AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43341

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43341.t1 MTR_1g048320 91.279 344 25 2 1 339 1 344 0.0 612
MS.gene43341.t1 MTR_1g031060 71.963 321 82 5 24 339 45 362 3.60e-149 424
MS.gene43341.t1 MTR_1g031080 76.518 247 53 3 97 339 47 292 7.45e-134 383
MS.gene43341.t1 MTR_2g087610 56.081 296 118 4 56 339 73 368 3.25e-107 318
MS.gene43341.t1 MTR_3g106930 48.475 295 124 6 61 339 95 377 1.73e-80 250
MS.gene43341.t1 MTR_1g110290 35.806 310 174 6 35 336 99 391 3.25e-50 172
MS.gene43341.t1 MTR_2g005350 39.024 287 147 9 60 336 168 436 8.53e-50 172
MS.gene43341.t1 MTR_4g127550 34.211 304 147 6 59 331 106 387 6.95e-49 169
MS.gene43341.t1 MTR_2g037600 33.115 305 152 6 58 331 103 386 7.65e-48 166
MS.gene43341.t1 MTR_5g055480 38.328 287 147 8 60 336 122 388 1.11e-47 165
MS.gene43341.t1 MTR_8g015500 35.315 286 159 7 60 336 143 411 6.39e-47 164
MS.gene43341.t1 MTR_1g009960 35.082 305 152 8 53 329 104 390 3.21e-46 162
MS.gene43341.t1 MTR_1g009960 36.466 266 146 7 53 313 104 351 2.51e-45 159
MS.gene43341.t1 MTR_2g037620 33.003 303 150 6 60 331 97 377 3.28e-45 159
MS.gene43341.t1 MTR_3g108740 36.246 309 139 10 55 329 87 371 3.88e-45 158
MS.gene43341.t1 MTR_1g016880 34.164 281 158 7 60 331 106 368 9.64e-45 157
MS.gene43341.t1 MTR_5g006930 36.620 284 150 9 60 332 116 380 4.87e-44 155
MS.gene43341.t1 MTR_6g083830 35.336 283 155 7 60 332 149 413 3.25e-43 154
MS.gene43341.t1 MTR_5g006920 35.915 284 152 9 60 332 151 415 4.41e-42 151
MS.gene43341.t1 MTR_3g104840 34.202 307 158 7 35 332 116 387 2.27e-41 149
MS.gene43341.t1 MTR_2g024120 29.464 336 164 13 39 321 36 351 1.87e-28 114
MS.gene43341.t1 MTR_8g094180 27.829 327 165 9 59 332 54 362 3.88e-26 107
MS.gene43341.t1 MTR_8g094180 29.818 275 145 8 59 303 54 310 6.03e-26 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43341.t1 AT3G52060 57.962 314 113 6 35 339 43 346 5.20e-124 360
MS.gene43341.t1 AT5G22070 50.975 359 145 9 9 339 7 362 3.80e-115 338
MS.gene43341.t1 AT5G25330 44.343 327 143 10 33 339 55 362 1.84e-78 244
MS.gene43341.t1 AT4G32290 45.820 323 141 7 37 339 71 379 9.00e-77 241
MS.gene43341.t1 AT4G31350 38.206 301 136 7 56 329 84 361 4.55e-59 194
MS.gene43341.t1 AT4G31350 38.742 302 138 8 56 329 84 366 6.42e-58 192
MS.gene43341.t1 AT4G31350 38.742 302 138 8 56 329 84 366 6.42e-58 192
MS.gene43341.t1 AT4G31350 38.742 302 138 8 56 329 84 366 6.42e-58 192
MS.gene43341.t1 AT5G25970 37.895 285 150 8 57 332 115 381 1.28e-50 173
MS.gene43341.t1 AT5G25970 37.895 285 150 8 57 332 115 381 1.28e-50 173
MS.gene43341.t1 AT5G25970 37.895 285 150 8 57 332 115 381 1.28e-50 173
MS.gene43341.t1 AT3G21310 36.713 286 154 7 60 336 114 381 6.91e-50 171
MS.gene43341.t1 AT3G21310 36.713 286 154 7 60 336 114 381 6.91e-50 171
MS.gene43341.t1 AT1G51770 35.664 286 157 7 60 336 123 390 5.26e-48 167
