AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54314.t1 MTR_2g015850 92.388 289 21 1 40 328 77 364 0.0 535
MS.gene54314.t1 MTR_4g107930 53.474 331 148 4 1 327 16 344 5.05e-122 354
MS.gene54314.t1 MTR_5g022560 54.268 328 142 5 4 327 20 343 7.98e-122 354
MS.gene54314.t1 MTR_4g079770 54.769 325 139 6 8 327 23 344 2.41e-121 352
MS.gene54314.t1 MTR_4g079470 54.769 325 139 6 8 327 23 344 5.82e-121 352
MS.gene54314.t1 MTR_4g079800 53.846 325 142 6 8 327 23 344 1.17e-120 351
MS.gene54314.t1 MTR_4g047610 53.086 324 145 5 8 327 22 342 8.64e-116 338
MS.gene54314.t1 MTR_2g075830 50.150 333 153 4 1 327 15 340 5.96e-109 321
MS.gene54314.t1 MTR_4g080360 47.416 329 168 5 1 326 14 340 2.23e-108 320
MS.gene54314.t1 MTR_4g080730 49.844 321 151 6 11 327 25 339 1.96e-104 309
MS.gene54314.t1 MTR_4g080700 48.910 321 154 6 11 327 25 339 2.37e-103 306
MS.gene54314.t1 MTR_1g107550 48.734 316 153 6 17 326 42 354 9.22e-97 294
MS.gene54314.t1 MTR_3g102220 46.584 322 162 7 13 326 41 360 1.85e-95 291
MS.gene54314.t1 MTR_4g079440 46.562 320 153 7 18 328 35 345 3.07e-95 286
MS.gene54314.t1 MTR_7g078600 46.474 312 154 4 20 328 37 338 2.28e-94 284
MS.gene54314.t1 MTR_2g083930 44.479 317 168 4 19 328 29 344 5.19e-94 283
MS.gene54314.t1 MTR_2g090890 43.902 328 173 5 2 326 16 335 1.58e-93 281
MS.gene54314.t1 MTR_3g116080 45.860 314 162 6 17 327 39 347 3.93e-93 281
MS.gene54314.t1 MTR_1g018840 46.154 312 160 6 21 326 51 360 8.76e-93 283
MS.gene54314.t1 MTR_2g015840 84.000 150 24 0 1 150 9 158 1.49e-92 276
MS.gene54314.t1 MTR_2g015840 54.839 155 65 1 179 328 118 272 4.08e-53 176
MS.gene54314.t1 MTR_1g018840 46.154 312 160 6 21 326 51 360 1.86e-92 283
MS.gene54314.t1 MTR_4g094918 44.795 317 167 6 17 327 27 341 4.37e-92 278
MS.gene54314.t1 MTR_3g075390 45.541 314 165 5 17 326 32 343 1.07e-91 278
MS.gene54314.t1 MTR_3g112430 46.405 306 159 4 26 327 37 341 1.29e-91 280
MS.gene54314.t1 MTR_2g067900 47.706 327 154 8 12 325 1 323 4.94e-91 275
MS.gene54314.t1 MTR_4g104370 46.349 315 160 7 17 326 32 342 1.90e-90 275
MS.gene54314.t1 MTR_7g079230 43.114 334 173 7 1 328 14 336 8.28e-89 270
MS.gene54314.t1 MTR_2g067560 49.240 329 140 11 12 320 1 322 8.50e-89 285
MS.gene54314.t1 MTR_2g066980 44.092 347 139 10 1 328 9 319 3.25e-88 267
MS.gene54314.t1 MTR_2g064475 44.290 359 149 13 12 327 1 351 3.46e-87 266
MS.gene54314.t1 MTR_2g016180 41.231 325 175 7 12 327 38 355 2.18e-74 237
MS.gene54314.t1 MTR_2g016270 41.304 322 164 7 12 327 38 340 1.25e-72 232
MS.gene54314.t1 MTR_1g023810 48.954 239 115 6 91 325 26 261 6.04e-72 224
MS.gene54314.t1 MTR_2g015920 37.423 326 191 7 10 327 38 358 1.48e-68 219
MS.gene54314.t1 MTR_4g125300 40.673 327 169 12 12 328 38 349 1.01e-66 213
MS.gene54314.t1 MTR_4g065077 40.798 326 176 10 12 327 38 356 3.18e-66 213
MS.gene54314.t1 MTR_4g071060 38.438 320 169 9 10 308 29 341 5.08e-63 204
