AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53287


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53287.t1 MTR_1g073990 96.552 116 4 0 1 116 93 208 1.93e-75 227
MS.gene53287.t1 MTR_1g074070 83.621 116 18 1 1 116 93 207 4.81e-65 201
MS.gene53287.t1 MTR_1g074020 77.586 116 25 1 1 116 90 204 2.74e-60 188
MS.gene53287.t1 MTR_1g074120 80.172 116 22 1 1 116 93 207 5.76e-60 187
MS.gene53287.t1 MTR_1g074160 76.724 116 26 1 1 116 90 204 2.73e-59 183
MS.gene53287.t1 MTR_1g074160 76.724 116 26 1 1 116 90 204 3.23e-58 183
MS.gene53287.t1 MTR_1g051120 66.667 117 38 1 1 116 93 209 2.58e-49 156
MS.gene53287.t1 MTR_1g051120 66.667 117 38 1 1 116 93 209 1.85e-48 155
MS.gene53287.t1 MTR_1g051120 66.667 117 38 1 1 116 93 209 2.15e-47 155
MS.gene53287.t1 MTR_1g070175 47.414 116 58 1 1 116 93 205 9.91e-32 114
MS.gene53287.t1 MTR_3g012420 39.130 115 68 1 4 116 97 211 2.51e-20 84.7
MS.gene53287.t1 MTR_7g100050 42.373 118 52 5 4 111 93 204 7.84e-20 83.2
MS.gene53287.t1 MTR_1g030580 36.885 122 60 2 4 116 96 209 3.49e-19 81.3
MS.gene53287.t1 MTR_8g041390 47.500 80 38 1 4 79 93 172 3.55e-19 81.3
MS.gene53287.t1 MTR_6g036450 40.000 120 62 4 4 116 95 211 8.50e-18 77.4
MS.gene53287.t1 MTR_3g072530 33.333 111 73 1 7 116 97 207 9.05e-18 77.0
MS.gene53287.t1 MTR_3g072530 33.333 111 73 1 7 116 97 207 1.56e-17 76.6
MS.gene53287.t1 MTR_1g068630 37.815 119 61 2 4 116 95 206 2.77e-17 75.9
MS.gene53287.t1 MTR_3g072500 32.500 120 70 2 4 116 100 215 7.09e-17 73.2
MS.gene53287.t1 MTR_1g014790 39.655 116 61 4 7 116 46 158 9.08e-17 72.8
MS.gene53287.t1 MTR_3g072500 32.500 120 70 2 4 116 100 215 1.72e-16 73.2
MS.gene53287.t1 MTR_1g014510 40.517 116 60 4 7 116 95 207 1.98e-16 73.9
MS.gene53287.t1 MTR_3g072500 32.500 120 70 2 4 116 100 215 2.93e-16 73.2
MS.gene53287.t1 MTR_6g007740 37.838 111 63 3 7 115 95 201 4.01e-16 73.2
MS.gene53287.t1 MTR_1g030600 35.965 114 68 3 7 116 101 213 4.17e-16 72.8
MS.gene53287.t1 MTR_4g094342 33.065 124 69 4 3 116 92 211 7.82e-16 71.2
MS.gene53287.t1 MTR_3g090550 39.394 99 60 0 7 105 88 186 8.45e-16 71.6
MS.gene53287.t1 MTR_4g094340 33.051 118 73 2 4 116 106 222 8.64e-16 70.5
MS.gene53287.t1 MTR_5g021160 36.667 120 71 2 1 116 1 119 9.86e-16 71.2
MS.gene53287.t1 MTR_4g094340 33.051 118 73 2 4 116 88 204 1.20e-15 70.9
MS.gene53287.t1 MTR_4g094340 33.051 118 73 2 4 116 22 138 1.25e-15 70.9
MS.gene53287.t1 MTR_4g094342 33.065 124 69 4 3 116 92 211 1.61e-15 71.2
MS.gene53287.t1 MTR_6g081840 33.600 125 64 4 4 116 90 207 1.62e-15 71.2
