Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54436.t1 | GAU16518.1 | 97.2 | 425 | 12 | 0 | 1 | 425 | 46 | 470 | 2.10E-233 | 818.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54436.t1 | Q9M888 | 87.2 | 436 | 48 | 2 | 15 | 445 | 60 | 492 | 1.4e-214 | 746.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54436.t1 | A0A1S2XJF4 | 96.7 | 426 | 14 | 0 | 1 | 426 | 46 | 471 | 8.2e-232 | 812.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05640 | MS.gene54436 | 0.838618 | 2.58E-57 | -1.69E-46 |
| MS.gene05732 | MS.gene54436 | 0.813944 | 1.98E-51 | -1.69E-46 |
| MS.gene057367 | MS.gene54436 | 0.80063 | 1.32E-48 | -1.69E-46 |
| MS.gene057669 | MS.gene54436 | 0.837481 | 5.07E-57 | -1.69E-46 |
| MS.gene058827 | MS.gene54436 | 0.908218 | 2.17E-81 | -1.69E-46 |
| MS.gene058828 | MS.gene54436 | 0.889301 | 2.74E-73 | -1.69E-46 |
| MS.gene058853 | MS.gene54436 | 0.830141 | 3.47E-55 | -1.69E-46 |
| MS.gene060473 | MS.gene54436 | 0.806858 | 6.71E-50 | -1.69E-46 |
| MS.gene06093 | MS.gene54436 | 0.826687 | 2.37E-54 | -1.69E-46 |
| MS.gene061260 | MS.gene54436 | 0.806815 | 6.85E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 119 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGCACAATCTTGATTAA+AGG | 0.165518 | 7.3:+91206721 | MS.gene54436:CDS |
| CCTGCCCAACCCAGGCATTC+AGG | 0.223982 | 7.3:-91206631 | None:intergenic |
| TCATAATAGCCGTTGGATTC+TGG | 0.236868 | 7.3:-91203795 | None:intergenic |
| AGAAGCAATTGATCTGTTTA+TGG | 0.251737 | 7.3:+91205027 | MS.gene54436:CDS |
| CAATTGCTTCTTCGGGCTTT+CGG | 0.277127 | 7.3:-91205015 | None:intergenic |
| ATCAGGCTTGTTGGTGGTTC+TGG | 0.280050 | 7.3:+91202004 | None:intergenic |
| AAACATGGAGCGATTGGTTT+TGG | 0.281624 | 7.3:+91206567 | MS.gene54436:CDS |
| ACAAAATTGCGCTCATTATC+AGG | 0.285123 | 7.3:-91206209 | None:intergenic |
| CGGGGAGTGGCAGTTGTTAA+TGG | 0.316427 | 7.3:-91210691 | None:intergenic |
| AACAGATCAATTGCTTCTTC+GGG | 0.317853 | 7.3:-91205022 | None:intergenic |
| AAACAGATCAATTGCTTCTT+CGG | 0.322370 | 7.3:-91205023 | None:intergenic |
| TGACTTGACCCCTGAATGCC+TGG | 0.322527 | 7.3:+91206621 | MS.gene54436:CDS |
| ACTTCTACTGTCCTCTTCAT+TGG | 0.323741 | 7.3:+91203865 | MS.gene54436:CDS |
| CGACAAAGAGATTCTCAAAA+TGG | 0.328490 | 7.3:+91204648 | MS.gene54436:CDS |
| CTTCTACTGTCCTCTTCATT+GGG | 0.335093 | 7.3:+91203866 | MS.gene54436:CDS |
| AGATGTGATTATTGCCCTTA+AGG | 0.341934 | 7.3:+91208930 | MS.gene54436:CDS |
| TTTGTCATCATCAACCAAAA+AGG | 0.363360 | 7.3:+91206227 | MS.gene54436:CDS |
| GACCCCTGAATGCCTGGGTT+GGG | 0.363612 | 7.3:+91206627 | MS.gene54436:CDS |