MS.gene54314.t1 MTR_1g023210 39.322 295 156 8 31 308 57 345 3.39e-62 202
MS.gene54314.t1 MTR_1g094135 39.297 313 164 11 28 321 59 364 4.47e-61 199
MS.gene54314.t1 MTR_8g086470 38.769 325 168 10 12 327 54 356 2.80e-60 197
MS.gene54314.t1 MTR_2g015930 35.562 329 194 10 10 327 37 358 1.07e-57 190
MS.gene54314.t1 MTR_7g078630 39.373 287 130 6 14 287 695 950 1.74e-53 188
MS.gene54314.t1 MTR_4g072790 34.323 303 165 9 10 308 33 305 4.29e-46 159
MS.gene54314.t1 MTR_4g073660 32.573 307 169 9 10 308 32 308 1.36e-43 152
MS.gene54314.t1 MTR_4g072340 32.573 307 169 9 10 308 33 309 1.39e-43 152
MS.gene54314.t1 MTR_1g025450 41.538 195 91 7 91 280 32 208 3.38e-38 142
MS.gene54314.t1 MTR_7g078580 53.608 97 45 0 230 326 4 100 3.32e-31 114
MS.gene54314.t1 MTR_7g111060 29.180 305 179 13 42 319 36 330 4.67e-27 109
MS.gene54314.t1 MTR_7g079130 53.846 91 41 1 237 326 6 96 4.77e-27 102
MS.gene54314.t1 MTR_7g111050 27.844 334 189 14 30 327 20 337 2.34e-26 107
MS.gene54314.t1 MTR_1g042480 55.556 90 36 2 1 86 9 98 1.50e-25 99.8
MS.gene54314.t1 MTR_1g042360 55.556 90 36 2 1 86 9 98 1.50e-25 99.8
MS.gene54314.t1 MTR_3g029560 31.579 209 120 3 124 328 49 238 2.01e-25 102
MS.gene54314.t1 MTR_1g054590 54.762 84 33 3 1 80 8 90 7.97e-22 89.0
MS.gene54314.t1 MTR_7g078310 38.732 142 74 5 65 202 113 245 4.42e-21 91.3
MS.gene54314.t1 MTR_1g009480 52.326 86 36 3 1 82 10 94 1.60e-19 84.0
MS.gene54314.t1 MTR_4g023770 52.381 84 31 3 1 80 8 86 1.71e-19 82.4
MS.gene54314.t1 MTR_2g016380 39.024 123 68 5 209 327 16 135 2.97e-18 83.2
MS.gene54314.t1 MTR_8g087510 35.433 127 80 2 154 279 23 148 4.34e-17 78.2
MS.gene54314.t1 MTR_7g111050 26.502 283 163 11 40 289 26 296 5.79e-17 80.5
MS.gene54314.t1 MTR_2g047960 45.455 88 47 1 117 203 2 89 1.06e-16 74.7
MS.gene54314.t1 MTR_1g025370 38.202 89 41 2 1 76 10 97 2.14e-15 71.2
MS.gene54314.t1 MTR_7g079260 48.611 72 21 2 253 324 7 62 5.78e-14 67.8
MS.gene54314.t1 MTR_3g082370 27.143 210 126 9 117 306 4 206 5.07e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54314.t1 AT2G34080 54.037 322 139 5 13 327 25 344 9.33e-121 351
MS.gene54314.t1 AT2G27420 52.795 322 142 5 15 326 25 346 7.69e-115 336
MS.gene54314.t1 AT3G49340 50.296 338 153 5 2 326 4 339 5.51e-114 334
MS.gene54314.t1 AT1G29090 52.188 320 144 6 15 327 37 354 1.80e-110 325
MS.gene54314.t1 AT1G29080 52.063 315 143 5 19 327 33 345 2.49e-110 325
MS.gene54314.t1 AT5G45890 48.024 329 163 5 7 328 19 346 1.48e-108 320
MS.gene54314.t1 AT1G29110 48.137 322 144 9 15 328 28 334 9.80e-97 290
MS.gene54314.t1 AT3G19390 46.440 323 153 6 15 327 33 345 1.92e-96 293
MS.gene54314.t1 AT1G20850 47.452 314 159 6 17 327 43 353 4.18e-96 289
MS.gene54314.t1 AT5G43060 47.468 316 155 6 17 326 42 352 7.48e-96 292
MS.gene54314.t1 AT4G35350 47.588 311 154 6 21 327 47 352 1.49e-94 285
MS.gene54314.t1 AT3G48340 45.646 333 171 8 1 326 13 342 5.69e-94 283
MS.gene54314.t1 AT5G50260 43.824 340 175 6 1 326 4 341 2.63e-93 282
