AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53744

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53744.t1 MTR_1g031060 95.028 362 18 0 1 362 1 362 0.0 640
MS.gene53744.t1 MTR_1g031080 67.630 346 58 2 17 362 1 292 4.30e-161 453
MS.gene53744.t1 MTR_1g048320 68.103 348 101 5 18 362 4 344 8.84e-154 436
MS.gene53744.t1 MTR_2g087610 56.081 296 117 5 78 362 75 368 6.53e-104 310
MS.gene53744.t1 MTR_3g106930 50.168 297 119 5 81 362 95 377 6.40e-87 267
MS.gene53744.t1 MTR_5g006930 37.386 329 173 12 38 355 74 380 1.55e-47 166
MS.gene53744.t1 MTR_5g006920 36.474 329 176 12 38 355 109 415 1.02e-46 164
MS.gene53744.t1 MTR_1g110290 36.593 317 166 11 54 359 99 391 1.80e-44 158
MS.gene53744.t1 MTR_5g055480 36.392 316 167 10 54 359 97 388 1.93e-43 155
MS.gene53744.t1 MTR_2g037600 33.225 307 160 8 74 354 99 386 2.64e-43 154
MS.gene53744.t1 MTR_6g083830 34.925 335 185 11 36 359 105 417 6.85e-43 154
MS.gene53744.t1 MTR_2g037620 30.867 392 202 15 9 354 9 377 2.49e-42 152
MS.gene53744.t1 MTR_1g009960 37.405 262 138 9 80 336 111 351 2.94e-42 151
MS.gene53744.t1 MTR_4g127550 32.573 307 159 9 75 354 102 387 7.61e-42 150
MS.gene53744.t1 MTR_3g108740 36.333 300 144 9 80 352 92 371 9.84e-42 150
MS.gene53744.t1 MTR_1g009960 37.402 254 133 9 80 328 111 343 6.44e-41 148
MS.gene53744.t1 MTR_8g015500 33.866 313 177 8 56 359 120 411 1.53e-40 147
MS.gene53744.t1 MTR_2g005350 34.354 294 154 8 80 359 168 436 1.80e-40 148
MS.gene53744.t1 MTR_3g104840 34.810 316 158 9 49 355 111 387 6.34e-40 145
MS.gene53744.t1 MTR_1g016880 33.333 291 164 8 80 361 106 375 1.09e-39 144
MS.gene53744.t1 MTR_2g024120 30.368 326 159 12 80 359 61 364 2.26e-31 122
MS.gene53744.t1 MTR_8g094180 28.242 347 168 11 70 355 36 362 1.64e-29 117
MS.gene53744.t1 MTR_8g094180 29.492 295 150 8 70 326 36 310 4.56e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53744.t1 AT3G52060 54.268 328 129 4 43 362 32 346 2.78e-121 354
MS.gene53744.t1 AT5G22070 48.485 363 169 7 17 362 1 362 3.21e-116 342
MS.gene53744.t1 AT5G25330 47.811 297 125 7 81 362 81 362 1.03e-83 259
MS.gene53744.t1 AT4G32290 48.505 301 122 5 81 362 93 379 4.15e-78 245
MS.gene53744.t1 AT4G31350 36.455 299 144 7 80 352 88 366 1.73e-48 168
MS.gene53744.t1 AT4G31350 36.455 299 144 7 80 352 88 366 1.73e-48 168
MS.gene53744.t1 AT4G31350 36.455 299 144 7 80 352 88 366 1.73e-48 168
MS.gene53744.t1 AT4G31350 36.455 299 139 8 80 352 88 361 1.47e-47 165
MS.gene53744.t1 AT1G51770 33.626 342 190 10 33 359 71 390 6.80e-44 156
MS.gene53744.t1 AT2G19160 31.016 374 197 10 22 354 33 386 6.83e-44 156
MS.gene53744.t1 AT2G19160 31.016 374 197 10 22 354 33 386 6.83e-44 156
MS.gene53744.t1 AT5G25970 34.722 288 152 8 80 355 118 381 9.09e-43 153
MS.gene53744.t1 AT5G25970 34.722 288 152 8 80 355 118 381 9.09e-43 153
MS.gene53744.t1 AT5G25970 34.722 288 152 8 80 355 118 381 9.09e-43 153
