AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43330.t1 MTR_1g031080 83.478 345 4 1 1 345 1 292 0.0 565
MS.gene43330.t1 MTR_1g031060 81.503 346 63 1 1 345 17 362 0.0 513
MS.gene43330.t1 MTR_1g048320 74.640 347 79 5 4 345 2 344 1.92e-175 490
MS.gene43330.t1 MTR_2g087610 52.561 371 147 7 1 345 1 368 4.61e-115 338
MS.gene43330.t1 MTR_3g106930 52.189 297 113 4 64 345 95 377 1.21e-94 286
MS.gene43330.t1 MTR_1g110290 35.312 320 174 10 33 342 95 391 2.51e-48 167
MS.gene43330.t1 MTR_2g005350 37.241 290 151 7 63 342 168 436 2.86e-48 168
MS.gene43330.t1 MTR_1g009960 38.783 263 133 11 63 319 111 351 1.04e-46 162
MS.gene43330.t1 MTR_3g108740 34.848 330 166 10 34 335 63 371 1.97e-46 162
MS.gene43330.t1 MTR_1g009960 36.424 302 141 12 63 335 111 390 3.00e-46 162
MS.gene43330.t1 MTR_1g016880 34.256 289 160 7 63 342 106 373 3.04e-46 161
MS.gene43330.t1 MTR_8g015500 34.602 289 160 7 63 342 143 411 3.47e-46 162
MS.gene43330.t1 MTR_2g037600 34.237 295 149 6 61 329 103 378 5.55e-46 161
MS.gene43330.t1 MTR_4g127550 33.553 304 156 8 58 335 102 385 2.60e-45 159
MS.gene43330.t1 MTR_5g006930 33.544 316 178 9 37 342 91 384 5.51e-45 158
MS.gene43330.t1 MTR_5g055480 36.770 291 149 9 63 342 122 388 1.00e-44 157
MS.gene43330.t1 MTR_5g006920 34.494 316 175 10 37 342 126 419 2.05e-44 157
MS.gene43330.t1 MTR_2g037620 32.320 362 195 9 6 337 36 377 2.32e-44 157
MS.gene43330.t1 MTR_6g083830 33.123 317 178 9 37 342 124 417 7.80e-43 153
MS.gene43330.t1 MTR_3g104840 33.333 315 162 8 37 342 116 391 4.00e-41 148
MS.gene43330.t1 MTR_2g024120 31.902 326 154 12 63 342 61 364 1.17e-31 122
MS.gene43330.t1 MTR_8g094180 31.228 285 137 10 62 312 54 313 5.52e-30 117
MS.gene43330.t1 MTR_8g094180 29.341 334 154 12 62 338 54 362 6.07e-30 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43330.t1 AT3G52060 58.044 317 112 4 37 345 43 346 2.62e-132 381
MS.gene43330.t1 AT5G22070 52.210 362 156 8 1 345 1 362 2.95e-128 372
MS.gene43330.t1 AT5G25330 48.525 305 123 7 60 345 73 362 1.73e-86 265
MS.gene43330.t1 AT4G32290 48.837 301 121 4 64 345 93 379 1.23e-84 261
MS.gene43330.t1 AT4G31350 35.905 337 168 9 26 335 51 366 8.23e-54 181
MS.gene43330.t1 AT4G31350 35.905 337 168 9 26 335 51 366 8.23e-54 181
MS.gene43330.t1 AT4G31350 35.905 337 168 9 26 335 51 366 8.23e-54 181
MS.gene43330.t1 AT4G31350 35.905 337 163 10 26 335 51 361 5.08e-52 176
MS.gene43330.t1 AT5G25970 35.959 292 157 8 60 342 115 385 7.26e-50 171
MS.gene43330.t1 AT5G25970 35.959 292 157 8 60 342 115 385 7.26e-50 171
MS.gene43330.t1 AT5G25970 35.959 292 157 8 60 342 115 385 7.26e-50 171
MS.gene43330.t1 AT1G51770 35.294 289 157 7 63 342 123 390 6.53e-48 166
MS.gene43330.t1 AT5G11730 34.948 289 158 7 63 342 117 384 1.22e-47 165
MS.gene43330.t1 AT1G10280 36.897 290 152 9 63 342 142 410 4.12e-46 162