| TCTGCACGTCGCTTCATGTC+AGG | 0.371322 | 7.3:-91205223 | None:intergenic |
| TGCTGGGTTGCCTAGGTTGA+GGG | 0.390764 | 7.3:+91205172 | MS.gene54436:intron |
| AATCAAGATTGTGCAAGAAA+AGG | 0.393989 | 7.3:-91206716 | None:intergenic |
| TTAAAAGAAGCATACCTTAA+GGG | 0.396817 | 7.3:-91208944 | None:intergenic |
| ATCAAGATTGTGCAAGAAAA+GGG | 0.396968 | 7.3:-91206715 | None:intergenic |
| TTGAGCTATCGTGTGGTCAT+TGG | 0.398725 | 7.3:-91206906 | None:intergenic |
| GAGGGTCTCGTCCTTGATCA+TGG | 0.404962 | 7.3:+91205190 | MS.gene54436:CDS |
| CATCCACGTGTATTGGTTGA+TGG | 0.406867 | 7.3:+91204550 | MS.gene54436:CDS |
| GAGGAGCTAGAGGGAGAGGT+TGG | 0.413313 | 7.3:-91210627 | None:intergenic |
| TCGCTTCATGTCAGGGTGTC+GGG | 0.419263 | 7.3:-91205215 | None:intergenic |
| TGAGCTATCGTGTGGTCATT+GGG | 0.419501 | 7.3:-91206905 | None:intergenic |
| CCTGAATGCCTGGGTTGGGC+AGG | 0.426320 | 7.3:+91206631 | MS.gene54436:CDS |
| CTGCCCAACCCAGGCATTCA+GGG | 0.429900 | 7.3:-91206630 | None:intergenic |
| TGACCCCTGAATGCCTGGGT+TGG | 0.430026 | 7.3:+91206626 | MS.gene54436:CDS |
| AGGAGCTAGAGGGAGAGGTT+GGG | 0.435257 | 7.3:-91210626 | None:intergenic |
| TTCAAGACTCCTGTTGTAAT+GGG | 0.436558 | 7.3:+91204616 | MS.gene54436:CDS |
| GTTCAAGACTCCTGTTGTAA+TGG | 0.453647 | 7.3:+91204615 | MS.gene54436:CDS |
| TGCTAGGACAGCGGTTGCAC+AGG | 0.460244 | 7.3:+91203819 | MS.gene54436:CDS |
| TTCTCCTCTTTGCTCTGCGA+AGG | 0.466938 | 7.3:-91206546 | None:intergenic |
| CAACTGCCACTCCCCGCTAT+GGG | 0.467027 | 7.3:+91210698 | MS.gene54436:CDS |
| GGCTCAGCACCCATTACAAC+AGG | 0.467685 | 7.3:-91204625 | None:intergenic |
| GTCGCTTCATGTCAGGGTGT+CGG | 0.468131 | 7.3:-91205216 | None:intergenic |
| CCGCTATGGGGTGTAACAAA+TGG | 0.480678 | 7.3:+91210711 | MS.gene54436:CDS |
| GATATTAAACTCACTAAAGA+TGG | 0.482698 | 7.3:+91202028 | MS.gene54436:CDS |
| ATGCCGTCCGTGATGGTTTG+AGG | 0.483324 | 7.3:+91206936 | MS.gene54436:CDS |
| ACAACTGCCACTCCCCGCTA+TGG | 0.483769 | 7.3:+91210697 | MS.gene54436:CDS |
| GAAGTTAAGAAAACTGTTCA+AGG | 0.488692 | 7.3:+91208731 | MS.gene54436:CDS |
| GACAACAGATTCATCTTCAA+TGG | 0.489255 | 7.3:-91206972 | None:intergenic |
| CATCGTTGGACATGCTTGCA+AGG | 0.489949 | 7.3:+91206261 | MS.gene54436:CDS |
| TGAAGAGAAATATACATTTG+TGG | 0.490042 | 7.3:+91206678 | MS.gene54436:CDS |
| TGCCGTCCGTGATGGTTTGA+GGG | 0.490496 | 7.3:+91206937 | MS.gene54436:CDS |
| TATGAATGAATCAGGCTTGT+TGG | 0.499941 | 7.3:+91201995 | None:intergenic |
| CATTTGTTACACCCCATAGC+GGG | 0.500966 | 7.3:-91210710 | None:intergenic |
| GAGGAGAAACATGGAGCGAT+TGG | 0.503797 | 7.3:+91206561 | MS.gene54436:CDS |
| TTGAAACAGCGTGCACAACT+TGG | 0.505401 | 7.3:+91208835 | MS.gene54436:intron |
