AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53559

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53559.t1 MTR_1g045550 98.807 503 6 0 1 503 1 503 0.0 1013
MS.gene53559.t1 MTR_7g098930 74.948 483 119 2 1 482 1 482 0.0 736
MS.gene53559.t1 MTR_1g079760 74.359 507 118 5 1 498 1 504 0.0 734
MS.gene53559.t1 MTR_7g113340 73.444 482 126 2 1 481 1 481 0.0 719
MS.gene53559.t1 MTR_3g463730 66.372 452 147 4 45 493 11 460 0.0 594
MS.gene53559.t1 MTR_7g115050 27.007 411 239 13 113 501 100 471 1.92e-23 103
MS.gene53559.t1 MTR_1g036410 27.097 465 272 16 48 482 24 451 1.26e-22 101
MS.gene53559.t1 MTR_8g074750 25.979 485 295 15 48 502 30 480 4.84e-20 93.2
MS.gene53559.t1 MTR_7g069640 26.638 473 280 16 48 485 22 462 1.38e-18 89.0
MS.gene53559.t1 MTR_8g095040 23.958 480 305 11 48 496 29 479 1.05e-16 83.2
MS.gene53559.t1 MTR_4g017820 26.971 482 281 16 48 495 22 466 3.50e-15 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53559.t1 AT4G13510 80.478 502 88 3 5 499 2 500 0.0 828
MS.gene53559.t1 AT3G24300 77.465 497 99 4 6 497 7 495 0.0 779
MS.gene53559.t1 AT3G24290 79.237 472 95 1 6 477 7 475 0.0 769
MS.gene53559.t1 AT1G64780 76.190 483 104 7 27 500 31 511 0.0 738
MS.gene53559.t1 AT4G28700 74.745 491 116 4 5 489 6 494 0.0 712
MS.gene53559.t1 AT2G38290 27.233 459 283 13 48 485 20 448 5.45e-23 102
MS.gene53559.t1 AT2G38290 27.809 356 221 10 136 485 7 332 1.41e-18 87.8

Find 154 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACCCTATGGGTTATTTA+TGG 0.176472 1.2:-28470547 MS.gene53559:CDS
GGCACATGTGATACAAATTT+TGG 0.187812 1.2:-28470502 MS.gene53559:CDS
TGGTGGTATTGCTGGTCTTT+GGG 0.199248 1.2:-28471238 MS.gene53559:CDS
AGTGCTTCTCTTGTTGTTCT+TGG 0.212206 1.2:-28471144 MS.gene53559:CDS
CCAAGTTTATCCGGGTAAAC+TGG 0.240628 1.2:-28470571 MS.gene53559:CDS
TTGGTGGTATTGCTGGTCTT+TGG 0.246084 1.2:-28471239 MS.gene53559:CDS
CTGTGGTTTCTCATTGGTTC+TGG 0.264221 1.2:-28471356 MS.gene53559:CDS
ATTCTCAGTCCTTATAATTC+AGG 0.269091 1.2:-28471066 MS.gene53559:intron
GCTTCGGCGTTGGTGGCGTT+GGG 0.277122 1.2:+28471828 None:intergenic
GGTGGTGGAGGGAAATTATT+AGG 0.301790 1.2:-28470525 MS.gene53559:CDS
GGCTTCGGCGTTGGTGGCGT+TGG 0.324200 1.2:+28471827 None:intergenic
ACACCTTCAAATGGTTTCAT+TGG 0.325076 1.2:-28471555 MS.gene53559:CDS
CATATGGTTGGTGGTATTGC+TGG 0.332447 1.2:-28471246 MS.gene53559:CDS
GATGAAATTTGCGGCGGCTT+CGG 0.336367 1.2:+28471812 None:intergenic
CTTATTGAAGGTCCGCGAAT+TGG 0.341909 1.2:-28471210 MS.gene53559:CDS
TTCTTGGTACCTTCATGCTT+TGG 0.347565 1.2:-28471128 MS.gene53559:CDS
TTTACCCTGTGGTTTCTCAT+TGG 0.351616 1.2:-28471362 MS.gene53559:CDS
CGACCCTATGGGTTATTTAT+GGG 0.352551 1.2:-28470546 MS.gene53559:CDS
GATGAAGGATCACATAGAAA+TGG 0.373243 1.2:-28470339 MS.gene53559:CDS
TGTAATTGAGCTGCTTCTAA+TGG 0.376380 1.2:+28470657 None:intergenic
TGGGTGGGTAAGTGCAACTA+TGG 0.381238 1.2:-28470472 MS.gene53559:CDS
TACAATTACAAATCTGTAGT+TGG 0.383920 1.2:+28470270 None:intergenic
CCTTCATGCTTTGGTTTGGT+TGG 0.392626 1.2:-28471119 MS.gene53559:CDS
TGGCTCCGGTGTTGTTCATA+TGG 0.393312 1.2:-28471262 MS.gene53559:CDS
TTCATTGGAAAACATTTCTT+CGG 0.397824 1.2:-28471540 MS.gene53559:CDS
GGTAAACTGGGTCGACCCTA+TGG 0.406965 1.2:-28470558 MS.gene53559:CDS
GTATGTGAACCAAGTTTATC+CGG 0.416107 1.2:-28470580 MS.gene53559:CDS
CTTTGGGGAGCACTTATTGA+AGG 0.417245 1.2:-28471222 MS.gene53559:CDS
GGTACCTTCATGCTTTGGTT+TGG 0.417688 1.2:-28471123 MS.gene53559:CDS
TCGCAGCTCGCGCAGTTCAT+CGG 0.418885 1.2:-28471852 MS.gene53559:CDS
TGAAGATGAACTTGCGGGTA+TGG 0.421273 1.2:-28470400 MS.gene53559:CDS
