AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35522

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35522.t1 MTR_2g087610 98.098 368 7 0 1 368 1 368 0.0 736
MS.gene35522.t1 MTR_1g048320 50.820 366 146 9 7 368 9 344 4.91e-105 313
MS.gene35522.t1 MTR_1g031080 61.864 236 86 1 133 368 61 292 3.81e-101 301
MS.gene35522.t1 MTR_1g031060 56.757 296 115 4 75 368 78 362 2.23e-98 296
MS.gene35522.t1 MTR_3g106930 47.419 310 117 4 78 368 95 377 8.42e-88 270
MS.gene35522.t1 MTR_1g110290 35.216 301 148 11 78 365 125 391 2.15e-41 150
MS.gene35522.t1 MTR_2g037600 30.915 317 155 11 75 360 103 386 3.21e-39 144
MS.gene35522.t1 MTR_8g015500 34.564 298 155 10 78 365 144 411 3.75e-39 144
MS.gene35522.t1 MTR_3g108740 31.629 313 148 11 78 358 93 371 2.97e-38 141
MS.gene35522.t1 MTR_1g009960 30.650 323 160 11 66 358 102 390 8.17e-38 140
MS.gene35522.t1 MTR_5g055480 34.448 299 152 11 78 365 123 388 8.59e-38 140
MS.gene35522.t1 MTR_1g016880 33.333 300 157 10 78 366 107 374 2.27e-37 138
MS.gene35522.t1 MTR_2g005350 32.194 351 186 11 34 365 119 436 3.60e-37 139
MS.gene35522.t1 MTR_1g009960 32.609 276 145 10 66 334 102 343 7.76e-37 137
MS.gene35522.t1 MTR_4g127550 29.677 310 157 10 78 358 108 385 1.93e-36 136
MS.gene35522.t1 MTR_6g083830 32.886 298 160 10 78 365 150 417 3.98e-36 136
MS.gene35522.t1 MTR_2g037620 30.094 319 156 12 73 360 95 377 6.89e-36 135
MS.gene35522.t1 MTR_5g006930 32.653 294 158 9 78 361 117 380 1.10e-35 134
MS.gene35522.t1 MTR_5g006920 31.987 297 164 9 78 365 152 419 1.75e-33 129
MS.gene35522.t1 MTR_2g024120 31.288 326 153 9 78 359 62 360 1.16e-32 125
MS.gene35522.t1 MTR_3g104840 31.525 295 150 8 74 361 138 387 1.76e-31 123
MS.gene35522.t1 MTR_8g094180 29.721 323 148 10 78 349 56 350 2.71e-24 102
MS.gene35522.t1 MTR_8g094180 30.851 282 139 9 78 331 56 309 5.61e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35522.t1 AT5G22070 61.930 373 126 5 1 368 1 362 5.28e-162 458
MS.gene35522.t1 AT3G52060 54.655 333 127 5 36 368 38 346 1.55e-120 352
MS.gene35522.t1 AT5G25330 48.684 304 121 6 78 368 81 362 8.20e-87 267
MS.gene35522.t1 AT4G32290 43.733 375 141 9 36 368 33 379 8.24e-83 257
MS.gene35522.t1 AT4G31350 33.968 315 151 10 71 358 82 366 4.57e-45 159
MS.gene35522.t1 AT4G31350 33.968 315 151 10 71 358 82 366 4.57e-45 159
MS.gene35522.t1 AT4G31350 33.968 315 151 10 71 358 82 366 4.57e-45 159
MS.gene35522.t1 AT4G31350 33.968 315 146 11 71 358 82 361 3.58e-44 156
MS.gene35522.t1 AT3G21310 33.784 296 159 9 78 365 115 381 7.54e-42 150
MS.gene35522.t1 AT3G21310 33.784 296 159 9 78 365 115 381 7.54e-42 150
MS.gene35522.t1 AT1G51770 35.235 298 152 11 78 365 124 390 1.22e-41 150
MS.gene35522.t1 AT5G25970 36.054 294 147 11 78 361 119 381 6.68e-41 148
MS.gene35522.t1 AT5G25970 36.054 294 147 11 78 361 119 381 6.68e-41 148
MS.gene35522.t1 AT5G25970 36.054 294 147 11 78 361 119 381 6.68e-41 148
MS.gene35522.t1 AT1G10280 33.775 302 152 12 78 365 143 410 8.55e-41 148
