Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53373.t1 | RHN75196.1 | 91.8 | 61 | 5 | 0 | 34 | 94 | 498 | 558 | 6.10E-19 | 104 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53373.t1 | A0A072VBM0 | 91.8 | 61 | 5 | 0 | 34 | 94 | 498 | 558 | 4.4e-19 | 104.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050556 | MS.gene53373 | 0.806668 | 7.36E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene53373 | 0.803901 | 2.79E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene53373 | 0.805166 | 1.52E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene53373 | 0.800459 | 1.43E-48 | -1.69E-46 |
| MS.gene056831 | MS.gene53373 | 0.811299 | 7.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53373.t1 | MTR_2g081100 | 90.909 | 66 | 6 | 0 | 34 | 99 | 498 | 563 | 8.41e-32 | 119 |
| MS.gene53373.t1 | MTR_2g081100 | 90.909 | 66 | 6 | 0 | 34 | 99 | 498 | 563 | 1.13e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAGGCTTGGTTTCATTA+AGG | 0.279842 | 4.3:+12459772 | MS.gene53373:CDS |
| AGTTGAACTATGCGACATTA+AGG | 0.318921 | 4.3:+12459756 | MS.gene53373:CDS |
| CACGATATTAGTCTTCTCTT+TGG | 0.320611 | 4.3:+12460431 | MS.gene53373:CDS |
| CCTTTCCGACCTCTGGTGCT+TGG | 0.384030 | 4.3:-12460380 | None:intergenic |
| TCTTGGTGCTCAGTCCTTTG+AGG | 0.398508 | 4.3:+12460140 | MS.gene53373:CDS |
| AACAGATCCTTTCCGACCTC+TGG | 0.421775 | 4.3:-12460387 | None:intergenic |
| ACGCAGCTCCTGCCATGGTC+AGG | 0.431291 | 4.3:+12460683 | MS.gene53373:intron |
| AGTGAATTGTGAAGATTCCT+TGG | 0.446636 | 4.3:-12460118 | None:intergenic |
| TCATCATCGCTGAAGGAAAC+CGG | 0.458878 | 4.3:-12460329 | None:intergenic |
| CACAGAAGCCTGACCATGGC+AGG | 0.462409 | 4.3:-12460691 | None:intergenic |
| ATCACTTTCATTGATTATTG+TGG | 0.462962 | 4.3:+12460743 | MS.gene53373:CDS |
| GAATCTTCACAATTCACTCT+TGG | 0.470201 | 4.3:+12460123 | MS.gene53373:CDS |
| TGCAGCTGGAGCTGATAGTC+CGG | 0.486818 | 4.3:+12460310 | MS.gene53373:CDS |
| TTTGTTCTAGGAACGTGTTA+AGG | 0.492658 | 4.3:+12460080 | MS.gene53373:intron |
| AACTATGCGACATTAAGGCT+TGG | 0.498825 | 4.3:+12459761 | MS.gene53373:CDS |
| TTTCAAGGCAGAAGTGCAGC+TGG | 0.505124 | 4.3:+12460296 | MS.gene53373:CDS |
| ATTTGTTGGGTTTACCTCAA+AGG | 0.513130 | 4.3:-12460154 | None:intergenic |
| GGAGCGATCTACGCGCTCCA+AGG | 0.554334 | 4.3:+12460101 | MS.gene53373:CDS |
| TTTCAACGCAGCTCCTGCCA+TGG | 0.567402 | 4.3:+12460678 | MS.gene53373:intron |
| GGCATCTTCATCATCGCTGA+AGG | 0.569407 | 4.3:-12460336 | None:intergenic |
| CCAAGCACCAGAGGTCGGAA+AGG | 0.579149 | 4.3:+12460380 | MS.gene53373:CDS |
| AGAAGCTAACAAATCTGCAA+AGG | 0.609630 | 4.3:-12460466 | None:intergenic |
| AGCTCAAAACCAAGCACCAG+AGG | 0.612912 | 4.3:+12460371 | MS.gene53373:CDS |
| AGAGAAGACTAATATCGTGA+TGG | 0.628417 | 4.3:-12460427 | None:intergenic |
| CAAAACCAAGCACCAGAGGT+CGG | 0.635046 | 4.3:+12460375 | MS.gene53373:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTATAATATCATTTGTT+GGG | - | chr4.3:12460170-12460189 | None:intergenic | 15.0% |
| !! | TAATTGAAAGAGAAAAAAAA+AGG | - | chr4.3:12460509-12460528 | None:intergenic | 15.0% |
| !! | TGAAAATTATGTAAAAAGTA+GGG | + | chr4.3:12460209-12460228 | MS.gene53373:intron | 15.0% |