MS.gene53744.t1 AT1G10280 36.082 291 153 8 80 359 142 410 1.61e-42 153
MS.gene53744.t1 AT3G21310 34.505 313 174 8 56 359 91 381 1.93e-42 152
MS.gene53744.t1 AT3G21310 34.505 313 174 8 56 359 91 381 1.93e-42 152
MS.gene53744.t1 AT4G30060 32.903 310 155 9 75 354 103 389 2.93e-42 152
MS.gene53744.t1 AT4G30060 32.903 310 155 9 75 354 103 389 2.93e-42 152
MS.gene53744.t1 AT5G11730 33.904 292 157 8 80 359 117 384 3.41e-42 151
MS.gene53744.t1 AT1G68380 35.254 295 151 9 80 360 123 391 3.05e-39 144
MS.gene53744.t1 AT5G57270 33.007 306 153 9 79 354 96 379 4.84e-39 143
MS.gene53744.t1 AT5G57270 33.007 306 153 9 79 354 96 379 4.84e-39 143
MS.gene53744.t1 AT5G57270 33.007 306 153 9 79 354 96 379 4.84e-39 143
MS.gene53744.t1 AT5G57270 33.007 306 153 9 79 354 96 379 4.84e-39 143
MS.gene53744.t1 AT5G57270 33.007 306 153 9 79 354 96 379 4.84e-39 143
MS.gene53744.t1 AT5G16170 34.237 295 155 8 81 359 136 407 1.73e-38 142
MS.gene53744.t1 AT1G68390 33.333 291 162 9 80 359 137 406 4.22e-37 138
MS.gene53744.t1 AT1G10880 34.146 287 155 9 80 355 117 380 5.11e-37 137
MS.gene53744.t1 AT4G25870 32.119 302 157 7 81 354 99 380 1.04e-36 137
MS.gene53744.t1 AT1G73810 35.216 301 152 11 76 359 142 416 7.89e-34 129
MS.gene53744.t1 AT1G51770 30.409 342 174 10 33 359 71 363 1.39e-31 123
MS.gene53744.t1 AT5G14550 29.371 286 158 7 76 341 60 321 3.38e-31 121
MS.gene53744.t1 AT5G14550 27.508 309 166 8 76 350 60 344 2.91e-28 114
MS.gene53744.t1 AT5G14550 27.508 309 166 8 76 350 60 344 3.21e-28 113
MS.gene53744.t1 AT1G11940 26.933 401 211 16 6 359 10 375 7.89e-28 112
MS.gene53744.t1 AT1G62305 29.283 321 159 11 79 353 67 365 1.81e-26 108
MS.gene53744.t1 AT1G62305 31.897 232 115 8 79 289 67 276 1.33e-23 100
MS.gene53744.t1 AT3G21310 41.379 145 81 4 56 199 91 232 7.48e-22 94.0
MS.gene53744.t1 AT1G11940 28.519 270 154 9 6 269 10 246 1.17e-21 93.6
MS.gene53744.t1 AT5G14550 26.804 291 164 9 76 350 60 317 3.23e-21 93.6

Find 92 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGACCTTCGCACCTTTA+TGG 0.111596 1.3:-30361227 MS.gene53744:CDS
GAGGAAGAGGAAAGCGATTT+TGG 0.152318 1.3:+30361260 None:intergenic
ACACAACCTTTGTATGTTTC+CGG 0.216341 1.3:+30360755 None:intergenic
TTACACTGACTAGAGTTAAT+TGG 0.219828 1.3:-30360609 MS.gene53744:CDS
CCTGACCTTCGCACCTTTAT+GGG 0.229629 1.3:-30361226 MS.gene53744:CDS
AATTGGACCGGTTCTGTCTA+TGG 0.305698 1.3:-30360592 MS.gene53744:CDS
AGAGCTAGATAGACAATAAT+TGG 0.321477 1.3:+30361438 None:intergenic
TTGAAGGGATGAAACGGTTT+TGG 0.330698 1.3:+30361115 None:intergenic
CCCATAAAGGTGCGAAGGTC+AGG 0.352360 1.3:+30361226 None:intergenic
GAAAATGTGGGTGGTGGTGT+GGG 0.369281 1.3:+30361302 None:intergenic
AGGGTGAGGTCGTTGGCGTC+GGG 0.370602 1.3:+30361342 None:intergenic
GGGTTGTGGCGCGGCGGAAG+AGG 0.385465 1.3:+30361322 None:intergenic
GGTGAGGTCGTTGGCGTCGG+GGG 0.387981 1.3:+30361344 None:intergenic
