AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54312

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54312.t1 MTR_2g015890 94.922 256 13 0 1 256 1 256 0.0 499
MS.gene54312.t1 MTR_2g015890 81.250 256 8 1 1 256 1 216 3.07e-146 407
MS.gene54312.t1 MTR_2g015890 92.405 158 12 0 99 256 1 158 1.51e-105 302
MS.gene54312.t1 MTR_4g087920 55.932 236 99 3 3 233 10 245 2.14e-71 220
MS.gene54312.t1 MTR_2g015890 70.253 158 7 1 99 256 1 118 7.67e-70 211
MS.gene54312.t1 MTR_4g079760 47.727 220 109 4 19 234 21 238 1.08e-53 174
MS.gene54312.t1 MTR_2g091190 49.268 205 98 3 29 229 23 225 1.19e-52 171
MS.gene54312.t1 MTR_3g070970 38.506 174 88 4 67 227 73 240 2.70e-28 108
MS.gene54312.t1 MTR_1g093750 48.201 139 65 1 63 194 154 292 2.80e-28 110
MS.gene54312.t1 MTR_5g032375 34.694 147 82 3 56 189 34 179 1.06e-17 79.3
MS.gene54312.t1 MTR_5g066080 32.883 222 118 4 63 254 33 253 2.79e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54312.t1 AT3G56770 40.116 172 92 5 62 228 40 205 7.62e-31 114
MS.gene54312.t1 AT2G40200 38.889 162 91 2 69 224 65 224 3.39e-30 113
MS.gene54312.t1 AT1G68810 43.605 172 91 4 56 223 164 333 1.88e-28 111
MS.gene54312.t1 AT2G41130 34.529 223 130 5 17 228 11 228 4.94e-27 105
MS.gene54312.t1 AT3G25710 44.118 136 73 2 56 189 122 256 1.64e-24 100
MS.gene54312.t1 AT4G38070 34.959 123 76 2 66 188 91 209 4.17e-17 78.6

Find 46 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAACAATGTCAACTTAT+TGG 0.161618 2.3:+69493270 MS.gene54312:CDS
CTTTACTAGCTGAGGTTATT+AGG 0.226217 2.3:+69492971 MS.gene54312:CDS
ATCAACAAACTCATTGGTTT+TGG 0.255474 2.3:+69491310 MS.gene54312:CDS
CTTAGATCAGTTAGAAGTTC+TGG 0.280659 2.3:-69493135 None:intergenic
AAGTCCCCTCCAATAATTAA+TGG 0.304566 2.3:+69491356 MS.gene54312:CDS
AATGCCACTTCATATAGATT+TGG 0.335342 2.3:+69491254 MS.gene54312:CDS
TAGTGGCTCTGAAGAATCAT+AGG 0.340919 2.3:+69491411 MS.gene54312:CDS
TGATGCTAACAAAACTTTAG+TGG 0.343142 2.3:+69491394 MS.gene54312:CDS
AAGAGAAGAAACAGAATTAA+TGG 0.350077 2.3:+69491446 MS.gene54312:CDS
TGATAAACTTCAACTACAAT+TGG 0.375139 2.3:+69493167 MS.gene54312:CDS
CCAGTACAGAACTCAGTTCC+TGG 0.377817 2.3:-69493305 None:intergenic
AAAGTTGAACCATGTGAAAA+TGG 0.387199 2.3:+69493069 MS.gene54312:CDS
AAGGGAAGAAATATCAACTT+TGG 0.396844 2.3:+69493191 MS.gene54312:CDS
AAATATCAACTTTGGGAGAC+AGG 0.404598 2.3:+69493199 MS.gene54312:CDS