MS.gene35522.t1 AT4G25870 32.803 314 148 12 78 360 99 380 6.90e-39 143
MS.gene35522.t1 AT2G19160 32.797 311 147 11 78 358 106 384 1.49e-38 142
MS.gene35522.t1 AT2G19160 32.797 311 147 11 78 358 106 384 1.49e-38 142
MS.gene35522.t1 AT4G30060 30.769 312 156 9 78 360 109 389 3.27e-37 139
MS.gene35522.t1 AT4G30060 30.769 312 156 9 78 360 109 389 3.27e-37 139
MS.gene35522.t1 AT5G57270 33.969 262 132 10 78 331 98 326 5.31e-37 138
MS.gene35522.t1 AT5G57270 33.969 262 132 10 78 331 98 326 5.31e-37 138
MS.gene35522.t1 AT5G57270 33.969 262 132 10 78 331 98 326 5.31e-37 138
MS.gene35522.t1 AT5G57270 33.969 262 132 10 78 331 98 326 5.31e-37 138
MS.gene35522.t1 AT5G57270 33.969 262 132 10 78 331 98 326 5.31e-37 138
MS.gene35522.t1 AT1G68390 33.775 302 153 13 78 365 138 406 1.11e-36 137
MS.gene35522.t1 AT5G11730 32.886 298 159 10 78 365 118 384 2.52e-36 136
MS.gene35522.t1 AT5G16170 31.410 312 168 10 69 365 127 407 2.48e-35 134
MS.gene35522.t1 AT1G10880 30.395 329 175 11 43 361 96 380 4.53e-34 130
MS.gene35522.t1 AT1G68380 33.113 302 155 11 78 366 124 391 1.37e-32 126
MS.gene35522.t1 AT1G11940 31.034 348 168 10 48 352 46 364 1.54e-30 120
MS.gene35522.t1 AT1G62305 31.832 333 154 12 78 365 69 373 5.35e-30 119
MS.gene35522.t1 AT1G51770 31.544 298 136 11 78 365 124 363 1.17e-29 117
MS.gene35522.t1 AT5G14550 31.802 283 154 9 78 347 65 321 4.59e-29 115
MS.gene35522.t1 AT5G14550 30.696 316 149 11 78 349 65 354 3.26e-27 111
MS.gene35522.t1 AT5G14550 30.599 317 150 11 78 350 65 355 4.31e-27 110
MS.gene35522.t1 AT1G73810 31.250 304 163 12 76 365 145 416 3.92e-26 108
MS.gene35522.t1 AT1G62305 34.468 235 108 7 78 294 69 275 2.50e-25 104
MS.gene35522.t1 AT1G11940 32.759 232 125 5 48 277 46 248 8.96e-23 96.7
MS.gene35522.t1 AT5G14550 29.900 301 146 11 78 350 65 328 1.37e-21 94.7
MS.gene35522.t1 AT3G21310 32.099 162 84 5 78 239 115 250 2.36e-15 75.9

Find 111 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGAAAGTTAGAAGATA+TGG 0.207439 2.4:+13230663 None:intergenic
GGAAGAGAGATGAAAGAATT+TGG 0.208741 2.4:+13230482 None:intergenic
GACATTGTGGAGGAAGTTTA+AGG 0.229533 2.4:-13229881 MS.gene35522:CDS
ATCTTTGAAAATAACAGATT+TGG 0.276902 2.4:+13229594 None:intergenic
ACATGCTTTGGTTGTTGTTA+AGG 0.308207 2.4:-13229908 MS.gene35522:CDS
TAAACATTGAATAATTTAGA+TGG 0.309104 2.4:+13230369 None:intergenic
GAGGATTCTGATGGTGTTAC+TGG 0.315065 2.4:-13229793 MS.gene35522:CDS
AGTTCATCTGTTGGGGAGAT+TGG 0.319569 2.4:+13230573 None:intergenic
AATTGGACTGGGACTGTTAA+TGG 0.353305 2.4:-13229751 MS.gene35522:CDS
ATTTCAACCGAACACCGAAT+TGG 0.361586 2.4:+13230070 None:intergenic
ATACACTTACCAATGTGAAT+TGG 0.369149 2.4:-13229768 MS.gene35522:CDS
TGTTGGGGAGATTGGGATGG+GGG 0.394042 2.4:+13230581 None:intergenic
GAGAGAAAGTTAGAAGATAT+GGG 0.398505 2.4:+13230664 None:intergenic
TTTCAACCGAACACCGAATT+GGG 0.399194 2.4:+13230071 None:intergenic
TGTCATCGAGTTCATCTGTT+GGG 0.402532 2.4:+13230565 None:intergenic