MS.gene54314.t1 AT4G23520 49.346 306 145 8 26 326 48 348 1.36e-92 280
MS.gene54314.t1 AT1G09850 46.429 308 160 4 21 326 28 332 4.67e-92 281
MS.gene54314.t1 AT1G47128 44.164 317 163 8 17 326 42 351 1.15e-90 278
MS.gene54314.t1 AT4G36880 43.598 328 173 7 10 326 34 360 3.52e-90 274
MS.gene54314.t1 AT3G19400 45.426 317 163 6 17 327 36 348 6.01e-90 273
MS.gene54314.t1 AT4G11310 43.519 324 154 8 17 326 43 351 7.60e-90 273
MS.gene54314.t1 AT4G11320 43.810 315 150 7 26 326 57 358 1.04e-88 270
MS.gene54314.t1 AT4G11320 43.810 315 150 7 26 326 57 358 1.04e-88 270
MS.gene54314.t1 AT3G48350 44.620 316 168 6 17 327 30 343 2.58e-86 264
MS.gene54314.t1 AT1G06260 44.109 331 168 8 7 326 17 341 3.56e-82 253
MS.gene54314.t1 AT3G43960 43.849 317 167 8 17 326 33 345 5.69e-80 248
MS.gene54314.t1 AT5G60360 42.593 324 167 11 12 328 46 357 1.98e-70 223
MS.gene54314.t1 AT5G60360 41.975 324 168 10 12 328 46 356 8.24e-69 219
MS.gene54314.t1 AT5G60360 42.384 302 159 9 12 306 46 339 5.25e-66 212
MS.gene54314.t1 AT4G35350 46.694 242 121 5 21 258 47 284 4.51e-65 207
MS.gene54314.t1 AT4G39090 38.644 295 158 8 31 308 57 345 1.33e-64 208
MS.gene54314.t1 AT3G45310 40.432 324 170 11 14 328 48 357 3.65e-63 204
MS.gene54314.t1 AT3G45310 39.815 324 171 10 14 328 48 356 1.88e-61 200
MS.gene54314.t1 AT2G21430 37.500 312 169 10 17 308 37 342 4.53e-61 199
MS.gene54314.t1 AT3G19400 43.089 246 131 5 17 256 36 278 1.75e-59 193
MS.gene54314.t1 AT3G54940 40.066 302 152 12 28 308 54 347 1.77e-58 192
MS.gene54314.t1 AT4G16190 38.889 306 162 8 31 319 61 358 2.35e-58 192
MS.gene54314.t1 AT3G48350 42.449 245 134 6 17 256 30 272 2.37e-54 180
MS.gene54314.t1 AT3G54940 38.428 229 123 7 28 244 54 276 1.72e-42 148
MS.gene54314.t1 AT4G01610 28.378 296 171 11 54 319 46 330 2.71e-28 112
MS.gene54314.t1 AT1G02305 27.609 297 180 9 54 321 49 339 5.21e-28 112
MS.gene54314.t1 AT4G01610 27.703 296 173 11 54 319 46 330 2.38e-26 107
MS.gene54314.t1 AT1G02300 30.476 210 119 7 132 319 146 350 3.00e-20 90.9
MS.gene54314.t1 AT2G22160 40.625 96 46 4 41 132 10 98 5.30e-13 65.1

Find 66 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCATTTGATCATTTAAT+TGG 0.195265 2.3:+69519188 None:intergenic
TTGTTGGAATATCATCATTC+AGG 0.284103 2.3:+69520240 None:intergenic
TACATGGGAGATGTTTATTC+AGG 0.305565 2.3:-69519046 MS.gene54314:CDS
ATTGGTTGATTAAGAATTCT+TGG 0.312011 2.3:-69518952 MS.gene54314:CDS
TTAAATCAGAGTATGGATTT+AGG 0.312768 2.3:+69520354 None:intergenic
CAGCAATGGATGATAAAATA+TGG 0.313356 2.3:-69520488 MS.gene54314:CDS
TCACTAGTTAAATCAGAGTA+TGG 0.325666 2.3:+69520347 None:intergenic
GAGTTTATTGCTTCACATAC+TGG 0.326687 2.3:-69520323 MS.gene54314:CDS
AGAATTCTTGGGGTAAAGAT+TGG 0.334902 2.3:-69518940 MS.gene54314:CDS
GTCTGACTACCTTCTTGATA+TGG 0.336745 2.3:+69519214 None:intergenic
GACAGTGTTCTTGACATGAT+TGG 0.347406 2.3:+69520536 None:intergenic
