCGTGTGAAGA+AGG 0.503045 3.2:-89241315 MS.gene42348:CDS
GATGGAGATGTCCGAGGCCA+AGG 0.503048 3.2:-89241078 MS.gene42348:CDS
TGCCATATACCGTCTTTCTC+CGG 0.503136 3.2:-89241657 MS.gene42348:CDS
GAGCGACACATTTGAAAAGA+AGG 0.503682 3.2:+89241716 None:intergenic
GTCAAGTAGAATGGAGGAGA+AGG 0.514162 3.2:-89241183 MS.gene42348:CDS
AAACCTTCCTGCTTCGTCGC+CGG 0.514759 3.2:-89241603 MS.gene42348:CDS
TTGCTCCAAGTCCTTGGCCT+CGG 0.515968 3.2:+89241067 None:intergenic
AGCGACACATTTGAAAAGAA+GGG 0.522344 3.2:+89241717 None:intergenic
ATGACCTTTCCTCTTTCCCC+GGG 0.522963 3.2:-89241691 MS.gene42348:CDS
GGAGAAAGACGGTATATGGC+AGG 0.531009 3.2:+89241659 None:intergenic
TAGAAACTCACTTCTTCTTG+AGG 0.531127 3.2:+89241508 None:intergenic
TCATATGCTTTGGAGTTCAC+CGG 0.541099 3.2:+89241638 None:intergenic
CTAAATTCTAGAGATTATCA+TGG 0.544872 3.2:-89241023 MS.gene42348:CDS
GATTTCTTGAAGTCTTCGTA+AGG 0.562850 3.2:+89241122 None:intergenic
AATTGATAAAATCAATGAAG+AGG 0.573253 3.2:-89241486 MS.gene42348:CDS
AAGATTCTCGGAAGCCGAGA+AGG 0.578226 3.2:-89241435 MS.gene42348:CDS
AACTCCAAAGCATATGACAT+AGG 0.582440 3.2:-89241632 MS.gene42348:CDS
TCTTCTTGAGGGAACGTCGG+AGG 0.601171 3.2:+89241520 None:intergenic
ACTCCAAAGCATATGACATA+GGG 0.601832 3.2:-89241631 MS.gene42348:CDS
TCGAAGAGGAACCGAAACGA+AGG 0.602284 3.2:-89241210 MS.gene42348:CDS
ATTAAGAAAGTGAAATCCAA+AGG 0.608408 3.2:-89241554 MS.gene42348:CDS
TGGAGTTCACCGGAGAAAGA+CGG 0.608559 3.2:+89241648 None:intergenic
TCTTTGATGACACATTGCAT+TGG 0.617252 3.2:+89241576 None:intergenic
GTTCCGGCGACGAAGCAGGA+AGG 0.630275 3.2:+89241600 None:intergenic
GATTCCCGGGGAAAGAGGAA+AGG 0.637825 3.2:+89241687 None:intergenic
GAGAAAGACGGTATATGGCA+GGG 0.639308 3.2:+89241660 None:intergenic
ACTTCTTCTTGAGGGAACGT+CGG 0.649804 3.2:+89241517 None:intergenic
AGTATTACGGAGAATCAAAG+AGG 0.650942 3.2:-89241272 MS.gene42348:CDS
TGGCAGGGCCTGGATTCCCG+GGG 0.653477 3.2:+89241675 None:intergenic
GGTTATGTCAAGTAGAATGG+AGG 0.656874 3.2:-89241189 MS.gene42348:CDS
AGAATGGAAGAGAATCGAAG+AGG 0.657497 3.2:-89241224 MS.gene42348:CDS
TCAAAGAGGAAAAGAAACGA+AGG 0.672215 3.2:-89241258 MS.gene42348:CDS
AGAAGAGATGGAGATGTCCG+AGG 0.675634 3.2:-89241084 MS.gene42348:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATAAAATCAATGAAG+AGG - chr3.2:89241177-89241196 MS.gene42348:CDS 20.0%