GAAATTTGTTCCTGATTGCT+TGG 0.406788 2.4:-13229635 MS.gene35522:CDS
TACGTTTGATTTAACCGATT+CGG 0.406838 2.4:-13230100 MS.gene35522:CDS
GATTCCAGAGAGGGGTGAAA+TGG 0.407050 2.4:+13230409 None:intergenic
CGTTTGAGAAATTTCGTGTT+GGG 0.409497 2.4:-13229957 MS.gene35522:CDS
GGTTGTAACTGTTGCTTAAA+CGG 0.411781 2.4:+13230310 None:intergenic
TCAACAATGGACCTAGGTTA+TGG 0.412678 2.4:-13230026 MS.gene35522:CDS
TCTGTTGGGGAGATTGGGAT+GGG 0.413320 2.4:+13230579 None:intergenic
AATCGACCCAATTCGGTGTT+CGG 0.414363 2.4:-13230077 MS.gene35522:CDS
GGAGCGAGAAAGAAGAGAAT+TGG 0.415517 2.4:+13230627 None:intergenic
GATTCGGAATCGACCCAATT+CGG 0.415724 2.4:-13230084 MS.gene35522:CDS
GTTCATCTGTTGGGGAGATT+GGG 0.416853 2.4:+13230574 None:intergenic
TTTGACAAGAAAACATGCTT+TGG 0.419279 2.4:-13229920 MS.gene35522:CDS
TGATTGCTTGGAACCATTGA+TGG 0.426338 2.4:-13229623 MS.gene35522:CDS
ATCTGTTGGGGAGATTGGGA+TGG 0.426551 2.4:+13230578 None:intergenic
AATTTCTCAAACGGAACTTC+CGG 0.431140 2.4:+13229967 None:intergenic
AGGGGTGTGAAGAGGAAGAA+GGG 0.436253 2.4:+13230505 None:intergenic
ATCTCCATTTCACCCCTCTC+TGG 0.436718 2.4:-13230413 MS.gene35522:CDS
ATCAATGGTTCCAAGCAATC+AGG 0.437224 2.4:+13229625 None:intergenic
GCAATCAGGAACAAATTTCC+TGG 0.437708 2.4:+13229639 None:intergenic
CTGTTGGGGAGATTGGGATG+GGG 0.455104 2.4:+13230580 None:intergenic
AAACATTGAATAATTTAGAT+GGG 0.466588 2.4:+13230370 None:intergenic
GGATGAGGTGGGAGAATAGC+AGG 0.466807 2.4:+13230606 None:intergenic
GTTGAGAAAGTCGAATAATT+CGG 0.467142 2.4:-13229677 MS.gene35522:CDS
GACGGTACAAATTTGAAGAT+GGG 0.469772 2.4:+13230288 None:intergenic
GAGGGGTGTGAAGAGGAAGA+AGG 0.470582 2.4:+13230504 None:intergenic
AGACGGTACAAATTTGAAGA+TGG 0.472395 2.4:+13230287 None:intergenic
CCGTTTGAGAAATTTCGTGT+TGG 0.473342 2.4:-13229958 MS.gene35522:CDS
GGGTGTGAAGAGGAAGAAGG+GGG 0.474839 2.4:+13230507 None:intergenic
GGGGTGTGAAGAGGAAGAAG+GGG 0.477919 2.4:+13230506 None:intergenic
CTTACCAATGTGAATTGGAC+TGG 0.493929 2.4:-13229763 MS.gene35522:CDS
AATTATTCGACTTTCTCAAC+CGG 0.502661 2.4:+13229680 None:intergenic
GATCGGTGTTTGTGAGGAAA+AGG 0.508484 2.4:+13230433 None:intergenic
GATTTGGCAATACCCATCAA+TGG 0.512678 2.4:+13229610 None:intergenic
GGATCGCAGAAGCAAGTAAA+CGG 0.513510 2.4:+13230220 None:intergenic
TCCTACGTTGTTGTCTATGG+AGG 0.514638 2.4:-13229812 MS.gene35522:CDS
GTAGGAAAATAATGTTCCTC+AGG 0.514706 2.4:+13229829 None:intergenic
TCCTCCGGCTGATACGTATG+AGG 0.524726 2.4:+13229724 None:intergenic
CTTTGGTTGTTGTTAAGGAT+AGG 0.527282 2.4:-13229903 MS.gene35522:CDS
CGCGTGGCGGAGATTAAAGT+GGG 0.528482 2.4:+13230243 None:intergenic
GGTTAACGCGCGGGTCGGAA+TGG 0.528505 2.4:+13230343 None:intergenic
GCGCGTGGCGGAGATTAAAG+TGG 0.534851 2.4:+13230242 None:intergenic
GAGAGATGAAAGAATTTGGA+GGG 0.534851 2.4:+13230486 None:intergenic
AGAGAGATGAAAGAATTTGG+AGG 0.536586 2.4:+13230485 None:intergenic
AAGAACGATGAAATAAGCAT+TGG 0.538889 2.4:+13230185 None:intergenic
TTGTTAAGGATAGGACATTG+TGG 0.540935 2.4:-13229894 MS.gene35522:CDS