AGTTAACTACCTGAATTATA+AGG 0.425481 1.2:+28471057 None:intergenic
TCAACTGAAGATGAACTTGC+GGG 0.428559 1.2:-28470405 MS.gene53559:CDS
AAAGAATTCTCTCCGGTCAC+TGG 0.431120 1.2:-28470839 MS.gene53559:CDS
CCAGTTTACCCGGATAAACT+TGG 0.434575 1.2:+28470571 None:intergenic
ATGCTTTGGTTTGGTTGGTA+TGG 0.439074 1.2:-28471114 MS.gene53559:CDS
GCCTTTGGAACACCTTCAAA+TGG 0.441214 1.2:-28471564 MS.gene53559:CDS
AGAATCTTGGTGAAGGAACC+AGG 0.441335 1.2:+28471084 None:intergenic
ATTTGCGGCGGCTTCGGCGT+TGG 0.442397 1.2:+28471818 None:intergenic
GGTTCTGGTCCGGTGATGGT+TGG 0.442491 1.2:-28471341 MS.gene53559:CDS
TACACGGTGGGTGTGGCGCG+TGG 0.444248 1.2:-28470638 MS.gene53559:CDS
GGTTGGTATGGTTTCAACCC+TGG 0.447548 1.2:-28471102 MS.gene53559:CDS
GGGTTATTTATGGGTGGTGG+AGG 0.451374 1.2:-28470537 MS.gene53559:CDS
GTTCTGGTCCGGTGATGGTT+GGG 0.451505 1.2:-28471340 MS.gene53559:CDS
CATTGGTTCTGGTCCGGTGA+TGG 0.453436 1.2:-28471345 MS.gene53559:CDS
TTTCCAATGAAACCATTTGA+AGG 0.457318 1.2:+28471552 None:intergenic
GTAAGCTGAAAATAGAAGAT+AGG 0.461570 1.2:+28471728 None:intergenic
TGCGGCGGCTTCGGCGTTGG+TGG 0.461817 1.2:+28471821 None:intergenic
GGTTTCGCCATGCTTTGTGC+TGG 0.462477 1.2:-28471684 MS.gene53559:CDS
TATGGGTTATTTATGGGTGG+TGG 0.467977 1.2:-28470540 MS.gene53559:CDS
TTATTCAAAACAAAGAATAG+TGG 0.471377 1.2:+28470447 None:intergenic
ATGAAGGATCACATAGAAAT+GGG 0.473794 1.2:-28470338 MS.gene53559:CDS
AACGTCCTCGACGCTGCTGC+TGG 0.476966 1.2:-28471621 MS.gene53559:CDS
GGTGGTATTGCTGGTCTTTG+GGG 0.480605 1.2:-28471237 MS.gene53559:CDS
ATAAGCAAACCCACCATGTC+GGG 0.481927 1.2:+28470371 None:intergenic
ACACGGTGGGTGTGGCGCGT+GGG 0.483461 1.2:-28470637 MS.gene53559:CDS
ATGGAGTGCGGTGGGGAGGA+CGG 0.485440 1.2:-28470916 MS.gene53559:CDS
ATTTACTCATCGTTTCTCAC+AGG 0.487248 1.2:-28471390 MS.gene53559:CDS
TCCGGTGTTGTTCATATGGT+TGG 0.488732 1.2:-28471258 MS.gene53559:CDS
CACGGTGGGTGTGGCGCGTG+GGG 0.489854 1.2:-28470636 MS.gene53559:CDS
GCTTATGCTTATGAAGATGA+AGG 0.489963 1.2:-28470354 MS.gene53559:CDS
GTTTCTCATTGGTTCTGGTC+CGG 0.495880 1.2:-28471351 MS.gene53559:CDS
ATTATAAGGACTGAGAATCT+TGG 0.495971 1.2:+28471071 None:intergenic
TGAATAAATTGAAGTTGTTG+AGG 0.497576 1.2:-28470431 MS.gene53559:CDS
TAGGTACTTATTATGGGCAA+TGG 0.500219 1.2:-28470935 MS.gene53559:CDS
AAAACATTTCTTCGGTCTGA+AGG 0.500891 1.2:-28471532 MS.gene53559:CDS
GGTCGAGCCGTGGCACTTCG+CGG 0.501540 1.2:-28471171 MS.gene53559:CDS
GGCAATGGAGTGCGGTGGGG+AGG 0.501855 1.2:-28470920 MS.gene53559:CDS
GTGGTGGAGGGAAATTATTA+GGG 0.501970 1.2:-28470524 MS.gene53559:CDS
TATGGGCAATGGAGTGCGGT+GGG 0.503029 1.2:-28470924 MS.gene53559:CDS
GACGTCTGCAACGGATTACT+CGG 0.505173 1.2:-28470807 MS.gene53559:CDS
AAACAAAAGGTCCCCGGTGT+TGG 0.506596 1.2:+28471308 None:intergenic
TGACTCCGGTGCCAAACAAA+AGG 0.510137 1.2:+28471295 None:intergenic
GTCGGTCACGTTCCAGTGAC+CGG 0.510750 1.2:+28470827 None:intergenic
TGGGCTAGCGCAACCAACAC+CGG 0.511581 1.2:-28471321 MS.gene53559:CDS
GCGGTGACGACGACATTAGC+AGG 0.511677 1.2:-28470894 MS.gene53559:CDS
CAAGTTTATCCGGGTAAACT+GGG 0.514789 1.2:-28470570 MS.gene53559:CDS
AGCTGCGAAACAGAGCATTC+CGG 0.515010 1.2:+28471867 None:intergenic
TTCGGGAAAAGAATTCTCTC+CGG 0.517363 1.2:-28470846 MS.gene53559:CDS
TTGAAGGTCCGCGAATTGGA+CGG 0.518069 1.2:-28471206 MS.gene53559:CDS
GTTGGTAAGCATGATGTTCA+TGG 0.520266 1.2:+28471641 None:intergenic
GCTCAATTACACGGTGGGTG+TGG 0.523195 1.2:-28470645 MS.gene53559:CDS
TGCGCTAGCCCAACCATCAC+CGG 0.524846 1.2:+28471332 None:intergenic
GGTTATTTATGGGTGGTGGA+GGG 0.528393 1.2:-28470536 MS.gene53559:CDS
