Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52935.t1 | XP_003591797.1 | 77.7 | 238 | 24 | 2 | 1 | 209 | 129 | 366 | 5.00E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52935.t1 | P13917 | 33.5 | 233 | 130 | 8 | 1 | 209 | 139 | 370 | 1.3e-23 | 111.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52935.t1 | G7I467 | 77.7 | 238 | 24 | 2 | 1 | 209 | 129 | 366 | 3.6e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049573 | MS.gene52935 | 0.834542 | 2.82E-56 | -1.69E-46 |
| MS.gene05141 | MS.gene52935 | 0.849337 | 3.45E-60 | -1.69E-46 |
| MS.gene05142 | MS.gene52935 | 0.822629 | 2.14E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene52935 | 0.868935 | 4.51E-66 | -1.69E-46 |
| MS.gene057014 | MS.gene52935 | 0.841958 | 3.46E-58 | -1.69E-46 |
| MS.gene059236 | MS.gene52935 | 0.822839 | 1.91E-53 | -1.69E-46 |
| MS.gene059547 | MS.gene52935 | 0.80427 | 2.34E-49 | -1.69E-46 |
| MS.gene061226 | MS.gene52935 | 0.807581 | 4.71E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGAAATCTATTAGAGTTTC+TGG | 0.165523 | 1.2:-63743001 | MS.gene52935:CDS |
| AGGATTAACGGTGCTAATTT+TGG | 0.222002 | 1.2:+63742919 | None:intergenic |
| AATAAATTTGCAATTTGTTT+AGG | 0.256299 | 1.2:-63743177 | MS.gene52935:CDS |
| TGGTTCCACCAAATCCATTT+TGG | 0.303617 | 1.2:+63742939 | None:intergenic |
| TCTATAGACCAAAATGGATT+TGG | 0.304145 | 1.2:-63742947 | MS.gene52935:CDS |
| GAATGTGGTTGGAATATTAT+TGG | 0.315106 | 1.2:-63742720 | MS.gene52935:CDS |
| GAACTTGGCGCAGTGCTAAC+AGG | 0.339315 | 1.2:+63743061 | None:intergenic |
| TCCACAAATGGAAACTATTC+TGG | 0.339835 | 1.2:-63743348 | MS.gene52935:CDS |
| CGTTAATCCTTACACAATAA+TGG | 0.348904 | 1.2:-63742906 | MS.gene52935:CDS |
| ACCAGAATAGTTTCCATTTG+TGG | 0.353898 | 1.2:+63743347 | None:intergenic |
| TGATAAGGATGTGATTTGTT+TGG | 0.378748 | 1.2:-63742673 | MS.gene52935:CDS |
| GTTGAACCTGTAGCACCATT+TGG | 0.387509 | 1.2:-63742824 | MS.gene52935:CDS |
| CATTATTGTGTAAGGATTAA+CGG | 0.392138 | 1.2:+63742907 | None:intergenic |
| GATAAGGATGTGATTTGTTT+GGG | 0.395759 | 1.2:-63742672 | None:intergenic |
| GAAGACTCCATTATTGTGTA+AGG | 0.405084 | 1.2:+63742899 | None:intergenic |
| AAATCAGATGAGTATTTCAT+TGG | 0.428391 | 1.2:-63743025 | MS.gene52935:intron |
| GTCAAAGGTATGGCTGGTCT+TGG | 0.431367 | 1.2:-63743252 | MS.gene52935:CDS |
| GGTTCAAGTGTTGTACAAAA+TGG | 0.447374 | 1.2:-63743288 | MS.gene52935:CDS |
| GTTTCAGTTCAATCCACAAA+TGG | 0.450626 | 1.2:-63743360 | MS.gene52935:CDS |
| CATGTTCCAAATGGTGCTAC+AGG | 0.465297 | 1.2:+63742818 | None:intergenic |
| CTAACAGGGTTTGTAATAAG+AGG | 0.475903 | 1.2:+63743076 | None:intergenic |
| AATGGTTCGTATTAATGATA+AGG | 0.488541 | 1.2:-63742688 | MS.gene52935:CDS |
| ATAGACCAAAATGGATTTGG+TGG | 0.508375 | 1.2:-63742944 | MS.gene52935:CDS |
| AAGGGTGTCAAAGGTATGGC+TGG | 0.510377 | 1.2:-63743258 | MS.gene52935:CDS |
| ATTTGTTTAGGTACTCAAGA+TGG | 0.523453 | 1.2:-63743165 | MS.gene52935:CDS |
| TGCTAAGGGTGTCAAAGGTA+TGG | 0.525240 | 1.2:-63743262 | MS.gene52935:CDS |
| TCATCAGTGTTCAATAGATA+AGG | 0.532661 | 1.2:+63743120 | None:intergenic |