GAATTTGGAGGGGTGTGAAG+AGG 0.541017 2.4:+13230497 None:intergenic
CACTCATCATCACGGTAACA+AGG 0.544806 2.4:+13229856 None:intergenic
CCAACACGAAATTTCTCAAA+CGG 0.545393 2.4:+13229958 None:intergenic
TGGACGTTACGCGATGATGC+CGG 0.547095 2.4:-13229986 MS.gene35522:CDS
TATAATCAAACGAATGAAGA+GGG 0.552046 2.4:+13230148 None:intergenic
GGCGCGTGAGGTTAACGCGC+GGG 0.553598 2.4:+13230334 None:intergenic
TTTATTGAAATTATCAACAA+TGG 0.555157 2.4:-13230039 MS.gene35522:CDS
TGCTTAAACGGAGGCGCGTG+AGG 0.557222 2.4:+13230322 None:intergenic
GAATTGGGTCGATTCCGAAT+CGG 0.559523 2.4:+13230086 None:intergenic
AACATTGAATAATTTAGATG+GGG 0.564738 2.4:+13230371 None:intergenic
GTGAAATAGCGGTGTTGAAG+AGG 0.564970 2.4:+13230538 None:intergenic
GAAAGAAGAGATTCCAGAGA+GGG 0.567482 2.4:+13230400 None:intergenic
TTGTCTATGGAGGATTCTGA+TGG 0.567511 2.4:-13229802 MS.gene35522:CDS
CCATCCTCATACGTATCAGC+CGG 0.571067 2.4:-13229728 MS.gene35522:CDS
ACGGTACAAATTTGAAGATG+GGG 0.571190 2.4:+13230289 None:intergenic
CGTGAGGTTAACGCGCGGGT+CGG 0.572231 2.4:+13230338 None:intergenic
ATGTCATCGAGTTCATCTGT+TGG 0.585625 2.4:+13230564 None:intergenic
GATTGCTTGGAACCATTGAT+GGG 0.586536 2.4:-13229622 MS.gene35522:CDS
AGAGATGAAAGAATTTGGAG+GGG 0.587496 2.4:+13230487 None:intergenic
TGTAACTGTTGCTTAAACGG+AGG 0.591767 2.4:+13230313 None:intergenic
AGAAGCAAGTAAACGGCGCG+TGG 0.594303 2.4:+13230227 None:intergenic
TGAAAGAAGAGATTCCAGAG+AGG 0.594543 2.4:+13230399 None:intergenic
AGCTCCGGTGACACTTCCTC+CGG 0.595229 2.4:+13229709 None:intergenic
CTCAACCGGAGAATAAGCTC+CGG 0.595442 2.4:+13229694 None:intergenic
AGAGAATTGGAAGAGAGAGA+AGG 0.595952 2.4:+13230640 None:intergenic
TGGAAGCGTTACACGGCGCG+TGG 0.597375 2.4:-13230006 MS.gene35522:CDS
CCGGCTGATACGTATGAGGA+TGG 0.599049 2.4:+13229728 None:intergenic
GAGAGGGGTGAAATGGAGAT+CGG 0.601775 2.4:+13230416 None:intergenic
AAATTATCAACAATGGACCT+AGG 0.602503 2.4:-13230032 MS.gene35522:CDS
TCAGCCGGAGGAAGTGTCAC+CGG 0.606036 2.4:-13229713 MS.gene35522:CDS
TGTCACCGGAGCTTATTCTC+CGG 0.607381 2.4:-13229699 MS.gene35522:CDS
AGCAAGTAAACGGCGCGTGG+CGG 0.613336 2.4:+13230230 None:intergenic
ATATAATCAAACGAATGAAG+AGG 0.616226 2.4:+13230147 None:intergenic
TTACCAATGTGAATTGGACT+GGG 0.620634 2.4:-13229762 MS.gene35522:CDS
AATGGAGATCGGTGTTTGTG+AGG 0.622749 2.4:+13230427 None:intergenic
GTCATCGAGTTCATCTGTTG+GGG 0.628284 2.4:+13230566 None:intergenic
GGGTGTAGAATGTGAAATAG+CGG 0.628873 2.4:+13230527 None:intergenic
GTGTAACGCTTCCATAACCT+AGG 0.638855 2.4:+13230015 None:intergenic
TAGGTTATGGAAGCGTTACA+CGG 0.639248 2.4:-13230013 MS.gene35522:CDS
AAAGAAGAGATTCCAGAGAG+GGG 0.653261 2.4:+13230401 None:intergenic
TCCTCATACGTATCAGCCGG+AGG 0.654016 2.4:-13229725 MS.gene35522:CDS
AGGCGCGTGAGGTTAACGCG+CGG 0.654886 2.4:+13230333 None:intergenic
TTAAGGATAGGACATTGTGG+AGG 0.656863 2.4:-13229891 MS.gene35522:CDS
AGTCCCAGTCCAATTCACAT+TGG 0.674151 2.4:+13229759 None:intergenic
CAGGATAACACTCATCATCA+CGG 0.676655 2.4:+13229848 None:intergenic