TTCACTTCATCATCATCTGT+TGG 0.414183 2.3:-69493048 None:intergenic
GTTTGTATCAACAAACTCAT+TGG 0.426459 2.3:+69491304 MS.gene54312:CDS
TTCTCTCCATTAATTATTGG+AGG 0.433494 2.3:-69491362 None:intergenic
AGGGAAGAAATATCAACTTT+GGG 0.445016 2.3:+69493192 MS.gene54312:CDS
AACTTCAACTACAATTGGTA+AGG 0.473092 2.3:+69493172 MS.gene54312:CDS
CCAGGAACTGAGTTCTGTAC+TGG 0.489161 2.3:+69493305 MS.gene54312:CDS
ACTGAGTTCTGTACTGGAAA+GGG 0.489265 2.3:+69493311 MS.gene54312:CDS
CTCTCCATTAATTATTGGAG+GGG 0.498785 2.3:-69491360 None:intergenic
TATTATACCTTTGGAGAGGA+GGG 0.509382 2.3:-69491497 None:intergenic
ATTATAGCTCTGATATTTCG+AGG 0.509996 2.3:+69491222 MS.gene54312:CDS
CAAATGAGACTTCAAGAGGT+GGG 0.540083 2.3:+69491282 MS.gene54312:CDS
TCTCTCCATTAATTATTGGA+GGG 0.544195 2.3:-69491361 None:intergenic
TTATTATACCTTTGGAGAGG+AGG 0.544800 2.3:-69491498 None:intergenic
AACTGAGTTCTGTACTGGAA+AGG 0.553572 2.3:+69493310 MS.gene54312:CDS
CAAAGCAACTTTACTAGCTG+AGG 0.560860 2.3:+69492963 MS.gene54312:CDS
AGTGGCTCTGAAGAATCATA+GGG 0.564496 2.3:+69491412 MS.gene54312:CDS
AATGCAGATGAAGCAAGTAA+AGG 0.565648 2.3:+69493015 MS.gene54312:CDS
ACTTGTTCCCTCCTCTCCAA+AGG 0.568287 2.3:+69491490 MS.gene54312:CDS
GGAAAGGGTTTCTACCTCGA+TGG 0.569167 2.3:+69493326 MS.gene54312:CDS
AGCACCAAATCTATATGAAG+TGG 0.573695 2.3:-69491258 None:intergenic
AATATCAACTTTGGGAGACA+GGG 0.583527 2.3:+69493200 MS.gene54312:CDS
TATTGGAAATAATGTTCACC+AGG 0.592874 2.3:+69493287 MS.gene54312:CDS
TCAAATGAGACTTCAAGAGG+TGG 0.607431 2.3:+69491281 MS.gene54312:CDS
AGATTATTATACCTTTGGAG+AGG 0.615783 2.3:-69491501 None:intergenic
ACTTCAACTACAATTGGTAA+GGG 0.620942 2.3:+69493173 MS.gene54312:CDS
ATCAACAAGAGCAAAACTCA+TGG 0.629280 2.3:-69493413 None:intergenic
GTTGAACCATGTGAAAATGG+TGG 0.637388 2.3:+69493072 MS.gene54312:CDS
TGCATCTGTTTGCAAAGACG+TGG 0.639661 2.3:-69493366 None:intergenic
GAGAGGAGGGAACAAGTGCA+CGG 0.657365 2.3:-69491484 None:intergenic
GCTTCAAATGAGACTTCAAG+AGG 0.660687 2.3:+69491278 MS.gene54312:CDS
AGAATCATAGGGAAGCAGAG+AGG 0.725124 2.3:+69491423 MS.gene54312:CDS
TCTTAACGAGTAATCCATCG+AGG 0.765808 2.3:-69493340 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTAAGGATTATTAAA+TGG - chr2.3:69492701-69492720 None:intergenic 10.0%
!! TATAAAAAATACGAAAAGAA+AGG - chr2.3:69491528-69491547 None:intergenic 15.0%
!!! ACTTCGTTATATTTTTTTAA+GGG + chr2.3:69491577-69491596 MS.gene54312:intron 15.0%