| !! | TTGAAAATTATGTAAAAAGT+AGG | + | chr4.3:12460208-12460227 | MS.gene53373:intron | 15.0% |
| !! | CAATGTATAATATCATTTGT+TGG | - | chr4.3:12460171-12460190 | None:intergenic | 20.0% |
| !! | TTGTCATCAGAATTATTTAA+TGG | + | chr4.3:12459812-12459831 | MS.gene53373:intron | 20.0% |
| !!! | TCATTTTTCAAGCAATTTTA+TGG | + | chr4.3:12459722-12459741 | MS.gene53373:CDS | 20.0% |
| !!! | TTGTGATCAGAATTATTTTA+TGG | + | chr4.3:12460019-12460038 | MS.gene53373:intron | 20.0% |
| ! | AAGAAATAAGCTAAGAATGA+AGG | - | chr4.3:12460261-12460280 | None:intergenic | 25.0% |
| ! | ATCATCATCATAACTGTTAA+TGG | + | chr4.3:12460643-12460662 | MS.gene53373:intron | 25.0% |
| !! | ATCACTTTCATTGATTATTG+TGG | + | chr4.3:12460743-12460762 | MS.gene53373:CDS | 25.0% |
| !! | TATGTAAAAAGTAGGGTTTT+GGG | + | chr4.3:12460216-12460235 | MS.gene53373:intron | 25.0% |
| !! | TTATGTAAAAAGTAGGGTTT+TGG | + | chr4.3:12460215-12460234 | MS.gene53373:intron | 25.0% |
| !! | TTATTTCTTTATTCCGTACA+GGG | + | chr4.3:12460272-12460291 | MS.gene53373:intron | 25.0% |
| AAAGCCAACAAATGACATTA+AGG | + | chr4.3:12459963-12459982 | MS.gene53373:intron | 30.0% | |
| AAGAGAAAAAAAAAGGTGAG+AGG | - | chr4.3:12460502-12460521 | None:intergenic | 30.0% | |
| ! | ATCGTTCTTCTTTTTGTTCT+AGG | + | chr4.3:12460068-12460087 | MS.gene53373:intron | 30.0% |
| ! | ATTAAGGCTTGGTTTCATTA+AGG | + | chr4.3:12459772-12459791 | MS.gene53373:CDS | 30.0% |
| ! | CTTATTTCTTTATTCCGTAC+AGG | + | chr4.3:12460271-12460290 | MS.gene53373:intron | 30.0% |
| ACGTAAGTATGATCAATGAC+TGG | + | chr4.3:12459846-12459865 | MS.gene53373:intron | 35.0% | |
| AGAAGCTAACAAATCTGCAA+AGG | - | chr4.3:12460469-12460488 | None:intergenic | 35.0% | |
| AGAATAGTATGATCAGTGAC+TGG | + | chr4.3:12460044-12460063 | MS.gene53373:intron | 35.0% | |
| AGAGAAGACTAATATCGTGA+TGG | - | chr4.3:12460430-12460449 | None:intergenic | 35.0% | |
| AGTGAACTGTGAATATTCCT+TGG | - | chr4.3:12459920-12459939 | None:intergenic | 35.0% | |
| AGTGAATTGTGAAGATTCCT+TGG | - | chr4.3:12460121-12460140 | None:intergenic | 35.0% | |
| AGTTGAACTATGCGACATTA+AGG | + | chr4.3:12459756-12459775 | MS.gene53373:CDS | 35.0% | |
| ATTTATGAGCGTTATGAGAC+TGG | + | chr4.3:12460556-12460575 | MS.gene53373:intron | 35.0% | |
| CAACAAATGACATTAAGGCT+TGG | + | chr4.3:12459968-12459987 | MS.gene53373:intron | 35.0% | |
| GAATCTTCACAATTCACTCT+TGG | + | chr4.3:12460123-12460142 | MS.gene53373:CDS | 35.0% | |
| ! | ATTTGTTGGGTTTACCTCAA+AGG | - | chr4.3:12460157-12460176 | None:intergenic | 35.0% |
| ! | CAAGCCTTAATGTCATTTGT+TGG | - | chr4.3:12459970-12459989 | None:intergenic | 35.0% |
| ! | GGTAACGTTCTTTTTGTTCT+AGG | + | chr4.3:12459867-12459886 | MS.gene53373:intron | 35.0% |
| !! | ATTTGTTGGCTTTACCTCAA+AGG | - | chr4.3:12459956-12459975 | None:intergenic | 35.0% |
| !! | CACGATATTAGTCTTCTCTT+TGG | + | chr4.3:12460431-12460450 | MS.gene53373:CDS | 35.0% |
| !! | TTTGTTCTAGGAACGTGTTA+AGG | + | chr4.3:12459879-12459898 | MS.gene53373:intron | 35.0% |
| AACTATGCGACATTAAGGCT+TGG | + | chr4.3:12459761-12459780 | MS.gene53373:CDS | 40.0% | |
| AGACTGGTGTACATCTATGA+TGG | + | chr4.3:12460572-12460591 | MS.gene53373:intron | 40.0% | |
