Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51626.t1 | XP_003598272.2 | 99.6 | 565 | 2 | 0 | 1 | 565 | 180 | 744 | 1.50E-230 | 808.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51626.t1 | Q9FMA3 | 71.6 | 566 | 146 | 5 | 1 | 564 | 176 | 728 | 2.1e-145 | 517.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51626.t1 | G7J0B8 | 99.6 | 565 | 2 | 0 | 1 | 565 | 180 | 744 | 1.1e-230 | 808.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51626 | MS.gene57427 | PPI |
| MS.gene28100 | MS.gene51626 | PPI |
| MS.gene051559 | MS.gene51626 | PPI |
| MS.gene51626 | MS.gene33044 | PPI |
| MS.gene51626 | MS.gene030781 | PPI |
| MS.gene51626 | MS.gene52445 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51626.t1 | MTR_3g009230 | 99.469 | 565 | 3 | 0 | 1 | 565 | 180 | 744 | 0.0 | 1159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51626.t1 | AT5G56290 | 72.792 | 566 | 139 | 6 | 1 | 564 | 176 | 728 | 0.0 | 838 |
Find 134 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGCAATCGTTCCATATTT+TGG | 0.120283 | 3.4:+4251990 | None:intergenic |
| AATGGATGAACCTGAAATTT+AGG | 0.161869 | 3.4:+4255691 | None:intergenic |
| TGCCATGCATTCTCTGCTTT+TGG | 0.180441 | 3.4:+4247189 | None:intergenic |
| GCTGCTTTGAAGTATCTATT+TGG | 0.188621 | 3.4:-4252029 | MS.gene51626:CDS |
| TCAAGCTCCCTATGTCTTTC+AGG | 0.198982 | 3.4:+4256900 | None:intergenic |
| GGGAAGGCTCTTCGGATAAT+TGG | 0.210063 | 3.4:-4254013 | MS.gene51626:CDS |
| TGTTCGTGCTTGGGCAAATA+TGG | 0.211852 | 3.4:-4248169 | MS.gene51626:CDS |
| GAAATTTAGGGTCAGCATTC+TGG | 0.213774 | 3.4:+4255704 | None:intergenic |
| GAATGCTGACCCTAAATTTC+AGG | 0.215296 | 3.4:-4255701 | MS.gene51626:intron |
| TTACCGAATTCAGTCAAAAT+AGG | 0.221138 | 3.4:-4256815 | MS.gene51626:CDS |
| AGCTTGTGATAGTCGTAATT+TGG | 0.252542 | 3.4:-4246981 | MS.gene51626:CDS |
| ATAGGGAGCTTGAAGATTAT+TGG | 0.278533 | 3.4:-4256890 | MS.gene51626:CDS |
| CTACGGAATCACCCAAAATA+TGG | 0.297361 | 3.4:-4252002 | MS.gene51626:CDS |
| AAGCATGAGCACCCTGTATC+AGG | 0.323226 | 3.4:+4256850 | None:intergenic |
| TGGTGAATTCTTAAATAATA+AGG | 0.327549 | 3.4:-4254628 | MS.gene51626:intron |
| GCAGATGTTCACATAGTTCT+TGG | 0.334364 | 3.4:-4249972 | MS.gene51626:CDS |
| CAGATTGGTGAAGGAGCCTT+TGG | 0.341807 | 3.4:-4254035 | MS.gene51626:CDS |
| GCCCAAGCACGAACATAATT+AGG | 0.346674 | 3.4:+4248177 | None:intergenic |
| GCCCTTTCCTGAACAGATCT+TGG | 0.357838 | 3.4:+4253228 | None:intergenic |
| GCAACAGAAAGACAACAAAA+TGG | 0.369453 | 3.4:-4254131 | MS.gene51626:CDS |
| ATTGTAGAGGAAACTCCTTC+TGG | 0.376901 | 3.4:+4246951 | None:intergenic |
| AAATTTAGGGTCAGCATTCT+GGG | 0.380235 | 3.4:+4255705 | None:intergenic |
| CATTACCCGCACCTGGAGAT+TGG | 0.384047 | 3.4:-4254695 | MS.gene51626:CDS |
