Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43322.t1 | XP_022855746.1 | 64.5 | 138 | 30 | 1 | 1 | 138 | 1 | 119 | 2.00E-40 | 175.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43322.t1 | Q852N6 | 77.9 | 95 | 21 | 0 | 1 | 95 | 459 | 553 | 1.2e-38 | 160.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43322.t1 | A0A4V6ACL0 | 63.8 | 138 | 26 | 1 | 1 | 138 | 462 | 575 | 7.2e-40 | 172.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene43322 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene04917 | MS.gene43322 | 0.803557 | 3.29E-49 | -1.69E-46 |
| MS.gene04984 | MS.gene43322 | 0.800189 | 1.62E-48 | -1.69E-46 |
| MS.gene050114 | MS.gene43322 | 0.802655 | 5.06E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene43322 | 0.820695 | 5.99E-53 | -1.69E-46 |
| MS.gene055864 | MS.gene43322 | 0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene058758 | MS.gene43322 | 0.801408 | 9.13E-49 | -1.69E-46 |
| MS.gene059050 | MS.gene43322 | 0.800699 | 1.27E-48 | -1.69E-46 |
| MS.gene059170 | MS.gene43322 | 0.80524 | 1.47E-49 | -1.69E-46 |
| MS.gene060870 | MS.gene43322 | 0.809376 | 1.95E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene43322 | 0.811181 | 7.96E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43322.t1 | MTR_1g041150 | 81.884 | 138 | 5 | 1 | 1 | 138 | 464 | 581 | 4.69e-71 | 223 |
| MS.gene43322.t1 | MTR_1g055255 | 63.971 | 136 | 30 | 2 | 1 | 136 | 470 | 586 | 1.48e-52 | 174 |
| MS.gene43322.t1 | MTR_5g099240 | 68.750 | 112 | 35 | 0 | 1 | 112 | 370 | 481 | 3.37e-47 | 159 |
| MS.gene43322.t1 | MTR_5g099240 | 68.750 | 112 | 35 | 0 | 1 | 112 | 442 | 553 | 9.37e-47 | 159 |
| MS.gene43322.t1 | MTR_7g106710 | 73.684 | 95 | 25 | 0 | 1 | 95 | 481 | 575 | 1.15e-45 | 156 |
| MS.gene43322.t1 | MTR_1g054865 | 73.913 | 92 | 24 | 0 | 1 | 92 | 507 | 598 | 1.29e-43 | 151 |
| MS.gene43322.t1 | MTR_3g098090 | 67.327 | 101 | 33 | 0 | 1 | 101 | 378 | 478 | 5.74e-43 | 147 |
| MS.gene43322.t1 | MTR_1g096490 | 62.069 | 116 | 37 | 1 | 1 | 116 | 376 | 484 | 9.02e-43 | 147 |
| MS.gene43322.t1 | MTR_3g098070 | 66.337 | 101 | 34 | 0 | 1 | 101 | 375 | 475 | 4.47e-42 | 145 |
| MS.gene43322.t1 | MTR_8g095440 | 63.551 | 107 | 39 | 0 | 1 | 107 | 376 | 482 | 4.78e-42 | 145 |
| MS.gene43322.t1 | MTR_7g106710 | 74.359 | 78 | 20 | 0 | 1 | 78 | 481 | 558 | 1.17e-35 | 129 |
| MS.gene43322.t1 | MTR_5g009830 | 61.458 | 96 | 36 | 1 | 1 | 95 | 412 | 507 | 1.47e-34 | 125 |
| MS.gene43322.t1 | MTR_0028s0170 | 60.417 | 96 | 37 | 1 | 1 | 95 | 424 | 519 | 6.05e-34 | 124 |
| MS.gene43322.t1 | MTR_8g099095 | 59.375 | 96 | 38 | 1 | 1 | 95 | 412 | 507 | 2.83e-33 | 122 |
| MS.gene43322.t1 | MTR_5g089320 | 58.333 | 96 | 39 | 1 | 1 | 95 | 414 | 509 | 9.10e-33 | 120 |
| MS.gene43322.t1 | MTR_1g026190 | 58.763 | 97 | 39 | 1 | 1 | 96 | 428 | 524 | 3.62e-32 | 119 |
| MS.gene43322.t1 | MTR_5g092810 | 56.842 | 95 | 40 | 1 | 1 | 94 | 438 | 532 | 2.67e-31 | 116 |
| MS.gene43322.t1 | MTR_4g132070 | 53.398 | 103 | 46 | 2 | 1 | 102 | 437 | 538 | 6.61e-31 | 115 |
| MS.gene43322.t1 | MTR_3g051770 | 52.427 | 103 | 41 | 1 | 1 | 95 | 429 | 531 | 8.24e-30 | 112 |
| MS.gene43322.t1 | MTR_5g089440 | 55.914 | 93 | 39 | 2 | 5 | 95 | 88 | 180 | 9.80e-30 | 106 |
| MS.gene43322.t1 | MTR_4g132040 | 49.505 | 101 | 49 | 2 | 1 | 100 | 441 | 540 | 4.05e-27 | 105 |
| MS.gene43322.t1 | MTR_4g107490 | 50.000 | 96 | 46 | 2 | 1 | 94 | 419 | 514 | 7.09e-25 | 99.0 |
| MS.gene43322.t1 | MTR_7g068710 | 47.423 | 97 | 49 | 1 | 1 | 95 | 402 | 498 | 1.91e-23 | 94.7 |
| MS.gene43322.t1 | MTR_1g052530 | 47.000 | 100 | 45 | 3 | 1 | 95 | 398 | 494 | 6.51e-21 | 87.8 |