| CTGCACGTCGCTTCATGTCA+GGG | 0.505991 | 7.3:-91205222 | None:intergenic |
| AGGAGAGGAGCTAGAGGGAG+AGG | 0.508815 | 7.3:-91210631 | None:intergenic |
| TCTCCTCTTTGCTCTGCGAA+GGG | 0.509155 | 7.3:-91206545 | None:intergenic |
| AAGACACTTGCTGAAAACTC+TGG | 0.514511 | 7.3:+91208895 | MS.gene54436:CDS |
| GGTTGTAGCTGAAAGGCGTC+AGG | 0.521576 | 7.3:+91206008 | MS.gene54436:CDS |
| AATCATTGAACTGAAAAGAA+AGG | 0.521841 | 7.3:+91206050 | MS.gene54436:CDS |
| GACTTGACCCCTGAATGCCT+GGG | 0.524632 | 7.3:+91206622 | MS.gene54436:CDS |
| TGCTGAACAGAGAGAGGCTA+TGG | 0.524727 | 7.3:+91205987 | MS.gene54436:CDS |
| AGGTATGCATCCACGTGTAT+TGG | 0.529801 | 7.3:+91204543 | MS.gene54436:intron |
| ATTTGATGTTGACACACGCT+TGG | 0.538547 | 7.3:+91205075 | MS.gene54436:CDS |
| ACGTCACTGCACATTTGCCT+TGG | 0.542654 | 7.3:+91210588 | MS.gene54436:CDS |
| TTGCAAGCATGTCCAACGAT+GGG | 0.544621 | 7.3:-91206259 | None:intergenic |
| AAAATCCACCTTTGTCCTCA+CGG | 0.544703 | 7.3:-91204683 | None:intergenic |
| TATCTCTTGAGTATGAAAAG+AGG | 0.548265 | 7.3:+91205272 | MS.gene54436:CDS |
| TTACACCCCATAGCGGGGAG+TGG | 0.549250 | 7.3:-91210704 | None:intergenic |
| CAACGGCTATTATGATTGCT+AGG | 0.550728 | 7.3:+91203803 | MS.gene54436:CDS |
| ATTAAAGATGCCGTCCGTGA+TGG | 0.553606 | 7.3:+91206929 | MS.gene54436:CDS |
| CAGAGCAAAGAGGAGAAACA+TGG | 0.556365 | 7.3:+91206552 | MS.gene54436:CDS |
| TGAAGATGAATCTGTTGTCT+TGG | 0.560777 | 7.3:+91206976 | MS.gene54436:CDS |
| TATTATGATTGCTAGGACAG+CGG | 0.565083 | 7.3:+91203810 | MS.gene54436:CDS |
| CTAGCAATCATAATAGCCGT+TGG | 0.568498 | 7.3:-91203802 | None:intergenic |
| TGTGAGTTCGCCGATGATGA+TGG | 0.571262 | 7.3:-91210661 | None:intergenic |
| AGGCTATGGTTGTAGCTGAA+AGG | 0.577063 | 7.3:+91206001 | MS.gene54436:CDS |
| ATGCAGAGGAGAGGAGCTAG+AGG | 0.577319 | 7.3:-91210637 | None:intergenic |
| GTTGCACAGGATGACATTAG+TGG | 0.577599 | 7.3:+91203832 | MS.gene54436:CDS |
| GGTCACAATCTTCCTTGCCA+AGG | 0.579635 | 7.3:-91210605 | None:intergenic |
| CTTTAATTTGAGCTATCGTG+TGG | 0.584126 | 7.3:-91206913 | None:intergenic |
| TCTGAGCGTTACATCGATGA+AGG | 0.585917 | 7.3:+91203904 | MS.gene54436:CDS |
| GAATGAATCAGGCTTGTTGG+TGG | 0.588106 | 7.3:+91201998 | None:intergenic |
| CAGGATGACATTAGTGGTGA+TGG | 0.588583 | 7.3:+91203838 | MS.gene54436:CDS |
| CTCAAATGCTGAACAGAGAG+AGG | 0.590341 | 7.3:+91205981 | MS.gene54436:CDS |
| CTTGCAAGCATGTCCAACGA+TGG | 0.590353 | 7.3:-91206260 | None:intergenic |
| ACTGCACATTTGCCTTGGCA+AGG | 0.590609 | 7.3:+91210593 | MS.gene54436:CDS |
| CTTCTCAAGAAATTGAAGAG+TGG | 0.591866 | 7.3:-91204593 | None:intergenic |
