Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46359.t1 | PNX85593.1 | 88.6 | 114 | 13 | 0 | 1 | 114 | 1 | 114 | 4.70E-51 | 210.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46359.t1 | Q9LVZ7 | 78.1 | 105 | 23 | 0 | 1 | 105 | 176 | 280 | 3.8e-41 | 168.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46359.t1 | A0A2K3M4A9 | 88.6 | 114 | 13 | 0 | 1 | 114 | 1 | 114 | 3.4e-51 | 210.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050026 | MS.gene46359 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene46359 | 0.812335 | 4.46E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene46359 | 0.815214 | 1.04E-51 | -1.69E-46 |
| MS.gene054801 | MS.gene46359 | 0.802415 | 5.67E-49 | -1.69E-46 |
| MS.gene055091 | MS.gene46359 | 0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene46359 | 0.840879 | 6.66E-58 | -1.69E-46 |
| MS.gene059272 | MS.gene46359 | 0.824829 | 6.53E-54 | -1.69E-46 |
| MS.gene059276 | MS.gene46359 | 0.840795 | 7.01E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46359.t1 | MTR_4g010120 | 90.351 | 114 | 11 | 0 | 1 | 114 | 154 | 267 | 4.35e-69 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46359.t1 | AT3G15820 | 78.095 | 105 | 23 | 0 | 1 | 105 | 176 | 280 | 9.44e-54 | 170 |
| MS.gene46359.t1 | AT3G15830 | 74.074 | 108 | 28 | 0 | 1 | 108 | 171 | 278 | 8.59e-53 | 167 |
Find 21 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATGAATCACCTGAGGAAA+TGG | 0.322423 | 8.4:-24213902 | None:intergenic |
| AGGCTATTACACTATTGATT+TGG | 0.328688 | 8.4:+24214706 | MS.gene46359:CDS |
| TTGGGGTCATGTGGCGGGTT+CGG | 0.337656 | 8.4:+24214583 | MS.gene46359:CDS |
| TACACTATTGATTTGGCTGT+TGG | 0.339883 | 8.4:+24214713 | MS.gene46359:CDS |
| ATTGATTTGGCTGTTGGGTT+AGG | 0.379250 | 8.4:+24214719 | MS.gene46359:CDS |
| TATATATTTGACTCTTTAGC+TGG | 0.419976 | 8.4:+24214749 | MS.gene46359:CDS |
| ACACTATTGATTTGGCTGTT+GGG | 0.428713 | 8.4:+24214714 | MS.gene46359:CDS |
| CTCGTGTGGACTTTCCAGTT+GGG | 0.452431 | 8.4:+24214537 | MS.gene46359:CDS |
| ACATGAGGAGGATGAAGAGA+TGG | 0.456825 | 8.4:+24214621 | MS.gene46359:CDS |
| CATGAGGAGGATGAAGAGAT+GGG | 0.476047 | 8.4:+24214622 | MS.gene46359:CDS |
| AATGAATCACCTGAGGAAAT+GGG | 0.485882 | 8.4:-24213901 | None:intergenic |
| CACACAGCTCCCATTTCCTC+AGG | 0.489274 | 8.4:+24213892 | MS.gene46359:CDS |
| GGGTTCGGTGATAGCTTCAT+TGG | 0.496149 | 8.4:+24214598 | MS.gene46359:CDS |
| TCTCGTGTGGACTTTCCAGT+TGG | 0.496967 | 8.4:+24214536 | MS.gene46359:CDS |
| TTGCAAGCTGTGAGATTGCT+TGG | 0.504819 | 8.4:+24214677 | MS.gene46359:CDS |
| GAGGATGAAGAGATGGGAGA+TGG | 0.513956 | 8.4:+24214628 | MS.gene46359:CDS |
| TGAAGAGATGGGAGATGGCA+TGG | 0.534871 | 8.4:+24214633 | MS.gene46359:CDS |
| GTGAGATTGCTTGGTACAAG+AGG | 0.580563 | 8.4:+24214686 | MS.gene46359:CDS |
| TGATAGCTTCATTGGACATG+AGG | 0.619875 | 8.4:+24214606 | MS.gene46359:CDS |
| TAGCTTCATTGGACATGAGG+AGG | 0.656474 | 8.4:+24214609 | MS.gene46359:CDS |
| CTGAGGAAATGGGAGCTGTG+TGG | 0.711136 | 8.4:-24213891 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGAATTCAATTAACATT+GGG | + | chr8.4:24214387-24214406 | MS.gene46359:intron | 15.0% |