MS.gene43341.t1 AT5G11730 34.965 286 159 7 60 336 117 384 1.06e-47 165
MS.gene43341.t1 AT1G10280 34.843 287 159 7 60 336 142 410 1.31e-45 160
MS.gene43341.t1 AT1G10880 34.520 281 159 6 60 332 117 380 1.76e-45 159
MS.gene43341.t1 AT1G68390 33.754 317 173 8 35 336 112 406 3.23e-44 157
MS.gene43341.t1 AT2G19160 33.993 303 146 7 61 331 106 386 9.27e-44 155
MS.gene43341.t1 AT2G19160 33.993 303 146 7 61 331 106 386 9.27e-44 155
MS.gene43341.t1 AT1G68380 34.877 324 174 9 28 337 91 391 1.40e-43 154
MS.gene43341.t1 AT4G25870 34.731 334 155 12 33 331 75 380 1.89e-43 154
MS.gene43341.t1 AT4G30060 33.993 303 146 7 61 331 109 389 1.10e-42 152
MS.gene43341.t1 AT4G30060 33.993 303 146 7 61 331 109 389 1.10e-42 152
MS.gene43341.t1 AT5G57270 35.252 278 149 6 59 327 96 351 7.66e-42 150
MS.gene43341.t1 AT5G57270 35.252 278 149 6 59 327 96 351 7.66e-42 150
MS.gene43341.t1 AT5G57270 35.252 278 149 6 59 327 96 351 7.66e-42 150
MS.gene43341.t1 AT5G57270 35.252 278 149 6 59 327 96 351 7.66e-42 150
MS.gene43341.t1 AT5G57270 35.252 278 149 6 59 327 96 351 7.66e-42 150
MS.gene43341.t1 AT5G16170 33.333 285 160 6 61 332 136 403 4.46e-40 145
MS.gene43341.t1 AT1G73810 36.644 292 147 11 61 336 147 416 4.93e-39 143
MS.gene43341.t1 AT1G51770 31.469 286 142 7 60 336 123 363 3.93e-35 132
MS.gene43341.t1 AT5G14550 30.743 296 166 9 44 318 44 321 7.87e-32 122
MS.gene43341.t1 AT5G14550 29.155 343 172 11 44 333 44 368 4.11e-30 118
MS.gene43341.t1 AT5G14550 29.154 319 173 10 44 327 44 344 5.72e-30 118
MS.gene43341.t1 AT1G11940 29.052 327 166 10 45 324 58 365 1.21e-28 114
MS.gene43341.t1 AT1G62305 28.418 373 182 14 3 321 15 356 1.31e-26 108
MS.gene43341.t1 AT1G62305 30.717 293 144 12 3 267 15 276 2.31e-23 99.0
MS.gene43341.t1 AT5G14550 28.571 329 165 13 44 333 44 341 9.13e-23 97.8
MS.gene43341.t1 AT1G11940 33.173 208 115 7 45 247 58 246 4.11e-20 89.0
MS.gene43341.t1 AT3G21310 45.133 113 59 3 60 171 114 224 1.21e-19 87.4
MS.gene43341.t1 AT1G62305 26.810 373 164 14 3 321 15 332 1.21e-18 86.3

Find 108 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCACCTTTGCTCCTTTA+TGG 0.074069 1.4:+33414402 MS.gene43341:CDS
TTGAATCTTGTACTTGTTTA+AGG 0.108034 1.4:-33414711 None:intergenic
TAGGAAGAGGAAGGCGATTT+TGG 0.185523 1.4:-33414369 None:intergenic
GTTGAAGAGGTGGTTGTTTC+CGG 0.217030 1.4:-33414438 None:intergenic
GTTGGAAAGTAATGCTCTTC+TGG 0.233444 1.4:-33414953 None:intergenic
CCTCACCTTTGCTCCTTTAT+GGG 0.265226 1.4:+33414403 MS.gene43341:CDS
CCTTTGCATGGTTCCGGTTC+AGG 0.283118 1.4:-33414862 None:intergenic
AGGTCCTCCGCGTCGGGGTC+AGG 0.290705 1.4:-33414284 None:intergenic
AAGGGATGAAGTGGCTTTGA+TGG 0.297513 1.4:-33414514 None:intergenic
AAAAGTTGTGGAAGAAGTTT+CGG 0.336219 1.4:+33414900 MS.gene43341:CDS
TTACTCTTACGAGAGTTAAC+TGG 0.341127 1.4:+33415014 MS.gene43341:CDS
AGGTGAGGTTGGTATTTGTT+AGG 0.341432 1.4:-33414388 None:intergenic
