Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55001.t1 | XP_022037687.1 | 50.7 | 75 | 37 | 0 | 39 | 113 | 74 | 148 | 7.50E-09 | 70.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55001.t1 | Q9LF68 | 78.4 | 37 | 8 | 0 | 75 | 111 | 140 | 176 | 3.8e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55001.t1 | A0A251UNX0 | 50.7 | 75 | 37 | 0 | 39 | 113 | 74 | 148 | 5.4e-09 | 70.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050156 | MS.gene55001 | 0.837385 | 5.36E-57 | -1.69E-46 |
| MS.gene051594 | MS.gene55001 | 0.808301 | 3.31E-50 | -1.69E-46 |
| MS.gene051957 | MS.gene55001 | 0.800391 | 1.47E-48 | -1.69E-46 |
| MS.gene052190 | MS.gene55001 | 0.803636 | 3.17E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene55001 | 0.808937 | 2.42E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene55001 | 0.802234 | 6.18E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene55001 | 0.841114 | 5.78E-58 | -1.69E-46 |
| MS.gene056173 | MS.gene55001 | 0.825918 | 3.61E-54 | -1.69E-46 |
| MS.gene056366 | MS.gene55001 | 0.802089 | 6.62E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene55001 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene058636 | MS.gene55001 | 0.809562 | 1.78E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene55001 | 0.80208 | 6.65E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55001.t1 | MTR_1g040145 | 70.213 | 47 | 14 | 0 | 67 | 113 | 114 | 160 | 4.28e-17 | 72.4 |
| MS.gene55001.t1 | MTR_7g027013 | 75.000 | 36 | 9 | 0 | 1 | 36 | 228 | 263 | 3.93e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55001.t1 | AT5G17560 | 78.378 | 37 | 8 | 0 | 75 | 111 | 140 | 176 | 3.18e-15 | 68.2 |
Find 26 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGCTTCAGCAGGAATTT+TGG | 0.218818 | 7.2:+24345819 | None:intergenic |
| CTGTTTGCGTTCTTGATGTT+CGG | 0.314904 | 7.2:-24346690 | MS.gene55001:CDS |
| TTTCTCAACCCGAAGTTGTC+TGG | 0.342503 | 7.2:-24347066 | MS.gene55001:CDS |
| AGGGAAAATCAGCCGTGAAT+AGG | 0.415276 | 7.2:-24345912 | MS.gene55001:CDS |
| GGATGGTGTACAAAGCCATA+TGG | 0.447903 | 7.2:-24345885 | MS.gene55001:CDS |
| ACTTTGAAGCTCCTCCCATA+TGG | 0.453900 | 7.2:+24345870 | None:intergenic |
| TTCTCAACCCGAAGTTGTCT+GGG | 0.474475 | 7.2:-24347065 | MS.gene55001:CDS |
| CTCCTCGTTTACCTTGGCAT+TGG | 0.487677 | 7.2:+24346660 | None:intergenic |
| GGTAAACGAGGAGCTTGAAA+TGG | 0.494180 | 7.2:-24346650 | MS.gene55001:CDS |
| TTCGGGTTGAGAAAACTGAT+TGG | 0.495426 | 7.2:+24347075 | None:intergenic |
| TTCAAGCTCCTCGTTTACCT+TGG | 0.502200 | 7.2:+24346654 | None:intergenic |
| AATCAGCCGTGAATAGGAAA+AGG | 0.505968 | 7.2:-24345906 | MS.gene55001:CDS |
| GGAGCTTGAAATGGTTATCA+TGG | 0.506912 | 7.2:-24346641 | MS.gene55001:CDS |
| CGTTCTTGATGTTCGGAATG+TGG | 0.526662 | 7.2:-24346683 | MS.gene55001:CDS |
| GATGGTGTACAAAGCCATAT+GGG | 0.535510 | 7.2:-24345884 | MS.gene55001:CDS |
| GAAAGCAGAAATTTACATTG+AGG | 0.550469 | 7.2:+24346712 | None:intergenic |
