Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058828.t1 | XP_003629912.1 | 99.8 | 544 | 1 | 0 | 1 | 544 | 1 | 544 | 2.90E-292 | 1013.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058828.t1 | P28769 | 90.6 | 544 | 51 | 0 | 1 | 544 | 1 | 544 | 4.6e-270 | 931.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058828.t1 | G7L831 | 99.8 | 544 | 1 | 0 | 1 | 544 | 1 | 544 | 2.1e-292 | 1013.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051629 | MS.gene058828 | 0.805263 | 1.45E-49 | -1.69E-46 |
| MS.gene052367 | MS.gene058828 | 0.816474 | 5.43E-52 | -1.69E-46 |
| MS.gene053348 | MS.gene058828 | 0.810345 | 1.21E-50 | -1.69E-46 |
| MS.gene054633 | MS.gene058828 | 0.801546 | 8.56E-49 | -1.69E-46 |
| MS.gene056041 | MS.gene058828 | 0.804954 | 1.69E-49 | -1.69E-46 |
| MS.gene05640 | MS.gene058828 | 0.800453 | 1.43E-48 | -1.69E-46 |
| MS.gene056526 | MS.gene058828 | 0.806569 | 7.72E-50 | -1.69E-46 |
| MS.gene058827 | MS.gene058828 | 0.978871 | 9.66E-147 | -1.69E-46 |
| MS.gene058828 | MS.gene060473 | 0.846755 | 1.78E-59 | -1.69E-46 |
| MS.gene058828 | MS.gene060565 | 0.834567 | 2.78E-56 | -1.69E-46 |
| MS.gene058828 | MS.gene06093 | 0.812285 | 4.58E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene061260 | 0.819111 | 1.38E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene061275 | 0.812136 | 4.93E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene061357 | 0.816994 | 4.15E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene063035 | 0.814162 | 1.77E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene063037 | 0.805907 | 1.06E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene066265 | 0.805602 | 1.23E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene069090 | 0.824087 | 9.76E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene069339 | 0.859193 | 4.90E-63 | -1.69E-46 |
| MS.gene058828 | MS.gene074451 | 0.827821 | 1.27E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene08420 | 0.801447 | 8.96E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene08836 | 0.813476 | 2.51E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene08988 | 0.805512 | 1.29E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene09177 | 0.805688 | 1.18E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene09178 | 0.816031 | 6.82E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene09258 | 0.816035 | 6.81E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene09540 | 0.804529 | 2.07E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene09543 | 0.802619 | 5.15E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene09594 | 0.818421 | 1.98E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene09808 | 0.82674 | 2.30E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene20224 | 0.824574 | 7.50E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene21320 | 0.813929 | 2.00E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene21888 | 0.800677 | 1.29E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene22383 | 0.81675 | 4.71E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene22464 | 0.808677 | 2.75E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene23323 | 0.829048 | 6.40E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene24543 | 0.829317 | 5.51E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene26114 | 0.84183 | 3.75E-58 | -1.69E-46 |
