Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05142.t1 | XP_013460468.1 | 95.4 | 219 | 10 | 0 | 2 | 220 | 6 | 224 | 5.60E-125 | 456.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05142.t1 | P25317 | 70.3 | 219 | 65 | 0 | 1 | 219 | 1 | 219 | 6.3e-93 | 341.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05142.t1 | I3S5Q9 | 95.4 | 219 | 10 | 0 | 2 | 220 | 6 | 224 | 4.0e-125 | 456.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049573 | MS.gene05142 | 0.899595 | 1.70E-77 | -1.69E-46 |
| MS.gene05141 | MS.gene05142 | 0.832265 | 1.04E-55 | -1.69E-46 |
| MS.gene05142 | MS.gene051946 | 0.82682 | 2.20E-54 | -1.69E-46 |
| MS.gene05142 | MS.gene053549 | 0.805128 | 1.55E-49 | -1.69E-46 |
| MS.gene05142 | MS.gene056310 | 0.803475 | 3.42E-49 | -1.69E-46 |
| MS.gene05142 | MS.gene056311 | 0.838001 | 3.73E-57 | -1.69E-46 |
| MS.gene05142 | MS.gene057014 | 0.827701 | 1.35E-54 | -1.69E-46 |
| MS.gene05142 | MS.gene059236 | 0.807425 | 5.09E-50 | -1.69E-46 |
| MS.gene05142 | MS.gene065465 | 0.805635 | 1.21E-49 | -1.69E-46 |
| MS.gene05142 | MS.gene07397 | 0.866153 | 3.51E-65 | -1.69E-46 |
| MS.gene05142 | MS.gene21575 | 0.818721 | 1.69E-52 | -1.69E-46 |
| MS.gene05142 | MS.gene28555 | 0.802939 | 4.42E-49 | -1.69E-46 |
| MS.gene05142 | MS.gene29310 | 0.800704 | 1.27E-48 | -1.69E-46 |
| MS.gene05142 | MS.gene32567 | 0.812667 | 3.78E-51 | -1.69E-46 |
| MS.gene05142 | MS.gene40835 | 0.810905 | 9.14E-51 | -1.69E-46 |
| MS.gene05142 | MS.gene44720 | 0.815366 | 9.59E-52 | -1.69E-46 |
| MS.gene05142 | MS.gene44721 | 0.833476 | 5.22E-56 | -1.69E-46 |
| MS.gene05142 | MS.gene44722 | 0.810674 | 1.03E-50 | -1.69E-46 |
| MS.gene05142 | MS.gene44723 | 0.811977 | 5.35E-51 | -1.69E-46 |
| MS.gene05142 | MS.gene49887 | 0.817973 | 2.50E-52 | -1.69E-46 |
| MS.gene05142 | MS.gene50174 | 0.805118 | 1.56E-49 | -1.69E-46 |
| MS.gene05142 | MS.gene52935 | 0.822629 | 2.14E-53 | -1.69E-46 |
| MS.gene05142 | MS.gene52937 | 0.87294 | 2.16E-67 | -1.69E-46 |
| MS.gene05142 | MS.gene71614 | 0.806011 | 1.01E-49 | -1.69E-46 |
| MS.gene05142 | MS.gene76146 | 0.820119 | 8.12E-53 | -1.69E-46 |
| MS.gene05142 | MS.gene85220 | 0.834514 | 2.87E-56 | -1.69E-46 |
| MS.gene05142 | MS.gene85568 | 0.84697 | 1.55E-59 | -1.69E-46 |
| MS.gene05142 | MS.gene86921 | 0.843134 | 1.69E-58 | -1.69E-46 |
| MS.gene05142 | MS.gene88914 | 0.818031 | 2.42E-52 | -1.69E-46 |
| MS.gene05142 | MS.gene93015 | 0.835625 | 1.51E-56 | -1.69E-46 |
| MS.gene05142 | MS.gene96692 | 0.843416 | 1.42E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05142.t1 | MTR_3g467420 | 95.434 | 219 | 10 | 0 | 2 | 220 | 6 | 224 | 9.15e-159 | 438 |
| MS.gene05142.t1 | MTR_3g467430 | 90.000 | 220 | 22 | 0 | 1 | 220 | 5 | 224 | 1.26e-148 | 412 |
| MS.gene05142.t1 | MTR_2g070180 | 61.429 | 210 | 81 | 0 | 6 | 215 | 5 | 214 | 2.15e-100 | 290 |
| MS.gene05142.t1 | MTR_2g070150 | 65.500 | 200 | 69 | 0 | 6 | 205 | 5 | 204 | 3.77e-100 | 289 |
