AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53734

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53734.t1 MTR_1g048320 91.279 344 25 2 1 339 1 344 0.0 613
MS.gene53734.t1 MTR_1g031060 71.651 321 83 5 24 339 45 362 1.29e-148 423
MS.gene53734.t1 MTR_1g031080 60.870 345 72 6 2 339 4 292 5.25e-134 383
MS.gene53734.t1 MTR_2g087610 56.757 296 116 4 56 339 73 368 1.56e-108 321
MS.gene53734.t1 MTR_3g106930 48.475 295 124 6 61 339 95 377 3.60e-80 249
MS.gene53734.t1 MTR_2g005350 39.024 287 147 9 60 336 168 436 2.42e-51 176
MS.gene53734.t1 MTR_1g110290 35.806 310 174 6 35 336 99 391 3.80e-50 172
MS.gene53734.t1 MTR_2g037600 34.098 305 149 8 58 331 103 386 1.68e-48 167
MS.gene53734.t1 MTR_4g127550 34.539 304 146 8 59 331 106 387 2.19e-48 167
MS.gene53734.t1 MTR_5g055480 38.328 287 147 8 60 336 122 388 6.79e-48 166
MS.gene53734.t1 MTR_8g015500 35.315 286 159 7 60 336 143 411 1.23e-47 166
MS.gene53734.t1 MTR_1g009960 34.098 305 155 6 53 329 104 390 1.23e-45 160
MS.gene53734.t1 MTR_2g037620 33.993 303 147 8 60 331 97 377 1.54e-45 159
MS.gene53734.t1 MTR_3g108740 36.156 307 142 9 55 329 87 371 1.57e-45 159
MS.gene53734.t1 MTR_1g009960 35.338 266 149 5 53 313 104 351 1.25e-44 157
MS.gene53734.t1 MTR_5g006930 36.620 284 150 9 60 332 116 380 1.33e-44 157
MS.gene53734.t1 MTR_1g016880 34.520 281 157 8 60 331 106 368 7.54e-44 155
MS.gene53734.t1 MTR_6g083830 35.689 283 154 7 60 332 149 413 8.93e-44 155
MS.gene53734.t1 MTR_5g006920 36.268 284 151 9 60 332 151 415 5.54e-42 151
MS.gene53734.t1 MTR_3g104840 34.202 307 158 7 35 332 116 387 1.13e-41 149
MS.gene53734.t1 MTR_2g024120 29.167 336 165 13 39 321 36 351 1.71e-27 111
MS.gene53734.t1 MTR_8g094180 27.217 327 167 9 59 332 54 362 3.49e-25 104
MS.gene53734.t1 MTR_8g094180 29.091 275 147 8 59 303 54 310 3.66e-25 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53734.t1 AT3G52060 57.325 314 115 6 35 339 43 346 1.40e-122 356
MS.gene53734.t1 AT5G22070 51.253 359 144 9 9 339 7 362 1.29e-115 339
MS.gene53734.t1 AT5G25330 44.648 327 142 10 33 339 55 362 6.60e-79 246
MS.gene53734.t1 AT4G32290 46.130 323 140 7 37 339 71 379 3.84e-77 241
MS.gene53734.t1 AT4G31350 38.538 301 135 8 56 329 84 361 1.44e-58 193
MS.gene53734.t1 AT4G31350 38.538 301 140 8 56 329 84 366 1.61e-57 191
MS.gene53734.t1 AT4G31350 38.538 301 140 8 56 329 84 366 1.61e-57 191
MS.gene53734.t1 AT4G31350 38.538 301 140 8 56 329 84 366 1.61e-57 191
MS.gene53734.t1 AT5G25970 38.596 285 148 9 57 332 115 381 1.40e-51 176
MS.gene53734.t1 AT5G25970 38.596 285 148 9 57 332 115 381 1.40e-51 176
MS.gene53734.t1 AT5G25970 38.596 285 148 9 57 332 115 381 1.40e-51 176
MS.gene53734.t1 AT3G21310 36.713 286 154 7 60 336 114 381 3.91e-50 172
MS.gene53734.t1 AT3G21310 36.713 286 154 7 60 336 114 381 3.91e-50 172
MS.gene53734.t1 AT1G51770 35.664 286 157 7 60 336 123 390 1.18e-47 166