GGGCTAGCGCAACCAACACC+GGG 0.530553 1.2:-28471320 MS.gene53559:CDS
CCCACCAAACATGTCGCTGC+CGG 0.531903 1.2:-28471886 None:intergenic
GGTGTTCCGTTGTAGAACCA+TGG 0.539046 1.2:-28470761 MS.gene53559:CDS
TGGTTCTACAACGGAACACC+CGG 0.540665 1.2:+28470764 None:intergenic
TGATCGAACCGTCCAATTCG+CGG 0.541228 1.2:+28471198 None:intergenic
CCCTATGGGTTATTTATGGG+TGG 0.544513 1.2:-28470543 MS.gene53559:CDS
TTTACTCATCGTTTCTCACA+GGG 0.546630 1.2:-28471389 MS.gene53559:CDS
ATCTGTAGTTGGAGTAGGAG+TGG 0.549034 1.2:+28470281 None:intergenic
TCGTTCCGCTATAGAGCCGC+TGG 0.552932 1.2:+28471434 None:intergenic
ATAGAAATGGGACTCAATTG+AGG 0.554439 1.2:-28470326 MS.gene53559:CDS
ACCATTTGAAGGTGTTCCAA+AGG 0.565904 1.2:+28471563 None:intergenic
GTGTTCCGTTGTAGAACCAT+GGG 0.568191 1.2:-28470760 MS.gene53559:CDS
GTCCTCGACGCTGCTGCTGG+TGG 0.568629 1.2:-28471618 MS.gene53559:CDS
GTGTAACAAACTCGCGGAGA+AGG 0.568835 1.2:-28470694 MS.gene53559:CDS
CAAGTTGCATAGAGAAAACA+AGG 0.569214 1.2:+28471706 None:intergenic
CTTCGGCGTTGGTGGCGTTG+GGG 0.570819 1.2:+28471829 None:intergenic
TGAAGGTGTTCCAAAGGCAA+AGG 0.570919 1.2:+28471569 None:intergenic
CACAGGGTTTGTTTACCCTG+TGG 0.571684 1.2:-28471373 MS.gene53559:CDS
AACTGAACCAGCACAAAGCA+TGG 0.577901 1.2:+28471677 None:intergenic
TTCAACTGAAGATGAACTTG+CGG 0.585243 1.2:-28470406 MS.gene53559:CDS
GGTGTTGTTCATATGGTTGG+TGG 0.587357 1.2:-28471255 MS.gene53559:CDS
GTAAACTGGGTCGACCCTAT+GGG 0.589828 1.2:-28470557 MS.gene53559:CDS
ATTGGACGGTTCGATCACTC+TGG 0.590544 1.2:-28471192 MS.gene53559:CDS
TAGGGTCGACCCAGTTTACC+CGG 0.590994 1.2:+28470561 None:intergenic
ACTATGTCCGCGAAGTGCCA+CGG 0.591071 1.2:+28471164 None:intergenic
AACGTGACCGACGTCTGCAA+CGG 0.591243 1.2:-28470816 MS.gene53559:CDS
CCACATACTATTGCTGCCCA+TGG 0.592786 1.2:+28470744 None:intergenic
CCATGGGCAGCAATAGTATG+TGG 0.592800 1.2:-28470744 MS.gene53559:CDS
TACAAATCTGTAGTTGGAGT+AGG 0.593435 1.2:+28470276 None:intergenic
GCCAGCAAAATCAATGACTC+CGG 0.596809 1.2:+28471281 None:intergenic
GACGACATTAGCAGGTTGCA+CGG 0.597957 1.2:-28470886 MS.gene53559:CDS
GGGTGGGTAAGTGCAACTAT+GGG 0.598468 1.2:-28470471 MS.gene53559:CDS
TGGATTTGACCCGACATGGT+GGG 0.598703 1.2:-28470380 MS.gene53559:CDS
GGTGCCAAACAAAAGGTCCC+CGG 0.599775 1.2:+28471302 None:intergenic
CATAAGCAAACCCACCATGT+CGG 0.601038 1.2:+28470370 None:intergenic
CAGAACCAATGAGAAACCAC+AGG 0.603230 1.2:+28471357 None:intergenic
CCAACCAAACCAAAGCATGA+AGG 0.603979 1.2:+28471119 None:intergenic
CGATCACTCTGGTCGAGCCG+TGG 0.608129 1.2:-28471181 MS.gene53559:CDS
ACCACCCATAAATAACCCAT+AGG 0.609604 1.2:+28470542 None:intergenic
TTATGGGCAATGGAGTGCGG+TGG 0.609738 1.2:-28470925 MS.gene53559:CDS
TATGTGAACCAAGTTTATCC+GGG 0.612424 1.2:-28470579 MS.gene53559:CDS
GAGTGCGGTGGGGAGGACGG+CGG 0.614067 1.2:-28470913 MS.gene53559:CDS
CTGAGTGCAGATGAAATTTG+CGG 0.614324 1.2:+28471803 None:intergenic
AAGCAGCTCAATTACACGGT+GGG 0.620203 1.2:-28470650 MS.gene53559:CDS
CGCCACCAGCAGCAGCGTCG+AGG 0.620566 1.2:+28471616 None:intergenic
AGGACTGAGAATCTTGGTGA+AGG 0.622311 1.2:+28471077 None:intergenic
CTCTTCTTTCGCAAACAAAG+CGG 0.625082 1.2:+28470602 None:intergenic
CCACCCATAAATAACCCATA+GGG 0.625187 1.2:+28470543 None:intergenic
GGTATGGATTTGACCCGACA+TGG 0.625832 1.2:-28470384 MS.gene53559:CDS
AGCAGCAGCGTCGAGGACGT+TGG 0.631989 1.2:+28471623 None:intergenic
TATTACCAGCGGCTCTATAG+CGG 0.632555 1.2:-28471439 MS.gene53559:CDS
ATGGATTTGACCCGACATGG+TGG 0.636723 1.2:-28470381 MS.gene53559:CDS
TGGTGGAGGGAAATTATTAG+GGG 0.638481 1.2:-28470523 MS.gene53559:CDS