GAACAACCTTTGCATGGTTC+CGG 0.347319 1.4:-33414868 None:intergenic
GTAATGCTCTTCTGGGTAAC+AGG 0.352431 1.4:-33414945 None:intergenic
TTATGGGAAATATTCTTTGC+CGG 0.354403 1.4:+33414419 MS.gene43341:CDS
CGCAGTTCTTTATCCTGAAC+CGG 0.364355 1.4:+33414849 MS.gene43341:CDS
CGGGGTCAGGGTTGGAAAAC+GGG 0.368387 1.4:-33414271 None:intergenic
TCTCTTAGCCACTGCTATAT+TGG 0.370952 1.4:+33414586 MS.gene43341:CDS
GGGTGAGACAACAGAGGAAA+TGG 0.381450 1.4:-33414480 None:intergenic
ATCAACGGTGCTAGAGTTTC+CGG 0.389809 1.4:-33415154 None:intergenic
AGAGCAAGGTCCTCCGCGTC+GGG 0.395834 1.4:-33414290 None:intergenic
TGTACGCCAGCTTAGGAAAT+CGG 0.400199 1.4:+33415093 MS.gene43341:CDS
GCAAAGAATATTTCCCATAA+AGG 0.406338 1.4:-33414416 None:intergenic
GTTCTTTAGGTTAAATAAGT+GGG 0.421988 1.4:-33414339 None:intergenic
TCGGGGTCAGGGTTGGAAAA+CGG 0.425351 1.4:-33414272 None:intergenic
GGAAACTCTAGCACCGTTGA+TGG 0.426222 1.4:+33415156 MS.gene43341:CDS
CGGAGCTTGTACGCCAGCTT+AGG 0.440581 1.4:+33415086 MS.gene43341:CDS
GGTCCTCCGCGTCGGGGTCA+GGG 0.440712 1.4:-33414283 None:intergenic
GCTTTGATGGAAGACGCCTC+CGG 0.443380 1.4:-33414501 None:intergenic
GGTTGGTATTTGTTAGGAAG+AGG 0.449439 1.4:-33414382 None:intergenic
GTAAAACAAGTGCAGCCATC+CGG 0.449459 1.4:-33414995 None:intergenic
CTCTCTTCCGCCGCGCCACA+AGG 0.462891 1.4:+33414309 MS.gene43341:CDS
TCTTCTGTCGATGGAGGACC+CGG 0.466449 1.4:+33414976 MS.gene43341:CDS
CTCCGCGTCGGGGTCAGGGT+TGG 0.467120 1.4:-33414279 None:intergenic
AATACAATGTTGGGAGAGTA+AGG 0.471648 1.4:-33414633 None:intergenic
TTGGAAAGTAATGCTCTTCT+GGG 0.472293 1.4:-33414952 None:intergenic
GATGCCGGAGGTTTCATTCA+AGG 0.472977 1.4:+33414811 MS.gene43341:CDS
GGTTCTTTAGGTTAAATAAG+TGG 0.477319 1.4:-33414340 None:intergenic
ATTTCCTCTGTTGTCTCACC+CGG 0.480740 1.4:+33414482 MS.gene43341:CDS
AAGTGACGGAGAGGCACGTT+GGG 0.481033 1.4:-33414546 None:intergenic
GAAGAGTGGAATACAATGTT+GGG 0.491853 1.4:-33414642 None:intergenic
CTTTCCAACTCTTCTGTCGA+TGG 0.492844 1.4:+33414967 MS.gene43341:CDS
GAATTTCCTTGCAAACAGAT+AGG 0.494280 1.4:-33415128 None:intergenic
CTAAGCTGGCGTACAAGCTC+CGG 0.504844 1.4:-33415085 None:intergenic
GAGTCAATCTCAATGAAGCT+AGG 0.506518 1.4:-33414737 None:intergenic
CTTTGATGGAAGACGCCTCC+GGG 0.508919 1.4:-33414500 None:intergenic
CAGGAACTCTTATCTATACA+TGG 0.509702 1.4:-33414926 None:intergenic
TAAAATCCGATTTCCTAAGC+TGG 0.511119 1.4:-33415099 None:intergenic
GTTATTCCTATCTGTTTGCA+AGG 0.511432 1.4:+33415122 MS.gene43341:CDS
TTATAGCGCTGGTAGAGATT+CGG 0.513272 1.4:-33414767 None:intergenic
TTCCACGAGCATTATAGCGC+TGG 0.514680 1.4:-33414778 None:intergenic
TACTCGAGAGGAGGTGTCGC+CGG 0.514897 1.4:+33415066 MS.gene43341:CDS
AGAAGAGTGGAATACAATGT+TGG 0.514911 1.4:-33414643 None:intergenic
TCCTCCATCGACAGAAGAGT+TGG 0.522987 1.4:-33414971 None:intergenic
TGCAGTACAAGTTTCAATCT+CGG 0.523348 1.4:-33415201 None:intergenic
AGTTAACTGGACTGGTTGTA+CGG 0.524550 1.4:+33415027 MS.gene43341:CDS
