AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44180.t1 MTR_1g083950 100.000 165 0 0 1 165 1 165 2.71e-123 343
MS.gene44180.t1 MTR_1g087200 53.247 154 66 3 9 157 6 158 4.06e-56 174
MS.gene44180.t1 MTR_1g054750 40.741 162 83 3 10 158 23 184 2.52e-34 119
MS.gene44180.t1 MTR_1g054765 38.690 168 84 4 10 158 4 171 1.10e-32 114
MS.gene44180.t1 MTR_1g088640 40.132 152 88 2 11 159 14 165 2.85e-32 113
MS.gene44180.t1 MTR_1g088640 40.132 152 85 3 11 159 14 162 4.16e-31 110
MS.gene44180.t1 MTR_5g065980 37.107 159 86 4 11 155 10 168 3.79e-30 108
MS.gene44180.t1 MTR_5g065980 37.037 162 84 5 11 155 10 170 3.87e-30 108
MS.gene44180.t1 MTR_7g108830 40.123 162 90 4 1 158 1 159 4.98e-27 100
MS.gene44180.t1 MTR_8g058620 40.123 162 90 4 1 158 1 159 4.98e-27 100
MS.gene44180.t1 MTR_2g089350 30.539 167 102 4 9 163 25 189 1.39e-21 87.4
MS.gene44180.t1 MTR_5g077130 34.591 159 89 6 13 160 9 163 3.91e-19 79.7
MS.gene44180.t1 MTR_5g077130 33.962 159 89 5 13 160 9 162 8.50e-19 79.0
MS.gene44180.t1 MTR_1g088620 55.224 67 30 0 93 159 218 284 5.14e-18 79.0
MS.gene44180.t1 MTR_5g065980 32.374 139 80 4 11 135 10 148 8.26e-18 77.0
MS.gene44180.t1 MTR_2g034200 31.250 160 94 4 7 155 3 157 1.56e-17 75.5
MS.gene44180.t1 MTR_6g006190 27.485 171 110 5 6 164 3 171 4.19e-17 74.7
MS.gene44180.t1 MTR_4g019800 34.194 155 92 4 9 155 5 157 2.17e-16 72.4
MS.gene44180.t1 MTR_4g064933 31.902 163 101 4 7 161 3 163 4.80e-14 66.2
MS.gene44180.t1 MTR_6g006190 36.842 76 47 1 90 164 72 147 1.20e-12 62.4
MS.gene44180.t1 MTR_7g065770 31.613 155 96 4 9 155 5 157 1.61e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44180.t1 AT3G62550 50.993 151 71 1 9 156 7 157 5.62e-52 163
MS.gene44180.t1 AT3G11930 38.922 167 88 4 9 161 33 199 1.80e-34 120
MS.gene44180.t1 AT3G11930 39.157 166 88 4 9 161 33 198 2.04e-34 120
MS.gene44180.t1 AT3G11930 38.690 168 88 5 9 161 33 200 9.87e-34 118
MS.gene44180.t1 AT3G58450 38.650 163 88 4 11 161 32 194 7.65e-32 113
MS.gene44180.t1 AT3G58450 39.375 160 84 3 11 161 32 187 3.91e-31 111
MS.gene44180.t1 AT1G09740 37.654 162 91 3 11 162 10 171 1.21e-30 109
MS.gene44180.t1 AT1G09740 37.654 162 91 3 11 162 10 171 1.21e-30 109
MS.gene44180.t1 AT2G47710 36.875 160 92 4 5 158 3 159 6.37e-30 107
MS.gene44180.t1 AT3G11930 33.679 193 88 5 9 161 33 225 3.50e-28 104
MS.gene44180.t1 AT1G68300 36.242 149 92 3 9 155 10 157 5.29e-27 99.8
MS.gene44180.t1 AT3G17020 36.025 161 84 6 8 155 6 160 2.92e-20 82.4