ACCAACCATATGAACAACAC+CGG 0.643450 1.2:+28471257 None:intergenic
TGCTGCCCATGGTTCTACAA+CGG 0.652613 1.2:+28470755 None:intergenic
AGTGCAGATGAAATTTGCGG+CGG 0.665787 1.2:+28471806 None:intergenic
GAGTAATCCGTTGCAGACGT+CGG 0.670118 1.2:+28470809 None:intergenic
ATGGGCAATGGAGTGCGGTG+GGG 0.682220 1.2:-28470923 MS.gene53559:CDS
TTATTATGGGCAATGGAGTG+CGG 0.683210 1.2:-28470928 MS.gene53559:CDS
GAATCTTGGTGAAGGAACCA+GGG 0.685698 1.2:+28471085 None:intergenic
GCAGCCGCTGGTATTACCAG+CGG 0.694342 1.2:-28471450 MS.gene53559:CDS
TTAGAAGCAGCTCAATTACA+CGG 0.700908 1.2:-28470654 MS.gene53559:CDS
GTCTGCAACGGATTACTCGG+CGG 0.702672 1.2:-28470804 MS.gene53559:CDS
AATCGCGTGTAACAAACTCG+CGG 0.719057 1.2:-28470700 MS.gene53559:CDS
AGAGCCGCTGGTAATACCAG+CGG 0.726819 1.2:+28471446 None:intergenic
TTCTACAACGGAACACCCGG+CGG 0.739650 1.2:+28470767 None:intergenic
GAAGCAGCTCAATTACACGG+TGG 0.741494 1.2:-28470651 MS.gene53559:CDS
GGCTAGCGCAACCAACACCG+GGG 0.741776 1.2:-28471319 MS.gene53559:CDS
ACACGCGATTAAAACAATAG+CGG 0.752803 1.2:+28470713 None:intergenic
AGAACCAATGAGAAACCACA+GGG 0.754327 1.2:+28471358 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTTTTATGCAATTTT+AGG - chr1.2:28471197-28471216 MS.gene53559:CDS 10.0%
!!! ATACAAATTTTGGTTATTAT+TGG - chr1.2:28471659-28471678 MS.gene53559:CDS 15.0%
!!! TACAAATTTTGGTTATTATT+GGG - chr1.2:28471660-28471679 MS.gene53559:CDS 15.0%
!! TTATTCAAAACAAAGAATAG+TGG + chr1.2:28471707-28471726 None:intergenic 20.0%
!!! CAATTTTAGGTACTTATTAT+GGG - chr1.2:28471210-28471229 MS.gene53559:CDS 20.0%
! AGTTAACTACCTGAATTATA+AGG + chr1.2:28471097-28471116 None:intergenic 25.0%
! ATTGTTTGGATGCATTAAAA+TGG - chr1.2:28471144-28471163 MS.gene53559:CDS 25.0%
! TACTAACTACTTTCATTGTT+TGG - chr1.2:28471130-28471149 MS.gene53559:CDS 25.0%
! TGAATAAATTGAAGTTGTTG+AGG - chr1.2:28471720-28471739 MS.gene53559:CDS 25.0%
! TTCATTGGAAAACATTTCTT+CGG - chr1.2:28470611-28470630 MS.gene53559:CDS 25.0%
!! CTGTAATCAAAATTTTCAGT+CGG + chr1.2:28470650-28470669 None:intergenic 25.0%
!!! AAATTTTGGTTATTATTGGG+TGG - chr1.2:28471663-28471682 MS.gene53559:CDS 25.0%
!!! AATTTTGGTTATTATTGGGT+GGG - chr1.2:28471664-28471683 MS.gene53559:CDS 25.0%
!!! GCAATTTTAGGTACTTATTA+TGG - chr1.2:28471209-28471228 MS.gene53559:CDS 25.0%
ATGAAGGATCACATAGAAAT+GGG - chr1.2:28471813-28471832 MS.gene53559:CDS 30.0%
ATTATAAGGACTGAGAATCT+TGG + chr1.2:28471083-28471102 None:intergenic 30.0%
ATTCTCAGTCCTTATAATTC+AGG - chr1.2:28471085-28471104 MS.gene53559:CDS 30.0%
GTAAGCTGAAAATAGAAGAT+AGG + chr1.2:28470426-28470445 None:intergenic 30.0%
TTTCCAATGAAACCATTTGA+AGG + chr1.2:28470602-28470621 None:intergenic 30.0%
!! ACAGCTATTTTCTCTATCAA+TGG - chr1.2:28470666-28470685 MS.gene53559:CDS 30.0%
!! CAGCTATTTTCTCTATCAAT+GGG - chr1.2:28470667-28470686 MS.gene53559:CDS 30.0%
AAAACATTTCTTCGGTCTGA+AGG - chr1.2:28470619-28470638 MS.gene53559:CDS 35.0%
ACACCTTCAAATGGTTTCAT+TGG - chr1.2:28470596-28470615 MS.gene53559:CDS 35.0%
ACACGCGATTAAAACAATAG+CGG + chr1.2:28471441-28471460 None:intergenic 35.0%
ATAGAAATGGGACTCAATTG+AGG - chr1.2:28471825-28471844 MS.gene53559:CDS 35.0%
ATTTACTCATCGTTTCTCAC+AGG - chr1.2:28470761-28470780 MS.gene53559:CDS 35.0%
CAAGTTGCATAGAGAAAACA+AGG + chr1.2:28470448-28470467 None:intergenic 35.0%
GATGAAGGATCACATAGAAA+TGG - chr1.2:28471812-28471831 MS.gene53559:CDS 35.0%
GCTTATGCTTATGAAGATGA+AGG - chr1.2:28471797-28471816 MS.gene53559:CDS 35.0%
GTATGTGAACCAAGTTTATC+CGG - chr1.2:28471571-28471590 MS.gene53559:CDS 35.0%
TACAAATCTGTAGTTGGAGT+AGG + chr1.2:28471878-28471897 None:intergenic 35.0%