MS.gene53287.t1 MTR_4g094340 33.051 118 73 2 4 116 106 222 1.62e-15 70.5
MS.gene53287.t1 MTR_3g072560 32.727 110 73 1 7 116 101 209 1.65e-15 71.2
MS.gene53287.t1 MTR_5g021160 36.667 120 71 2 1 116 84 202 1.93e-15 70.1
MS.gene53287.t1 MTR_4g094340 33.051 118 73 2 4 116 88 204 2.18e-15 70.9
MS.gene53287.t1 MTR_5g021160 36.667 120 71 2 1 116 84 202 2.42e-15 70.5
MS.gene53287.t1 MTR_4g094340 33.051 118 73 2 4 116 106 222 2.49e-15 70.5
MS.gene53287.t1 MTR_4g094362 37.607 117 68 3 4 116 94 209 2.73e-15 70.5
MS.gene53287.t1 MTR_2g007960 34.426 122 71 3 4 116 92 213 8.34e-15 69.3
MS.gene53287.t1 MTR_3g072500 31.304 115 68 2 9 116 2 112 9.24e-15 68.6
MS.gene53287.t1 MTR_2g007990 33.607 122 73 2 1 116 92 211 1.63e-14 68.6
MS.gene53287.t1 MTR_1g038560 33.333 114 74 2 5 116 82 195 2.15e-14 67.8
MS.gene53287.t1 MTR_2g007970 33.333 117 73 3 4 116 92 207 3.67e-14 67.4
MS.gene53287.t1 MTR_3g112480 36.220 127 67 2 4 116 45 171 3.75e-14 67.0
MS.gene53287.t1 MTR_3g072520 33.333 111 71 1 9 116 94 204 3.96e-14 67.4
MS.gene53287.t1 MTR_4g094352 35.714 126 65 4 3 116 92 213 4.52e-14 67.0
MS.gene53287.t1 MTR_3g112480 36.220 127 67 2 4 116 100 226 5.22e-14 67.0
MS.gene53287.t1 MTR_0096s0100 30.909 110 76 0 7 116 101 210 8.67e-14 66.2
MS.gene53287.t1 MTR_4g129650 36.752 117 65 3 4 116 91 202 9.23e-14 66.2
MS.gene53287.t1 MTR_6g007753 35.398 113 67 3 5 115 377 485 1.60e-13 65.9
MS.gene53287.t1 MTR_2g027030 35.043 117 71 4 4 116 92 207 4.34e-13 64.3
MS.gene53287.t1 MTR_6g007743 35.714 112 64 3 7 115 95 201 4.71e-13 64.3
MS.gene53287.t1 MTR_6g007750 34.513 113 69 3 5 115 95 204 6.81e-13 63.9
MS.gene53287.t1 MTR_3g095420 33.884 121 75 2 1 116 86 206 8.40e-13 63.5
MS.gene53287.t1 MTR_4g094348 28.571 126 76 3 3 116 1 124 1.09e-12 61.2
MS.gene53287.t1 MTR_6g007760 38.261 115 66 4 5 116 92 204 1.37e-12 62.8
MS.gene53287.t1 MTR_6g007747 40.845 71 42 0 5 75 91 161 2.59e-12 62.0
MS.gene53287.t1 MTR_2g102340 33.621 116 71 3 4 116 92 204 3.05e-12 62.0
MS.gene53287.t1 MTR_8g006050 32.759 116 75 2 4 116 75 190 3.48e-12 61.6
MS.gene53287.t1 MTR_5g034930 45.161 62 34 0 9 70 91 152 4.38e-12 61.2
MS.gene53287.t1 MTR_8g006010 33.333 114 75 1 4 116 90 203 4.48e-12 61.6
MS.gene53287.t1 MTR_3g095440 32.203 118 75 2 4 116 87 204 5.69e-12 60.5
MS.gene53287.t1 MTR_2g065550 30.973 113 78 0 4 116 92 204 6.09e-12 61.2
MS.gene53287.t1 MTR_5g034990 36.134 119 56 4 7 116 96 203 6.72e-12 60.8
MS.gene53287.t1 MTR_2g102340 33.621 116 71 3 4 116 118 230 6.88e-12 60.8
MS.gene53287.t1 MTR_1g023180 41.429 70 41 0 4 73 101 170 9.28e-12 60.5