| MS.gene058828 | MS.gene26619 | 0.802146 | 6.44E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene26706 | 0.801955 | 7.05E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene26803 | 0.812322 | 4.49E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene27811 | 0.815178 | 1.06E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene27812 | 0.819924 | 9.00E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene27902 | 0.982758 | 6.35E-156 | -1.69E-46 |
| MS.gene058828 | MS.gene27941 | 0.814382 | 1.59E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene28867 | 0.812428 | 4.26E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene32514 | 0.809306 | 2.02E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene32638 | 0.812353 | 4.42E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene32975 | 0.833923 | 4.04E-56 | -1.69E-46 |
| MS.gene058828 | MS.gene35150 | 0.820778 | 5.73E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene35668 | 0.81594 | 7.14E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene36485 | 0.820417 | 6.94E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene37925 | 0.81315 | 2.96E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene38724 | 0.800517 | 1.39E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene40335 | 0.805102 | 1.57E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene40405 | 0.812974 | 3.24E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene41273 | 0.83122 | 1.89E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene42910 | 0.810527 | 1.10E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene44100 | 0.800835 | 1.20E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene44251 | 0.800631 | 1.32E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene47899 | 0.800844 | 1.19E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene48067 | 0.826585 | 2.50E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene49165 | 0.812814 | 3.51E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene49312 | 0.808345 | 3.24E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene49744 | 0.818007 | 2.45E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene50484 | 0.822442 | 2.36E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene50613 | 0.80389 | 2.81E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene50736 | 0.812292 | 4.56E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene53408 | 0.81885 | 1.58E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene54436 | 0.889301 | 2.74E-73 | -1.69E-46 |
| MS.gene058828 | MS.gene55817 | 0.809148 | 2.18E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene55819 | 0.800792 | 1.22E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene57219 | 0.816317 | 5.89E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene57751 | 0.80998 | 1.45E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene58234 | 0.809988 | 1.44E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene58754 | 0.801957 | 7.05E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene58835 | 0.806291 | 8.83E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene59357 | 0.83045 | 2.92E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene61977 | 0.826864 | 2.15E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene64538 | 0.812949 | 3.28E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene65301 | 0.800831 | 1.20E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene68361 | 0.801776 | 7.68E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene70141 | 0.800452 | 1.43E-48 | -1.69E-46 |