| MS.gene43322.t1 | MTR_7g091890 | 43.000 | 100 | 49 | 3 | 1 | 95 | 404 | 500 | 4.77e-19 | 82.4 |
| MS.gene43322.t1 | MTR_4g066660 | 38.947 | 95 | 57 | 1 | 1 | 94 | 418 | 512 | 8.26e-17 | 75.9 |
| MS.gene43322.t1 | MTR_1g076650 | 38.462 | 91 | 53 | 2 | 4 | 91 | 56 | 146 | 1.05e-11 | 58.9 |
| MS.gene43322.t1 | MTR_5g022030 | 38.235 | 102 | 53 | 3 | 1 | 96 | 448 | 545 | 1.27e-11 | 61.2 |
| MS.gene43322.t1 | MTR_0168s0120 | 37.363 | 91 | 54 | 2 | 4 | 91 | 20 | 110 | 1.93e-11 | 57.4 |
| MS.gene43322.t1 | MTR_6g025320 | 37.363 | 91 | 54 | 2 | 4 | 91 | 20 | 110 | 1.93e-11 | 57.4 |
| MS.gene43322.t1 | MTR_7g087610 | 37.363 | 91 | 54 | 2 | 4 | 91 | 56 | 146 | 3.92e-11 | 57.4 |
| MS.gene43322.t1 | MTR_5g088320 | 37.363 | 91 | 54 | 2 | 4 | 91 | 56 | 146 | 5.21e-11 | 57.0 |
| MS.gene43322.t1 | MTR_6g025320 | 37.363 | 91 | 54 | 2 | 4 | 91 | 56 | 146 | 5.79e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43322.t1 | AT5G04870 | 64.662 | 133 | 28 | 2 | 1 | 133 | 496 | 609 | 5.20e-52 | 174 |
| MS.gene43322.t1 | AT3G10660 | 63.158 | 133 | 30 | 2 | 1 | 133 | 532 | 645 | 7.26e-48 | 163 |
| MS.gene43322.t1 | AT3G10660 | 63.158 | 133 | 30 | 2 | 1 | 133 | 532 | 645 | 7.26e-48 | 163 |
| MS.gene43322.t1 | AT2G17290 | 66.964 | 112 | 37 | 0 | 1 | 112 | 431 | 542 | 3.35e-45 | 154 |
| MS.gene43322.t1 | AT2G17290 | 66.964 | 112 | 37 | 0 | 1 | 112 | 431 | 542 | 3.35e-45 | 154 |
| MS.gene43322.t1 | AT2G38910 | 73.913 | 92 | 24 | 0 | 1 | 92 | 480 | 571 | 1.87e-44 | 153 |
| MS.gene43322.t1 | AT4G38230 | 72.340 | 94 | 26 | 0 | 1 | 94 | 226 | 319 | 6.73e-44 | 147 |
| MS.gene43322.t1 | AT4G35310 | 66.071 | 112 | 38 | 0 | 1 | 112 | 443 | 554 | 1.81e-43 | 150 |
| MS.gene43322.t1 | AT4G35310 | 66.071 | 112 | 38 | 0 | 1 | 112 | 443 | 554 | 1.81e-43 | 150 |
| MS.gene43322.t1 | AT4G38230 | 72.340 | 94 | 26 | 0 | 1 | 94 | 370 | 463 | 7.32e-43 | 147 |
| MS.gene43322.t1 | AT4G38230 | 72.340 | 94 | 26 | 0 | 1 | 94 | 370 | 463 | 7.32e-43 | 147 |
| MS.gene43322.t1 | AT4G38230 | 72.340 | 94 | 26 | 0 | 1 | 94 | 400 | 493 | 9.11e-43 | 147 |
| MS.gene43322.t1 | AT4G09570 | 69.474 | 95 | 29 | 0 | 1 | 95 | 371 | 465 | 2.00e-41 | 144 |
| MS.gene43322.t1 | AT1G35670 | 69.474 | 95 | 29 | 0 | 1 | 95 | 372 | 466 | 3.17e-41 | 143 |
| MS.gene43322.t1 | AT5G23580 | 69.892 | 93 | 28 | 0 | 1 | 93 | 368 | 460 | 1.11e-39 | 139 |
| MS.gene43322.t1 | AT5G12180 | 64.583 | 96 | 33 | 1 | 1 | 95 | 419 | 514 | 8.25e-38 | 134 |
| MS.gene43322.t1 | AT5G19360 | 64.583 | 96 | 33 | 1 | 1 | 95 | 414 | 509 | 1.32e-37 | 134 |
| MS.gene43322.t1 | AT4G21940 | 53.846 | 104 | 46 | 2 | 1 | 102 | 447 | 550 | 1.75e-31 | 117 |
| MS.gene43322.t1 | AT4G21940 | 53.846 | 104 | 46 | 2 | 1 | 102 | 447 | 550 | 1.81e-31 | 117 |
| MS.gene43322.t1 | AT1G76040 | 53.125 | 96 | 44 | 1 | 1 | 95 | 219 | 314 | 3.70e-31 | 113 |
| MS.gene43322.t1 | AT1G76040 | 53.125 | 96 | 44 | 1 | 1 | 95 | 219 | 314 | 3.70e-31 | 113 |
| MS.gene43322.t1 | AT4G23650 | 56.250 | 96 | 41 | 1 | 1 | 95 | 424 | 519 | 5.57e-31 | 115 |
| MS.gene43322.t1 | AT4G04710 | 52.427 | 103 | 48 | 1 | 1 | 102 | 364 | 466 | 2.07e-30 | 114 |
| MS.gene43322.t1 | AT4G04710 | 52.427 | 103 | 48 | 1 | 1 | 102 | 378 | 480 | 2.39e-30 | 114 |
| MS.gene43322.t1 | AT4G04710 | 52.427 | 103 | 48 | 1 | 1 | 102 | 391 | 493 | 2.63e-30 | 114 |
| MS.gene43322.t1 | AT1G76040 | 53.125 | 96 | 44 | 1 | 1 | 95 | 457 | 552 | 5.59e-30 | 113 |
| MS.gene43322.t1 | AT4G04720 | 49.515 | 103 | 51 | 1 | 1 | 102 | 425 | 527 | 9.40e-30 | 112 |
| MS.gene43322.t1 | AT4G04720 | 49.515 | 103 | 51 | 1 | 1 | 102 | 425 | 527 | 9.40e-30 | 112 |
| MS.gene43322.t1 | AT4G04710 | 51.485 | 101 | 48 | 1 | 3 | 102 | 395 | 495 | 6.95e-29 | 110 |