| AAGAAGGCCAAGATCTGTTC+AGG | 0.386955 | 3.4:-4253235 | MS.gene51626:CDS |
| AATTCATTCGCCCACAGTTC+AGG | 0.386982 | 3.4:+4254155 | None:intergenic |
| ACATGTTCATACCTCGCATT+TGG | 0.389527 | 3.4:+4255776 | None:intergenic |
| TTGGAGGTGCTTCTTGCTCT+TGG | 0.391890 | 3.4:-4252455 | MS.gene51626:CDS |
| CAGATGTTCACATAGTTCTT+GGG | 0.395395 | 3.4:-4249971 | MS.gene51626:CDS |
| GCCTAATTATGTTCGTGCTT+GGG | 0.397467 | 3.4:-4248178 | MS.gene51626:CDS |
| GGAAGGCTCTTCGGATAATT+GGG | 0.397858 | 3.4:-4254012 | MS.gene51626:CDS |
| ATGGATGAACCTGAAATTTA+GGG | 0.400523 | 3.4:+4255692 | None:intergenic |
| AATGACTGGGCTGAGGAATT+CGG | 0.406128 | 3.4:-4254062 | MS.gene51626:CDS |
| AGCCTAATTATGTTCGTGCT+TGG | 0.409800 | 3.4:-4248179 | MS.gene51626:CDS |
| AGAAGCACCTCCAAGTTTGC+TGG | 0.421887 | 3.4:+4252464 | None:intergenic |
| CCCTATGTCTTTCAGGCATA+GGG | 0.427238 | 3.4:+4256907 | None:intergenic |
| TACCGAATTCAGTCAAAATA+GGG | 0.427648 | 3.4:-4256814 | MS.gene51626:CDS |
| AGCCTTTGGGGAAGGCTCTT+CGG | 0.429856 | 3.4:-4254021 | MS.gene51626:CDS |
| AGAGGCTGATCCAGCAAACT+TGG | 0.430306 | 3.4:-4252474 | MS.gene51626:CDS |
| CAAAGTTGCATGTTAATGAC+TGG | 0.437634 | 3.4:-4254076 | MS.gene51626:CDS |
| TGCAATAGCGGCTTATCAAC+AGG | 0.447194 | 3.4:-4249814 | MS.gene51626:intron |
| GTTCGTGCTTGGGCAAATAT+GGG | 0.457058 | 3.4:-4248168 | MS.gene51626:CDS |
| AGCCACAAGATTACTCTCTC+TGG | 0.457405 | 3.4:-4249881 | MS.gene51626:CDS |
| AGATTGGTGAAGGAGCCTTT+GGG | 0.458135 | 3.4:-4254034 | MS.gene51626:CDS |
| TATTCTGTTGCCCAATCTCC+AGG | 0.464052 | 3.4:+4254684 | None:intergenic |
| AGAAAATGACGATGATCAAC+AGG | 0.466750 | 3.4:-4253114 | MS.gene51626:intron |
| GACAGCAATGTCCGTGGCAA+GGG | 0.467141 | 3.4:-4256930 | MS.gene51626:CDS |
| AACAGCATGGTGCAAATGGT+TGG | 0.468693 | 3.4:-4256749 | MS.gene51626:CDS |
| ATTACCCGCACCTGGAGATT+GGG | 0.468992 | 3.4:-4254694 | MS.gene51626:CDS |
| CATGTCCAACATTGCTGCTA+TGG | 0.469646 | 3.4:-4255758 | MS.gene51626:CDS |
| TCCGAGTAGTCTCCATCCTT+CGG | 0.469842 | 3.4:+4253148 | None:intergenic |
| GCCAAGATCTGTTCAGGAAA+GGG | 0.474799 | 3.4:-4253229 | MS.gene51626:CDS |
| GTCTCAAGGAAACCTGATAC+AGG | 0.475756 | 3.4:-4256862 | MS.gene51626:CDS |
| GGATTTGGATGACCAACATA+AGG | 0.481106 | 3.4:+4253263 | None:intergenic |
| ACCCTGAAAATTCCGAAGGA+TGG | 0.481367 | 3.4:-4253160 | MS.gene51626:CDS |
| ATGTGAACATCTGCATCATC+AGG | 0.485344 | 3.4:+4249981 | None:intergenic |
| CACCATGATTATACTGCTGC+TGG | 0.487665 | 3.4:+4254661 | None:intergenic |
| AGCAGCGGTCAGATAGTTCA+AGG | 0.500097 | 3.4:-4253313 | MS.gene51626:CDS |
| ACAGCATGGTGCAAATGGTT+GGG | 0.501619 | 3.4:-4256748 | MS.gene51626:CDS |
| TGAATTTCCTTACTTGAGCA+TGG | 0.502258 | 3.4:+4257138 | None:intergenic |