!!! ATTGTTATCTGATTTTAAAA+AGG + chr2.3:69491735-69491754 MS.gene54312:intron 15.0%
!!! TAGTATTAATTTATTTGTTG+TGG + chr2.3:69492619-69492638 MS.gene54312:intron 15.0%
!!! TTATAATGTTTAACTTTTGT+TGG - chr2.3:69492251-69492270 None:intergenic 15.0%
!!! TTGTTTTTAAAATATCTTTG+TGG + chr2.3:69491620-69491639 MS.gene54312:intron 15.0%
!! AAACTGTAAAAAAGTTCAAT+TGG - chr2.3:69491689-69491708 None:intergenic 20.0%
!! ACTATAATGTAACATCATAT+TGG + chr2.3:69492364-69492383 MS.gene54312:intron 20.0%
!! CTGAAAAAATACATTTACTT+AGG + chr2.3:69491931-69491950 MS.gene54312:intron 20.0%
!! TATGTACATACTGTTATTAA+TGG + chr2.3:69492914-69492933 MS.gene54312:intron 20.0%
!! TTTAAGGATTATTAAATGGT+TGG - chr2.3:69492697-69492716 None:intergenic 20.0%
!!! ATTAATTTATTTGTTGTGGT+GGG + chr2.3:69492623-69492642 MS.gene54312:intron 20.0%
!!! CACTTCGTTATATTTTTTTA+AGG + chr2.3:69491576-69491595 MS.gene54312:intron 20.0%
!!! TATTAATTTATTTGTTGTGG+TGG + chr2.3:69492622-69492641 MS.gene54312:intron 20.0%
!!! TTTTTCTCTCCATTAATTAT+TGG - chr2.3:69491368-69491387 None:intergenic 20.0%
! AAATGAAACAACCTTAGTTT+GGG - chr2.3:69492427-69492446 None:intergenic 25.0%
! AACATATGATTCTTAGTTGT+GGG + chr2.3:69492473-69492492 MS.gene54312:intron 25.0%
! AAGAGAAGAAACAGAATTAA+TGG + chr2.3:69491446-69491465 MS.gene54312:CDS 25.0%
! AAGGAAGATTATTATACCTT+TGG - chr2.3:69491509-69491528 None:intergenic 25.0%
! ATTCCTAATTGCAACTTAAT+TGG - chr2.3:69492126-69492145 None:intergenic 25.0%
! ATTGAACAATGTCAACTTAT+TGG + chr2.3:69493270-69493289 MS.gene54312:CDS 25.0%
! CTGATTTCTAATATGTGTTA+GGG + chr2.3:69492777-69492796 MS.gene54312:intron 25.0%
! TAAATGAAACAACCTTAGTT+TGG - chr2.3:69492428-69492447 None:intergenic 25.0%
! TAACATATGATTCTTAGTTG+TGG + chr2.3:69492472-69492491 MS.gene54312:intron 25.0%
! TATGTGTTAGGGTTTAATAT+CGG + chr2.3:69492788-69492807 MS.gene54312:intron 25.0%
! TCTGATTTCTAATATGTGTT+AGG + chr2.3:69492776-69492795 MS.gene54312:intron 25.0%
! TGATAAACTTCAACTACAAT+TGG + chr2.3:69493167-69493186 MS.gene54312:CDS 25.0%
AAAGTTGAACCATGTGAAAA+TGG + chr2.3:69493069-69493088 MS.gene54312:CDS 30.0%
AACTTCAACTACAATTGGTA+AGG + chr2.3:69493172-69493191 MS.gene54312:CDS 30.0%
AAGGGAAGAAATATCAACTT+TGG + chr2.3:69493191-69493210 MS.gene54312:CDS 30.0%
AAGTCAACTATATAAGGTTG+TGG + chr2.3:69492317-69492336 MS.gene54312:intron 30.0%
AAGTTCAATTGGTAAACCAT+CGG - chr2.3:69491678-69491697 None:intergenic 30.0%