MS.gene53287.t1 MTR_3g095440 32.203 118 75 2 4 116 87 204 1.06e-11 60.5
MS.gene53287.t1 MTR_5g034970 30.702 114 70 3 8 116 91 200 1.72e-11 59.7
MS.gene53287.t1 MTR_4g094360 33.333 117 72 2 5 116 95 210 1.86e-11 59.7
MS.gene53287.t1 MTR_4g094360 33.333 117 72 2 5 116 95 210 1.89e-11 59.7
MS.gene53287.t1 MTR_0103s0090 29.091 110 78 0 7 116 3 112 1.89e-11 59.3
MS.gene53287.t1 MTR_4g094360 33.333 117 72 2 5 116 48 163 2.07e-11 59.7
MS.gene53287.t1 MTR_6g007757 32.759 116 68 4 5 116 94 203 2.38e-11 58.9
MS.gene53287.t1 MTR_6g007757 33.333 114 70 3 5 116 94 203 2.45e-11 59.3
MS.gene53287.t1 MTR_0103s0090 29.091 110 78 0 7 116 94 203 2.77e-11 59.3
MS.gene53287.t1 MTR_5g025340 34.000 100 59 2 5 97 91 190 3.32e-11 58.9
MS.gene53287.t1 MTR_5g034910 31.304 115 66 3 9 116 92 200 4.02e-11 58.5
MS.gene53287.t1 MTR_3g095450 32.231 121 77 2 1 116 84 204 4.41e-11 58.5
MS.gene53287.t1 MTR_3g095460 32.500 120 77 1 1 116 82 201 4.58e-11 58.5
MS.gene53287.t1 MTR_1g114210 30.769 117 73 2 5 116 90 203 9.27e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53287.t1 AT4G24980 35.398 113 71 1 4 116 45 155 1.09e-20 84.3
MS.gene53287.t1 AT5G07050 38.525 122 65 2 4 116 97 217 7.61e-20 83.6
MS.gene53287.t1 AT2G37460 35.398 113 72 1 4 116 94 205 3.02e-19 81.6
MS.gene53287.t1 AT4G08300 35.043 117 72 2 4 116 90 206 5.89e-18 78.2
MS.gene53287.t1 AT1G21890 44.286 70 39 0 4 73 90 159 1.52e-16 74.3
MS.gene53287.t1 AT1G21890 44.286 70 39 0 4 73 90 159 2.24e-16 73.6
MS.gene53287.t1 AT1G44800 32.203 118 77 2 1 116 87 203 1.35e-15 71.2
MS.gene53287.t1 AT3G18200 47.761 67 35 0 4 70 114 180 3.96e-15 70.1
MS.gene53287.t1 AT2G39510 37.736 106 60 4 4 105 87 190 6.78e-15 69.3
MS.gene53287.t1 AT4G08290 34.783 115 68 3 7 116 95 207 9.18e-15 68.6
MS.gene53287.t1 AT1G75500 33.065 124 69 2 4 116 99 219 1.17e-14 68.9
MS.gene53287.t1 AT1G75500 33.065 124 69 2 4 116 99 219 1.17e-14 68.9
MS.gene53287.t1 AT3G56620 33.333 114 71 2 4 116 90 199 1.43e-14 68.6
MS.gene53287.t1 AT4G08290 34.783 115 68 3 7 116 95 207 1.47e-14 68.6
MS.gene53287.t1 AT3G53210 31.356 118 73 3 4 116 89 203 2.04e-14 67.4
MS.gene53287.t1 AT3G53210 31.356 118 73 3 4 116 32 146 2.19e-14 67.8
MS.gene53287.t1 AT3G53210 31.356 118 73 3 4 116 89 203 3.37e-14 67.4
MS.gene53287.t1 AT5G13670 30.579 121 72 3 4 116 87 203 7.70e-14 66.6
MS.gene53287.t1 AT2G40900 42.647 68 39 0 4 71 90 157 1.04e-13 66.2
MS.gene53287.t1 AT3G28050 31.818 110 72 1 7 116 94 200 1.33e-13 65.9