| MS.gene43322.t1 | AT1G50700 | 50.485 | 103 | 49 | 2 | 1 | 102 | 419 | 520 | 1.00e-28 | 109 |
| MS.gene43322.t1 | AT3G20410 | 51.042 | 96 | 46 | 1 | 1 | 95 | 437 | 532 | 1.05e-27 | 107 |
| MS.gene43322.t1 | AT3G20410 | 51.042 | 96 | 46 | 1 | 1 | 95 | 437 | 532 | 1.05e-27 | 107 |
| MS.gene43322.t1 | AT4G04695 | 51.042 | 96 | 46 | 1 | 1 | 95 | 377 | 472 | 1.79e-27 | 105 |
| MS.gene43322.t1 | AT1G50700 | 50.000 | 100 | 48 | 2 | 4 | 102 | 448 | 546 | 1.97e-27 | 106 |
| MS.gene43322.t1 | AT1G61950 | 51.613 | 93 | 44 | 1 | 1 | 92 | 445 | 537 | 6.65e-26 | 102 |
| MS.gene43322.t1 | AT4G04740 | 44.118 | 102 | 56 | 1 | 1 | 101 | 414 | 515 | 9.37e-25 | 98.6 |
| MS.gene43322.t1 | AT4G04740 | 44.118 | 102 | 56 | 1 | 1 | 101 | 414 | 515 | 9.37e-25 | 98.6 |
| MS.gene43322.t1 | AT4G04740 | 44.118 | 102 | 56 | 1 | 1 | 101 | 414 | 515 | 9.37e-25 | 98.6 |
| MS.gene43322.t1 | AT4G04740 | 44.118 | 102 | 56 | 1 | 1 | 101 | 414 | 515 | 9.37e-25 | 98.6 |
| MS.gene43322.t1 | AT4G04740 | 44.118 | 102 | 56 | 1 | 1 | 101 | 414 | 515 | 9.37e-25 | 98.6 |
| MS.gene43322.t1 | AT4G04740 | 44.118 | 102 | 56 | 1 | 1 | 101 | 414 | 515 | 9.37e-25 | 98.6 |
| MS.gene43322.t1 | AT1G74740 | 48.454 | 97 | 48 | 2 | 1 | 95 | 405 | 501 | 1.16e-24 | 98.2 |
| MS.gene43322.t1 | AT3G51850 | 49.485 | 97 | 47 | 1 | 1 | 95 | 400 | 496 | 2.39e-24 | 97.4 |
| MS.gene43322.t1 | AT1G18890 | 49.485 | 97 | 47 | 2 | 1 | 95 | 409 | 505 | 4.73e-24 | 96.7 |
| MS.gene43322.t1 | AT4G04700 | 48.387 | 93 | 47 | 1 | 1 | 92 | 377 | 469 | 1.25e-22 | 92.4 |
| MS.gene43322.t1 | AT5G19450 | 47.000 | 100 | 45 | 3 | 1 | 95 | 404 | 500 | 4.64e-22 | 90.9 |
| MS.gene43322.t1 | AT5G19450 | 47.000 | 100 | 45 | 3 | 1 | 95 | 404 | 500 | 4.64e-22 | 90.9 |
| MS.gene43322.t1 | AT3G57530 | 44.792 | 96 | 52 | 1 | 1 | 95 | 409 | 504 | 2.12e-21 | 89.0 |
| MS.gene43322.t1 | AT5G12480 | 43.000 | 100 | 50 | 3 | 1 | 95 | 311 | 408 | 7.23e-20 | 84.7 |
| MS.gene43322.t1 | AT5G12480 | 43.000 | 100 | 50 | 3 | 1 | 95 | 405 | 502 | 9.97e-20 | 84.3 |
| MS.gene43322.t1 | AT4G04740 | 46.250 | 80 | 42 | 1 | 1 | 79 | 414 | 493 | 4.07e-19 | 82.8 |
| MS.gene43322.t1 | AT2G41860 | 42.268 | 97 | 54 | 1 | 1 | 95 | 400 | 496 | 4.34e-18 | 79.7 |
| MS.gene43322.t1 | AT2G41860 | 42.268 | 97 | 54 | 1 | 1 | 95 | 400 | 496 | 4.78e-18 | 79.7 |
| MS.gene43322.t1 | AT3G51850 | 48.101 | 79 | 39 | 1 | 1 | 77 | 400 | 478 | 4.02e-17 | 77.0 |
| MS.gene43322.t1 | AT2G17890 | 38.235 | 102 | 53 | 3 | 1 | 96 | 457 | 554 | 4.94e-15 | 70.9 |
| MS.gene43322.t1 | AT2G31500 | 37.000 | 100 | 58 | 2 | 1 | 95 | 412 | 511 | 5.79e-15 | 70.9 |
| MS.gene43322.t1 | AT1G76040 | 60.417 | 48 | 19 | 0 | 1 | 48 | 457 | 504 | 1.47e-14 | 69.7 |
| MS.gene43322.t1 | AT4G36070 | 38.235 | 102 | 53 | 3 | 1 | 96 | 420 | 517 | 6.21e-14 | 67.8 |
| MS.gene43322.t1 | AT4G36070 | 38.235 | 102 | 53 | 3 | 1 | 96 | 420 | 517 | 6.92e-14 | 67.8 |
| MS.gene43322.t1 | AT4G21940 | 63.043 | 46 | 17 | 0 | 1 | 46 | 447 | 492 | 8.34e-14 | 67.4 |
| MS.gene43322.t1 | AT3G59440 | 34.000 | 100 | 62 | 1 | 1 | 96 | 92 | 191 | 2.14e-12 | 61.6 |
| MS.gene43322.t1 | AT4G14640 | 40.000 | 90 | 51 | 2 | 5 | 91 | 40 | 129 | 5.09e-12 | 59.7 |
| MS.gene43322.t1 | AT1G66410 | 38.462 | 91 | 53 | 2 | 4 | 91 | 20 | 110 | 6.18e-12 | 58.9 |
| MS.gene43322.t1 | AT1G66410 | 38.462 | 91 | 53 | 2 | 4 | 91 | 20 | 110 | 6.18e-12 | 58.9 |
| MS.gene43322.t1 | AT5G37780 | 38.462 | 91 | 53 | 2 | 4 | 91 | 43 | 133 | 7.52e-12 | 59.3 |
| MS.gene43322.t1 | AT4G14640 | 40.000 | 90 | 51 | 2 | 5 | 91 | 58 | 147 | 1.21e-11 | 58.9 |
| MS.gene43322.t1 | AT1G50700 | 56.522 | 46 | 20 | 0 | 1 | 46 | 419 | 464 | 1.22e-11 | 61.2 |