TAGGTACTTATTATGGGCAA+TGG - chr1.2:28471216-28471235 MS.gene53559:CDS 35.0%
TATGTGAACCAAGTTTATCC+GGG - chr1.2:28471572-28471591 MS.gene53559:CDS 35.0%
TGTAATTGAGCTGCTTCTAA+TGG + chr1.2:28471497-28471516 None:intergenic 35.0%
TTAGAAGCAGCTCAATTACA+CGG - chr1.2:28471497-28471516 MS.gene53559:CDS 35.0%
TTCAACTGAAGATGAACTTG+CGG - chr1.2:28471745-28471764 MS.gene53559:CDS 35.0%
TTTACTCATCGTTTCTCACA+GGG - chr1.2:28470762-28470781 MS.gene53559:CDS 35.0%
! GGCACATGTGATACAAATTT+TGG - chr1.2:28471649-28471668 MS.gene53559:CDS 35.0%
!! CTTGTTTTCTCTATGCAACT+TGG - chr1.2:28470446-28470465 MS.gene53559:CDS 35.0%
ACCAACCATATGAACAACAC+CGG + chr1.2:28470897-28470916 None:intergenic 40.0%
ACCACCCATAAATAACCCAT+AGG + chr1.2:28471612-28471631 None:intergenic 40.0%
AGAACCAATGAGAAACCACA+GGG + chr1.2:28470796-28470815 None:intergenic 40.0%
CAAGTTTATCCGGGTAAACT+GGG - chr1.2:28471581-28471600 MS.gene53559:CDS 40.0%
CCACCCATAAATAACCCATA+GGG + chr1.2:28471611-28471630 None:intergenic 40.0%
CTCTTCTTTCGCAAACAAAG+CGG + chr1.2:28471552-28471571 None:intergenic 40.0%
CTGAGTGCAGATGAAATTTG+CGG + chr1.2:28470351-28470370 None:intergenic 40.0%
TCAACTGAAGATGAACTTGC+GGG - chr1.2:28471746-28471765 MS.gene53559:CDS 40.0%
TGGTGGAGGGAAATTATTAG+GGG - chr1.2:28471628-28471647 MS.gene53559:CDS 40.0%
TTATTATGGGCAATGGAGTG+CGG - chr1.2:28471223-28471242 MS.gene53559:CDS 40.0%
TTCGGGAAAAGAATTCTCTC+CGG - chr1.2:28471305-28471324 MS.gene53559:CDS 40.0%
TTCTTGGTACCTTCATGCTT+TGG - chr1.2:28471023-28471042 MS.gene53559:intron 40.0%
TTTACCCTGTGGTTTCTCAT+TGG - chr1.2:28470789-28470808 MS.gene53559:CDS 40.0%
! CGACCCTATGGGTTATTTAT+GGG - chr1.2:28471605-28471624 MS.gene53559:CDS 40.0%
! GGCGTTTTCTACTATCTCTT+CGG - chr1.2:28470554-28470573 MS.gene53559:CDS 40.0%
! GTGGTGGAGGGAAATTATTA+GGG - chr1.2:28471627-28471646 MS.gene53559:CDS 40.0%
! GTTGGTAAGCATGATGTTCA+TGG + chr1.2:28470513-28470532 None:intergenic 40.0%
! TATGGGTTATTTATGGGTGG+TGG - chr1.2:28471611-28471630 MS.gene53559:CDS 40.0%
! TCGACCCTATGGGTTATTTA+TGG - chr1.2:28471604-28471623 MS.gene53559:CDS 40.0%
!! ACCATTTGAAGGTGTTCCAA+AGG + chr1.2:28470591-28470610 None:intergenic 40.0%
!! AGTGCTTCTCTTGTTGTTCT+TGG - chr1.2:28471007-28471026 MS.gene53559:intron 40.0%
!! ATGCTTTGGTTTGGTTGGTA+TGG - chr1.2:28471037-28471056 MS.gene53559:intron 40.0%
AAAGAATTCTCTCCGGTCAC+TGG - chr1.2:28471312-28471331 MS.gene53559:CDS 45.0%
AACTGAACCAGCACAAAGCA+TGG + chr1.2:28470477-28470496 None:intergenic 45.0%
AAGCAGCTCAATTACACGGT+GGG - chr1.2:28471501-28471520 MS.gene53559:CDS 45.0%
AATCGCGTGTAACAAACTCG+CGG - chr1.2:28471451-28471470 MS.gene53559:CDS 45.0%
AGAATCTTGGTGAAGGAACC+AGG + chr1.2:28471070-28471089 None:intergenic 45.0%
AGGACTGAGAATCTTGGTGA+AGG + chr1.2:28471077-28471096 None:intergenic 45.0%
AGTGCAGATGAAATTTGCGG+CGG + chr1.2:28470348-28470367 None:intergenic 45.0%
ATAAGCAAACCCACCATGTC+GGG + chr1.2:28471783-28471802 None:intergenic 45.0%
ATCTGTAGTTGGAGTAGGAG+TGG + chr1.2:28471873-28471892 None:intergenic 45.0%
CAGAACCAATGAGAAACCAC+AGG + chr1.2:28470797-28470816 None:intergenic 45.0%
CATAAGCAAACCCACCATGT+CGG + chr1.2:28471784-28471803 None:intergenic 45.0%
CCAACCAAACCAAAGCATGA+AGG + chr1.2:28471035-28471054 None:intergenic 45.0%
CCAAGTTTATCCGGGTAAAC+TGG - chr1.2:28471580-28471599 MS.gene53559:CDS 45.0%
CCAGTTTACCCGGATAAACT+TGG + chr1.2:28471583-28471602 None:intergenic 45.0%
CTTATTGAAGGTCCGCGAAT+TGG - chr1.2:28470941-28470960 MS.gene53559:intron 45.0%