ACACAATGATCGAATATGAT+TGG - chr2.3:69491556-69491575 None:intergenic 30.0%
ACTTCAACTACAATTGGTAA+GGG + chr2.3:69493173-69493192 MS.gene54312:CDS 30.0%
AGGGAAGAAATATCAACTTT+GGG + chr2.3:69493192-69493211 MS.gene54312:CDS 30.0%
ATTGAAATTCATGCAGCATT+GGG - chr2.3:69492043-69492062 None:intergenic 30.0%
ATTGATGGAAATACAAGCTA+TGG + chr2.3:69491988-69492007 MS.gene54312:intron 30.0%
GTTTGTATCAACAAACTCAT+TGG + chr2.3:69491304-69491323 MS.gene54312:CDS 30.0%
TATTGGAAATAATGTTCACC+AGG + chr2.3:69493287-69493306 MS.gene54312:CDS 30.0%
TCATTCAAAACGTAAGTCAT+AGG - chr2.3:69492748-69492767 None:intergenic 30.0%
TCTCTCCATTAATTATTGGA+GGG - chr2.3:69491364-69491383 None:intergenic 30.0%
TGATGCTAACAAAACTTTAG+TGG + chr2.3:69491394-69491413 MS.gene54312:CDS 30.0%
TTCTCTCCATTAATTATTGG+AGG - chr2.3:69491365-69491384 None:intergenic 30.0%
TTGATGGAAATACAAGCTAT+GGG + chr2.3:69491989-69492008 MS.gene54312:intron 30.0%
! AATGCCACTTCATATAGATT+TGG + chr2.3:69491254-69491273 MS.gene54312:CDS 30.0%
! ACACCAATTAAGTTGCAATT+AGG + chr2.3:69492120-69492139 MS.gene54312:intron 30.0%
! AGATTATTATACCTTTGGAG+AGG - chr2.3:69491504-69491523 None:intergenic 30.0%
! ATAAACATGTTTTACACCGA+TGG + chr2.3:69491659-69491678 MS.gene54312:intron 30.0%
! CCTTATATAGTTGACTTTAG+CGG - chr2.3:69492314-69492333 None:intergenic 30.0%
! TTGACTGGTCAAATGTATAT+GGG + chr2.3:69492286-69492305 MS.gene54312:intron 30.0%
! TTTTGCAGATTTATCTTCGA+TGG + chr2.3:69492400-69492419 MS.gene54312:intron 30.0%
!! ATTATAGCTCTGATATTTCG+AGG + chr2.3:69491222-69491241 MS.gene54312:CDS 30.0%
!! CAGAAAGAATGATTTGTTGA+GGG - chr2.3:69492532-69492551 None:intergenic 30.0%
!! TCAGAAAGAATGATTTGTTG+AGG - chr2.3:69492533-69492552 None:intergenic 30.0%
!! TCTGATTTTAAAAAGGCTAG+CGG + chr2.3:69491742-69491761 MS.gene54312:intron 30.0%
!! TGACTTACGTTTTGAATGAA+AGG + chr2.3:69492749-69492768 MS.gene54312:intron 30.0%
!! TGTTTAACTTTTGTTGGTGA+TGG - chr2.3:69492245-69492264 None:intergenic 30.0%
!!! ATCAACAAACTCATTGGTTT+TGG + chr2.3:69491310-69491329 MS.gene54312:CDS 30.0%
AAGTCCCCTCCAATAATTAA+TGG + chr2.3:69491356-69491375 MS.gene54312:CDS 35.0%
AATGCAGATGAAGCAAGTAA+AGG + chr2.3:69493015-69493034 MS.gene54312:CDS 35.0%
ACAGAGACATTTGATATGCT+CGG - chr2.3:69491821-69491840 None:intergenic 35.0%
AGCACCAAATCTATATGAAG+TGG - chr2.3:69491261-69491280 None:intergenic 35.0%