MS.gene44180.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene44180.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene44180.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene44180.t1 AT1G11360 29.586 169 104 5 9 165 38 203 2.31e-18 79.3
MS.gene44180.t1 AT3G01520 31.944 144 93 4 24 164 30 171 1.52e-17 75.9
MS.gene44180.t1 AT5G14680 29.940 167 103 5 10 164 7 171 8.73e-17 73.9
MS.gene44180.t1 AT3G03270 30.818 159 94 5 8 155 4 157 1.99e-15 69.7
MS.gene44180.t1 AT3G53990 28.302 159 96 4 9 155 5 157 3.11e-12 61.6
MS.gene44180.t1 AT3G25930 29.012 162 87 8 9 156 2 149 3.16e-11 58.5

Find 44 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTCCAGTGGTTGATTT+TGG 0.145041 1.3:-59584796 None:intergenic
CACATGGTGCCTCAAGAATT+TGG 0.151045 1.3:+59584084 MS.gene44180:CDS
GTTGTTTATTCTGCCTTTGA+TGG 0.302678 1.3:+59584160 MS.gene44180:CDS
GGCTTCTTCACAATCAAAAC+AGG 0.346785 1.3:-59584775 None:intergenic
ACAAGTTGCTGATTGTGTTT+TGG 0.353665 1.3:+59584329 MS.gene44180:CDS
GGGTGTTGATATCTTGGTTA+TGG 0.361677 1.3:+59584556 MS.gene44180:CDS
GAGGAGGATAAGGTGATCTT+TGG 0.363644 1.3:-59584119 None:intergenic
GACATCATTACAGACTATCT+TGG 0.412460 1.3:-59584358 None:intergenic
GGTGTTGATATCTTGGTTAT+GGG 0.433422 1.3:+59584557 MS.gene44180:CDS
AAACACAATCAGCAACTTGT+TGG 0.448947 1.3:-59584326 None:intergenic
CCGTTATGTCAGAAGAGAAT+AGG 0.454640 1.3:-59584284 None:intergenic
CCCTAGGGATGTAATCTGTC+AGG 0.458933 1.3:+59584520 MS.gene44180:CDS
TATTCTGCCTTTGATGGCAC+AGG 0.479279 1.3:+59584166 MS.gene44180:CDS
TTGAATGGCAGGGCTTTCCT+TGG 0.488782 1.3:+59584715 MS.gene44180:intron
ATCTTAATTGTCTCCTCCAG+TGG 0.492223 1.3:-59584806 None:intergenic
TTGTTGGCTATACTTCTCCA+TGG 0.495342 1.3:-59584310 None:intergenic
GCTTAACATAGAGGAGGATA+AGG 0.496830 1.3:-59584129 None:intergenic
ATGGCTGGTATAACAGAAAA+CGG 0.497259 1.3:+59584010 MS.gene44180:CDS
GTGGAGACGAGGATAGAGAA+TGG 0.517274 1.3:+59584494 MS.gene44180:CDS
GTTATGGGAAGCCACGGCTA+TGG 0.518096 1.3:+59584572 MS.gene44180:CDS
GGATAGAGAATGGTGACCCT+AGG 0.527492 1.3:+59584504 MS.gene44180:CDS
CCTATTCTCTTCTGACATAA+CGG 0.542181 1.3:+59584284 MS.gene44180:CDS
ATCAAGCAGTGTGAAATGGC+TGG 0.546216 1.3:+59583995 None:intergenic
ATCTTGGTTATGGGAAGCCA+CGG 0.562584 1.3:+59584566 MS.gene44180:CDS
AGAAAACGGAAGAAGAATCA+TGG 0.569970 1.3:+59584024 MS.gene44180:CDS
CACGAGGAGGCTTAACATAG+AGG 0.577003 1.3:-59584138 None:intergenic
AGTGGTTGCTCACACTTCCA+AGG 0.579050 1.3:-59584732 None:intergenic
TTAATTACCTGTGCCATCAA+AGG 0.579397 1.3:-59584173 None:intergenic
GATAGAGAATGGTGACCCTA+GGG 0.580628 1.3:+59584505 MS.gene44180:CDS