| AAGTTGTCTGGGAAAAGACT+TGG | 0.558569 | 7.2:-24347054 | MS.gene55001:CDS |
| AATGGTTATCATGGATACAA+AGG | 0.588295 | 7.2:-24346632 | MS.gene55001:intron |
| TCAGAGACGTGTTCTACGCA+TGG | 0.591087 | 7.2:-24347002 | MS.gene55001:CDS |
| TCGGAATGTGGCCAATGCCA+AGG | 0.603766 | 7.2:-24346671 | MS.gene55001:CDS |
| AGACGTGTTCTACGCATGGA+AGG | 0.618677 | 7.2:-24346998 | MS.gene55001:intron |
| AGTTGTCTGGGAAAAGACTT+GGG | 0.621946 | 7.2:-24347053 | MS.gene55001:CDS |
| GCTTCAAAGTGTAGTTCATG+TGG | 0.625499 | 7.2:-24345857 | MS.gene55001:CDS |
| AGCCGTGAATAGGAAAAGGA+TGG | 0.646525 | 7.2:-24345902 | MS.gene55001:CDS |
| GGCCAATGCCAAGGTAAACG+AGG | 0.684261 | 7.2:-24346662 | MS.gene55001:CDS |
| GGTGTACAAAGCCATATGGG+AGG | 0.708787 | 7.2:-24345881 | MS.gene55001:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATATAAAAAAAAGTATAAG+TGG | - | chr7.2:24346635-24346654 | MS.gene55001:CDS | 10.0% |
| !! | AGTATTAGGAAAAAAAATTT+AGG | - | chr7.2:24346669-24346688 | MS.gene55001:CDS | 15.0% |
| !! | TAAGCATTTATTCTAATTGA+CGG | - | chr7.2:24346037-24346056 | MS.gene55001:intron | 20.0% |
| !! | TGTTAAAATTCTGTGTAATT+TGG | - | chr7.2:24346317-24346336 | MS.gene55001:intron | 20.0% |
| !!! | ACGATTTTTTTGTCAAAATT+TGG | - | chr7.2:24346355-24346374 | MS.gene55001:intron | 20.0% |
| !!! | ATAAATGACTTTTGAAAATG+AGG | - | chr7.2:24346534-24346553 | MS.gene55001:intron | 20.0% |
| !!! | ATAGTTTTCTTTCATAAAGT+GGG | - | chr7.2:24346751-24346770 | MS.gene55001:intron | 20.0% |
| !!! | CGATTTTTTTGTCAAAATTT+GGG | - | chr7.2:24346356-24346375 | MS.gene55001:intron | 20.0% |
| !!! | TATTCATGTTCTATTTCTAA+GGG | + | chr7.2:24346498-24346517 | None:intergenic | 20.0% |
| !!! | TTGAATTAATAGTTGTGTTT+TGG | - | chr7.2:24346883-24346902 | MS.gene55001:intron | 20.0% |
| ! | AAGCATTTATTCTAATTGAC+GGG | - | chr7.2:24346038-24346057 | MS.gene55001:intron | 25.0% |
| !! | ATTGGTTGATATGTCTAATT+TGG | - | chr7.2:24346922-24346941 | MS.gene55001:intron | 25.0% |
| !! | GATAGTTTTCTTTCATAAAG+TGG | - | chr7.2:24346750-24346769 | MS.gene55001:intron | 25.0% |
| !!! | AACTTTATCATTAGCTTTGA+TGG | + | chr7.2:24346839-24346858 | None:intergenic | 25.0% |
| !!! | ATTCATGTTCTATTTCTAAG+GGG | + | chr7.2:24346497-24346516 | None:intergenic | 25.0% |
| !!! | CACATGTTTTAAAAACTCAA+TGG | + | chr7.2:24346140-24346159 | None:intergenic | 25.0% |
| !!! | CTATTCATGTTCTATTTCTA+AGG | + | chr7.2:24346499-24346518 | None:intergenic | 25.0% |
| !!! | TTGAGTTTTTAAAACATGTG+AGG | - | chr7.2:24346140-24346159 | MS.gene55001:intron | 25.0% |
| AACAACAATACACATCAAAC+TGG | + | chr7.2:24346301-24346320 | None:intergenic | 30.0% | |
| AAGTACATTGTGCTTAGATA+AGG | - | chr7.2:24346723-24346742 | MS.gene55001:CDS | 30.0% | |
| AATGGTTATCATGGATACAA+AGG | - | chr7.2:24346266-24346285 | MS.gene55001:intron | 30.0% | |
| ACAACAATACACATCAAACT+GGG | + | chr7.2:24346300-24346319 | None:intergenic | 30.0% | |
