AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53743

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53743.t1 MTR_1g031080 83.188 345 5 1 1 345 1 292 0.0 564
MS.gene53743.t1 MTR_1g031060 81.503 346 63 1 1 345 17 362 0.0 514
MS.gene53743.t1 MTR_1g048320 74.352 347 80 5 4 345 2 344 4.64e-174 487
MS.gene53743.t1 MTR_2g087610 52.830 371 146 7 1 345 1 368 1.96e-116 342
MS.gene53743.t1 MTR_3g106930 52.189 297 113 4 64 345 95 377 2.39e-94 286
MS.gene53743.t1 MTR_1g110290 35.312 320 174 10 33 342 95 391 3.40e-48 167
MS.gene53743.t1 MTR_2g005350 37.241 290 151 7 63 342 168 436 4.04e-48 168
MS.gene53743.t1 MTR_1g009960 39.163 263 132 11 63 319 111 351 4.85e-47 163
MS.gene53743.t1 MTR_1g009960 38.206 301 137 13 63 335 111 390 1.61e-46 162
MS.gene53743.t1 MTR_3g108740 34.848 330 166 10 34 335 63 371 1.65e-46 162
MS.gene53743.t1 MTR_8g015500 34.602 289 160 7 63 342 143 411 3.47e-46 162
MS.gene53743.t1 MTR_1g016880 34.256 289 160 7 63 342 106 373 6.61e-46 160
MS.gene53743.t1 MTR_2g037600 34.237 295 149 6 61 329 103 378 1.15e-45 160
MS.gene53743.t1 MTR_4g127550 33.553 304 156 8 58 335 102 385 1.73e-45 160
MS.gene53743.t1 MTR_5g006930 32.808 317 179 9 37 342 91 384 3.40e-45 159
MS.gene53743.t1 MTR_5g055480 36.770 291 149 9 63 342 122 388 1.02e-44 157
MS.gene53743.t1 MTR_5g006920 34.494 316 175 10 37 342 126 419 1.47e-44 158
MS.gene53743.t1 MTR_2g037620 32.320 362 195 9 6 337 36 377 2.32e-44 157
MS.gene53743.t1 MTR_6g083830 33.123 317 178 9 37 342 124 417 8.13e-43 153
MS.gene53743.t1 MTR_3g104840 33.016 315 163 7 37 342 116 391 3.92e-41 148
MS.gene53743.t1 MTR_2g024120 31.902 326 154 12 63 342 61 364 2.25e-31 122
MS.gene53743.t1 MTR_8g094180 31.228 285 137 10 62 312 54 313 3.65e-29 115
MS.gene53743.t1 MTR_8g094180 29.341 334 154 12 62 338 54 362 3.88e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53743.t1 AT3G52060 58.044 317 112 4 37 345 43 346 8.52e-132 380
MS.gene53743.t1 AT5G22070 52.762 362 154 8 1 345 1 362 1.66e-130 377
MS.gene53743.t1 AT5G25330 48.852 305 122 7 60 345 73 362 1.17e-87 268
MS.gene53743.t1 AT4G32290 49.169 301 120 4 64 345 93 379 7.78e-86 264
MS.gene53743.t1 AT4G31350 35.905 337 168 9 26 335 51 366 1.08e-53 181
MS.gene53743.t1 AT4G31350 35.905 337 168 9 26 335 51 366 1.08e-53 181
MS.gene53743.t1 AT4G31350 35.905 337 168 9 26 335 51 366 1.08e-53 181
MS.gene53743.t1 AT4G31350 35.905 337 163 10 26 335 51 361 5.65e-52 176
MS.gene53743.t1 AT5G25970 35.959 292 157 8 60 342 115 385 1.13e-49 171
MS.gene53743.t1 AT5G25970 35.959 292 157 8 60 342 115 385 1.13e-49 171
MS.gene53743.t1 AT5G25970 35.959 292 157 8 60 342 115 385 1.13e-49 171
MS.gene53743.t1 AT1G51770 35.294 289 157 7 63 342 123 390 7.18e-48 166
MS.gene53743.t1 AT5G11730 34.948 289 158 7 63 342 117 384 2.58e-47 164
MS.gene53743.t1 AT4G30060 35.548 301 147 8 64 337 109 389 1.22e-46 163