AGCTGAAGTTTGAAGATTGA+TGG + chr2.3:69491973-69491992 MS.gene54312:intron 35.0%
CATATACATTTGACCAGTCA+AGG - chr2.3:69492287-69492306 None:intergenic 35.0%
CATTGAAATTCATGCAGCAT+TGG - chr2.3:69492044-69492063 None:intergenic 35.0%
CCGCTAAAGTCAACTATATA+AGG + chr2.3:69492311-69492330 MS.gene54312:intron 35.0%
CTCTCCATTAATTATTGGAG+GGG - chr2.3:69491363-69491382 None:intergenic 35.0%
CTGTTATTAATGGTAGCTGA+TGG + chr2.3:69492924-69492943 MS.gene54312:intron 35.0%
CTTAGATCAGTTAGAAGTTC+TGG - chr2.3:69493138-69493157 None:intergenic 35.0%
CTTTACTAGCTGAGGTTATT+AGG + chr2.3:69492971-69492990 MS.gene54312:CDS 35.0%
GCTGATGGTATTCTTATAGA+TGG + chr2.3:69492939-69492958 MS.gene54312:intron 35.0%
TAACTTTACCTGACCAATGA+AGG + chr2.3:69492661-69492680 MS.gene54312:intron 35.0%
TATTAATAGCTGTCTACAGC+TGG + chr2.3:69492561-69492580 MS.gene54312:intron 35.0%
TGGTTGCTTAATTCCTTCAT+TGG - chr2.3:69492677-69492696 None:intergenic 35.0%
TTAAATTAAGGGTCCTTGAC+TGG + chr2.3:69492271-69492290 MS.gene54312:intron 35.0%
TTCACTTCATCATCATCTGT+TGG - chr2.3:69493051-69493070 None:intergenic 35.0%
! AAATATCAACTTTGGGAGAC+AGG + chr2.3:69493199-69493218 MS.gene54312:CDS 35.0%
! AATATCAACTTTGGGAGACA+GGG + chr2.3:69493200-69493219 MS.gene54312:CDS 35.0%
! AATGATCCACCATTTTCACA+TGG - chr2.3:69493081-69493100 None:intergenic 35.0%
! CTTGACTGGTCAAATGTATA+TGG + chr2.3:69492285-69492304 MS.gene54312:intron 35.0%
! TATTATACCTTTGGAGAGGA+GGG - chr2.3:69491500-69491519 None:intergenic 35.0%
! TTATTATACCTTTGGAGAGG+AGG - chr2.3:69491501-69491520 None:intergenic 35.0%
!!! GTCATTTACGATGTCGATTT+TGG + chr2.3:69492868-69492887 MS.gene54312:intron 35.0%
!!! TTGGTTTTGGATAGTGAGAA+AGG + chr2.3:69491323-69491342 MS.gene54312:CDS 35.0%
AACTGAGTTCTGTACTGGAA+AGG + chr2.3:69493310-69493329 MS.gene54312:CDS 40.0%
ACATCACCAGCTGAATATTC+TGG + chr2.3:69492506-69492525 MS.gene54312:intron 40.0%
ACTGAGTTCTGTACTGGAAA+GGG + chr2.3:69493311-69493330 MS.gene54312:CDS 40.0%
CAAAGCAACTTTACTAGCTG+AGG + chr2.3:69492963-69492982 MS.gene54312:CDS 40.0%
CAAATGAGACTTCAAGAGGT+GGG + chr2.3:69491282-69491301 MS.gene54312:CDS 40.0%
CAGAGACATTTGATATGCTC+GGG - chr2.3:69491820-69491839 None:intergenic 40.0%
GCTTAATTCCTTCATTGGTC+AGG - chr2.3:69492672-69492691 None:intergenic 40.0%
GCTTCAAATGAGACTTCAAG+AGG + chr2.3:69491278-69491297 MS.gene54312:CDS 40.0%
GTTGAACCATGTGAAAATGG+TGG + chr2.3:69493072-69493091 MS.gene54312:CDS 40.0%