| MS.gene43322.t1 | AT5G37780 | 38.462 | 91 | 53 | 2 | 4 | 91 | 56 | 146 | 1.70e-11 | 58.5 |
| MS.gene43322.t1 | AT1G66410 | 38.462 | 91 | 53 | 2 | 4 | 91 | 56 | 146 | 1.70e-11 | 58.5 |
| MS.gene43322.t1 | AT1G66410 | 38.462 | 91 | 53 | 2 | 4 | 91 | 66 | 156 | 1.70e-11 | 58.5 |
| MS.gene43322.t1 | AT2G27030 | 35.185 | 108 | 64 | 3 | 4 | 105 | 56 | 163 | 1.78e-11 | 58.9 |
| MS.gene43322.t1 | AT5G37780 | 38.462 | 91 | 53 | 2 | 4 | 91 | 71 | 161 | 1.98e-11 | 58.5 |
| MS.gene43322.t1 | AT3G43810 | 37.363 | 91 | 54 | 2 | 4 | 91 | 20 | 110 | 2.02e-11 | 57.4 |
| MS.gene43322.t1 | AT3G43810 | 37.363 | 91 | 54 | 2 | 4 | 91 | 20 | 110 | 2.02e-11 | 57.4 |
| MS.gene43322.t1 | AT2G41110 | 38.462 | 91 | 53 | 2 | 4 | 91 | 68 | 158 | 2.31e-11 | 58.5 |
| MS.gene43322.t1 | AT3G56800 | 38.462 | 91 | 53 | 2 | 4 | 91 | 56 | 146 | 2.75e-11 | 57.8 |
| MS.gene43322.t1 | AT2G27030 | 38.462 | 91 | 53 | 2 | 4 | 91 | 56 | 146 | 2.75e-11 | 57.8 |
| MS.gene43322.t1 | AT2G41110 | 38.462 | 91 | 53 | 2 | 4 | 91 | 56 | 146 | 2.75e-11 | 57.8 |
| MS.gene43322.t1 | AT5G37780 | 38.462 | 91 | 53 | 2 | 4 | 91 | 82 | 172 | 2.85e-11 | 58.5 |
| MS.gene43322.t1 | AT3G43810 | 37.363 | 91 | 54 | 2 | 4 | 91 | 56 | 146 | 6.06e-11 | 57.0 |
| MS.gene43322.t1 | AT5G21274 | 37.363 | 91 | 54 | 2 | 4 | 91 | 56 | 146 | 6.19e-11 | 57.0 |
Find 18 sgRNAs with CRISPR-Local
Find 156 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGTGGGACAATTGATTA+TGG | 0.379412 | 1.4:+33775983 | MS.gene43322:CDS |
| CAACAAGCTAGCGACGAGTT+TGG | 0.398048 | 1.4:+33776121 | MS.gene43322:CDS |
| TAACTTCAGCATTCGTTTCA+AGG | 0.399330 | 1.4:+33777821 | MS.gene43322:CDS |
| CTTCCTATGGTTGGTAAGAA+AGG | 0.415345 | 1.4:+33777789 | MS.gene43322:CDS |
| ATCTTTATCAAAGTAAGAAA+AGG | 0.472805 | 1.4:-33776068 | None:intergenic |
| TGGCTTTAAAGATGTTCGTT+TGG | 0.475471 | 1.4:+33776141 | MS.gene43322:CDS |
| TACTTTGATAAAGATGGAAG+TGG | 0.530734 | 1.4:+33776076 | MS.gene43322:CDS |
| AAAGGAAATCTTCCTATGGT+TGG | 0.545060 | 1.4:+33777780 | MS.gene43322:CDS |
| GCAGAAAGGAAATCTTCCTA+TGG | 0.570793 | 1.4:+33777672 | MS.gene43322:CDS |
| GCAGAAAGGAAATCTTCCTA+TGG | 0.570793 | 1.4:+33777776 | MS.gene43322:CDS |
| TTTGCATCTATGATGCAGAA+AGG | 0.580419 | 1.4:+33777762 | MS.gene43322:CDS |
| AATTTATGATTGAATGCAAG+CGG | 0.582834 | 1.4:+33775526 | None:intergenic |
| AGACCTTTCTTACCAACCAT+AGG | 0.593466 | 1.4:-33777792 | None:intergenic |
| TTTGCATCTATGATGCAGAA+AGG | 0.600020 | 1.4:+33777658 | MS.gene43322:CDS |
| AGGCTGATGTAGATAACAGT+GGG | 0.651767 | 1.4:+33775969 | MS.gene43322:intron |
| TGATTATGGTGAGTTCATTG+CGG | 0.658251 | 1.4:+33775997 | MS.gene43322:CDS |
| TAGGCTGATGTAGATAACAG+TGG | 0.668045 | 1.4:+33775968 | MS.gene43322:intron |
| CTTCAGCATTCGTTTCAAGG+AGG | 0.668858 | 1.4:+33777824 | MS.gene43322:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATTCATATTACTTAAT+AGG | - | chr1.4:33776554-33776573 | None:intergenic | 10.0% |
| !! | CTAATATTCATTCAAATATA+TGG | + | chr1.4:33777113-33777132 | MS.gene43322:intron | 15.0% |
| !! | CTCTATTTAATAAAAAAGAT+TGG | - | chr1.4:33776442-33776461 | None:intergenic | 15.0% |
| !! | TTTATAAACATGTCATAAAT+TGG | - | chr1.4:33777256-33777275 | None:intergenic | 15.0% |
| !!! | TCTTTTTTATTAAATAGAGA+AGG | + | chr1.4:33776443-33776462 | MS.gene43322:intron | 15.0% |
| !! | ACAATTCTATTGAACTATAT+AGG | - | chr1.4:33777012-33777031 | None:intergenic | 20.0% |
| !! | ATCTTTATCAAAGTAAGAAA+AGG | - | chr1.4:33776071-33776090 | None:intergenic | 20.0% |
| !! | ATGCTATAAACAATTGATTA+AGG | + | chr1.4:33777413-33777432 | MS.gene43322:intron | 20.0% |
| !! | CTCTTCAAAAAAAAAAAAAC+AGG | + | chr1.4:33775905-33775924 | MS.gene43322:intron | 20.0% |
