AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48068


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48068.t1 MTR_5g094340 93.765 417 25 1 1 416 1 417 0.0 769
MS.gene48068.t1 MTR_3g055960 73.210 433 96 8 1 416 3 432 0.0 595
MS.gene48068.t1 MTR_5g089720 39.663 416 189 10 53 416 32 437 3.22e-86 269
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48068.t1 AT5G55530 55.607 428 155 12 1 416 1 405 1.91e-145 420
MS.gene48068.t1 AT5G55530 55.607 428 155 12 1 416 1 405 1.91e-145 420
MS.gene48068.t1 AT5G55530 55.607 428 155 12 1 416 1 405 1.91e-145 420
MS.gene48068.t1 AT1G50570 55.039 387 145 10 39 416 22 388 1.27e-127 374
MS.gene48068.t1 AT1G50570 55.039 387 145 10 39 416 22 388 1.27e-127 374
MS.gene48068.t1 AT5G12300 47.500 200 96 4 54 247 19 215 3.03e-53 182

Find 71 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAATGAAGACCACTTAA+TGG 0.202475 5.3:+76785930 MS.gene48068:CDS
TCCAATCAGGAGCAAATTTC+AGG 0.224053 5.3:+76786159 MS.gene48068:CDS
TTCACACTCACAACAGGTTT+TGG 0.272566 5.3:-76786255 None:intergenic
TAGCATAAACATCTTGCTTA+TGG 0.294667 5.3:-76785462 None:intergenic
TCGAATCCAGAGGAATTTAT+TGG 0.302487 5.3:+76785385 MS.gene48068:CDS
GAAAATCTTAGTTCAGAAAA+AGG 0.307362 5.3:+76785997 MS.gene48068:CDS
CCAAAGCAAAACCAAGTAAT+TGG 0.309892 5.3:-76785654 None:intergenic
TTTCTGTCCAATTCAGTATT+AGG 0.324501 5.3:-76785829 None:intergenic
ATATCCTGCTGCACCATCTT+TGG 0.325154 5.3:-76786291 None:intergenic
TCCTCCTCCATTGATAATCT+TGG 0.335282 5.3:-76785524 None:intergenic
GAAAGTGGACCAACTAGTTC+AGG 0.360152 5.3:+76786387 MS.gene48068:CDS
ACCTGAAATTTGCTCCTGAT+TGG 0.370968 5.3:-76786160 None:intergenic
AGCAATGCCTAATACTGAAT+TGG 0.385474 5.3:+76785822 MS.gene48068:CDS
TGCTGCACCATCTTTGGCTC+TGG 0.397036 5.3:-76786285 None:intergenic
ATAACCTTCGTCTAAGTGTT+AGG 0.404097 5.3:+76785566 MS.gene48068:CDS
GTCAATACCAAGATTATCAA+TGG 0.409373 5.3:+76785517 MS.gene48068:CDS
TCTAAAGACATTGAGGTCAA+TGG 0.413522 5.3:+76785343 MS.gene48068:CDS
TGGCGCTTCACCAGATGTTA+TGG 0.425510 5.3:+76785792 MS.gene48068:CDS
TATCTTGAAGACCAATTACT+TGG 0.432499 5.3:+76785643 MS.gene48068:CDS
TCAATATTCACACTCACAAC+AGG 0.440373 5.3:-76786261 None:intergenic
GATGGTGCAGCAGGATATAG+TGG 0.440448 5.3:+76786296 MS.gene48068:CDS
GTGAGATCTGGATGCTTAGC+AGG 0.442573 5.3:+76785611 MS.gene48068:CDS
CTTCTCTCAAATGTGAGATC+TGG 0.446006 5.3:+76785599 MS.gene48068:CDS
