Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44015.t1 | XP_003591011.2 | 99.7 | 303 | 1 | 0 | 1 | 303 | 1 | 303 | 2.40E-171 | 611.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44015.t1 | G7I6W7 | 99.7 | 303 | 1 | 0 | 1 | 303 | 1 | 303 | 1.7e-171 | 611.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene44015 | 0.803626 | 3.19E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene44015 | 0.801261 | 9.79E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene44015 | 0.802416 | 5.67E-49 | -1.69E-46 |
| MS.gene050787 | MS.gene44015 | 0.817469 | 3.25E-52 | -1.69E-46 |
| MS.gene05414 | MS.gene44015 | 0.806694 | 7.26E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene44015 | -0.811688 | 6.18E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene44015 | 0.821097 | 4.84E-53 | -1.69E-46 |
| MS.gene059272 | MS.gene44015 | 0.806121 | 9.59E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene44015 | 0.801527 | 8.63E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene44015 | 0.813374 | 2.64E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44015.t1 | MTR_1g081260 | 99.670 | 303 | 1 | 0 | 1 | 303 | 1 | 303 | 0.0 | 620 |
| MS.gene44015.t1 | MTR_7g114790 | 72.026 | 311 | 72 | 7 | 1 | 302 | 1 | 305 | 1.53e-156 | 439 |
| MS.gene44015.t1 | MTR_7g095100 | 64.725 | 309 | 101 | 4 | 1 | 303 | 1 | 307 | 2.74e-144 | 408 |
| MS.gene44015.t1 | MTR_3g076610 | 35.507 | 276 | 152 | 3 | 4 | 257 | 7 | 278 | 1.94e-61 | 198 |
| MS.gene44015.t1 | MTR_5g069570 | 37.647 | 255 | 155 | 3 | 10 | 260 | 14 | 268 | 2.19e-60 | 195 |
| MS.gene44015.t1 | MTR_4g104760 | 36.122 | 263 | 152 | 6 | 4 | 253 | 7 | 266 | 2.63e-59 | 192 |
| MS.gene44015.t1 | MTR_3g082430 | 40.773 | 233 | 133 | 3 | 26 | 253 | 12 | 244 | 6.50e-58 | 188 |
| MS.gene44015.t1 | MTR_4g055230 | 36.760 | 321 | 166 | 10 | 1 | 301 | 1 | 304 | 1.28e-57 | 187 |
| MS.gene44015.t1 | MTR_2g084965 | 38.433 | 268 | 146 | 7 | 6 | 254 | 8 | 275 | 4.34e-50 | 168 |
| MS.gene44015.t1 | MTR_7g103900 | 33.129 | 326 | 177 | 10 | 1 | 302 | 1 | 309 | 3.23e-49 | 166 |
| MS.gene44015.t1 | MTR_3g106250 | 36.466 | 266 | 142 | 8 | 4 | 253 | 6 | 260 | 3.65e-49 | 167 |
| MS.gene44015.t1 | MTR_5g096570 | 33.910 | 289 | 175 | 5 | 23 | 301 | 1178 | 1460 | 1.11e-48 | 174 |
| MS.gene44015.t1 | MTR_8g069535 | 80.488 | 82 | 15 | 1 | 8 | 89 | 9 | 89 | 1.15e-32 | 121 |
| MS.gene44015.t1 | MTR_8g069535 | 66.102 | 59 | 20 | 0 | 176 | 234 | 89 | 147 | 6.21e-17 | 79.0 |
| MS.gene44015.t1 | MTR_1g013100 | 29.643 | 280 | 154 | 13 | 4 | 261 | 8 | 266 | 1.29e-32 | 123 |
| MS.gene44015.t1 | MTR_7g103900 | 30.043 | 233 | 138 | 7 | 1 | 212 | 1 | 229 | 2.29e-28 | 109 |