GGGAGAGTAAGGCAAAGTAT+TGG 0.537563 1.4:-33414622 None:intergenic
AAATCCTTGAATGAAACCTC+CGG 0.545006 1.4:-33414815 None:intergenic
GGTGAGACAACAGAGGAAAT+GGG 0.547850 1.4:-33414479 None:intergenic
CTTACGAGAGTTAACTGGAC+TGG 0.548736 1.4:+33415019 MS.gene43341:CDS
GGTATTTGTTAGGAAGAGGA+AGG 0.549687 1.4:-33414378 None:intergenic
TTCTTTAGGTTAAATAAGTG+GGG 0.550325 1.4:-33414338 None:intergenic
TAAAACAAGTGCAGCCATCC+GGG 0.551737 1.4:-33414994 None:intergenic
GGCTAAGAGACGGCGAGCAG+CGG 0.557898 1.4:-33414573 None:intergenic
GCGCTATAATGCTCGTGGAA+AGG 0.565767 1.4:+33414781 MS.gene43341:CDS
AGGCAAAGTATTGGTTATGC+GGG 0.566058 1.4:-33414613 None:intergenic
AGTCAATCTCAATGAAGCTA+GGG 0.567722 1.4:-33414736 None:intergenic
TGGAAAGGATGTAATGATGC+CGG 0.568418 1.4:+33414796 MS.gene43341:CDS
GAAAACGGGAAGTAAGATTG+AGG 0.570111 1.4:-33414257 None:intergenic
TAAAGGAGCAAAGGTGAGGT+TGG 0.572056 1.4:-33414399 None:intergenic
TTCTGCGAGATCAAAAGTTG+TGG 0.575067 1.4:+33414888 MS.gene43341:CDS
GAGAGCAAGGTCCTCCGCGT+CGG 0.575874 1.4:-33414291 None:intergenic
AAAACGGGAAGTAAGATTGA+GGG 0.576076 1.4:-33414256 None:intergenic
TCCTCTCGAGTATAAAGATG+CGG 0.582936 1.4:-33415055 None:intergenic
TCCTCTGTTGTCTCACCCGG+AGG 0.583294 1.4:+33414485 MS.gene43341:CDS
TACCAGCGCTATAATGCTCG+TGG 0.583876 1.4:+33414776 MS.gene43341:CDS
CTCGCAGAACAACCTTTGCA+TGG 0.585317 1.4:-33414874 None:intergenic
GTTTGCAAGGAAATTCTCAC+CGG 0.585980 1.4:+33415135 MS.gene43341:CDS
TCCAACTCTTCTGTCGATGG+AGG 0.592551 1.4:+33414970 MS.gene43341:CDS
CCCATAAAGGAGCAAAGGTG+AGG 0.594602 1.4:-33414403 None:intergenic
TGGCGCGGCGGAAGAGAGCA+AGG 0.594820 1.4:-33414304 None:intergenic
CTGTCGATGGAGGACCCGGA+TGG 0.595444 1.4:+33414980 MS.gene43341:CDS
CCTCTCGAGTATAAAGATGC+GGG 0.597296 1.4:-33415054 None:intergenic
AAGGCAAAGTATTGGTTATG+CGG 0.608135 1.4:-33414614 None:intergenic
TATTTCCCATAAAGGAGCAA+AGG 0.610784 1.4:-33414408 None:intergenic
ACAAATCGAAACGAGAAGAG+TGG 0.612100 1.4:-33414656 None:intergenic
CGAGCAGCGGCTATAAGTGA+CGG 0.620401 1.4:-33414560 None:intergenic
AAACGGGAAGTAAGATTGAG+GGG 0.627961 1.4:-33414255 None:intergenic
ATATAGCAGTGGCTAAGAGA+CGG 0.629423 1.4:-33414583 None:intergenic
CAACCCTGACCCCGACGCGG+AGG 0.630344 1.4:+33414280 MS.gene43341:CDS
GGATGTAGATGTTGAAGAGG+TGG 0.641305 1.4:-33414448 None:intergenic
TTCCAACCCTGACCCCGACG+CGG 0.643455 1.4:+33414277 MS.gene43341:CDS
TCATAAGCTATATCCATCAA+CGG 0.653143 1.4:-33415169 None:intergenic
CGGGTCATCCAATATAGCAG+TGG 0.653653 1.4:-33414594 None:intergenic
CGTGGATGTAGATGTTGAAG+AGG 0.655044 1.4:-33414451 None:intergenic
GGGGTGTTGTCCTTGTGGCG+CGG 0.655134 1.4:-33414319 None:intergenic
GAGCAAGGTCCTCCGCGTCG+GGG 0.655727 1.4:-33414289 None:intergenic
TAAGTGACGGAGAGGCACGT+TGG 0.660309 1.4:-33414547 None:intergenic
CCTGAACCGGAACCATGCAA+AGG 0.664001 1.4:+33414862 MS.gene43341:CDS
AGCGGCTATAAGTGACGGAG+AGG 0.667766 1.4:-33414555 None:intergenic
