Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050611 | MS.gene47167 | 0.838833 | 2.27E-57 | -1.69E-46 |
| MS.gene051181 | MS.gene47167 | 0.802133 | 6.48E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene47167 | 0.831352 | 1.75E-55 | -1.69E-46 |
| MS.gene055058 | MS.gene47167 | 0.828127 | 1.07E-54 | -1.69E-46 |
| MS.gene058294 | MS.gene47167 | 0.812663 | 3.79E-51 | -1.69E-46 |
| MS.gene058515 | MS.gene47167 | 0.833229 | 6.01E-56 | -1.69E-46 |
| MS.gene059276 | MS.gene47167 | 0.816214 | 6.21E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47167.t1 | MTR_8g432580 | 85.135 | 74 | 11 | 0 | 6 | 79 | 52 | 125 | 1.75e-22 | 87.0 |
| MS.gene47167.t1 | MTR_4g074930 | 86.486 | 74 | 9 | 1 | 2 | 75 | 28 | 100 | 2.29e-22 | 91.7 |
| MS.gene47167.t1 | MTR_4g074930 | 86.486 | 74 | 9 | 1 | 2 | 75 | 28 | 100 | 2.39e-22 | 91.7 |
| MS.gene47167.t1 | MTR_4g074930 | 86.486 | 74 | 9 | 1 | 2 | 75 | 28 | 100 | 2.42e-22 | 91.7 |
| MS.gene47167.t1 | MTR_4g074930 | 86.486 | 74 | 9 | 1 | 2 | 75 | 28 | 100 | 2.76e-22 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGAAGGAGGAAAGTAGTT+TGG | 0.335928 | 6.3:+17130503 | MS.gene47167:CDS |
| TCCCACATCGTACTATTATC+AGG | 0.413728 | 6.3:+17131387 | MS.gene47167:CDS |
| CACTTCTTCCTCATCGGAAG+AGG | 0.423585 | 6.3:-17130785 | None:intergenic |
| CTCTGAAGAGGTCTCTGGCT+GGG | 0.424395 | 6.3:-17130740 | None:intergenic |
| CCTCTGAAGAGGTCTCTGGC+TGG | 0.431390 | 6.3:-17130741 | None:intergenic |
| CGCCAAGCAAAAGGCGTTGA+AGG | 0.441849 | 6.3:+17130476 | MS.gene47167:CDS |
| GATTCCTCTGAAGAGGTCTC+TGG | 0.466816 | 6.3:-17130745 | None:intergenic |
| CACTTTCACTTCTTCCTCAT+CGG | 0.467568 | 6.3:-17130791 | None:intergenic |
| AGAGGTTGCCGCCAAGCAAA+AGG | 0.479123 | 6.3:+17130467 | MS.gene47167:CDS |
| AATGAAATTAAAACCATGGC+AGG | 0.509360 | 6.3:+17131355 | MS.gene47167:CDS |
| CTCTGAGGAATCTGAGGATG+AGG | 0.511350 | 6.3:+17130533 | MS.gene47167:CDS |
| GAAGGAGGAAAGTAGTTTGG+AGG | 0.514144 | 6.3:+17130506 | MS.gene47167:CDS |
| GGCGTTGAAGGTAGTCAAGA+AGG | 0.528027 | 6.3:+17130488 | MS.gene47167:CDS |
| GTGGGTACTAGAGAATTCAT+AGG | 0.534544 | 6.3:+17131413 | MS.gene47167:CDS |
| CAGTGAGTGCAAAGAAGCAA+GGG | 0.541082 | 6.3:+17130439 | MS.gene47167:CDS |
| CTTTGATATAGGCAAGAGGC+AGG | 0.543584 | 6.3:+17130398 | MS.gene47167:intron |
| GCAGGCTGAGGAAGAAGTCA+AGG | 0.547712 | 6.3:+17130416 | MS.gene47167:CDS |
| AGAGTCAGATTCCTCTGAAG+AGG | 0.552798 | 6.3:-17130752 | None:intergenic |
| GCAGTGAGTGCAAAGAAGCA+AGG | 0.558038 | 6.3:+17130438 | MS.gene47167:CDS |
| CATATGTAGACACTGTCATA+GGG | 0.583382 | 6.3:-17131444 | None:intergenic |
| GGAATCCTCTGAGGAATCTG+AGG | 0.584932 | 6.3:+17130527 | MS.gene47167:CDS |