MS.gene53287.t1 AT2G37450 32.759 116 77 1 1 116 64 178 5.38e-13 63.9
MS.gene53287.t1 AT5G40210 43.478 69 39 0 5 73 93 161 2.16e-12 62.4
MS.gene53287.t1 AT5G40230 33.884 121 74 3 1 116 97 216 2.54e-12 62.0
MS.gene53287.t1 AT3G30340 30.702 114 77 1 5 116 91 204 3.41e-12 62.0
MS.gene53287.t1 AT3G30340 30.702 114 77 1 5 116 91 204 3.55e-12 61.2
MS.gene53287.t1 AT1G70260 39.189 74 45 0 1 74 91 164 1.43e-11 60.1
MS.gene53287.t1 AT5G40240 33.628 113 69 3 9 116 104 215 1.49e-11 60.1
MS.gene53287.t1 AT5G40240 33.628 113 69 3 9 116 104 215 1.49e-11 60.1
MS.gene53287.t1 AT2G37450 32.710 107 71 1 10 116 1 106 1.59e-11 59.3
MS.gene53287.t1 AT5G40240 33.628 113 69 3 9 116 118 229 1.72e-11 59.7
MS.gene53287.t1 AT3G28080 36.508 63 40 0 9 71 9 71 5.21e-11 57.8
MS.gene53287.t1 AT1G09380 26.496 117 81 1 5 116 88 204 8.34e-11 57.8
MS.gene53287.t1 AT3G28080 36.508 63 40 0 9 71 9 71 9.43e-11 57.4
MS.gene53287.t1 AT3G28080 36.508 63 40 0 9 71 9 71 9.43e-11 57.4
MS.gene53287.t1 AT3G28080 36.508 63 40 0 9 71 9 71 9.43e-11 57.4
MS.gene53287.t1 AT3G28080 36.508 63 40 0 9 71 9 71 9.43e-11 57.4

Find 0 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTCTATTTTTGTTTT+TGG + 8600:6512-6531 MS.gene53287:intron 10.0%
!!! ACAAAAATAGAAATTTTATA+AGG - 8600:6509-6528 MS.gene53287:intergenic 10.0%
!!! AAATTTTAACCTATTGATTT+CGG + 8600:6683-6702 MS.gene53287:intron 15.0%
!!! ATGTTTTTAGTTTTGTAATT+TGG - 8600:6563-6582 MS.gene53287:intergenic 15.0%
!!! AACTTTTTGGTTGAATAATA+TGG - 8600:6892-6911 MS.gene53287:intergenic 20.0%
!!! TTTTAGTTTTGTAATTTGGA+AGG - 8600:6559-6578 MS.gene53287:intergenic 20.0%
AATGTACCATATAGAAAACG+TGG - 8600:6969-6988 MS.gene53286:intergenic 30.0%
ATAAATGCTCCGAAATCAAT+AGG - 8600:6695-6714 MS.gene53287:intergenic 30.0%
GTAGCATAGCAAAAATTCTT+GGG + 8600:6786-6805 MS.gene53287:CDS 30.0%
GTTCGCAATTCTTGATATAA+TGG + 8600:6461-6480 MS.gene53287:intron 30.0%
!! GGTTGAATAATATGGATTAG+AGG - 8600:6884-6903 MS.gene53287:intergenic 30.0%
!! TCGTTGATAGAAGAACTTTT+TGG - 8600:6905-6924 MS.gene53287:intergenic 30.0%
AACATGGAAGCAAGAAATGT+TGG - 8600:6396-6415 MS.gene53286:intergenic 35.0%
AATTTGGAAGGACACAAAAG+TGG - 8600:6547-6566 MS.gene53286:intergenic 35.0%
CACTGCAATAATGAAGGTAA+TGG - 8600:6433-6452 MS.gene53287:intergenic 35.0%
CATTACCTTCATTATTGCAG+TGG + 8600:6431-6450 MS.gene53287:CDS 35.0%
CGTAGCATAGCAAAAATTCT+TGG + 8600:6785-6804 MS.gene53287:CDS 35.0%
CTAGATTCTTCAACGGATAT+TGG + 8600:6619-6638 MS.gene53287:intron 35.0%
TTCACCATGACACTATACAA+GGG + 8600:6833-6852 MS.gene53287:CDS 35.0%