ACCATTCAAGAGTTCTGTCT+TGG 0.461139 5.3:+76785267 MS.gene48068:CDS
TGCTACTAAATCTTCCAATC+AGG 0.468664 5.3:+76786146 MS.gene48068:CDS
TCTGAAGTATTGATGAAAGA+TGG 0.470375 5.3:+76785685 MS.gene48068:CDS
CTCATTCAGCAACGTATTCG+AGG 0.473738 5.3:-76786226 None:intergenic
TGAATCTGTTCAACCTCCTA+AGG 0.483694 5.3:+76786056 MS.gene48068:CDS
GAACAAAACCTGCTGGTGAA+TGG 0.486115 5.3:-76785750 None:intergenic
CTAGGTGGAGATGCAACCTT+AGG 0.492862 5.3:-76786072 None:intergenic
ATACTTTGGTACTGATAGCT+TGG 0.493813 5.3:+76785960 MS.gene48068:CDS
AGAGCCAAAGATGGTGCAGC+AGG 0.494831 5.3:+76786287 MS.gene48068:CDS
ATGTTTACATACATCAAGCT+AGG 0.499383 5.3:+76785416 MS.gene48068:CDS
CCATTCAAGAGTTCTGTCTT+GGG 0.499630 5.3:+76785268 MS.gene48068:CDS
GTTGAGTTTCCTGAACTAGT+TGG 0.500780 5.3:-76786396 None:intergenic
ACTACTCCAATAAATTCCTC+TGG 0.500999 5.3:-76785391 None:intergenic
GATCTATTCCATTCACCAGC+AGG 0.502121 5.3:+76785742 MS.gene48068:CDS
TCCTTTGTCTAAAGACATTG+AGG 0.512174 5.3:+76785336 MS.gene48068:CDS
TGGTGTTGTTTCCTCTTCAG+TGG 0.515128 5.3:+76786101 MS.gene48068:CDS
AAGAGTTCTGTCTTGGGACA+TGG 0.516859 5.3:+76785274 MS.gene48068:CDS
CTTCCTATGGACTTTGAAAG+TGG 0.518348 5.3:+76786372 MS.gene48068:CDS
GCATCTCCACCTAGCAGTAA+TGG 0.534582 5.3:+76786081 MS.gene48068:CDS
TTAATGGTTTCAGAATACTT+TGG 0.535977 5.3:+76785946 MS.gene48068:CDS
AGTATTCTGAAACCATTAAG+TGG 0.538501 5.3:-76785942 None:intergenic
GCTGAAATTGCCATAACATC+TGG 0.541367 5.3:-76785802 None:intergenic
GGTGGAGATGCAACCTTAGG+AGG 0.554345 5.3:-76786069 None:intergenic
ACCAAGATTATCAATGGAGG+AGG 0.555656 5.3:+76785523 MS.gene48068:CDS
AATACTGAATTGGACAGAAA+CGG 0.555790 5.3:+76785832 MS.gene48068:CDS
ACATTTGAGAGAAGAATCAA+TGG 0.559219 5.3:-76785590 None:intergenic
CCCAAGACAGAACTCTTGAA+TGG 0.559287 5.3:-76785268 None:intergenic
TGAGCTTGCAGCCACTGAAG+AGG 0.564334 5.3:-76786112 None:intergenic
CAATTGTCACTTGCTTACAA+TGG 0.566767 5.3:+76785772 MS.gene48068:CDS
AGATTGTAGTTTCTGTTCAG+AGG 0.567533 5.3:-76786418 None:intergenic
GGTCCACTTTCAAAGTCCAT+AGG 0.582924 5.3:-76786375 None:intergenic
AATGGTGACACAACAGATTG+TGG 0.593267 5.3:-76785250 None:intergenic
CGAAGGTTATCGTTAAACAC+GGG 0.594871 5.3:-76785553 None:intergenic
TTCATCAATACTTCAGACAA+AGG 0.596605 5.3:-76785679 None:intergenic
AAAAGAGTTGATCTCAAAGA+CGG 0.598346 5.3:+76786192 MS.gene48068:CDS
ACCTCAATGTCTTTAGACAA+AGG 0.602535 5.3:-76785337 None:intergenic