| !! | ATTAACAAAACAAAAACACA+AGG | - | chr8.4:24214495-24214514 | None:intergenic | 20.0% |
| !! | GATTTGAATTCAATTAACAT+TGG | + | chr8.4:24214386-24214405 | MS.gene46359:intron | 20.0% |
| !! | TTTGAATTCAATTAACATTG+GGG | + | chr8.4:24214388-24214407 | MS.gene46359:intron | 20.0% |
| !!! | AAAAAATGAAGCATTTTGTT+TGG | - | chr8.4:24213957-24213976 | None:intergenic | 20.0% |
| !!! | ATAGAAGTGATTCTAAAAAA+TGG | + | chr8.4:24214105-24214124 | MS.gene46359:intron | 20.0% |
| !!! | TGTTTTGTTAATGTTGAATA+GGG | + | chr8.4:24214503-24214522 | MS.gene46359:intron | 20.0% |
| !!! | TTGTTTTGTTAATGTTGAAT+AGG | + | chr8.4:24214502-24214521 | MS.gene46359:intron | 20.0% |
| ! | CACGTTAAAAATCACATTTA+GGG | - | chr8.4:24214299-24214318 | None:intergenic | 25.0% |
| ! | TATATATTTGACTCTTTAGC+TGG | + | chr8.4:24214749-24214768 | MS.gene46359:CDS | 25.0% |
| ! | TCACGTTAAAAATCACATTT+AGG | - | chr8.4:24214300-24214319 | None:intergenic | 25.0% |
| ! | TTAAGATTGCAAGATTAGAT+TGG | + | chr8.4:24214427-24214446 | MS.gene46359:intron | 25.0% |
| ! | TTTAACATGAAATCGAAGTT+TGG | + | chr8.4:24214265-24214284 | MS.gene46359:intron | 25.0% |
| !! | AATGTTGAATAGGGATTTTT+GGG | + | chr8.4:24214512-24214531 | MS.gene46359:intron | 25.0% |
| !! | GAATTCAAACATAGTTCTTT+TGG | - | chr8.4:24214162-24214181 | None:intergenic | 25.0% |
| !! | TAATGTTGAATAGGGATTTT+TGG | + | chr8.4:24214511-24214530 | MS.gene46359:intron | 25.0% |
| !!! | AAACATGCATTTAGTTCTAT+TGG | - | chr8.4:24214066-24214085 | None:intergenic | 25.0% |
| !!! | ATCATTTTTCCTGTGTTTTT+GGG | + | chr8.4:24214565-24214584 | MS.gene46359:CDS | 25.0% |
| !!! | TATCATTTTTCCTGTGTTTT+TGG | + | chr8.4:24214564-24214583 | MS.gene46359:CDS | 25.0% |
| AAAAATGATACATTCCCAAC+TGG | - | chr8.4:24214554-24214573 | None:intergenic | 30.0% | |
| ACTAGAAGCTTACAATGAAA+GGG | + | chr8.4:24214211-24214230 | MS.gene46359:intron | 30.0% | |
| CATTGTAAGCTTCTAGTAAT+GGG | - | chr8.4:24214208-24214227 | None:intergenic | 30.0% | |
| TACTAGAAGCTTACAATGAA+AGG | + | chr8.4:24214210-24214229 | MS.gene46359:intron | 30.0% | |
| TCATTGTAAGCTTCTAGTAA+TGG | - | chr8.4:24214209-24214228 | None:intergenic | 30.0% | |
| ! | AGGCTATTACACTATTGATT+TGG | + | chr8.4:24214706-24214725 | MS.gene46359:CDS | 30.0% |
| ! | CAAACATAGTTCTTTTGGAT+TGG | - | chr8.4:24214157-24214176 | None:intergenic | 30.0% |
| !! | TCATTTTTCCTGTGTTTTTG+GGG | + | chr8.4:24214566-24214585 | MS.gene46359:CDS | 30.0% |
| !! | TGCATTTAGTTCTATTGGAT+TGG | - | chr8.4:24214061-24214080 | None:intergenic | 30.0% |
| AATGAATCACCTGAGGAAAT+GGG | - | chr8.4:24213904-24213923 | None:intergenic | 35.0% | |
| ACAATGAAAGGGATTCACTT+CGG | + | chr8.4:24214222-24214241 | MS.gene46359:intron | 35.0% | |
| GTATCTGTCTCTCAAATTAG+TGG | - | chr8.4:24214350-24214369 | None:intergenic | 35.0% | |
| TAACCTCCAAAATTGCTCTT+AGG | - | chr8.4:24214032-24214051 | None:intergenic | 35.0% | |
| TGAAATGAATGAATCACCTG+AGG | - | chr8.4:24213911-24213930 | None:intergenic | 35.0% | |
| ! | ATTCGTCCTAAGAGCAATTT+TGG | + | chr8.4:24214023-24214042 | MS.gene46359:intron | 35.0% |
| ! | TTACATGTCGTGGGATTTTT+GGG | + | chr8.4:24213864-24213883 | MS.gene46359:CDS | 35.0% |