MS.gene43330.t1 AT3G21310 35.640 289 156 8 63 342 114 381 9.87e-46 160
MS.gene43330.t1 AT3G21310 35.640 289 156 8 63 342 114 381 9.87e-46 160
MS.gene43330.t1 AT4G30060 35.216 301 148 8 64 337 109 389 3.65e-45 159
MS.gene43330.t1 AT4G30060 35.216 301 148 8 64 337 109 389 3.65e-45 159
MS.gene43330.t1 AT2G19160 35.880 301 146 9 64 337 106 386 1.40e-44 157
MS.gene43330.t1 AT2G19160 35.880 301 146 9 64 337 106 386 1.40e-44 157
MS.gene43330.t1 AT1G10880 33.442 308 173 9 40 338 96 380 1.55e-41 149
MS.gene43330.t1 AT5G16170 33.220 295 158 8 64 342 136 407 2.73e-41 149
MS.gene43330.t1 AT4G25870 33.993 303 150 9 64 337 99 380 4.78e-41 148
MS.gene43330.t1 AT5G57270 35.000 280 150 8 62 333 96 351 7.48e-41 147
MS.gene43330.t1 AT5G57270 35.000 280 150 8 62 333 96 351 7.48e-41 147
MS.gene43330.t1 AT5G57270 35.000 280 150 8 62 333 96 351 7.48e-41 147
MS.gene43330.t1 AT5G57270 35.000 280 150 8 62 333 96 351 7.48e-41 147
MS.gene43330.t1 AT5G57270 35.000 280 150 8 62 333 96 351 7.48e-41 147
MS.gene43330.t1 AT1G68390 32.302 291 165 7 63 342 137 406 1.65e-38 142
MS.gene43330.t1 AT1G68380 33.851 322 170 11 37 343 98 391 3.16e-38 140
MS.gene43330.t1 AT1G51770 32.872 289 137 8 63 342 123 363 5.13e-36 134
MS.gene43330.t1 AT1G73810 34.915 295 151 10 64 342 147 416 1.65e-34 131
MS.gene43330.t1 AT5G14550 29.373 303 170 8 42 324 43 321 1.69e-33 127
MS.gene43330.t1 AT5G14550 27.299 348 177 12 42 337 43 366 3.13e-30 119
MS.gene43330.t1 AT1G11940 30.523 344 166 15 47 342 57 375 5.51e-30 118
MS.gene43330.t1 AT5G14550 26.994 326 180 10 42 333 43 344 6.48e-30 118
MS.gene43330.t1 AT1G62305 30.312 320 153 12 55 327 60 356 3.98e-27 110
MS.gene43330.t1 AT5G14550 26.946 334 169 13 42 337 43 339 3.59e-23 99.0
MS.gene43330.t1 AT1G62305 32.068 237 120 8 55 271 60 275 5.59e-23 97.8
MS.gene43330.t1 AT3G21310 41.322 121 69 2 63 182 114 233 2.94e-22 94.7
MS.gene43330.t1 AT1G11940 33.649 211 114 8 47 252 57 246 1.36e-21 93.2

Find 100 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGATTCCTTGTGTAAATT+TGG 0.194070 1.4:-33582109 MS.gene43330:CDS
TTACACTGACCAGAGTTAAT+TGG 0.225794 1.4:-33582014 MS.gene43330:CDS
TGGATAAGTGGGTGGTGGTG+TGG 0.269984 1.4:+33582706 None:intergenic
GTCGGAAAATAATGCTCTTC+TGG 0.270436 1.4:+33582075 None:intergenic
GTTGAAGAGATGGTTGTTTC+CGG 0.278745 1.4:+33582596 None:intergenic
AGGGGATACGACGGAGGTGG+TGG 0.323021 1.4:+33582554 None:intergenic
GGATAAGTGGGTGGTGGTGT+GGG 0.332097 1.4:+33582707 None:intergenic
GGAGTTGTTGGGAGAGGAAG+AGG 0.356101 1.4:+33582796 None:intergenic
TAAGTGGGTGGTGGTGTGGG+TGG 0.365938 1.4:+33582710 None:intergenic
ATAAGCTATGGAAGAAGTTT+CGG 0.367619 1.4:-33582128 MS.gene43330:CDS
TGGAAGAGATGAAACGGTTA+TGG 0.369541 1.4:+33582520 None:intergenic
AGGGCCAGGTCATCGGCGTC+GGG 0.373907 1.4:+33582747 None:intergenic