TTGGTTGATTAAGAATTCTT+GGG 0.374775 2.3:-69518951 MS.gene54314:CDS
GAATTCTTGGGGTAAAGATT+GGG 0.376941 2.3:-69518939 MS.gene54314:CDS
CTCATTATCTGAGCAACAAT+TGG 0.401955 2.3:-69519338 MS.gene54314:CDS
ACTGCAATGCCAACTGATAC+TGG 0.406208 2.3:+69519088 None:intergenic
TCAGTCGTGGCTGCTGTAGA+AGG 0.411115 2.3:-69519391 MS.gene54314:CDS
TAGCAGTTGTTGTTCATCAT+TGG 0.413217 2.3:+69519129 None:intergenic
TGTTTGCATGATTATCTTGT+GGG 0.419752 2.3:-69520570 None:intergenic
TGCCTCCAATCAAAATTTGT+TGG 0.420797 2.3:+69520224 None:intergenic
TGGGTGTGATGGAGGTAATA+TGG 0.436333 2.3:-69519293 MS.gene54314:CDS
GGTTTGTATTATATATTTGA+AGG 0.448659 2.3:+69519264 None:intergenic
ACAGTGTTCTTGACATGATT+GGG 0.450300 2.3:+69520537 None:intergenic
GGTAAAGATTGGGGTGAAGA+AGG 0.460104 2.3:-69518929 MS.gene54314:CDS
TGATGAATTTCAACATTACA+TGG 0.464191 2.3:-69519062 MS.gene54314:CDS
TGTCTCACTAACAATACCTT+GGG 0.465163 2.3:+69519243 None:intergenic
AATATCATCATTCAGGTTGA+AGG 0.470988 2.3:+69520247 None:intergenic
GCAGTTACTGCAGTTGGTTA+CGG 0.478465 2.3:-69518998 MS.gene54314:CDS
TACTGCAACTGACCTCATCT+TGG 0.480728 2.3:+69520271 None:intergenic
CTGTCTCACTAACAATACCT+TGG 0.493668 2.3:+69519242 None:intergenic
AGTTGTTGTTCATCATTGGC+AGG 0.496843 2.3:+69519133 None:intergenic
AACCATGCAGTTACTGCAGT+TGG 0.506721 2.3:-69519004 MS.gene54314:CDS
AGGTTACATGAAGTTGTTGA+GGG 0.508816 2.3:-69518909 MS.gene54314:CDS
GCAATGCCACATTGACCTCC+TGG 0.513337 2.3:+69518875 None:intergenic
CTGTTTGCATGATTATCTTG+TGG 0.513337 2.3:-69520571 None:intergenic
ATAGAGAATTTCAACAATGC+TGG 0.520071 2.3:-69520398 MS.gene54314:CDS
TTGAGGGAAAGTGGTGAACC+AGG 0.520538 2.3:-69518893 MS.gene54314:CDS
AATTCTTGGGGTAAAGATTG+GGG 0.530447 2.3:-69518938 MS.gene54314:CDS
GGTTACGGTGTAAGTGAAGA+TGG 0.532156 2.3:-69518983 MS.gene54314:CDS
GTGAAGATGGAACTAAGTAT+TGG 0.533019 2.3:-69518970 MS.gene54314:CDS
AAGACATACCACAACTGCCT+TGG 0.539526 2.3:+69520174 None:intergenic
ATGAAGTTGTTGAGGGAAAG+TGG 0.542145 2.3:-69518902 MS.gene54314:CDS
TGCAACTGACCTCATCTTGG+AGG 0.547643 2.3:+69520274 None:intergenic
GTTAAAGACCAAGGCAGTTG+TGG 0.558254 2.3:-69520182 MS.gene54314:intron
ACTGCCTTGGTCTTTAACAT+CGG 0.570004 2.3:+69520187 None:intergenic
GAACAGAACAATGGGTGTGA+TGG 0.572310 2.3:-69519304 MS.gene54314:CDS
AAGGTTACATGAAGTTGTTG+AGG 0.582698 2.3:-69518910 MS.gene54314:CDS
ACTGCGATGAACAGAACAAT+GGG 0.582764 2.3:-69519312 MS.gene54314:CDS
ATATCATCATTCAGGTTGAA+GGG 0.586071 2.3:+69520248 None:intergenic
TGGTTGATTAAGAATTCTTG+GGG 0.586376 2.3:-69518950 MS.gene54314:CDS
ACAGATTATCCATATCAAGA+AGG 0.601748 2.3:-69519223 MS.gene54314:CDS
GATGTTTATTCAGGAACATG+TGG 0.602261 2.3:-69519037 MS.gene54314:CDS
AACCAACTGCAGTAACTGCA+TGG 0.607601 2.3:+69519002 None:intergenic
GGTAACAAGAGTTACAAGCT+TGG 0.620609 2.3:-69520377 MS.gene54314:CDS
GTATCAGTTGGCATTGCAGT+CGG 0.623362 2.3:-69519085 MS.gene54314:CDS
GATGAATTTCAACATTACAT+GGG 0.623570 2.3:-69519061 MS.gene54314:CDS