MS.gene53743.t1 AT4G30060 35.548 301 147 8 64 337 109 389 1.22e-46 163
MS.gene53743.t1 AT1G10280 36.897 290 152 9 63 342 142 410 5.83e-46 161
MS.gene53743.t1 AT2G19160 36.213 301 145 9 64 337 106 386 6.33e-46 161
MS.gene53743.t1 AT2G19160 36.213 301 145 9 64 337 106 386 6.33e-46 161
MS.gene53743.t1 AT3G21310 35.640 289 156 8 63 342 114 381 9.57e-46 160
MS.gene53743.t1 AT3G21310 35.640 289 156 8 63 342 114 381 9.57e-46 160
MS.gene53743.t1 AT1G10880 33.442 308 173 9 40 338 96 380 2.96e-41 148
MS.gene53743.t1 AT4G25870 33.993 303 150 9 64 337 99 380 4.26e-41 148
MS.gene53743.t1 AT5G16170 33.220 295 158 8 64 342 136 407 9.87e-41 147
MS.gene53743.t1 AT5G57270 35.000 280 150 8 62 333 96 351 1.03e-40 147
MS.gene53743.t1 AT5G57270 35.000 280 150 8 62 333 96 351 1.03e-40 147
MS.gene53743.t1 AT5G57270 35.000 280 150 8 62 333 96 351 1.03e-40 147
MS.gene53743.t1 AT5G57270 35.000 280 150 8 62 333 96 351 1.03e-40 147
MS.gene53743.t1 AT5G57270 35.000 280 150 8 62 333 96 351 1.03e-40 147
MS.gene53743.t1 AT1G68380 33.851 322 170 11 37 343 98 391 2.18e-38 141
MS.gene53743.t1 AT1G68390 32.302 291 165 7 63 342 137 406 2.25e-38 141
MS.gene53743.t1 AT1G51770 32.526 289 138 8 63 342 123 363 1.23e-35 133
MS.gene53743.t1 AT1G73810 34.915 295 151 10 64 342 147 416 2.04e-34 130
MS.gene53743.t1 AT5G14550 29.373 303 170 8 42 324 43 321 9.34e-33 125
MS.gene53743.t1 AT5G14550 27.299 348 177 12 42 337 43 366 2.15e-29 116
MS.gene53743.t1 AT5G14550 26.994 326 180 10 42 333 43 344 4.51e-29 115
MS.gene53743.t1 AT1G11940 30.233 344 167 15 47 342 57 375 9.21e-29 115
MS.gene53743.t1 AT1G62305 30.312 320 153 12 55 327 60 356 3.49e-27 110
MS.gene53743.t1 AT1G62305 32.068 237 120 8 55 271 60 275 7.54e-23 97.4
MS.gene53743.t1 AT5G14550 26.946 334 169 13 42 337 43 339 2.37e-22 96.7
MS.gene53743.t1 AT3G21310 41.322 121 69 2 63 182 114 233 4.02e-22 94.4
MS.gene53743.t1 AT1G11940 33.649 211 114 8 47 252 57 246 1.85e-21 92.8

Find 103 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCACCTTTGCTCCTTTA+TGG 0.074069 1.3:-30351352 MS.gene53743:CDS
TCGGATTCCTTGTGTAAATT+TGG 0.194070 1.3:-30350829 MS.gene53743:CDS
CCTCACCTTTGCTCCTTTAT+GGG 0.265226 1.3:-30351351 MS.gene53743:CDS
TCACACTGACGAGAGTTAAT+TGG 0.269052 1.3:-30350734 MS.gene53743:CDS
TGGATAAGTGGGTGGTGGTG+TGG 0.269984 1.3:+30351426 None:intergenic
GTTGAAGAGATGGTTGTTTC+CGG 0.278745 1.3:+30351316 None:intergenic
AGGGGATACGACGGAGGTGG+TGG 0.323021 1.3:+30351274 None:intergenic
GGATAAGTGGGTGGTGGTGT+GGG 0.332097 1.3:+30351427 None:intergenic
GTCGGAAAATAATGCTCTTC+TGG 0.337675 1.3:+30350795 None:intergenic
ATAAGCTCTGGAAGAAGTTT+CGG 0.354353 1.3:-30350848 MS.gene53743:CDS
GGAGGTGTTGGGAGAGGAAG+AGG 0.356101 1.3:+30351516 None:intergenic
TAAGTGGGTGGTGGTGTGGG+TGG 0.365938 1.3:+30351430 None:intergenic