| MS.gene05142.t1 | MTR_2g070140 | 64.039 | 203 | 73 | 0 | 18 | 220 | 1 | 203 | 1.88e-99 | 287 |
| MS.gene05142.t1 | MTR_2g070130 | 60.930 | 215 | 84 | 0 | 6 | 220 | 5 | 219 | 6.48e-99 | 286 |
| MS.gene05142.t1 | MTR_2g070210 | 62.212 | 217 | 80 | 1 | 6 | 220 | 5 | 221 | 2.77e-97 | 282 |
| MS.gene05142.t1 | MTR_2g070060 | 58.140 | 215 | 90 | 0 | 6 | 220 | 5 | 219 | 1.83e-95 | 278 |
| MS.gene05142.t1 | MTR_2g070200 | 62.673 | 217 | 79 | 2 | 6 | 220 | 5 | 221 | 2.41e-93 | 272 |
| MS.gene05142.t1 | MTR_2g070070 | 59.447 | 217 | 88 | 0 | 4 | 220 | 3 | 219 | 7.15e-92 | 269 |
| MS.gene05142.t1 | MTR_2g070120 | 57.075 | 212 | 91 | 0 | 6 | 217 | 5 | 216 | 1.80e-88 | 260 |
| MS.gene05142.t1 | MTR_8g056940 | 53.636 | 220 | 101 | 1 | 1 | 220 | 1 | 219 | 4.81e-88 | 259 |
| MS.gene05142.t1 | MTR_2g070110 | 57.143 | 217 | 92 | 1 | 4 | 220 | 3 | 218 | 8.49e-88 | 258 |
| MS.gene05142.t1 | MTR_4g124130 | 54.338 | 219 | 99 | 1 | 3 | 220 | 2 | 220 | 7.52e-86 | 253 |
| MS.gene05142.t1 | MTR_5g076900 | 50.000 | 222 | 108 | 3 | 1 | 220 | 1 | 221 | 1.27e-77 | 233 |
| MS.gene05142.t1 | MTR_0186s0030 | 54.930 | 142 | 63 | 1 | 1 | 142 | 1 | 141 | 3.76e-53 | 167 |
| MS.gene05142.t1 | MTR_8g061950 | 38.627 | 233 | 126 | 5 | 1 | 219 | 1 | 230 | 1.22e-47 | 157 |
| MS.gene05142.t1 | MTR_7g065720 | 43.269 | 208 | 112 | 6 | 6 | 209 | 8 | 213 | 1.92e-46 | 153 |
| MS.gene05142.t1 | MTR_8g087425 | 38.739 | 222 | 132 | 3 | 1 | 219 | 1 | 221 | 1.92e-45 | 150 |
| MS.gene05142.t1 | MTR_5g037380 | 40.000 | 220 | 123 | 4 | 1 | 211 | 1 | 220 | 3.08e-45 | 150 |
| MS.gene05142.t1 | MTR_7g065270 | 43.367 | 196 | 105 | 6 | 14 | 206 | 16 | 208 | 1.22e-44 | 149 |
| MS.gene05142.t1 | MTR_7g065660 | 39.423 | 208 | 121 | 4 | 5 | 209 | 7 | 212 | 1.94e-44 | 148 |
| MS.gene05142.t1 | MTR_1g090060 | 39.906 | 213 | 121 | 4 | 1 | 209 | 1 | 210 | 3.32e-44 | 147 |
| MS.gene05142.t1 | MTR_7g065740 | 39.336 | 211 | 122 | 4 | 3 | 209 | 5 | 213 | 1.04e-43 | 146 |
| MS.gene05142.t1 | MTR_3g099757 | 46.154 | 169 | 90 | 1 | 1 | 169 | 1 | 168 | 2.29e-43 | 145 |
| MS.gene05142.t1 | MTR_8g087410 | 41.379 | 203 | 116 | 2 | 1 | 201 | 2 | 203 | 7.06e-43 | 144 |
| MS.gene05142.t1 | MTR_4g019780 | 41.014 | 217 | 116 | 8 | 1 | 209 | 1 | 213 | 1.35e-42 | 143 |
| MS.gene05142.t1 | MTR_7g065290 | 41.063 | 207 | 117 | 5 | 14 | 217 | 16 | 220 | 2.51e-42 | 143 |
| MS.gene05142.t1 | MTR_7g065265 | 42.029 | 207 | 115 | 5 | 14 | 217 | 16 | 220 | 5.11e-42 | 142 |
| MS.gene05142.t1 | MTR_4g019790 | 38.095 | 210 | 125 | 4 | 3 | 209 | 5 | 212 | 8.00e-42 | 141 |
| MS.gene05142.t1 | MTR_7g065630 | 37.915 | 211 | 122 | 5 | 2 | 209 | 22 | 226 | 1.13e-41 | 141 |
| MS.gene05142.t1 | MTR_7g065230 | 41.667 | 204 | 110 | 6 | 6 | 207 | 8 | 204 | 1.77e-41 | 140 |
| MS.gene05142.t1 | MTR_4g059730 | 39.524 | 210 | 122 | 3 | 11 | 216 | 10 | 218 | 2.07e-41 | 140 |
| MS.gene05142.t1 | MTR_7g065590 | 40.201 | 199 | 114 | 5 | 14 | 209 | 16 | 212 | 2.66e-41 | 140 |
| MS.gene05142.t1 | MTR_7g065750 | 41.089 | 202 | 109 | 5 | 6 | 201 | 8 | 205 | 6.58e-41 | 139 |