| CTAAATGACAACAAAGTGAT+AGG | - | chr7.2:24346415-24346434 | MS.gene55001:intron | 30.0% | |
| GAAAGCAGAAATTTACATTG+AGG | + | chr7.2:24346189-24346208 | None:intergenic | 30.0% | |
| TGATGCTAACACTACTATAT+AGG | + | chr7.2:24346079-24346098 | None:intergenic | 30.0% | |
| TGGTTTCAAATCACAGTATT+AGG | - | chr7.2:24346655-24346674 | MS.gene55001:CDS | 30.0% | |
| ! | CTTTATACTGACAGTTCTTT+TGG | + | chr7.2:24345961-24345980 | None:intergenic | 30.0% |
| !! | GTCATTTAGTTTCTCTGTTT+TGG | + | chr7.2:24346404-24346423 | None:intergenic | 30.0% |
| !! | TTCATGTTCTATTTCTAAGG+GGG | + | chr7.2:24346496-24346515 | None:intergenic | 30.0% |
| AATGACAACAAAGTGATAGG+AGG | - | chr7.2:24346418-24346437 | MS.gene55001:intron | 35.0% | |
| CAACAATACACATCAAACTG+GGG | + | chr7.2:24346299-24346318 | None:intergenic | 35.0% | |
| CTAATTCTATGAGCACAAGA+GGG | - | chr7.2:24346803-24346822 | MS.gene55001:intron | 35.0% | |
| GAAGTTATGATTGCCAAGTT+TGG | + | chr7.2:24346598-24346617 | None:intergenic | 35.0% | |
| GGACTCTACAATGTATAGAT+TGG | - | chr7.2:24346904-24346923 | MS.gene55001:intron | 35.0% | |
| TCTAATTCTATGAGCACAAG+AGG | - | chr7.2:24346802-24346821 | MS.gene55001:intron | 35.0% | |
| TCTGATCGCAACAATTGATA+TGG | - | chr7.2:24346101-24346120 | MS.gene55001:intron | 35.0% | |
| ! | AGAAGCTACTGACACAATTT+TGG | + | chr7.2:24346696-24346715 | None:intergenic | 35.0% |
| !!! | CAGGAATTTTGGTTATCATC+TGG | + | chr7.2:24347071-24347090 | None:intergenic | 35.0% |
| AAGTTGTCTGGGAAAAGACT+TGG | - | chr7.2:24345844-24345863 | MS.gene55001:CDS | 40.0% | |
| AATCAGCCGTGAATAGGAAA+AGG | - | chr7.2:24346992-24347011 | MS.gene55001:intron | 40.0% | |
| AGTTGTCTGGGAAAAGACTT+GGG | - | chr7.2:24345845-24345864 | MS.gene55001:CDS | 40.0% | |
| CAATTCTGCAATGCTACAGA+TGG | - | chr7.2:24345999-24346018 | MS.gene55001:intron | 40.0% | |
| CCAAGTTTGGTCAAACTAAG+TGG | + | chr7.2:24346585-24346604 | None:intergenic | 40.0% | |
| CTGTTTGCGTTCTTGATGTT+CGG | - | chr7.2:24346208-24346227 | MS.gene55001:intron | 40.0% | |
| GATGGTGTACAAAGCCATAT+GGG | - | chr7.2:24347014-24347033 | MS.gene55001:CDS | 40.0% | |
| GCTTCAAAGTGTAGTTCATG+TGG | - | chr7.2:24347041-24347060 | MS.gene55001:CDS | 40.0% | |
| GGAGCTTGAAATGGTTATCA+TGG | - | chr7.2:24346257-24346276 | MS.gene55001:intron | 40.0% | |
| GTTGTATCTGCAACCTTTGA+GGG | - | chr7.2:24346967-24346986 | MS.gene55001:intron | 40.0% | |
| TCTGTAGCATTGCAGAATTG+CGG | + | chr7.2:24345999-24346018 | None:intergenic | 40.0% | |
| TGTGTCAGTAGCTTCTTTCT+TGG | - | chr7.2:24346700-24346719 | MS.gene55001:CDS | 40.0% | |
| TGTTGTATCTGCAACCTTTG+AGG | - | chr7.2:24346966-24346985 | MS.gene55001:intron | 40.0% | |
| ! | AAGGGGGTTCATATTTCTGA+TGG | + | chr7.2:24346480-24346499 | None:intergenic | 40.0% |
| ! | AGTCTTTTCCCAGACAACTT+CGG | + | chr7.2:24345844-24345863 | None:intergenic | 40.0% |
| ! | CACCATCCTTTTCCTATTCA+CGG | + | chr7.2:24347001-24347020 | None:intergenic | 40.0% |