| CTGCTCCATAGCAGCAATGT+TGG | 0.502786 | 3.4:+4255753 | None:intergenic |
| TACTCTCTCTGGAACAAGCT+TGG | 0.503008 | 3.4:-4249870 | MS.gene51626:CDS |
| GAGTCTGCATTACCCGCACC+TGG | 0.505998 | 3.4:-4254702 | MS.gene51626:CDS |
| ATTCTCTTGATAAGTTGTAC+AGG | 0.510273 | 3.4:+4249946 | None:intergenic |
| CTTTGAAGTATCTATTTGGA+TGG | 0.512648 | 3.4:-4252025 | MS.gene51626:CDS |
| AGTATAATCATGGTGATGCA+TGG | 0.513119 | 3.4:-4254653 | MS.gene51626:CDS |
| GAGGAATTCGGTCAGCAGAT+TGG | 0.513609 | 3.4:-4254050 | MS.gene51626:CDS |
| TCATCTTTGATACAAATTGA+AGG | 0.516310 | 3.4:+4254763 | None:intergenic |
| GAGCAACAGCATGGTGCAAA+TGG | 0.516659 | 3.4:-4256753 | MS.gene51626:CDS |
| TCAGATTTAAATCCTTATGT+TGG | 0.516785 | 3.4:-4253275 | MS.gene51626:CDS |
| TGAACTATCTGACCGCTGCT+TGG | 0.519477 | 3.4:+4253316 | None:intergenic |
| AATGAGTTAGCCCATGCATC+GGG | 0.521792 | 3.4:+4256777 | None:intergenic |
| CTCTTCGGATAATTGGGCTC+AGG | 0.525195 | 3.4:-4254006 | MS.gene51626:CDS |
| AGTATCTATTTGGATGGCTA+CGG | 0.525996 | 3.4:-4252019 | MS.gene51626:CDS |
| CAGGCATAGGGCCCTTGCCA+CGG | 0.528217 | 3.4:+4256919 | None:intergenic |
| TTGAAGGCTTATCCTAGAGC+TGG | 0.529807 | 3.4:-4254179 | MS.gene51626:intron |
| GATATGATAATCCCGATGCA+TGG | 0.532117 | 3.4:-4256788 | MS.gene51626:CDS |
| CTTGGTGTCAGTCATACAAA+TGG | 0.532297 | 3.4:-4252437 | MS.gene51626:intron |
| GGCTGATCCAGCAAACTTGG+AGG | 0.534867 | 3.4:-4252471 | MS.gene51626:CDS |
| TACCAGCAGCAGTATAATCA+TGG | 0.535305 | 3.4:-4254663 | MS.gene51626:CDS |
| GGTGAAGGAGCCTTTGGGGA+AGG | 0.535807 | 3.4:-4254029 | MS.gene51626:CDS |
| TCCCTATGTCTTTCAGGCAT+AGG | 0.536253 | 3.4:+4256906 | None:intergenic |
| GTGCTCATGCTTCAGACGGA+TGG | 0.538207 | 3.4:-4256839 | MS.gene51626:CDS |
| TGCCTGTTCAAAAGCTGCAA+TGG | 0.538654 | 3.4:+4249914 | None:intergenic |
| CCCTATGCCTGAAAGACATA+GGG | 0.542005 | 3.4:-4256907 | MS.gene51626:CDS |
| CACAGTTCAGGTCCAGCTCT+AGG | 0.544577 | 3.4:+4254167 | None:intergenic |
| TTCCAGAGAGAGTAATCTTG+TGG | 0.546481 | 3.4:+4249879 | None:intergenic |
| TTCGGTCAGCAGATTGGTGA+AGG | 0.546573 | 3.4:-4254044 | MS.gene51626:CDS |
| ATCCAAAAGCAGAGAATGCA+TGG | 0.548861 | 3.4:-4247191 | MS.gene51626:CDS |
| CATCCAAATCCACTAAAAGA+AGG | 0.550824 | 3.4:-4253251 | MS.gene51626:CDS |
| AATATTTGAGAATTTCATTG+AGG | 0.551469 | 3.4:-4247167 | MS.gene51626:intron |
| GGCCAAGATCTGTTCAGGAA+AGG | 0.557037 | 3.4:-4253230 | MS.gene51626:CDS |
| GAGGAAACTCCTTCTGGAGA+AGG | 0.560805 | 3.4:+4246957 | None:intergenic |
| GTTGCCCAATCTCCAGGTGC+GGG | 0.560914 | 3.4:+4254690 | None:intergenic |
| TGACAGCAATGTCCGTGGCA+AGG | 0.561792 | 3.4:-4256931 | MS.gene51626:CDS |
| AGCAAGTTGCTGCCAAGCAG+CGG | 0.561910 | 3.4:-4253328 | MS.gene51626:CDS |