TGGAAGAGATGAAACGGTTA+TGG 0.369541 1.3:+30351240 None:intergenic
AGGGCCAGGTCATCGGCGTC+GGG 0.373907 1.3:+30351467 None:intergenic
GTGGGTGGTGGTGTGGGTGG+TGG 0.377134 1.3:+30351433 None:intergenic
GGGTGGTGGCACGGTGGAAG+AGG 0.389635 1.3:+30351447 None:intergenic
AGGTTTAGGGTTTGTGAGGG+TGG 0.408760 1.3:+30351406 None:intergenic
TGAATTGTGGGAGGAGGTGT+TGG 0.410675 1.3:+30351504 None:intergenic
GGAGTGTCTTGCTCCGTTGA+TGG 0.414094 1.3:-30350589 MS.gene53743:CDS
GGTTTGTGAGGGTGGATAAG+TGG 0.419000 1.3:+30351414 None:intergenic
TGGAGAGAATGTTATGTTGC+CGG 0.421375 1.3:-30350952 MS.gene53743:CDS
GGTTAGTGTTTGTGAGGAAG+AGG 0.432579 1.3:+30351372 None:intergenic
GTTTGTGAGGGTGGATAAGT+GGG 0.436623 1.3:+30351415 None:intergenic
GTTGCCGGAGGTTCCGTTTG+AGG 0.437713 1.3:-30350937 MS.gene53743:CDS
GAGGGCCAGGTCATCGGCGT+CGG 0.438937 1.3:+30351466 None:intergenic
GTGGGAGGAGGTGTTGGGAG+AGG 0.439414 1.3:+30351510 None:intergenic
GAGTTAATTGGACTGGTTGT+TGG 0.442362 1.3:-30350722 MS.gene53743:CDS
GGGATACGACGGAGGTGGTG+GGG 0.452133 1.3:+30351276 None:intergenic
AAAGCAAAGTATTGGTTAAG+TGG 0.453008 1.3:+30351143 None:intergenic
CTCAGCTGTCGAATTAGCTC+CGG 0.455031 1.3:+30350660 None:intergenic
TGGTGGTGTGGGTGGTGGCA+CGG 0.462193 1.3:+30351438 None:intergenic
AGTTAATTGGACTGGTTGTT+GGG 0.463114 1.3:-30350721 MS.gene53743:CDS
AGAGCTAGAGAGACAAGAAT+TGG 0.468069 1.3:+30351560 None:intergenic
TCAGCTGTCGAATTAGCTCC+GGG 0.469076 1.3:+30350661 None:intergenic
GAGAGAATCTCAATATAGCT+AGG 0.471455 1.3:+30351011 None:intergenic
TGGCACGGTGGAAGAGGGCC+AGG 0.471718 1.3:+30351453 None:intergenic
GAGAAACTAAAGCAAAGTAT+TGG 0.471858 1.3:+30351135 None:intergenic
CGGAGGTGGTGGGGTCTGCA+TGG 0.475322 1.3:+30351285 None:intergenic
TACTCCAGAGGAGGTTTCCC+CGG 0.476354 1.3:-30350679 MS.gene53743:CDS
ACCACCTCCGTCGTATCCCC+TGG 0.479754 1.3:-30351272 MS.gene53743:CDS
GGGAGAGGAAGAGGACGAGT+GGG 0.485804 1.3:+30351525 None:intergenic
GGGGATACGACGGAGGTGGT+GGG 0.489281 1.3:+30351275 None:intergenic
TGAAGCACGTTGTGTTGGTT+TGG 0.493511 1.3:+30351220 None:intergenic
CGGTTATGGAAGACACCGCC+AGG 0.500731 1.3:+30351254 None:intergenic
AGCTCCGGGGAAACCTCCTC+TGG 0.502418 1.3:+30350675 None:intergenic
GGTGGTGGCACGGTGGAAGA+GGG 0.504324 1.3:+30351448 None:intergenic
TGGTGAGAGATTATAAGCTC+TGG 0.506455 1.3:-30350860 MS.gene53743:CDS
GTGGAAGAGGGCCAGGTCAT+CGG 0.508067 1.3:+30351460 None:intergenic
TTGTAACGCTCGTAGAGATT+CGG 0.510630 1.3:+30350981 None:intergenic
GAATTGTGGGAGGAGGTGTT+GGG 0.511026 1.3:+30351505 None:intergenic
TTGTCAATGGAGGATCTGAA+TGG 0.519606 1.3:-30350768 MS.gene53743:CDS
GGCCAGGTCATCGGCGTCGG+GGG 0.519778 1.3:+30351469 None:intergenic
TGGGAGAGGAAGAGGACGAG+TGG 0.530108 1.3:+30351524 None:intergenic
GGACGAGTGGGAGAGAGATG+AGG 0.537328 1.3:+30351537 None:intergenic
AAATCCTCAAACGGAACCTC+CGG 0.539492 1.3:+30350933 None:intergenic