CGTGGCGGAGATTAAAGTGG+GGG 0.678137 2.4:+13230245 None:intergenic
GAATCGGTTAAATCAAACGT+AGG 0.693798 2.4:+13230102 None:intergenic
AAGCATTGGAAGCATCGTCG+AGG 0.706280 2.4:+13230199 None:intergenic
GCGTGGCGGAGATTAAAGTG+GGG 0.740739 2.4:+13230244 None:intergenic
TCCTCCATAGACAACAACGT+AGG 0.744754 2.4:+13229811 None:intergenic
TGATGATGAGTGTTATCCTG+AGG 0.777255 2.4:-13229845 MS.gene35522:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATTGAATAATTTAGAT+GGG + chr2.4:13229899-13229918 None:intergenic 15.0%
!! TAAACATTGAATAATTTAGA+TGG + chr2.4:13229900-13229919 None:intergenic 15.0%
!! TTTATTGAAATTATCAACAA+TGG - chr2.4:13230227-13230246 MS.gene35522:CDS 15.0%
!! AACATTGAATAATTTAGATG+GGG + chr2.4:13229898-13229917 None:intergenic 20.0%
!!! ATCTTTGAAAATAACAGATT+TGG + chr2.4:13230675-13230694 None:intergenic 20.0%
! ATATAATCAAACGAATGAAG+AGG + chr2.4:13230122-13230141 None:intergenic 25.0%
! TATAATCAAACGAATGAAGA+GGG + chr2.4:13230121-13230140 None:intergenic 25.0%
AAATTATCAACAATGGACCT+AGG - chr2.4:13230234-13230253 MS.gene35522:CDS 30.0%
AAGAACGATGAAATAAGCAT+TGG + chr2.4:13230084-13230103 None:intergenic 30.0%
AATTATTCGACTTTCTCAAC+CGG + chr2.4:13230589-13230608 None:intergenic 30.0%
AGAGAGAAAGTTAGAAGATA+TGG + chr2.4:13229606-13229625 None:intergenic 30.0%
ATACACTTACCAATGTGAAT+TGG - chr2.4:13230498-13230517 MS.gene35522:CDS 30.0%
GAGAGAAAGTTAGAAGATAT+GGG + chr2.4:13229605-13229624 None:intergenic 30.0%
GTTGAGAAAGTCGAATAATT+CGG - chr2.4:13230589-13230608 MS.gene35522:CDS 30.0%
TTTGACAAGAAAACATGCTT+TGG - chr2.4:13230346-13230365 MS.gene35522:CDS 30.0%
! GAAAAGGAAAGCAATTTTGA+AGG + chr2.4:13229820-13229839 None:intergenic 30.0%
! TACGTTTGATTTAACCGATT+CGG - chr2.4:13230166-13230185 MS.gene35522:CDS 30.0%
!! AATTTTGAAGGTAGGGTTTT+TGG + chr2.4:13229808-13229827 None:intergenic 30.0%
AATTTCTCAAACGGAACTTC+CGG + chr2.4:13230302-13230321 None:intergenic 35.0%
ACGGTACAAATTTGAAGATG+GGG + chr2.4:13229980-13229999 None:intergenic 35.0%
AGACGGTACAAATTTGAAGA+TGG + chr2.4:13229982-13230001 None:intergenic 35.0%
AGAGAGATGAAAGAATTTGG+AGG + chr2.4:13229784-13229803 None:intergenic 35.0%
AGAGATGAAAGAATTTGGAG+GGG + chr2.4:13229782-13229801 None:intergenic 35.0%
CCAACACGAAATTTCTCAAA+CGG + chr2.4:13230311-13230330 None:intergenic 35.0%
CGTTTGAGAAATTTCGTGTT+GGG - chr2.4:13230309-13230328 MS.gene35522:CDS 35.0%
GAATCGGTTAAATCAAACGT+AGG + chr2.4:13230167-13230186 None:intergenic 35.0%
GACGGTACAAATTTGAAGAT+GGG + chr2.4:13229981-13230000 None:intergenic 35.0%
GAGAGATGAAAGAATTTGGA+GGG + chr2.4:13229783-13229802 None:intergenic 35.0%
GGAAGAGAGATGAAAGAATT+TGG + chr2.4:13229787-13229806 None:intergenic 35.0%
GTAGGAAAATAATGTTCCTC+AGG + chr2.4:13230440-13230459 None:intergenic 35.0%
TTACCAATGTGAATTGGACT+GGG - chr2.4:13230504-13230523 MS.gene35522:CDS 35.0%
TTGTTAAGGATAGGACATTG+TGG - chr2.4:13230372-13230391 MS.gene35522:CDS 35.0%
! ACATGCTTTGGTTGTTGTTA+AGG - chr2.4:13230358-13230377 MS.gene35522:CDS 35.0%