MS.gene53734.t1 AT5G11730 34.965 286 159 7 60 336 117 384 2.56e-47 164
MS.gene53734.t1 AT1G10280 35.192 287 158 8 60 336 142 410 6.37e-46 161
MS.gene53734.t1 AT1G10880 34.520 281 159 6 60 332 117 380 2.91e-45 159
MS.gene53734.t1 AT4G25870 34.835 333 156 11 33 331 75 380 3.60e-45 159
MS.gene53734.t1 AT1G68390 33.866 313 178 7 35 336 112 406 3.10e-44 157
MS.gene53734.t1 AT1G68380 34.877 324 174 9 28 337 91 391 1.05e-43 155
MS.gene53734.t1 AT2G19160 34.323 303 145 9 61 331 106 386 3.36e-43 154
MS.gene53734.t1 AT2G19160 34.323 303 145 9 61 331 106 386 3.36e-43 154
MS.gene53734.t1 AT4G30060 34.323 303 145 8 61 331 109 389 7.24e-43 153
MS.gene53734.t1 AT4G30060 34.323 303 145 8 61 331 109 389 7.24e-43 153
MS.gene53734.t1 AT5G57270 35.252 278 149 6 59 327 96 351 1.70e-42 152
MS.gene53734.t1 AT5G57270 35.252 278 149 6 59 327 96 351 1.70e-42 152
MS.gene53734.t1 AT5G57270 35.252 278 149 6 59 327 96 351 1.70e-42 152
MS.gene53734.t1 AT5G57270 35.252 278 149 6 59 327 96 351 1.70e-42 152
MS.gene53734.t1 AT5G57270 35.252 278 149 6 59 327 96 351 1.70e-42 152
MS.gene53734.t1 AT5G16170 34.495 287 154 8 61 332 136 403 3.41e-40 146
MS.gene53734.t1 AT1G73810 36.644 292 147 11 61 336 147 416 1.90e-39 144
MS.gene53734.t1 AT1G51770 31.469 286 142 7 60 336 123 363 4.83e-35 131
MS.gene53734.t1 AT5G14550 29.392 296 170 8 44 318 44 321 1.35e-30 119
MS.gene53734.t1 AT5G14550 27.988 343 176 10 44 333 44 368 5.85e-29 115
MS.gene53734.t1 AT5G14550 27.900 319 177 9 44 327 44 344 1.08e-28 114
MS.gene53734.t1 AT1G11940 28.528 326 169 9 45 324 58 365 1.70e-28 114
MS.gene53734.t1 AT1G62305 29.003 331 154 14 45 321 53 356 1.17e-26 108
MS.gene53734.t1 AT1G62305 31.200 250 119 10 45 267 53 276 2.01e-23 99.0
MS.gene53734.t1 AT5G14550 27.356 329 169 12 44 333 44 341 1.83e-21 94.0
MS.gene53734.t1 AT1G11940 33.173 208 115 8 45 247 58 246 2.60e-20 89.4
MS.gene53734.t1 AT3G21310 45.133 113 59 3 60 171 114 224 4.65e-20 88.6

Find 109 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCACCTTTGCTCCTTTA+TGG 0.074069 1.3:+30186573 MS.gene53734:CDS
TTGAATCTTGTACTTGTTTA+AGG 0.108034 1.3:-30186882 None:intergenic
TAGGAAGAGGAAGGCGATTT+TGG 0.185523 1.3:-30186540 None:intergenic
GTTGAAGAGGTGGTTGTTTC+CGG 0.217030 1.3:-30186609 None:intergenic
GTTGGAAAGTAATGCTCTTC+TGG 0.233444 1.3:-30187124 None:intergenic
CCTCACCTTTGCTCCTTTAT+GGG 0.265226 1.3:+30186574 MS.gene53734:CDS
CCTTTGCATGGTTCCGGTTC+AGG 0.283118 1.3:-30187033 None:intergenic
AGGTCCTCCGCGTCGGGGTC+AGG 0.290705 1.3:-30186455 None:intergenic
AAGGGATGAAGTGGCTTTGA+TGG 0.297513 1.3:-30186685 None:intergenic
AAAAGTTGTGGAAGAAGTTT+CGG 0.336219 1.3:+30187071 MS.gene53734:CDS
TTACTCTTACGAGAGTTAAC+TGG 0.341127 1.3:+30187185 MS.gene53734:CDS
AGGTGAGGTTGGTATTTGTT+AGG 0.341432 1.3:-30186559 None:intergenic
GAACAACCTTTGCATGGTTC+CGG 0.347319 1.3:-30187039 None:intergenic