TATTGAGCCAGAGCCAAAGA+TGG 0.602654 5.3:+76786278 MS.gene48068:CDS
TGCTGGTGAATGGAATAGAT+CGG 0.604836 5.3:-76785740 None:intergenic
TGTTTACATACATCAAGCTA+GGG 0.605167 5.3:+76785417 MS.gene48068:CDS
GACAATTGAACAAAACCTGC+TGG 0.612847 5.3:-76785757 None:intergenic
GAAACAACACCATTACTGCT+AGG 0.628325 5.3:-76786090 None:intergenic
TGGTCCTAACACTTAGACGA+AGG 0.635127 5.3:-76785570 None:intergenic
AATACCAAGATTATCAATGG+AGG 0.656844 5.3:+76785520 MS.gene48068:CDS
ACGAAGGTTATCGTTAAACA+CGG 0.658274 5.3:-76785554 None:intergenic
TGTTGCAGTGTCGAATCCAG+AGG 0.689971 5.3:+76785375 MS.gene48068:CDS
ACAACACCATTACTGCTAGG+TGG 0.692805 5.3:-76786087 None:intergenic
TGAGATCTGGATGCTTAGCA+GGG 0.697112 5.3:+76785612 MS.gene48068:CDS
GAAGGTTATCGTTAAACACG+GGG 0.724753 5.3:-76785552 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTACAATTTTTGGATTT+GGG - chr5.3:76785918-76785937 None:intergenic 20.0%
!!! TCATTTACAATTTTTGGATT+TGG - chr5.3:76785919-76785938 None:intergenic 20.0%
! GAAAATCTTAGTTCAGAAAA+AGG + chr5.3:76785997-76786016 MS.gene48068:CDS 25.0%
! TTAATGGTTTCAGAATACTT+TGG + chr5.3:76785946-76785965 MS.gene48068:CDS 25.0%
!! TGGTCTTCATTTACAATTTT+TGG - chr5.3:76785925-76785944 None:intergenic 25.0%
!!! TTGGTATTGACAAAATTTTC+AGG - chr5.3:76785508-76785527 None:intergenic 25.0%
AATACCAAGATTATCAATGG+AGG + chr5.3:76785520-76785539 MS.gene48068:CDS 30.0%
AATACTGAATTGGACAGAAA+CGG + chr5.3:76785832-76785851 MS.gene48068:CDS 30.0%
ACATTTGAGAGAAGAATCAA+TGG - chr5.3:76785593-76785612 None:intergenic 30.0%
ATGTTTACATACATCAAGCT+AGG + chr5.3:76785416-76785435 MS.gene48068:CDS 30.0%
GTCAATACCAAGATTATCAA+TGG + chr5.3:76785517-76785536 MS.gene48068:CDS 30.0%
TAGCATAAACATCTTGCTTA+TGG - chr5.3:76785465-76785484 None:intergenic 30.0%
TATCTTGAAGACCAATTACT+TGG + chr5.3:76785643-76785662 MS.gene48068:CDS 30.0%
TGTAAATGAAGACCACTTAA+TGG + chr5.3:76785930-76785949 MS.gene48068:CDS 30.0%
TGTTTACATACATCAAGCTA+GGG + chr5.3:76785417-76785436 MS.gene48068:CDS 30.0%
TTCATCAATACTTCAGACAA+AGG - chr5.3:76785682-76785701 None:intergenic 30.0%
TTTCTGTCCAATTCAGTATT+AGG - chr5.3:76785832-76785851 None:intergenic 30.0%
! AAAAGAGTTGATCTCAAAGA+CGG + chr5.3:76786192-76786211 MS.gene48068:CDS 30.0%
! AAGATTTTCAGTATCAGTAG+TGG - chr5.3:76785986-76786005 None:intergenic 30.0%
!! AGTATTCTGAAACCATTAAG+TGG - chr5.3:76785945-76785964 None:intergenic 30.0%