CCCGCATCTTTATACTCGAG+AGG 0.669713 1.4:+33415054 MS.gene43341:CDS
CTCGAGTATAAAGATGCGGG+TGG 0.683495 1.4:-33415051 None:intergenic
TAAGTGGGGTGTTGTCCTTG+TGG 0.684395 1.4:-33414324 None:intergenic
GCATCTTTATACTCGAGAGG+AGG 0.684710 1.4:+33415057 MS.gene43341:CDS
GTGTTGTCCTTGTGGCGCGG+CGG 0.702688 1.4:-33414316 None:intergenic
CAGAGGAAATGGGATCTGCG+TGG 0.703706 1.4:-33414469 None:intergenic
AAAGGATGTAATGATGCCGG+AGG 0.717780 1.4:+33414799 MS.gene43341:CDS
GCCTCCGGGTGAGACAACAG+AGG 0.763777 1.4:-33414486 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGAATCTTGTACTTGTTTA+AGG - chr1.4:33414714-33414733 None:intergenic 25.0%
!! GTTCTTTAGGTTAAATAAGT+GGG - chr1.4:33414342-33414361 None:intergenic 25.0%
!! TTCTTTAGGTTAAATAAGTG+GGG - chr1.4:33414341-33414360 None:intergenic 25.0%
!!! ATAACTCCCTTTTTGAAAAT+CGG + chr1.4:33414681-33414700 MS.gene43341:CDS 25.0%
AAAAGTTGTGGAAGAAGTTT+CGG + chr1.4:33414900-33414919 MS.gene43341:CDS 30.0%
GCAAAGAATATTTCCCATAA+AGG - chr1.4:33414419-33414438 None:intergenic 30.0%
TCATAAGCTATATCCATCAA+CGG - chr1.4:33415172-33415191 None:intergenic 30.0%
TTATGGGAAATATTCTTTGC+CGG + chr1.4:33414419-33414438 MS.gene43341:CDS 30.0%
! GGTTCTTTAGGTTAAATAAG+TGG - chr1.4:33414343-33414362 None:intergenic 30.0%
!! TAACTCCCTTTTTGAAAATC+GGG + chr1.4:33414682-33414701 MS.gene43341:CDS 30.0%
AAAACGGGAAGTAAGATTGA+GGG - chr1.4:33414259-33414278 None:intergenic 35.0%
AAATCCTTGAATGAAACCTC+CGG - chr1.4:33414818-33414837 None:intergenic 35.0%
AATACAATGTTGGGAGAGTA+AGG - chr1.4:33414636-33414655 None:intergenic 35.0%
AGAAGAGTGGAATACAATGT+TGG - chr1.4:33414646-33414665 None:intergenic 35.0%
AGTCAATCTCAATGAAGCTA+GGG - chr1.4:33414739-33414758 None:intergenic 35.0%
CAGGAACTCTTATCTATACA+TGG - chr1.4:33414929-33414948 None:intergenic 35.0%
GAAGAGTGGAATACAATGTT+GGG - chr1.4:33414645-33414664 None:intergenic 35.0%
GAATTTCCTTGCAAACAGAT+AGG - chr1.4:33415131-33415150 None:intergenic 35.0%
GTTATTCCTATCTGTTTGCA+AGG + chr1.4:33415122-33415141 MS.gene43341:CDS 35.0%
TAAAATCCGATTTCCTAAGC+TGG - chr1.4:33415102-33415121 None:intergenic 35.0%
TATTTCCCATAAAGGAGCAA+AGG - chr1.4:33414411-33414430 None:intergenic 35.0%
TTACTCTTACGAGAGTTAAC+TGG + chr1.4:33415014-33415033 MS.gene43341:CDS 35.0%
TTGGAAAGTAATGCTCTTCT+GGG - chr1.4:33414955-33414974 None:intergenic 35.0%
! ACTCTACCCGATTTTCAAAA+AGG - chr1.4:33414691-33414710 None:intergenic 35.0%
! CTCTACCCGATTTTCAAAAA+GGG - chr1.4:33414690-33414709 None:intergenic 35.0%
! TCATTCAAGGATTTTCGCAA+AGG + chr1.4:33414824-33414843 MS.gene43341:CDS 35.0%
!! AAGGCAAAGTATTGGTTATG+CGG - chr1.4:33414617-33414636 None:intergenic 35.0%
!!! TTGTTTTAGAAGGGATGAAG+TGG - chr1.4:33414526-33414545 None:intergenic 35.0%
!!! TTTTGGGTTTAGGGTTCTTT+AGG - chr1.4:33414355-33414374 None:intergenic 35.0%
AAACGGGAAGTAAGATTGAG+GGG - chr1.4:33414258-33414277 None:intergenic 40.0%