AAGAAGCCAAAATCAACCAC+TGG 0.584975 1.3:+59584790 MS.gene44180:CDS
GCCTGACAGATTACATCCCT+AGG 0.585000 1.3:-59584521 None:intergenic
ATCATGGTAGCTATTGATGA+AGG 0.586206 1.3:+59584040 MS.gene44180:CDS
TTCTGACATAACGGCAACCA+TGG 0.586507 1.3:+59584293 MS.gene44180:CDS
ACGGCTATGGTGTCATCAAG+AGG 0.638531 1.3:+59584585 MS.gene44180:CDS
AAGCCAAAATCAACCACTGG+AGG 0.644827 1.3:+59584793 MS.gene44180:CDS
GCTTCTTCACAATCAAAACA+GGG 0.652447 1.3:-59584774 None:intergenic
ATATGGTTTAGGTGGAGACG+AGG 0.658827 1.3:+59584483 MS.gene44180:intron
CCTGACAGATTACATCCCTA+GGG 0.659449 1.3:-59584520 None:intergenic
AGAGCATTTATGCTCTCACA+TGG 0.669047 1.3:+59584068 MS.gene44180:CDS
TCTTGATGACACCATAGCCG+TGG 0.679887 1.3:-59584583 None:intergenic
ACTTCACATTCTGTGCACAG+TGG 0.689187 1.3:-59584750 None:intergenic
GCAGAATAAACAACACGAGG+AGG 0.692643 1.3:-59584151 None:intergenic
GAGGAGGCTTAACATAGAGG+AGG 0.704073 1.3:-59584135 None:intergenic
AAGGCAGAATAAACAACACG+AGG 0.733599 1.3:-59584154 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAACTATAGATAAAAATG+AGG - chr1.3:59584443-59584462 None:intergenic 15.0%
!! TGTGAATTGTAAATTTAATT+TGG + chr1.3:59584677-59584696 MS.gene44180:intron 15.0%
!! TTAATAATTAATCTGTGATA+TGG + chr1.3:59584466-59584485 MS.gene44180:intron 15.0%
!! TTGTATCATATATATGTTTC+AGG + chr1.3:59584258-59584277 MS.gene44180:intron 20.0%
!!! TCAAATGTATACTTACATTT+TGG - chr1.3:59584383-59584402 None:intergenic 20.0%
!!! TTGTAAATTTAATTTGGTTC+TGG + chr1.3:59584683-59584702 MS.gene44180:intron 20.0%
! AACTATAGATAAAAATGAGG+AGG - chr1.3:59584440-59584459 None:intergenic 25.0%
! ACTATAGATAAAAATGAGGA+GGG - chr1.3:59584439-59584458 None:intergenic 25.0%
! ATTAATCTGTGATATGGTTT+AGG + chr1.3:59584472-59584491 MS.gene44180:intron 25.0%
! TTTGAAAAACCAAATTCTTG+AGG - chr1.3:59584096-59584115 None:intergenic 25.0%
!! ATTTTTGTTAGAATGAGCTA+AGG + chr1.3:59584653-59584672 MS.gene44180:intron 25.0%
!! AAAAATGGGTGTTGATATCT+TGG + chr1.3:59584550-59584569 MS.gene44180:CDS 30.0%
AAACACAATCAGCAACTTGT+TGG - chr1.3:59584329-59584348 None:intergenic 35.0%
AATCTGTGATATGGTTTAGG+TGG + chr1.3:59584475-59584494 MS.gene44180:intron 35.0%
AGAAAACGGAAGAAGAATCA+TGG + chr1.3:59584024-59584043 MS.gene44180:CDS 35.0%
ATCATGGTAGCTATTGATGA+AGG + chr1.3:59584040-59584059 MS.gene44180:CDS 35.0%
ATGGCTGGTATAACAGAAAA+CGG + chr1.3:59584010-59584029 MS.gene44180:CDS 35.0%
CCTATTCTCTTCTGACATAA+CGG + chr1.3:59584284-59584303 MS.gene44180:CDS 35.0%
GACATCATTACAGACTATCT+TGG - chr1.3:59584361-59584380 None:intergenic 35.0%