!! CTTCATTTTTGCTAATGACT+CGG - chr5.3:76786352-76786371 None:intergenic 30.0%
!! TCTGAAGTATTGATGAAAGA+TGG + chr5.3:76785685-76785704 MS.gene48068:CDS 30.0%
ACCTCAATGTCTTTAGACAA+AGG - chr5.3:76785340-76785359 None:intergenic 35.0%
ACGAAGGTTATCGTTAAACA+CGG - chr5.3:76785557-76785576 None:intergenic 35.0%
ACTACTCCAATAAATTCCTC+TGG - chr5.3:76785394-76785413 None:intergenic 35.0%
AGATTGTAGTTTCTGTTCAG+AGG - chr5.3:76786421-76786440 None:intergenic 35.0%
AGCAAAAATGAAGCTTCCTA+TGG + chr5.3:76786359-76786378 MS.gene48068:CDS 35.0%
AGCAATGCCTAATACTGAAT+TGG + chr5.3:76785822-76785841 MS.gene48068:CDS 35.0%
ATAACCTTCGTCTAAGTGTT+AGG + chr5.3:76785566-76785585 MS.gene48068:CDS 35.0%
CCAAAGCAAAACCAAGTAAT+TGG - chr5.3:76785657-76785676 None:intergenic 35.0%
TCAATATTCACACTCACAAC+AGG - chr5.3:76786264-76786283 None:intergenic 35.0%
TCCTTTGTCTAAAGACATTG+AGG + chr5.3:76785336-76785355 MS.gene48068:CDS 35.0%
TCGAATCCAGAGGAATTTAT+TGG + chr5.3:76785385-76785404 MS.gene48068:CDS 35.0%
TCTAAAGACATTGAGGTCAA+TGG + chr5.3:76785343-76785362 MS.gene48068:CDS 35.0%
TGCTACTAAATCTTCCAATC+AGG + chr5.3:76786146-76786165 MS.gene48068:CDS 35.0%
! AGTAACTCACGTGTGTTTTA+TGG + chr5.3:76786450-76786469 MS.gene48068:CDS 35.0%
! CAATTGTCACTTGCTTACAA+TGG + chr5.3:76785772-76785791 MS.gene48068:CDS 35.0%
!! AGAAAAAGGTGTTCAACTTG+TGG + chr5.3:76786011-76786030 MS.gene48068:CDS 35.0%
!! ATACTTTGGTACTGATAGCT+TGG + chr5.3:76785960-76785979 MS.gene48068:CDS 35.0%
!!! CCAATTACTTGGTTTTGCTT+TGG + chr5.3:76785654-76785673 MS.gene48068:CDS 35.0%
AATGGTGACACAACAGATTG+TGG - chr5.3:76785253-76785272 None:intergenic 40.0%
ACCAAGATTATCAATGGAGG+AGG + chr5.3:76785523-76785542 MS.gene48068:CDS 40.0%
ACCATTCAAGAGTTCTGTCT+TGG + chr5.3:76785267-76785286 MS.gene48068:CDS 40.0%
CCATTCAAGAGTTCTGTCTT+GGG + chr5.3:76785268-76785287 MS.gene48068:CDS 40.0%
CGAAGGTTATCGTTAAACAC+GGG - chr5.3:76785556-76785575 None:intergenic 40.0%
CTTCTCTCAAATGTGAGATC+TGG + chr5.3:76785599-76785618 MS.gene48068:CDS 40.0%
GAAACAACACCATTACTGCT+AGG - chr5.3:76786093-76786112 None:intergenic 40.0%
GAAGGTTATCGTTAAACACG+GGG - chr5.3:76785555-76785574 None:intergenic 40.0%
GACAATTGAACAAAACCTGC+TGG - chr5.3:76785760-76785779 None:intergenic 40.0%
GCTGAAATTGCCATAACATC+TGG - chr5.3:76785805-76785824 None:intergenic 40.0%
GTTGAGTTTCCTGAACTAGT+TGG - chr5.3:76786399-76786418 None:intergenic 40.0%