| GGTCTTGCTAAGGGTGTCAA+AGG | 0.536708 | 1.2:-63743267 | MS.gene52935:CDS |
| CTTGCAAAACATGTTCCAAA+TGG | 0.563050 | 1.2:+63742809 | None:intergenic |
| AACTTGGCGCAGTGCTAACA+GGG | 0.570600 | 1.2:+63743062 | None:intergenic |
| AAGGTATGGCTGGTCTTGGA+AGG | 0.572528 | 1.2:-63743248 | MS.gene52935:CDS |
| TTGCAGAATGAGAATGTGGT+TGG | 0.581262 | 1.2:-63742731 | MS.gene52935:intron |
| TGTACAAAATGGTCTTGCTA+AGG | 0.594025 | 1.2:-63743277 | MS.gene52935:CDS |
| AAAGCTGATGAAAATTGTGA+AGG | 0.597985 | 1.2:+63743210 | None:intergenic |
| TATTATTGGAGCTAATGCAA+TGG | 0.621482 | 1.2:-63742706 | MS.gene52935:CDS |
| GTACAAAATGGTCTTGCTAA+GGG | 0.632901 | 1.2:-63743276 | MS.gene52935:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAATTTGCAATTTGTTT+AGG | - | chr1.2:63742861-63742880 | MS.gene52935:CDS | 15.0% |
| !!! | ATTTTGGTCTATAGATAAAA+GGG | + | chr1.2:63743086-63743105 | None:intergenic | 20.0% |
| ! | AAATCAGATGAGTATTTCAT+TGG | - | chr1.2:63743013-63743032 | MS.gene52935:CDS | 25.0% |
| ! | AATGGTTCGTATTAATGATA+AGG | - | chr1.2:63743350-63743369 | MS.gene52935:CDS | 25.0% |
| ! | CATTATTGTGTAAGGATTAA+CGG | + | chr1.2:63743134-63743153 | None:intergenic | 25.0% |
| ! | CCACAAATAAAAAGAAAGTT+AGG | + | chr1.2:63742732-63742751 | None:intergenic | 25.0% |
| !! | CTTTTATCTATAGACCAAAA+TGG | - | chr1.2:63743085-63743104 | MS.gene52935:CDS | 25.0% |
| !!! | CATTTTGGTCTATAGATAAA+AGG | + | chr1.2:63743087-63743106 | None:intergenic | 25.0% |
| !!! | CCTAACTTTCTTTTTATTTG+TGG | - | chr1.2:63742729-63742748 | MS.gene52935:intron | 25.0% |
| AAAGCTGATGAAAATTGTGA+AGG | + | chr1.2:63742831-63742850 | None:intergenic | 30.0% | |
| ATTTGTTTAGGTACTCAAGA+TGG | - | chr1.2:63742873-63742892 | MS.gene52935:CDS | 30.0% | |
| CGTTAATCCTTACACAATAA+TGG | - | chr1.2:63743132-63743151 | MS.gene52935:CDS | 30.0% | |
| GAATGTGGTTGGAATATTAT+TGG | - | chr1.2:63743318-63743337 | MS.gene52935:CDS | 30.0% | |
| GTGAAATCTATTAGAGTTTC+TGG | - | chr1.2:63743037-63743056 | MS.gene52935:intron | 30.0% | |
| TATTATTGGAGCTAATGCAA+TGG | - | chr1.2:63743332-63743351 | MS.gene52935:CDS | 30.0% | |
| TCATCAGTGTTCAATAGATA+AGG | + | chr1.2:63742921-63742940 | None:intergenic | 30.0% | |
| ! | AAGCATTAGTTCTTCAAGAA+TGG | - | chr1.2:63743255-63743274 | MS.gene52935:CDS | 30.0% |
| ! | AGCATTAGTTCTTCAAGAAT+GGG | - | chr1.2:63743256-63743275 | MS.gene52935:CDS | 30.0% |
| ! | TCTATAGACCAAAATGGATT+TGG | - | chr1.2:63743091-63743110 | MS.gene52935:CDS | 30.0% |
| ! | TTTTCACCTAAGAAAGAACT+TGG | + | chr1.2:63742995-63743014 | None:intergenic | 30.0% |
| !! | TGATAAGGATGTGATTTGTT+TGG | - | chr1.2:63743365-63743384 | MS.gene52935:CDS | 30.0% |
| ACCAGAATAGTTTCCATTTG+TGG | + | chr1.2:63742694-63742713 | None:intergenic | 35.0% | |
| ATTCTGCAACACAAGATCAA+TGG | + | chr1.2:63743296-63743315 | None:intergenic | 35.0% | |
| CTAACAGGGTTTGTAATAAG+AGG | + | chr1.2:63742965-63742984 | None:intergenic | 35.0% | |
| CTTGCAAAACATGTTCCAAA+TGG | + | chr1.2:63743232-63743251 | None:intergenic | 35.0% | |
| GAAGACTCCATTATTGTGTA+AGG | + | chr1.2:63743142-63743161 | None:intergenic | 35.0% | |