GTGGGTGGTGGTGTGGGTGG+TGG 0.377134 1.4:+33582713 None:intergenic
GAGAAAGGGAAGGTGAATTG+TGG 0.388358 1.4:+33582771 None:intergenic
GGGTGGTGGCACGGTGGAAG+AGG 0.389635 1.4:+33582727 None:intergenic
GGAATGTCTTGCGCCGTTGA+TGG 0.402161 1.4:-33581869 MS.gene43330:CDS
AGGTTTAGGGTTTGTGAGGG+TGG 0.408760 1.4:+33582686 None:intergenic
TGAATTGTGGGAGGAGTTGT+TGG 0.411962 1.4:+33582784 None:intergenic
GGTTTGTGAGGGTGGATAAG+TGG 0.419000 1.4:+33582694 None:intergenic
TGGAGAGAATGTTATGTTGC+CGG 0.421375 1.4:-33582232 MS.gene43330:CDS
GTTTGTGAGGGTGGATAAGT+GGG 0.436623 1.4:+33582695 None:intergenic
GAGGGCCAGGTCATCGGCGT+CGG 0.438937 1.4:+33582746 None:intergenic
GAGTTAATTGGACTGGTTGT+TGG 0.445489 1.4:-33582002 MS.gene43330:CDS
AGTTAATTGGACTGGTTGTT+GGG 0.450706 1.4:-33582001 MS.gene43330:CDS
GGGATACGACGGAGGTGGTG+GGG 0.452133 1.4:+33582556 None:intergenic
AAAGCAAAGTATTGGTTAAG+TGG 0.453008 1.4:+33582423 None:intergenic
GAGAGAATCTCTATATAGCT+AGG 0.459879 1.4:+33582291 None:intergenic
TGGTGGTGTGGGTGGTGGCA+CGG 0.462193 1.4:+33582718 None:intergenic
GTTGCCGGAGGTTCCGTTCG+AGG 0.463881 1.4:-33582217 MS.gene43330:CDS
AGAGCTAGAGAGACAAGAAT+TGG 0.468069 1.4:+33582840 None:intergenic
TGGCACGGTGGAAGAGGGCC+AGG 0.471718 1.4:+33582733 None:intergenic
GAGAAACTAAAGCAAAGTAT+TGG 0.471858 1.4:+33582415 None:intergenic
GTGGGAGGAGTTGTTGGGAG+AGG 0.472374 1.4:+33582790 None:intergenic
CGGAGGTGGTGGGGTCTGCA+TGG 0.475322 1.4:+33582565 None:intergenic
ACCACCTCCGTCGTATCCCC+TGG 0.479754 1.4:-33582552 MS.gene43330:CDS
ATCAACGGCGCAAGACATTC+CGG 0.482713 1.4:+33581871 None:intergenic
GGGAGAGGAAGAGGACGAGT+GGG 0.485804 1.4:+33582805 None:intergenic
GGGGATACGACGGAGGTGGT+GGG 0.489281 1.4:+33582555 None:intergenic
TGAAGCACGTTGGGTTGGTT+TGG 0.493655 1.4:+33582500 None:intergenic
CGGTTATGGAAGACACCGCC+AGG 0.500731 1.4:+33582534 None:intergenic
AGAAAGGGAAGGTGAATTGT+GGG 0.501893 1.4:+33582772 None:intergenic
GGTGGTGGCACGGTGGAAGA+GGG 0.504324 1.4:+33582728 None:intergenic
GTGGAAGAGGGCCAGGTCAT+CGG 0.508067 1.4:+33582740 None:intergenic
GAGTGATGGTGAAGCACGTT+GGG 0.516508 1.4:+33582491 None:intergenic
AAATCCTCGAACGGAACCTC+CGG 0.518413 1.4:+33582213 None:intergenic
TTATAACGCTCGTAGAGATT+CGG 0.519118 1.4:+33582261 None:intergenic
GGCCAGGTCATCGGCGTCGG+GGG 0.519778 1.4:+33582749 None:intergenic
TGGTGAGAGATTATAAGCTA+TGG 0.524269 1.4:-33582140 MS.gene43330:CDS
GAATTGTGGGAGGAGTTGTT+GGG 0.524774 1.4:+33582785 None:intergenic
GGTTGGTGTTTGTGAGGAAG+AGG 0.529965 1.4:+33582652 None:intergenic
TGGGAGAGGAAGAGGACGAG+TGG 0.530108 1.4:+33582804 None:intergenic
TTGTCAATGGAGGATCTGAA+TGG 0.532445 1.4:-33582048 MS.gene43330:CDS
GGACGAGTGGGAGAGAGATG+AGG 0.537328 1.4:+33582817 None:intergenic
GAGGGTGGATAAGTGGGTGG+TGG 0.541141 1.4:+33582701 None:intergenic