| MS.gene44015.t1 | MTR_5g069550 | 32.143 | 168 | 98 | 2 | 10 | 175 | 14 | 167 | 1.55e-24 | 98.2 |
| MS.gene44015.t1 | MTR_0716s0040 | 58.228 | 79 | 11 | 1 | 195 | 273 | 15 | 71 | 1.17e-22 | 89.7 |
| MS.gene44015.t1 | MTR_0716s0020 | 71.154 | 52 | 11 | 1 | 56 | 107 | 3 | 50 | 3.62e-16 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44015.t1 | AT5G28150 | 65.789 | 304 | 88 | 4 | 1 | 303 | 1 | 289 | 1.66e-139 | 395 |
| MS.gene44015.t1 | AT3G04860 | 64.379 | 306 | 89 | 6 | 1 | 303 | 1 | 289 | 4.65e-135 | 384 |
| MS.gene44015.t1 | AT2G04220 | 41.200 | 250 | 142 | 3 | 14 | 259 | 12 | 260 | 2.02e-65 | 207 |
| MS.gene44015.t1 | AT4G12690 | 41.975 | 243 | 135 | 4 | 15 | 253 | 9 | 249 | 3.14e-64 | 204 |
| MS.gene44015.t1 | AT4G12690 | 41.975 | 243 | 135 | 4 | 15 | 253 | 9 | 249 | 3.14e-64 | 204 |
| MS.gene44015.t1 | AT5G48270 | 36.260 | 262 | 149 | 9 | 5 | 253 | 13 | 269 | 7.46e-52 | 173 |
| MS.gene44015.t1 | AT2G27770 | 33.433 | 335 | 173 | 10 | 1 | 301 | 1 | 319 | 2.00e-49 | 167 |
| MS.gene44015.t1 | AT3G13229 | 34.894 | 235 | 142 | 4 | 33 | 256 | 8 | 242 | 3.79e-45 | 155 |
| MS.gene44015.t1 | AT5G11000 | 37.302 | 252 | 117 | 9 | 31 | 253 | 33 | 272 | 7.76e-43 | 151 |
| MS.gene44015.t1 | AT2G25200 | 28.571 | 259 | 147 | 8 | 6 | 242 | 16 | 258 | 1.32e-30 | 118 |
| MS.gene44015.t1 | AT2G36470 | 26.471 | 272 | 145 | 8 | 1 | 241 | 1 | 248 | 9.25e-20 | 88.2 |
Find 43 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCTCTCAGGATAAAATT+TGG | 0.148503 | 1.3:-57464645 | MS.gene44015:CDS |
| AAACAAATGGTTCTTCTTCT+TGG | 0.224208 | 1.3:-57465027 | MS.gene44015:CDS |
| TTTATTGGGATGTTTATAAT+TGG | 0.245969 | 1.3:-57464714 | MS.gene44015:CDS |
| GTCTTGAAGTTGAGGTTTAT+TGG | 0.251621 | 1.3:-57464729 | MS.gene44015:CDS |
| AACACCTTGTTGCTGCAATT+TGG | 0.305444 | 1.3:+57464558 | None:intergenic |
| GATCTTTCTTCAGCTAGATT+TGG | 0.328970 | 1.3:-57465099 | MS.gene44015:CDS |
| TTTCTCTCAGGATAAAATTT+GGG | 0.343648 | 1.3:-57464644 | MS.gene44015:CDS |
| GTTTACCAGTGTTCAATTAG+AGG | 0.364536 | 1.3:-57465303 | MS.gene44015:CDS |
| AGGTTGAAGTGGAAGTTTAG+AGG | 0.371290 | 1.3:-57464775 | MS.gene44015:CDS |
| TCTTGAAGTTGAGGTTTATT+GGG | 0.375109 | 1.3:-57464728 | MS.gene44015:CDS |
| TTCTTCTTCTTGGTGATTTG+AGG | 0.384951 | 1.3:-57465017 | MS.gene44015:CDS |
| AAAACATTTGCATCAGAAAC+AGG | 0.388646 | 1.3:+57464613 | None:intergenic |
| TTCAAGACCATCCACAAGTA+TGG | 0.397921 | 1.3:+57464744 | None:intergenic |
| AAGCAAAACTGCTCAGAATC+TGG | 0.403487 | 1.3:-57465334 | MS.gene44015:CDS |
| GTTGTAAGATTGATGTGTAT+TGG | 0.407371 | 1.3:-57465122 | MS.gene44015:CDS |
| CTTGCTTGATTACTATTACA+TGG | 0.414580 | 1.3:-57465275 | MS.gene44015:CDS |
| GTGATCCTTGTCTTATAGTT+CGG | 0.427025 | 1.3:-57464828 | MS.gene44015:CDS |