TTGTTGAAGCACATCAGCAA+TGG 0.625595 2.3:-69520502 MS.gene54314:CDS
GACTTTCATCCTCCAAGATG+AGG 0.628604 2.3:-69520283 MS.gene54314:CDS
GCACAACAACCAGTATCAGT+TGG 0.635741 2.3:-69519097 MS.gene54314:CDS
GGTGCTGTGAAAATCAAGAC+TGG 0.639937 2.3:-69519370 MS.gene54314:CDS
CAGAACAATGGGTGTGATGG+AGG 0.644692 2.3:-69519301 MS.gene54314:CDS
AAATATATAATACAAACCCA+AGG 0.655643 2.3:-69519259 MS.gene54314:CDS
TCATCTTGGAGGATGAAAGT+CGG 0.661178 2.3:+69520285 None:intergenic
GGTGAACCAGGAGGTCAATG+TGG 0.664219 2.3:-69518881 MS.gene54314:CDS
GACTGCGATGAACAGAACAA+TGG 0.669619 2.3:-69519313 MS.gene54314:CDS
GTTACCGATGTTAAAGACCA+AGG 0.675798 2.3:-69520191 MS.gene54314:CDS
AGGGAAAGTGGTGAACCAGG+AGG 0.728920 2.3:-69518890 MS.gene54314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTTACATTTTAATATTA+AGG - chr2.3:69519833-69519852 MS.gene54314:intron 10.0%
!!! TTTTGTGATATAAAATAAAA+TGG + chr2.3:69519443-69519462 None:intergenic 10.0%
!! AAATATCGAAAAGTTATTTA+AGG - chr2.3:69519722-69519741 MS.gene54314:intron 15.0%
!! ATTTCATTTGATCATTTAAT+TGG + chr2.3:69520225-69520244 None:intergenic 15.0%
!! TGATATAAAATAAAATGGAA+TGG + chr2.3:69519438-69519457 None:intergenic 15.0%
!!! TATTTTTTAATTAGTCATCT+TGG - chr2.3:69519276-69519295 MS.gene54314:CDS 15.0%
!!! TTTAGATTTTTCTTTTTTGA+TGG + chr2.3:69519666-69519685 None:intergenic 15.0%
!! AAATATATAATACAAACCCA+AGG - chr2.3:69520151-69520170 MS.gene54314:intron 20.0%
!! GGTTTGTATTATATATTTGA+AGG + chr2.3:69520149-69520168 None:intergenic 20.0%
!!! AATAACTTTTCGATATTTGA+TGG + chr2.3:69519720-69519739 None:intergenic 20.0%
!!! ATATTCCAACAAATTTTGAT+TGG - chr2.3:69519181-69519200 MS.gene54314:CDS 20.0%
!!! TATCATTCAATATGTTTTGT+AGG - chr2.3:69519980-69519999 MS.gene54314:intron 20.0%
! ACAATGCATAAAAATATGCA+AGG - chr2.3:69519618-69519637 MS.gene54314:intron 25.0%
! ATTGGTTGATTAAGAATTCT+TGG - chr2.3:69520458-69520477 MS.gene54314:CDS 25.0%
! GATGAATTTCAACATTACAT+GGG - chr2.3:69520349-69520368 MS.gene54314:CDS 25.0%
! GCATTATTGATGTGTTTATA+TGG + chr2.3:69519517-69519536 None:intergenic 25.0%
! TATGCTTCATTAAAAGTTCT+CGG + chr2.3:69519478-69519497 None:intergenic 25.0%
! TGATGAATTTCAACATTACA+TGG - chr2.3:69520348-69520367 MS.gene54314:CDS 25.0%
! TGATGTGTTTATATGGTATT+TGG + chr2.3:69519510-69519529 None:intergenic 25.0%
! TTAAATCAGAGTATGGATTT+AGG + chr2.3:69519059-69519078 None:intergenic 25.0%
! TTGGTTGATTAAGAATTCTT+GGG - chr2.3:69520459-69520478 MS.gene54314:CDS 25.0%
!!! AGATTTTTCTTTTTTGATGG+TGG + chr2.3:69519663-69519682 None:intergenic 25.0%
AACAAAGTCTCCAATATGTT+TGG - chr2.3:69519339-69519358 MS.gene54314:CDS 30.0%
ACAGATTATCCATATCAAGA+AGG - chr2.3:69520187-69520206 MS.gene54314:CDS 30.0%
ATAAAAATATGCAAGGTCTG+GGG - chr2.3:69519625-69519644 MS.gene54314:intron 30.0%
ATAGAGAATTTCAACAATGC+TGG - chr2.3:69519012-69519031 MS.gene54314:CDS 30.0%
CAGCAATGGATGATAAAATA+TGG - chr2.3:69518922-69518941 MS.gene54314:CDS 30.0%