| GTTTACAGCCTCACCTCCAC+AGG | 0.594743 | 7.3:-91206590 | None:intergenic |
| GGTGTCGGGAACCATGATCA+AGG | 0.594859 | 7.3:-91205201 | None:intergenic |
| TCATAAGTTCCCCAATGAAG+AGG | 0.596505 | 7.3:-91203876 | None:intergenic |
| TTGGACATGCTTGCAAGGGA+AGG | 0.597063 | 7.3:+91206266 | MS.gene54436:CDS |
| AAGTTAAGAAAACTGTTCAA+GGG | 0.601310 | 7.3:+91208732 | MS.gene54436:CDS |
| TTCTACTGTCCTCTTCATTG+GGG | 0.604196 | 7.3:+91203867 | MS.gene54436:CDS |
| CCTCTGCATGCCATCATCAT+CGG | 0.605026 | 7.3:+91210651 | MS.gene54436:CDS |
| ATCGTTGGACATGCTTGCAA+GGG | 0.605046 | 7.3:+91206262 | MS.gene54436:CDS |
| TGCAAGCATGTCCAACGATG+GGG | 0.606757 | 7.3:-91206258 | None:intergenic |
| TGCCCAACCCAGGCATTCAG+GGG | 0.607667 | 7.3:-91206629 | None:intergenic |
| TGCCCTCAAACCATCACGGA+CGG | 0.608668 | 7.3:-91206939 | None:intergenic |
| GACTAATATAATTGTTGATG+CGG | 0.612152 | 7.3:+91204846 | MS.gene54436:CDS |
| TTAGTGTATGAACATGTCCT+TGG | 0.624398 | 7.3:+91206655 | MS.gene54436:CDS |
| GATGATGGCATGCAGAGGAG+AGG | 0.626248 | 7.3:-91210646 | None:intergenic |
| GCGTGCACAACTTGGTGTTG+AGG | 0.626670 | 7.3:+91208843 | MS.gene54436:intron |
| AGTTAAGAAAACTGTTCAAG+GGG | 0.628362 | 7.3:+91208733 | MS.gene54436:CDS |
| ACACTAATCCTGCCCAACCC+AGG | 0.628728 | 7.3:-91206639 | None:intergenic |
| AACTGCCACTCCCCGCTATG+GGG | 0.629360 | 7.3:+91210699 | MS.gene54436:CDS |
| AAGAACAACCGTGAGGACAA+AGG | 0.629455 | 7.3:+91204675 | MS.gene54436:CDS |
| TGGTCGAGATTATGCACATG+CGG | 0.635900 | 7.3:+91205047 | MS.gene54436:CDS |
| CCGATGATGATGGCATGCAG+AGG | 0.641612 | 7.3:-91210651 | None:intergenic |
| GCAAGCATGTCCAACGATGG+GGG | 0.653147 | 7.3:-91206257 | None:intergenic |
| TCACTGCCCTCAAACCATCA+CGG | 0.655296 | 7.3:-91206943 | None:intergenic |
| ATTTGTTACACCCCATAGCG+GGG | 0.656660 | 7.3:-91210709 | None:intergenic |
| TGCAGAGGAGAGGAGCTAGA+GGG | 0.657650 | 7.3:-91210636 | None:intergenic |
| TTGCCCTTCGCAGAGCAAAG+AGG | 0.664074 | 7.3:+91206542 | MS.gene54436:CDS |
| TCAGCAAATCCAGAATCCAA+CGG | 0.674693 | 7.3:+91203786 | MS.gene54436:intron |
| ATGTATATTTCTCTTCACCA+AGG | 0.675861 | 7.3:-91206672 | None:intergenic |
| AATCCATCAACCAATACACG+TGG | 0.689768 | 7.3:-91204553 | None:intergenic |
| CCATTTGTTACACCCCATAG+CGG | 0.695241 | 7.3:-91210711 | None:intergenic |
| AAGGCAAATGTGCAGTGACG+TGG | 0.700608 | 7.3:-91210586 | None:intergenic |
| TGGTAGCAAGAACAACCGTG+AGG | 0.779725 | 7.3:+91204668 | MS.gene54436:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 91202009 | 91210746 | 91202009 | ID=MS.gene54436 |
| chr7.3 | mRNA | 91202009 | 91210746 | 91202009 | ID=MS.gene54436.t1;Parent=MS.gene54436 |