!! CTCAAATTATAGTGATAAAA+AGG - chr3.2:89241294-89241313 MS.gene42348:CDS 20.0%
!!! TTATCACTATAATTTGAGAT+AGG + chr3.2:89241292-89241311 None:intergenic 20.0%
! ATTAAGAAAGTGAAATCCAA+AGG - chr3.2:89241109-89241128 MS.gene42348:CDS 25.0%
! TCAAACAAATGTTCAAACTA+AGG - chr3.2:89241735-89241754 MS.gene42348:CDS 25.0%
!! CTAAATTCTAGAGATTATCA+TGG - chr3.2:89241640-89241659 MS.gene42348:CDS 25.0%
!!! TGAAGATTTTTTACTGACTT+AGG + chr3.2:89241718-89241737 None:intergenic 25.0%
ATTTAACTAGCTCGAATAGA+AGG - chr3.2:89241326-89241345 MS.gene42348:CDS 30.0%
CTATGGATAGTACTAAAGTT+AGG - chr3.2:89241266-89241285 MS.gene42348:CDS 30.0%
TATCCATAGTACTAGAAGAA+TGG + chr3.2:89241255-89241274 None:intergenic 30.0%
! AGTGAGGTTTCAAAGTATTA+CGG - chr3.2:89241378-89241397 MS.gene42348:CDS 30.0%
AACTCCAAAGCATATGACAT+AGG - chr3.2:89241031-89241050 MS.gene42348:CDS 35.0%
ACTCCAAAGCATATGACATA+GGG - chr3.2:89241032-89241051 MS.gene42348:CDS 35.0%
ACTTGACATAACCTTCGTTT+CGG + chr3.2:89241419-89241438 None:intergenic 35.0%
AGAAACTCACTTCTTCTTGA+GGG + chr3.2:89241157-89241176 None:intergenic 35.0%
AGCGACACATTTGAAAAGAA+GGG + chr3.2:89240949-89240968 None:intergenic 35.0%
AGTATTACGGAGAATCAAAG+AGG - chr3.2:89241391-89241410 MS.gene42348:CDS 35.0%
ATACCCTATGTCATATGCTT+TGG + chr3.2:89241038-89241057 None:intergenic 35.0%
GAAGGTTATGTCAAGTAGAA+TGG - chr3.2:89241423-89241442 MS.gene42348:CDS 35.0%
GATTTCTTGAAGTCTTCGTA+AGG + chr3.2:89241544-89241563 None:intergenic 35.0%
GGTAAAAGAAAGCTTTGCTT+TGG - chr3.2:89241501-89241520 MS.gene42348:CDS 35.0%
GTTAGAGATGATAGAAGAGA+TGG - chr3.2:89241567-89241586 MS.gene42348:CDS 35.0%
TAGAAACTCACTTCTTCTTG+AGG + chr3.2:89241158-89241177 None:intergenic 35.0%
TCAAAGAGGAAAAGAAACGA+AGG - chr3.2:89241405-89241424 MS.gene42348:CDS 35.0%
TGCTTTGGTGAAAAAGTCAA+AGG - chr3.2:89241516-89241535 MS.gene42348:CDS 35.0%
TTCTTGCTTAGCAAGATTCT+CGG - chr3.2:89241216-89241235 MS.gene42348:CDS 35.0%
TTTGGCAACAAATGTTCGTT+TGG - chr3.2:89241689-89241708 MS.gene42348:CDS 35.0%
! TCTTTGATGACACATTGCAT+TGG + chr3.2:89241090-89241109 None:intergenic 35.0%
!!! GCAACTATTGCAGTGTTTTT+TGG - chr3.2:89241615-89241634 MS.gene42348:CDS 35.0%
ACTAGAAGAATGGACCTTCT+CGG + chr3.2:89241245-89241264 None:intergenic 40.0%
AGAATGGAAGAGAATCGAAG+AGG - chr3.2:89241439-89241458 MS.gene42348:CDS 40.0%