TACTCTAGAGGAAGTGTCGC+CGG 0.543821 1.4:-33581959 MS.gene43330:CDS
TTGGTTTGGAAGAGATGAAA+CGG 0.547600 1.4:+33582514 None:intergenic
GAGGATCTGAATGGGTGCAC+TGG 0.548273 1.4:-33582039 MS.gene43330:CDS
CTGACCAGAGTTAATTGGAC+TGG 0.559122 1.4:-33582009 MS.gene43330:CDS
TAAAGGAGCAAAAGTGAGGT+TGG 0.560959 1.4:+33582635 None:intergenic
TGTCAATGGAGGATCTGAAT+GGG 0.561186 1.4:-33582047 MS.gene43330:CDS
CACCGAGCAAGCTTCAATCC+CGG 0.562100 1.4:+33581824 None:intergenic
GGGCCAGGTCATCGGCGTCG+GGG 0.567095 1.4:+33582748 None:intergenic
AATTGGACTGGTTGTTGGGA+TGG 0.570319 1.4:-33581997 MS.gene43330:CDS
CTCAGCTGTCGAATTAGCTC+CGG 0.574566 1.4:+33581940 None:intergenic
TTTAGGTTTAGGGTTTGTGA+GGG 0.574889 1.4:+33582683 None:intergenic
AGAATTGGTGATGACAACAT+TGG 0.580475 1.4:+33582855 None:intergenic
CCCATAAAGGAGCAAAAGTG+AGG 0.582221 1.4:+33582631 None:intergenic
GACACCGCCAGGGGATACGA+CGG 0.582306 1.4:+33582545 None:intergenic
GATGGTGAAGCACGTTGGGT+TGG 0.582509 1.4:+33582495 None:intergenic
AGAGAATCTCTATATAGCTA+GGG 0.585181 1.4:+33582292 None:intergenic
GCCAGGGGATACGACGGAGG+TGG 0.585534 1.4:+33582551 None:intergenic
TCCTCCATTGACAAAAGAGT+CGG 0.587143 1.4:+33582057 None:intergenic
TGTGAGGGTGGATAAGTGGG+TGG 0.589818 1.4:+33582698 None:intergenic
TCCTCATCTTTATACTCTAG+AGG 0.592738 1.4:-33581971 MS.gene43330:CDS
CTTGAATAGGAAGCATGCAA+AGG 0.593306 1.4:-33582166 MS.gene43330:CDS
TGCTATGAGACGTCGTGCAG+CGG 0.599661 1.4:+33582464 None:intergenic
CGGAGCTAATTCGACAGCTG+AGG 0.600874 1.4:-33581939 MS.gene43330:CDS
GGATGTATATGTTGAAGAGA+TGG 0.601004 1.4:+33582586 None:intergenic
GGAGCTAATTCGACAGCTGA+GGG 0.601087 1.4:-33581938 MS.gene43330:CDS
AAGGGAAGGTGAATTGTGGG+AGG 0.602260 1.4:+33582775 None:intergenic
TACGAGCGTTATAACGCTCG+TGG 0.604840 1.4:-33582252 MS.gene43330:CDS
TCCTCTAGAGTATAAAGATG+AGG 0.607557 1.4:+33581970 None:intergenic
TAGGAAGCATGCAAAGGTTG+TGG 0.615535 1.4:-33582160 MS.gene43330:CDS
GTTATTCGTATCTATTTGCG+AGG 0.616971 1.4:-33581903 MS.gene43330:CDS
ACAACCAGTCCAATTAACTC+TGG 0.617135 1.4:+33582005 None:intergenic
AAGTGAGGTTGGTGTTTGTG+AGG 0.617458 1.4:+33582646 None:intergenic
ATTTGCGAGGAAATTCTCGC+CGG 0.625281 1.4:-33581890 MS.gene43330:CDS
CAAGAATCCAAATTTACACA+AGG 0.644692 1.4:+33582102 None:intergenic
CTATATAGAGATTCTCTCTG+AGG 0.645639 1.4:-33582286 MS.gene43330:CDS
TGGTGTGGGTGGTGGCACGG+TGG 0.645982 1.4:+33582721 None:intergenic
TCATCAGCTATATCCATCAA+CGG 0.649200 1.4:+33581856 None:intergenic
AGAGAATGTTATGTTGCCGG+AGG 0.659698 1.4:-33582229 MS.gene43330:CDS
CCAACCCGAAAATCCTCGAA+CGG 0.667048 1.4:+33582204 None:intergenic
GGTTATGGAAGACACCGCCA+GGG 0.669186 1.4:+33582535 None:intergenic
TGAGTGATGGTGAAGCACGT+TGG 0.676915 1.4:+33582490 None:intergenic
ACGAATCGAAACGACAAGAG+CGG 0.691362 1.4:+33582381 None:intergenic
ACCTCCGTCGTATCCCCTGG+CGG 0.701974 1.4:-33582549 MS.gene43330:CDS