| chr7.3 | exon | 91202009 | 91202072 | 91202009 | ID=MS.gene54436.t1.exon1;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91202009 | 91202072 | 91202009 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91203790 | 91203925 | 91203790 | ID=MS.gene54436.t1.exon2;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91203790 | 91203925 | 91203790 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91204545 | 91204696 | 91204545 | ID=MS.gene54436.t1.exon3;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91204545 | 91204696 | 91204545 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91204820 | 91204867 | 91204820 | ID=MS.gene54436.t1.exon4;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91204820 | 91204867 | 91204820 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91205004 | 91205096 | 91205004 | ID=MS.gene54436.t1.exon5;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91205004 | 91205096 | 91205004 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91205187 | 91205293 | 91205187 | ID=MS.gene54436.t1.exon6;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91205187 | 91205293 | 91205187 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91205957 | 91206071 | 91205957 | ID=MS.gene54436.t1.exon7;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91205957 | 91206071 | 91205957 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91206203 | 91206289 | 91206203 | ID=MS.gene54436.t1.exon8;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91206203 | 91206289 | 91206203 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91206538 | 91206742 | 91206538 | ID=MS.gene54436.t1.exon9;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91206538 | 91206742 | 91206538 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91206903 | 91206997 | 91206903 | ID=MS.gene54436.t1.exon10;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91206903 | 91206997 | 91206903 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91208686 | 91208754 | 91208686 | ID=MS.gene54436.t1.exon11;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91208686 | 91208754 | 91208686 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91208844 | 91208951 | 91208844 | ID=MS.gene54436.t1.exon12;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91208844 | 91208951 | 91208844 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| chr7.3 | exon | 91210582 | 91210746 | 91210582 | ID=MS.gene54436.t1.exon13;Parent=MS.gene54436.t1 |
| chr7.3 | CDS | 91210582 | 91210746 | 91210582 | ID=cds.MS.gene54436.t1;Parent=MS.gene54436.t1 |
| Gene Sequence |
| Protein sequence |