| GTCAAATGAAATTAAAACCA+TGG | 0.588337 | 6.3:+17131351 | MS.gene47167:CDS |
| TATAGGCAAGAGGCAGGCTG+AGG | 0.588543 | 6.3:+17130404 | MS.gene47167:intron |
| TCGTACTATTATCAGGCAAG+TGG | 0.597519 | 6.3:+17131394 | MS.gene47167:CDS |
| AAAGAAGCAAGGGGTAGAAG+AGG | 0.598623 | 6.3:+17130449 | MS.gene47167:CDS |
| TGCCTGATAATAGTACGATG+TGG | 0.623695 | 6.3:-17131389 | None:intergenic |
| TTCACTTTGATATAGGCAAG+AGG | 0.629817 | 6.3:+17130394 | MS.gene47167:intron |
| CGTACTATTATCAGGCAAGT+GGG | 0.636740 | 6.3:+17131395 | MS.gene47167:CDS |
| CCAGCCAGAGACCTCTTCAG+AGG | 0.641154 | 6.3:+17130741 | MS.gene47167:CDS |
| GCCTGATAATAGTACGATGT+GGG | 0.647015 | 6.3:-17131388 | None:intergenic |
| TGACAGCTCCTCTTCCGATG+AGG | 0.667005 | 6.3:+17130777 | MS.gene47167:CDS |
| AGTGAGTGCAAAGAAGCAAG+GGG | 0.679652 | 6.3:+17130440 | MS.gene47167:CDS |
| GTTGAAGGTAGTCAAGAAGG+AGG | 0.683060 | 6.3:+17130491 | MS.gene47167:CDS |
| CTCATCCTCAGATTCCTCAG+AGG | 0.686828 | 6.3:-17130532 | None:intergenic |
| TAGTTTGGAGGAATCCTCTG+AGG | 0.723183 | 6.3:+17130518 | MS.gene47167:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAATATATTTTTGAAACT+TGG | + | chr6.3:17129915-17129934 | MS.gene47167:intron | 10.0% |
| !!! | ATGTTTAAGATATAAATCAA+AGG | - | chr6.3:17130864-17130883 | None:intergenic | 15.0% |
| !! | AGAACATCAAATAATATTGA+AGG | - | chr6.3:17130254-17130273 | None:intergenic | 20.0% |
| !! | ATAATAACATCAGACAAAAT+TGG | - | chr6.3:17130164-17130183 | None:intergenic | 20.0% |
| !! | TAATAACATCAGACAAAATT+GGG | - | chr6.3:17130163-17130182 | None:intergenic | 20.0% |
| !!! | ATTTTTTTATGTTCTGATGT+CGG | + | chr6.3:17130353-17130372 | MS.gene47167:intron | 20.0% |
| !!! | GTTTTAAAAAACAGAACAAT+AGG | - | chr6.3:17130955-17130974 | None:intergenic | 20.0% |
| !!! | TCTATAATTCACTTTTTAGA+AGG | + | chr6.3:17130978-17130997 | MS.gene47167:intron | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | - | chr6.3:17131255-17131274 | None:intergenic | 20.0% |
| !!! | TTCTAAAAAGTGAATTATAG+AGG | - | chr6.3:17130979-17130998 | None:intergenic | 20.0% |
| !!! | TTTTCTGATGTTGATTATAT+AGG | + | chr6.3:17130190-17130209 | MS.gene47167:intron | 20.0% |
| ! | AATAACATCAGACAAAATTG+GGG | - | chr6.3:17130162-17130181 | None:intergenic | 25.0% |
| ! | ATGTTAGTATAGATGTTGAT+TGG | + | chr6.3:17130099-17130118 | MS.gene47167:intron | 25.0% |
| ! | GTCAAATGAAATTAAAACCA+TGG | + | chr6.3:17131351-17131370 | MS.gene47167:CDS | 25.0% |
| ! | TTATATCTTAAACACTGTGT+TGG | + | chr6.3:17130926-17130945 | MS.gene47167:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | - | chr6.3:17131196-17131215 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | - | chr6.3:17131195-17131214 | None:intergenic | 25.0% |