ACCGCCAGGGGATACGACGG+AGG 0.706931 1.4:+33582548 None:intergenic
CGTGCAGCGGAGATGAGTGA+TGG 0.712720 1.4:+33582477 None:intergenic
GTTATGGAAGACACCGCCAG+GGG 0.770600 1.4:+33582536 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACATTAACTGATTTTTAAAA+AGG + chr1.4:33582343-33582362 None:intergenic 15.0%
!!! CACAGTTTTTTATCTTGAAT+AGG - chr1.4:33582510-33582529 MS.gene43330:CDS 25.0%
AGAGAATCTCTATATAGCTA+GGG + chr1.4:33582400-33582419 None:intergenic 30.0%
ATAAGCTATGGAAGAAGTTT+CGG - chr1.4:33582561-33582580 MS.gene43330:CDS 30.0%
CAAGAATCCAAATTTACACA+AGG + chr1.4:33582590-33582609 None:intergenic 30.0%
GAGAAACTAAAGCAAAGTAT+TGG + chr1.4:33582277-33582296 None:intergenic 30.0%
!! AAAGCAAAGTATTGGTTAAG+TGG + chr1.4:33582269-33582288 None:intergenic 30.0%
CTATATAGAGATTCTCTCTG+AGG - chr1.4:33582403-33582422 MS.gene43330:CDS 35.0%
GAGAGAATCTCTATATAGCT+AGG + chr1.4:33582401-33582420 None:intergenic 35.0%
GGATGTATATGTTGAAGAGA+TGG + chr1.4:33582106-33582125 None:intergenic 35.0%
GTTATTCGTATCTATTTGCG+AGG - chr1.4:33582786-33582805 MS.gene43330:CDS 35.0%
TCATCAGCTATATCCATCAA+CGG + chr1.4:33582836-33582855 None:intergenic 35.0%
TCCTCATCTTTATACTCTAG+AGG - chr1.4:33582718-33582737 MS.gene43330:CDS 35.0%
TCGGATTCCTTGTGTAAATT+TGG - chr1.4:33582580-33582599 MS.gene43330:CDS 35.0%
TGGTGAGAGATTATAAGCTA+TGG - chr1.4:33582549-33582568 MS.gene43330:CDS 35.0%
TTACACTGACCAGAGTTAAT+TGG - chr1.4:33582675-33582694 MS.gene43330:CDS 35.0%
TTATAACGCTCGTAGAGATT+CGG + chr1.4:33582431-33582450 None:intergenic 35.0%
TTATGGGAAAAATTCTTCGC+CGG - chr1.4:33582074-33582093 MS.gene43330:CDS 35.0%
! GCGAAGAATTTTTCCCATAA+AGG + chr1.4:33582074-33582093 None:intergenic 35.0%
! TTTAGGTTTAGGGTTTGTGA+GGG + chr1.4:33582009-33582028 None:intergenic 35.0%
! TTTTCCGACTCTTTTGTCAA+TGG - chr1.4:33582628-33582647 MS.gene43330:CDS 35.0%
!! AGTTAATTGGACTGGTTGTT+GGG - chr1.4:33582688-33582707 MS.gene43330:CDS 35.0%
!! TCCTCTAGAGTATAAAGATG+AGG + chr1.4:33582722-33582741 None:intergenic 35.0%
!! TTGGTTTGGAAGAGATGAAA+CGG + chr1.4:33582178-33582197 None:intergenic 35.0%
!!! AGGAAAGCGATTTTAGGTTT+AGG + chr1.4:33582020-33582039 None:intergenic 35.0%
!!! GGAAAGCGATTTTAGGTTTA+GGG + chr1.4:33582019-33582038 None:intergenic 35.0%
!!! TAGCTGATGATGTCATTTTC+CGG - chr1.4:33582846-33582865 MS.gene43330:CDS 35.0%
!!! TTTTAGGTTTAGGGTTTGTG+AGG + chr1.4:33582010-33582029 None:intergenic 35.0%
ACAACCAGTCCAATTAACTC+TGG + chr1.4:33582687-33582706 None:intergenic 40.0%
AGAAAGGGAAGGTGAATTGT+GGG + chr1.4:33581920-33581939 None:intergenic 40.0%
AGAGCTAGAGAGACAAGAAT+TGG + chr1.4:33581852-33581871 None:intergenic 40.0%
GTCGGAAAATAATGCTCTTC+TGG + chr1.4:33582617-33582636 None:intergenic 40.0%
TAAAGGAGCAAAAGTGAGGT+TGG + chr1.4:33582057-33582076 None:intergenic 40.0%