! CTTTGGTTGTTGTTAAGGAT+AGG - chr2.4:13230363-13230382 MS.gene35522:CDS 35.0%
! GAAATTTGTTCCTGATTGCT+TGG - chr2.4:13230631-13230650 MS.gene35522:CDS 35.0%
! GGTTGTAACTGTTGCTTAAA+CGG + chr2.4:13229959-13229978 None:intergenic 35.0%
! TTTTCCTACGTTGTTGTCTA+TGG - chr2.4:13230451-13230470 MS.gene35522:CDS 35.0%
!! AGGAAAGCAATTTTGAAGGT+AGG + chr2.4:13229816-13229835 None:intergenic 35.0%
!! GGAAAGCAATTTTGAAGGTA+GGG + chr2.4:13229815-13229834 None:intergenic 35.0%
AAAGAAGAGATTCCAGAGAG+GGG + chr2.4:13229868-13229887 None:intergenic 40.0%
AGAGAATTGGAAGAGAGAGA+AGG + chr2.4:13229629-13229648 None:intergenic 40.0%
ATCAATGGTTCCAAGCAATC+AGG + chr2.4:13230644-13230663 None:intergenic 40.0%
ATGTCATCGAGTTCATCTGT+TGG + chr2.4:13229705-13229724 None:intergenic 40.0%
ATTTCAACCGAACACCGAAT+TGG + chr2.4:13230199-13230218 None:intergenic 40.0%
CAGGATAACACTCATCATCA+CGG + chr2.4:13230421-13230440 None:intergenic 40.0%
CCGTTTGAGAAATTTCGTGT+TGG - chr2.4:13230308-13230327 MS.gene35522:CDS 40.0%
CTTACCAATGTGAATTGGAC+TGG - chr2.4:13230503-13230522 MS.gene35522:CDS 40.0%
GAAAGAAGAGATTCCAGAGA+GGG + chr2.4:13229869-13229888 None:intergenic 40.0%
GACATTGTGGAGGAAGTTTA+AGG - chr2.4:13230385-13230404 MS.gene35522:CDS 40.0%
GATTGCTTGGAACCATTGAT+GGG - chr2.4:13230644-13230663 MS.gene35522:CDS 40.0%
GATTTGGCAATACCCATCAA+TGG + chr2.4:13230659-13230678 None:intergenic 40.0%
GCAATCAGGAACAAATTTCC+TGG + chr2.4:13230630-13230649 None:intergenic 40.0%
GGGTGTAGAATGTGAAATAG+CGG + chr2.4:13229742-13229761 None:intergenic 40.0%
TCAACAATGGACCTAGGTTA+TGG - chr2.4:13230240-13230259 MS.gene35522:CDS 40.0%
TGAAAGAAGAGATTCCAGAG+AGG + chr2.4:13229870-13229889 None:intergenic 40.0%
TGATTGCTTGGAACCATTGA+TGG - chr2.4:13230643-13230662 MS.gene35522:CDS 40.0%
TGTCATCGAGTTCATCTGTT+GGG + chr2.4:13229704-13229723 None:intergenic 40.0%
TTAAGGATAGGACATTGTGG+AGG - chr2.4:13230375-13230394 MS.gene35522:CDS 40.0%
TTGTCTATGGAGGATTCTGA+TGG - chr2.4:13230464-13230483 MS.gene35522:CDS 40.0%
TTTCAACCGAACACCGAATT+GGG + chr2.4:13230198-13230217 None:intergenic 40.0%
! AATTGGACTGGGACTGTTAA+TGG - chr2.4:13230515-13230534 MS.gene35522:CDS 40.0%
! TGATGATGAGTGTTATCCTG+AGG - chr2.4:13230421-13230440 MS.gene35522:CDS 40.0%
! TGTAACTGTTGCTTAAACGG+AGG + chr2.4:13229956-13229975 None:intergenic 40.0%
!! TAGGTTATGGAAGCGTTACA+CGG - chr2.4:13230253-13230272 MS.gene35522:CDS 40.0%
AGATTAAAGTGGGGGAAGCA+CGG + chr2.4:13230016-13230035 None:intergenic 45.0%
AGTCCCAGTCCAATTCACAT+TGG + chr2.4:13230510-13230529 None:intergenic 45.0%
AGTTCATCTGTTGGGGAGAT+TGG + chr2.4:13229696-13229715 None:intergenic 45.0%
CACTCATCATCACGGTAACA+AGG + chr2.4:13230413-13230432 None:intergenic 45.0%
GATTCGGAATCGACCCAATT+CGG - chr2.4:13230182-13230201 MS.gene35522:CDS 45.0%
GGAGCGAGAAAGAAGAGAAT+TGG + chr2.4:13229642-13229661 None:intergenic 45.0%
GGATCGCAGAAGCAAGTAAA+CGG + chr2.4:13230049-13230068 None:intergenic 45.0%
GTCATCGAGTTCATCTGTTG+GGG + chr2.4:13229703-13229722 None:intergenic 45.0%