| ATATGATAATCCCGATGCAT+GGG | 0.561935 | 3.4:-4256787 | MS.gene51626:CDS |
| AAAGAATCAGACATCTCAGA+TGG | 0.562845 | 3.4:+4251969 | None:intergenic |
| AAATGAGTTAGCCCATGCAT+CGG | 0.570079 | 3.4:+4256776 | None:intergenic |
| TCCGAAGGATGGAGACTACT+CGG | 0.572229 | 3.4:-4253149 | MS.gene51626:CDS |
| GCCCTATGCCTGAAAGACAT+AGG | 0.572462 | 3.4:-4256908 | MS.gene51626:CDS |
| TAATTTGGACCTTCTCCAGA+AGG | 0.577440 | 3.4:-4246966 | MS.gene51626:CDS |
| TGTTGCCCAATCTCCAGGTG+CGG | 0.578121 | 3.4:+4254689 | None:intergenic |
| AGCGATGATGCGTGCACAAG+AGG | 0.579422 | 3.4:-4252492 | MS.gene51626:CDS |
| TGCTTCAAGGAATGACATGT+TGG | 0.586068 | 3.4:-4247005 | MS.gene51626:CDS |
| TAGCACAAAACTTATTGTAG+AGG | 0.599270 | 3.4:+4246938 | None:intergenic |
| AGATGTTCACATAGTTCTTG+GGG | 0.599275 | 3.4:-4249970 | MS.gene51626:CDS |
| TTGAGAATTCATCAACCCAC+TGG | 0.600819 | 3.4:+4254096 | None:intergenic |
| AAGAATCAGACATCTCAGAT+GGG | 0.606232 | 3.4:+4251970 | None:intergenic |
| AATCATGGTGATGCATGGGC+TGG | 0.607937 | 3.4:-4254648 | MS.gene51626:CDS |
| GGGTATCAGTTATGCAAACC+AGG | 0.609130 | 3.4:-4248148 | MS.gene51626:intron |
| AACTCATTTGAGCAACAGCA+TGG | 0.613586 | 3.4:-4256762 | MS.gene51626:CDS |
| TAGAGCTGGACCTGAACTGT+GGG | 0.615883 | 3.4:-4254165 | MS.gene51626:CDS |
| ATCCGAAGAGCCTTCCCCAA+AGG | 0.620364 | 3.4:+4254019 | None:intergenic |
| CAGGGTGCTCATGCTTCAGA+CGG | 0.622096 | 3.4:-4256843 | MS.gene51626:CDS |
| GCAGCGGTCAGATAGTTCAA+GGG | 0.629171 | 3.4:-4253312 | MS.gene51626:CDS |
| ACCGTAGCAGCATAATGGCT+AGG | 0.631322 | 3.4:-4257193 | None:intergenic |
| GATTATTGGAATGAGTCTCA+AGG | 0.633092 | 3.4:-4256876 | MS.gene51626:CDS |
| GTATAATCATGGTGATGCAT+GGG | 0.633154 | 3.4:-4254652 | MS.gene51626:CDS |
| AAAGTTGCATGTTAATGACT+GGG | 0.637762 | 3.4:-4254075 | MS.gene51626:CDS |
| GATTGGTGAAGGAGCCTTTG+GGG | 0.640785 | 3.4:-4254033 | MS.gene51626:CDS |
| CAGCGGTCAGATAGTTCAAG+GGG | 0.645860 | 3.4:-4253311 | MS.gene51626:CDS |
| ATTGCTGTCAATATCTAGAG+AGG | 0.646477 | 3.4:+4256944 | None:intergenic |
| TCAAAGTGCTGATGCAATAG+CGG | 0.646885 | 3.4:-4249826 | MS.gene51626:CDS |
| ATGGAGCAACTGATGACCAG+TGG | 0.653786 | 3.4:-4254112 | MS.gene51626:CDS |
| CTAGAGCTGGACCTGAACTG+TGG | 0.658476 | 3.4:-4254166 | MS.gene51626:CDS |
| GCATGTTAATGACTGGGCTG+AGG | 0.661649 | 3.4:-4254069 | MS.gene51626:CDS |
| TTTGTATCAAAGATGAGCCG+TGG | 0.664730 | 3.4:-4254756 | MS.gene51626:CDS |
| TCTCAAGGAAACCTGATACA+GGG | 0.670691 | 3.4:-4256861 | MS.gene51626:CDS |
| GATATTGACAGCAATGTCCG+TGG | 0.674753 | 3.4:-4256936 | MS.gene51626:CDS |
| AGAATCAGACATCTCAGATG+GGG | 0.675113 | 3.4:+4251971 | None:intergenic |
| CATCGATTATCAATTCGCCA+CGG | 0.682681 | 3.4:+4254739 | None:intergenic |
| TGGAGCAACTGATGACCAGT+GGG | 0.683759 | 3.4:-4254111 | MS.gene51626:CDS |