TTTCACCATGACACTATACA+AGG + 8600:6832-6851 MS.gene53287:CDS 35.0%
!! AGAATTTTTGCTATGCTACG+TGG - 8600:6785-6804 MS.gene53287:intergenic 35.0%
!! ATCAACGAGAGTGGATTAAA+GGG + 8600:6917-6936 MS.gene53287:CDS 35.0%
!! TATCAACGAGAGTGGATTAA+AGG + 8600:6916-6935 MS.gene53287:CDS 35.0%
AACACCAGCTAAGGATATCA+AGG - 8600:6813-6832 MS.gene53286:intergenic 40.0%
AGTTCTTCTATCAACGAGAG+TGG + 8600:6908-6927 MS.gene53287:CDS 40.0%
GACCTGTTATGAGAAACTTG+TGG + 8600:6855-6874 MS.gene53287:CDS 40.0%
GTGAGTGCTAGATTCTTCAA+CGG + 8600:6612-6631 MS.gene53287:intron 40.0%
TGCCACAAGTTTCTCATAAC+AGG - 8600:6860-6879 MS.gene53287:intergenic 40.0%
! GGAAGCTATAGTGTTGAACA+TGG - 8600:6412-6431 MS.gene53287:intergenic 40.0%
! GTTGTGCCACGTTTTCTATA+TGG + 8600:6960-6979 MS.gene53287:CDS 40.0%
! TTGACTTGAATGCAGGATTG+AGG + 8600:6742-6761 MS.gene53287:intron 40.0%
!! AAGAGAGTTGACTTGAATGC+AGG + 8600:6735-6754 MS.gene53287:intron 40.0%
AGGTCCCTTGTATAGTGTCA+TGG - 8600:6840-6859 MS.gene53286:intergenic 45.0%
CATGGTGAAAACACCAGCTA+AGG - 8600:6822-6841 MS.gene53287:intergenic 45.0%
TCATTATTGCAGTGGCACTC+AGG + 8600:6439-6458 MS.gene53287:CDS 45.0%
! GAGTGCCACTGCAATAATGA+AGG - 8600:6439-6458 MS.gene53287:intergenic 45.0%
GGGACCTTGATATCCTTAGC+TGG + 8600:6806-6825 MS.gene53287:CDS 50.0%


Chromosome Type Strat End Strand Name
8600 gene 6351 6994 6351 ID=MS.gene53287
8600 mRNA 6351 6994 6351 ID=MS.gene53287.t1;Parent=MS.gene53287
8600 exon 6351 6460 6351 ID=MS.gene53287.t1.exon1;Parent=MS.gene53287.t1
8600 CDS 6351 6460 6351 ID=cds.MS.gene53287.t1;Parent=MS.gene53287.t1
8600 exon 6757 6994 6757 ID=MS.gene53287.t1.exon2;Parent=MS.gene53287.t1
8600 CDS 6757 6994 6757 ID=cds.MS.gene53287.t1;Parent=MS.gene53287.t1
Gene Sequence

>MS.gene53287

ATGGCACTCAATTTGTACTTTGCAAGCTTAAAGTATACTTCTCCAACATTTCTTGCTTCCATGTTCAACACTATAGCTTCCATTACCTTCATTATTGCAGTGGCACTCAGGTTCGCAATTCTTGATATAATGGTTTAAATTAAATATATTTTTAACCTTATAAAATTTCTATTTTTGTTTTTGGTTCATGTATCCACTTTTGTGTCCTTCCAAATTACAAAACTAAAAACATGCAGTTTTGCAATTTCGAAGAATGAAAAAGTGAGTGCTAGATTCTTCAACGGATATTGGTTATCGATGAAGTCAGTGAATATATATACACGCACTGAAAAAAATTTTAACCTATTGATTTCGGAGCATTTATAATTTTTTTTTTTTATCTATAAGAGAGTTGACTTGAATGCAGGATTGAGGTTATTGATCTTAGAAATCCACGTAGCATAGCAAAAATTCTTGGGACCTTGATATCCTTAGCTGGTGTTTTCACCATGACACTATACAAGGGACCTGTTATGAGAAACTTGTGGCATCCTCTAATCCATATTATTCAACCAAAAAGTTCTTCTATCAACGAGAGTGGATTAAAGGGTTCAATTCTTACTGTCTCATGTTGTGCCACGTTTTCTATATGGTACATTATGCAG

Protein sequence

>MS.gene53287.t1

MALNLYFASLKYTSPTFLASMFNTIASITFIIAVALRIEVIDLRNPRSIAKILGTLISLAGVFTMTLYKGPVMRNLWHPLIHIIQPKSSSINESGLKGSILTVSCCATFSIWYIMQ