TCAAATGAGACTTCAAGAGG+TGG + chr2.3:69491281-69491300 MS.gene54312:CDS 40.0%
TCTTAACGAGTAATCCATCG+AGG - chr2.3:69493343-69493362 None:intergenic 40.0%
!! ACAATAAGTGCTGAGAAGCA+AGG + chr2.3:69491773-69491792 MS.gene54312:intron 40.0%
!! AGTGGCTCTGAAGAATCATA+GGG + chr2.3:69491412-69491431 MS.gene54312:CDS 40.0%
!! TAGTGGCTCTGAAGAATCAT+AGG + chr2.3:69491411-69491430 MS.gene54312:CDS 40.0%
AGAATCATAGGGAAGCAGAG+AGG + chr2.3:69491423-69491442 MS.gene54312:CDS 45.0%
TACAAGCTATGGGACAGAAC+TGG + chr2.3:69491999-69492018 MS.gene54312:intron 45.0%
TCTTCGATGGTCCCAAACTA+AGG + chr2.3:69492413-69492432 MS.gene54312:intron 45.0%
TGCATCTGTTTGCAAAGACG+TGG - chr2.3:69493369-69493388 None:intergenic 45.0%
!! AAAAAATAAATAAATAAGAT+AGG + chr2.3:69492714-69492733 MS.gene54312:intron 5.0%
!! AAACATTATAATTAAATTAA+GGG + chr2.3:69492260-69492279 MS.gene54312:intron 5.0%
!! TAAACATTATAATTAAATTA+AGG + chr2.3:69492259-69492278 MS.gene54312:intron 5.0%
!!! TCTTATTTATTTATTTTTTA+AGG - chr2.3:69492713-69492732 None:intergenic 5.0%
ACTTGTTCCCTCCTCTCCAA+AGG + chr2.3:69491490-69491509 MS.gene54312:CDS 50.0%
CCAGGAACTGAGTTCTGTAC+TGG + chr2.3:69493305-69493324 MS.gene54312:CDS 50.0%
CCAGTACAGAACTCAGTTCC+TGG - chr2.3:69493308-69493327 None:intergenic 50.0%
GGAAAGGGTTTCTACCTCGA+TGG + chr2.3:69493326-69493345 MS.gene54312:CDS 50.0%
TGAGGGCCAGAATATTCAGC+TGG - chr2.3:69492515-69492534 None:intergenic 50.0%
! CAGCTGGTGATGTGAAGTGT+GGG - chr2.3:69492499-69492518 None:intergenic 50.0%
! TCAGCTGGTGATGTGAAGTG+TGG - chr2.3:69492500-69492519 None:intergenic 50.0%
GAGAGGAGGGAACAAGTGCA+CGG - chr2.3:69491487-69491506 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 69491218 69493434 69491218 ID=MS.gene54312
chr2.3 mRNA 69491218 69493434 69491218 ID=MS.gene54312.t1;Parent=MS.gene54312
chr2.3 exon 69491218 69491511 69491218 ID=MS.gene54312.t1.exon1;Parent=MS.gene54312.t1
chr2.3 CDS 69491218 69491511 69491218 ID=cds.MS.gene54312.t1;Parent=MS.gene54312.t1
chr2.3 exon 69492958 69493434 69492958 ID=MS.gene54312.t1.exon2;Parent=MS.gene54312.t1
chr2.3 CDS 69492958 69493434 69492958 ID=cds.MS.gene54312.t1;Parent=MS.gene54312.t1
Gene Sequence

>MS.gene54312