CATAAAAATATGCAAGGTCT+GGG - chr2.3:69519624-69519643 MS.gene54314:intron 30.0%
TACCGAAAATCCAAACATAT+TGG + chr2.3:69519352-69519371 None:intergenic 30.0%
TATTGGAGACTTTGTTAGTT+TGG + chr2.3:69519335-69519354 None:intergenic 30.0%
TCACTAGTTAAATCAGAGTA+TGG + chr2.3:69519066-69519085 None:intergenic 30.0%
TGGTTGATTAAGAATTCTTG+GGG - chr2.3:69520460-69520479 MS.gene54314:CDS 30.0%
TTGTTGGAATATCATCATTC+AGG + chr2.3:69519173-69519192 None:intergenic 30.0%
! ATGTTTTGTAGGATGTTGTT+GGG - chr2.3:69519991-69520010 MS.gene54314:intron 30.0%
! CTCCAATATGTTTGGATTTT+CGG - chr2.3:69519347-69519366 MS.gene54314:CDS 30.0%
! TATGTTTTGTAGGATGTTGT+TGG - chr2.3:69519990-69520009 MS.gene54314:intron 30.0%
! TGTTTGGATTTTCGGTATAA+AGG - chr2.3:69519355-69519374 MS.gene54314:CDS 30.0%
!! AATATCATCATTCAGGTTGA+AGG + chr2.3:69519166-69519185 None:intergenic 30.0%
!! ATATCATCATTCAGGTTGAA+GGG + chr2.3:69519165-69519184 None:intergenic 30.0%
!! TGTAATTTCTAGCCTTAGAT+AGG + chr2.3:69519864-69519883 None:intergenic 30.0%
!!! TTCCAACAAATTTTGATTGG+AGG - chr2.3:69519184-69519203 MS.gene54314:CDS 30.0%
AAAAGTTCTCGGAGGATATT+TGG + chr2.3:69519467-69519486 None:intergenic 35.0%
AAGGTTACATGAAGTTGTTG+AGG - chr2.3:69520500-69520519 MS.gene54314:CDS 35.0%
AATTCTTGGGGTAAAGATTG+GGG - chr2.3:69520472-69520491 MS.gene54314:CDS 35.0%
ACAGTGTTCTTGACATGATT+GGG + chr2.3:69518876-69518895 None:intergenic 35.0%
AGAATTCTTGGGGTAAAGAT+TGG - chr2.3:69520470-69520489 MS.gene54314:CDS 35.0%
AGGTTACATGAAGTTGTTGA+GGG - chr2.3:69520501-69520520 MS.gene54314:CDS 35.0%
ATTAAGGAACCACCTATCTA+AGG - chr2.3:69519849-69519868 MS.gene54314:intron 35.0%
CTCATTATCTGAGCAACAAT+TGG - chr2.3:69520072-69520091 MS.gene54314:intron 35.0%
GAATTCTTGGGGTAAAGATT+GGG - chr2.3:69520471-69520490 MS.gene54314:CDS 35.0%
GAGTTTATTGCTTCACATAC+TGG - chr2.3:69519087-69519106 MS.gene54314:CDS 35.0%
GATGTTTATTCAGGAACATG+TGG - chr2.3:69520373-69520392 MS.gene54314:CDS 35.0%
GCATAAAAATATGCAAGGTC+TGG - chr2.3:69519623-69519642 MS.gene54314:intron 35.0%
GGTATAAAGGTTGAATGACA+TGG - chr2.3:69519368-69519387 MS.gene54314:CDS 35.0%
GTGAAGATGGAACTAAGTAT+TGG - chr2.3:69520440-69520459 MS.gene54314:CDS 35.0%
TAATTAGTCATCTTGGAGCA+TGG - chr2.3:69519283-69519302 MS.gene54314:CDS 35.0%
TACATGGGAGATGTTTATTC+AGG - chr2.3:69520364-69520383 MS.gene54314:CDS 35.0%
TGCCTCCAATCAAAATTTGT+TGG + chr2.3:69519189-69519208 None:intergenic 35.0%
TGTCTCACTAACAATACCTT+GGG + chr2.3:69520170-69520189 None:intergenic 35.0%
! CTTTTTGCGTTTCTCTAACT+CGG + chr2.3:69518968-69518987 None:intergenic 35.0%
! TAGCAGTTGTTGTTCATCAT+TGG + chr2.3:69520284-69520303 None:intergenic 35.0%
!! AATTTCTAGCCTTAGATAGG+TGG + chr2.3:69519861-69519880 None:intergenic 35.0%
!!! AATTTTGATTGGAGGCAACA+AGG - chr2.3:69519192-69519211 MS.gene54314:CDS 35.0%
!!! TTTTCTTTTTTGATGGTGGC+CGG + chr2.3:69519659-69519678 None:intergenic 35.0%
ACTGCGATGAACAGAACAAT+GGG - chr2.3:69520098-69520117 MS.gene54314:intron 40.0%