TGAAAATGTGGGTGGTGGTG+TGG 0.392713 1.3:+30361301 None:intergenic
CAATAATACTTATTTATACA+TGG 0.408546 1.3:+30360697 None:intergenic
GTGGGTGGTGGTGTGGGTTG+TGG 0.409947 1.3:+30361308 None:intergenic
GGTTAGAGTTGGTGAGGAAG+AGG 0.414237 1.3:+30361247 None:intergenic
AGGCAGAAAATATCAATATA+CGG 0.414838 1.3:-30360861 MS.gene53744:CDS
TCTATCAATGGAGGACTTGA+AGG 0.417198 1.3:-30360644 MS.gene53744:CDS
TAGATATGAATAACTCCAAT+TGG 0.419719 1.3:+30360507 None:intergenic
GAGGGTGAGGTCGTTGGCGT+CGG 0.423861 1.3:+30361341 None:intergenic
ACATTCCGCTGAGAATTTCC+TGG 0.434041 1.3:+30360480 None:intergenic
GTCAGCTGAGGGTGTCCAAT+TGG 0.435014 1.3:-30360522 MS.gene53744:CDS
TGGAGAGAATGTAATGTTGC+CGG 0.436873 1.3:-30360827 MS.gene53744:CDS
GTTGCCGGAGGTTCCGTTTG+AGG 0.437713 1.3:-30360812 MS.gene53744:CDS
TGAGAGAATCTCAATATAGC+TGG 0.445207 1.3:+30360885 None:intergenic
GGTGTTGTTGGGTGAGAAAG+AGG 0.450731 1.3:+30361394 None:intergenic
GTTGACAACAATGGATTTAG+TGG 0.454154 1.3:+30361462 None:intergenic
TGGTGGTGTGGGTTGTGGCG+CGG 0.456059 1.3:+30361313 None:intergenic
TGGGTGAGAAAGAGGATAAG+TGG 0.458255 1.3:+30361402 None:intergenic
GTCGGAAAATAATGTTCCTC+TGG 0.476768 1.3:+30360670 None:intergenic
GGGTGAGAAAGAGGATAAGT+GGG 0.482380 1.3:+30361403 None:intergenic
GGGAGATAAGTGCAAAGTAT+TGG 0.484120 1.3:+30361010 None:intergenic
AGCTCTGGCGACACCTCTGC+TGG 0.488365 1.3:+30360550 None:intergenic
GTTATTCATATCTATTCGCC+AGG 0.491313 1.3:-30360498 MS.gene53744:CDS
GGTTGTGGCGCGGCGGAAGA+GGG 0.493860 1.3:+30361323 None:intergenic
GAAGAGCGGAATACAATGTT+GGG 0.494292 1.3:+30360990 None:intergenic
ACCACCTATGTCGTCTCCCC+CGG 0.495251 1.3:-30361147 MS.gene53744:CDS
GATGGAGAGTAACGTTTGGT+GGG 0.499446 1.3:+30361090 None:intergenic
TGGCGCGGCGGAAGAGGGTG+AGG 0.501117 1.3:+30361328 None:intergenic
CGGGCAGCCGCGATGAGTGA+TGG 0.513763 1.3:+30361072 None:intergenic
GGTTCAGTGGGTCATCTAAG+AGG 0.514148 1.3:+30361031 None:intergenic
AGAAGAGCGGAATACAATGT+TGG 0.517351 1.3:+30360989 None:intergenic
GAGAGAATCTCAATATAGCT+GGG 0.521210 1.3:+30360886 None:intergenic
TGATGGAGAGTAACGTTTGG+TGG 0.531016 1.3:+30361089 None:intergenic
GCGGAAGAGGGTGAGGTCGT+TGG 0.536313 1.3:+30361335 None:intergenic
GGCAGATGCTAGGAGACGGC+GGG 0.537343 1.3:+30361053 None:intergenic
AAATCCTCAAACGGAACCTC+CGG 0.539492 1.3:+30360808 None:intergenic
GGGTGAGGTCGTTGGCGTCG+GGG 0.541309 1.3:+30361343 None:intergenic
TCCTCCATTGATAGAAGAGT+CGG 0.541364 1.3:+30360652 None:intergenic
ATAATTGAGAGAGGTGTTGT+TGG 0.544624 1.3:+30361382 None:intergenic
CAATATAGCTGGGGTACAGT+AGG 0.548185 1.3:+30360896 None:intergenic
GAGGGTGAAAATGTGGGTGG+TGG 0.551330 1.3:+30361296 None:intergenic
CATCTAAGAGGGCAGATGCT+AGG 0.551980 1.3:+30361043 None:intergenic
TGTTGAGAGATCAAAAGCTA+TGG 0.553117 1.3:-30360735 MS.gene53744:CDS
CCAACCCGAAAATCCTCAAA+CGG 0.556590 1.3:+30360799 None:intergenic
CTATCAATGGAGGACTTGAA+GGG 0.558282 1.3:-30360643 MS.gene53744:CDS