GTAATGCTCTTCTGGGTAAC+AGG 0.352431 1.3:-30187116 None:intergenic
TTATGGGAAATATTCTTTGC+CGG 0.354403 1.3:+30186590 MS.gene53734:CDS
ATCAACGGTGCTAGTGTTTC+CGG 0.362374 1.3:-30187325 None:intergenic
CGCAGTTCTTTATCCTGAAC+CGG 0.364355 1.3:+30187020 MS.gene53734:CDS
CGGGGTCAGGGTTGGAAAAC+GGG 0.368387 1.3:-30186442 None:intergenic
TCTCTTAGCCACTGCTATAT+TGG 0.370952 1.3:+30186757 MS.gene53734:CDS
GGGTGAGACAACAGAGGAAA+TGG 0.381450 1.3:-30186651 None:intergenic
AGAGCAAGGTCCTCCGCGTC+GGG 0.395834 1.3:-30186461 None:intergenic
TGTACGCCAGCTTAGGAAAT+CGG 0.400199 1.3:+30187264 MS.gene53734:CDS
GCAAAGAATATTTCCCATAA+AGG 0.406338 1.3:-30186587 None:intergenic
GTTCTTTAGGTTAAATAAGT+GGG 0.421988 1.3:-30186510 None:intergenic
TCGGGGTCAGGGTTGGAAAA+CGG 0.425351 1.3:-30186443 None:intergenic
GGAAACACTAGCACCGTTGA+TGG 0.436751 1.3:+30187327 MS.gene53734:CDS
CGGAGCTTGTACGCCAGCTT+AGG 0.440581 1.3:+30187257 MS.gene53734:CDS
GGTCCTCCGCGTCGGGGTCA+GGG 0.440712 1.3:-30186454 None:intergenic
GCTTTGATGGAAGACGCCTC+CGG 0.443380 1.3:-30186672 None:intergenic
GGTTGGTATTTGTTAGGAAG+AGG 0.449439 1.3:-30186553 None:intergenic
GTAAAACCAGTGCAGCCATC+CGG 0.456279 1.3:-30187166 None:intergenic
TCTTCTATCGATGGAGGACC+CGG 0.458996 1.3:+30187147 MS.gene53734:CDS
CTCTCTTCCGCCGCGCCACA+AGG 0.462891 1.3:+30186480 MS.gene53734:CDS
CTCCGCGTCGGGGTCAGGGT+TGG 0.467120 1.3:-30186450 None:intergenic
AATACAATGTTGGGAGAGTA+AGG 0.471648 1.3:-30186804 None:intergenic
TTGGAAAGTAATGCTCTTCT+GGG 0.472293 1.3:-30187123 None:intergenic
GATGCCGGAGGTTTCATTCA+AGG 0.472977 1.3:+30186982 MS.gene53734:CDS
GGTTCTTTAGGTTAAATAAG+TGG 0.477319 1.3:-30186511 None:intergenic
ATTTCCTCTGTTGTCTCACC+CGG 0.480740 1.3:+30186653 MS.gene53734:CDS
AAGTGACGGAGAGGCACGTT+GGG 0.481033 1.3:-30186717 None:intergenic
AAGTCAATCTCAATGAAGCT+AGG 0.484069 1.3:-30186908 None:intergenic
GAGGACCCGGATGGCTGCAC+TGG 0.486529 1.3:+30187160 MS.gene53734:CDS
GAAGAGTGGAATACAATGTT+GGG 0.491853 1.3:-30186813 None:intergenic
GAATTTCCTTGCAAACAGAT+AGG 0.494280 1.3:-30187299 None:intergenic
CTTTCCAACTCTTCTATCGA+TGG 0.497559 1.3:+30187138 MS.gene53734:CDS
CTAAGCTGGCGTACAAGCTC+CGG 0.504844 1.3:-30187256 None:intergenic
CTTTGATGGAAGACGCCTCC+GGG 0.508919 1.3:-30186671 None:intergenic
CAGGAACTCTTATCTATACA+TGG 0.509702 1.3:-30187097 None:intergenic
TAAAATCCGATTTCCTAAGC+TGG 0.511119 1.3:-30187270 None:intergenic
GTTATTCCTATCTGTTTGCA+AGG 0.511432 1.3:+30187293 MS.gene53734:CDS
TTATAGCGCTGGTAGAGATT+CGG 0.513272 1.3:-30186938 None:intergenic
TTCCACGAGCATTATAGCGC+TGG 0.514680 1.3:-30186949 None:intergenic
TACTCGAGAGGAGGTGTCGC+CGG 0.514897 1.3:+30187237 MS.gene53734:CDS
AGAAGAGTGGAATACAATGT+TGG 0.514911 1.3:-30186814 None:intergenic
TGCAGTACAAGTTTCAATCT+CGG 0.523348 1.3:-30187372 None:intergenic
AGTTAACTGGACTGGTTGTA+CGG 0.524550 1.3:+30187198 MS.gene53734:CDS
TCCTCCATCGATAGAAGAGT+TGG 0.526190 1.3:-30187142 None:intergenic