| MS.gene05142.t1 | MTR_5g040430 | 34.450 | 209 | 129 | 3 | 4 | 205 | 6 | 213 | 9.54e-41 | 139 |
| MS.gene05142.t1 | MTR_1g090150 | 39.336 | 211 | 111 | 7 | 5 | 206 | 3 | 205 | 1.83e-40 | 138 |
| MS.gene05142.t1 | MTR_7g065710 | 38.049 | 205 | 122 | 4 | 6 | 209 | 5 | 205 | 1.09e-39 | 136 |
| MS.gene05142.t1 | MTR_7g065600 | 39.000 | 200 | 117 | 4 | 5 | 201 | 7 | 204 | 4.63e-39 | 134 |
| MS.gene05142.t1 | MTR_1g090100 | 38.571 | 210 | 124 | 3 | 5 | 210 | 2 | 210 | 5.07e-39 | 134 |
| MS.gene05142.t1 | MTR_7g065260 | 42.932 | 191 | 104 | 5 | 14 | 201 | 23 | 211 | 2.50e-38 | 132 |
| MS.gene05142.t1 | MTR_6g080440 | 37.313 | 201 | 118 | 5 | 19 | 213 | 17 | 215 | 4.56e-38 | 132 |
| MS.gene05142.t1 | MTR_1g090070 | 37.255 | 204 | 122 | 4 | 12 | 210 | 16 | 218 | 1.42e-37 | 130 |
| MS.gene05142.t1 | MTR_7g065700 | 40.385 | 208 | 119 | 5 | 3 | 209 | 5 | 208 | 1.43e-37 | 130 |
| MS.gene05142.t1 | MTR_7g065680 | 39.130 | 207 | 117 | 5 | 6 | 209 | 8 | 208 | 3.06e-37 | 129 |
| MS.gene05142.t1 | MTR_1g090090 | 38.967 | 213 | 123 | 5 | 1 | 209 | 1 | 210 | 7.29e-37 | 129 |
| MS.gene05142.t1 | MTR_1g115195 | 38.424 | 203 | 119 | 5 | 4 | 201 | 2 | 203 | 3.76e-35 | 124 |
| MS.gene05142.t1 | MTR_7g065640 | 35.266 | 207 | 113 | 5 | 5 | 209 | 7 | 194 | 3.65e-34 | 121 |
| MS.gene05142.t1 | MTR_2g072120 | 58.824 | 68 | 28 | 0 | 48 | 115 | 1 | 68 | 6.66e-25 | 93.6 |
| MS.gene05142.t1 | MTR_7g100320 | 29.592 | 196 | 125 | 8 | 16 | 206 | 37 | 224 | 3.45e-15 | 72.4 |
| MS.gene05142.t1 | MTR_4g043640 | 57.143 | 42 | 18 | 0 | 149 | 190 | 44 | 85 | 1.17e-12 | 62.0 |
| MS.gene05142.t1 | MTR_1g067180 | 31.852 | 135 | 81 | 7 | 55 | 186 | 79 | 205 | 2.13e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05142.t1 | AT1G17180 | 64.186 | 215 | 77 | 0 | 6 | 220 | 5 | 219 | 3.27e-102 | 295 |
| MS.gene05142.t1 | AT1G78370 | 61.611 | 211 | 81 | 0 | 7 | 217 | 6 | 216 | 5.57e-102 | 294 |
| MS.gene05142.t1 | AT1G78380 | 61.033 | 213 | 83 | 0 | 4 | 216 | 3 | 215 | 7.36e-100 | 289 |
| MS.gene05142.t1 | AT1G17170 | 62.791 | 215 | 79 | 1 | 6 | 220 | 5 | 218 | 1.04e-97 | 283 |
| MS.gene05142.t1 | AT1G78360 | 59.633 | 218 | 87 | 1 | 4 | 220 | 3 | 220 | 1.70e-95 | 278 |
| MS.gene05142.t1 | AT1G78340 | 57.143 | 210 | 90 | 0 | 6 | 215 | 5 | 214 | 4.38e-92 | 269 |
| MS.gene05142.t1 | AT1G53680 | 56.682 | 217 | 92 | 2 | 6 | 220 | 8 | 224 | 4.09e-89 | 262 |
| MS.gene05142.t1 | AT1G78320 | 59.804 | 204 | 82 | 0 | 3 | 206 | 2 | 205 | 5.47e-87 | 256 |
| MS.gene05142.t1 | AT1G17190 | 52.752 | 218 | 103 | 0 | 1 | 218 | 1 | 218 | 1.22e-82 | 245 |
| MS.gene05142.t1 | AT3G43800 | 51.131 | 221 | 104 | 3 | 1 | 217 | 1 | 221 | 7.95e-79 | 236 |
| MS.gene05142.t1 | AT1G78320 | 51.961 | 204 | 71 | 1 | 3 | 206 | 2 | 178 | 1.77e-69 | 211 |
| MS.gene05142.t1 | AT1G10360 | 42.202 | 218 | 118 | 4 | 1 | 210 | 1 | 218 | 1.86e-50 | 164 |
| MS.gene05142.t1 | AT2G29420 | 42.424 | 198 | 111 | 3 | 6 | 201 | 10 | 206 | 4.67e-50 | 162 |