| TTGTAAGATTGATGTGTATT+GGG | 0.428969 | 1.3:-57465121 | MS.gene44015:CDS |
| GTTTAGAACATGTTTCTCTC+AGG | 0.471644 | 1.3:-57464656 | MS.gene44015:CDS |
| TCTTGGTGATTTGAGGAAAG+AGG | 0.473028 | 1.3:-57465010 | MS.gene44015:CDS |
| TGTAGTTGTTGATAAACAAA+TGG | 0.479417 | 1.3:-57465040 | MS.gene44015:CDS |
| TAAAGTTGATATAAAACCTT+GGG | 0.481774 | 1.3:-57465187 | MS.gene44015:CDS |
| TACATGGAGTAAGAGTTTGA+TGG | 0.485899 | 1.3:-57465259 | MS.gene44015:CDS |
| TCTTCAGCTAGATTTGGTGT+TGG | 0.510529 | 1.3:-57465093 | MS.gene44015:CDS |
| TGTGGATGGTCTTGAAGTTG+AGG | 0.522288 | 1.3:-57464737 | MS.gene44015:CDS |
| AAAACAGCATTCAAAGGTAA+AGG | 0.522755 | 1.3:+57464964 | None:intergenic |
| CGATAGCAAAACTGTGATGC+AGG | 0.523931 | 1.3:-57464803 | MS.gene44015:CDS |
| AGTAAGAGTTTGATGGGTCA+AGG | 0.525556 | 1.3:-57465252 | MS.gene44015:CDS |
| ACATGGAGTAAGAGTTTGAT+GGG | 0.527449 | 1.3:-57465258 | MS.gene44015:CDS |
| TGCAGTGGTCTTTCTCTCAG+AGG | 0.536201 | 1.3:-57464591 | MS.gene44015:CDS |
| TGCAGGTGAAGAGGTTGAAG+TGG | 0.541978 | 1.3:-57464786 | MS.gene44015:CDS |
| TGTTGGACCTGAACCATTGG+AGG | 0.554106 | 1.3:-57465076 | MS.gene44015:CDS |
| TGGTGTTGGACCTGAACCAT+TGG | 0.554528 | 1.3:-57465079 | MS.gene44015:CDS |
| GTAAAGTTGATATAAAACCT+TGG | 0.555894 | 1.3:-57465188 | MS.gene44015:CDS |
| AAATTGCAGCAACAAGGTGT+TGG | 0.560217 | 1.3:-57464556 | MS.gene44015:CDS |
| AGGAAATCATACCATACTTG+TGG | 0.576851 | 1.3:-57464755 | MS.gene44015:CDS |
| GTTGGACCTGAACCATTGGA+GGG | 0.614502 | 1.3:-57465075 | MS.gene44015:CDS |
| AATCATACCATACTTGTGGA+TGG | 0.616419 | 1.3:-57464751 | MS.gene44015:CDS |
| ATCTACCTCTAATTGAACAC+TGG | 0.618529 | 1.3:+57465298 | None:intergenic |
| GAGACCAAATTGCAGCAACA+AGG | 0.620010 | 1.3:-57464562 | MS.gene44015:CDS |
| TTTCTCTTAGAAAACACCCA+AGG | 0.637931 | 1.3:+57465171 | None:intergenic |
| AAACTGTGATGCAGGTGAAG+AGG | 0.662598 | 1.3:-57464795 | MS.gene44015:CDS |
| TCGATCCGAACTATAAGACA+AGG | 0.676375 | 1.3:+57464823 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATTGGGATGTTTATAAT+TGG | - | chr1.3:57465213-57465232 | MS.gene44015:CDS | 20.0% |
| !!! | TAAAGTTGATATAAAACCTT+GGG | - | chr1.3:57464740-57464759 | MS.gene44015:CDS | 20.0% |
| ! | TTGTAAGATTGATGTGTATT+GGG | - | chr1.3:57464806-57464825 | MS.gene44015:CDS | 25.0% |
| ! | TTTCTCTCAGGATAAAATTT+GGG | - | chr1.3:57465283-57465302 | MS.gene44015:CDS | 25.0% |
| !! | TGTAGTTGTTGATAAACAAA+TGG | - | chr1.3:57464887-57464906 | MS.gene44015:CDS | 25.0% |
| !!! | GATGTTTATAATTGGCTTTT+TGG | - | chr1.3:57465221-57465240 | MS.gene44015:CDS | 25.0% |
| !!! | GTAAAGTTGATATAAAACCT+TGG | - | chr1.3:57464739-57464758 | MS.gene44015:CDS | 25.0% |