| GTTTCAGTTCAATCCACAAA+TGG | - | chr1.2:63742678-63742697 | MS.gene52935:CDS | 35.0% | |
| TCCACAAATGGAAACTATTC+TGG | - | chr1.2:63742690-63742709 | MS.gene52935:CDS | 35.0% | |
| ! | ATAGACCAAAATGGATTTGG+TGG | - | chr1.2:63743094-63743113 | MS.gene52935:CDS | 35.0% |
| ! | CAAGATGGTGTACTTTTCTT+TGG | - | chr1.2:63742888-63742907 | MS.gene52935:CDS | 35.0% |
| ! | GGTTCAAGTGTTGTACAAAA+TGG | - | chr1.2:63742750-63742769 | MS.gene52935:intron | 35.0% |
| ! | GTACAAAATGGTCTTGCTAA+GGG | - | chr1.2:63742762-63742781 | MS.gene52935:intron | 35.0% |
| ! | TGTACAAAATGGTCTTGCTA+AGG | - | chr1.2:63742761-63742780 | MS.gene52935:intron | 35.0% |
| !! | AGGATTAACGGTGCTAATTT+TGG | + | chr1.2:63743122-63743141 | None:intergenic | 35.0% |
| AGATCAATGGAAGGAACATC+TGG | + | chr1.2:63743283-63743302 | None:intergenic | 40.0% | |
| TGCAACACAAGATCAATGGA+AGG | + | chr1.2:63743292-63743311 | None:intergenic | 40.0% | |
| TGTGTTGCAGAATGAGAATG+TGG | - | chr1.2:63743303-63743322 | MS.gene52935:CDS | 40.0% | |
| TTGCAGAATGAGAATGTGGT+TGG | - | chr1.2:63743307-63743326 | MS.gene52935:CDS | 40.0% | |
| ! | GGTGTACTTTTCTTTGGTGA+TGG | - | chr1.2:63742894-63742913 | MS.gene52935:CDS | 40.0% |
| ! | TGGTTCCACCAAATCCATTT+TGG | + | chr1.2:63743102-63743121 | None:intergenic | 40.0% |
| ACTGCGCCAAGTTCTTTCTT+AGG | - | chr1.2:63742986-63743005 | MS.gene52935:CDS | 45.0% | |
| GTTGAACCTGTAGCACCATT+TGG | - | chr1.2:63743214-63743233 | MS.gene52935:CDS | 45.0% | |
| !! | CATGTTCCAAATGGTGCTAC+AGG | + | chr1.2:63743223-63743242 | None:intergenic | 45.0% |
| !! | TGCTAAGGGTGTCAAAGGTA+TGG | - | chr1.2:63742776-63742795 | MS.gene52935:intron | 45.0% |
| AACTTGGCGCAGTGCTAACA+GGG | + | chr1.2:63742979-63742998 | None:intergenic | 50.0% | |
| !! | AAGGGTGTCAAAGGTATGGC+TGG | - | chr1.2:63742780-63742799 | MS.gene52935:intron | 50.0% |
| !! | AAGGTATGGCTGGTCTTGGA+AGG | - | chr1.2:63742790-63742809 | MS.gene52935:intron | 50.0% |
| !! | GGTCTTGCTAAGGGTGTCAA+AGG | - | chr1.2:63742771-63742790 | MS.gene52935:intron | 50.0% |
| !! | GTCAAAGGTATGGCTGGTCT+TGG | - | chr1.2:63742786-63742805 | MS.gene52935:intron | 50.0% |
| GAACTTGGCGCAGTGCTAAC+AGG | + | chr1.2:63742980-63742999 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 63742673 | 63743387 | 63742673 | ID=MS.gene52935 |
| chr1.2 | mRNA | 63742673 | 63743387 | 63742673 | ID=MS.gene52935.t1;Parent=MS.gene52935 |
| chr1.2 | exon | 63743065 | 63743387 | 63743065 | ID=MS.gene52935.t1.exon1;Parent=MS.gene52935.t1 |
| chr1.2 | CDS | 63743065 | 63743387 | 63743065 | ID=cds.MS.gene52935.t1;Parent=MS.gene52935.t1 |
| chr1.2 | exon | 63742798 | 63743035 | 63742798 | ID=MS.gene52935.t1.exon2;Parent=MS.gene52935.t1 |
| chr1.2 | CDS | 63742798 | 63743035 | 63742798 | ID=cds.MS.gene52935.t1;Parent=MS.gene52935.t1 |
| chr1.2 | exon | 63742673 | 63742741 | 63742673 | ID=MS.gene52935.t1.exon3;Parent=MS.gene52935.t1 |
| chr1.2 | CDS | 63742673 | 63742741 | 63742673 | ID=cds.MS.gene52935.t1;Parent=MS.gene52935.t1 |
| Gene Sequence |
| Protein sequence |