TGGATGGCCATAGACAGAAC+CGG 0.566191 1.3:+30360585 None:intergenic
TAATTGAGAGAGGTGTTGTT+GGG 0.570275 1.3:+30361383 None:intergenic
GTGCAAAGTATTGGTTCAGT+GGG 0.571044 1.3:+30361019 None:intergenic
TGCGAAGGTCAGGTTAGAGT+TGG 0.571465 1.3:+30361236 None:intergenic
CTGACTAGAGTTAATTGGAC+CGG 0.573741 1.3:-30360604 MS.gene53744:CDS
AAGGGAAGGATAATTGAGAG+AGG 0.583106 1.3:+30361373 None:intergenic
ATAATTGGTGTTGACAACAA+TGG 0.584186 1.3:+30361453 None:intergenic
TCCGACTCTTCTATCAATGG+AGG 0.586529 1.3:-30360653 MS.gene53744:CDS
AGTGCAAAGTATTGGTTCAG+TGG 0.589016 1.3:+30361018 None:intergenic
AATATACGGTATAATGCTCG+TGG 0.592590 1.3:-30360847 MS.gene53744:CDS
GTACAGTAGGTTAAATTCAG+AGG 0.594694 1.3:+30360909 None:intergenic
GTTCAGTGGGTCATCTAAGA+GGG 0.599121 1.3:+30361032 None:intergenic
GGGCAGATGCTAGGAGACGG+CGG 0.605522 1.3:+30361052 None:intergenic
AGGTCAGGTTAGAGTTGGTG+AGG 0.605637 1.3:+30361241 None:intergenic
TGGTGTGGGTTGTGGCGCGG+CGG 0.613974 1.3:+30361316 None:intergenic
CATAGGTGGTGGGATCTGCA+TGG 0.615109 1.3:+30361160 None:intergenic
TATGAGGGTGAAAATGTGGG+TGG 0.616892 1.3:+30361293 None:intergenic
TAAGTATTATTGTTATCCAG+AGG 0.629370 1.3:-30360686 MS.gene53744:CDS
AGAGGGCAGATGCTAGGAGA+CGG 0.639417 1.3:+30361049 None:intergenic
CTTGAACCGGAAACATACAA+AGG 0.648378 1.3:-30360761 MS.gene53744:CDS
CTATATTGAGATTCTCTCAG+AGG 0.650945 1.3:-30360881 MS.gene53744:CDS
ATGAATTGAAACGAGAAGAG+CGG 0.654333 1.3:+30360976 None:intergenic
TATCAATGGAGGACTTGAAG+GGG 0.655133 1.3:-30360642 MS.gene53744:CDS
TCATCAGCAATATTCATCAA+CGG 0.655931 1.3:+30360451 None:intergenic
GAGTGATGGAGAGTAACGTT+TGG 0.656827 1.3:+30361086 None:intergenic
TCTGCTGGAGTATAAAGATG+TGG 0.660732 1.3:+30360565 None:intergenic
GGACGTAGATGTTGAAGAGG+TGG 0.662229 1.3:+30361181 None:intergenic
AGAGAATCTCAATATAGCTG+GGG 0.663742 1.3:+30360887 None:intergenic
CTGGAGTATAAAGATGTGGA+TGG 0.667016 1.3:+30360569 None:intergenic
GGGGAGACGACATAGGTGGT+GGG 0.667875 1.3:+30361150 None:intergenic
CATGGACGTAGATGTTGAAG+AGG 0.678016 1.3:+30361178 None:intergenic
ACATCTTTATACTCCAGCAG+AGG 0.685951 1.3:-30360563 MS.gene53744:CDS
AGAGAATGTAATGTTGCCGG+AGG 0.692280 1.3:-30360824 MS.gene53744:CDS
ATTCGCCAGGAAATTCTCAG+CGG 0.766399 1.3:-30360485 MS.gene53744:CDS
TTACTCTCCATCACTCATCG+CGG 0.773471 1.3:-30361079 MS.gene53744:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTAATTGATTTTTAAAA+AGG + chr1.3:30360989-30361008 None:intergenic 10.0%
!! CAATAATACTTATTTATACA+TGG + chr1.3:30361236-30361255 None:intergenic 15.0%
!!! ATTTAGTGGTTTTTTATTGA+GGG + chr1.3:30360457-30360476 None:intergenic 20.0%
! AGGCAGAAAATATCAATATA+CGG - chr1.3:30361069-30361088 MS.gene53744:CDS 25.0%
! TAAGTATTATTGTTATCCAG+AGG - chr1.3:30361244-30361263 MS.gene53744:CDS 25.0%
! TAGATATGAATAACTCCAAT+TGG + chr1.3:30361426-30361445 None:intergenic 25.0%
!! ACAAAGAATTTTTCCCATAA+AGG + chr1.3:30360720-30360739 None:intergenic 25.0%