GGGAGAGTAAGGCAAAGTAT+TGG 0.537563 1.3:-30186793 None:intergenic
AAATCCTTGAATGAAACCTC+CGG 0.545006 1.3:-30186986 None:intergenic
GGTGAGACAACAGAGGAAAT+GGG 0.547850 1.3:-30186650 None:intergenic
CTTACGAGAGTTAACTGGAC+TGG 0.548736 1.3:+30187190 MS.gene53734:CDS
GGTATTTGTTAGGAAGAGGA+AGG 0.549687 1.3:-30186549 None:intergenic
TTCTTTAGGTTAAATAAGTG+GGG 0.550325 1.3:-30186509 None:intergenic
TAAAACCAGTGCAGCCATCC+GGG 0.551737 1.3:-30187165 None:intergenic
AGTCAATCTCAATGAAGCTA+GGG 0.551844 1.3:-30186907 None:intergenic
GGCTAAGAGACGGCGAGCAG+CGG 0.557898 1.3:-30186744 None:intergenic
GCGCTATAATGCTCGTGGAA+AGG 0.565767 1.3:+30186952 MS.gene53734:CDS
AGGCAAAGTATTGGTTATGC+GGG 0.566058 1.3:-30186784 None:intergenic
TGGAAAGGATGTAATGATGC+CGG 0.568418 1.3:+30186967 MS.gene53734:CDS
GAAAACGGGAAGTAAGATTG+AGG 0.570111 1.3:-30186428 None:intergenic
TAAAGGAGCAAAGGTGAGGT+TGG 0.572056 1.3:-30186570 None:intergenic
TTCTGCGAGATCAAAAGTTG+TGG 0.575067 1.3:+30187059 MS.gene53734:CDS
GAGAGCAAGGTCCTCCGCGT+CGG 0.575874 1.3:-30186462 None:intergenic
AAAACGGGAAGTAAGATTGA+GGG 0.576076 1.3:-30186427 None:intergenic
TCCTCTCGAGTATAAAGATG+CGG 0.582936 1.3:-30187226 None:intergenic
TCCTCTGTTGTCTCACCCGG+AGG 0.583294 1.3:+30186656 MS.gene53734:CDS
TACCAGCGCTATAATGCTCG+TGG 0.583876 1.3:+30186947 MS.gene53734:CDS
CTCGCAGAACAACCTTTGCA+TGG 0.585317 1.3:-30187045 None:intergenic
GTTTGCAAGGAAATTCTCAC+CGG 0.585980 1.3:+30187306 MS.gene53734:CDS
CCCATAAAGGAGCAAAGGTG+AGG 0.594602 1.3:-30186574 None:intergenic
TGGCGCGGCGGAAGAGAGCA+AGG 0.594820 1.3:-30186475 None:intergenic
CCTCTCGAGTATAAAGATGC+GGG 0.597296 1.3:-30187225 None:intergenic
AAGGCAAAGTATTGGTTATG+CGG 0.608135 1.3:-30186785 None:intergenic
TATTTCCCATAAAGGAGCAA+AGG 0.610784 1.3:-30186579 None:intergenic
ACAAATCGAAACGAGAAGAG+TGG 0.612100 1.3:-30186827 None:intergenic
CGAGCAGCGGCTATAAGTGA+CGG 0.620401 1.3:-30186731 None:intergenic
CTATCGATGGAGGACCCGGA+TGG 0.620435 1.3:+30187151 MS.gene53734:CDS
AAACGGGAAGTAAGATTGAG+GGG 0.627961 1.3:-30186426 None:intergenic
ATATAGCAGTGGCTAAGAGA+CGG 0.629423 1.3:-30186754 None:intergenic
TCCAACTCTTCTATCGATGG+AGG 0.630039 1.3:+30187141 MS.gene53734:CDS
CAACCCTGACCCCGACGCGG+AGG 0.630344 1.3:+30186451 MS.gene53734:CDS
GGATGTAGATGTTGAAGAGG+TGG 0.641305 1.3:-30186619 None:intergenic
TTCCAACCCTGACCCCGACG+CGG 0.643455 1.3:+30186448 MS.gene53734:CDS
TCATAAGCTATATCCATCAA+CGG 0.653143 1.3:-30187340 None:intergenic
CGGGTCATCCAATATAGCAG+TGG 0.653653 1.3:-30186765 None:intergenic
CGTGGATGTAGATGTTGAAG+AGG 0.655044 1.3:-30186622 None:intergenic
GGGGTGTTGTCCTTGTGGCG+CGG 0.655134 1.3:-30186490 None:intergenic
GAGCAAGGTCCTCCGCGTCG+GGG 0.655727 1.3:-30186460 None:intergenic
TAAGTGACGGAGAGGCACGT+TGG 0.660309 1.3:-30186718 None:intergenic
CCTGAACCGGAACCATGCAA+AGG 0.664001 1.3:+30187033 MS.gene53734:CDS
AGCGGCTATAAGTGACGGAG+AGG 0.667766 1.3:-30186726 None:intergenic