TCCAATCAGGAGCAAATTTC+AGG + chr5.3:76786159-76786178 MS.gene48068:CDS 40.0%
TGAATCTGTTCAACCTCCTA+AGG + chr5.3:76786056-76786075 MS.gene48068:CDS 40.0%
TGCTGGTGAATGGAATAGAT+CGG - chr5.3:76785743-76785762 None:intergenic 40.0%
! ACCTGAAATTTGCTCCTGAT+TGG - chr5.3:76786163-76786182 None:intergenic 40.0%
! CTTCCTATGGACTTTGAAAG+TGG + chr5.3:76786372-76786391 MS.gene48068:CDS 40.0%
! TCCTCCTCCATTGATAATCT+TGG - chr5.3:76785527-76785546 None:intergenic 40.0%
! TTCACACTCACAACAGGTTT+TGG - chr5.3:76786258-76786277 None:intergenic 40.0%
!!! GCTTTTTTCGGAGTCTTTCT+CGG - chr5.3:76785860-76785879 None:intergenic 40.0%
AAGAGTTCTGTCTTGGGACA+TGG + chr5.3:76785274-76785293 MS.gene48068:CDS 45.0%
ACAACACCATTACTGCTAGG+TGG - chr5.3:76786090-76786109 None:intergenic 45.0%
ATATCCTGCTGCACCATCTT+TGG - chr5.3:76786294-76786313 None:intergenic 45.0%
CCCAAGACAGAACTCTTGAA+TGG - chr5.3:76785271-76785290 None:intergenic 45.0%
CTCATTCAGCAACGTATTCG+AGG - chr5.3:76786229-76786248 None:intergenic 45.0%
GAAAGTGGACCAACTAGTTC+AGG + chr5.3:76786387-76786406 MS.gene48068:CDS 45.0%
GAACAAAACCTGCTGGTGAA+TGG - chr5.3:76785753-76785772 None:intergenic 45.0%
GATCTATTCCATTCACCAGC+AGG + chr5.3:76785742-76785761 MS.gene48068:CDS 45.0%
GGTCCACTTTCAAAGTCCAT+AGG - chr5.3:76786378-76786397 None:intergenic 45.0%
TATTGAGCCAGAGCCAAAGA+TGG + chr5.3:76786278-76786297 MS.gene48068:CDS 45.0%
TGAGATCTGGATGCTTAGCA+GGG + chr5.3:76785612-76785631 MS.gene48068:CDS 45.0%
TGGTCCTAACACTTAGACGA+AGG - chr5.3:76785573-76785592 None:intergenic 45.0%
!! TGGTGTTGTTTCCTCTTCAG+TGG + chr5.3:76786101-76786120 MS.gene48068:CDS 45.0%
AAAAAAGCGAGTCGCACGTG+AGG + chr5.3:76785872-76785891 MS.gene48068:CDS 50.0%
AAAAAGCGAGTCGCACGTGA+GGG + chr5.3:76785873-76785892 MS.gene48068:CDS 50.0%
CTAGGTGGAGATGCAACCTT+AGG - chr5.3:76786075-76786094 None:intergenic 50.0%
GATGGTGCAGCAGGATATAG+TGG + chr5.3:76786296-76786315 MS.gene48068:CDS 50.0%
GCATCTCCACCTAGCAGTAA+TGG + chr5.3:76786081-76786100 MS.gene48068:CDS 50.0%
GTGAGATCTGGATGCTTAGC+AGG + chr5.3:76785611-76785630 MS.gene48068:CDS 50.0%
TGGCGCTTCACCAGATGTTA+TGG + chr5.3:76785792-76785811 MS.gene48068:CDS 50.0%
! TGTTGCAGTGTCGAATCCAG+AGG + chr5.3:76785375-76785394 MS.gene48068:CDS 50.0%
!!! CACGTGCGACTCGCTTTTTT+CGG - chr5.3:76785872-76785891 None:intergenic 50.0%
AGAGCCAAAGATGGTGCAGC+AGG + chr5.3:76786287-76786306 MS.gene48068:CDS 55.0%