| ! | CCACTTAGTTTGACCAAACT+TGG | - | chr7.2:24346582-24346601 | MS.gene55001:intron | 40.0% |
| !! | TTCGGGTTGAGAAAACTGAT+TGG | + | chr7.2:24345826-24345845 | None:intergenic | 40.0% |
| AGCCGTGAATAGGAAAAGGA+TGG | - | chr7.2:24346996-24347015 | MS.gene55001:intron | 45.0% | |
| AGGGAAAATCAGCCGTGAAT+AGG | - | chr7.2:24346986-24347005 | MS.gene55001:intron | 45.0% | |
| ATTGCAGAATTGCGGCATCA+AGG | + | chr7.2:24345991-24346010 | None:intergenic | 45.0% | |
| CGTTCTTGATGTTCGGAATG+TGG | - | chr7.2:24346215-24346234 | MS.gene55001:intron | 45.0% | |
| GAGCACAAGAGGGTTCTTAT+TGG | - | chr7.2:24346813-24346832 | MS.gene55001:intron | 45.0% | |
| GGATGGTGTACAAAGCCATA+TGG | - | chr7.2:24347013-24347032 | MS.gene55001:CDS | 45.0% | |
| GGTAAACGAGGAGCTTGAAA+TGG | - | chr7.2:24346248-24346267 | MS.gene55001:intron | 45.0% | |
| GGTCAAACTAAGTGGTGTGT+TGG | + | chr7.2:24346577-24346596 | None:intergenic | 45.0% | |
| TTCAAGCTCCTCGTTTACCT+TGG | + | chr7.2:24346247-24346266 | None:intergenic | 45.0% | |
| TTCTCAACCCGAAGTTGTCT+GGG | - | chr7.2:24345833-24345852 | MS.gene55001:CDS | 45.0% | |
| TTTCTCAACCCGAAGTTGTC+TGG | - | chr7.2:24345832-24345851 | MS.gene55001:CDS | 45.0% | |
| ! | GTCTTTTCCCAGACAACTTC+GGG | + | chr7.2:24345843-24345862 | None:intergenic | 45.0% |
| !! | ACTTTGAAGCTCCTCCCATA+TGG | + | chr7.2:24347031-24347050 | None:intergenic | 45.0% |
| AGACGTGTTCTACGCATGGA+AGG | - | chr7.2:24345900-24345919 | MS.gene55001:CDS | 50.0% | |
| CTCCTCGTTTACCTTGGCAT+TGG | + | chr7.2:24346241-24346260 | None:intergenic | 50.0% | |
| GGTGTACAAAGCCATATGGG+AGG | - | chr7.2:24347017-24347036 | MS.gene55001:CDS | 50.0% | |
| TCAGAGACGTGTTCTACGCA+TGG | - | chr7.2:24345896-24345915 | MS.gene55001:CDS | 50.0% | |
| ! | GGCTGCTTCAGCAGGAATTT+TGG | + | chr7.2:24347082-24347101 | None:intergenic | 50.0% |
| !! | CACGGCTGATTTTCCCTCAA+AGG | + | chr7.2:24346983-24347002 | None:intergenic | 50.0% |
| GGCCAATGCCAAGGTAAACG+AGG | - | chr7.2:24346236-24346255 | MS.gene55001:intron | 55.0% | |
| TCGGAATGTGGCCAATGCCA+AGG | - | chr7.2:24346227-24346246 | MS.gene55001:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 24345816 | 24347104 | 24345816 | ID=MS.gene55001 |
| chr7.2 | mRNA | 24345816 | 24347104 | 24345816 | ID=MS.gene55001.t1;Parent=MS.gene55001 |
| chr7.2 | exon | 24346999 | 24347104 | 24346999 | ID=MS.gene55001.t1.exon1;Parent=MS.gene55001.t1 |
| chr7.2 | CDS | 24346999 | 24347104 | 24346999 | ID=cds.MS.gene55001.t1;Parent=MS.gene55001.t1 |
| chr7.2 | exon | 24346633 | 24346751 | 24346633 | ID=MS.gene55001.t1.exon2;Parent=MS.gene55001.t1 |
| chr7.2 | CDS | 24346633 | 24346751 | 24346633 | ID=cds.MS.gene55001.t1;Parent=MS.gene55001.t1 |
| chr7.2 | exon | 24345816 | 24345932 | 24345816 | ID=MS.gene55001.t1.exon3;Parent=MS.gene55001.t1 |
| chr7.2 | CDS | 24345816 | 24345932 | 24345816 | ID=cds.MS.gene55001.t1;Parent=MS.gene55001.t1 |
| Gene Sequence |
| Protein sequence |