AGAAAGGAAAGGTGAATTGT+GGG 0.540874 1.3:+30351492 None:intergenic
GAGGGTGGATAAGTGGGTGG+TGG 0.541141 1.3:+30351421 None:intergenic
AGGTGAGGTTAGTGTTTGTG+AGG 0.541730 1.3:+30351366 None:intergenic
TGTCAATGGAGGATCTGAAT+GGG 0.561186 1.3:-30350767 MS.gene53743:CDS
CACCGAGCAAGCTTCAATCC+CGG 0.562100 1.3:+30350544 None:intergenic
GGGCCAGGTCATCGGCGTCG+GGG 0.567095 1.3:+30351468 None:intergenic
CTGACGAGAGTTAATTGGAC+TGG 0.567457 1.3:-30350729 MS.gene53743:CDS
GAGGATCTGAATGGGTGTAC+TGG 0.569583 1.3:-30350759 MS.gene53743:CDS
AATTGGACTGGTTGTTGGGA+TGG 0.570319 1.3:-30350717 MS.gene53743:CDS
CCAACCCGAAAATCCTCAAA+CGG 0.573571 1.3:+30350924 None:intergenic
TTTAGGTTTAGGGTTTGTGA+GGG 0.574889 1.3:+30351403 None:intergenic
AGAATTGGTGATGACAACAT+TGG 0.580475 1.3:+30351575 None:intergenic
GACACCGCCAGGGGATACGA+CGG 0.582306 1.3:+30351265 None:intergenic
GATGGTGAAGCACGTTGTGT+TGG 0.582509 1.3:+30351215 None:intergenic
CCCATAAAGGAGCAAAGGTG+AGG 0.584315 1.3:+30351351 None:intergenic
GCCAGGGGATACGACGGAGG+TGG 0.585534 1.3:+30351271 None:intergenic
GAGAAAGGAAAGGTGAATTG+TGG 0.586906 1.3:+30351491 None:intergenic
TGTGAGGGTGGATAAGTGGG+TGG 0.589818 1.3:+30351418 None:intergenic
TCCTCCATTGACAAAAGCGT+CGG 0.590314 1.3:+30350777 None:intergenic
CTTGAACAGGAAGCATGCAA+AGG 0.593306 1.3:-30350886 MS.gene53743:CDS
GAAAGGTGAATTGTGGGAGG+AGG 0.594674 1.3:+30351498 None:intergenic
GTTATTCGTATTTATTTGCG+AGG 0.596149 1.3:-30350623 MS.gene53743:CDS
CGGAGCTAATTCGACAGCTG+AGG 0.597295 1.3:-30350659 MS.gene53743:CDS
TTGGTTTGGAAGAGATGAAA+CGG 0.597722 1.3:+30351234 None:intergenic
TGCTATGAGACGTCGTGCAG+CGG 0.599661 1.3:+30351184 None:intergenic
AGAGAATCTCAATATAGCTA+GGG 0.599756 1.3:+30351012 None:intergenic
GGATGTATATGTTGAAGAGA+TGG 0.601004 1.3:+30351306 None:intergenic
TCATCTTTATACTCCAGAGG+AGG 0.602098 1.3:-30350688 MS.gene53743:CDS
GGAGCTAATTCGACAGCTGA+GGG 0.603180 1.3:-30350658 MS.gene53743:CDS
CAGGAAGCATGCAAAGGTTG+TGG 0.603895 1.3:-30350880 MS.gene53743:CDS
TCCTCTGGAGTATAAAGATG+AGG 0.605118 1.3:+30350690 None:intergenic
TACGAGCGTTACAACGCTCG+TGG 0.612071 1.3:-30350972 MS.gene53743:CDS
AAGGAAAGGTGAATTGTGGG+AGG 0.614891 1.3:+30351495 None:intergenic
TCCTCATCTTTATACTCCAG+AGG 0.615195 1.3:-30350691 MS.gene53743:CDS
ATCAACGGAGCAAGACACTC+CGG 0.628501 1.3:+30350591 None:intergenic
ATTTGCGAGGAAATTCTCGC+CGG 0.634389 1.3:-30350610 MS.gene53743:CDS
CAAGAATCCAAATTTACACA+AGG 0.644692 1.3:+30350822 None:intergenic
TGGTGTGGGTGGTGGCACGG+TGG 0.645982 1.3:+30351441 None:intergenic
CTATATTGAGATTCTCTCTG+AGG 0.647208 1.3:-30351006 MS.gene53743:CDS
TCATCAGCTATATCCATCAA+CGG 0.652852 1.3:+30350576 None:intergenic
AGAGAATGTTATGTTGCCGG+AGG 0.659698 1.3:-30350949 MS.gene53743:CDS
GGTTATGGAAGACACCGCCA+GGG 0.669186 1.3:+30351255 None:intergenic
ACGAATCGAAACGACAAGAG+CGG 0.691362 1.3:+30351101 None:intergenic
ACCTCCGTCGTATCCCCTGG+CGG 0.701974 1.3:-30351269 MS.gene53743:CDS