| !! | TGCAAAAAATTAATGTATAC+TGG | - | chr1.4:33776654-33776673 | None:intergenic | 20.0% |
| !! | TGCTATAAACAATTGATTAA+GGG | + | chr1.4:33777414-33777433 | MS.gene43322:intron | 20.0% |
| !!! | ACTTAGGCTTTAAAAAAATA+TGG | + | chr1.4:33776935-33776954 | MS.gene43322:intron | 20.0% |
| !!! | ATTTTTTTAAAGCCTAAGTT+TGG | - | chr1.4:33776934-33776953 | None:intergenic | 20.0% |
| !!! | CTGTTTTTTTTTTTTTGAAG+AGG | - | chr1.4:33775907-33775926 | None:intergenic | 20.0% |
| !!! | TAAGATATAGACTTTTAAAC+AGG | + | chr1.4:33776837-33776856 | MS.gene43322:intron | 20.0% |
| !!! | TTACAAAAATAAGTTGAGAA+CGG | - | chr1.4:33777279-33777298 | None:intergenic | 20.0% |
| !!! | TTTAATAAAAAAGATTGGTC+TGG | - | chr1.4:33776437-33776456 | None:intergenic | 20.0% |
| !!! | TTTTCTTACTTTGATAAAGA+TGG | + | chr1.4:33776070-33776089 | MS.gene43322:CDS | 20.0% |
| ! | AACTACAAATATTGAAGAGA+AGG | - | chr1.4:33776230-33776249 | None:intergenic | 25.0% |
| ! | ACCATAATTATTTGAGAAAG+TGG | + | chr1.4:33776292-33776311 | MS.gene43322:intron | 25.0% |
| ! | ATATCATTACCACAACAAAT+AGG | - | chr1.4:33775874-33775893 | None:intergenic | 25.0% |
| ! | ATCTACTAACATAAACTGTT+TGG | + | chr1.4:33777216-33777235 | MS.gene43322:intron | 25.0% |
| ! | ATTAAGGGTTTATCCAAATA+AGG | + | chr1.4:33777429-33777448 | MS.gene43322:intron | 25.0% |
| ! | ATTCATATTACTTAATAGGC+CGG | - | chr1.4:33776550-33776569 | None:intergenic | 25.0% |
| ! | GTATCTGAGAAATATTTACA+AGG | - | chr1.4:33775802-33775821 | None:intergenic | 25.0% |
| ! | GTTAAAATAATTACCAAGTG+AGG | + | chr1.4:33777141-33777160 | MS.gene43322:intron | 25.0% |
| ! | GTTAATGTGTTCTATTTAAC+AGG | + | chr1.4:33775599-33775618 | MS.gene43322:intron | 25.0% |
| ! | TAATGAAGAACTTAGATGTA+AGG | - | chr1.4:33777064-33777083 | None:intergenic | 25.0% |
| ! | TCATTATTAGAAATTCATCG+TGG | + | chr1.4:33777078-33777097 | MS.gene43322:intron | 25.0% |
| ! | TCCACTTTCTCAAATAATTA+TGG | - | chr1.4:33776296-33776315 | None:intergenic | 25.0% |
| ! | TCTACTAACATAAACTGTTT+GGG | + | chr1.4:33777217-33777236 | MS.gene43322:intron | 25.0% |
| ! | TTAAGGGTTTATCCAAATAA+GGG | + | chr1.4:33777430-33777449 | MS.gene43322:intron | 25.0% |
| ! | TTGTTCCTTTCTACTTTATA+AGG | - | chr1.4:33775572-33775591 | None:intergenic | 25.0% |
| !! | CTTTCTTTCTTATTGTCTTT+TGG | + | chr1.4:33775763-33775782 | MS.gene43322:intron | 25.0% |
| !! | TCTTTTACTGTTCATTACAA+TGG | - | chr1.4:33777535-33777554 | None:intergenic | 25.0% |
| !! | TTAAGCCTATTTAGCTTAAA+AGG | + | chr1.4:33776482-33776501 | MS.gene43322:intron | 25.0% |
| AAAAGCCTCTTAAGCATATT+AGG | + | chr1.4:33776524-33776543 | MS.gene43322:intron | 30.0% | |
| AACACTTCAATTTGAAAGTG+TGG | - | chr1.4:33776388-33776407 | None:intergenic | 30.0% | |
| AAGCGGTAAGTATCTTATAA+AGG | + | chr1.4:33775543-33775562 | MS.gene43322:intron | 30.0% | |
| ACTATATTCTTACCAACCAT+AGG | - | chr1.4:33777691-33777710 | None:intergenic | 30.0% | |
| ATCGATGAAGATAATGTAAG+TGG | + | chr1.4:33776181-33776200 | MS.gene43322:intron | 30.0% | |
| CAATGTGATCCAAGTTTAAA+TGG | - | chr1.4:33776799-33776818 | None:intergenic | 30.0% | |
| CTACTAACATAAACTGTTTG+GGG | + | chr1.4:33777218-33777237 | MS.gene43322:intron | 30.0% | |
| TACCACTTACTTTATCTGAT+AGG | - | chr1.4:33777503-33777522 | None:intergenic | 30.0% | |
| TACTAACATAAACTGTTTGG+GGG | + | chr1.4:33777219-33777238 | MS.gene43322:intron | 30.0% | |
| TACTTTGATAAAGATGGAAG+TGG | + | chr1.4:33776076-33776095 | MS.gene43322:CDS | 30.0% | |
| TCACTATACCTTGAAATTGT+AGG | + | chr1.4:33775949-33775968 | MS.gene43322:intron | 30.0% | |
| ! | ATAATCAACACGAGGTTTTA+GGG | - | chr1.4:33776735-33776754 | None:intergenic | 30.0% |