TCCTCCATTGACAAAAGAGT+CGG + chr1.4:33582635-33582654 None:intergenic 40.0%
TGGAAGAGATGAAACGGTTA+TGG + chr1.4:33582172-33582191 None:intergenic 40.0%
TGGAGAGAATGTTATGTTGC+CGG - chr1.4:33582457-33582476 MS.gene43330:CDS 40.0%
TGTCAATGGAGGATCTGAAT+GGG - chr1.4:33582642-33582661 MS.gene43330:CDS 40.0%
TTGTCAATGGAGGATCTGAA+TGG - chr1.4:33582641-33582660 MS.gene43330:CDS 40.0%
! AGGAAGAGGAAAGCGATTTT+AGG + chr1.4:33582026-33582045 None:intergenic 40.0%
! CTTGAATAGGAAGCATGCAA+AGG - chr1.4:33582523-33582542 MS.gene43330:CDS 40.0%
! GTTGAAGAGATGGTTGTTTC+CGG + chr1.4:33582096-33582115 None:intergenic 40.0%
!! ACCTCACTTTTGCTCCTTTA+TGG - chr1.4:33582057-33582076 MS.gene43330:CDS 40.0%
!! CCTCACTTTTGCTCCTTTAT+GGG - chr1.4:33582058-33582077 MS.gene43330:CDS 40.0%
!! GAGTTAATTGGACTGGTTGT+TGG - chr1.4:33582687-33582706 MS.gene43330:CDS 40.0%
!!! AGCTGATGATGTCATTTTCC+GGG - chr1.4:33582847-33582866 MS.gene43330:CDS 40.0%
ACGAATCGAAACGACAAGAG+CGG + chr1.4:33582311-33582330 None:intergenic 45.0%
AGAGAATGTTATGTTGCCGG+AGG - chr1.4:33582460-33582479 MS.gene43330:CDS 45.0%
ATTTGCGAGGAAATTCTCGC+CGG - chr1.4:33582799-33582818 MS.gene43330:CDS 45.0%
CCCATAAAGGAGCAAAAGTG+AGG + chr1.4:33582061-33582080 None:intergenic 45.0%
CTGACCAGAGTTAATTGGAC+TGG - chr1.4:33582680-33582699 MS.gene43330:CDS 45.0%
GAATTGTGGGAGGAGTTGTT+GGG + chr1.4:33581907-33581926 None:intergenic 45.0%
GAGAAAGGGAAGGTGAATTG+TGG + chr1.4:33581921-33581940 None:intergenic 45.0%
GTTTGTGAGGGTGGATAAGT+GGG + chr1.4:33581997-33582016 None:intergenic 45.0%
TGAATTGTGGGAGGAGTTGT+TGG + chr1.4:33581908-33581927 None:intergenic 45.0%
! AGGTTCCGTTCGAGGATTTT+CGG - chr1.4:33582480-33582499 MS.gene43330:CDS 45.0%
! TAGGAAGCATGCAAAGGTTG+TGG - chr1.4:33582529-33582548 MS.gene43330:CDS 45.0%
! TCCGACTCTTTTGTCAATGG+AGG - chr1.4:33582631-33582650 MS.gene43330:CDS 45.0%
!! AAGTGAGGTTGGTGTTTGTG+AGG + chr1.4:33582046-33582065 None:intergenic 45.0%
!! AATTGGACTGGTTGTTGGGA+TGG - chr1.4:33582692-33582711 MS.gene43330:CDS 45.0%
AAATCCTCGAACGGAACCTC+CGG + chr1.4:33582479-33582498 None:intergenic 50.0%
AAGGGAAGGTGAATTGTGGG+AGG + chr1.4:33581917-33581936 None:intergenic 50.0%
ATCAACGGCGCAAGACATTC+CGG + chr1.4:33582821-33582840 None:intergenic 50.0%
CCAACCCGAAAATCCTCGAA+CGG + chr1.4:33582488-33582507 None:intergenic 50.0%
CTCAGCTGTCGAATTAGCTC+CGG + chr1.4:33582752-33582771 None:intergenic 50.0%
GGAGCTAATTCGACAGCTGA+GGG - chr1.4:33582751-33582770 MS.gene43330:CDS 50.0%
GGTTTGTGAGGGTGGATAAG+TGG + chr1.4:33581998-33582017 None:intergenic 50.0%
TACGAGCGTTATAACGCTCG+TGG - chr1.4:33582437-33582456 MS.gene43330:CDS 50.0%
! AGGTTTAGGGTTTGTGAGGG+TGG + chr1.4:33582006-33582025 None:intergenic 50.0%
! GAGTGATGGTGAAGCACGTT+GGG + chr1.4:33582201-33582220 None:intergenic 50.0%
! GGTTCCGTTCGAGGATTTTC+GGG - chr1.4:33582481-33582500 MS.gene43330:CDS 50.0%