| TCGTATGCTTGCAAATACGT+TGG | 0.700626 | 3.4:-4255728 | MS.gene51626:CDS |
| TCATCAGTAGACCAAATGCG+AGG | 0.722353 | 3.4:-4255787 | MS.gene51626:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 4246949 | 4257201 | 4246949 | ID=MS.gene51626 |
| chr3.4 | mRNA | 4246949 | 4257201 | 4246949 | ID=MS.gene51626.t1;Parent=MS.gene51626 |
| chr3.4 | exon | 4257151 | 4257201 | 4257151 | ID=MS.gene51626.t1.exon1;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4257151 | 4257201 | 4257151 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4256731 | 4256982 | 4256731 | ID=MS.gene51626.t1.exon2;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4256731 | 4256982 | 4256731 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4255702 | 4255818 | 4255702 | ID=MS.gene51626.t1.exon3;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4255702 | 4255818 | 4255702 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4254629 | 4254796 | 4254629 | ID=MS.gene51626.t1.exon4;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4254629 | 4254796 | 4254629 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4253996 | 4254195 | 4253996 | ID=MS.gene51626.t1.exon5;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4253996 | 4254195 | 4253996 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4253115 | 4253361 | 4253115 | ID=MS.gene51626.t1.exon6;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4253115 | 4253361 | 4253115 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4252438 | 4252522 | 4252438 | ID=MS.gene51626.t1.exon7;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4252438 | 4252522 | 4252438 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4251956 | 4252065 | 4251956 | ID=MS.gene51626.t1.exon8;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4251956 | 4252065 | 4251956 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4249815 | 4250039 | 4249815 | ID=MS.gene51626.t1.exon9;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4249815 | 4250039 | 4249815 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4248149 | 4248214 | 4248149 | ID=MS.gene51626.t1.exon10;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4248149 | 4248214 | 4248149 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4247168 | 4247262 | 4247168 | ID=MS.gene51626.t1.exon11;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4247168 | 4247262 | 4247168 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| chr3.4 | exon | 4246949 | 4247030 | 4246949 | ID=MS.gene51626.t1.exon12;Parent=MS.gene51626.t1 |
| chr3.4 | CDS | 4246949 | 4247030 | 4246949 | ID=cds.MS.gene51626.t1;Parent=MS.gene51626.t1 |
| Gene Sequence |
| Protein sequence |