| MS.gene058828 | MS.gene70403 | 0.825103 | 5.62E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene71431 | 0.819894 | 9.14E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene72708 | 0.819085 | 1.40E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene74232 | 0.817388 | 3.38E-52 | -1.69E-46 |
| MS.gene058828 | MS.gene74328 | 0.8233 | 1.49E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene75642 | 0.839767 | 1.30E-57 | -1.69E-46 |
| MS.gene058828 | MS.gene77563 | 0.830123 | 3.51E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene78741 | 0.829619 | 4.65E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene79251 | 0.823965 | 1.04E-53 | -1.69E-46 |
| MS.gene058828 | MS.gene79921 | 0.839064 | 1.98E-57 | -1.69E-46 |
| MS.gene058828 | MS.gene80994 | 0.84251 | 2.47E-58 | -1.69E-46 |
| MS.gene058828 | MS.gene91781 | 0.813723 | 2.22E-51 | -1.69E-46 |
| MS.gene058828 | MS.gene93102 | 0.80168 | 8.03E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene96186 | 0.804744 | 1.86E-49 | -1.69E-46 |
| MS.gene058828 | MS.gene96390 | 0.824723 | 6.92E-54 | -1.69E-46 |
| MS.gene058828 | MS.gene97876 | 0.836795 | 7.59E-57 | -1.69E-46 |
| MS.gene058828 | MS.gene99337 | 0.842063 | 3.25E-58 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058828.t1 | MTR_8g088270 | 99.816 | 544 | 1 | 0 | 1 | 544 | 1 | 544 | 0.0 | 1097 |
| MS.gene058828.t1 | MTR_8g010750 | 70.025 | 407 | 26 | 8 | 21 | 376 | 30 | 391 | 8.46e-175 | 503 |
| MS.gene058828.t1 | MTR_5g087560 | 37.665 | 531 | 305 | 8 | 17 | 540 | 21 | 532 | 2.87e-110 | 340 |
| MS.gene058828.t1 | MTR_7g113470 | 35.120 | 541 | 319 | 10 | 1 | 534 | 1 | 516 | 1.49e-91 | 290 |
| MS.gene058828.t1 | MTR_7g067470 | 35.104 | 527 | 311 | 9 | 15 | 534 | 14 | 516 | 2.97e-90 | 287 |
| MS.gene058828.t1 | MTR_3g109925 | 34.286 | 525 | 317 | 9 | 19 | 534 | 24 | 529 | 3.84e-81 | 263 |
| MS.gene058828.t1 | MTR_3g086330 | 32.707 | 532 | 329 | 10 | 11 | 534 | 20 | 530 | 9.00e-73 | 241 |
| MS.gene058828.t1 | MTR_2g013540 | 30.697 | 531 | 347 | 8 | 13 | 538 | 13 | 527 | 2.90e-71 | 238 |
| MS.gene058828.t1 | MTR_1g030650 | 30.174 | 517 | 335 | 9 | 23 | 534 | 28 | 523 | 2.16e-66 | 224 |
| MS.gene058828.t1 | MTR_3g109925 | 31.309 | 527 | 273 | 10 | 19 | 534 | 24 | 472 | 5.79e-64 | 216 |
| MS.gene058828.t1 | MTR_6g032870 | 29.400 | 517 | 339 | 9 | 23 | 534 | 25 | 520 | 4.53e-63 | 216 |
| MS.gene058828.t1 | MTR_7g406870 | 26.857 | 525 | 351 | 8 | 24 | 535 | 22 | 526 | 4.57e-56 | 197 |
| MS.gene058828.t1 | MTR_7g406870 | 24.654 | 434 | 294 | 8 | 115 | 535 | 1 | 414 | 4.92e-34 | 134 |
| MS.gene058828.t1 | MTR_2g013540 | 26.463 | 393 | 269 | 7 | 151 | 538 | 1 | 378 | 1.57e-31 | 126 |
| MS.gene058828.t1 | MTR_8g105970 | 27.931 | 290 | 150 | 7 | 225 | 500 | 109 | 353 | 7.53e-18 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058828.t1 | AT3G20050 | 90.625 | 544 | 51 | 0 | 1 | 544 | 1 | 544 | 0.0 | 1006 |
| MS.gene058828.t1 | AT3G11830 | 37.023 | 524 | 304 | 6 | 24 | 540 | 28 | 532 | 1.27e-103 | 323 |