| GAATATTCACAGTTCACTCC+TGG | + | chr4.3:12459922-12459941 | MS.gene53373:intron | 40.0% | |
| ! | TATTCCGTACAGGGTTTTCA+AGG | + | chr4.3:12460281-12460300 | MS.gene53373:intron | 40.0% |
| ! | TTTTGAGCTTGATGTTGGCA+TGG | - | chr4.3:12460360-12460379 | None:intergenic | 40.0% |
| !!! | CTTGGTTTTGAGCTTGATGT+TGG | - | chr4.3:12460365-12460384 | None:intergenic | 40.0% |
| !!! | TAAAAAGTAGGGTTTTGGGC+AGG | + | chr4.3:12460220-12460239 | MS.gene53373:intron | 40.0% |
| TCATCATCGCTGAAGGAAAC+CGG | - | chr4.3:12460332-12460351 | None:intergenic | 45.0% | |
| TCTGCCTTGAAAACCCTGTA+CGG | - | chr4.3:12460288-12460307 | None:intergenic | 45.0% | |
| ! | TTTTCACAGAAGCCTGACCA+TGG | - | chr4.3:12460698-12460717 | None:intergenic | 45.0% |
| AACAGATCCTTTCCGACCTC+TGG | - | chr4.3:12460390-12460409 | None:intergenic | 50.0% | |
| AGCTCAAAACCAAGCACCAG+AGG | + | chr4.3:12460371-12460390 | MS.gene53373:CDS | 50.0% | |
| CAAAACCAAGCACCAGAGGT+CGG | + | chr4.3:12460375-12460394 | MS.gene53373:CDS | 50.0% | |
| GGCATCTTCATCATCGCTGA+AGG | - | chr4.3:12460339-12460358 | None:intergenic | 50.0% | |
| ! | TTTCAAGGCAGAAGTGCAGC+TGG | + | chr4.3:12460296-12460315 | MS.gene53373:CDS | 50.0% |
| !! | TCTTGGTGCTCAGTCCTTTG+AGG | + | chr4.3:12460140-12460159 | MS.gene53373:CDS | 50.0% |
| ACCTCAAAGGACTGAGCACC+AGG | - | chr4.3:12459943-12459962 | None:intergenic | 55.0% | |
| TCCTGGTGCTCAGTCCTTTG+AGG | + | chr4.3:12459939-12459958 | MS.gene53373:intron | 55.0% | |
| TGCAGCTGGAGCTGATAGTC+CGG | + | chr4.3:12460310-12460329 | MS.gene53373:CDS | 55.0% | |
| TTTCAACGCAGCTCCTGCCA+TGG | + | chr4.3:12460678-12460697 | MS.gene53373:intron | 55.0% | |
| CACAGAAGCCTGACCATGGC+AGG | - | chr4.3:12460694-12460713 | None:intergenic | 60.0% | |
| CCAAGCACCAGAGGTCGGAA+AGG | + | chr4.3:12460380-12460399 | MS.gene53373:CDS | 60.0% | |
| CCTTTCCGACCTCTGGTGCT+TGG | - | chr4.3:12460383-12460402 | None:intergenic | 60.0% | |
| GGAGTGATCGACACGCTCCA+AGG | + | chr4.3:12459900-12459919 | MS.gene53373:intron | 60.0% | |
| ACGCAGCTCCTGCCATGGTC+AGG | + | chr4.3:12460683-12460702 | MS.gene53373:intron | 65.0% | |
| GGAGCGATCTACGCGCTCCA+AGG | + | chr4.3:12460101-12460120 | MS.gene53373:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 12459692 | 12460775 | 12459692 | ID=MS.gene53373 |
| chr4.3 | mRNA | 12459692 | 12460775 | 12459692 | ID=MS.gene53373.t1;Parent=MS.gene53373 |
| chr4.3 | exon | 12459692 | 12459793 | 12459692 | ID=MS.gene53373.t1.exon1;Parent=MS.gene53373.t1 |
| chr4.3 | CDS | 12459692 | 12459793 | 12459692 | ID=cds.MS.gene53373.t1;Parent=MS.gene53373.t1 |
| chr4.3 | exon | 12460090 | 12460161 | 12460090 | ID=MS.gene53373.t1.exon2;Parent=MS.gene53373.t1 |
| chr4.3 | CDS | 12460090 | 12460161 | 12460090 | ID=cds.MS.gene53373.t1;Parent=MS.gene53373.t1 |
| chr4.3 | exon | 12460293 | 12460478 | 12460293 | ID=MS.gene53373.t1.exon3;Parent=MS.gene53373.t1 |
| chr4.3 | CDS | 12460293 | 12460478 | 12460293 | ID=cds.MS.gene53373.t1;Parent=MS.gene53373.t1 |
| chr4.3 | exon | 12460689 | 12460775 | 12460689 | ID=MS.gene53373.t1.exon4;Parent=MS.gene53373.t1 |
| chr4.3 | CDS | 12460689 | 12460775 | 12460689 | ID=cds.MS.gene53373.t1;Parent=MS.gene53373.t1 |
| Gene Sequence |
| Protein sequence |