ACCGCCAGGGGATACGACGG+AGG 0.706931 1.3:+30351268 None:intergenic
CGTGCAGCGGAGATGAGTGA+TGG 0.712720 1.3:+30351197 None:intergenic
CAGCTGTCGAATTAGCTCCG+GGG 0.758894 1.3:+30350662 None:intergenic
GTTATGGAAGACACCGCCAG+GGG 0.770600 1.3:+30351256 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAATAACTGATTTTTAAAA+AGG + chr1.3:30351063-30351082 None:intergenic 15.0%
AGAGAATCTCAATATAGCTA+GGG + chr1.3:30351120-30351139 None:intergenic 30.0%
CAAGAATCCAAATTTACACA+AGG + chr1.3:30351310-30351329 None:intergenic 30.0%
GAGAAACTAAAGCAAAGTAT+TGG + chr1.3:30350997-30351016 None:intergenic 30.0%
GTTATTCGTATTTATTTGCG+AGG - chr1.3:30351506-30351525 MS.gene53743:CDS 30.0%
!! AAAGCAAAGTATTGGTTAAG+TGG + chr1.3:30350989-30351008 None:intergenic 30.0%
AGAAAGGAAAGGTGAATTGT+GGG + chr1.3:30350640-30350659 None:intergenic 35.0%
CTATATTGAGATTCTCTCTG+AGG - chr1.3:30351123-30351142 MS.gene53743:CDS 35.0%
GAGAGAATCTCAATATAGCT+AGG + chr1.3:30351121-30351140 None:intergenic 35.0%
GGATGTATATGTTGAAGAGA+TGG + chr1.3:30350826-30350845 None:intergenic 35.0%
TCATCAGCTATATCCATCAA+CGG + chr1.3:30351556-30351575 None:intergenic 35.0%
TCGGATTCCTTGTGTAAATT+TGG - chr1.3:30351300-30351319 MS.gene53743:CDS 35.0%
TTATGGGAAAAATTCTTCGC+CGG - chr1.3:30350794-30350813 MS.gene53743:CDS 35.0%
! GCGAAGAATTTTTCCCATAA+AGG + chr1.3:30350794-30350813 None:intergenic 35.0%
! TTTAGGTTTAGGGTTTGTGA+GGG + chr1.3:30350729-30350748 None:intergenic 35.0%
! TTTTTCCCATAAAGGAGCAA+AGG + chr1.3:30350786-30350805 None:intergenic 35.0%
!! AGTTAATTGGACTGGTTGTT+GGG - chr1.3:30351408-30351427 MS.gene53743:CDS 35.0%
!! ATAAGCTCTGGAAGAAGTTT+CGG - chr1.3:30351281-30351300 MS.gene53743:CDS 35.0%
!! TTGGTTTGGAAGAGATGAAA+CGG + chr1.3:30350898-30350917 None:intergenic 35.0%
!!! AGGAAAGCGATTTTAGGTTT+AGG + chr1.3:30350740-30350759 None:intergenic 35.0%
!!! CGCAGTTTTTTATCTTGAAC+AGG - chr1.3:30351230-30351249 MS.gene53743:CDS 35.0%
!!! GGAAAGCGATTTTAGGTTTA+GGG + chr1.3:30350739-30350758 None:intergenic 35.0%
!!! TAGCTGATGATGTCATTTTC+CGG - chr1.3:30351566-30351585 MS.gene53743:CDS 35.0%
!!! TTTTAGGTTTAGGGTTTGTG+AGG + chr1.3:30350730-30350749 None:intergenic 35.0%
AGAGCTAGAGAGACAAGAAT+TGG + chr1.3:30350572-30350591 None:intergenic 40.0%
GAGAAAGGAAAGGTGAATTG+TGG + chr1.3:30350641-30350660 None:intergenic 40.0%
GTCGGAAAATAATGCTCTTC+TGG + chr1.3:30351337-30351356 None:intergenic 40.0%
TCACACTGACGAGAGTTAAT+TGG - chr1.3:30351395-30351414 MS.gene53743:CDS 40.0%
TCATCTTTATACTCCAGAGG+AGG - chr1.3:30351441-30351460 MS.gene53743:CDS 40.0%
TCCTCATCTTTATACTCCAG+AGG - chr1.3:30351438-30351457 MS.gene53743:CDS 40.0%
TGGAAGAGATGAAACGGTTA+TGG + chr1.3:30350892-30350911 None:intergenic 40.0%
TGGAGAGAATGTTATGTTGC+CGG - chr1.3:30351177-30351196 MS.gene53743:CDS 40.0%
TGGTGAGAGATTATAAGCTC+TGG - chr1.3:30351269-30351288 MS.gene53743:CDS 40.0%