GAATCTTGGTGAAGGAACCA+GGG + chr1.2:28471069-28471088 None:intergenic 45.0%
GCCAGCAAAATCAATGACTC+CGG + chr1.2:28470873-28470892 None:intergenic 45.0%
GGTACCTTCATGCTTTGGTT+TGG - chr1.2:28471028-28471047 MS.gene53559:intron 45.0%
GTGTTCCGTTGTAGAACCAT+GGG - chr1.2:28471391-28471410 MS.gene53559:CDS 45.0%
TATTACCAGCGGCTCTATAG+CGG - chr1.2:28470712-28470731 MS.gene53559:CDS 45.0%
TGAAGATGAACTTGCGGGTA+TGG - chr1.2:28471751-28471770 MS.gene53559:CDS 45.0%
! CCCTATGGGTTATTTATGGG+TGG - chr1.2:28471608-28471627 MS.gene53559:CDS 45.0%
! TTCGGTTTTGCCTTTGCCTT+TGG - chr1.2:28470572-28470591 MS.gene53559:CDS 45.0%
!! CATATGGTTGGTGGTATTGC+TGG - chr1.2:28470905-28470924 MS.gene53559:CDS 45.0%
!! CCTTCATGCTTTGGTTTGGT+TGG - chr1.2:28471032-28471051 MS.gene53559:intron 45.0%
!! CTGTGGTTTCTCATTGGTTC+TGG - chr1.2:28470795-28470814 MS.gene53559:CDS 45.0%
!! CTTTGGGGAGCACTTATTGA+AGG - chr1.2:28470929-28470948 MS.gene53559:CDS 45.0%
!! GCCTTTGGAACACCTTCAAA+TGG - chr1.2:28470587-28470606 MS.gene53559:CDS 45.0%
!! GGTGGTGGAGGGAAATTATT+AGG - chr1.2:28471626-28471645 MS.gene53559:CDS 45.0%
!! GGTGTTGTTCATATGGTTGG+TGG - chr1.2:28470896-28470915 MS.gene53559:CDS 45.0%
!! GGTTATTTATGGGTGGTGGA+GGG - chr1.2:28471615-28471634 MS.gene53559:CDS 45.0%
!! GTTTCTCATTGGTTCTGGTC+CGG - chr1.2:28470800-28470819 MS.gene53559:CDS 45.0%
!! TCCGGTGTTGTTCATATGGT+TGG - chr1.2:28470893-28470912 MS.gene53559:CDS 45.0%
!! TGAAGGTGTTCCAAAGGCAA+AGG + chr1.2:28470585-28470604 None:intergenic 45.0%
!! TGGTGGTATTGCTGGTCTTT+GGG - chr1.2:28470913-28470932 MS.gene53559:CDS 45.0%
!! TTGGTGGTATTGCTGGTCTT+TGG - chr1.2:28470912-28470931 MS.gene53559:CDS 45.0%
!!! ACCGGAGTCATTGATTTTGC+TGG - chr1.2:28470869-28470888 MS.gene53559:CDS 45.0%
!!! GTCATTGATTTTGCTGGCTC+CGG - chr1.2:28470875-28470894 MS.gene53559:CDS 45.0%
AAACAAAAGGTCCCCGGTGT+TGG + chr1.2:28470846-28470865 None:intergenic 50.0%
ATGGATTTGACCCGACATGG+TGG - chr1.2:28471770-28471789 MS.gene53559:CDS 50.0%
ATTGGACGGTTCGATCACTC+TGG - chr1.2:28470959-28470978 MS.gene53559:intron 50.0%
CCACATACTATTGCTGCCCA+TGG + chr1.2:28471410-28471429 None:intergenic 50.0%
CCATGGGCAGCAATAGTATG+TGG - chr1.2:28471407-28471426 MS.gene53559:CDS 50.0%
GAAGCAGCTCAATTACACGG+TGG - chr1.2:28471500-28471519 MS.gene53559:CDS 50.0%
GACGACATTAGCAGGTTGCA+CGG - chr1.2:28471265-28471284 MS.gene53559:CDS 50.0%
GAGTAATCCGTTGCAGACGT+CGG + chr1.2:28471345-28471364 None:intergenic 50.0%
GATGAAATTTGCGGCGGCTT+CGG + chr1.2:28470342-28470361 None:intergenic 50.0%
GGGTGGGTAAGTGCAACTAT+GGG - chr1.2:28471680-28471699 MS.gene53559:CDS 50.0%
GGTATGGATTTGACCCGACA+TGG - chr1.2:28471767-28471786 MS.gene53559:CDS 50.0%
GGTGTTCCGTTGTAGAACCA+TGG - chr1.2:28471390-28471409 MS.gene53559:CDS 50.0%
GTAAACTGGGTCGACCCTAT+GGG - chr1.2:28471594-28471613 MS.gene53559:CDS 50.0%
GTGTAACAAACTCGCGGAGA+AGG - chr1.2:28471457-28471476 MS.gene53559:CDS 50.0%
TGATCGAACCGTCCAATTCG+CGG + chr1.2:28470956-28470975 None:intergenic 50.0%
TGGATTTGACCCGACATGGT+GGG - chr1.2:28471771-28471790 MS.gene53559:CDS 50.0%
TGGGTGGGTAAGTGCAACTA+TGG - chr1.2:28471679-28471698 MS.gene53559:CDS 50.0%
TTGAAGGTCCGCGAATTGGA+CGG - chr1.2:28470945-28470964 MS.gene53559:intron 50.0%
! AACACCGGGGACCTTTTGTT+TGG - chr1.2:28470845-28470864 MS.gene53559:CDS 50.0%
! AGCTGCGAAACAGAGCATTC+CGG + chr1.2:28470287-28470306 None:intergenic 50.0%
! CACAGGGTTTGTTTACCCTG+TGG - chr1.2:28470778-28470797 MS.gene53559:CDS 50.0%
! CAGCGCTAACAACGCTTTTC+GGG - chr1.2:28471288-28471307 MS.gene53559:CDS 50.0%