GCTTCTTCACAATCAAAACA+GGG - chr1.3:59584777-59584796 None:intergenic 35.0%
GTTGTTTATTCTGCCTTTGA+TGG + chr1.3:59584160-59584179 MS.gene44180:CDS 35.0%
TGTCAGGCTGTTCAAAAAAT+GGG + chr1.3:59584536-59584555 MS.gene44180:CDS 35.0%
TTAATTACCTGTGCCATCAA+AGG - chr1.3:59584176-59584195 None:intergenic 35.0%
! TTCTGGCTGTTATTGTTGAA+TGG + chr1.3:59584700-59584719 MS.gene44180:intron 35.0%
!! ACAAGTTGCTGATTGTGTTT+TGG + chr1.3:59584329-59584348 MS.gene44180:CDS 35.0%
!! GGTGTTGATATCTTGGTTAT+GGG + chr1.3:59584557-59584576 MS.gene44180:CDS 35.0%
AAGAAGCCAAAATCAACCAC+TGG + chr1.3:59584790-59584809 MS.gene44180:CDS 40.0%
AAGGCAGAATAAACAACACG+AGG - chr1.3:59584157-59584176 None:intergenic 40.0%
CCGTTATGTCAGAAGAGAAT+AGG - chr1.3:59584287-59584306 None:intergenic 40.0%
CTGTCAGGCTGTTCAAAAAA+TGG + chr1.3:59584535-59584554 MS.gene44180:CDS 40.0%
GCTGTTATTGTTGAATGGCA+GGG + chr1.3:59584705-59584724 MS.gene44180:intron 40.0%
GCTTAACATAGAGGAGGATA+AGG - chr1.3:59584132-59584151 None:intergenic 40.0%
GGCTTCTTCACAATCAAAAC+AGG - chr1.3:59584778-59584797 None:intergenic 40.0%
! AGAGCATTTATGCTCTCACA+TGG + chr1.3:59584068-59584087 MS.gene44180:CDS 40.0%
! TTGTTGGCTATACTTCTCCA+TGG - chr1.3:59584313-59584332 None:intergenic 40.0%
!! GGGTGTTGATATCTTGGTTA+TGG + chr1.3:59584556-59584575 MS.gene44180:CDS 40.0%
AAGCCAAAATCAACCACTGG+AGG + chr1.3:59584793-59584812 MS.gene44180:CDS 45.0%
ACTTCACATTCTGTGCACAG+TGG - chr1.3:59584753-59584772 None:intergenic 45.0%
ATATGGTTTAGGTGGAGACG+AGG + chr1.3:59584483-59584502 MS.gene44180:intron 45.0%
ATCTTGGTTATGGGAAGCCA+CGG + chr1.3:59584566-59584585 MS.gene44180:CDS 45.0%
CACATGGTGCCTCAAGAATT+TGG + chr1.3:59584084-59584103 MS.gene44180:CDS 45.0%
CCTGACAGATTACATCCCTA+GGG - chr1.3:59584523-59584542 None:intergenic 45.0%
GAGGAGGATAAGGTGATCTT+TGG - chr1.3:59584122-59584141 None:intergenic 45.0%
GATAGAGAATGGTGACCCTA+GGG + chr1.3:59584505-59584524 MS.gene44180:CDS 45.0%
GCAGAATAAACAACACGAGG+AGG - chr1.3:59584154-59584173 None:intergenic 45.0%
GGCTGTTATTGTTGAATGGC+AGG + chr1.3:59584704-59584723 MS.gene44180:intron 45.0%
TTCTGACATAACGGCAACCA+TGG + chr1.3:59584293-59584312 MS.gene44180:CDS 45.0%
! TATTCTGCCTTTGATGGCAC+AGG + chr1.3:59584166-59584185 MS.gene44180:CDS 45.0%
!! TCTCCTCCAGTGGTTGATTT+TGG - chr1.3:59584799-59584818 None:intergenic 45.0%
AGTGGTTGCTCACACTTCCA+AGG - chr1.3:59584735-59584754 None:intergenic 50.0%
CACGAGGAGGCTTAACATAG+AGG - chr1.3:59584141-59584160 None:intergenic 50.0%
CCCTAGGGATGTAATCTGTC+AGG + chr1.3:59584520-59584539 MS.gene44180:CDS 50.0%