GTGTAACGCTTCCATAACCT+AGG + chr2.4:13230254-13230273 None:intergenic 45.0%
GTTCATCTGTTGGGGAGATT+GGG + chr2.4:13229695-13229714 None:intergenic 45.0%
TCCTACGTTGTTGTCTATGG+AGG - chr2.4:13230454-13230473 MS.gene35522:CDS 45.0%
TCCTCCATAGACAACAACGT+AGG + chr2.4:13230458-13230477 None:intergenic 45.0%
! AATGGAGATCGGTGTTTGTG+AGG + chr2.4:13229842-13229861 None:intergenic 45.0%
! GAATTGGGTCGATTCCGAAT+CGG + chr2.4:13230183-13230202 None:intergenic 45.0%
! GATCGGTGTTTGTGAGGAAA+AGG + chr2.4:13229836-13229855 None:intergenic 45.0%
! GCACGGTAGGTTTTCTTAGA+CGG + chr2.4:13229999-13230018 None:intergenic 45.0%
! GTGAAATAGCGGTGTTGAAG+AGG + chr2.4:13229731-13229750 None:intergenic 45.0%
!! AATCGACCCAATTCGGTGTT+CGG - chr2.4:13230189-13230208 MS.gene35522:CDS 45.0%
!! GAGGATTCTGATGGTGTTAC+TGG - chr2.4:13230473-13230492 MS.gene35522:CDS 45.0%
!!! CGGAGAGTTTTTTGTTTGCC+AGG - chr2.4:13230609-13230628 MS.gene35522:CDS 45.0%
ATCTCCATTTCACCCCTCTC+TGG - chr2.4:13229853-13229872 MS.gene35522:CDS 50.0%
ATCTGTTGGGGAGATTGGGA+TGG + chr2.4:13229691-13229710 None:intergenic 50.0%
CCATCCTCATACGTATCAGC+CGG - chr2.4:13230538-13230557 MS.gene35522:CDS 50.0%
CTCAACCGGAGAATAAGCTC+CGG + chr2.4:13230575-13230594 None:intergenic 50.0%
GAGAGGGGTGAAATGGAGAT+CGG + chr2.4:13229853-13229872 None:intergenic 50.0%
GATTCCAGAGAGGGGTGAAA+TGG + chr2.4:13229860-13229879 None:intergenic 50.0%
TCTGTTGGGGAGATTGGGAT+GGG + chr2.4:13229690-13229709 None:intergenic 50.0%
TGTCACCGGAGCTTATTCTC+CGG - chr2.4:13230567-13230586 MS.gene35522:CDS 50.0%
! AGGGGTGTGAAGAGGAAGAA+GGG + chr2.4:13229764-13229783 None:intergenic 50.0%
! GAATTTGGAGGGGTGTGAAG+AGG + chr2.4:13229772-13229791 None:intergenic 50.0%
!! AAGCATTGGAAGCATCGTCG+AGG + chr2.4:13230070-13230089 None:intergenic 50.0%
AGAAGCAAGTAAACGGCGCG+TGG + chr2.4:13230042-13230061 None:intergenic 55.0%
ATTGGGATGGGGGTAGGATG+AGG + chr2.4:13229678-13229697 None:intergenic 55.0%
CCGGCTGATACGTATGAGGA+TGG + chr2.4:13230541-13230560 None:intergenic 55.0%
CTGTTGGGGAGATTGGGATG+GGG + chr2.4:13229689-13229708 None:intergenic 55.0%
GGATGAGGTGGGAGAATAGC+AGG + chr2.4:13229663-13229682 None:intergenic 55.0%
GGGGTGTGAAGAGGAAGAAG+GGG + chr2.4:13229763-13229782 None:intergenic 55.0%
GGGTGTGAAGAGGAAGAAGG+GGG + chr2.4:13229762-13229781 None:intergenic 55.0%
TCCTCATACGTATCAGCCGG+AGG - chr2.4:13230541-13230560 MS.gene35522:CDS 55.0%
TCCTCCGGCTGATACGTATG+AGG + chr2.4:13230545-13230564 None:intergenic 55.0%
TGGACGTTACGCGATGATGC+CGG - chr2.4:13230280-13230299 MS.gene35522:CDS 55.0%
TGTTGGGGAGATTGGGATGG+GGG + chr2.4:13229688-13229707 None:intergenic 55.0%
! CGCGTGGCGGAGATTAAAGT+GGG + chr2.4:13230026-13230045 None:intergenic 55.0%
! CGTGGCGGAGATTAAAGTGG+GGG + chr2.4:13230024-13230043 None:intergenic 55.0%
! GAGGGGTGTGAAGAGGAAGA+AGG + chr2.4:13229765-13229784 None:intergenic 55.0%
! GCGTGGCGGAGATTAAAGTG+GGG + chr2.4:13230025-13230044 None:intergenic 55.0%