! GCAGCGCTAACAACGCTTTT+CGG - chr1.2:28471287-28471306 MS.gene53559:CDS 50.0%
! GGGTTATTTATGGGTGGTGG+AGG - chr1.2:28471614-28471633 MS.gene53559:CDS 50.0%
! GGTTGGTATGGTTTCAACCC+TGG - chr1.2:28471049-28471068 MS.gene53559:intron 50.0%
! TGACTCCGGTGCCAAACAAA+AGG + chr1.2:28470859-28470878 None:intergenic 50.0%
! TGCTGCCCATGGTTCTACAA+CGG + chr1.2:28471399-28471418 None:intergenic 50.0%
! TGGTTCTACAACGGAACACC+CGG + chr1.2:28471390-28471409 None:intergenic 50.0%
!! GACGTCTGCAACGGATTACT+CGG - chr1.2:28471344-28471363 MS.gene53559:CDS 50.0%
!! GGTGGTATTGCTGGTCTTTG+GGG - chr1.2:28470914-28470933 MS.gene53559:CDS 50.0%
!! TGGCTCCGGTGTTGTTCATA+TGG - chr1.2:28470889-28470908 MS.gene53559:CDS 50.0%
AACGTGACCGACGTCTGCAA+CGG - chr1.2:28471335-28471354 MS.gene53559:CDS 55.0%
AGAGCCGCTGGTAATACCAG+CGG + chr1.2:28470708-28470727 None:intergenic 55.0%
GCTCAATTACACGGTGGGTG+TGG - chr1.2:28471506-28471525 MS.gene53559:CDS 55.0%
GGTAAACTGGGTCGACCCTA+TGG - chr1.2:28471593-28471612 MS.gene53559:CDS 55.0%
GGTGCCAAACAAAAGGTCCC+CGG + chr1.2:28470852-28470871 None:intergenic 55.0%
GGTTTCGCCATGCTTTGTGC+TGG - chr1.2:28470467-28470486 MS.gene53559:CDS 55.0%
TAGGGTCGACCCAGTTTACC+CGG + chr1.2:28471593-28471612 None:intergenic 55.0%
TATGGGCAATGGAGTGCGGT+GGG - chr1.2:28471227-28471246 MS.gene53559:CDS 55.0%
TTATGGGCAATGGAGTGCGG+TGG - chr1.2:28471226-28471245 MS.gene53559:CDS 55.0%
TTCTACAACGGAACACCCGG+CGG + chr1.2:28471387-28471406 None:intergenic 55.0%
! ACTATGTCCGCGAAGTGCCA+CGG + chr1.2:28470990-28471009 None:intergenic 55.0%
! GGTTTTGCTGCGATAACCGC+CGG - chr1.2:28471368-28471387 MS.gene53559:CDS 55.0%
! GTTCTGGTCCGGTGATGGTT+GGG - chr1.2:28470811-28470830 MS.gene53559:CDS 55.0%
! GTTTTGCTGCGATAACCGCC+GGG - chr1.2:28471369-28471388 MS.gene53559:CDS 55.0%
!! CATTGGTTCTGGTCCGGTGA+TGG - chr1.2:28470806-28470825 MS.gene53559:CDS 55.0%
!! GCTTTTGCTATAGCAGCCGC+TGG - chr1.2:28470689-28470708 MS.gene53559:CDS 55.0%
!! GGGGACCTTTTGTTTGGCAC+CGG - chr1.2:28470851-28470870 MS.gene53559:CDS 55.0%
!! GTCTGCAACGGATTACTCGG+CGG - chr1.2:28471347-28471366 MS.gene53559:CDS 55.0%
ATGGGCAATGGAGTGCGGTG+GGG - chr1.2:28471228-28471247 MS.gene53559:CDS 60.0%
GCAGCCGCTGGTATTACCAG+CGG - chr1.2:28470701-28470720 MS.gene53559:CDS 60.0%
GCGGTGACGACGACATTAGC+AGG - chr1.2:28471257-28471276 MS.gene53559:CDS 60.0%
GTCGGTCACGTTCCAGTGAC+CGG + chr1.2:28471327-28471346 None:intergenic 60.0%
TCGCAGCTCGCGCAGTTCAT+CGG - chr1.2:28470299-28470318 MS.gene53559:CDS 60.0%
TCGTTCCGCTATAGAGCCGC+TGG + chr1.2:28470720-28470739 None:intergenic 60.0%
TGCGCTAGCCCAACCATCAC+CGG + chr1.2:28470822-28470841 None:intergenic 60.0%
! GGTTCTGGTCCGGTGATGGT+TGG - chr1.2:28470810-28470829 MS.gene53559:CDS 60.0%
!! TGGGCTAGCGCAACCAACAC+CGG - chr1.2:28470830-28470849 MS.gene53559:CDS 60.0%
AACGTCCTCGACGCTGCTGC+TGG - chr1.2:28470530-28470549 MS.gene53559:CDS 65.0%
ATGGAGTGCGGTGGGGAGGA+CGG - chr1.2:28471235-28471254 MS.gene53559:CDS 65.0%
ATTTGCGGCGGCTTCGGCGT+TGG + chr1.2:28470336-28470355 None:intergenic 65.0%
CGATCACTCTGGTCGAGCCG+TGG - chr1.2:28470970-28470989 MS.gene53559:intron 65.0%
GGCTAGCGCAACCAACACCG+GGG - chr1.2:28470832-28470851 MS.gene53559:CDS 65.0%
! AGCAGCAGCGTCGAGGACGT+TGG + chr1.2:28470531-28470550 None:intergenic 65.0%
! GGGCTAGCGCAACCAACACC+GGG - chr1.2:28470831-28470850 MS.gene53559:CDS 65.0%
!! CTTCGGCGTTGGTGGCGTTG+GGG + chr1.2:28470325-28470344 None:intergenic 65.0%
!! GCTTCGGCGTTGGTGGCGTT+GGG + chr1.2:28470326-28470345 None:intergenic 65.0%
