AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53261

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53261.t1 MTR_8g087430 99.015 203 2 0 1 203 1 203 5.05e-147 407
MS.gene53261.t1 MTR_7g056337 72.772 202 55 0 1 202 1 202 1.09e-104 300
MS.gene53261.t1 MTR_4g075550 36.620 213 117 8 2 201 47 254 5.31e-27 103
MS.gene53261.t1 MTR_8g012595 35.514 214 121 6 2 201 47 257 1.01e-26 103
MS.gene53261.t1 MTR_3g113920 33.645 214 122 7 2 201 47 254 7.13e-26 100
MS.gene53261.t1 MTR_8g012605 35.465 172 103 4 2 169 47 214 2.91e-25 99.4
MS.gene53261.t1 MTR_8g012615 34.239 184 105 6 31 201 9 189 1.83e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53261.t1 AT1G29330 81.773 203 37 0 1 203 1 203 8.51e-125 351
MS.gene53261.t1 AT3G25040 71.429 203 58 0 1 203 1 203 2.48e-107 307
MS.gene53261.t1 AT1G29330 84.397 141 22 0 63 203 1 141 1.45e-85 249
MS.gene53261.t1 AT3G25160 34.272 213 124 4 2 201 48 257 4.21e-28 107
MS.gene53261.t1 AT1G19970 35.047 214 119 8 2 201 47 254 8.38e-26 100
MS.gene53261.t1 AT4G38790 34.112 214 121 8 2 201 48 255 3.63e-25 99.4
MS.gene53261.t1 AT2G21190 32.710 214 124 8 2 201 44 251 4.25e-25 99.0
MS.gene53261.t1 AT1G75760 33.178 214 123 7 2 201 47 254 6.76e-25 98.2
MS.gene53261.t1 AT1G75760 34.112 214 121 8 2 201 47 254 8.51e-25 98.2
MS.gene53261.t1 AT1G19970 35.028 177 104 6 2 173 47 217 2.20e-22 91.3
MS.gene53261.t1 AT1G75760 33.714 175 105 6 2 171 47 215 5.25e-21 87.4

Find 35 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTCAATTTCAGATCTTC+TGG 0.214649 4.1:+13278396 MS.gene53261:intron
GGGCAATATGTATTCTTTCT+TGG 0.265304 4.1:+13278503 MS.gene53261:CDS
TGTGTTCTTAGCACGTTATT+TGG 0.272313 4.1:+13275369 MS.gene53261:CDS
TGTTGCTGCTAGCTTTCTTC+TGG 0.281140 4.1:+13275543 MS.gene53261:CDS
GGCAATATGTATTCTTTCTT+GGG 0.306579 4.1:+13278504 MS.gene53261:CDS
TCTTCTACTATTACTTCATC+AGG 0.375089 4.1:+13279090 MS.gene53261:CDS
GTGGATTATACATTCTCAAT+TGG 0.399809 4.1:+13278894 MS.gene53261:CDS
TACGCTACCAAATCATGTTC+AGG 0.407698 4.1:+13275227 MS.gene53261:CDS
ATTGTGACCTGAACATGATT+TGG 0.429861 4.1:-13275234 None:intergenic
CTTCATAAACGTATTATAAA+CGG 0.434373 4.1:-13275413 None:intergenic
CTAGTTCGTTGGCTATTGTT+TGG 0.468529 4.1:+13275449 MS.gene53261:CDS
ACGTTATTTGGATCTGTTCA+CGG 0.477076 4.1:+13275381 MS.gene53261:CDS
ATGTCAATTTCAGATCTTCT+GGG 0.481234 4.1:+13278397 MS.gene53261:intron
GGAAATGTTGATAATTTGAC+TGG 0.481668 4.1:+13278482 MS.gene53261:CDS
GGCGAGTCTGAAGATGTTCA+TGG 0.501045 4.1:-13275156 None:intergenic
TTCGTTGCAACAAAACCAGC+TGG 0.501585 4.1:-13278457 None:intergenic
GTGCATGCGTGTTCATCCTA+TGG 0.502901 4.1:+13275471 MS.gene53261:CDS
TCATAGCTTCTCTTAACCAT+AGG 0.504328 4.1:-13275487 None:intergenic
CTGAACAACACCTGAGACAC+AGG 0.506289 4.1:-13279048 None:intergenic
CTGAGCCACGTTTCACTCGA+TGG 0.510830 4.1:+13278933 MS.gene53261:CDS
AGTTGCAATACTGCCCCAGC+TGG 0.517340 4.1:+13278442 MS.gene53261:CDS
AGTCTTCATTGCTAGTTCGT+TGG 0.524045 4.1:+13275438 MS.gene53261:CDS
CCACGTTTCACTCGATGGAT+AGG 0.526685 4.1:+13278938 MS.gene53261:CDS
GATAATGACGAAAAGTATCA+AGG 0.541133 4.1:-13275516 None:intergenic