GAGGAGGCTTAACATAGAGG+AGG - chr1.3:59584138-59584157 None:intergenic 50.0%
GCCTGACAGATTACATCCCT+AGG - chr1.3:59584524-59584543 None:intergenic 50.0%
GGATAGAGAATGGTGACCCT+AGG + chr1.3:59584504-59584523 MS.gene44180:CDS 50.0%
GTGGAGACGAGGATAGAGAA+TGG + chr1.3:59584494-59584513 MS.gene44180:CDS 50.0%
TTGAATGGCAGGGCTTTCCT+TGG + chr1.3:59584715-59584734 MS.gene44180:intron 50.0%
! TCTTGATGACACCATAGCCG+TGG - chr1.3:59584586-59584605 None:intergenic 50.0%
!! ACGGCTATGGTGTCATCAAG+AGG + chr1.3:59584585-59584604 MS.gene44180:CDS 50.0%
GTTATGGGAAGCCACGGCTA+TGG + chr1.3:59584572-59584591 MS.gene44180:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59584010 59584825 59584010 ID=MS.gene44180
chr1.3 mRNA 59584010 59584825 59584010 ID=MS.gene44180.t1;Parent=MS.gene44180
chr1.3 exon 59584010 59584187 59584010 ID=MS.gene44180.t1.exon1;Parent=MS.gene44180.t1
chr1.3 CDS 59584010 59584187 59584010 ID=cds.MS.gene44180.t1;Parent=MS.gene44180.t1
chr1.3 exon 59584280 59584386 59584280 ID=MS.gene44180.t1.exon2;Parent=MS.gene44180.t1
chr1.3 CDS 59584280 59584386 59584280 ID=cds.MS.gene44180.t1;Parent=MS.gene44180.t1
chr1.3 exon 59584494 59584606 59584494 ID=MS.gene44180.t1.exon3;Parent=MS.gene44180.t1
chr1.3 CDS 59584494 59584606 59584494 ID=cds.MS.gene44180.t1;Parent=MS.gene44180.t1
chr1.3 exon 59584726 59584825 59584726 ID=MS.gene44180.t1.exon4;Parent=MS.gene44180.t1
chr1.3 CDS 59584726 59584825 59584726 ID=cds.MS.gene44180.t1;Parent=MS.gene44180.t1
Gene Sequence

>MS.gene44180

ATGGCTGGTATAACAGAAAACGGAAGAAGAATCATGGTAGCTATTGATGAAGGTGAAGAGAGCATTTATGCTCTCACATGGTGCCTCAAGAATTTGGTTTTTCAAAATTCCAAAGATCACCTTATCCTCCTCTATGTTAAGCCTCCTCGTGTTGTTTATTCTGCCTTTGATGGCACAGGTAATTAAACAAAATTTTATGATACGTAGCAAATAATTTTCTATTAATTTTGTAAACTCATAATTAATTTTTGTATCATATATATGTTTCAGGATACCTATTCTCTTCTGACATAACGGCAACCATGGAGAAGTATAGCCAACAAGTTGCTGATTGTGTTTTGGAGAAAGCCAAGATAGTCTGTAATGATGTCCAAAATGTAAGTATACATTTGATCAATTGTTACTAGTTAGATATGTATATTATGTCCCTCCTCATTTTTATCTATAGTTAATTATTTAATAATTAATCTGTGATATGGTTTAGGTGGAGACGAGGATAGAGAATGGTGACCCTAGGGATGTAATCTGTCAGGCTGTTCAAAAAATGGGTGTTGATATCTTGGTTATGGGAAGCCACGGCTATGGTGTCATCAAGAGGTCAGTTTTTGATAATTCATACAATACATATTTGAATTGTAAAGTTATTTTTGTTAGAATGAGCTAAGGATGTGAATTGTAAATTTAATTTGGTTCTGGCTGTTATTGTTGAATGGCAGGGCTTTCCTTGGAAGTGTGAGCAACCACTGTGCACAGAATGTGAAGTGCCCTGTTTTGATTGTGAAGAAGCCAAAATCAACCACTGGAGGAGACAATTAA

Protein sequence

>MS.gene44180.t1

MAGITENGRRIMVAIDEGEESIYALTWCLKNLVFQNSKDHLILLYVKPPRVVYSAFDGTGYLFSSDITATMEKYSQQVADCVLEKAKIVCNDVQNVETRIENGDPRDVICQAVQKMGVDILVMGSHGYGVIKRAFLGSVSNHCAQNVKCPVLIVKKPKSTTGGDN