| !! | GTGTTTTATGTTATGAAGTT+AGG | + | chr6.3:17130052-17130071 | MS.gene47167:intron | 25.0% |
| !! | TATGTCATGTTAATTTTACG+TGG | + | chr6.3:17130644-17130663 | MS.gene47167:intron | 25.0% |
| !!! | CTTCTAGATTTTGGTTAATT+CGG | - | chr6.3:17130222-17130241 | None:intergenic | 25.0% |
| AAATCGTATAAATGTCTCGA+TGG | - | chr6.3:17130028-17130047 | None:intergenic | 30.0% | |
| AATGAAATTAAAACCATGGC+AGG | + | chr6.3:17131355-17131374 | MS.gene47167:CDS | 30.0% | |
| ATAACATCAGACAAAATTGG+GGG | - | chr6.3:17130161-17130180 | None:intergenic | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | + | chr6.3:17131192-17131211 | MS.gene47167:intron | 30.0% | |
| GAGACATTTATACGATTTAC+TGG | + | chr6.3:17130030-17130049 | MS.gene47167:intron | 30.0% | |
| TAGTTATCAGCAAATGAGAT+AGG | + | chr6.3:17131167-17131186 | MS.gene47167:intron | 30.0% | |
| TTAACCAAAATCTAGAAGCT+TGG | + | chr6.3:17130224-17130243 | MS.gene47167:intron | 30.0% | |
| ! | AGAATAGGAGAAAGGATTTT+TGG | - | chr6.3:17131071-17131090 | None:intergenic | 30.0% |
| ! | CATTTGCTGATAACTACAAA+AGG | - | chr6.3:17131163-17131182 | None:intergenic | 30.0% |
| ! | GAATAGGAGAAAGGATTTTT+GGG | - | chr6.3:17131070-17131089 | None:intergenic | 30.0% |
| !! | GGCTATTTTCACTTTGATAT+AGG | + | chr6.3:17130387-17130406 | MS.gene47167:intron | 30.0% |
| !! | TTTTTCAGTTTCTTCCAGTT+AGG | - | chr6.3:17131133-17131152 | None:intergenic | 30.0% |
| !!! | TCACATTCCCATTTTTAGTT+GGG | - | chr6.3:17130714-17130733 | None:intergenic | 30.0% |
| !!! | TTCACATTCCCATTTTTAGT+TGG | - | chr6.3:17130715-17130734 | None:intergenic | 30.0% |
| AAGAAGACCCCAACTAAAAA+TGG | + | chr6.3:17130703-17130722 | MS.gene47167:CDS | 35.0% | |
| AAGTGTGAGCAACAAAATAG+TGG | - | chr6.3:17131018-17131037 | None:intergenic | 35.0% | |
| AGAAGACCCCAACTAAAAAT+GGG | + | chr6.3:17130704-17130723 | MS.gene47167:CDS | 35.0% | |
| ATAGGTCAAGAATAGGAGAA+AGG | - | chr6.3:17131079-17131098 | None:intergenic | 35.0% | |
| ATGCTTTAACGCATTTCGAA+TGG | + | chr6.3:17130574-17130593 | MS.gene47167:intron | 35.0% | |
| TTCACTTTGATATAGGCAAG+AGG | + | chr6.3:17130394-17130413 | MS.gene47167:intron | 35.0% | |
| TTTCTCCTATTCTTGACCTA+TGG | + | chr6.3:17131078-17131097 | MS.gene47167:intron | 35.0% | |
| ! | AGAACCAAGCTTCTAGATTT+TGG | - | chr6.3:17130231-17130250 | None:intergenic | 35.0% |
| ! | GATTGGTCAAACTGATTTCT+CGG | + | chr6.3:17130116-17130135 | MS.gene47167:intron | 35.0% |
| !! | GCTGCTTACTTTTGATATTG+TGG | + | chr6.3:17131281-17131300 | MS.gene47167:intron | 35.0% |
| !!! | CACATTCCCATTTTTAGTTG+GGG | - | chr6.3:17130713-17130732 | None:intergenic | 35.0% |