TTAAATTGCAGTGCGTATCG+TGG 0.598999 4.1:+13278875 MS.gene53261:intron
CGAGAAATTCACCTTCCTTG+AGG 0.604093 4.1:+13275579 MS.gene53261:CDS
TCGTTGCAACAAAACCAGCT+GGG 0.607236 4.1:-13278456 None:intergenic
ATGAACATCTTCAGACTCGC+CGG 0.613512 4.1:+13275158 MS.gene53261:CDS
ATAGAAAGGGACCTCAAGGA+AGG 0.624693 4.1:-13275590 None:intergenic
GAGGAGATGAGTCATGTCAC+CGG 0.626170 4.1:-13275177 None:intergenic
CCTATCCATCGAGTGAAACG+TGG 0.656144 4.1:-13278938 None:intergenic
CGTTGCAACAAAACCAGCTG+GGG 0.659984 4.1:-13278455 None:intergenic
GGAGGAGAACGAGAATGCTG+AGG 0.684833 4.1:-13275196 None:intergenic
AACGTGGCTCAGTCAAATAG+CGG 0.705507 4.1:-13278922 None:intergenic
GAAATGTTGATAATTTGACT+GGG 0.715465 4.1:+13278483 MS.gene53261:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTAGTATAATTTGAA+AGG + chr4.1:13277928-13277947 MS.gene53261:intron 10.0%
!! AATAAGGCTATAAAATAAAA+AGG + chr4.1:13276229-13276248 MS.gene53261:intron 15.0%
!! GATAATTATTAAAAGAAACA+TGG + chr4.1:13276318-13276337 MS.gene53261:intron 15.0%
!!! ATTTGAAAATGTTGTTTATT+TGG + chr4.1:13278685-13278704 MS.gene53261:intron 15.0%
!!! TTGATAATTGATATATGTAA+TGG + chr4.1:13278654-13278673 MS.gene53261:intron 15.0%
!! ATAAGATTCATAGATAGAAA+GGG - chr4.1:13275606-13275625 None:intergenic 20.0%
!! ATTACAGTATGAGTAAAATA+AGG + chr4.1:13276213-13276232 MS.gene53261:intron 20.0%
!! CACTGCAATTTAAAAATAAA+TGG - chr4.1:13278868-13278887 None:intergenic 20.0%
!! CTTCATAAACGTATTATAAA+CGG - chr4.1:13275416-13275435 None:intergenic 20.0%
!! GAAACTAACAAAAAAAAACA+GGG - chr4.1:13277484-13277503 None:intergenic 20.0%
!! GCATTAAATAGTTAGAAAAA+GGG - chr4.1:13276415-13276434 None:intergenic 20.0%
!! TGAAACTAACAAAAAAAAAC+AGG - chr4.1:13277485-13277504 None:intergenic 20.0%
!! TGAACAAATCAACTTAATTT+AGG - chr4.1:13277024-13277043 None:intergenic 20.0%
!! TGAATAAATAAAACAGAAAC+TGG - chr4.1:13276689-13276708 None:intergenic 20.0%
!! TGCATTAAATAGTTAGAAAA+AGG - chr4.1:13276416-13276435 None:intergenic 20.0%
!! TTAAAAAAATTCAAACAGGT+CGG - chr4.1:13276626-13276645 None:intergenic 20.0%
!! TTTAGTATAATTTGAAAGGA+TGG + chr4.1:13277932-13277951 MS.gene53261:intron 20.0%
!!! AGAACAAGTTTTTAATCAAA+TGG + chr4.1:13277903-13277922 MS.gene53261:intron 20.0%
!!! ATTTTTTTCTCTTCAATTTC+AGG + chr4.1:13275313-13275332 MS.gene53261:intron 20.0%
!!! GTTTTTTTTTGTTAGTTTCA+TGG + chr4.1:13277485-13277504 MS.gene53261:intron 20.0%
!!! TTAGTTCTATAAACCAATTT+TGG - chr4.1:13277458-13277477 None:intergenic 20.0%
!!! TTTTTATCGCAATTTCAAAA+GGG - chr4.1:13277382-13277401 None:intergenic 20.0%
!!! TTTTTTATCGCAATTTCAAA+AGG - chr4.1:13277383-13277402 None:intergenic 20.0%
! AAGATTTGAAAACAATTGAC+AGG - chr4.1:13278231-13278250 None:intergenic 25.0%
! AGAGAAAATACATTTCTTAG+AGG - chr4.1:13278632-13278651 None:intergenic 25.0%
! ATAAAGAAATGACTTTACAG+TGG - chr4.1:13277752-13277771 None:intergenic 25.0%
! ATAAAGCAGTCAAAACATAA+AGG - chr4.1:13278571-13278590 None:intergenic 25.0%
! ATATCTTTGTCCAAAATGTT+TGG - chr4.1:13277981-13278000 None:intergenic 25.0%
! ATATGATGATATGATCTCTT+GGG + chr4.1:13276086-13276105 MS.gene53261:intron 25.0%