CCCGCATCTTTATACTCGAG+AGG 0.669713 1.3:+30187225 MS.gene53734:CDS
CTCGAGTATAAAGATGCGGG+TGG 0.683495 1.3:-30187222 None:intergenic
TAAGTGGGGTGTTGTCCTTG+TGG 0.684395 1.3:-30186495 None:intergenic
GCATCTTTATACTCGAGAGG+AGG 0.684710 1.3:+30187228 MS.gene53734:CDS
GTGTTGTCCTTGTGGCGCGG+CGG 0.702688 1.3:-30186487 None:intergenic
CAGAGGAAATGGGATCTGCG+TGG 0.703706 1.3:-30186640 None:intergenic
AAAGGATGTAATGATGCCGG+AGG 0.717780 1.3:+30186970 MS.gene53734:CDS
GCCTCCGGGTGAGACAACAG+AGG 0.763777 1.3:-30186657 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATCCCTTTTTGAAAAT+CGG + chr1.3:30186852-30186871 MS.gene53734:CDS 20.0%
! TTGAATCTTGTACTTGTTTA+AGG - chr1.3:30186885-30186904 None:intergenic 25.0%
!! GTTCTTTAGGTTAAATAAGT+GGG - chr1.3:30186513-30186532 None:intergenic 25.0%
!! TTCTTTAGGTTAAATAAGTG+GGG - chr1.3:30186512-30186531 None:intergenic 25.0%
!!! TAAATCCCTTTTTGAAAATC+GGG + chr1.3:30186853-30186872 MS.gene53734:CDS 25.0%
AAAAGTTGTGGAAGAAGTTT+CGG + chr1.3:30187071-30187090 MS.gene53734:CDS 30.0%
GCAAAGAATATTTCCCATAA+AGG - chr1.3:30186590-30186609 None:intergenic 30.0%
TCATAAGCTATATCCATCAA+CGG - chr1.3:30187343-30187362 None:intergenic 30.0%
TTATGGGAAATATTCTTTGC+CGG + chr1.3:30186590-30186609 MS.gene53734:CDS 30.0%
! GGTTCTTTAGGTTAAATAAG+TGG - chr1.3:30186514-30186533 None:intergenic 30.0%
AAAACGGGAAGTAAGATTGA+GGG - chr1.3:30186430-30186449 None:intergenic 35.0%
AAATCCTTGAATGAAACCTC+CGG - chr1.3:30186989-30187008 None:intergenic 35.0%
AAGTCAATCTCAATGAAGCT+AGG - chr1.3:30186911-30186930 None:intergenic 35.0%
AATACAATGTTGGGAGAGTA+AGG - chr1.3:30186807-30186826 None:intergenic 35.0%
AGAAGAGTGGAATACAATGT+TGG - chr1.3:30186817-30186836 None:intergenic 35.0%
AGTCAATCTCAATGAAGCTA+GGG - chr1.3:30186910-30186929 None:intergenic 35.0%
CAGGAACTCTTATCTATACA+TGG - chr1.3:30187100-30187119 None:intergenic 35.0%
GAAGAGTGGAATACAATGTT+GGG - chr1.3:30186816-30186835 None:intergenic 35.0%
GAATTTCCTTGCAAACAGAT+AGG - chr1.3:30187302-30187321 None:intergenic 35.0%
GTTATTCCTATCTGTTTGCA+AGG + chr1.3:30187293-30187312 MS.gene53734:CDS 35.0%
TAAAATCCGATTTCCTAAGC+TGG - chr1.3:30187273-30187292 None:intergenic 35.0%
TATTTCCCATAAAGGAGCAA+AGG - chr1.3:30186582-30186601 None:intergenic 35.0%
TTACTCTTACGAGAGTTAAC+TGG + chr1.3:30187185-30187204 MS.gene53734:CDS 35.0%
TTGGAAAGTAATGCTCTTCT+GGG - chr1.3:30187126-30187145 None:intergenic 35.0%
! ACTCTACCCGATTTTCAAAA+AGG - chr1.3:30186862-30186881 None:intergenic 35.0%
! CTCTACCCGATTTTCAAAAA+GGG - chr1.3:30186861-30186880 None:intergenic 35.0%
! TCATTCAAGGATTTTCGCAA+AGG + chr1.3:30186995-30187014 MS.gene53734:CDS 35.0%
!! AAGGCAAAGTATTGGTTATG+CGG - chr1.3:30186788-30186807 None:intergenic 35.0%
!!! TTGTTTTAGAAGGGATGAAG+TGG - chr1.3:30186697-30186716 None:intergenic 35.0%
!!! TTTTGGGTTTAGGGTTCTTT+AGG - chr1.3:30186526-30186545 None:intergenic 35.0%