| MS.gene058828.t1 | AT3G11830 | 37.023 | 524 | 302 | 7 | 24 | 540 | 28 | 530 | 2.59e-102 | 319 |
| MS.gene058828.t1 | AT3G18190 | 35.606 | 528 | 308 | 11 | 18 | 534 | 27 | 533 | 2.14e-88 | 282 |
| MS.gene058828.t1 | AT5G20890 | 34.594 | 529 | 311 | 11 | 15 | 534 | 14 | 516 | 1.61e-82 | 267 |
| MS.gene058828.t1 | AT1G24510 | 32.197 | 528 | 331 | 7 | 14 | 534 | 23 | 530 | 3.13e-73 | 243 |
| MS.gene058828.t1 | AT5G26360 | 30.624 | 529 | 350 | 6 | 13 | 538 | 13 | 527 | 9.21e-72 | 239 |
| MS.gene058828.t1 | AT3G03960 | 29.594 | 517 | 338 | 9 | 23 | 534 | 31 | 526 | 2.37e-64 | 219 |
| MS.gene058828.t1 | AT3G02530 | 28.681 | 523 | 338 | 9 | 27 | 535 | 25 | 526 | 3.83e-61 | 211 |
| MS.gene058828.t1 | AT5G16070 | 28.269 | 520 | 343 | 8 | 27 | 535 | 25 | 525 | 7.83e-60 | 207 |
| MS.gene058828.t1 | AT1G24510 | 30.247 | 486 | 312 | 7 | 56 | 534 | 12 | 477 | 2.05e-58 | 202 |
| MS.gene058828.t1 | AT1G24510 | 29.536 | 474 | 307 | 7 | 68 | 534 | 1 | 454 | 5.59e-53 | 187 |
| MS.gene058828.t1 | AT5G56500 | 22.939 | 558 | 329 | 20 | 31 | 539 | 74 | 579 | 1.27e-11 | 67.8 |
| MS.gene058828.t1 | AT5G56500 | 22.939 | 558 | 329 | 20 | 31 | 539 | 74 | 579 | 1.27e-11 | 67.8 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGCATTATCAGTTTATT+TGG | 0.170818 | 4.3:+12924991 | MS.gene058828:CDS |
| CAGTGATTTCTTTGCTAATT+TGG | 0.196704 | 4.3:+12919322 | MS.gene058828:CDS |
| GAATACCTTGCTACAACTTT+AGG | 0.217589 | 4.3:+12925013 | MS.gene058828:CDS |
| CTGCCCAAGGAATGCCTCTT+AGG | 0.219812 | 4.3:+12921004 | MS.gene058828:CDS |
| GATGCTGTAATCATGATTAA+AGG | 0.288628 | 4.3:+12922896 | MS.gene058828:CDS |
| ATTCTACTACCAACCTTGAT+TGG | 0.298920 | 4.3:-12920738 | None:intergenic |
| GGTTGAACTTGCTGAACTTC+AGG | 0.305715 | 4.3:+12918165 | MS.gene058828:CDS |
| GGTCTCAACATTTGCTGATA+TGG | 0.306308 | 4.3:+12922802 | MS.gene058828:intron |
| CTATCTTTCCAGTACGGGTT+TGG | 0.307511 | 4.3:+12925911 | MS.gene058828:intron |
| ACTTACTGCACTTGAAGTTT+TGG | 0.311156 | 4.3:-12922921 | None:intergenic |
| GAGCAAAGTCAAAATTATTC+AGG | 0.342350 | 4.3:+12925992 | MS.gene058828:CDS |
| CAATGTTGCACCGGTTGCTT+TGG | 0.351517 | 4.3:-12922288 | None:intergenic |
| CTTTGAGGACATGCTGGTCT+TGG | 0.352985 | 4.3:-12919285 | None:intergenic |
| CAGACCTGGTTGCTAAATTA+CGG | 0.358667 | 4.3:+12925467 | MS.gene058828:CDS |
| AATTGATGACATGGCGCTTA+AGG | 0.368284 | 4.3:+12921511 | MS.gene058828:CDS |
| AATGGTTATGCTCTTAATAC+TGG | 0.369065 | 4.3:+12920976 | MS.gene058828:CDS |
| CAGATGCTTGTTGATGATAT+CGG | 0.399758 | 4.3:+12917982 | MS.gene058828:intron |
| TTTCAGTACTTTGTTGAGGC+TGG | 0.410605 | 4.3:+12922218 | MS.gene058828:intron |
| AACCAGGTCTGTGGCATCCT+TGG | 0.425381 | 4.3:-12925455 | None:intergenic |
| TTCTACTACCAACCTTGATT+GGG | 0.427473 | 4.3:-12920737 | None:intergenic |
| ATTCAGGTGGTGGCTGGTGG+AGG | 0.433679 | 4.3:+12924959 | MS.gene058828:intron |
| CTTGAGAAATCTTACCTTGA+CGG | 0.434983 | 4.3:-12918551 | None:intergenic |
| AGAGAGCTAATGATCTTGTT+AGG | 0.440419 | 4.3:+12918322 | MS.gene058828:intron |
| TGCGGACGTCTTGGCCGGAT+TGG | 0.445829 | 4.3:-12917703 | None:intergenic |
| CGTCATGTTGCCAAAGCAAC+CGG | 0.451920 | 4.3:+12922278 | MS.gene058828:CDS |
| AATGTTGTTCTGACTACTAA+AGG | 0.453936 | 4.3:+12921488 | MS.gene058828:CDS |
| CACTTGTAGGTTGAGAAGCT+TGG | 0.454077 | 4.3:+12919239 | MS.gene058828:intron |