GGTGGAGATGCAACCTTAGG+AGG - chr5.3:76786072-76786091 None:intergenic 55.0%
TGAGCTTGCAGCCACTGAAG+AGG - chr5.3:76786115-76786134 None:intergenic 55.0%
! TGCTGCACCATCTTTGGCTC+TGG - chr5.3:76786288-76786307 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 76785241 76786491 76785241 ID=MS.gene48068
chr5.3 mRNA 76785241 76786491 76785241 ID=MS.gene48068.t1;Parent=MS.gene48068
chr5.3 exon 76785241 76786491 76785241 ID=MS.gene48068.t1.exon1;Parent=MS.gene48068.t1
chr5.3 CDS 76785241 76786491 76785241 ID=cds.MS.gene48068.t1;Parent=MS.gene48068.t1
Gene Sequence

>MS.gene48068

ATGGAATCTCCACAATCTGTTGTGTCACCATTCAAGAGTTCTGTCTTGGGACATGGTGAGAAGTATAATTCTGATGTTTTGACACAAAGTTCTAGTCCTTTGTCTAAAGACATTGAGGTCAATGGAAAAGAAGCTGTTGCAGTGTCGAATCCAGAGGAATTTATTGGAGTAGTTGATGTTTACATACATCAAGCTAGGGACATTCATAACATCTGCATATACCATAAGCAAGATGTTTATGCTAAGATTTCTTTGACTAGTGATCCTGAAAATTTTGTCAATACCAAGATTATCAATGGAGGAGGAAGAAACCCCGTGTTTAACGATAACCTTCGTCTAAGTGTTAGGACCATTGATTCTTCTCTCAAATGTGAGATCTGGATGCTTAGCAGGGTGAAAAATTATCTTGAAGACCAATTACTTGGTTTTGCTTTGGTACCTTTGTCTGAAGTATTGATGAAAGATGGAAAACTAGAGAAAGAGTTCTCTCTTTCTTCGACCGATCTATTCCATTCACCAGCAGGTTTTGTTCAATTGTCACTTGCTTACAATGGCGCTTCACCAGATGTTATGGCAATTTCAGCAATGCCTAATACTGAATTGGACAGAAACGGCACCGAGAAAGACTCCGAAAAAAGCGAGTCGCACGTGAGGGATTTAGATAAAATAGAGTTCCCAAATCCAAAAATTGTAAATGAAGACCACTTAATGGTTTCAGAATACTTTGGTACTGATAGCTTGGCCACTACTGATACTGAAAATCTTAGTTCAGAAAAAGGTGTTCAACTTGTGGAAAGCTTCTCAGCATGTAGTGTTGAATCTGTTCAACCTCCTAAGGTTGCATCTCCACCTAGCAGTAATGGTGTTGTTTCCTCTTCAGTGGCTGCAAGCTCAGAATCACATGCTGCTACTAAATCTTCCAATCAGGAGCAAATTTCAGGTACAGAAGAGAAAAGAGTTGATCTCAAAGACGGCGAGAGTGATTCCTCGAATACGTTGCTGAATGAGTCATTTCCAAAACCTGTTGTGAGTGTGAATATTGAGCCAGAGCCAAAGATGGTGCAGCAGGATATAGTGGACATGTACTTGAAAAGTATGCAGCAATTTACCGAGTCATTAGCAAAAATGAAGCTTCCTATGGACTTTGAAAGTGGACCAACTAGTTCAGGAAACTCAACCTCTGAACAGAAACTACAATCTTCAAAGAGTAGTAACTCACGTGTGTTTTATGGTAGCAGAGCTTTCTTCTGA

Protein sequence

>MS.gene48068.t1

MESPQSVVSPFKSSVLGHGEKYNSDVLTQSSSPLSKDIEVNGKEAVAVSNPEEFIGVVDVYIHQARDIHNICIYHKQDVYAKISLTSDPENFVNTKIINGGGRNPVFNDNLRLSVRTIDSSLKCEIWMLSRVKNYLEDQLLGFALVPLSEVLMKDGKLEKEFSLSSTDLFHSPAGFVQLSLAYNGASPDVMAISAMPNTELDRNGTEKDSEKSESHVRDLDKIEFPNPKIVNEDHLMVSEYFGTDSLATTDTENLSSEKGVQLVESFSACSVESVQPPKVASPPSSNGVVSSSVAASSESHAATKSSNQEQISGTEEKRVDLKDGESDSSNTLLNESFPKPVVSVNIEPEPKMVQQDIVDMYLKSMQQFTESLAKMKLPMDFESGPTSSGNSTSEQKLQSSKSSNSRVFYGSRAFF