ACAAATCGAAACGAGAAGAG+TGG - chr1.4:33414659-33414678 None:intergenic 40.0%
ATATAGCAGTGGCTAAGAGA+CGG - chr1.4:33414586-33414605 None:intergenic 40.0%
GAAAACGGGAAGTAAGATTG+AGG - chr1.4:33414260-33414279 None:intergenic 40.0%
GAGTCAATCTCAATGAAGCT+AGG - chr1.4:33414740-33414759 None:intergenic 40.0%
GTTGGAAAGTAATGCTCTTC+TGG - chr1.4:33414956-33414975 None:intergenic 40.0%
GTTTGCAAGGAAATTCTCAC+CGG + chr1.4:33415135-33415154 MS.gene43341:CDS 40.0%
TCCTCTCGAGTATAAAGATG+CGG - chr1.4:33415058-33415077 None:intergenic 40.0%
TCTCTTAGCCACTGCTATAT+TGG + chr1.4:33414586-33414605 MS.gene43341:CDS 40.0%
TGGAAAGGATGTAATGATGC+CGG + chr1.4:33414796-33414815 MS.gene43341:CDS 40.0%
TTCTGCGAGATCAAAAGTTG+TGG + chr1.4:33414888-33414907 MS.gene43341:CDS 40.0%
! GGTATTTGTTAGGAAGAGGA+AGG - chr1.4:33414381-33414400 None:intergenic 40.0%
! GGTTGGTATTTGTTAGGAAG+AGG - chr1.4:33414385-33414404 None:intergenic 40.0%
!! AGGCAAAGTATTGGTTATGC+GGG - chr1.4:33414616-33414635 None:intergenic 40.0%
!! AGGTGAGGTTGGTATTTGTT+AGG - chr1.4:33414391-33414410 None:intergenic 40.0%
!! AGTTAACTGGACTGGTTGTA+CGG + chr1.4:33415027-33415046 MS.gene43341:CDS 40.0%
!! TTATAGCGCTGGTAGAGATT+CGG - chr1.4:33414770-33414789 None:intergenic 40.0%
!!! CACGTTGGGTTGTTTTAGAA+GGG - chr1.4:33414535-33414554 None:intergenic 40.0%
AAAGGATGTAATGATGCCGG+AGG + chr1.4:33414799-33414818 MS.gene43341:CDS 45.0%
ACCTCACCTTTGCTCCTTTA+TGG + chr1.4:33414402-33414421 MS.gene43341:CDS 45.0%
ATTTCCTCTGTTGTCTCACC+CGG + chr1.4:33414482-33414501 MS.gene43341:CDS 45.0%
CCTCACCTTTGCTCCTTTAT+GGG + chr1.4:33414403-33414422 MS.gene43341:CDS 45.0%
CCTCTCGAGTATAAAGATGC+GGG - chr1.4:33415057-33415076 None:intergenic 45.0%
CGCAGTTCTTTATCCTGAAC+CGG + chr1.4:33414849-33414868 MS.gene43341:CDS 45.0%
CGTGGATGTAGATGTTGAAG+AGG - chr1.4:33414454-33414473 None:intergenic 45.0%
CTTACGAGAGTTAACTGGAC+TGG + chr1.4:33415019-33415038 MS.gene43341:CDS 45.0%
CTTTCCAACTCTTCTGTCGA+TGG + chr1.4:33414967-33414986 MS.gene43341:CDS 45.0%
GAACAACCTTTGCATGGTTC+CGG - chr1.4:33414871-33414890 None:intergenic 45.0%
GCATCTTTATACTCGAGAGG+AGG + chr1.4:33415057-33415076 MS.gene43341:CDS 45.0%
GGATGTAGATGTTGAAGAGG+TGG - chr1.4:33414451-33414470 None:intergenic 45.0%
GGGAGAGTAAGGCAAAGTAT+TGG - chr1.4:33414625-33414644 None:intergenic 45.0%
GGTGAGACAACAGAGGAAAT+GGG - chr1.4:33414482-33414501 None:intergenic 45.0%
GTAAAACAAGTGCAGCCATC+CGG - chr1.4:33414998-33415017 None:intergenic 45.0%
GTAATGCTCTTCTGGGTAAC+AGG - chr1.4:33414948-33414967 None:intergenic 45.0%
TAAAACAAGTGCAGCCATCC+GGG - chr1.4:33414997-33415016 None:intergenic 45.0%
TAAAGGAGCAAAGGTGAGGT+TGG - chr1.4:33414402-33414421 None:intergenic 45.0%
TGTACGCCAGCTTAGGAAAT+CGG + chr1.4:33415093-33415112 MS.gene43341:CDS 45.0%
! AGGAAGAGGAAGGCGATTTT+GGG - chr1.4:33414371-33414390 None:intergenic 45.0%