AGTAATTGTGCGAGCTTTCA+TGG - chr3.2:89241663-89241682 MS.gene42348:CDS 40.0%
GAGCGACACATTTGAAAAGA+AGG + chr3.2:89240950-89240969 None:intergenic 40.0%
GGTCCATTCTTCTAGTACTA+TGG - chr3.2:89241249-89241268 MS.gene42348:CDS 40.0%
GGTTATGTCAAGTAGAATGG+AGG - chr3.2:89241426-89241445 MS.gene42348:CDS 40.0%
!! TCATATGCTTTGGAGTTCAC+CGG + chr3.2:89241028-89241047 None:intergenic 40.0%
AATGACCTTTCCTCTTTCCC+CGG - chr3.2:89240971-89240990 MS.gene42348:CDS 45.0%
ACTTCTTCTTGAGGGAACGT+CGG + chr3.2:89241149-89241168 None:intergenic 45.0%
CAATAGTTGCTCCAAGTCCT+TGG + chr3.2:89241605-89241624 None:intergenic 45.0%
GACGATAAGTCGTGTGAAGA+AGG - chr3.2:89241348-89241367 MS.gene42348:CDS 45.0%
GAGAAAGACGGTATATGGCA+GGG + chr3.2:89241006-89241025 None:intergenic 45.0%
GTCAAGTAGAATGGAGGAGA+AGG - chr3.2:89241480-89241499 MS.gene42348:CDS 45.0%
TGAAGAAGGTTGAGAGAGTG+AGG - chr3.2:89241362-89241381 MS.gene42348:CDS 45.0%
TGCCATATACCGTCTTTCTC+CGG - chr3.2:89241006-89241025 MS.gene42348:CDS 45.0%
TGCGAGCTTTCATGGAGATT+TGG - chr3.2:89241671-89241690 MS.gene42348:CDS 45.0%
! GACACATTGCATTGGTGTTC+CGG + chr3.2:89241082-89241101 None:intergenic 45.0%
AAGATTCTCGGAAGCCGAGA+AGG - chr3.2:89241228-89241247 MS.gene42348:CDS 50.0%
AGAAGAGATGGAGATGTCCG+AGG - chr3.2:89241579-89241598 MS.gene42348:CDS 50.0%
ATGACCTTTCCTCTTTCCCC+GGG - chr3.2:89240972-89240991 MS.gene42348:CDS 50.0%
CACCGGAGAAAGACGGTATA+TGG + chr3.2:89241011-89241030 None:intergenic 50.0%
GGAGAAAGACGGTATATGGC+AGG + chr3.2:89241007-89241026 None:intergenic 50.0%
GGGGAAAGAGGAAAGGTCAT+TGG + chr3.2:89240972-89240991 None:intergenic 50.0%
TCGAAGAGGAACCGAAACGA+AGG - chr3.2:89241453-89241472 MS.gene42348:CDS 50.0%
TGGAGTTCACCGGAGAAAGA+CGG + chr3.2:89241018-89241037 None:intergenic 50.0%
AAACCTTCCTGCTTCGTCGC+CGG - chr3.2:89241060-89241079 MS.gene42348:CDS 55.0%
GATTCCCGGGGAAAGAGGAA+AGG + chr3.2:89240979-89240998 None:intergenic 55.0%
TCTTCTTGAGGGAACGTCGG+AGG + chr3.2:89241146-89241165 None:intergenic 55.0%
TTGCTCCAAGTCCTTGGCCT+CGG + chr3.2:89241599-89241618 None:intergenic 55.0%
!!! GAACGTCGGAGGTGCTTTTG+CGG + chr3.2:89241135-89241154 None:intergenic 55.0%
AGACGGTATATGGCAGGGCC+TGG + chr3.2:89241001-89241020 None:intergenic 60.0%
GATGGAGATGTCCGAGGCCA+AGG - chr3.2:89241585-89241604 MS.gene42348:CDS 60.0%