! TAAAGTGGGGGAAGCACGGT+AGG + chr2.4:13230012-13230031 None:intergenic 55.0%
AGCAAGTAAACGGCGCGTGG+CGG + chr2.4:13230039-13230058 None:intergenic 60.0%
AGCTCCGGTGACACTTCCTC+CGG + chr2.4:13230560-13230579 None:intergenic 60.0%
GGATGGGGGTAGGATGAGGT+GGG + chr2.4:13229674-13229693 None:intergenic 60.0%
! GCGCGTGGCGGAGATTAAAG+TGG + chr2.4:13230027-13230046 None:intergenic 60.0%
! TGCTTAAACGGAGGCGCGTG+AGG + chr2.4:13229947-13229966 None:intergenic 60.0%
!! TCAGCCGGAGGAAGTGTCAC+CGG - chr2.4:13230553-13230572 MS.gene35522:CDS 60.0%
AGGCGCGTGAGGTTAACGCG+CGG + chr2.4:13229936-13229955 None:intergenic 65.0%
CGTGAGGTTAACGCGCGGGT+CGG + chr2.4:13229931-13229950 None:intergenic 65.0%
GGGATGGGGGTAGGATGAGG+TGG + chr2.4:13229675-13229694 None:intergenic 65.0%
GGGGAGATTGGGATGGGGGT+AGG + chr2.4:13229684-13229703 None:intergenic 65.0%
GGTTAACGCGCGGGTCGGAA+TGG + chr2.4:13229926-13229945 None:intergenic 65.0%
!! TGGAAGCGTTACACGGCGCG+TGG - chr2.4:13230260-13230279 MS.gene35522:CDS 65.0%
GGCGCGTGAGGTTAACGCGC+GGG + chr2.4:13229935-13229954 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 13229591 13230697 13229591 ID=MS.gene35522
chr2.4 mRNA 13229591 13230697 13229591 ID=MS.gene35522.t1;Parent=MS.gene35522
chr2.4 exon 13229591 13230697 13229591 ID=MS.gene35522.t1.exon1;Parent=MS.gene35522.t1
chr2.4 CDS 13229591 13230697 13229591 ID=cds.MS.gene35522.t1;Parent=MS.gene35522.t1
Gene Sequence

>MS.gene35522

ATGTTCTCAAACCCATATCTTCTAACTTTCTCTCTCCTTCTCTCTCTTCCAATTCTCTTCTTTCTCGCTCCTGCTATTCTCCCACCTCATCCTACCCCCATCCCAATCTCCCCAACAGATGAACTCGATGACATCAACCTCTTCAACACCGCTATTTCACATTCTACACCCCCTTCTTCCTCTTCACACCCCTCCAAATTCTTTCATCTCTCTTCCAAAAACCCTACCTTCAAAATTGCTTTCCTTTTCCTCACAAACACCGATCTCCATTTCACCCCTCTCTGGAATCTCTTCTTTCAAACAACCCCATCTAAATTATTCAATGTTTATGTCCATTCCGACCCGCGCGTTAACCTCACGCGCCTCCGTTTAAGCAACAGTTACAACCCCATCTTCAAATTTGTACCGTCTAAGAAAACCTACCGTGCTTCCCCCACTTTAATCTCCGCCACGCGCCGTTTACTTGCTTCTGCGATCCTCGACGATGCTTCCAATGCTTATTTCATCGTTCTTTCACAATATTGTATCCCTCTTCATTCGTTTGATTATATCTATAAATCACTTTTTCTTTCACCTACGTTTGATTTAACCGATTCGGAATCGACCCAATTCGGTGTTCGGTTGAAATATAAAAGCTTTATTGAAATTATCAACAATGGACCTAGGTTATGGAAGCGTTACACGGCGCGTGGACGTTACGCGATGATGCCGGAAGTTCCGTTTGAGAAATTTCGTGTTGGGTCGCAGTTTTTTACTTTGACAAGAAAACATGCTTTGGTTGTTGTTAAGGATAGGACATTGTGGAGGAAGTTTAAGGTTCCTTGTTACCGTGATGATGAGTGTTATCCTGAGGAACATTATTTTCCTACGTTGTTGTCTATGGAGGATTCTGATGGTGTTACTGGCTATACACTTACCAATGTGAATTGGACTGGGACTGTTAATGGCCATCCTCATACGTATCAGCCGGAGGAAGTGTCACCGGAGCTTATTCTCCGGTTGAGAAAGTCGAATAATTCGGAGAGTTTTTTGTTTGCCAGGAAATTTGTTCCTGATTGCTTGGAACCATTGATGGGTATTGCCAAATCTGTTATTTTCAAAGATTGA
Protein sequence

>MS.gene35522.t1

MFSNPYLLTFSLLLSLPILFFLAPAILPPHPTPIPISPTDELDDINLFNTAISHSTPPSSSSHPSKFFHLSSKNPTFKIAFLFLTNTDLHFTPLWNLFFQTTPSKLFNVYVHSDPRVNLTRLRLSNSYNPIFKFVPSKKTYRASPTLISATRRLLASAILDDASNAYFIVLSQYCIPLHSFDYIYKSLFLSPTFDLTDSESTQFGVRLKYKSFIEIINNGPRLWKRYTARGRYAMMPEVPFEKFRVGSQFFTLTRKHALVVVKDRTLWRKFKVPCYRDDECYPEEHYFPTLLSMEDSDGVTGYTLTNVNWTGTVNGHPHTYQPEEVSPELILRLRKSNNSESFLFARKFVPDCLEPLMGIAKSVIFKD