!!! CACAATTTTTTATCTTGAAC+CGG - chr1.3:30361156-30361175 MS.gene53744:CDS 25.0%
!!! GATTTAGTGGTTTTTTATTG+AGG + chr1.3:30360458-30360477 None:intergenic 25.0%
!!! TTTAGTGGTTTTTTATTGAG+GGG + chr1.3:30360456-30360475 None:intergenic 25.0%
AGAGCTAGATAGACAATAAT+TGG + chr1.3:30360495-30360514 None:intergenic 30.0%
TCATCAGCAATATTCATCAA+CGG + chr1.3:30361482-30361501 None:intergenic 30.0%
! TTACACTGACTAGAGTTAAT+TGG - chr1.3:30361321-30361340 MS.gene53744:CDS 30.0%
! TTTTATGAGGGTGAAAATGT+GGG + chr1.3:30360643-30360662 None:intergenic 30.0%
!! ATAATTGGTGTTGACAACAA+TGG + chr1.3:30360480-30360499 None:intergenic 30.0%
!!! GTTTTTTATTGAGGGGTTTT+GGG + chr1.3:30360449-30360468 None:intergenic 30.0%
!!! TTTTTTATTGAGGGGTTTTG+GGG + chr1.3:30360448-30360467 None:intergenic 30.0%
AATATACGGTATAATGCTCG+TGG - chr1.3:30361083-30361102 MS.gene53744:CDS 35.0%
ACACAACCTTTGTATGTTTC+CGG + chr1.3:30361178-30361197 None:intergenic 35.0%
AGAGAATCTCAATATAGCTG+GGG + chr1.3:30361046-30361065 None:intergenic 35.0%
ATGAATTGAAACGAGAAGAG+CGG + chr1.3:30360957-30360976 None:intergenic 35.0%
CTATATTGAGATTCTCTCAG+AGG - chr1.3:30361049-30361068 MS.gene53744:CDS 35.0%
GAGAGAATCTCAATATAGCT+GGG + chr1.3:30361047-30361066 None:intergenic 35.0%
GTACAGTAGGTTAAATTCAG+AGG + chr1.3:30361024-30361043 None:intergenic 35.0%
GTTATTCATATCTATTCGCC+AGG - chr1.3:30361432-30361451 MS.gene53744:CDS 35.0%
TGAGAGAATCTCAATATAGC+TGG + chr1.3:30361048-30361067 None:intergenic 35.0%
TGTTGAGAGATCAAAAGCTA+TGG - chr1.3:30361195-30361214 MS.gene53744:CDS 35.0%
! GTTGACAACAATGGATTTAG+TGG + chr1.3:30360471-30360490 None:intergenic 35.0%
! GTTTTATGAGGGTGAAAATG+TGG + chr1.3:30360644-30360663 None:intergenic 35.0%
! TTTTCCGACTCTTCTATCAA+TGG - chr1.3:30361274-30361293 MS.gene53744:CDS 35.0%
!! ATAATTGAGAGAGGTGTTGT+TGG + chr1.3:30360551-30360570 None:intergenic 35.0%
!! TAATTGAGAGAGGTGTTGTT+GGG + chr1.3:30360550-30360569 None:intergenic 35.0%
!! TTTGGGTTTGGGTTTTATGA+GGG + chr1.3:30360655-30360674 None:intergenic 35.0%
!!! GGTTTTTTATTGAGGGGTTT+TGG + chr1.3:30360450-30360469 None:intergenic 35.0%
!!! TTTTGGGTTTGGGTTTTATG+AGG + chr1.3:30360656-30360675 None:intergenic 35.0%
AAGGGAAGGATAATTGAGAG+AGG + chr1.3:30360560-30360579 None:intergenic 40.0%
ACATCTTTATACTCCAGCAG+AGG - chr1.3:30361367-30361386 MS.gene53744:CDS 40.0%
AGAAGAGCGGAATACAATGT+TGG + chr1.3:30360944-30360963 None:intergenic 40.0%
CTATCAATGGAGGACTTGAA+GGG - chr1.3:30361287-30361306 MS.gene53744:CDS 40.0%
CTGACTAGAGTTAATTGGAC+CGG - chr1.3:30361326-30361345 MS.gene53744:CDS 40.0%
CTGGAGTATAAAGATGTGGA+TGG + chr1.3:30361364-30361383 None:intergenic 40.0%
CTTGAACCGGAAACATACAA+AGG - chr1.3:30361169-30361188 MS.gene53744:CDS 40.0%
GAAGAGCGGAATACAATGTT+GGG + chr1.3:30360943-30360962 None:intergenic 40.0%
GGGAGATAAGTGCAAAGTAT+TGG + chr1.3:30360923-30360942 None:intergenic 40.0%