| MS.gene05142.t1 | AT1G10370 | 40.000 | 225 | 113 | 5 | 1 | 210 | 1 | 218 | 1.42e-45 | 151 |
| MS.gene05142.t1 | AT3G09270 | 38.647 | 207 | 124 | 2 | 3 | 206 | 4 | 210 | 1.08e-44 | 149 |
| MS.gene05142.t1 | AT1G59700 | 39.910 | 223 | 119 | 5 | 6 | 216 | 7 | 226 | 1.49e-44 | 149 |
| MS.gene05142.t1 | AT1G74590 | 35.586 | 222 | 127 | 5 | 6 | 216 | 8 | 224 | 7.42e-44 | 147 |
| MS.gene05142.t1 | AT5G62480 | 37.383 | 214 | 123 | 4 | 2 | 205 | 5 | 217 | 7.82e-43 | 144 |
| MS.gene05142.t1 | AT1G69930 | 37.736 | 212 | 125 | 3 | 6 | 210 | 13 | 224 | 1.94e-41 | 141 |
| MS.gene05142.t1 | AT1G27130 | 35.586 | 222 | 133 | 5 | 1 | 212 | 1 | 222 | 6.57e-41 | 139 |
| MS.gene05142.t1 | AT2G29490 | 37.379 | 206 | 125 | 2 | 3 | 205 | 5 | 209 | 1.45e-40 | 138 |
| MS.gene05142.t1 | AT2G29440 | 38.191 | 199 | 119 | 2 | 6 | 201 | 7 | 204 | 5.14e-40 | 137 |
| MS.gene05142.t1 | AT1G59670 | 38.182 | 220 | 127 | 5 | 6 | 216 | 7 | 226 | 6.58e-40 | 137 |
| MS.gene05142.t1 | AT2G29480 | 37.931 | 203 | 120 | 4 | 3 | 201 | 5 | 205 | 1.27e-39 | 136 |
| MS.gene05142.t1 | AT2G29450 | 38.009 | 221 | 124 | 6 | 1 | 213 | 1 | 216 | 3.79e-39 | 134 |
| MS.gene05142.t1 | AT1G27140 | 33.632 | 223 | 137 | 5 | 1 | 212 | 1 | 223 | 5.42e-38 | 132 |
| MS.gene05142.t1 | AT2G29470 | 36.538 | 208 | 125 | 5 | 3 | 205 | 5 | 210 | 2.43e-36 | 127 |
| MS.gene05142.t1 | AT2G29460 | 36.453 | 203 | 123 | 4 | 3 | 201 | 5 | 205 | 1.52e-34 | 123 |
| MS.gene05142.t1 | AT1G69920 | 30.415 | 217 | 138 | 5 | 5 | 210 | 34 | 248 | 4.95e-30 | 112 |
| MS.gene05142.t1 | AT5G62480 | 29.439 | 214 | 114 | 5 | 2 | 205 | 5 | 191 | 6.68e-24 | 95.1 |
| MS.gene05142.t1 | AT5G62480 | 29.439 | 214 | 113 | 5 | 2 | 205 | 5 | 190 | 7.73e-24 | 94.7 |
| MS.gene05142.t1 | AT5G02790 | 34.810 | 158 | 86 | 8 | 55 | 206 | 80 | 226 | 1.39e-12 | 65.1 |
Find 43 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGACAAACCATATTT+TGG | 0.218729 | 3.4:-64457553 | MS.gene05142:CDS |
| AATTCATCTCCACCAAAATA+TGG | 0.270774 | 3.4:+64457541 | None:intergenic |
| AGCGATCACAAGCCAAGTTT+TGG | 0.291618 | 3.4:-64458490 | MS.gene05142:CDS |
| CCCCACCCTCACAAAATCTA+TGG | 0.311159 | 3.4:-64457370 | MS.gene05142:CDS |
| TTTCCATTATGAATCAAAAT+CGG | 0.338158 | 3.4:+64458599 | None:intergenic |
| GTGTCCTAAGCTTGTGGCTT+GGG | 0.342320 | 3.4:-64457434 | MS.gene05142:CDS |
| TTGGCTTGTGATCGCTTGTA+AGG | 0.379033 | 3.4:+64458497 | None:intergenic |
| CTCTTATTCCATTCACAAGT+TGG | 0.382881 | 3.4:-64457501 | MS.gene05142:CDS |
| ATATTAAGTGATTCACATAT+AGG | 0.400119 | 3.4:+64458575 | None:intergenic |
| TTCCATTATGAATCAAAATC+GGG | 0.401812 | 3.4:+64458600 | None:intergenic |
| GAATGCTTGAAGACTTTAGA+AGG | 0.402622 | 3.4:-64457580 | MS.gene05142:CDS |
| TTGGCCCAAGCCACAAGCTT+AGG | 0.419595 | 3.4:+64457430 | None:intergenic |
| AAGACTTTAGAAGGTGAGCT+TGG | 0.438837 | 3.4:-64457571 | MS.gene05142:CDS |
| TTTACACGTACGAGATATAT+GGG | 0.461663 | 3.4:-64457477 | MS.gene05142:CDS |
| AGCTTGTGGCTTGGGCCAAA+AGG | 0.465671 | 3.4:-64457426 | MS.gene05142:CDS |