AAACGGGAAGTAAGATTGAG+GGG - chr1.3:30186429-30186448 None:intergenic 40.0%
ACAAATCGAAACGAGAAGAG+TGG - chr1.3:30186830-30186849 None:intergenic 40.0%
ATATAGCAGTGGCTAAGAGA+CGG - chr1.3:30186757-30186776 None:intergenic 40.0%
CTTTCCAACTCTTCTATCGA+TGG + chr1.3:30187138-30187157 MS.gene53734:CDS 40.0%
GAAAACGGGAAGTAAGATTG+AGG - chr1.3:30186431-30186450 None:intergenic 40.0%
GTTGGAAAGTAATGCTCTTC+TGG - chr1.3:30187127-30187146 None:intergenic 40.0%
GTTTGCAAGGAAATTCTCAC+CGG + chr1.3:30187306-30187325 MS.gene53734:CDS 40.0%
TCCTCTCGAGTATAAAGATG+CGG - chr1.3:30187229-30187248 None:intergenic 40.0%
TCTCTTAGCCACTGCTATAT+TGG + chr1.3:30186757-30186776 MS.gene53734:CDS 40.0%
TGGAAAGGATGTAATGATGC+CGG + chr1.3:30186967-30186986 MS.gene53734:CDS 40.0%
TTCTGCGAGATCAAAAGTTG+TGG + chr1.3:30187059-30187078 MS.gene53734:CDS 40.0%
! GGTATTTGTTAGGAAGAGGA+AGG - chr1.3:30186552-30186571 None:intergenic 40.0%
! GGTTGGTATTTGTTAGGAAG+AGG - chr1.3:30186556-30186575 None:intergenic 40.0%
!! AGGCAAAGTATTGGTTATGC+GGG - chr1.3:30186787-30186806 None:intergenic 40.0%
!! AGGTGAGGTTGGTATTTGTT+AGG - chr1.3:30186562-30186581 None:intergenic 40.0%
!! AGTTAACTGGACTGGTTGTA+CGG + chr1.3:30187198-30187217 MS.gene53734:CDS 40.0%
!! TTATAGCGCTGGTAGAGATT+CGG - chr1.3:30186941-30186960 None:intergenic 40.0%
!!! CACGTTGGGTTGTTTTAGAA+GGG - chr1.3:30186706-30186725 None:intergenic 40.0%
AAAGGATGTAATGATGCCGG+AGG + chr1.3:30186970-30186989 MS.gene53734:CDS 45.0%
ACCTCACCTTTGCTCCTTTA+TGG + chr1.3:30186573-30186592 MS.gene53734:CDS 45.0%
ATTTCCTCTGTTGTCTCACC+CGG + chr1.3:30186653-30186672 MS.gene53734:CDS 45.0%
CCTCACCTTTGCTCCTTTAT+GGG + chr1.3:30186574-30186593 MS.gene53734:CDS 45.0%
CCTCTCGAGTATAAAGATGC+GGG - chr1.3:30187228-30187247 None:intergenic 45.0%
CGCAGTTCTTTATCCTGAAC+CGG + chr1.3:30187020-30187039 MS.gene53734:CDS 45.0%
CGTGGATGTAGATGTTGAAG+AGG - chr1.3:30186625-30186644 None:intergenic 45.0%
CTTACGAGAGTTAACTGGAC+TGG + chr1.3:30187190-30187209 MS.gene53734:CDS 45.0%
GAACAACCTTTGCATGGTTC+CGG - chr1.3:30187042-30187061 None:intergenic 45.0%
GCATCTTTATACTCGAGAGG+AGG + chr1.3:30187228-30187247 MS.gene53734:CDS 45.0%
GGATGTAGATGTTGAAGAGG+TGG - chr1.3:30186622-30186641 None:intergenic 45.0%
GGGAGAGTAAGGCAAAGTAT+TGG - chr1.3:30186796-30186815 None:intergenic 45.0%
GGTGAGACAACAGAGGAAAT+GGG - chr1.3:30186653-30186672 None:intergenic 45.0%
GTAATGCTCTTCTGGGTAAC+AGG - chr1.3:30187119-30187138 None:intergenic 45.0%
TAAAGGAGCAAAGGTGAGGT+TGG - chr1.3:30186573-30186592 None:intergenic 45.0%
TCCAACTCTTCTATCGATGG+AGG + chr1.3:30187141-30187160 MS.gene53734:CDS 45.0%
TCCTCCATCGATAGAAGAGT+TGG - chr1.3:30187145-30187164 None:intergenic 45.0%
TGTACGCCAGCTTAGGAAAT+CGG + chr1.3:30187264-30187283 MS.gene53734:CDS 45.0%
! AGGAAGAGGAAGGCGATTTT+GGG - chr1.3:30186542-30186561 None:intergenic 45.0%
! ATCAACGGTGCTAGTGTTTC+CGG - chr1.3:30187328-30187347 None:intergenic 45.0%