| ! | CACTTTCAAATTGAAGTGTT+AGG | + | chr1.4:33776388-33776407 | MS.gene43322:intron | 30.0% |
| ! | TAGTGGTTATAAGTGCTTTT+GGG | - | chr1.4:33777466-33777485 | None:intergenic | 30.0% |
| ! | TCTTTCTTATTGTCTTTTGG+TGG | + | chr1.4:33775766-33775785 | MS.gene43322:intron | 30.0% |
| !! | AGGCTTTAAAAAAATATGGC+AGG | + | chr1.4:33776939-33776958 | MS.gene43322:intron | 30.0% |
| !!! | AGCATCAGATTTTGACTATA+AGG | - | chr1.4:33777601-33777620 | None:intergenic | 30.0% |
| !!! | CTTATTTTTGTAAGCTCTCT+AGG | + | chr1.4:33777286-33777305 | MS.gene43322:intron | 30.0% |
| AAAGGAAATCTTCCTATGGT+TGG | + | chr1.4:33777676-33777695 | MS.gene43322:intron | 35.0% | |
| AACAGTGGGACAATTGATTA+TGG | + | chr1.4:33775983-33776002 | MS.gene43322:CDS | 35.0% | |
| ACTTTATCTGATAGGCGATA+CGG | - | chr1.4:33777495-33777514 | None:intergenic | 35.0% | |
| AGTCTTCCCCTTAATTATCA+TGG | + | chr1.4:33775693-33775712 | MS.gene43322:intron | 35.0% | |
| CACCAACCATGATAATTAAG+GGG | - | chr1.4:33775702-33775721 | None:intergenic | 35.0% | |
| CCAATTCCAAGAATAGAGAA+CGG | - | chr1.4:33775732-33775751 | None:intergenic | 35.0% | |
| CCACCAACCATGATAATTAA+GGG | - | chr1.4:33775703-33775722 | None:intergenic | 35.0% | |
| CTATTCTTGGAATTGGCTAA+CGG | + | chr1.4:33775736-33775755 | MS.gene43322:intron | 35.0% | |
| CTGATGCTTTATCTTATGCA+GGG | + | chr1.4:33777613-33777632 | MS.gene43322:intron | 35.0% | |
| GAACTATATAGGCCTAACTA+GGG | - | chr1.4:33777001-33777020 | None:intergenic | 35.0% | |
| GGACTCCTTATAAAGTAGAA+AGG | + | chr1.4:33775564-33775583 | MS.gene43322:intron | 35.0% | |
| TAACTTCAGCATTCGTTTCA+AGG | + | chr1.4:33777821-33777840 | MS.gene43322:CDS | 35.0% | |
| TACATCAGCCTACAATTTCA+AGG | - | chr1.4:33775960-33775979 | None:intergenic | 35.0% | |
| TCAGATACTTGTTTGTACTC+AGG | + | chr1.4:33775814-33775833 | MS.gene43322:intron | 35.0% | |
| TCTGATGCTTTATCTTATGC+AGG | + | chr1.4:33777612-33777631 | MS.gene43322:intron | 35.0% | |
| TGAACTATATAGGCCTAACT+AGG | - | chr1.4:33777002-33777021 | None:intergenic | 35.0% | |
| TGATTATGGTGAGTTCATTG+CGG | + | chr1.4:33775997-33776016 | MS.gene43322:CDS | 35.0% | |
| TGTCAGACAAAGACATTTGT+CGG | + | chr1.4:33776355-33776374 | MS.gene43322:intron | 35.0% | |
| TTCCCCTTAATTATCATGGT+TGG | + | chr1.4:33775697-33775716 | MS.gene43322:intron | 35.0% | |
| TTCGCTAGTCCATTTAAACT+TGG | + | chr1.4:33776787-33776806 | MS.gene43322:intron | 35.0% | |
| TTGAACCTAACCACATGTAT+CGG | + | chr1.4:33776321-33776340 | MS.gene43322:intron | 35.0% | |
| TTTGCATCTATGATGCAGAA+AGG | + | chr1.4:33777658-33777677 | MS.gene43322:CDS | 35.0% | |
| ! | AAATTGAAGTGTTAGGCCTA+GGG | + | chr1.4:33776395-33776414 | MS.gene43322:intron | 35.0% |
| ! | AGACTTTTAAACAGGCTTGT+AGG | + | chr1.4:33776845-33776864 | MS.gene43322:intron | 35.0% |
| ! | AGTGGTTATAAGTGCTTTTG+GGG | - | chr1.4:33777465-33777484 | None:intergenic | 35.0% |
| ! | CTTCAATTTGAAAGTGTGGT+CGG | - | chr1.4:33776384-33776403 | None:intergenic | 35.0% |
| ! | GATAATCAACACGAGGTTTT+AGG | - | chr1.4:33776736-33776755 | None:intergenic | 35.0% |
| ! | GTAGTGGTTATAAGTGCTTT+TGG | - | chr1.4:33777467-33777486 | None:intergenic | 35.0% |
| ! | TGCTTAAGAGGCTTTTTGAA+AGG | - | chr1.4:33776520-33776539 | None:intergenic | 35.0% |
| !! | TCTTATTGTCTTTTGGTGGT+TGG | + | chr1.4:33775770-33775789 | MS.gene43322:intron | 35.0% |
| !! | TGGCTTTAAAGATGTTCGTT+TGG | + | chr1.4:33776141-33776160 | MS.gene43322:CDS | 35.0% |
| AAACTGTTTGGGGGAACTTA+TGG | + | chr1.4:33777228-33777247 | MS.gene43322:intron | 40.0% | |
| AAATTCATCGTGGTTGACAG+TGG | + | chr1.4:33777088-33777107 | MS.gene43322:intron | 40.0% | |
| AGACCTTTCTTACCAACCAT+AGG | - | chr1.4:33777795-33777814 | None:intergenic | 40.0% | |