| TTGAGTACATTGATGAAGCT+TGG | 0.466341 | 3.4:-64458550 | MS.gene05142:CDS |
| GTGTAAAACCAACTTGTGAA+TGG | 0.467431 | 3.4:+64457493 | None:intergenic |
| GATATACAACATTGGAAAGA+AGG | 0.483460 | 3.4:-64457653 | MS.gene05142:CDS |
| AATGTAATCTCCCCAAAACT+TGG | 0.487995 | 3.4:+64458478 | None:intergenic |
| ACTCTCATTCCATATGAGCT+TGG | 0.497119 | 3.4:+64458728 | None:intergenic |
| AAGGGTGAAGAACAAGAAGA+TGG | 0.504753 | 3.4:-64457619 | MS.gene05142:CDS |
| AAAATTGCATTGGAAGAGAA+GGG | 0.514397 | 3.4:-64458704 | MS.gene05142:CDS |
| AAGAAGGTATGGACAGGAAA+GGG | 0.519335 | 3.4:-64457637 | MS.gene05142:CDS |
| AATGAGAGTGAAAATTGCAT+TGG | 0.532849 | 3.4:-64458714 | MS.gene05142:CDS |
| ACAACATTGGAAAGAAGGTA+TGG | 0.535319 | 3.4:-64457648 | MS.gene05142:CDS |
| AGTGTCCTAAGCTTGTGGCT+TGG | 0.547472 | 3.4:-64457435 | MS.gene05142:CDS |
| GAAAATTGCATTGGAAGAGA+AGG | 0.550919 | 3.4:-64458705 | MS.gene05142:CDS |
| GGCTTGGGCCAAAAGGTGTA+TGG | 0.551448 | 3.4:-64457419 | MS.gene05142:CDS |
| ATTGGAAAGAAGGTATGGAC+AGG | 0.560806 | 3.4:-64457643 | MS.gene05142:CDS |
| TGATCGCTTGTAAGGGTCAG+AGG | 0.571746 | 3.4:+64458505 | None:intergenic |
| ATGCAATATAAACAGAAGCA+TGG | 0.576909 | 3.4:-64457340 | MS.gene05142:CDS |
| TGAATTTGGGTATGTTGATG+TGG | 0.584095 | 3.4:-64457524 | MS.gene05142:CDS |
| AATGAGTCAATATATGGCTG+AGG | 0.585478 | 3.4:-64458777 | None:intergenic |
| GGGTCAGAGGGCAAAAGAGA+AGG | 0.589806 | 3.4:+64458518 | None:intergenic |
| TGGCTTGTGATCGCTTGTAA+GGG | 0.589840 | 3.4:+64458498 | None:intergenic |
| GAAAGAGTGTCCTAAGCTTG+TGG | 0.598040 | 3.4:-64457440 | MS.gene05142:CDS |
| ATGAGTCAATATATGGCTGA+GGG | 0.621155 | 3.4:-64458776 | None:intergenic |
| AAAGAAGGTATGGACAGGAA+AGG | 0.623553 | 3.4:-64457638 | MS.gene05142:CDS |
| TTGATGTAGATATACAACAT+TGG | 0.625837 | 3.4:-64457661 | MS.gene05142:intron |
| GATCGCTTGTAAGGGTCAGA+GGG | 0.649504 | 3.4:+64458506 | None:intergenic |
| AAATTGCATTGGAAGAGAAG+GGG | 0.654003 | 3.4:-64458703 | MS.gene05142:CDS |
| AATTGCATTGGAAGAGAAGG+GGG | 0.673032 | 3.4:-64458702 | MS.gene05142:CDS |
| ATATATGGCTGAGGGCAATG+TGG | 0.718578 | 3.4:-64458768 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTAAAAAAATTATATGTT+TGG | + | chr3.4:64458370-64458389 | None:intergenic | 10.0% |
| !!! | TTTAATAGATAGATTTTTAT+AGG | - | chr3.4:64457780-64457799 | MS.gene05142:intron | 10.0% |
| !!! | AAATATTGGTAGTTTAATAA+GGG | - | chr3.4:64457804-64457823 | MS.gene05142:intron | 15.0% |
| !! | ATATTAAGTGATTCACATAT+AGG | + | chr3.4:64457522-64457541 | None:intergenic | 20.0% |
| !! | CAAATATGCTACAATATTTA+TGG | + | chr3.4:64458208-64458227 | None:intergenic | 20.0% |
| !! | CATAAATATTGTAGCATATT+TGG | - | chr3.4:64458206-64458225 | MS.gene05142:intron | 20.0% |
| !! | TATCTACTTAAGCATTAATA+TGG | + | chr3.4:64458095-64458114 | None:intergenic | 20.0% |
| !! | TTTCCATTATGAATCAAAAT+CGG | + | chr3.4:64457498-64457517 | None:intergenic | 20.0% |
| !!! | AATATTGGTAGTTTAATAAG+GGG | - | chr3.4:64457805-64457824 | MS.gene05142:intron | 20.0% |