| GAACTTCAGGATAGAGAAGT+TGG | 0.456067 | 4.3:+12918178 | MS.gene058828:CDS |
| AAGTATTAGTTTCTGATCCC+AGG | 0.456823 | 4.3:+12921094 | MS.gene058828:CDS |
| AGACCTGGTTGCTAAATTAC+GGG | 0.457737 | 4.3:+12925468 | MS.gene058828:CDS |
| ATGCTGTAATCATGATTAAA+GGG | 0.460572 | 4.3:+12922897 | MS.gene058828:CDS |
| TGTAAAGATGACTAACCAAC+GGG | 0.463714 | 4.3:+12920701 | MS.gene058828:CDS |
| GTCACCAGCTATCAGCTTTG+AGG | 0.466133 | 4.3:-12919300 | None:intergenic |
| TGGCGTTCGCCGGAGATGTC+AGG | 0.467083 | 4.3:-12917683 | None:intergenic |
| TTGAGTGCGGACGTCTTGGC+CGG | 0.473398 | 4.3:-12917708 | None:intergenic |
| ATCTCCGGCGAACGCCAATC+CGG | 0.474311 | 4.3:+12917689 | MS.gene058828:CDS |
| TGTACCTTGTCGAGACCAAC+GGG | 0.479862 | 4.3:-12917879 | None:intergenic |
| AGTTTGTGCAGCATAGTGGC+AGG | 0.480386 | 4.3:-12925491 | None:intergenic |
| ATACCCTAAGAGGCATTCCT+TGG | 0.482129 | 4.3:-12921008 | None:intergenic |
| ACTAGAAACAACTTGGAAGC+TGG | 0.485060 | 4.3:+12925951 | MS.gene058828:CDS |
| GCTTGTCAAGGATGAAGGTC+AGG | 0.491199 | 4.3:+12926160 | MS.gene058828:CDS |
| AGACGAAAAGCTTGCCGTCA+AGG | 0.498910 | 4.3:+12918537 | MS.gene058828:CDS |
| GCTTGATCATATCGTCAATC+CGG | 0.503371 | 4.3:-12926139 | None:intergenic |
| TGCCCAAGGAATGCCTCTTA+GGG | 0.506306 | 4.3:+12921005 | MS.gene058828:CDS |
| TCAGGGAATCAATATATTGA+AGG | 0.506625 | 4.3:+12920918 | MS.gene058828:intron |
| TGCTACAACTTTAGGATCGC+GGG | 0.508460 | 4.3:+12925021 | MS.gene058828:CDS |
| CAAAGCAACCGGTGCAACAT+TGG | 0.508709 | 4.3:+12922289 | MS.gene058828:CDS |
| ATCAAGCTTGTCAAGGATGA+AGG | 0.509988 | 4.3:+12926155 | MS.gene058828:CDS |
| TCTATATCCACTGATAATTG+AGG | 0.510888 | 4.3:-12918361 | None:intergenic |
| TTTAGGATCGCGGGAGCAGT+TGG | 0.511859 | 4.3:+12925030 | MS.gene058828:CDS |
| AAGCTGCTATTACTATTCTC+CGG | 0.516464 | 4.3:+12926120 | MS.gene058828:CDS |
| TTGGCCGGATTGGCGTTCGC+CGG | 0.521185 | 4.3:-12917693 | None:intergenic |
| TGCTACTATACTTAAGATGC+TGG | 0.521772 | 4.3:+12918029 | MS.gene058828:CDS |
| TACCCTAAGAGGCATTCCTT+GGG | 0.521776 | 4.3:-12921007 | None:intergenic |
| TGACTACATGCTTGATGAGA+TGG | 0.525127 | 4.3:+12923778 | MS.gene058828:CDS |
| ATGTAAATTCAGGTGGTGGC+TGG | 0.525195 | 4.3:+12924953 | MS.gene058828:intron |
| AGTTGAACATCCCGCTGCTA+AGG | 0.529381 | 4.3:+12918053 | MS.gene058828:CDS |
| CTTTAGTTTGTGCAGCATAG+TGG | 0.535439 | 4.3:-12925495 | None:intergenic |
| AGTATTAGTTTCTGATCCCA+GGG | 0.537249 | 4.3:+12921095 | MS.gene058828:CDS |
| CAGAGAGGCTGACATGACTA+AGG | 0.538129 | 4.3:+12921433 | MS.gene058828:intron |
| ATGTCCTCAAAGCTGATAGC+TGG | 0.539484 | 4.3:+12919296 | MS.gene058828:CDS |
| ATCGAGAAATTACTGAAAGC+TGG | 0.540178 | 4.3:+12921461 | MS.gene058828:CDS |
| AATCAAACTCCTGACATCTC+CGG | 0.541969 | 4.3:+12917674 | MS.gene058828:CDS |
| TTTGCAGGGGATACCCTAAG+AGG | 0.545005 | 4.3:-12921018 | None:intergenic |
| CAAGACGTCCGCACTCAAAA+CGG | 0.547818 | 4.3:+12917713 | MS.gene058828:CDS |
| TAAATTCAGGTGGTGGCTGG+TGG | 0.548875 | 4.3:+12924956 | MS.gene058828:intron |
| TATCAGCTTTGAGGACATGC+TGG | 0.550203 | 4.3:-12919291 | None:intergenic |
| AGGAAAGACTAGAAACAACT+TGG | 0.552501 | 4.3:+12925944 | MS.gene058828:CDS |
| CAGATAAGAAGCATTTATCA+AGG | 0.555568 | 4.3:+12925518 | MS.gene058828:CDS |
| ATTAGATTAACCTTAGCAGC+GGG | 0.562482 | 4.3:-12918063 | None:intergenic |