! ATGAAATGAAACTTATCCAT+AGG - chr4.1:13277605-13277624 None:intergenic 25.0%
! ATGGTATGAAAGTAAACAAT+TGG - chr4.1:13275851-13275870 None:intergenic 25.0%
! CATAAGATTCATAGATAGAA+AGG - chr4.1:13275607-13275626 None:intergenic 25.0%
! GACTTAAAGTCATAACTATA+AGG - chr4.1:13277093-13277112 None:intergenic 25.0%
! TAAAAAAATTCAAACAGGTC+GGG - chr4.1:13276625-13276644 None:intergenic 25.0%
! TAAATAACCAATAACAGCAA+CGG - chr4.1:13276995-13277014 None:intergenic 25.0%
! TATATGATGATATGATCTCT+TGG + chr4.1:13276085-13276104 MS.gene53261:intron 25.0%
! TATGCATAAAAATCAACACT+CGG - chr4.1:13277170-13277189 None:intergenic 25.0%
! TATGCATTTGAAGGAATTAA+AGG - chr4.1:13277570-13277589 None:intergenic 25.0%
! TTAAAGTCATAACTATAAGG+TGG - chr4.1:13277090-13277109 None:intergenic 25.0%
! TTAGATGATTATGCATTTGA+AGG - chr4.1:13277579-13277598 None:intergenic 25.0%
! TTAGTGAAAGTGATGAATTA+GGG - chr4.1:13275281-13275300 None:intergenic 25.0%
! TTTACTAGTAATGACAAAGT+TGG + chr4.1:13278072-13278091 MS.gene53261:intron 25.0%
!! TTTTACTCATACTGTAATGT+TGG - chr4.1:13276211-13276230 None:intergenic 25.0%
!!! ACATTTCTAGATGATTGTTT+TGG + chr4.1:13275944-13275963 MS.gene53261:intron 25.0%
!!! GAAATGTTGATAATTTGACT+GGG + chr4.1:13278483-13278502 MS.gene53261:CDS 25.0%
!!! GAATTTTTTTAAAGCCTTTG+TGG + chr4.1:13276634-13276653 MS.gene53261:intron 25.0%
!!! GGCTTTAAAAAAATTCAAAC+AGG - chr4.1:13276630-13276649 None:intergenic 25.0%
!!! GTTGTATTTGCTATGATATA+TGG + chr4.1:13275919-13275938 MS.gene53261:intron 25.0%
!!! TGTGTTTTTGTTTTGAATGT+TGG + chr4.1:13277425-13277444 MS.gene53261:intron 25.0%
!!! TGTTTTTATTGTTTGGACTT+TGG + chr4.1:13276056-13276075 MS.gene53261:intron 25.0%
!!! TTTTTTGTTAGTTTCATGGT+TGG + chr4.1:13277489-13277508 MS.gene53261:intron 25.0%
AATCAGAATATCTCAAGAGT+AGG - chr4.1:13279010-13279029 None:intergenic 30.0%
AATGACTTTACAGTGGAAAA+AGG - chr4.1:13277745-13277764 None:intergenic 30.0%
ACAATTCATGTTATGAGTTC+AGG + chr4.1:13277221-13277240 MS.gene53261:intron 30.0%
ATAAAACTCATGCATTACCA+AGG - chr4.1:13277263-13277282 None:intergenic 30.0%
ATGACTTTACAGTGGAAAAA+GGG - chr4.1:13277744-13277763 None:intergenic 30.0%
ATGAGACGGAAACATAAAAA+TGG - chr4.1:13275870-13275889 None:intergenic 30.0%
ATGCATAGCTAAAAAAAAGG+AGG - chr4.1:13278190-13278209 None:intergenic 30.0%
ATGTCAATTTCAGATCTTCT+GGG + chr4.1:13278397-13278416 MS.gene53261:intron 30.0%
ATTGCAAACATAGAGAAGTA+GGG + chr4.1:13276147-13276166 MS.gene53261:intron 30.0%
CATAATCATCTAAAAGCCTA+TGG + chr4.1:13277586-13277605 MS.gene53261:intron 30.0%
GAAATAGAAATCATATCACC+AGG - chr4.1:13278127-13278146 None:intergenic 30.0%
GACTTGTGAAATTGAGTTAT+TGG + chr4.1:13277045-13277064 MS.gene53261:intron 30.0%
GGCAAAAAGCAATAAATGTT+GGG - chr4.1:13276394-13276413 None:intergenic 30.0%
GGCAATATGTATTCTTTCTT+GGG + chr4.1:13278504-13278523 MS.gene53261:CDS 30.0%
GTCCAAACAATAAAAACATC+AGG - chr4.1:13276054-13276073 None:intergenic 30.0%
GTGATGCATAGCTAAAAAAA+AGG - chr4.1:13278193-13278212 None:intergenic 30.0%
GTTAGTGAAAGTGATGAATT+AGG - chr4.1:13275282-13275301 None:intergenic 30.0%
GTTTACACAGAATCATGATT+GGG + chr4.1:13276519-13276538 MS.gene53261:intron 30.0%