! ATCAACGGTGCTAGAGTTTC+CGG - chr1.4:33415157-33415176 None:intergenic 45.0%
! GTTGAAGAGGTGGTTGTTTC+CGG - chr1.4:33414441-33414460 None:intergenic 45.0%
! TAGGAAGAGGAAGGCGATTT+TGG - chr1.4:33414372-33414391 None:intergenic 45.0%
!! AAGGGATGAAGTGGCTTTGA+TGG - chr1.4:33414517-33414536 None:intergenic 45.0%
!!! AGGAAGGCGATTTTGGGTTT+AGG - chr1.4:33414365-33414384 None:intergenic 45.0%
!!! GCACGTTGGGTTGTTTTAGA+AGG - chr1.4:33414536-33414555 None:intergenic 45.0%
!!! GGAAGGCGATTTTGGGTTTA+GGG - chr1.4:33414364-33414383 None:intergenic 45.0%
CCCATAAAGGAGCAAAGGTG+AGG - chr1.4:33414406-33414425 None:intergenic 50.0%
CCCGCATCTTTATACTCGAG+AGG + chr1.4:33415054-33415073 MS.gene43341:CDS 50.0%
CGGGTCATCCAATATAGCAG+TGG - chr1.4:33414597-33414616 None:intergenic 50.0%
CTCGAGTATAAAGATGCGGG+TGG - chr1.4:33415054-33415073 None:intergenic 50.0%
CTCGCAGAACAACCTTTGCA+TGG - chr1.4:33414877-33414896 None:intergenic 50.0%
GATGCCGGAGGTTTCATTCA+AGG + chr1.4:33414811-33414830 MS.gene43341:CDS 50.0%
GCGCTATAATGCTCGTGGAA+AGG + chr1.4:33414781-33414800 MS.gene43341:CDS 50.0%
GGAAACTCTAGCACCGTTGA+TGG + chr1.4:33415156-33415175 MS.gene43341:CDS 50.0%
GGGTGAGACAACAGAGGAAA+TGG - chr1.4:33414483-33414502 None:intergenic 50.0%
TACCAGCGCTATAATGCTCG+TGG + chr1.4:33414776-33414795 MS.gene43341:CDS 50.0%
TCCAACTCTTCTGTCGATGG+AGG + chr1.4:33414970-33414989 MS.gene43341:CDS 50.0%
TCCTCCATCGACAGAAGAGT+TGG - chr1.4:33414974-33414993 None:intergenic 50.0%
TTCCACGAGCATTATAGCGC+TGG - chr1.4:33414781-33414800 None:intergenic 50.0%
!! TAAGTGGGGTGTTGTCCTTG+TGG - chr1.4:33414327-33414346 None:intergenic 50.0%
CAGAGGAAATGGGATCTGCG+TGG - chr1.4:33414472-33414491 None:intergenic 55.0%
CCTGAACCGGAACCATGCAA+AGG + chr1.4:33414862-33414881 MS.gene43341:CDS 55.0%
CCTTTGCATGGTTCCGGTTC+AGG - chr1.4:33414865-33414884 None:intergenic 55.0%
CGAGCAGCGGCTATAAGTGA+CGG - chr1.4:33414563-33414582 None:intergenic 55.0%
CTAAGCTGGCGTACAAGCTC+CGG - chr1.4:33415088-33415107 None:intergenic 55.0%
CTTTGATGGAAGACGCCTCC+GGG - chr1.4:33414503-33414522 None:intergenic 55.0%
GCTTTGATGGAAGACGCCTC+CGG - chr1.4:33414504-33414523 None:intergenic 55.0%
TCGGGGTCAGGGTTGGAAAA+CGG - chr1.4:33414275-33414294 None:intergenic 55.0%
TCTTCTGTCGATGGAGGACC+CGG + chr1.4:33414976-33414995 MS.gene43341:CDS 55.0%
! AAGTGACGGAGAGGCACGTT+GGG - chr1.4:33414549-33414568 None:intergenic 55.0%
! AGCGGCTATAAGTGACGGAG+AGG - chr1.4:33414558-33414577 None:intergenic 55.0%
! TAAGTGACGGAGAGGCACGT+TGG - chr1.4:33414550-33414569 None:intergenic 55.0%
CGGAGCTTGTACGCCAGCTT+AGG + chr1.4:33415086-33415105 MS.gene43341:CDS 60.0%
CGGGGTCAGGGTTGGAAAAC+GGG - chr1.4:33414274-33414293 None:intergenic 60.0%
TCCTCTGTTGTCTCACCCGG+AGG + chr1.4:33414485-33414504 MS.gene43341:CDS 60.0%
!! TACTCGAGAGGAGGTGTCGC+CGG + chr1.4:33415066-33415085 MS.gene43341:CDS 60.0%
AGAGCAAGGTCCTCCGCGTC+GGG - chr1.4:33414293-33414312 None:intergenic 65.0%