| TCAACATTTGCTGATATGGA+AGG | 0.562540 | 4.3:+12922806 | MS.gene058828:CDS |
| TTGTACCTTGTCGAGACCAA+CGG | 0.566679 | 4.3:-12917880 | None:intergenic |
| CTGGTTTCAGTACTTTGTTG+AGG | 0.569526 | 4.3:+12922214 | MS.gene058828:intron |
| AACATTTGCTGATATGGAAG+GGG | 0.570131 | 4.3:+12922808 | MS.gene058828:CDS |
| ACGGGTTTGGATCTTTCAGA+AGG | 0.572129 | 4.3:+12925924 | MS.gene058828:CDS |
| TATCCACTGATAATTGAGGT+TGG | 0.573056 | 4.3:-12918357 | None:intergenic |
| ATTTCTTGGAACTGCAGATG+AGG | 0.576660 | 4.3:+12922850 | MS.gene058828:CDS |
| GTCTCTGTTAATCATTCCAA+AGG | 0.580186 | 4.3:+12925072 | MS.gene058828:CDS |
| AATACTGGACGTGCTGCCCA+AGG | 0.580408 | 4.3:+12920991 | MS.gene058828:CDS |
| CAACATTTGCTGATATGGAA+GGG | 0.582382 | 4.3:+12922807 | MS.gene058828:CDS |
| GGTATCTGACTTCACCCCGT+TGG | 0.587784 | 4.3:-12920716 | None:intergenic |
| GCTTGCTGTCAATGCTGCCA+AGG | 0.590241 | 4.3:+12925438 | MS.gene058828:CDS |
| AACTGCAGATGAGGTTGTTG+AGG | 0.595904 | 4.3:+12922859 | MS.gene058828:CDS |
| TAGGTCTCGTTAATTCTCAG+AGG | 0.599649 | 4.3:+12923749 | MS.gene058828:intron |
| AAAACCTCACTCGGTCCCGT+TGG | 0.602554 | 4.3:+12917864 | MS.gene058828:CDS |
| TGAAGTCAGATACCCAATCA+AGG | 0.604042 | 4.3:+12920725 | MS.gene058828:CDS |
| ATTTGCTGATATGGAAGGGG+AGG | 0.604182 | 4.3:+12922811 | MS.gene058828:CDS |
| TGCCAAGGATGCCACAGACC+TGG | 0.605336 | 4.3:+12925453 | MS.gene058828:CDS |
| CGATATGATCAAGCTTGTCA+AGG | 0.608330 | 4.3:+12926148 | MS.gene058828:CDS |
| GATGTGACTATTACTAATGA+TGG | 0.618021 | 4.3:+12918006 | MS.gene058828:CDS |
| AAGGCATACCAATGTTGCAC+CGG | 0.620442 | 4.3:-12922297 | None:intergenic |
| GAGACCAACGGGACCGAGTG+AGG | 0.622750 | 4.3:-12917868 | None:intergenic |
| CGGTCCCGTTGGTCTCGACA+AGG | 0.624347 | 4.3:+12917875 | MS.gene058828:CDS |
| AACTCGAGAAAATTCGCCAA+AGG | 0.625053 | 4.3:+12921118 | MS.gene058828:CDS |
| TTGCTACAACTTTAGGATCG+CGG | 0.632065 | 4.3:+12925020 | MS.gene058828:CDS |
| TGGAATGATTAACAGAGACT+CGG | 0.632335 | 4.3:-12925068 | None:intergenic |
| CTGTAAAGATGACTAACCAA+CGG | 0.633990 | 4.3:+12920700 | MS.gene058828:CDS |
| TAATTTAGCAACCAGGTCTG+TGG | 0.634341 | 4.3:-12925464 | None:intergenic |
| AATTAGATTAACCTTAGCAG+CGG | 0.635062 | 4.3:-12918064 | None:intergenic |
| AGGCCCGTAATTTAGCAACC+AGG | 0.638171 | 4.3:-12925471 | None:intergenic |
| CATCCAACCTCAATTATCAG+TGG | 0.640722 | 4.3:+12918354 | MS.gene058828:CDS |
| TCTGCAGTTCCAAGAAATGA+CGG | 0.640766 | 4.3:-12922845 | None:intergenic |
| AACATCGTGAAAACCTCACT+CGG | 0.643474 | 4.3:+12917855 | MS.gene058828:CDS |
| ATCAATATATTGAAGGCACA+TGG | 0.646632 | 4.3:+12920925 | MS.gene058828:CDS |
| TACTAAAGGAATTGATGACA+TGG | 0.657873 | 4.3:+12921502 | MS.gene058828:CDS |
| GCGATCCTAAAGTTGTAGCA+AGG | 0.663247 | 4.3:-12925018 | None:intergenic |
| CAGGATAGAGAAGTTGGAGA+CGG | 0.685651 | 4.3:+12918184 | MS.gene058828:CDS |
| CAATTGCTGTGAGACGTGTG+CGG | 0.806188 | 4.3:+12922243 | MS.gene058828:CDS |
| GTAAAGATGACTAACCAACG+GGG | 0.853539 | 4.3:+12920702 | MS.gene058828:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 12917662 | 12926190 | 12917662 | ID=MS.gene058828 |
| chr4.3 | mRNA | 12917662 | 12926190 | 12917662 | ID=MS.gene058828.t1;Parent=MS.gene058828 |