| !!! | AGATTTTTATAGGCAAATAT+TGG | - | chr3.4:64457790-64457809 | MS.gene05142:intron | 20.0% |
| !!! | CAAATATTGGTAGTTTAATA+AGG | - | chr3.4:64457803-64457822 | MS.gene05142:intron | 20.0% |
| !!! | TCAAGTCTTGATTATTTTTT+TGG | - | chr3.4:64457950-64457969 | MS.gene05142:intron | 20.0% |
| !!! | TTTTTAAAGAGTTGATTCAT+AGG | - | chr3.4:64458381-64458400 | MS.gene05142:intron | 20.0% |
| ! | CAATTTACAATTTACATGAG+AGG | - | chr3.4:64458298-64458317 | MS.gene05142:intron | 25.0% |
| ! | GATATCATGGTCTAATTTAT+TGG | - | chr3.4:64458403-64458422 | MS.gene05142:intron | 25.0% |
| ! | TTCATAAAGATTGTCTATGA+AGG | + | chr3.4:64457927-64457946 | None:intergenic | 25.0% |
| ! | TTCCATTATGAATCAAAATC+GGG | + | chr3.4:64457497-64457516 | None:intergenic | 25.0% |
| ! | TTGATGTAGATATACAACAT+TGG | - | chr3.4:64458433-64458452 | MS.gene05142:intron | 25.0% |
| !! | TATGTGTGTTATTATGTTCA+AGG | - | chr3.4:64457666-64457685 | MS.gene05142:intron | 25.0% |
| !!! | GTCTTGATTATTTTTTTGGT+TGG | - | chr3.4:64457954-64457973 | MS.gene05142:intron | 25.0% |
| !!! | TGACCTATTTTCTTTTTCAA+CGG | + | chr3.4:64458137-64458156 | None:intergenic | 25.0% |
| AAAATTGCATTGGAAGAGAA+GGG | - | chr3.4:64457390-64457409 | MS.gene05142:CDS | 30.0% | |
| AATGAGAGTGAAAATTGCAT+TGG | - | chr3.4:64457380-64457399 | MS.gene05142:CDS | 30.0% | |
| AATTCATCTCCACCAAAATA+TGG | + | chr3.4:64458556-64458575 | None:intergenic | 30.0% | |
| ATGCAATATAAACAGAAGCA+TGG | - | chr3.4:64458754-64458773 | MS.gene05142:CDS | 30.0% | |
| ATTGTAAATTGTTTGCCACT+TGG | + | chr3.4:64458289-64458308 | None:intergenic | 30.0% | |
| CTTCCGTTGAAAAAGAAAAT+AGG | - | chr3.4:64458131-64458150 | MS.gene05142:intron | 30.0% | |
| GAAAAAAATGTAGTTGAGAG+TGG | + | chr3.4:64458070-64458089 | None:intergenic | 30.0% | |
| GATATACAACATTGGAAAGA+AGG | - | chr3.4:64458441-64458460 | MS.gene05142:intron | 30.0% | |
| TAGTGATGTCTATAAAGCTT+TGG | + | chr3.4:64457711-64457730 | None:intergenic | 30.0% | |
| TTTACACGTACGAGATATAT+GGG | - | chr3.4:64458617-64458636 | MS.gene05142:CDS | 30.0% | |
| ! | AGTTGATTCATAGGATATCA+TGG | - | chr3.4:64458390-64458409 | MS.gene05142:intron | 30.0% |
| ! | CAAAACCATAGATTTTGTGA+GGG | + | chr3.4:64458732-64458751 | None:intergenic | 30.0% |
| ! | TTGTAAATTGTTTGCCACTT+GGG | + | chr3.4:64458288-64458307 | None:intergenic | 30.0% |
| ! | TTTTACACGTACGAGATATA+TGG | - | chr3.4:64458616-64458635 | MS.gene05142:CDS | 30.0% |
| !! | ATTTTGGTGGAGATGAATTT+GGG | - | chr3.4:64458557-64458576 | MS.gene05142:CDS | 30.0% |
| !!! | ATCCCGATTTTGATTCATAA+TGG | - | chr3.4:64457492-64457511 | MS.gene05142:CDS | 30.0% |
| !!! | ATGTGGTTTTGTTGGATTTT+TGG | - | chr3.4:64457343-64457362 | MS.gene05142:CDS | 30.0% |
| !!! | TATTTTGGTGGAGATGAATT+TGG | - | chr3.4:64458556-64458575 | MS.gene05142:CDS | 30.0% |
| AAATTGCATTGGAAGAGAAG+GGG | - | chr3.4:64457391-64457410 | MS.gene05142:CDS | 35.0% | |
| AATGTAATCTCCCCAAAACT+TGG | + | chr3.4:64457619-64457638 | None:intergenic | 35.0% | |
| ACAACATTGGAAAGAAGGTA+TGG | - | chr3.4:64458446-64458465 | MS.gene05142:intron | 35.0% | |