| !!! | GTTTTTGGTAAGAAGTTTTT+TGG | - | chr1.3:57465005-57465024 | MS.gene44015:CDS | 25.0% |
| !!! | TAAGAAGTTTTTTGGTAACA+AGG | - | chr1.3:57465013-57465032 | MS.gene44015:CDS | 25.0% |
| AAAACAGCATTCAAAGGTAA+AGG | + | chr1.3:57464966-57464985 | None:intergenic | 30.0% | |
| AAAACATTTGCATCAGAAAC+AGG | + | chr1.3:57465317-57465336 | None:intergenic | 30.0% | |
| AAACAAATGGTTCTTCTTCT+TGG | - | chr1.3:57464900-57464919 | MS.gene44015:CDS | 30.0% | |
| CTTGCTTGATTACTATTACA+TGG | - | chr1.3:57464652-57464671 | MS.gene44015:CDS | 30.0% | |
| GTTGTAAGATTGATGTGTAT+TGG | - | chr1.3:57464805-57464824 | MS.gene44015:CDS | 30.0% | |
| GTTTCTCTCAGGATAAAATT+TGG | - | chr1.3:57465282-57465301 | MS.gene44015:CDS | 30.0% | |
| TCTTGAAGTTGAGGTTTATT+GGG | - | chr1.3:57465199-57465218 | MS.gene44015:CDS | 30.0% | |
| ! | AAGGTTGTGTTTTGTGATAA+TGG | - | chr1.3:57465032-57465051 | MS.gene44015:CDS | 30.0% |
| ! | GCTAAGAAAGAACATGTTTT+TGG | - | chr1.3:57464990-57465009 | MS.gene44015:CDS | 30.0% |
| !! | AAGGATTTTCCATCTTGTTT+TGG | - | chr1.3:57464522-57464541 | MS.gene44015:CDS | 30.0% |
| AATCATACCATACTTGTGGA+TGG | - | chr1.3:57465176-57465195 | MS.gene44015:CDS | 35.0% | |
| ACATGGAGTAAGAGTTTGAT+GGG | - | chr1.3:57464669-57464688 | MS.gene44015:CDS | 35.0% | |
| AGGAAATCATACCATACTTG+TGG | - | chr1.3:57465172-57465191 | MS.gene44015:CDS | 35.0% | |
| ATCTACCTCTAATTGAACAC+TGG | + | chr1.3:57464632-57464651 | None:intergenic | 35.0% | |
| GATCTTTCTTCAGCTAGATT+TGG | - | chr1.3:57464828-57464847 | MS.gene44015:CDS | 35.0% | |
| GCAACAAAAACAGCATTCAA+AGG | + | chr1.3:57464972-57464991 | None:intergenic | 35.0% | |
| GTCTTGAAGTTGAGGTTTAT+TGG | - | chr1.3:57465198-57465217 | MS.gene44015:CDS | 35.0% | |
| GTGATCCTTGTCTTATAGTT+CGG | - | chr1.3:57465099-57465118 | MS.gene44015:CDS | 35.0% | |
| GTTTACCAGTGTTCAATTAG+AGG | - | chr1.3:57464624-57464643 | MS.gene44015:CDS | 35.0% | |
| TACATGGAGTAAGAGTTTGA+TGG | - | chr1.3:57464668-57464687 | MS.gene44015:CDS | 35.0% | |
| TTTCTCTTAGAAAACACCCA+AGG | + | chr1.3:57464759-57464778 | None:intergenic | 35.0% | |
| ! | GCATTTTCACCAAAACAAGA+TGG | + | chr1.3:57464534-57464553 | None:intergenic | 35.0% |
| ! | GTTTAGAACATGTTTCTCTC+AGG | - | chr1.3:57465271-57465290 | MS.gene44015:CDS | 35.0% |
| ! | TCTCACATGTTTTGTATGCT+TGG | - | chr1.3:57465396-57465415 | MS.gene44015:CDS | 35.0% |
| ! | TTCTTCTTCTTGGTGATTTG+AGG | - | chr1.3:57464910-57464929 | MS.gene44015:CDS | 35.0% |
| ! | TTTTGGTGAAAATGCAGTTC+AGG | - | chr1.3:57464539-57464558 | MS.gene44015:CDS | 35.0% |
| !! | TGGCTTTTTGGTACATCTTT+TGG | - | chr1.3:57465233-57465252 | MS.gene44015:CDS | 35.0% |
| !! | TGGGTGTTTTCTAAGAGAAA+GGG | - | chr1.3:57464759-57464778 | MS.gene44015:CDS | 35.0% |