TACTCTTGAGATATTCTGAT+TGG + chr4.1:13279009-13279028 MS.gene53261:intron 30.0%
TATGTCAATTTCAGATCTTC+TGG + chr4.1:13278396-13278415 MS.gene53261:intron 30.0%
TGTTTACACAGAATCATGAT+TGG + chr4.1:13276518-13276537 MS.gene53261:intron 30.0%
! AACAATTGACAGGTTTTCTA+TGG - chr4.1:13278221-13278240 None:intergenic 30.0%
! AGTGTTTAAGCCAAACATTT+TGG + chr4.1:13277968-13277987 MS.gene53261:intron 30.0%
! ATAGCAAAGCATTCCAAAAT+AGG - chr4.1:13277298-13277317 None:intergenic 30.0%
! GTGGATTATACATTCTCAAT+TGG + chr4.1:13278894-13278913 MS.gene53261:CDS 30.0%
! GTTTTACGATTTTACTACCT+TGG + chr4.1:13277243-13277262 MS.gene53261:intron 30.0%
! TGTTGTTTATTTGGCTGAAT+TGG + chr4.1:13278694-13278713 MS.gene53261:intron 30.0%
! TTTGTTCAGCTTTCCTATTT+TGG + chr4.1:13277282-13277301 MS.gene53261:intron 30.0%
!! AAAGGATTAGCTGAAAAAAC+AGG + chr4.1:13276247-13276266 MS.gene53261:intron 30.0%
!! GATAATGACGAAAAGTATCA+AGG - chr4.1:13275519-13275538 None:intergenic 30.0%
!! GGAAATGTTGATAATTTGAC+TGG + chr4.1:13278482-13278501 MS.gene53261:CDS 30.0%
!! GTCCTGATGTTTTTATTGTT+TGG + chr4.1:13276049-13276068 MS.gene53261:intron 30.0%
!!! AGATTCAGTTTTGAGATGAA+GGG - chr4.1:13276748-13276767 None:intergenic 30.0%
!!! AGGATTTTGGTTGTAAAAAC+AGG - chr4.1:13276906-13276925 None:intergenic 30.0%
!!! GGATTTTGGTTGTAAAAACA+GGG - chr4.1:13276905-13276924 None:intergenic 30.0%
AAAACAAGCAACAACAGAAG+AGG - chr4.1:13276926-13276945 None:intergenic 35.0%
AAATACAACACATCCTCGAA+TGG - chr4.1:13275908-13275927 None:intergenic 35.0%
AAATTCATGCCATCTTCTTC+TGG + chr4.1:13278036-13278055 MS.gene53261:intron 35.0%
AAGCAGTCAAAACATAAAGG+TGG - chr4.1:13278568-13278587 None:intergenic 35.0%
AATAGAATTCAGTACGGACA+GGG - chr4.1:13278275-13278294 None:intergenic 35.0%
ACAAAAATAGAGCACAAGAG+AGG - chr4.1:13276802-13276821 None:intergenic 35.0%
ACATTTCTTAGAGGACAATC+AGG - chr4.1:13278623-13278642 None:intergenic 35.0%
ACGTTATTTGGATCTGTTCA+CGG + chr4.1:13275381-13275400 MS.gene53261:CDS 35.0%
ATTGTGACCTGAACATGATT+TGG - chr4.1:13275237-13275256 None:intergenic 35.0%
CAAAAATAGAGCACAAGAGA+GGG - chr4.1:13276801-13276820 None:intergenic 35.0%
CATTGCAAACATAGAGAAGT+AGG + chr4.1:13276146-13276165 MS.gene53261:intron 35.0%
CTTAAACACTGCATGATACT+TGG - chr4.1:13277958-13277977 None:intergenic 35.0%
GCACGTAATAGAATTCAGTA+CGG - chr4.1:13278281-13278300 None:intergenic 35.0%
GGGCAAAAAGCAATAAATGT+TGG - chr4.1:13276395-13276414 None:intergenic 35.0%
GGGCAATATGTATTCTTTCT+TGG + chr4.1:13278503-13278522 MS.gene53261:CDS 35.0%
GTGGGATAAAATGAATTTCC+TGG + chr4.1:13278106-13278125 MS.gene53261:intron 35.0%
TAATAGAATTCAGTACGGAC+AGG - chr4.1:13278276-13278295 None:intergenic 35.0%
TACTGAAATCGGAAAGAACA+TGG - chr4.1:13276590-13276609 None:intergenic 35.0%
TAGGGATTGTGAAATGTGAA+GGG - chr4.1:13275263-13275282 None:intergenic 35.0%
TCATAGCTTCTCTTAACCAT+AGG - chr4.1:13275490-13275509 None:intergenic 35.0%
TCTTTCCGATTTCAGTAACT+GGG + chr4.1:13276593-13276612 MS.gene53261:intron 35.0%
TGTGTTCTTAGCACGTTATT+TGG + chr4.1:13275369-13275388 MS.gene53261:CDS 35.0%
TTAGGGATTGTGAAATGTGA+AGG - chr4.1:13275264-13275283 None:intergenic 35.0%