TGTCAATGGAGGATCTGAAT+GGG - chr1.3:30351362-30351381 MS.gene53743:CDS 40.0%
TTGTAACGCTCGTAGAGATT+CGG + chr1.3:30351151-30351170 None:intergenic 40.0%
TTGTCAATGGAGGATCTGAA+TGG - chr1.3:30351361-30351380 MS.gene53743:CDS 40.0%
! AGGAAGAGGAAAGCGATTTT+AGG + chr1.3:30350746-30350765 None:intergenic 40.0%
! AGGTTCCGTTTGAGGATTTT+CGG - chr1.3:30351200-30351219 MS.gene53743:CDS 40.0%
! GTTGAAGAGATGGTTGTTTC+CGG + chr1.3:30350816-30350835 None:intergenic 40.0%
! TTTTCCGACGCTTTTGTCAA+TGG - chr1.3:30351348-30351367 MS.gene53743:CDS 40.0%
!! GAGTTAATTGGACTGGTTGT+TGG - chr1.3:30351407-30351426 MS.gene53743:CDS 40.0%
!! TCCTCTGGAGTATAAAGATG+AGG + chr1.3:30351442-30351461 None:intergenic 40.0%
!!! AGCTGATGATGTCATTTTCC+GGG - chr1.3:30351567-30351586 MS.gene53743:CDS 40.0%
AAATCCTCAAACGGAACCTC+CGG + chr1.3:30351199-30351218 None:intergenic 45.0%
AAGGAAAGGTGAATTGTGGG+AGG + chr1.3:30350637-30350656 None:intergenic 45.0%
ACCTCACCTTTGCTCCTTTA+TGG - chr1.3:30350777-30350796 MS.gene53743:CDS 45.0%
ACGAATCGAAACGACAAGAG+CGG + chr1.3:30351031-30351050 None:intergenic 45.0%
AGAGAATGTTATGTTGCCGG+AGG - chr1.3:30351180-30351199 MS.gene53743:CDS 45.0%
AGGTGAGGTTAGTGTTTGTG+AGG + chr1.3:30350766-30350785 None:intergenic 45.0%
ATTTGCGAGGAAATTCTCGC+CGG - chr1.3:30351519-30351538 MS.gene53743:CDS 45.0%
CCAACCCGAAAATCCTCAAA+CGG + chr1.3:30351208-30351227 None:intergenic 45.0%
CCTCACCTTTGCTCCTTTAT+GGG - chr1.3:30350778-30350797 MS.gene53743:CDS 45.0%
CTGACGAGAGTTAATTGGAC+TGG - chr1.3:30351400-30351419 MS.gene53743:CDS 45.0%
GGTTAGTGTTTGTGAGGAAG+AGG + chr1.3:30350760-30350779 None:intergenic 45.0%
GTTTGTGAGGGTGGATAAGT+GGG + chr1.3:30350717-30350736 None:intergenic 45.0%
! CTTGAACAGGAAGCATGCAA+AGG - chr1.3:30351243-30351262 MS.gene53743:CDS 45.0%
! GGTTCCGTTTGAGGATTTTC+GGG - chr1.3:30351201-30351220 MS.gene53743:CDS 45.0%
! TCCTCCATTGACAAAAGCGT+CGG + chr1.3:30351355-30351374 None:intergenic 45.0%
!! AATTGGACTGGTTGTTGGGA+TGG - chr1.3:30351412-30351431 MS.gene53743:CDS 45.0%
!! TGAAGCACGTTGTGTTGGTT+TGG + chr1.3:30350912-30350931 None:intergenic 45.0%
ATCAACGGAGCAAGACACTC+CGG + chr1.3:30351541-30351560 None:intergenic 50.0%
CCCATAAAGGAGCAAAGGTG+AGG + chr1.3:30350781-30350800 None:intergenic 50.0%
CTCAGCTGTCGAATTAGCTC+CGG + chr1.3:30351472-30351491 None:intergenic 50.0%
GAAAGGTGAATTGTGGGAGG+AGG + chr1.3:30350634-30350653 None:intergenic 50.0%
GAGGATCTGAATGGGTGTAC+TGG - chr1.3:30351370-30351389 MS.gene53743:CDS 50.0%
GATGGTGAAGCACGTTGTGT+TGG + chr1.3:30350917-30350936 None:intergenic 50.0%
GGAGCTAATTCGACAGCTGA+GGG - chr1.3:30351471-30351490 MS.gene53743:CDS 50.0%
GGTTTGTGAGGGTGGATAAG+TGG + chr1.3:30350718-30350737 None:intergenic 50.0%
TCAGCTGTCGAATTAGCTCC+GGG + chr1.3:30351471-30351490 None:intergenic 50.0%
! AGGTTTAGGGTTTGTGAGGG+TGG + chr1.3:30350726-30350745 None:intergenic 50.0%