| ! | TTTACATGTCGTGGGATTTT+TGG | + | chr8.4:24213863-24213882 | MS.gene46359:CDS | 35.0% |
| !! | ACACTATTGATTTGGCTGTT+GGG | + | chr8.4:24214714-24214733 | MS.gene46359:CDS | 35.0% |
| !! | TACACTATTGATTTGGCTGT+TGG | + | chr8.4:24214713-24214732 | MS.gene46359:CDS | 35.0% |
| GAATGAATCACCTGAGGAAA+TGG | - | chr8.4:24213905-24213924 | None:intergenic | 40.0% | |
| GTCTCTCAAATTAGTGGCAT+AGG | - | chr8.4:24214344-24214363 | None:intergenic | 40.0% | |
| TGATAGCTTCATTGGACATG+AGG | + | chr8.4:24214606-24214625 | MS.gene46359:CDS | 40.0% | |
| !! | ATTGATTTGGCTGTTGGGTT+AGG | + | chr8.4:24214719-24214738 | MS.gene46359:CDS | 40.0% |
| !!! | TTTTGGATTGGTCAGTGTGT+GGG | - | chr8.4:24214145-24214164 | None:intergenic | 40.0% |
| ACATGAGGAGGATGAAGAGA+TGG | + | chr8.4:24214621-24214640 | MS.gene46359:CDS | 45.0% | |
| CATGAGGAGGATGAAGAGAT+GGG | + | chr8.4:24214622-24214641 | MS.gene46359:CDS | 45.0% | |
| GTGAGATTGCTTGGTACAAG+AGG | + | chr8.4:24214686-24214705 | MS.gene46359:CDS | 45.0% | |
| TAGCTTCATTGGACATGAGG+AGG | + | chr8.4:24214609-24214628 | MS.gene46359:CDS | 45.0% | |
| TTGCAAGCTGTGAGATTGCT+TGG | + | chr8.4:24214677-24214696 | MS.gene46359:CDS | 45.0% | |
| ! | CGTCCTAAGAGCAATTTTGG+AGG | + | chr8.4:24214026-24214045 | MS.gene46359:intron | 45.0% |
| !!! | CTTTTGGATTGGTCAGTGTG+TGG | - | chr8.4:24214146-24214165 | None:intergenic | 45.0% |
| CCACATGACCCCAAAAACAC+AGG | - | chr8.4:24214577-24214596 | None:intergenic | 50.0% | |
| CTCGTGTGGACTTTCCAGTT+GGG | + | chr8.4:24214537-24214556 | MS.gene46359:CDS | 50.0% | |
| GAGGATGAAGAGATGGGAGA+TGG | + | chr8.4:24214628-24214647 | MS.gene46359:CDS | 50.0% | |
| TCTCGTGTGGACTTTCCAGT+TGG | + | chr8.4:24214536-24214555 | MS.gene46359:CDS | 50.0% | |
| TGAAGAGATGGGAGATGGCA+TGG | + | chr8.4:24214633-24214652 | MS.gene46359:CDS | 50.0% | |
| !! | GGGTTCGGTGATAGCTTCAT+TGG | + | chr8.4:24214598-24214617 | MS.gene46359:CDS | 50.0% |
| !!! | CCTGTGTTTTTGGGGTCATG+TGG | + | chr8.4:24214574-24214593 | MS.gene46359:CDS | 50.0% |
| !!! | GGGATTTTTGGGTTCTCGTG+TGG | + | chr8.4:24214523-24214542 | MS.gene46359:intron | 50.0% |
| !!! | GTGTTTTTGGGGTCATGTGG+CGG | + | chr8.4:24214577-24214596 | MS.gene46359:CDS | 50.0% |
| !!! | TGTTTTTGGGGTCATGTGGC+GGG | + | chr8.4:24214578-24214597 | MS.gene46359:CDS | 50.0% |
| CACACAGCTCCCATTTCCTC+AGG | + | chr8.4:24213892-24213911 | MS.gene46359:CDS | 55.0% | |
| CTGAGGAAATGGGAGCTGTG+TGG | - | chr8.4:24213894-24213913 | None:intergenic | 55.0% | |
| !! | TTGGGGTCATGTGGCGGGTT+CGG | + | chr8.4:24214583-24214602 | MS.gene46359:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 24213860 | 24214814 | 24213860 | ID=MS.gene46359 |
| chr8.4 | mRNA | 24213860 | 24214814 | 24213860 | ID=MS.gene46359.t1;Parent=MS.gene46359 |
| chr8.4 | exon | 24213860 | 24213913 | 24213860 | ID=MS.gene46359.t1.exon1;Parent=MS.gene46359.t1 |
| chr8.4 | CDS | 24213860 | 24213913 | 24213860 | ID=cds.MS.gene46359.t1;Parent=MS.gene46359.t1 |
| chr8.4 | exon | 24214524 | 24214814 | 24214524 | ID=MS.gene46359.t1.exon2;Parent=MS.gene46359.t1 |
| chr8.4 | CDS | 24214524 | 24214814 | 24214524 | ID=cds.MS.gene46359.t1;Parent=MS.gene46359.t1 |
| Gene Sequence |
| Protein sequence |