| AGGCTGATGTAGATAACAGT+GGG | + | chr1.4:33775969-33775988 | MS.gene43322:intron | 40.0% | |
| ATATAGGCCTAACTAGGGAT+GGG | - | chr1.4:33776996-33777015 | None:intergenic | 40.0% | |
| ATGCTTTATCTTATGCAGGG+CGG | + | chr1.4:33777616-33777635 | MS.gene43322:intron | 40.0% | |
| CAGTCTCAAGCCTAAAAAGT+AGG | + | chr1.4:33776890-33776909 | MS.gene43322:intron | 40.0% | |
| CCCTTAATTATCATGGTTGG+TGG | + | chr1.4:33775700-33775719 | MS.gene43322:intron | 40.0% | |
| CCGTTCTCTATTCTTGGAAT+TGG | + | chr1.4:33775729-33775748 | MS.gene43322:intron | 40.0% | |
| CGCCTATCAGATAAAGTAAG+TGG | + | chr1.4:33777498-33777517 | MS.gene43322:intron | 40.0% | |
| CTAAAAAGTAGGCCTATGAC+AGG | + | chr1.4:33776901-33776920 | MS.gene43322:intron | 40.0% | |
| CTTAGTCCGTTCTCTATTCT+TGG | + | chr1.4:33775723-33775742 | MS.gene43322:intron | 40.0% | |
| GCAGAAAGGAAATCTTCCTA+TGG | + | chr1.4:33777672-33777691 | MS.gene43322:CDS | 40.0% | |
| GCCACCAACCATGATAATTA+AGG | - | chr1.4:33775704-33775723 | None:intergenic | 40.0% | |
| GGGTTGAGTTAACCCTTATT+TGG | - | chr1.4:33777445-33777464 | None:intergenic | 40.0% | |
| TAGGCTGATGTAGATAACAG+TGG | + | chr1.4:33775968-33775987 | MS.gene43322:intron | 40.0% | |
| TATATAGGCCTAACTAGGGA+TGG | - | chr1.4:33776997-33777016 | None:intergenic | 40.0% | |
| TTAAGTCACTCACGTTCACA+TGG | + | chr1.4:33775652-33775671 | MS.gene43322:intron | 40.0% | |
| ! | CAAATTGAAGTGTTAGGCCT+AGG | + | chr1.4:33776394-33776413 | MS.gene43322:intron | 40.0% |
| ! | CCTATTTAGCTTAAAAGGCC+AGG | + | chr1.4:33776487-33776506 | MS.gene43322:intron | 40.0% |
| ! | CTCTCTTGATAATCAACACG+AGG | - | chr1.4:33776743-33776762 | None:intergenic | 40.0% |
| ! | TTAATTTTGTCCACCACCTG+CGG | - | chr1.4:33775636-33775655 | None:intergenic | 40.0% |
| !! | CCTGGCCTTTTAAGCTAAAT+AGG | - | chr1.4:33776490-33776509 | None:intergenic | 40.0% |
| !! | CTTCCTATGGTTGGTAAGAA+AGG | + | chr1.4:33777685-33777704 | MS.gene43322:intron | 40.0% |
| !! | TAAAAAAGATTGGTCTGGCC+TGG | - | chr1.4:33776432-33776451 | None:intergenic | 40.0% |
| !!! | CTACTTTTTAGGCTTGAGAC+TGG | - | chr1.4:33776892-33776911 | None:intergenic | 40.0% |
| !!! | CTGTCATAGGCCTACTTTTT+AGG | - | chr1.4:33776903-33776922 | None:intergenic | 40.0% |
| ACTCACGTTCACATGGTTGA+AGG | + | chr1.4:33775659-33775678 | MS.gene43322:intron | 45.0% | |
| AGACACAACACCGATACATG+TGG | - | chr1.4:33776334-33776353 | None:intergenic | 45.0% | |
| CAACACCGATACATGTGGTT+AGG | - | chr1.4:33776329-33776348 | None:intergenic | 45.0% | |
| CTCACGTTCACATGGTTGAA+GGG | + | chr1.4:33775660-33775679 | MS.gene43322:intron | 45.0% | |
| CTTCAGCATTCGTTTCAAGG+AGG | + | chr1.4:33777824-33777843 | MS.gene43322:CDS | 45.0% | |
| GCCTCTTAAGCATATTAGGC+CGG | + | chr1.4:33776528-33776547 | MS.gene43322:intron | 45.0% | |
| TAAAGCCTAAGTTTGGCCTG+CGG | - | chr1.4:33776927-33776946 | None:intergenic | 45.0% | |
| TCACGTTCACATGGTTGAAG+GGG | + | chr1.4:33775661-33775680 | MS.gene43322:intron | 45.0% | |
| TGCTTTATCTTATGCAGGGC+GGG | + | chr1.4:33777617-33777636 | MS.gene43322:intron | 45.0% | |
| ! | CATCAAGTGGGCTTAGAACT+AGG | + | chr1.4:33777164-33777183 | MS.gene43322:intron | 45.0% |
| ! | TACCAAGTGAGGCATCAAGT+GGG | + | chr1.4:33777152-33777171 | MS.gene43322:intron | 45.0% |
| ! | TTACCAAGTGAGGCATCAAG+TGG | + | chr1.4:33777151-33777170 | MS.gene43322:intron | 45.0% |
| !! | GGTCTTTTGAAAGCCTGATG+CGG | - | chr1.4:33776871-33776890 | None:intergenic | 45.0% |
| AAATAGGCCCTCCTAGCACA+AGG | - | chr1.4:33775858-33775877 | None:intergenic | 50.0% | |
| ACAGGCTAACTCAAATCCGC+AGG | + | chr1.4:33775617-33775636 | MS.gene43322:intron | 50.0% | |