| !! | TTGGGTGTTTTCTAAGAGAA+AGG | - | chr1.3:57464758-57464777 | MS.gene44015:CDS | 35.0% |
| AAAAAACCCTCCAATGGTTC+AGG | + | chr1.3:57464861-57464880 | None:intergenic | 40.0% | |
| AAATTGCAGCAACAAGGTGT+TGG | - | chr1.3:57465371-57465390 | MS.gene44015:CDS | 40.0% | |
| AACACCTTGTTGCTGCAATT+TGG | + | chr1.3:57465372-57465391 | None:intergenic | 40.0% | |
| AAGCAAAACTGCTCAGAATC+TGG | - | chr1.3:57464593-57464612 | MS.gene44015:CDS | 40.0% | |
| AGGTTGAAGTGGAAGTTTAG+AGG | - | chr1.3:57465152-57465171 | MS.gene44015:CDS | 40.0% | |
| CCTACAAAAAAACCCTCCAA+TGG | + | chr1.3:57464867-57464886 | None:intergenic | 40.0% | |
| TCGATCCGAACTATAAGACA+AGG | + | chr1.3:57465107-57465126 | None:intergenic | 40.0% | |
| TTCAAGACCATCCACAAGTA+TGG | + | chr1.3:57465186-57465205 | None:intergenic | 40.0% | |
| ! | AGTAAGAGTTTGATGGGTCA+AGG | - | chr1.3:57464675-57464694 | MS.gene44015:CDS | 40.0% |
| ! | CTGATGCAAATGTTTTGCAG+TGG | - | chr1.3:57465321-57465340 | MS.gene44015:CDS | 40.0% |
| ! | TCTTGGTGATTTGAGGAAAG+AGG | - | chr1.3:57464917-57464936 | MS.gene44015:CDS | 40.0% |
| !! | ATGGGTCAAGGTTTTAGTGT+TGG | - | chr1.3:57464687-57464706 | MS.gene44015:CDS | 40.0% |
| !! | TCTTCAGCTAGATTTGGTGT+TGG | - | chr1.3:57464834-57464853 | MS.gene44015:CDS | 40.0% |
| !! | TGGGTCAAGGTTTTAGTGTT+GGG | - | chr1.3:57464688-57464707 | MS.gene44015:CDS | 40.0% |
| !!! | CCATTGGAGGGTTTTTTTGT+AGG | - | chr1.3:57464864-57464883 | MS.gene44015:CDS | 40.0% |
| AAACTGTGATGCAGGTGAAG+AGG | - | chr1.3:57465132-57465151 | MS.gene44015:CDS | 45.0% | |
| CGATAGCAAAACTGTGATGC+AGG | - | chr1.3:57465124-57465143 | MS.gene44015:CDS | 45.0% | |
| GAGACCAAATTGCAGCAACA+AGG | - | chr1.3:57465365-57465384 | MS.gene44015:CDS | 45.0% | |
| ! | TGTGGATGGTCTTGAAGTTG+AGG | - | chr1.3:57465190-57465209 | MS.gene44015:CDS | 45.0% |
| GTTGGACCTGAACCATTGGA+GGG | - | chr1.3:57464852-57464871 | MS.gene44015:CDS | 50.0% | |
| TGCAGGTGAAGAGGTTGAAG+TGG | - | chr1.3:57465141-57465160 | MS.gene44015:CDS | 50.0% | |
| TGCAGTGGTCTTTCTCTCAG+AGG | - | chr1.3:57465336-57465355 | MS.gene44015:CDS | 50.0% | |
| TGTTGGACCTGAACCATTGG+AGG | - | chr1.3:57464851-57464870 | MS.gene44015:CDS | 50.0% | |
| ! | TGGTGTTGGACCTGAACCAT+TGG | - | chr1.3:57464848-57464867 | MS.gene44015:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 57464519 | 57465430 | 57464519 | ID=MS.gene44015 |
| chr1.3 | mRNA | 57464519 | 57465430 | 57464519 | ID=MS.gene44015.t1;Parent=MS.gene44015 |
| chr1.3 | exon | 57464519 | 57465430 | 57464519 | ID=MS.gene44015.t1.exon1;Parent=MS.gene44015.t1 |
| chr1.3 | CDS | 57464519 | 57465430 | 57464519 | ID=cds.MS.gene44015.t1;Parent=MS.gene44015.t1 |
| Gene Sequence |
| Protein sequence |