ATGGATTATAGCTCTGATATTTCGAGGATACTATTCAATGCCACTTCATATAGATTTGGTGCTTCAAATGAGACTTCAAGAGGTGGGTTTGTATCAACAAACTCATTGGTTTTGGATAGTGAGAAAGGAGAACTAGTGAAGTCCCCTCCAATAATTAATGGAGAGAAAAAGATTTGTGATGCTAACAAAACTTTAGTGGCTCTGAAGAATCATAGGGAAGCAGAGAGGAAGAGAAGAAACAGAATTAATGGTCATCTTGCTAAACTCCGTGCACTTGTTCCCTCCTCTCCAAAGGTATAATAATCTTCCTTTCTTTTCGTATTTTTTATATGCATCCAATCATATTCGATCATTGTGTCACTTCGTTATATTTTTTTAAGGGAAAATGTTACTTCGTTATATTTGTTTTTAAAATATCTTTGTGGTGAAATGTGATTTTTTATAAACATGTTTTACACCGATGGTTTACCAATTGAACTTTTTTACAGTTTTATTTTGATTGTATAATTGTTTTAATATTGTTATCTGATTTTAAAAAGGCTAGCGGTGATACTTACAATAAGTGCTGAGAAGCAAGGAATTGCAGATACAAACTTGCACCCGAGCATATCAAATGTCTCTGTAACATTTTATCATCTAAGCTCTACTTACATGACATTGTTTTAGCATGTTTAAAATAATCAACATATATGATTCTGATTTAATTGCTCTGACTGAAAAAATACATTTACTTAGGTAACATTTAGAAATTTAGAAGCTGAAGTTTGAAGATTGATGGAAATACAAGCTATGGGACAGAACTGGTAGTACACGATATCATCACCCAATGCTGCATGAATTTCAATGTTCAATTGATTCATGTTTCTTTATTTACTCATGATTCATGCGTTCATTCATTTTGTACACCAATTAAGTTGCAATTAGGAATTTTGATTAAAATAAAACATGAGTAATCAATTGTACTACTACGTATACTACTGCTATATCATAACAACAATATCAAAATCATTTCAGCAAAAAATGACCATCACCAACAAAAGTTAAACATTATAATTAAATTAAGGGTCCTTGACTGGTCAAATGTATATGGGAACCGCTAAAGTCAACTATATAAGGTTGTGGAGTTGCATTTCAAAATTCTATAGTACTATAATGTAACATCATATTGGTTAGTTTATTCTTTTTTGCAGATTTATCTTCGATGGTCCCAAACTAAGGTTGTTTCATTTATTATTCAATATAATAATAAAGAAATAACATATGATTCTTAGTTGTGGGCCCACACTTCACATCACCAGCTGAATATTCTGGCCCTCAACAAATCATTCTTTCTGAATGTTTTTTATTAATAGCTGTCTACAGCTGGTTAAGCTATTCAAATAATTTATGCAATTTTACTACTAGTATTAATTTATTTGTTGTGGTGGGACTAAGTCTAGAAAATAACTTTACCTGACCAATGAAGGAATTAAGCAACCAACCATTTAATAATCCTTAAAAAATAAATAAATAAGATAGGACATGCTACCTATGACTTACGTTTTGAATGAAAGGCGTATCTGATTTCTAATATGTGTTAGGGTTTAATATCGGCACACATGATTACATTTACTCAATTTTAATTTATTATATTATTAATGCTGTCTATGTGTCATTTACGATGTCGATTTTGGTGTCAATGTTTCATAGTTTCACATATGTACATACTGTTATTAATGGTAGCTGATGGTATTCTTATAGATGGACAAAGCAACTTTACTAGCTGAGGTTATTAGGCAAGTGAAGCATTTGAAAAAAAATGCAGATGAAGCAAGTAAAGGTTATTCAATTCCAACAGATGATGATGAAGTGAAAGTTGAACCATGTGAAAATGGTGGATCATTTCTTTACAAAGCATCTATCAGCTGCGATTATCGACCAGAACTTCTAACTGATCTAAGACAAACTCTTGATAAACTTCAACTACAATTGGTAAGGGAAGAAATATCAACTTTGGGAGACAGGGTTAAGAATGAATTTGTCTACACATGTTGTAATGTAGACATTTATGATATTGAACAATGTCAACTTATTGGAAATAATGTTCACCAGGAACTGAGTTCTGTACTGGAAAGGGTTTCTACCTCGATGGATTACTCGTTAAGAGCACCACGTCTTTGCAAACAGATGCAGCAGACATCTACTTTGTCATGCAACCATGAGTTTTGCTCTTGTTGA
Protein sequence

>MS.gene54312.t1

MDYSSDISRILFNATSYRFGASNETSRGGFVSTNSLVLDSEKGELVKSPPIINGEKKICDANKTLVALKNHREAERKRRNRINGHLAKLRALVPSSPKMDKATLLAEVIRQVKHLKKNADEASKGYSIPTDDDEVKVEPCENGGSFLYKASISCDYRPELLTDLRQTLDKLQLQLVREEISTLGDRVKNEFVYTCCNVDIYDIEQCQLIGNNVHQELSSVLERVSTSMDYSLRAPRLCKQMQQTSTLSCNHEFCSC