| ATGATCGATCGTTAAGAATG+AGG | + | chr3.4:64457873-64457892 | None:intergenic | 35.0% | |
| CAAAATTAGTCCGACAAAGA+TGG | + | chr3.4:64458005-64458024 | None:intergenic | 35.0% | |
| CTCTTATTCCATTCACAAGT+TGG | - | chr3.4:64458593-64458612 | MS.gene05142:CDS | 35.0% | |
| GAAAATTGCATTGGAAGAGA+AGG | - | chr3.4:64457389-64457408 | MS.gene05142:CDS | 35.0% | |
| GAATGCTTGAAGACTTTAGA+AGG | - | chr3.4:64458514-64458533 | MS.gene05142:CDS | 35.0% | |
| GTGTAAAACCAACTTGTGAA+TGG | + | chr3.4:64458604-64458623 | None:intergenic | 35.0% | |
| TGAATTTGGGTATGTTGATG+TGG | - | chr3.4:64458570-64458589 | MS.gene05142:CDS | 35.0% | |
| TTGAGTACATTGATGAAGCT+TGG | - | chr3.4:64457544-64457563 | MS.gene05142:CDS | 35.0% | |
| ! | ATCGGGATCATTTTGTAAAC+CGG | + | chr3.4:64457480-64457499 | None:intergenic | 35.0% |
| ! | CATCTTTGTCGGACTAATTT+TGG | - | chr3.4:64458003-64458022 | MS.gene05142:intron | 35.0% |
| ! | CTCTTTTTCCATACACCTTT+TGG | + | chr3.4:64458686-64458705 | None:intergenic | 35.0% |
| ! | CTTGGAGACAAACCATATTT+TGG | - | chr3.4:64458541-64458560 | MS.gene05142:CDS | 35.0% |
| ! | GCAAAACCATAGATTTTGTG+AGG | + | chr3.4:64458733-64458752 | None:intergenic | 35.0% |
| !!! | TTTTTGGTTGGTCACGTTAT+TGG | - | chr3.4:64457966-64457985 | MS.gene05142:intron | 35.0% |
| AACTGGAAACCCACTAAAAG+TGG | + | chr3.4:64458046-64458065 | None:intergenic | 40.0% | |
| AAGGGTGAAGAACAAGAAGA+TGG | - | chr3.4:64458475-64458494 | MS.gene05142:CDS | 40.0% | |
| AATTAGTCCGACAAAGATGG+TGG | + | chr3.4:64458002-64458021 | None:intergenic | 40.0% | |
| AATTGCATTGGAAGAGAAGG+GGG | - | chr3.4:64457392-64457411 | MS.gene05142:CDS | 40.0% | |
| ACTCTCATTCCATATGAGCT+TGG | + | chr3.4:64457369-64457388 | None:intergenic | 40.0% | |
| ATGTAGTTGAGAGTGGAAAC+TGG | + | chr3.4:64458063-64458082 | None:intergenic | 40.0% | |
| GATCGTTAAGAATGAGGTCT+AGG | + | chr3.4:64457867-64457886 | None:intergenic | 40.0% | |
| GTGTATGGAAAAAGAGAGTG+TGG | - | chr3.4:64458690-64458709 | MS.gene05142:CDS | 40.0% | |
| TATATCCATTCAAGCCCAAG+TGG | - | chr3.4:64458271-64458290 | MS.gene05142:intron | 40.0% | |
| ! | AAAGAAGGTATGGACAGGAA+AGG | - | chr3.4:64458456-64458475 | MS.gene05142:intron | 40.0% |
| ! | AACCATAGATTTTGTGAGGG+TGG | + | chr3.4:64458729-64458748 | None:intergenic | 40.0% |
| ! | AAGAAGGTATGGACAGGAAA+GGG | - | chr3.4:64458457-64458476 | MS.gene05142:intron | 40.0% |
| ! | AAGACTTTAGAAGGTGAGCT+TGG | - | chr3.4:64458523-64458542 | MS.gene05142:CDS | 40.0% |
| ! | ACCATAGATTTTGTGAGGGT+GGG | + | chr3.4:64458728-64458747 | None:intergenic | 40.0% |
| ! | AGGGCAAAAGAGAAGGTTTT+TGG | + | chr3.4:64457572-64457591 | None:intergenic | 40.0% |
| ! | ATTGGAAAGAAGGTATGGAC+AGG | - | chr3.4:64458451-64458470 | MS.gene05142:intron | 40.0% |
| ! | GAAGTATCCACCATCTTTGT+CGG | - | chr3.4:64457992-64458011 | MS.gene05142:intron | 40.0% |
| ! | TCGGGATCATTTTGTAAACC+GGG | + | chr3.4:64457479-64457498 | None:intergenic | 40.0% |