ACACGGTGGGTGTGGCGCGT+GGG - chr1.2:28471514-28471533 MS.gene53559:CDS 70.0%
GGCAATGGAGTGCGGTGGGG+AGG - chr1.2:28471231-28471250 MS.gene53559:CDS 70.0%
GGTCGAGCCGTGGCACTTCG+CGG - chr1.2:28470980-28470999 MS.gene53559:intron 70.0%
GTCCTCGACGCTGCTGCTGG+TGG - chr1.2:28470533-28470552 MS.gene53559:CDS 70.0%
TACACGGTGGGTGTGGCGCG+TGG - chr1.2:28471513-28471532 MS.gene53559:CDS 70.0%
!! GGCTTCGGCGTTGGTGGCGT+TGG + chr1.2:28470327-28470346 None:intergenic 70.0%
CACGGTGGGTGTGGCGCGTG+GGG - chr1.2:28471515-28471534 MS.gene53559:CDS 75.0%
CGCCACCAGCAGCAGCGTCG+AGG + chr1.2:28470538-28470557 None:intergenic 75.0%
GAGTGCGGTGGGGAGGACGG+CGG - chr1.2:28471238-28471257 MS.gene53559:CDS 75.0%
! TGCGGCGGCTTCGGCGTTGG+TGG + chr1.2:28470333-28470352 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 28470275 28471898 28470275 ID=MS.gene53559
chr1.2 mRNA 28470275 28471898 28470275 ID=MS.gene53559.t1;Parent=MS.gene53559
chr1.2 exon 28471067 28471898 28471067 ID=MS.gene53559.t1.exon1;Parent=MS.gene53559.t1
chr1.2 CDS 28471067 28471898 28471067 ID=cds.MS.gene53559.t1;Parent=MS.gene53559.t1
chr1.2 exon 28470275 28470954 28470275 ID=MS.gene53559.t1.exon2;Parent=MS.gene53559.t1
chr1.2 CDS 28470275 28470954 28470275 ID=cds.MS.gene53559.t1;Parent=MS.gene53559.t1
Gene Sequence

>MS.gene53559

ATGTCGCTGCCGGAATGCTCTGTTTCGCAGCTCGCGCAGTTCATCGGCCCCAACGCCACCAACGCCGAAGCCGCCGCAAATTTCATCTGCACTCAGTTCACAGCAGTGTCAAACAGATTCGTCGACACACAATTCGCTGTTGACAACACCTATCTTCTATTTTCAGCTTACCTTGTTTTCTCTATGCAACTTGGTTTCGCCATGCTTTGTGCTGGTTCAGTTCGTGCTAAGAACACCATGAACATCATGCTTACCAACGTCCTCGACGCTGCTGCTGGTGGCGTTTTCTACTATCTCTTCGGTTTTGCCTTTGCCTTTGGAACACCTTCAAATGGTTTCATTGGAAAACATTTCTTCGGTCTGAAGGATGTTCCGACTGAAAATTTTGATTACAGCTATTTTCTCTATCAATGGGCTTTTGCTATAGCAGCCGCTGGTATTACCAGCGGCTCTATAGCGGAACGAACTCAGTTCGTTGCGTATCTTATTTACTCATCGTTTCTCACAGGGTTTGTTTACCCTGTGGTTTCTCATTGGTTCTGGTCCGGTGATGGTTGGGCTAGCGCAACCAACACCGGGGACCTTTTGTTTGGCACCGGAGTCATTGATTTTGCTGGCTCCGGTGTTGTTCATATGGTTGGTGGTATTGCTGGTCTTTGGGGAGCACTTATTGAAGGTCCGCGAATTGGACGGTTCGATCACTCTGGTCGAGCCGTGGCACTTCGCGGACATAGTGCTTCTCTTGTTGTTCTTGGTACCTTCATGCTTTGGTTTGGTTGGTATGGTTTCAACCCTGGTTCCTTCACCAAGATTCTCAGTCCTTATAATTCAGGTAGTTAACTATCTTACTTTTTGTACTAACTACTTTCATTGTTTGGATGCATTAAAATGGAAAACTTTTGTTGAAAAATCATAATTTCTTTTAAATTTTTATGCAATTTTAGGTACTTATTATGGGCAATGGAGTGCGGTGGGGAGGACGGCGGTGACGACGACATTAGCAGGTTGCACGGCAGCGCTAACAACGCTTTTCGGGAAAAGAATTCTCTCCGGTCACTGGAACGTGACCGACGTCTGCAACGGATTACTCGGCGGTTTTGCTGCGATAACCGCCGGGTGTTCCGTTGTAGAACCATGGGCAGCAATAGTATGTGGCTTCATCGCCGCTATTGTTTTAATCGCGTGTAACAAACTCGCGGAGAAGGTGCGTTACGACGATCCATTAGAAGCAGCTCAATTACACGGTGGGTGTGGCGCGTGGGGAATAATATTCACCGCTTTGTTTGCGAAAGAAGAGTATGTGAACCAAGTTTATCCGGGTAAACTGGGTCGACCCTATGGGTTATTTATGGGTGGTGGAGGGAAATTATTAGGGGCACATGTGATACAAATTTTGGTTATTATTGGGTGGGTAAGTGCAACTATGGGTCCACTATTCTTTGTTTTGAATAAATTGAAGTTGTTGAGGATTTCAACTGAAGATGAACTTGCGGGTATGGATTTGACCCGACATGGTGGGTTTGCTTATGCTTATGAAGATGAAGGATCACATAGAAATGGGACTCAATTGAGGAAAATTGATCATAATTCTTCTTCCACTCCTACTCCAACTACAGATTTGTAA
Protein sequence

>MS.gene53559.t1

MSLPECSVSQLAQFIGPNATNAEAAANFICTQFTAVSNRFVDTQFAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGVFYYLFGFAFAFGTPSNGFIGKHFFGLKDVPTENFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSGDGWASATNTGDLLFGTGVIDFAGSGVVHMVGGIAGLWGALIEGPRIGRFDHSGRAVALRGHSASLVVLGTFMLWFGWYGFNPGSFTKILSPYNSGTYYGQWSAVGRTAVTTTLAGCTAALTTLFGKRILSGHWNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFIAAIVLIACNKLAEKVRYDDPLEAAQLHGGCGAWGIIFTALFAKEEYVNQVYPGKLGRPYGLFMGGGGKLLGAHVIQILVIIGWVSATMGPLFFVLNKLKLLRISTEDELAGMDLTRHGGFAYAYEDEGSHRNGTQLRKIDHNSSSTPTPTTDL