! GTTGAAGAGGTGGTTGTTTC+CGG - chr1.3:30186612-30186631 None:intergenic 45.0%
! TAGGAAGAGGAAGGCGATTT+TGG - chr1.3:30186543-30186562 None:intergenic 45.0%
!! AAGGGATGAAGTGGCTTTGA+TGG - chr1.3:30186688-30186707 None:intergenic 45.0%
!!! AGGAAGGCGATTTTGGGTTT+AGG - chr1.3:30186536-30186555 None:intergenic 45.0%
!!! GCACGTTGGGTTGTTTTAGA+AGG - chr1.3:30186707-30186726 None:intergenic 45.0%
!!! GGAAGGCGATTTTGGGTTTA+GGG - chr1.3:30186535-30186554 None:intergenic 45.0%
CCCATAAAGGAGCAAAGGTG+AGG - chr1.3:30186577-30186596 None:intergenic 50.0%
CCCGCATCTTTATACTCGAG+AGG + chr1.3:30187225-30187244 MS.gene53734:CDS 50.0%
CGGGTCATCCAATATAGCAG+TGG - chr1.3:30186768-30186787 None:intergenic 50.0%
CTCGAGTATAAAGATGCGGG+TGG - chr1.3:30187225-30187244 None:intergenic 50.0%
CTCGCAGAACAACCTTTGCA+TGG - chr1.3:30187048-30187067 None:intergenic 50.0%
GATGCCGGAGGTTTCATTCA+AGG + chr1.3:30186982-30187001 MS.gene53734:CDS 50.0%
GCGCTATAATGCTCGTGGAA+AGG + chr1.3:30186952-30186971 MS.gene53734:CDS 50.0%
GGAAACACTAGCACCGTTGA+TGG + chr1.3:30187327-30187346 MS.gene53734:CDS 50.0%
GGGTGAGACAACAGAGGAAA+TGG - chr1.3:30186654-30186673 None:intergenic 50.0%
GTAAAACCAGTGCAGCCATC+CGG - chr1.3:30187169-30187188 None:intergenic 50.0%
TAAAACCAGTGCAGCCATCC+GGG - chr1.3:30187168-30187187 None:intergenic 50.0%
TACCAGCGCTATAATGCTCG+TGG + chr1.3:30186947-30186966 MS.gene53734:CDS 50.0%
TCTTCTATCGATGGAGGACC+CGG + chr1.3:30187147-30187166 MS.gene53734:CDS 50.0%
TTCCACGAGCATTATAGCGC+TGG - chr1.3:30186952-30186971 None:intergenic 50.0%
!! TAAGTGGGGTGTTGTCCTTG+TGG - chr1.3:30186498-30186517 None:intergenic 50.0%
CAGAGGAAATGGGATCTGCG+TGG - chr1.3:30186643-30186662 None:intergenic 55.0%
CCTGAACCGGAACCATGCAA+AGG + chr1.3:30187033-30187052 MS.gene53734:CDS 55.0%
CCTTTGCATGGTTCCGGTTC+AGG - chr1.3:30187036-30187055 None:intergenic 55.0%
CGAGCAGCGGCTATAAGTGA+CGG - chr1.3:30186734-30186753 None:intergenic 55.0%
CTAAGCTGGCGTACAAGCTC+CGG - chr1.3:30187259-30187278 None:intergenic 55.0%
CTTTGATGGAAGACGCCTCC+GGG - chr1.3:30186674-30186693 None:intergenic 55.0%
GCTTTGATGGAAGACGCCTC+CGG - chr1.3:30186675-30186694 None:intergenic 55.0%
TCGGGGTCAGGGTTGGAAAA+CGG - chr1.3:30186446-30186465 None:intergenic 55.0%
! AAGTGACGGAGAGGCACGTT+GGG - chr1.3:30186720-30186739 None:intergenic 55.0%
! AGCGGCTATAAGTGACGGAG+AGG - chr1.3:30186729-30186748 None:intergenic 55.0%
! TAAGTGACGGAGAGGCACGT+TGG - chr1.3:30186721-30186740 None:intergenic 55.0%
CGGAGCTTGTACGCCAGCTT+AGG + chr1.3:30187257-30187276 MS.gene53734:CDS 60.0%
CGGGGTCAGGGTTGGAAAAC+GGG - chr1.3:30186445-30186464 None:intergenic 60.0%
CTATCGATGGAGGACCCGGA+TGG + chr1.3:30187151-30187170 MS.gene53734:CDS 60.0%
TCCTCTGTTGTCTCACCCGG+AGG + chr1.3:30186656-30186675 MS.gene53734:CDS 60.0%
!! TACTCGAGAGGAGGTGTCGC+CGG + chr1.3:30187237-30187256 MS.gene53734:CDS 60.0%
AGAGCAAGGTCCTCCGCGTC+GGG - chr1.3:30186464-30186483 None:intergenic 65.0%