GTCGGAAAATAATGTTCCTC+TGG + chr1.3:30361263-30361282 None:intergenic 40.0%
TATCAATGGAGGACTTGAAG+GGG - chr1.3:30361288-30361307 MS.gene53744:CDS 40.0%
TCCTCCATTGATAGAAGAGT+CGG + chr1.3:30361281-30361300 None:intergenic 40.0%
TCTATCAATGGAGGACTTGA+AGG - chr1.3:30361286-30361305 MS.gene53744:CDS 40.0%
TCTGCTGGAGTATAAAGATG+TGG + chr1.3:30361368-30361387 None:intergenic 40.0%
TGGAGAGAATGTAATGTTGC+CGG - chr1.3:30361103-30361122 MS.gene53744:CDS 40.0%
! AGGAAGAGGAAAGCGATTTT+GGG + chr1.3:30360672-30360691 None:intergenic 40.0%
! AGGTTCCGTTTGAGGATTTT+CGG - chr1.3:30361126-30361145 MS.gene53744:CDS 40.0%
! TTGAAGGGATGAAACGGTTT+TGG + chr1.3:30360818-30360837 None:intergenic 40.0%
!! AGTGCAAAGTATTGGTTCAG+TGG + chr1.3:30360915-30360934 None:intergenic 40.0%
!! GTGCAAAGTATTGGTTCAGT+GGG + chr1.3:30360914-30360933 None:intergenic 40.0%
!! TTTTTCCCATAAAGGTGCGA+AGG + chr1.3:30360712-30360731 None:intergenic 40.0%
!!! AACGTTTGGTGGGTTTTGAA+GGG + chr1.3:30360833-30360852 None:intergenic 40.0%
!!! AGGAAAGCGATTTTGGGTTT+GGG + chr1.3:30360666-30360685 None:intergenic 40.0%
!!! TAACGTTTGGTGGGTTTTGA+AGG + chr1.3:30360834-30360853 None:intergenic 40.0%
!!! TGCTGATGACGTCATTTTTC+AGG - chr1.3:30361493-30361512 MS.gene53744:CDS 40.0%
!!! TGGGTTTTGAAGGGATGAAA+CGG + chr1.3:30360824-30360843 None:intergenic 40.0%
AAATCCTCAAACGGAACCTC+CGG + chr1.3:30361125-30361144 None:intergenic 45.0%
ACATTCCGCTGAGAATTTCC+TGG + chr1.3:30361453-30361472 None:intergenic 45.0%
AGAGAATGTAATGTTGCCGG+AGG - chr1.3:30361106-30361125 MS.gene53744:CDS 45.0%
ATTCGCCAGGAAATTCTCAG+CGG - chr1.3:30361445-30361464 MS.gene53744:CDS 45.0%
CAATATAGCTGGGGTACAGT+AGG + chr1.3:30361037-30361056 None:intergenic 45.0%
CATGGACGTAGATGTTGAAG+AGG + chr1.3:30360755-30360774 None:intergenic 45.0%
CCAACCCGAAAATCCTCAAA+CGG + chr1.3:30361134-30361153 None:intergenic 45.0%
GATGGAGAGTAACGTTTGGT+GGG + chr1.3:30360843-30360862 None:intergenic 45.0%
GGGTGAGAAAGAGGATAAGT+GGG + chr1.3:30360530-30360549 None:intergenic 45.0%
GTTCAGTGGGTCATCTAAGA+GGG + chr1.3:30360901-30360920 None:intergenic 45.0%
TATGAGGGTGAAAATGTGGG+TGG + chr1.3:30360640-30360659 None:intergenic 45.0%
TCCGACTCTTCTATCAATGG+AGG - chr1.3:30361277-30361296 MS.gene53744:CDS 45.0%
TGATGGAGAGTAACGTTTGG+TGG + chr1.3:30360844-30360863 None:intergenic 45.0%
TGGGTGAGAAAGAGGATAAG+TGG + chr1.3:30360531-30360550 None:intergenic 45.0%
TTACTCTCCATCACTCATCG+CGG - chr1.3:30360851-30360870 MS.gene53744:CDS 45.0%
! AATTGGACCGGTTCTGTCTA+TGG - chr1.3:30361338-30361357 MS.gene53744:CDS 45.0%
! GAGGAAGAGGAAAGCGATTT+TGG + chr1.3:30360673-30360692 None:intergenic 45.0%
! GAGTGATGGAGAGTAACGTT+TGG + chr1.3:30360847-30360866 None:intergenic 45.0%
! GGTTCCGTTTGAGGATTTTC+GGG - chr1.3:30361127-30361146 MS.gene53744:CDS 45.0%
!!! AGAGCTTTTTCGTCAGCTGA+GGG - chr1.3:30361397-30361416 MS.gene53744:CDS 45.0%
!!! GAGGAAAGCGATTTTGGGTT+TGG + chr1.3:30360667-30360686 None:intergenic 45.0%