TTCTATTTCCTTCTTCCGTA+TGG + chr4.1:13278138-13278157 MS.gene53261:intron 35.0%
TTCTTTCCGATTTCAGTAAC+TGG + chr4.1:13276592-13276611 MS.gene53261:intron 35.0%
TTGCAAACATAGAGAAGTAG+GGG + chr4.1:13276148-13276167 MS.gene53261:intron 35.0%
! CTGGGCATTTTCAATATACT+TGG + chr4.1:13278415-13278434 MS.gene53261:CDS 35.0%
! GGCATTTTCAATATACTTGG+AGG + chr4.1:13278418-13278437 MS.gene53261:CDS 35.0%
!!! AAGGGAATTGGTTTTTAGCA+AGG - chr4.1:13276730-13276749 None:intergenic 35.0%
!!! AAGTGTTGGTGCTACATTTT+CGG + chr4.1:13275802-13275821 MS.gene53261:intron 35.0%
!!! AGGGAATTGGTTTTTAGCAA+GGG - chr4.1:13276729-13276748 None:intergenic 35.0%
!!! AGTTTTGAGATGAAGGGAAT+TGG - chr4.1:13276742-13276761 None:intergenic 35.0%
!!! ATTTGGTAGCGTAGATTTTG+AGG - chr4.1:13275220-13275239 None:intergenic 35.0%
!!! CAGATTCAGTTTTGAGATGA+AGG - chr4.1:13276749-13276768 None:intergenic 35.0%
!!! CTGTTCTTTTCTAGAGTTCT+TGG - chr4.1:13277800-13277819 None:intergenic 35.0%
!!! TTCTAGATGATTGTTTTGGC+TGG + chr4.1:13275948-13275967 MS.gene53261:intron 35.0%
ACGGACAGGGAATATATTCT+TGG - chr4.1:13278262-13278281 None:intergenic 40.0%
AGGGATTGTGAAATGTGAAG+GGG - chr4.1:13275262-13275281 None:intergenic 40.0%
AGTCTTCATTGCTAGTTCGT+TGG + chr4.1:13275438-13275457 MS.gene53261:CDS 40.0%
ATAGATAGAAAGGGACCTCA+AGG - chr4.1:13275597-13275616 None:intergenic 40.0%
ATTTGAGTGACGCTTAACCT+AGG + chr4.1:13276005-13276024 MS.gene53261:intron 40.0%
CAGGGAATATATTCTTGGAC+AGG - chr4.1:13278257-13278276 None:intergenic 40.0%
CTTCTCTATGTTTGCAATGC+AGG - chr4.1:13276145-13276164 None:intergenic 40.0%
GTAAAAACAGGGTACAAGAG+AGG - chr4.1:13276894-13276913 None:intergenic 40.0%
TACGCTACCAAATCATGTTC+AGG + chr4.1:13275227-13275246 MS.gene53261:CDS 40.0%
TCGTACAAGTCTACGATTTC+TGG + chr4.1:13276478-13276497 MS.gene53261:intron 40.0%
TCTGTCCCAGTTACTGAAAT+CGG - chr4.1:13276601-13276620 None:intergenic 40.0%
TTAAATTGCAGTGCGTATCG+TGG + chr4.1:13278875-13278894 MS.gene53261:intron 40.0%
TTTCATGGTTGGAGAGGAAT+GGG + chr4.1:13277500-13277519 MS.gene53261:intron 40.0%
TTTCGCAGATGCAAATGAGA+CGG - chr4.1:13275884-13275903 None:intergenic 40.0%
TTTGAGTGACGCTTAACCTA+GGG + chr4.1:13276006-13276025 MS.gene53261:intron 40.0%
! ATTTTATCCCACACTGTGAG+CGG - chr4.1:13278098-13278117 None:intergenic 40.0%
! CTAGTTCGTTGGCTATTGTT+TGG + chr4.1:13275449-13275468 MS.gene53261:CDS 40.0%
! GCAACAACAGAAGAGGATTT+TGG - chr4.1:13276919-13276938 None:intergenic 40.0%
! TCTAACTCCGTTGCTGTTAT+TGG + chr4.1:13276985-13277004 MS.gene53261:intron 40.0%
! TGTTAGTTTCATGGTTGGAG+AGG + chr4.1:13277494-13277513 MS.gene53261:intron 40.0%
! TGTTGGAGCATCTCCAAAAT+TGG + chr4.1:13277442-13277461 MS.gene53261:intron 40.0%
!! TCTTGGGCATTTTGTTTCGA+GGG + chr4.1:13276102-13276121 MS.gene53261:intron 40.0%
!!! AGTGTTGGTGCTACATTTTC+GGG + chr4.1:13275803-13275822 MS.gene53261:intron 40.0%
AACGTGGCTCAGTCAAATAG+CGG - chr4.1:13278925-13278944 None:intergenic 45.0%
AATCCATAACCCTGTTCCCT+AGG - chr4.1:13276025-13276044 None:intergenic 45.0%
AGCAGATTCCATACGGAAGA+AGG - chr4.1:13278149-13278168 None:intergenic 45.0%