! CAGGAAGCATGCAAAGGTTG+TGG - chr1.3:30351249-30351268 MS.gene53743:CDS 50.0%
! TCCGACGCTTTTGTCAATGG+AGG - chr1.3:30351351-30351370 MS.gene53743:CDS 50.0%
! TGAATTGTGGGAGGAGGTGT+TGG + chr1.3:30350628-30350647 None:intergenic 50.0%
!! GAATTGTGGGAGGAGGTGTT+GGG + chr1.3:30350627-30350646 None:intergenic 50.0%
!!! CCGTTTGAGGATTTTCGGGT+TGG - chr1.3:30351205-30351224 MS.gene53743:CDS 50.0%
CAGCTGTCGAATTAGCTCCG+GGG + chr1.3:30351470-30351489 None:intergenic 55.0%
CGGAGCTAATTCGACAGCTG+AGG - chr1.3:30351470-30351489 MS.gene53743:CDS 55.0%
GGTTATGGAAGACACCGCCA+GGG + chr1.3:30350877-30350896 None:intergenic 55.0%
GTTATGGAAGACACCGCCAG+GGG + chr1.3:30350876-30350895 None:intergenic 55.0%
TACGAGCGTTACAACGCTCG+TGG - chr1.3:30351157-30351176 MS.gene53743:CDS 55.0%
TACTCCAGAGGAGGTTTCCC+CGG - chr1.3:30351450-30351469 MS.gene53743:CDS 55.0%
TGCTATGAGACGTCGTGCAG+CGG + chr1.3:30350948-30350967 None:intergenic 55.0%
TGTGAGGGTGGATAAGTGGG+TGG + chr1.3:30350714-30350733 None:intergenic 55.0%
! GGAGTGTCTTGCTCCGTTGA+TGG - chr1.3:30351540-30351559 MS.gene53743:CDS 55.0%
! GGATAAGTGGGTGGTGGTGT+GGG + chr1.3:30350705-30350724 None:intergenic 55.0%
! TGGATAAGTGGGTGGTGGTG+TGG + chr1.3:30350706-30350725 None:intergenic 55.0%
CGGTTATGGAAGACACCGCC+AGG + chr1.3:30350878-30350897 None:intergenic 60.0%
CGTGCAGCGGAGATGAGTGA+TGG + chr1.3:30350935-30350954 None:intergenic 60.0%
GAGGGTGGATAAGTGGGTGG+TGG + chr1.3:30350711-30350730 None:intergenic 60.0%
GGACGAGTGGGAGAGAGATG+AGG + chr1.3:30350595-30350614 None:intergenic 60.0%
GGGAGAGGAAGAGGACGAGT+GGG + chr1.3:30350607-30350626 None:intergenic 60.0%
GTGGAAGAGGGCCAGGTCAT+CGG + chr1.3:30350672-30350691 None:intergenic 60.0%
GTTGCCGGAGGTTCCGTTTG+AGG - chr1.3:30351192-30351211 MS.gene53743:CDS 60.0%
TGGGAGAGGAAGAGGACGAG+TGG + chr1.3:30350608-30350627 None:intergenic 60.0%
! TAAGTGGGTGGTGGTGTGGG+TGG + chr1.3:30350702-30350721 None:intergenic 60.0%
!! GGAGGTGTTGGGAGAGGAAG+AGG + chr1.3:30350616-30350635 None:intergenic 60.0%
ACCACCTCCGTCGTATCCCC+TGG - chr1.3:30350857-30350876 MS.gene53743:CDS 65.0%
ACCTCCGTCGTATCCCCTGG+CGG - chr1.3:30350860-30350879 MS.gene53743:CDS 65.0%
AGCTCCGGGGAAACCTCCTC+TGG + chr1.3:30351457-30351476 None:intergenic 65.0%
AGGGGATACGACGGAGGTGG+TGG + chr1.3:30350858-30350877 None:intergenic 65.0%
GACACCGCCAGGGGATACGA+CGG + chr1.3:30350867-30350886 None:intergenic 65.0%
GGGATACGACGGAGGTGGTG+GGG + chr1.3:30350856-30350875 None:intergenic 65.0%
GGGGATACGACGGAGGTGGT+GGG + chr1.3:30350857-30350876 None:intergenic 65.0%
! GGCGTCGGGGGAGAAAGGAA+AGG + chr1.3:30350651-30350670 None:intergenic 65.0%
! GGTGGTGGCACGGTGGAAGA+GGG + chr1.3:30350684-30350703 None:intergenic 65.0%
! TCATCGGCGTCGGGGGAGAA+AGG + chr1.3:30350656-30350675 None:intergenic 65.0%
! TGGTGGTGTGGGTGGTGGCA+CGG + chr1.3:30350694-30350713 None:intergenic 65.0%