ATGAAGTTGTTGAGGGAAAG+TGG - chr2.3:69520508-69520527 MS.gene54314:CDS 40.0%
CTGTCTCACTAACAATACCT+TGG + chr2.3:69520171-69520190 None:intergenic 40.0%
GACAGTGTTCTTGACATGAT+TGG + chr2.3:69518877-69518896 None:intergenic 40.0%
GCACAAACACTGATCGAATT+AGG + chr2.3:69519776-69519795 None:intergenic 40.0%
GCTTCATTAAAAGTTCTCGG+AGG + chr2.3:69519475-69519494 None:intergenic 40.0%
GGTAACAAGAGTTACAAGCT+TGG - chr2.3:69519033-69519052 MS.gene54314:CDS 40.0%
GTCTGACTACCTTCTTGATA+TGG + chr2.3:69520199-69520218 None:intergenic 40.0%
GTTACCGATGTTAAAGACCA+AGG - chr2.3:69519219-69519238 MS.gene54314:CDS 40.0%
TCATCTTGGAGGATGAAAGT+CGG + chr2.3:69519128-69519147 None:intergenic 40.0%
TTGTTGAAGCACATCAGCAA+TGG - chr2.3:69518908-69518927 MS.gene54314:CDS 40.0%
! ACTGCCTTGGTCTTTAACAT+CGG + chr2.3:69519226-69519245 None:intergenic 40.0%
! AGTTGTTGTTCATCATTGGC+AGG + chr2.3:69520280-69520299 None:intergenic 40.0%
!!! TTTCTTTTTTGATGGTGGCC+GGG + chr2.3:69519658-69519677 None:intergenic 40.0%
AACCAACTGCAGTAACTGCA+TGG + chr2.3:69520411-69520430 None:intergenic 45.0%
AACCATGCAGTTACTGCAGT+TGG - chr2.3:69520406-69520425 MS.gene54314:CDS 45.0%
AAGACATACCACAACTGCCT+TGG + chr2.3:69519239-69519258 None:intergenic 45.0%
ACTGCAATGCCAACTGATAC+TGG + chr2.3:69520325-69520344 None:intergenic 45.0%
GAACAGAACAATGGGTGTGA+TGG - chr2.3:69520106-69520125 MS.gene54314:intron 45.0%
GACTGCGATGAACAGAACAA+TGG - chr2.3:69520097-69520116 MS.gene54314:intron 45.0%
GACTTTCATCCTCCAAGATG+AGG - chr2.3:69519127-69519146 MS.gene54314:CDS 45.0%
GCACAACAACCAGTATCAGT+TGG - chr2.3:69520313-69520332 MS.gene54314:CDS 45.0%
GCAGTTACTGCAGTTGGTTA+CGG - chr2.3:69520412-69520431 MS.gene54314:CDS 45.0%
GGTAAAGATTGGGGTGAAGA+AGG - chr2.3:69520481-69520500 MS.gene54314:CDS 45.0%
GGTTACGGTGTAAGTGAAGA+TGG - chr2.3:69520427-69520446 MS.gene54314:CDS 45.0%
GTATCAGTTGGCATTGCAGT+CGG - chr2.3:69520325-69520344 MS.gene54314:CDS 45.0%
GTTAAAGACCAAGGCAGTTG+TGG - chr2.3:69519228-69519247 MS.gene54314:CDS 45.0%
TACTGCAACTGACCTCATCT+TGG + chr2.3:69519142-69519161 None:intergenic 45.0%
! GGTGCTGTGAAAATCAAGAC+TGG - chr2.3:69520040-69520059 MS.gene54314:intron 45.0%
! TGGGTGTGATGGAGGTAATA+TGG - chr2.3:69520117-69520136 MS.gene54314:intron 45.0%
! TTGTTGGGCATTTTCAGTCG+TGG - chr2.3:69520006-69520025 MS.gene54314:intron 45.0%
!!! TTCTTTTTTGATGGTGGCCG+GGG + chr2.3:69519657-69519676 None:intergenic 45.0%
!! AATAATAAATATAAACATAA+TGG - chr2.3:69519595-69519614 MS.gene54314:intron 5.0%
CAGAACAATGGGTGTGATGG+AGG - chr2.3:69520109-69520128 MS.gene54314:intron 50.0%
TGCAACTGACCTCATCTTGG+AGG + chr2.3:69519139-69519158 None:intergenic 50.0%
TTGAGGGAAAGTGGTGAACC+AGG - chr2.3:69520517-69520536 MS.gene54314:CDS 50.0%
AGGGAAAGTGGTGAACCAGG+AGG - chr2.3:69520520-69520539 MS.gene54314:CDS 55.0%
GCAATGCCACATTGACCTCC+TGG + chr2.3:69520538-69520557 None:intergenic 55.0%