ACCTGACCTTCGCACCTTTA+TGG - chr1.3:30360703-30360722 MS.gene53744:CDS 50.0%
CATCTAAGAGGGCAGATGCT+AGG + chr1.3:30360890-30360909 None:intergenic 50.0%
CCTGACCTTCGCACCTTTAT+GGG - chr1.3:30360704-30360723 MS.gene53744:CDS 50.0%
CTCAGCTGACGAAAAAGCTC+TGG + chr1.3:30361398-30361417 None:intergenic 50.0%
GGACGTAGATGTTGAAGAGG+TGG + chr1.3:30360752-30360771 None:intergenic 50.0%
GGTTCAGTGGGTCATCTAAG+AGG + chr1.3:30360902-30360921 None:intergenic 50.0%
TGCGAAGGTCAGGTTAGAGT+TGG + chr1.3:30360697-30360716 None:intergenic 50.0%
TGGATGGCCATAGACAGAAC+CGG + chr1.3:30361348-30361367 None:intergenic 50.0%
! GAAAATGTGGGTGGTGGTGT+GGG + chr1.3:30360631-30360650 None:intergenic 50.0%
! TGAAAATGTGGGTGGTGGTG+TGG + chr1.3:30360632-30360651 None:intergenic 50.0%
!! AGGTCAGGTTAGAGTTGGTG+AGG + chr1.3:30360692-30360711 None:intergenic 50.0%
!! GGTGTTGTTGGGTGAGAAAG+AGG + chr1.3:30360539-30360558 None:intergenic 50.0%
!! GGTTAGAGTTGGTGAGGAAG+AGG + chr1.3:30360686-30360705 None:intergenic 50.0%
!!! ACGGTTTTGGAAGACACCAC+CGG + chr1.3:30360805-30360824 None:intergenic 50.0%
!!! CAGAGCTTTTTCGTCAGCTG+AGG - chr1.3:30361396-30361415 MS.gene53744:CDS 50.0%
!!! CCGTTTGAGGATTTTCGGGT+TGG - chr1.3:30361131-30361150 MS.gene53744:CDS 50.0%
AGAGGGCAGATGCTAGGAGA+CGG + chr1.3:30360884-30360903 None:intergenic 55.0%
GAGGGTGAAAATGTGGGTGG+TGG + chr1.3:30360637-30360656 None:intergenic 55.0%
! CCCATAAAGGTGCGAAGGTC+AGG + chr1.3:30360707-30360726 None:intergenic 55.0%
!! CATAGGTGGTGGGATCTGCA+TGG + chr1.3:30360773-30360792 None:intergenic 55.0%
!! GTCAGCTGAGGGTGTCCAAT+TGG - chr1.3:30361408-30361427 MS.gene53744:CDS 55.0%
!!! CGGTTTTGGAAGACACCACC+GGG + chr1.3:30360804-30360823 None:intergenic 55.0%
!!! GGTTTTGGAAGACACCACCG+GGG + chr1.3:30360803-30360822 None:intergenic 55.0%
!!! GTTTTGGAAGACACCACCGG+GGG + chr1.3:30360802-30360821 None:intergenic 55.0%
ACCACCTATGTCGTCTCCCC+CGG - chr1.3:30360783-30360802 MS.gene53744:CDS 60.0%
GGGGAGACGACATAGGTGGT+GGG + chr1.3:30360783-30360802 None:intergenic 60.0%
GTTGCCGGAGGTTCCGTTTG+AGG - chr1.3:30361118-30361137 MS.gene53744:CDS 60.0%
ACCACCGGGGGAGACGACAT+AGG + chr1.3:30360790-30360809 None:intergenic 65.0%
ACCGGGGGAGACGACATAGG+TGG + chr1.3:30360787-30360806 None:intergenic 65.0%
ACCTATGTCGTCTCCCCCGG+TGG - chr1.3:30360786-30360805 MS.gene53744:CDS 65.0%
AGGGTGAGGTCGTTGGCGTC+GGG + chr1.3:30360591-30360610 None:intergenic 65.0%
GAGGGTGAGGTCGTTGGCGT+CGG + chr1.3:30360592-30360611 None:intergenic 65.0%
GCGGAAGAGGGTGAGGTCGT+TGG + chr1.3:30360598-30360617 None:intergenic 65.0%
GGCAGATGCTAGGAGACGGC+GGG + chr1.3:30360880-30360899 None:intergenic 65.0%
GGGCAGATGCTAGGAGACGG+CGG + chr1.3:30360881-30360900 None:intergenic 65.0%
GGGGGAGACGACATAGGTGG+TGG + chr1.3:30360784-30360803 None:intergenic 65.0%
GTCGGGGGAGGAGAAAGGGA+AGG + chr1.3:30360574-30360593 None:intergenic 65.0%