! GGTTGGTGTTTGTGAGGAAG+AGG + chr1.4:33582040-33582059 None:intergenic 50.0%
! TGAGTGATGGTGAAGCACGT+TGG + chr1.4:33582202-33582221 None:intergenic 50.0%
!! TACTCTAGAGGAAGTGTCGC+CGG - chr1.4:33582730-33582749 MS.gene43330:CDS 50.0%
!! TGAAGCACGTTGGGTTGGTT+TGG + chr1.4:33582192-33582211 None:intergenic 50.0%
CGGAGCTAATTCGACAGCTG+AGG - chr1.4:33582750-33582769 MS.gene43330:CDS 55.0%
GAGGATCTGAATGGGTGCAC+TGG - chr1.4:33582650-33582669 MS.gene43330:CDS 55.0%
GATGGTGAAGCACGTTGGGT+TGG + chr1.4:33582197-33582216 None:intergenic 55.0%
GGAATGTCTTGCGCCGTTGA+TGG - chr1.4:33582820-33582839 MS.gene43330:CDS 55.0%
GGAGTTGTTGGGAGAGGAAG+AGG + chr1.4:33581896-33581915 None:intergenic 55.0%
GGTTATGGAAGACACCGCCA+GGG + chr1.4:33582157-33582176 None:intergenic 55.0%
GTTATGGAAGACACCGCCAG+GGG + chr1.4:33582156-33582175 None:intergenic 55.0%
TGCTATGAGACGTCGTGCAG+CGG + chr1.4:33582228-33582247 None:intergenic 55.0%
TGTGAGGGTGGATAAGTGGG+TGG + chr1.4:33581994-33582013 None:intergenic 55.0%
! GGATAAGTGGGTGGTGGTGT+GGG + chr1.4:33581985-33582004 None:intergenic 55.0%
! TGGATAAGTGGGTGGTGGTG+TGG + chr1.4:33581986-33582005 None:intergenic 55.0%
!!! CCGTTCGAGGATTTTCGGGT+TGG - chr1.4:33582485-33582504 MS.gene43330:CDS 55.0%
CGGTTATGGAAGACACCGCC+AGG + chr1.4:33582158-33582177 None:intergenic 60.0%
CGTGCAGCGGAGATGAGTGA+TGG + chr1.4:33582215-33582234 None:intergenic 60.0%
GAGGGTGGATAAGTGGGTGG+TGG + chr1.4:33581991-33582010 None:intergenic 60.0%
GGACGAGTGGGAGAGAGATG+AGG + chr1.4:33581875-33581894 None:intergenic 60.0%
GGGAGAGGAAGAGGACGAGT+GGG + chr1.4:33581887-33581906 None:intergenic 60.0%
GTGGAAGAGGGCCAGGTCAT+CGG + chr1.4:33581952-33581971 None:intergenic 60.0%
GTGGGAGGAGTTGTTGGGAG+AGG + chr1.4:33581902-33581921 None:intergenic 60.0%
TGGGAGAGGAAGAGGACGAG+TGG + chr1.4:33581888-33581907 None:intergenic 60.0%
! TAAGTGGGTGGTGGTGTGGG+TGG + chr1.4:33581982-33582001 None:intergenic 60.0%
ACCACCTCCGTCGTATCCCC+TGG - chr1.4:33582137-33582156 MS.gene43330:CDS 65.0%
ACCTCCGTCGTATCCCCTGG+CGG - chr1.4:33582140-33582159 MS.gene43330:CDS 65.0%
AGGGGATACGACGGAGGTGG+TGG + chr1.4:33582138-33582157 None:intergenic 65.0%
GACACCGCCAGGGGATACGA+CGG + chr1.4:33582147-33582166 None:intergenic 65.0%
GGGATACGACGGAGGTGGTG+GGG + chr1.4:33582136-33582155 None:intergenic 65.0%
GGGGATACGACGGAGGTGGT+GGG + chr1.4:33582137-33582156 None:intergenic 65.0%
GTTGCCGGAGGTTCCGTTCG+AGG - chr1.4:33582472-33582491 MS.gene43330:CDS 65.0%
! CATCGGCGTCGGGGGAGAAA+GGG + chr1.4:33581935-33581954 None:intergenic 65.0%
! GGTGGTGGCACGGTGGAAGA+GGG + chr1.4:33581964-33581983 None:intergenic 65.0%
! TCATCGGCGTCGGGGGAGAA+AGG + chr1.4:33581936-33581955 None:intergenic 65.0%
! TGGTGGTGTGGGTGGTGGCA+CGG + chr1.4:33581974-33581993 None:intergenic 65.0%