GGTGAACCAGGAGGTCAATG+TGG - chr2.3:69520529-69520548 MS.gene54314:CDS 55.0%
TCAGTCGTGGCTGCTGTAGA+AGG - chr2.3:69520019-69520038 MS.gene54314:intron 55.0%
! AAGGTCTGGGGTTCGAACCC+CGG - chr2.3:69519637-69519656 MS.gene54314:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 69518847 69520585 69518847 ID=MS.gene54314
chr2.3 mRNA 69518847 69520585 69518847 ID=MS.gene54314.t1;Parent=MS.gene54314
chr2.3 exon 69520183 69520585 69520183 ID=MS.gene54314.t1.exon1;Parent=MS.gene54314.t1
chr2.3 CDS 69520183 69520585 69520183 ID=cds.MS.gene54314.t1;Parent=MS.gene54314.t1
chr2.3 exon 69518847 69519430 69518847 ID=MS.gene54314.t1.exon2;Parent=MS.gene54314.t1
chr2.3 CDS 69518847 69519430 69518847 ID=cds.MS.gene54314.t1;Parent=MS.gene54314.t1
Gene Sequence

>MS.gene54314

ATGATTATCTTGTGGGCATGTGCATACCCAATCATGTCAAGAACACTGTCTGAATCATCTGTTGTTGAAGCACATCAGCAATGGATGATAAAATATGGACGCACATACACAAATAGTTCCGAGTTAGAGAAACGCAAAAAGATATTTAAAGAGAATTTAGAATACATAGAGAATTTCAACAATGCTGGTAACAAGAGTTACAAGCTTGGCCTAAATCCATACTCTGATTTAACTAGTGAAGAGTTTATTGCTTCACATACTGGACTCAAAGTTTCTGACCGACTTTCATCCTCCAAGATGAGGTCAGTTGCAGTACCCTTCAACCTGAATGATGATATTCCAACAAATTTTGATTGGAGGCAACAAGGAGCTGTTACCGATGTTAAAGACCAAGGCAGTTGTGGTATGTCTTAATTGTTTGATTTTATTTATTTTTTAATTAGTCATCTTGGAGCATGGAATTTTGAAACATAAAATACATGCATCCAAACTAACAAAGTCTCCAATATGTTTGGATTTTCGGTATAAAGGTTGAATGACATGGAATGAGTTCGTCAGATTTTATTACAGTATGTTACTCATTCTATTCCATTCCATTTTATTTTATATCACAAAATCCAAATATCCTCCGAGAACTTTTAATGAAGCATAACATAGATACCAAATACCATATAAACACATCAATAATGCTAATGTAACAAATATAGAGCACAAAAACTAATACATATTTGTTTATTTATTATTATTAAATAATAAATATAAACATAATGGACAATGCATAAAAATATGCAAGGTCTGGGGTTCGAACCCCGGCCACCATCAAAAAAGAAAAATCTAAATAAAAAATGCTAGTCTTCTATCTTTATTCATCCATCAAATATCGAAAAGTTATTTAAGGAACATCGATGTATGTCAGATCAAAGACACCTAATTCGATCAGTGTTTGTGCTAGATAGACTTTAATATTTGTTCTTTTGTTGAGATAAAAAGTTACATTTTAATATTAAGGAACCACCTATCTAAGGCTAGAAATTACATGTGACTTTATGAAAAGTCAACTTGATAAAAACTTTATGAAAAGTGACTTGTCTTATAAAGAAAAATTTAATTTAAGAATAAGCATATCAGTATGATATCATTCAATATGTTTTGTAGGATGTTGTTGGGCATTTTCAGTCGTGGCTGCTGTAGAAGGTGCTGTGAAAATCAAGACTGGTGAGTTGATCTCATTATCTGAGCAACAATTGGTTGACTGCGATGAACAGAACAATGGGTGTGATGGAGGTAATATGGATATTGCCTTCAAATATATAATACAAACCCAAGGTATTGTTAGTGAGACAGATTATCCATATCAAGAAGGTAGTCAGACATGCCAATTAAATGATCAAATGAAATTCGCAGCTCAAATAACAAATTTTGTAGACGTACCTGCCAATGATGAACAACAACTGCTACAAGCTGTAGCACAACAACCAGTATCAGTTGGCATTGCAGTCGGTGATGAATTTCAACATTACATGGGAGATGTTTATTCAGGAACATGTGGACAATCAATGAACCATGCAGTTACTGCAGTTGGTTACGGTGTAAGTGAAGATGGAACTAAGTATTGGTTGATTAAGAATTCTTGGGGTAAAGATTGGGGTGAAGAAGGTTACATGAAGTTGTTGAGGGAAAGTGGTGAACCAGGAGGTCAATGTGGCATTGCTGCACATGCTTCTTATCCCACCATATAA
Protein sequence

>MS.gene54314.t1

MIILWACAYPIMSRTLSESSVVEAHQQWMIKYGRTYTNSSELEKRKKIFKENLEYIENFNNAGNKSYKLGLNPYSDLTSEEFIASHTGLKVSDRLSSSKMRSVAVPFNLNDDIPTNFDWRQQGAVTDVKDQGSCGCCWAFSVVAAVEGAVKIKTGELISLSEQQLVDCDEQNNGCDGGNMDIAFKYIIQTQGIVSETDYPYQEGSQTCQLNDQMKFAAQITNFVDVPANDEQQLLQAVAQQPVSVGIAVGDEFQHYMGDVYSGTCGQSMNHAVTAVGYGVSEDGTKYWLIKNSWGKDWGEEGYMKLLRESGEPGGQCGIAAHASYPTI