! AGCTCTGGCGACACCTCTGC+TGG + chr1.3:30361383-30361402 None:intergenic 65.0%
! GTGGGTGGTGGTGTGGGTTG+TGG + chr1.3:30360625-30360644 None:intergenic 65.0%
! TGGCGTCGGGGGAGGAGAAA+GGG + chr1.3:30360578-30360597 None:intergenic 65.0%
! TGGTGGTGTGGGTTGTGGCG+CGG + chr1.3:30360620-30360639 None:intergenic 65.0%
! TTGGCGTCGGGGGAGGAGAA+AGG + chr1.3:30360579-30360598 None:intergenic 65.0%
CGGGCAGCCGCGATGAGTGA+TGG + chr1.3:30360861-30360880 None:intergenic 70.0%
GGGTGAGGTCGTTGGCGTCG+GGG + chr1.3:30360590-30360609 None:intergenic 70.0%
GGTTGTGGCGCGGCGGAAGA+GGG + chr1.3:30360610-30360629 None:intergenic 70.0%
! GGTGAGGTCGTTGGCGTCGG+GGG + chr1.3:30360589-30360608 None:intergenic 70.0%
! TGGTGTGGGTTGTGGCGCGG+CGG + chr1.3:30360617-30360636 None:intergenic 70.0%
GGGTTGTGGCGCGGCGGAAG+AGG + chr1.3:30360611-30360630 None:intergenic 75.0%
TGGCGCGGCGGAAGAGGGTG+AGG + chr1.3:30360605-30360624 None:intergenic 75.0%
! GAGGTCGTTGGCGTCGGGGG+AGG + chr1.3:30360586-30360605 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 30360432 30361520 30360432 ID=MS.gene53744
chr1.3 mRNA 30360432 30361520 30360432 ID=MS.gene53744.t1;Parent=MS.gene53744
chr1.3 exon 30360432 30361520 30360432 ID=MS.gene53744.t1.exon1;Parent=MS.gene53744.t1
chr1.3 CDS 30360432 30361520 30360432 ID=cds.MS.gene53744.t1;Parent=MS.gene53744.t1
Gene Sequence

>MS.gene53744

ATGGCAAAATTAACCCCAAAACCCCTCAATAAAAAACCACTAAATCCATTGTTGTCAACACCAATTATTGTCTATCTAGCTCTCATCATCTCTCTCCCACTTATCCTCTTTCTCACCCAACAACACCTCTCTCAATTATCCTTCCCTTTCTCCTCCCCCGACGCCAACGACCTCACCCTCTTCCGCCGCGCCACAACCCACACCACCACCCACATTTTCACCCTCATAAAACCCAAACCCAAAATCGCTTTCCTCTTCCTCACCAACTCTAACCTGACCTTCGCACCTTTATGGGAAAAATTCTTTGTTAGAAACAACCACCTCTTCAACATCTACGTCCATGCAGATCCCACCACCTATGTCGTCTCCCCCGGTGGTGTCTTCCAAAACCGTTTCATCCCTTCAAAACCCACCAAACGTTACTCTCCATCACTCATCGCGGCTGCCCGCCGTCTCCTAGCATCTGCCCTCTTAGATGACCCACTGAACCAATACTTTGCACTTATCTCCCAACATTGTATTCCGCTCTTCTCGTTTCAATTCATTTATAACTACCTTTTTAAAAATCAATTAAAGTCATTTACAAATTCCTCTGAATTTAACCTACTGTACCCCAGCTATATTGAGATTCTCTCAGAGGCAGAAAATATCAATATACGGTATAATGCTCGTGGAGAGAATGTAATGTTGCCGGAGGTTCCGTTTGAGGATTTTCGGGTTGGTTCACAATTTTTTATCTTGAACCGGAAACATACAAAGGTTGTGTTGAGAGATCAAAAGCTATGGAACAAGTTTCAGATTCCATGTATAAATAAGTATTATTGTTATCCAGAGGAACATTATTTTCCGACTCTTCTATCAATGGAGGACTTGAAGGGGTGTACTTGTTTTACACTGACTAGAGTTAATTGGACCGGTTCTGTCTATGGCCATCCACATCTTTATACTCCAGCAGAGGTGTCGCCAGAGCTTTTTCGTCAGCTGAGGGTGTCCAATTGGAGTTATTCATATCTATTCGCCAGGAAATTCTCAGCGGAATGTCTTGCTCCGTTGATGAATATTGCTGATGACGTCATTTTTCAGGATTGA
Protein sequence

>MS.gene53744.t1

MAKLTPKPLNKKPLNPLLSTPIIVYLALIISLPLILFLTQQHLSQLSFPFSSPDANDLTLFRRATTHTTTHIFTLIKPKPKIAFLFLTNSNLTFAPLWEKFFVRNNHLFNIYVHADPTTYVVSPGGVFQNRFIPSKPTKRYSPSLIAAARRLLASALLDDPLNQYFALISQHCIPLFSFQFIYNYLFKNQLKSFTNSSEFNLLYPSYIEILSEAENINIRYNARGENVMLPEVPFEDFRVGSQFFILNRKHTKVVLRDQKLWNKFQIPCINKYYCYPEEHYFPTLLSMEDLKGCTCFTLTRVNWTGSVYGHPHLYTPAEVSPELFRQLRVSNWSYSYLFARKFSAECLAPLMNIADDVIFQD