ACCGCCAGGGGATACGACGG+AGG + chr1.4:33582144-33582163 None:intergenic 70.0%
AGGGCCAGGTCATCGGCGTC+GGG + chr1.4:33581945-33581964 None:intergenic 70.0%
GAGGGCCAGGTCATCGGCGT+CGG + chr1.4:33581946-33581965 None:intergenic 70.0%
GCCAGGGGATACGACGGAGG+TGG + chr1.4:33582141-33582160 None:intergenic 70.0%
TGGCACGGTGGAAGAGGGCC+AGG + chr1.4:33581959-33581978 None:intergenic 70.0%
! GGCGTCGGGGGAGAAAGGGA+AGG + chr1.4:33581931-33581950 None:intergenic 70.0%
! GGGTGGTGGCACGGTGGAAG+AGG + chr1.4:33581965-33581984 None:intergenic 70.0%
! GTGGGTGGTGGTGTGGGTGG+TGG + chr1.4:33581979-33581998 None:intergenic 70.0%
! TGGTGTGGGTGGTGGCACGG+TGG + chr1.4:33581971-33581990 None:intergenic 70.0%
!! CGGAGGTGGTGGGGTCTGCA+TGG + chr1.4:33582127-33582146 None:intergenic 70.0%
CTCCCCCGACGCCGATGACC+TGG - chr1.4:33581938-33581957 MS.gene43330:CDS 75.0%
GGGCCAGGTCATCGGCGTCG+GGG + chr1.4:33581944-33581963 None:intergenic 75.0%
! GGCCAGGTCATCGGCGTCGG+GGG + chr1.4:33581943-33581962 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 33581837 33582874 33581837 ID=MS.gene43330
chr1.4 mRNA 33581837 33582874 33581837 ID=MS.gene43330.t1;Parent=MS.gene43330
chr1.4 exon 33581837 33582874 33581837 ID=MS.gene43330.t1.exon1;Parent=MS.gene43330.t1
chr1.4 CDS 33581837 33582874 33581837 ID=cds.MS.gene43330.t1;Parent=MS.gene43330.t1
Gene Sequence

>MS.gene43330

ATGTTGTCATCACCAATTCTTGTCTCTCTAGCTCTCCTCATCTCTCTCCCACTCGTCCTCTTCCTCTCCCAACAACTCCTCCCACAATTCACCTTCCCTTTCTCCCCCGACGCCGATGACCTGGCCCTCTTCCACCGTGCCACCACCCACACCACCACCCACTTATCCACCCTCACAAACCCTAAACCTAAAATCGCTTTCCTCTTCCTCACAAACACCAACCTCACTTTTGCTCCTTTATGGGAAAAATTCTTCGCCGGAAACAACCATCTCTTCAACATATACATCCATGCAGACCCCACCACCTCCGTCGTATCCCCTGGCGGTGTCTTCCATAACCGTTTCATCTCTTCCAAACCAACCCAACGTGCTTCACCATCACTCATCTCCGCTGCACGACGTCTCATAGCATCTGCTCTCTTAGATGATCCACTTAACCAATACTTTGCTTTAGTTTCTCAACACTGTGTTCCGCTCTTGTCGTTTCGATTCGTTTATAACTACCTTTTTAAAAATCAGTTAATGTCGTTAGCGAGTTTTTCTGACTTTAACTTACTGTACCCTAGCTATATAGAGATTCTCTCTGAGGATCCGAATCTCTACGAGCGTTATAACGCTCGTGGAGAGAATGTTATGTTGCCGGAGGTTCCGTTCGAGGATTTTCGGGTTGGTTCACAGTTTTTTATCTTGAATAGGAAGCATGCAAAGGTTGTGGTGAGAGATTATAAGCTATGGAAGAAGTTTCGGATTCCTTGTGTAAATTTGGATTCTTGTTATCCAGAAGAGCATTATTTTCCGACTCTTTTGTCAATGGAGGATCTGAATGGGTGCACTGGCTTTACACTGACCAGAGTTAATTGGACTGGTTGTTGGGATGGACATCCTCATCTTTATACTCTAGAGGAAGTGTCGCCGGAGCTAATTCGACAGCTGAGGGTGTCGAATTCGAGTTATTCGTATCTATTTGCGAGGAAATTCTCGCCGGAATGTCTTGCGCCGTTGATGGATATAGCTGATGATGTCATTTTCCGGGATTGA
Protein sequence

>MS.gene43330.t1

MLSSPILVSLALLISLPLVLFLSQQLLPQFTFPFSPDADDLALFHRATTHTTTHLSTLTNPKPKIAFLFLTNTNLTFAPLWEKFFAGNNHLFNIYIHADPTTSVVSPGGVFHNRFISSKPTQRASPSLISAARRLIASALLDDPLNQYFALVSQHCVPLLSFRFVYNYLFKNQLMSLASFSDFNLLYPSYIEILSEDPNLYERYNARGENVMLPEVPFEDFRVGSQFFILNRKHAKVVVRDYKLWKKFRIPCVNLDSCYPEEHYFPTLLSMEDLNGCTGFTLTRVNWTGCWDGHPHLYTLEEVSPELIRQLRVSNSSYSYLFARKFSPECLAPLMDIADDVIFRD