| !!! | CTGATGTCGGTTTTCTTATT+GGG | + | chr6.3:17130366-17130385 | MS.gene47167:intron | 35.0% |
| !!! | TCTGATGTCGGTTTTCTTAT+TGG | + | chr6.3:17130365-17130384 | MS.gene47167:intron | 35.0% |
| !!! | TTTTGGGTTGGAAACAAAAC+AGG | - | chr6.3:17131054-17131073 | None:intergenic | 35.0% |
| CAAGAAGGAGGAAAGTAGTT+TGG | + | chr6.3:17130503-17130522 | MS.gene47167:CDS | 40.0% | |
| CACTTTCACTTCTTCCTCAT+CGG | - | chr6.3:17130794-17130813 | None:intergenic | 40.0% | |
| CGTACTATTATCAGGCAAGT+GGG | + | chr6.3:17131395-17131414 | MS.gene47167:CDS | 40.0% | |
| GAGATAGGATCCCTATTATG+AGG | + | chr6.3:17131182-17131201 | MS.gene47167:intron | 40.0% | |
| GCCTGATAATAGTACGATGT+GGG | - | chr6.3:17131391-17131410 | None:intergenic | 40.0% | |
| GTGGGTACTAGAGAATTCAT+AGG | + | chr6.3:17131413-17131432 | MS.gene47167:CDS | 40.0% | |
| TCCCACATCGTACTATTATC+AGG | + | chr6.3:17131387-17131406 | MS.gene47167:CDS | 40.0% | |
| TCGTACTATTATCAGGCAAG+TGG | + | chr6.3:17131394-17131413 | MS.gene47167:CDS | 40.0% | |
| TGCCTGATAATAGTACGATG+TGG | - | chr6.3:17131392-17131411 | None:intergenic | 40.0% | |
| TTTCTCGGAAAGATGAAGCT+TGG | + | chr6.3:17130131-17130150 | MS.gene47167:intron | 40.0% | |
| !!! | AGGAGAAAGGATTTTTGGGT+TGG | - | chr6.3:17131066-17131085 | None:intergenic | 40.0% |
| AAAGAAGCAAGGGGTAGAAG+AGG | + | chr6.3:17130449-17130468 | MS.gene47167:CDS | 45.0% | |
| AGTGAGTGCAAAGAAGCAAG+GGG | + | chr6.3:17130440-17130459 | MS.gene47167:CDS | 45.0% | |
| CAGTGAGTGCAAAGAAGCAA+GGG | + | chr6.3:17130439-17130458 | MS.gene47167:CDS | 45.0% | |
| CTTTGATATAGGCAAGAGGC+AGG | + | chr6.3:17130398-17130417 | MS.gene47167:intron | 45.0% | |
| GAAGGAGGAAAGTAGTTTGG+AGG | + | chr6.3:17130506-17130525 | MS.gene47167:CDS | 45.0% | |
| GTTGAAGGTAGTCAAGAAGG+AGG | + | chr6.3:17130491-17130510 | MS.gene47167:CDS | 45.0% | |
| TAGTTTGGAGGAATCCTCTG+AGG | + | chr6.3:17130518-17130537 | MS.gene47167:CDS | 45.0% | |
| TCCAGCCATAGGTCAAGAAT+AGG | - | chr6.3:17131086-17131105 | None:intergenic | 45.0% | |
| TCCTATTCTTGACCTATGGC+TGG | + | chr6.3:17131082-17131101 | MS.gene47167:intron | 45.0% | |
| TCTTCCAGTTAGGCAGTGAT+AGG | - | chr6.3:17131123-17131142 | None:intergenic | 45.0% | |
| ! | AAAGCAGGAGCTTTTGCAAC+AGG | - | chr6.3:17130679-17130698 | None:intergenic | 45.0% |
| ! | AGAGTCAGATTCCTCTGAAG+AGG | - | chr6.3:17130755-17130774 | None:intergenic | 45.0% |
| ! | GGGGTCTTCTTTGAAAAAGC+AGG | - | chr6.3:17130694-17130713 | None:intergenic | 45.0% |
| ! | TACCTTCAACGCCTTTTGCT+TGG | - | chr6.3:17130481-17130500 | None:intergenic | 45.0% |
| CACTTCTTCCTCATCGGAAG+AGG | - | chr6.3:17130788-17130807 | None:intergenic | 50.0% | |
| CAGTTAGGCAGTGATAGGAG+AGG | - | chr6.3:17131118-17131137 | None:intergenic | 50.0% | |