CTGTCGATGGAGGACCCGGA+TGG + chr1.4:33414980-33414999 MS.gene43341:CDS 65.0%
GAGAGCAAGGTCCTCCGCGT+CGG - chr1.4:33414294-33414313 None:intergenic 65.0%
GCCTCCGGGTGAGACAACAG+AGG - chr1.4:33414489-33414508 None:intergenic 65.0%
GGCTAAGAGACGGCGAGCAG+CGG - chr1.4:33414576-33414595 None:intergenic 65.0%
GTGTTGTCCTTGTGGCGCGG+CGG - chr1.4:33414319-33414338 None:intergenic 65.0%
TTCCAACCCTGACCCCGACG+CGG + chr1.4:33414277-33414296 MS.gene43341:CDS 65.0%
!! GGGGTGTTGTCCTTGTGGCG+CGG - chr1.4:33414322-33414341 None:intergenic 65.0%
CTCTCTTCCGCCGCGCCACA+AGG + chr1.4:33414309-33414328 MS.gene43341:CDS 70.0%
GAGCAAGGTCCTCCGCGTCG+GGG - chr1.4:33414292-33414311 None:intergenic 70.0%
TGGCGCGGCGGAAGAGAGCA+AGG - chr1.4:33414307-33414326 None:intergenic 70.0%
AGGTCCTCCGCGTCGGGGTC+AGG - chr1.4:33414287-33414306 None:intergenic 75.0%
CAACCCTGACCCCGACGCGG+AGG + chr1.4:33414280-33414299 MS.gene43341:CDS 75.0%
CTCCGCGTCGGGGTCAGGGT+TGG - chr1.4:33414282-33414301 None:intergenic 75.0%
GGTCCTCCGCGTCGGGGTCA+GGG - chr1.4:33414286-33414305 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 33414191 33415210 33414191 ID=MS.gene43341
chr1.4 mRNA 33414191 33415210 33414191 ID=MS.gene43341.t1;Parent=MS.gene43341
chr1.4 exon 33414191 33415210 33414191 ID=MS.gene43341.t1.exon1;Parent=MS.gene43341.t1
chr1.4 CDS 33414191 33415210 33414191 ID=cds.MS.gene43341.t1;Parent=MS.gene43341.t1
Gene Sequence

>MS.gene43341

ATGTCAAAATTACGCTTTCTGATCGTCTTGTTATTTGCTCTCTTAATCGCTCTCTTCTTTTTCTCCCCTCAATCTTACTTCCCGTTTTCCAACCCTGACCCCGACGCGGAGGACCTTGCTCTCTTCCGCCGCGCCACAAGGACAACACCCCACTTATTTAACCTAAAGAACCCTAAACCCAAAATCGCCTTCCTCTTCCTAACAAATACCAACCTCACCTTTGCTCCTTTATGGGAAATATTCTTTGCCGGAAACAACCACCTCTTCAACATCTACATCCACGCAGATCCCATTTCCTCTGTTGTCTCACCCGGAGGCGTCTTCCATCAAAGCCACTTCATCCCTTCTAAAACAACCCAACGTGCCTCTCCGTCACTTATAGCCGCTGCTCGCCGTCTCTTAGCCACTGCTATATTGGATGACCCGCATAACCAATACTTTGCCTTACTCTCCCAACATTGTATTCCACTCTTCTCGTTTCGATTTGTTTATAACTCCCTTTTTGAAAATCGGGTAGAGTCCTTAAACAAGTACAAGATTCAACACCCTAGCTTCATTGAGATTGACTCTAAACATCCGAATCTCTACCAGCGCTATAATGCTCGTGGAAAGGATGTAATGATGCCGGAGGTTTCATTCAAGGATTTTCGCAAAGGCTCGCAGTTCTTTATCCTGAACCGGAACCATGCAAAGGTTGTTCTGCGAGATCAAAAGTTGTGGAAGAAGTTTCGGATTCCATGTATAGATAAGAGTTCCTGTTACCCAGAAGAGCATTACTTTCCAACTCTTCTGTCGATGGAGGACCCGGATGGCTGCACTTGTTTTACTCTTACGAGAGTTAACTGGACTGGTTGTACGGACCACCCGCATCTTTATACTCGAGAGGAGGTGTCGCCGGAGCTTGTACGCCAGCTTAGGAAATCGGATTTTAGTTATTCCTATCTGTTTGCAAGGAAATTCTCACCGGAAACTCTAGCACCGTTGATGGATATAGCTTATGATGTCATTTTCCGAGATTGA
Protein sequence

>MS.gene43341.t1

MSKLRFLIVLLFALLIALFFFSPQSYFPFSNPDPDAEDLALFRRATRTTPHLFNLKNPKPKIAFLFLTNTNLTFAPLWEIFFAGNNHLFNIYIHADPISSVVSPGGVFHQSHFIPSKTTQRASPSLIAAARRLLATAILDDPHNQYFALLSQHCIPLFSFRFVYNSLFENRVESLNKYKIQHPSFIEIDSKHPNLYQRYNARGKDVMMPEVSFKDFRKGSQFFILNRNHAKVVLRDQKLWKKFRIPCIDKSSCYPEEHYFPTLLSMEDPDGCTCFTLTRVNWTGCTDHPHLYTREEVSPELVRQLRKSDFSYSYLFARKFSPETLAPLMDIAYDVIFRD