ATAACCCTAGCCACCATTGT+TGG - chr4.1:13276177-13276196 None:intergenic 45.0%
ATAGAAAGGGACCTCAAGGA+AGG - chr4.1:13275593-13275612 None:intergenic 45.0%
ATGAACATCTTCAGACTCGC+CGG + chr4.1:13275158-13275177 MS.gene53261:CDS 45.0%
CGAGAAATTCACCTTCCTTG+AGG + chr4.1:13275579-13275598 MS.gene53261:CDS 45.0%
GAAGTAGGGGTAACCAACAA+TGG + chr4.1:13276161-13276180 MS.gene53261:intron 45.0%
GAATTGCAGCCAGAAGAAGA+TGG - chr4.1:13278048-13278067 None:intergenic 45.0%
GGAGACTGAACACTGTGATT+CGG + chr4.1:13277525-13277544 MS.gene53261:intron 45.0%
GTAACCAACAATGGTGGCTA+GGG + chr4.1:13276170-13276189 MS.gene53261:intron 45.0%
GTACAAGAGAGGATGTGTTC+TGG - chr4.1:13276883-13276902 None:intergenic 45.0%
GTTTCATGGTTGGAGAGGAA+TGG + chr4.1:13277499-13277518 MS.gene53261:intron 45.0%
TAACCTAGGGAACAGGGTTA+TGG + chr4.1:13276019-13276038 MS.gene53261:intron 45.0%
TAACGAATGCTGCGTTGAAG+TGG + chr4.1:13278347-13278366 MS.gene53261:intron 45.0%
TCGTTGCAACAAAACCAGCT+GGG - chr4.1:13278459-13278478 None:intergenic 45.0%
TGACACTGACAGTTTGACAC+TGG + chr4.1:13275705-13275724 MS.gene53261:intron 45.0%
TGTAGCACCAACACTTCTGA+TGG - chr4.1:13275798-13275817 None:intergenic 45.0%
TTCGTTGCAACAAAACCAGC+TGG - chr4.1:13278460-13278479 None:intergenic 45.0%
! CTTCTTCTGGCTGCAATTCA+TGG + chr4.1:13278049-13278068 MS.gene53261:intron 45.0%
!! ACTGGTCTTATTGCCAAACC+AGG - chr4.1:13276671-13276690 None:intergenic 45.0%
!! CTCTTGGGCATTTTGTTTCG+AGG + chr4.1:13276101-13276120 MS.gene53261:intron 45.0%
!! TCTACGATTTCTGGAGTGCT+TGG + chr4.1:13276487-13276506 MS.gene53261:intron 45.0%
!! TGGTAGCGTAGATTTTGAGG+AGG - chr4.1:13275217-13275236 None:intergenic 45.0%
!! TGTTGCTGCTAGCTTTCTTC+TGG + chr4.1:13275543-13275562 MS.gene53261:CDS 45.0%
!!! CTGGTTTTGTTGCAACGAAG+TGG + chr4.1:13278461-13278480 MS.gene53261:CDS 45.0%
AAGATCGCGTGAAATCGCTG+TGG + chr4.1:13276543-13276562 MS.gene53261:intron 50.0%
AGGTCAGAGTTCAGCCACAA+AGG - chr4.1:13276651-13276670 None:intergenic 50.0%
ATGGTTGGAGAGGAATGGGT+TGG + chr4.1:13277504-13277523 MS.gene53261:intron 50.0%
CAGCTGCAGCAGATTCCATA+CGG - chr4.1:13278156-13278175 None:intergenic 50.0%
CCACGTTTCACTCGATGGAT+AGG + chr4.1:13278938-13278957 MS.gene53261:CDS 50.0%
CCTATCCATCGAGTGAAACG+TGG - chr4.1:13278941-13278960 None:intergenic 50.0%
CGCGTAGAAGCAGAAGATGT+TGG - chr4.1:13276848-13276867 None:intergenic 50.0%
CGTTGCAACAAAACCAGCTG+GGG - chr4.1:13278458-13278477 None:intergenic 50.0%
CTGAACAACACCTGAGACAC+AGG - chr4.1:13279051-13279070 None:intergenic 50.0%
GACGCTTAACCTAGGGAACA+GGG + chr4.1:13276013-13276032 MS.gene53261:intron 50.0%
GAGGAGATGAGTCATGTCAC+CGG - chr4.1:13275180-13275199 None:intergenic 50.0%
GGTAACCAACAATGGTGGCT+AGG + chr4.1:13276169-13276188 MS.gene53261:intron 50.0%
GTAGGGGTAACCAACAATGG+TGG + chr4.1:13276164-13276183 MS.gene53261:intron 50.0%
GTGCATGCGTGTTCATCCTA+TGG + chr4.1:13275471-13275490 MS.gene53261:CDS 50.0%
TAGCAAGGGAAACGACGCAA+CGG - chr4.1:13276715-13276734 None:intergenic 50.0%
TGACGCTTAACCTAGGGAAC+AGG + chr4.1:13276012-13276031 MS.gene53261:intron 50.0%
TTTGTGGCTGAACTCTGACC+TGG + chr4.1:13276650-13276669 MS.gene53261:intron 50.0%