GAGAGCAAGGTCCTCCGCGT+CGG - chr1.3:30186465-30186484 None:intergenic 65.0%
GCCTCCGGGTGAGACAACAG+AGG - chr1.3:30186660-30186679 None:intergenic 65.0%
GGCTAAGAGACGGCGAGCAG+CGG - chr1.3:30186747-30186766 None:intergenic 65.0%
GTGTTGTCCTTGTGGCGCGG+CGG - chr1.3:30186490-30186509 None:intergenic 65.0%
TTCCAACCCTGACCCCGACG+CGG + chr1.3:30186448-30186467 MS.gene53734:CDS 65.0%
!! GGGGTGTTGTCCTTGTGGCG+CGG - chr1.3:30186493-30186512 None:intergenic 65.0%
CTCTCTTCCGCCGCGCCACA+AGG + chr1.3:30186480-30186499 MS.gene53734:CDS 70.0%
GAGCAAGGTCCTCCGCGTCG+GGG - chr1.3:30186463-30186482 None:intergenic 70.0%
GAGGACCCGGATGGCTGCAC+TGG + chr1.3:30187160-30187179 MS.gene53734:CDS 70.0%
TGGCGCGGCGGAAGAGAGCA+AGG - chr1.3:30186478-30186497 None:intergenic 70.0%
AGGTCCTCCGCGTCGGGGTC+AGG - chr1.3:30186458-30186477 None:intergenic 75.0%
CAACCCTGACCCCGACGCGG+AGG + chr1.3:30186451-30186470 MS.gene53734:CDS 75.0%
CTCCGCGTCGGGGTCAGGGT+TGG - chr1.3:30186453-30186472 None:intergenic 75.0%
GGTCCTCCGCGTCGGGGTCA+GGG - chr1.3:30186457-30186476 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 30186362 30187381 30186362 ID=MS.gene53734
chr1.3 mRNA 30186362 30187381 30186362 ID=MS.gene53734.t1;Parent=MS.gene53734
chr1.3 exon 30186362 30187381 30186362 ID=MS.gene53734.t1.exon1;Parent=MS.gene53734.t1
chr1.3 CDS 30186362 30187381 30186362 ID=cds.MS.gene53734.t1;Parent=MS.gene53734.t1
Gene Sequence

>MS.gene53734

ATGTCGAAATTACGCGTTCTGATAGTCTTGTTATTTTCTCTCTTAATCACTCTCTTCTTTTTCTCCCCTCAATCTTACTTCCCGTTTTCCAACCCTGACCCCGACGCGGAGGACCTTGCTCTCTTCCGCCGCGCCACAAGGACAACACCCCACTTATTTAACCTAAAGAACCCTAAACCCAAAATCGCCTTCCTCTTCCTAACAAATACCAACCTCACCTTTGCTCCTTTATGGGAAATATTCTTTGCCGGAAACAACCACCTCTTCAACATCTACATCCACGCAGATCCCATTTCCTCTGTTGTCTCACCCGGAGGCGTCTTCCATCAAAGCCACTTCATCCCTTCTAAAACAACCCAACGTGCCTCTCCGTCACTTATAGCCGCTGCTCGCCGTCTCTTAGCCACTGCTATATTGGATGACCCGCATAACCAATACTTTGCCTTACTCTCCCAACATTGTATTCCACTCTTCTCGTTTCGATTTGTTTATAAATCCCTTTTTGAAAATCGGGTAGAGTCCTTAAACAAGTACAAGATTCAACACCCTAGCTTCATTGAGATTGACTTTAAACATCCGAATCTCTACCAGCGCTATAATGCTCGTGGAAAGGATGTAATGATGCCGGAGGTTTCATTCAAGGATTTTCGCAAAGGCTCGCAGTTCTTTATCCTGAACCGGAACCATGCAAAGGTTGTTCTGCGAGATCAAAAGTTGTGGAAGAAGTTTCGGATTCCATGTATAGATAAGAGTTCCTGTTACCCAGAAGAGCATTACTTTCCAACTCTTCTATCGATGGAGGACCCGGATGGCTGCACTGGTTTTACTCTTACGAGAGTTAACTGGACTGGTTGTACGGACCACCCGCATCTTTATACTCGAGAGGAGGTGTCGCCGGAGCTTGTACGCCAGCTTAGGAAATCGGATTTTAGTTATTCCTATCTGTTTGCAAGGAAATTCTCACCGGAAACACTAGCACCGTTGATGGATATAGCTTATGATGTCATTTTCCGAGATTGA
Protein sequence

>MS.gene53734.t1

MSKLRVLIVLLFSLLITLFFFSPQSYFPFSNPDPDAEDLALFRRATRTTPHLFNLKNPKPKIAFLFLTNTNLTFAPLWEIFFAGNNHLFNIYIHADPISSVVSPGGVFHQSHFIPSKTTQRASPSLIAAARRLLATAILDDPHNQYFALLSQHCIPLFSFRFVYKSLFENRVESLNKYKIQHPSFIEIDFKHPNLYQRYNARGKDVMMPEVSFKDFRKGSQFFILNRNHAKVVLRDQKLWKKFRIPCIDKSSCYPEEHYFPTLLSMEDPDGCTGFTLTRVNWTGCTDHPHLYTREEVSPELVRQLRKSDFSYSYLFARKFSPETLAPLMDIAYDVIFRD