| ACTAGGGATGGGAATAGGCT+AGG | - | chr1.4:33776985-33777004 | None:intergenic | 50.0% | |
| AGGCCTTACAAAGCCTAGCT+CGG | + | chr1.4:33776959-33776978 | MS.gene43322:intron | 50.0% | |
| AGGCGATACGGCTAAAGTAG+TGG | - | chr1.4:33777483-33777502 | None:intergenic | 50.0% | |
| CAACAAGCTAGCGACGAGTT+TGG | + | chr1.4:33776121-33776140 | MS.gene43322:CDS | 50.0% | |
| GCCGGCCTAATATGCTTAAG+AGG | - | chr1.4:33776532-33776551 | None:intergenic | 50.0% | |
| GCCTAACTAGGGATGGGAAT+AGG | - | chr1.4:33776990-33777009 | None:intergenic | 50.0% | |
| GCCTATTCCCATCCCTAGTT+AGG | + | chr1.4:33776986-33777005 | MS.gene43322:intron | 50.0% | |
| TAACTCAAATCCGCAGGTGG+TGG | + | chr1.4:33775623-33775642 | MS.gene43322:intron | 50.0% | |
| TAGCACACCTTGTGCTAGGA+GGG | + | chr1.4:33775848-33775867 | MS.gene43322:intron | 50.0% | |
| TCCTAGCACAAGGTGTGCTA+AGG | - | chr1.4:33775848-33775867 | None:intergenic | 50.0% | |
| TCCTTAGCACACCTTGTGCT+AGG | + | chr1.4:33775844-33775863 | MS.gene43322:intron | 50.0% | |
| TTAGCACACCTTGTGCTAGG+AGG | + | chr1.4:33775847-33775866 | MS.gene43322:intron | 50.0% | |
| TTAGGCCTAGGGATGGGAAT+AGG | + | chr1.4:33776406-33776425 | MS.gene43322:intron | 50.0% | |
| ! | CTAGGAGGGCCTATTTGTTG+TGG | + | chr1.4:33775862-33775881 | MS.gene43322:intron | 50.0% |
| ! | GAAGTGTTAGGCCTAGGGAT+GGG | + | chr1.4:33776400-33776419 | MS.gene43322:intron | 50.0% |
| ! | TGAAGTGTTAGGCCTAGGGA+TGG | + | chr1.4:33776399-33776418 | MS.gene43322:intron | 50.0% |
| !! | GAGGCTTTTTGAAAGGCCTG+TGG | - | chr1.4:33776513-33776532 | None:intergenic | 50.0% |
| !! | TTTTTGAAAGGCCTGTGGCC+TGG | - | chr1.4:33776508-33776527 | None:intergenic | 50.0% |
| AGCCCACTTGATGCCTCACT+TGG | - | chr1.4:33777157-33777176 | None:intergenic | 55.0% | |
| AGCTTAAAAGGCCAGGCCAC+AGG | + | chr1.4:33776494-33776513 | MS.gene43322:intron | 55.0% | |
| GGCTAACTCAAATCCGCAGG+TGG | + | chr1.4:33775620-33775639 | MS.gene43322:intron | 55.0% | |
| ! | AGGCCGAGCTAGGCTTTGTA+AGG | - | chr1.4:33776965-33776984 | None:intergenic | 55.0% |
| ACAGGCCGCAGGCCAAACTT+AGG | + | chr1.4:33776919-33776938 | MS.gene43322:intron | 60.0% | |
| ACAGGCTTGTAGGCCGCATC+AGG | + | chr1.4:33776855-33776874 | MS.gene43322:intron | 60.0% | |
| CCTAGGGATGGGAATAGGCC+AGG | + | chr1.4:33776411-33776430 | MS.gene43322:intron | 60.0% | |
| CCTGGCCTATTCCCATCCCT+AGG | - | chr1.4:33776414-33776433 | None:intergenic | 60.0% | |
| GGAATAGGCTAGGCCGAGCT+AGG | - | chr1.4:33776975-33776994 | None:intergenic | 60.0% | |
| TTTGGCCTGCGGCCTGTCAT+AGG | - | chr1.4:33776916-33776935 | None:intergenic | 60.0% | |
| GTAGGCCTATGACAGGCCGC+AGG | + | chr1.4:33776908-33776927 | MS.gene43322:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 33775539 | 33777860 | 33775539 | ID=MS.gene43322 |
| chr1.4 | mRNA | 33775539 | 33777860 | 33775539 | ID=MS.gene43322.t1;Parent=MS.gene43322 |
| chr1.4 | exon | 33775539 | 33775547 | 33775539 | ID=MS.gene43322.t1.exon1;Parent=MS.gene43322.t1 |
| chr1.4 | CDS | 33775539 | 33775547 | 33775539 | ID=cds.MS.gene43322.t1;Parent=MS.gene43322.t1 |
| chr1.4 | exon | 33775971 | 33776195 | 33775971 | ID=MS.gene43322.t1.exon2;Parent=MS.gene43322.t1 |
| chr1.4 | CDS | 33775971 | 33776195 | 33775971 | ID=cds.MS.gene43322.t1;Parent=MS.gene43322.t1 |
| chr1.4 | exon | 33777634 | 33777693 | 33777634 | ID=MS.gene43322.t1.exon3;Parent=MS.gene43322.t1 |
| chr1.4 | CDS | 33777634 | 33777693 | 33777634 | ID=cds.MS.gene43322.t1;Parent=MS.gene43322.t1 |
| chr1.4 | exon | 33777738 | 33777860 | 33777738 | ID=MS.gene43322.t1.exon4;Parent=MS.gene43322.t1 |
| chr1.4 | CDS | 33777738 | 33777860 | 33777738 | ID=cds.MS.gene43322.t1;Parent=MS.gene43322.t1 |
| Gene Sequence |
| Protein sequence |