GATGTCCGAGGCCAAGGACT+TGG - chr3.2:89241591-89241610 MS.gene42348:CDS 60.0%
GGCGACGAAGCAGGAAGGTT+TGG + chr3.2:89241061-89241080 None:intergenic 60.0%
TATGGCAGGGCCTGGATTCC+CGG + chr3.2:89240993-89241012 None:intergenic 60.0%
TCCTCTTTCCCCGGGAATCC+AGG - chr3.2:89240980-89240999 MS.gene42348:CDS 60.0%
!!! GGAGGTGCTTTTGCGGCCTT+TGG + chr3.2:89241128-89241147 None:intergenic 60.0%
ATGGCAGGGCCTGGATTCCC+GGG + chr3.2:89240992-89241011 None:intergenic 65.0%
GCCTGGATTCCCGGGGAAAG+AGG + chr3.2:89240984-89241003 None:intergenic 65.0%
GTTCCGGCGACGAAGCAGGA+AGG + chr3.2:89241066-89241085 None:intergenic 65.0%
TGGTGTTCCGGCGACGAAGC+AGG + chr3.2:89241070-89241089 None:intergenic 65.0%
TGGCAGGGCCTGGATTCCCG+GGG + chr3.2:89240991-89241010 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 89240914 89241771 89240914 ID=MS.gene42348
chr3.2 mRNA 89240914 89241771 89240914 ID=MS.gene42348.t1;Parent=MS.gene42348
chr3.2 exon 89240914 89241771 89240914 ID=MS.gene42348.t1.exon1;Parent=MS.gene42348.t1
chr3.2 CDS 89240914 89241771 89240914 ID=cds.MS.gene42348.t1;Parent=MS.gene42348.t1
Gene Sequence

>MS.gene42348

ATGTCTAAACGCTTTAAGATGAAATTTACAATCCCTTCTTTTCAAATGTGTCGCTCCAATGACCTTTCCTCTTTCCCCGGGAATCCAGGCCCTGCCATATACCGTCTTTCTCCGGTGAACTCCAAAGCATATGACATAGGGTATCCAAACCTTCCTGCTTCGTCGCCGGAACACCAATGCAATGTGTCATCAAAGATTAAGAAAGTGAAATCCAAAGGCCGCAAAAGCACCTCCGACGTTCCCTCAAGAAGAAGTGAGTTTCTAATTGATAAAATCAATGAAGAGGAAAGTGAATCATTGATTTCTTGCTTAGCAAGATTCTCGGAAGCCGAGAAGGTCCATTCTTCTAGTACTATGGATAGTACTAAAGTTAGGCCTATCTCAAATTATAGTGATAAAAAGGAGAATAAAGATTTAACTAGCTCGAATAGAAGGACGATAAGTCGTGTGAAGAAGGTTGAGAGAGTGAGGTTTCAAAGTATTACGGAGAATCAAAGAGGAAAAGAAACGAAGGTTATGTCAAGTAGAATGGAAGAGAATCGAAGAGGAACCGAAACGAAGGTTATGTCAAGTAGAATGGAGGAGAAGGTAAAAGAAAGCTTTGCTTTGGTGAAAAAGTCAAAGGATCCTTACGAAGACTTCAAGAAATCAATGTTAGAGATGATAGAAGAGATGGAGATGTCCGAGGCCAAGGACTTGGAGCAACTATTGCAGTGTTTTTTGGCTCTAAATTCTAGAGATTATCATGGAGTAATTGTGCGAGCTTTCATGGAGATTTGGCAACAAATGTTCGTTTGGAATCCTAAGTCAGTAAAAAATCTTCAAACAAATGTTCAAACTAAGGATGTAGAAAATTAG

Protein sequence

>MS.gene42348.t1

MSKRFKMKFTIPSFQMCRSNDLSSFPGNPGPAIYRLSPVNSKAYDIGYPNLPASSPEHQCNVSSKIKKVKSKGRKSTSDVPSRRSEFLIDKINEEESESLISCLARFSEAEKVHSSSTMDSTKVRPISNYSDKKENKDLTSSNRRTISRVKKVERVRFQSITENQRGKETKVMSSRMEENRRGTETKVMSSRMEEKVKESFALVKKSKDPYEDFKKSMLEMIEEMEMSEAKDLEQLLQCFLALNSRDYHGVIVRAFMEIWQQMFVWNPKSVKNLQTNVQTKDVEN