! CACGCATCCATCAGAAGTGT+TGG + chr4.1:13275788-13275807 MS.gene53261:intron 50.0%
! CACTTCTGATGGATGCGTGT+TGG - chr4.1:13275787-13275806 None:intergenic 50.0%
! GTTTTTGCAGCCTGTGTCTC+AGG + chr4.1:13279038-13279057 MS.gene53261:intron 50.0%
AAAGTTGGCCGCTCACAGTG+TGG + chr4.1:13278087-13278106 MS.gene53261:intron 55.0%
AAGTTGGCCGCTCACAGTGT+GGG + chr4.1:13278088-13278107 MS.gene53261:intron 55.0%
AGCAAGGGAAACGACGCAAC+GGG - chr4.1:13276714-13276733 None:intergenic 55.0%
AGTTGCAATACTGCCCCAGC+TGG + chr4.1:13278442-13278461 MS.gene53261:CDS 55.0%
CTGAGCCACGTTTCACTCGA+TGG + chr4.1:13278933-13278952 MS.gene53261:CDS 55.0%
GCATCTGCGAAAGCCATTCG+AGG + chr4.1:13275892-13275911 MS.gene53261:intron 55.0%
GGAGGAGAACGAGAATGCTG+AGG - chr4.1:13275199-13275218 None:intergenic 55.0%
GGCTGAACTCTGACCTGGTT+TGG + chr4.1:13276655-13276674 MS.gene53261:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13275158 13279111 13275158 ID=MS.gene53261
chr4.1 mRNA 13275158 13279111 13275158 ID=MS.gene53261.t1;Parent=MS.gene53261
chr4.1 exon 13275158 13275248 13275158 ID=MS.gene53261.t1.exon1;Parent=MS.gene53261.t1
chr4.1 CDS 13275158 13275248 13275158 ID=cds.MS.gene53261.t1;Parent=MS.gene53261.t1
chr4.1 exon 13275335 13275600 13275335 ID=MS.gene53261.t1.exon2;Parent=MS.gene53261.t1
chr4.1 CDS 13275335 13275600 13275335 ID=cds.MS.gene53261.t1;Parent=MS.gene53261.t1
chr4.1 exon 13278410 13278525 13278410 ID=MS.gene53261.t1.exon3;Parent=MS.gene53261.t1
chr4.1 CDS 13278410 13278525 13278410 ID=cds.MS.gene53261.t1;Parent=MS.gene53261.t1
chr4.1 exon 13278886 13278959 13278886 ID=MS.gene53261.t1.exon4;Parent=MS.gene53261.t1
chr4.1 CDS 13278886 13278959 13278886 ID=cds.MS.gene53261.t1;Parent=MS.gene53261.t1
chr4.1 exon 13279048 13279111 13279048 ID=MS.gene53261.t1.exon5;Parent=MS.gene53261.t1
chr4.1 CDS 13279048 13279111 13279048 ID=cds.MS.gene53261.t1;Parent=MS.gene53261.t1
Gene Sequence

>MS.gene53261

ATGAACATCTTCAGACTCGCCGGTGACATGACTCATCTCCTCAGCATTCTCGTTCTCCTCCTCAAAATCTACGCTACCAAATCATGTTCAGGTCACAATTTCCCCTTCACATTTCACAATCCCTAATTCATCACTTTCACTAACAATTGATCATGATTTTTTTCTCTTCAATTTCAGGTGTTTCTCGTAAGACTCAAGAACTATACGCGATTGTGTTCTTAGCACGTTATTTGGATCTGTTCACGGATTTCATTTCCGTTTATAATACGTTTATGAAGATAGTCTTCATTGCTAGTTCGTTGGCTATTGTTTGGTGCATGCGTGTTCATCCTATGGTTAAGAGAAGCTATGATAAAGACCTTGATACTTTTCGTCATTATCTTCTTGTTGCTGCTAGCTTTCTTCTGGCACTCGTCTTGCACGAGAAATTCACCTTCCTTGAGGTCCCTTTCTATCTATGAATCTTATGTTTCTCTTTTTTAAGTATTTTGCAATGTTAATGTTTGAGTTGCGTGATTTAATTGTGTTTTATTTGTTTATTTTACACTGACACTGACAGTTTGACACTGGTAAAAATTTGATTAACTGAATTTTCTCACATGTCAGTGTCTATGATGTATCTGACACCAACACGCATCCATCAGAAGTGTTGGTGCTACATTTTCGGGTTTTTAATCGTTGAGCATTTCTCCAATTGTTTACTTTCATACCATTTTTATGTTTCCGTCTCATTTGCATCTGCGAAAGCCATTCGAGGATGTGTTGTATTTGCTATGATATATGGATACATTTCTAGATGATTGTTTTGGCTGGTCATGATGACAAACTTTAGATAAGCAAGAAACATATTTGAGTGACGCTTAACCTAGGGAACAGGGTTATGGATTTTTTGTCCTGATGTTTTTATTGTTTGGACTTTGGAGTTCTTATATGATGATATGATCTCTTGGGCATTTTGTTTCGAGGGATGATTCTTTTGTTAAACCTGCATTGCAAACATAGAGAAGTAGGGGTAACCAACAATGGTGGCTAGGGTTATATTTGCATCAACCAACATTACAGTATGAGTAAAATAAGGCTATAAAATAAAAAGGATTAGCTGAAAAAACAGGAAAAAACAATCAGTTTATTTCATTTTCATTTTTTGTCTATGTTTCATCGATAATTATTAAAAGAAACATGGTATTTTTTTTAATTATCTATAAAAACAAATCAACTGAAAATCAAACGCAACCCAACATTTATTGCTTTTTGCCCTTTTTCTAACTATTTAATGCACATAGTTATTGAAATCTCGATTTAAAACGAAAAATCGAAAAATCGTACAAGTCTACGATTTCTGGAGTGCTTGGTGATTTCATGTTTACACAGAATCATGATTGGGTAAGATCGCGTGAAATCGCTGTGGAAAAACATAAAGTTGTGCAATCCATGTTCTTTCCGATTTCAGTAACTGGGACAGATCCCGACCTGTTTGAATTTTTTTAAAGCCTTTGTGGCTGAACTCTGACCTGGTTTGGCAATAAGACCAGTTTCTGTTTTATTTATTCATACCCGTTGCGTCGTTTCCCTTGCTAAAAACCAATTCCCTTCATCTCAAAACTGAATCTGTAATGCTTTGTTTTTGTGAGCATAACTCACCCTCTCTTGTGCTCTATTTTTGTGTTCATGTGCTTTGTTTTTGCGACCAACATCTTCTGCTTCTACGCGCTGTTTTTGCAACCAGAACACATCCTCTCTTGTACCCTGTTTTTACAACCAAAATCCTCTTCTGTTGTTGCTTGTTTTTGCTGCTAAGTTCTGTTCTTAATTCGATTCATTAGAATGTCTAACTCCGTTGCTGTTATTGGTTATTTAAGCAAGCCTAAATTAAGTTGATTTGTTCATGACTTGTGAAATTGAGTTATTGGTTATTTGAGAACTTATTTACCACCTTATAGTTATGACTTTAAGTCTAATTAAGTATTTGAATCTGAAGACTTACTTTCACTTACTAATGTTAATTTATGCCGAGTGTTGATTTTTATGCATATTATATGAGTTTAATGAATTTGTAGAATCTTACAATTCATGTTATGAGTTCAGGTTTTACGATTTTACTACCTTGGTAATGCATGAGTTTTATTTGTTCAGCTTTCCTATTTTGGAATGCTTTGCTATGCAATTACATTTTTCTCTTTGAGATCAAATGCAAATATAAGTAAATCTTGACAAATTTCATCCCTTTTGAAATTGCGATAAAAAATGCGATGTTTCGAAAAATAATCTGTGTTTTTGTTTTGAATGTTGGAGCATCTCCAAAATTGGTTTATAGAACTAAAAACCCTGTTTTTTTTTGTTAGTTTCATGGTTGGAGAGGAATGGGTTGGAGACTGAACACTGTGATTCGGTTTAAGATCTGATTTTTGTCCTTTAATTCCTTCAAATGCATAATCATCTAAAAGCCTATGGATAAGTTTCATTTCATATTTTCATGCAAATTTTGCATATTTGAGCAAAGTTGATAGACATTAATATTCTATCAAATGTGTTGAATCTGACGATATTTCAAAACTTATCTGATCACTTGTGACATTCTTTAATCCCTTTTTCCACTGTAAAGTCATTTCTTTATATGTTTGAACGTTGCTCATAGAAAACCAAGAACTCTAGAAAAGAACAGCTTCAGATATGTTGAGTGTTTCTTAATGTCAAGTGTCTCTGAACATCTTGATTATTTGTGTCAAATCTTGTAACATGTTAACAAGAACAAGTTTTTAATCAAATGGTTTTATTTTAGTATAATTTGAAAGGATGGCCAAGTATCATGCAGTGTTTAAGCCAAACATTTTGGACAAAGATATATTTTGTTGATAGTTTGAAATATTTAACATTTATAAAATTCATGCCATCTTCTTCTGGCTGCAATTCATGGTTTACTAGTAATGACAAAGTTGGCCGCTCACAGTGTGGGATAAAATGAATTTCCTGGTGATATGATTTCTATTTCCTTCTTCCGTATGGAATCTGCTGCAGCTGTGATTTATTTTCCTCCTTTTTTTTAGCTATGCATCACAAAAGCCATAGAAAACCTGTCAATTGTTTTCAAATCTTATTCCTGTCCAAGAATATATTCCCTGTCCGTACTGAATTCTATTACGTGCGCTACATGTTAGCTTTATAGTAATTCTGTTACACAATGTAACATAATAACGAATGCTGCGTTGAAGTGGAAATGCTCAATATACTTTGTTTTATGTATGTCAATTTCAGATCTTCTGGGCATTTTCAATATACTTGGAGGCAGTTGCAATACTGCCCCAGCTGGTTTTGTTGCAACGAAGTGGAAATGTTGATAATTTGACTGGGCAATATGTATTCTTTCTTGGGTATGTGCTCTTTTGTTTATTTAGCTTCTCTTTTACGATCCACCTTTATGTTTTGACTGCTTTATACTATGAACTTTCTAGATACTTTTGTCATCCTGATTGTCCTCTAAGAAATGTATTTTCTCTACTTGATAATTGATATATGTAATGGTTGTCATTATTTGAAAATGTTGTTTATTTGGCTGAATTGGCATTTTTCTGCTGACGTGTTGTCTAACAACTTGTCTCTAGTCTTCATGACTGCTTAGTTTCATGAGCAGTTTGCTGTAGAACTTTTAAATATTACTAGACAATCTGATCTTGTTCAAAATCTGTTTATTATGATAAGTAAATATCATACCATTTATTTTTAAATTGCAGTGCGTATCGTGGATTATACATTCTCAATTGGATATACCGCTATTTGACTGAGCCACGTTTCACTCGATGGATAGGTAAATATGCTTAAAATCAAAATATGAATTGCTATTTAAACTATATGCCTACTCTTGAGATATTCTGATTGGCTTCTTGTTTTTGCAGCCTGTGTCTCAGGTGTTGTTCAGACAGCTCTGTATGCTGATTTCTTCTACTATTACTTCATCAG
Protein sequence

>MS.gene53261.t1

MNIFRLAGDMTHLLSILVLLLKIYATKSCSGVSRKTQELYAIVFLARYLDLFTDFISVYNTFMKIVFIASSLAIVWCMRVHPMVKRSYDKDLDTFRHYLLVAASFLLALVLHEKFTFLEIFWAFSIYLEAVAILPQLVLLQRSGNVDNLTGQYVFFLGAYRGLYILNWIYRYLTEPRFTRWIACVSGVVQTALYADFFYYYFI