!! GTGGGAGGAGGTGTTGGGAG+AGG + chr1.3:30350622-30350641 None:intergenic 65.0%
ACCGCCAGGGGATACGACGG+AGG + chr1.3:30350864-30350883 None:intergenic 70.0%
AGGGCCAGGTCATCGGCGTC+GGG + chr1.3:30350665-30350684 None:intergenic 70.0%
GAGGGCCAGGTCATCGGCGT+CGG + chr1.3:30350666-30350685 None:intergenic 70.0%
GCCAGGGGATACGACGGAGG+TGG + chr1.3:30350861-30350880 None:intergenic 70.0%
TGGCACGGTGGAAGAGGGCC+AGG + chr1.3:30350679-30350698 None:intergenic 70.0%
! GGGTGGTGGCACGGTGGAAG+AGG + chr1.3:30350685-30350704 None:intergenic 70.0%
! GTGGGTGGTGGTGTGGGTGG+TGG + chr1.3:30350699-30350718 None:intergenic 70.0%
! TGGTGTGGGTGGTGGCACGG+TGG + chr1.3:30350691-30350710 None:intergenic 70.0%
!! CGGAGGTGGTGGGGTCTGCA+TGG + chr1.3:30350847-30350866 None:intergenic 70.0%
CTCCCCCGACGCCGATGACC+TGG - chr1.3:30350658-30350677 MS.gene53743:CDS 75.0%
GGGCCAGGTCATCGGCGTCG+GGG + chr1.3:30350664-30350683 None:intergenic 75.0%
! GGCCAGGTCATCGGCGTCGG+GGG + chr1.3:30350663-30350682 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 30350557 30351594 30350557 ID=MS.gene53743
chr1.3 mRNA 30350557 30351594 30350557 ID=MS.gene53743.t1;Parent=MS.gene53743
chr1.3 exon 30350557 30351594 30350557 ID=MS.gene53743.t1.exon1;Parent=MS.gene53743.t1
chr1.3 CDS 30350557 30351594 30350557 ID=cds.MS.gene53743.t1;Parent=MS.gene53743.t1
Gene Sequence

>MS.gene53743

ATGTTGTCATCACCAATTCTTGTCTCTCTAGCTCTCCTCATCTCTCTCCCACTCGTCCTCTTCCTCTCCCAACACCTCCTCCCACAATTCACCTTTCCTTTCTCCCCCGACGCCGATGACCTGGCCCTCTTCCACCGTGCCACCACCCACACCACCACCCACTTATCCACCCTCACAAACCCTAAACCTAAAATCGCTTTCCTCTTCCTCACAAACACTAACCTCACCTTTGCTCCTTTATGGGAAAAATTCTTCGCCGGAAACAACCATCTCTTCAACATATACATCCATGCAGACCCCACCACCTCCGTCGTATCCCCTGGCGGTGTCTTCCATAACCGTTTCATCTCTTCCAAACCAACACAACGTGCTTCACCATCACTCATCTCCGCTGCACGACGTCTCATAGCATCTGCTCTCTTAGATGATCCACTTAACCAATACTTTGCTTTAGTTTCTCAACACTGTGTTCCGCTCTTGTCGTTTCGATTCGTTTATAACTACCTTTTTAAAAATCAGTTATTGTCGTTAGCGAGTTTCTCTGACTTTAACTTGCTTTACCCTAGCTATATTGAGATTCTCTCTGAGGATCCGAATCTCTACGAGCGTTACAACGCTCGTGGAGAGAATGTTATGTTGCCGGAGGTTCCGTTTGAGGATTTTCGGGTTGGTTCGCAGTTTTTTATCTTGAACAGGAAGCATGCAAAGGTTGTGGTGAGAGATTATAAGCTCTGGAAGAAGTTTCGGATTCCTTGTGTAAATTTGGATTCTTGTTATCCAGAAGAGCATTATTTTCCGACGCTTTTGTCAATGGAGGATCTGAATGGGTGTACTGGTTTCACACTGACGAGAGTTAATTGGACTGGTTGTTGGGATGGACATCCTCATCTTTATACTCCAGAGGAGGTTTCCCCGGAGCTAATTCGACAGCTGAGGGTGTCAAATTCGAGTTATTCGTATTTATTTGCGAGGAAATTCTCGCCGGAGTGTCTTGCTCCGTTGATGGATATAGCTGATGATGTCATTTTCCGGGATTGA
Protein sequence

>MS.gene53743.t1

MLSSPILVSLALLISLPLVLFLSQHLLPQFTFPFSPDADDLALFHRATTHTTTHLSTLTNPKPKIAFLFLTNTNLTFAPLWEKFFAGNNHLFNIYIHADPTTSVVSPGGVFHNRFISSKPTQRASPSLISAARRLIASALLDDPLNQYFALVSQHCVPLLSFRFVYNYLFKNQLLSLASFSDFNLLYPSYIEILSEDPNLYERYNARGENVMLPEVPFEDFRVGSQFFILNRKHAKVVVRDYKLWKKFRIPCVNLDSCYPEEHYFPTLLSMEDLNGCTGFTLTRVNWTGCWDGHPHLYTPEEVSPELIRQLRVSNSSYSYLFARKFSPECLAPLMDIADDVIFRD