| chr4.3 | exon | 12917662 | 12917734 | 12917662 | ID=MS.gene058828.t1.exon1;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12917662 | 12917734 | 12917662 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12917832 | 12917896 | 12917832 | ID=MS.gene058828.t1.exon2;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12917832 | 12917896 | 12917832 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12917985 | 12918074 | 12917985 | ID=MS.gene058828.t1.exon3;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12917985 | 12918074 | 12917985 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12918160 | 12918246 | 12918160 | ID=MS.gene058828.t1.exon4;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12918160 | 12918246 | 12918160 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12918324 | 12918383 | 12918324 | ID=MS.gene058828.t1.exon5;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12918324 | 12918383 | 12918324 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12918508 | 12918558 | 12918508 | ID=MS.gene058828.t1.exon6;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12918508 | 12918558 | 12918508 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12919248 | 12919343 | 12919248 | ID=MS.gene058828.t1.exon7;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12919248 | 12919343 | 12919248 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12920681 | 12920746 | 12920681 | ID=MS.gene058828.t1.exon8;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12920681 | 12920746 | 12920681 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12920922 | 12921139 | 12920922 | ID=MS.gene058828.t1.exon9;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12920922 | 12921139 | 12920922 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12921436 | 12921532 | 12921436 | ID=MS.gene058828.t1.exon10;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12921436 | 12921532 | 12921436 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12922224 | 12922310 | 12922224 | ID=MS.gene058828.t1.exon11;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12922224 | 12922310 | 12922224 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12922803 | 12922937 | 12922803 | ID=MS.gene058828.t1.exon12;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12922803 | 12922937 | 12922803 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12923752 | 12923856 | 12923752 | ID=MS.gene058828.t1.exon13;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12923752 | 12923856 | 12923752 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12924965 | 12925093 | 12924965 | ID=MS.gene058828.t1.exon14;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12924965 | 12925093 | 12924965 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12925436 | 12925539 | 12925436 | ID=MS.gene058828.t1.exon15;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12925436 | 12925539 | 12925436 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12925923 | 12926013 | 12925923 | ID=MS.gene058828.t1.exon16;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12925923 | 12926013 | 12925923 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| chr4.3 | exon | 12926110 | 12926190 | 12926110 | ID=MS.gene058828.t1.exon17;Parent=MS.gene058828.t1 |
| chr4.3 | CDS | 12926110 | 12926190 | 12926110 | ID=cds.MS.gene058828.t1;Parent=MS.gene058828.t1 |
| Gene Sequence |
| Protein sequence |