| CTCATCCTCAGATTCCTCAG+AGG | - | chr6.3:17130535-17130554 | None:intergenic | 50.0% | |
| CTCTCCTATCACTGCCTAAC+TGG | + | chr6.3:17131116-17131135 | MS.gene47167:intron | 50.0% | |
| GCAGTGAGTGCAAAGAAGCA+AGG | + | chr6.3:17130438-17130457 | MS.gene47167:CDS | 50.0% | |
| GGAGAGTTTCTTCCAGCCAT+AGG | - | chr6.3:17131097-17131116 | None:intergenic | 50.0% | |
| ! | CTCTGAGGAATCTGAGGATG+AGG | + | chr6.3:17130533-17130552 | MS.gene47167:CDS | 50.0% |
| ! | CTTCAACGCCTTTTGCTTGG+CGG | - | chr6.3:17130478-17130497 | None:intergenic | 50.0% |
| ! | GATTCCTCTGAAGAGGTCTC+TGG | - | chr6.3:17130748-17130767 | None:intergenic | 50.0% |
| ! | GGAATCCTCTGAGGAATCTG+AGG | + | chr6.3:17130527-17130546 | MS.gene47167:CDS | 50.0% |
| ! | GGCGTTGAAGGTAGTCAAGA+AGG | + | chr6.3:17130488-17130507 | MS.gene47167:CDS | 50.0% |
| AGAGGTTGCCGCCAAGCAAA+AGG | + | chr6.3:17130467-17130486 | MS.gene47167:CDS | 55.0% | |
| GCAGGCTGAGGAAGAAGTCA+AGG | + | chr6.3:17130416-17130435 | MS.gene47167:CDS | 55.0% | |
| TATAGGCAAGAGGCAGGCTG+AGG | + | chr6.3:17130404-17130423 | MS.gene47167:intron | 55.0% | |
| TGACAGCTCCTCTTCCGATG+AGG | + | chr6.3:17130777-17130796 | MS.gene47167:CDS | 55.0% | |
| ! | CTCTGAAGAGGTCTCTGGCT+GGG | - | chr6.3:17130743-17130762 | None:intergenic | 55.0% |
| ! | GGGATGTTTTGTGCCTGCCA+TGG | - | chr6.3:17131371-17131390 | None:intergenic | 55.0% |
| !! | CGCCAAGCAAAAGGCGTTGA+AGG | + | chr6.3:17130476-17130495 | MS.gene47167:CDS | 55.0% |
| CCAGCCAGAGACCTCTTCAG+AGG | + | chr6.3:17130741-17130760 | MS.gene47167:CDS | 60.0% | |
| ! | CCTCTGAAGAGGTCTCTGGC+TGG | - | chr6.3:17130744-17130763 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 17129837 | 17131451 | 17129837 | ID=MS.gene47167 |
| chr6.3 | mRNA | 17129837 | 17131451 | 17129837 | ID=MS.gene47167.t1;Parent=MS.gene47167 |
| chr6.3 | exon | 17129837 | 17129840 | 17129837 | ID=MS.gene47167.t1.exon1;Parent=MS.gene47167.t1 |
| chr6.3 | CDS | 17129837 | 17129840 | 17129837 | ID=cds.MS.gene47167.t1;Parent=MS.gene47167.t1 |
| chr6.3 | exon | 17130409 | 17130554 | 17130409 | ID=MS.gene47167.t1.exon2;Parent=MS.gene47167.t1 |
| chr6.3 | CDS | 17130409 | 17130554 | 17130409 | ID=cds.MS.gene47167.t1;Parent=MS.gene47167.t1 |
| chr6.3 | exon | 17130673 | 17130810 | 17130673 | ID=MS.gene47167.t1.exon3;Parent=MS.gene47167.t1 |
| chr6.3 | CDS | 17130673 | 17130810 | 17130673 | ID=cds.MS.gene47167.t1;Parent=MS.gene47167.t1 |
| chr6.3 | exon | 17131338 | 17131451 | 17131338 | ID=MS.gene47167.t1.exon4;Parent=MS.gene47167.t1 |
| chr6.3 | CDS | 17131338 | 17131451 | 17131338 | ID=cds.MS.gene47167.t1;Parent=MS.gene47167.t1 |
| Gene Sequence |
| Protein sequence |