| !! | CTAATTTTGGCCGGAGATTA+TGG | - | chr3.4:64458016-64458035 | MS.gene05142:intron | 40.0% |
| !! | GGAGACAAACCATATTTTGG+TGG | - | chr3.4:64458544-64458563 | MS.gene05142:CDS | 40.0% |
| !! | TCTGCTTTTAGAGATGAACC+CGG | - | chr3.4:64457458-64457477 | MS.gene05142:CDS | 40.0% |
| !! | TTTGTCGGACTAATTTTGGC+CGG | - | chr3.4:64458007-64458026 | MS.gene05142:intron | 40.0% |
| !!! | GATTTTTGGCCAAGCTCATA+TGG | - | chr3.4:64457357-64457376 | MS.gene05142:CDS | 40.0% |
| !!! | TATGGCTAGCCACTTTTAGT+GGG | - | chr3.4:64458034-64458053 | MS.gene05142:intron | 40.0% |
| !!! | TTATGGCTAGCCACTTTTAG+TGG | - | chr3.4:64458033-64458052 | MS.gene05142:intron | 40.0% |
| ! | AAGTGGCTAGCCATAATCTC+CGG | + | chr3.4:64458029-64458048 | None:intergenic | 45.0% |
| ! | AGCGATCACAAGCCAAGTTT+TGG | - | chr3.4:64457604-64457623 | MS.gene05142:CDS | 45.0% |
| ! | CCATAGATTTTGTGAGGGTG+GGG | + | chr3.4:64458727-64458746 | None:intergenic | 45.0% |
| ! | CGATCACAAGCCAAGTTTTG+GGG | - | chr3.4:64457606-64457625 | MS.gene05142:CDS | 45.0% |
| ! | GCGATCACAAGCCAAGTTTT+GGG | - | chr3.4:64457605-64457624 | MS.gene05142:CDS | 45.0% |
| ! | TGGCTTGTGATCGCTTGTAA+GGG | + | chr3.4:64457599-64457618 | None:intergenic | 45.0% |
| !! | GAAAGAGTGTCCTAAGCTTG+TGG | - | chr3.4:64458654-64458673 | MS.gene05142:CDS | 45.0% |
| !! | TTGGCTTGTGATCGCTTGTA+AGG | + | chr3.4:64457600-64457619 | None:intergenic | 45.0% |
| !!! | TGAGGGCAATGTGGTTTTGT+TGG | - | chr3.4:64457335-64457354 | MS.gene05142:CDS | 45.0% |
| AGTGTCCTAAGCTTGTGGCT+TGG | - | chr3.4:64458659-64458678 | MS.gene05142:CDS | 50.0% | |
| CCCCACCCTCACAAAATCTA+TGG | - | chr3.4:64458724-64458743 | MS.gene05142:CDS | 50.0% | |
| GATCGCTTGTAAGGGTCAGA+GGG | + | chr3.4:64457591-64457610 | None:intergenic | 50.0% | |
| GTGTCCTAAGCTTGTGGCTT+GGG | - | chr3.4:64458660-64458679 | MS.gene05142:CDS | 50.0% | |
| TGATCGCTTGTAAGGGTCAG+AGG | + | chr3.4:64457592-64457611 | None:intergenic | 50.0% | |
| ! | GTTTGCCACTTGGGCTTGAA+TGG | + | chr3.4:64458279-64458298 | None:intergenic | 50.0% |
| GGGTCAGAGGGCAAAAGAGA+AGG | + | chr3.4:64457579-64457598 | None:intergenic | 55.0% | |
| TTGGCCCAAGCCACAAGCTT+AGG | + | chr3.4:64458667-64458686 | None:intergenic | 55.0% | |
| ! | AGCTTGTGGCTTGGGCCAAA+AGG | - | chr3.4:64458668-64458687 | MS.gene05142:CDS | 55.0% |
| ! | GGCTTGGGCCAAAAGGTGTA+TGG | - | chr3.4:64458675-64458694 | MS.gene05142:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 64457330 | 64458786 | 64457330 | ID=MS.gene05142 |
| chr3.4 | mRNA | 64457330 | 64458786 | 64457330 | ID=MS.gene05142.t1;Parent=MS.gene05142 |
| chr3.4 | exon | 64458469 | 64458786 | 64458469 | ID=MS.gene05142.t1.exon1;Parent=MS.gene05142.t1 |
| chr3.4 | CDS | 64458469 | 64458786 | 64458469 | ID=cds.MS.gene05142.t1;Parent=MS.gene05142.t1 |
| chr3.4 | exon | 64457330 | 64457674 | 64457330 | ID=MS.gene05142.t1.exon2;Parent=MS.gene05142.t1 |
| chr3.4 | CDS | 64457330 | 64457674 | 64457330 | ID=cds.MS.gene05142.t1;Parent=MS.gene05142.t1 |
| Gene Sequence |
| Protein sequence |
