Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55870.t1 | XP_003630627.1 | 98.6 | 437 | 6 | 0 | 6 | 442 | 1 | 437 | 1.70E-245 | 858.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55870.t1 | Q8VYG2 | 65.0 | 432 | 140 | 2 | 11 | 442 | 3 | 423 | 5.2e-155 | 548.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55870.t1 | G7LJJ0 | 98.6 | 437 | 6 | 0 | 6 | 442 | 1 | 437 | 1.2e-245 | 858.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049178 | MS.gene55870 | 0.805053 | 1.61E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene55870 | 0.885388 | 8.49E-72 | -1.69E-46 |
| MS.gene050439 | MS.gene55870 | 0.801705 | 7.94E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene55870 | 0.829259 | 5.69E-55 | -1.69E-46 |
| MS.gene051885 | MS.gene55870 | 0.824607 | 7.37E-54 | -1.69E-46 |
| MS.gene052732 | MS.gene55870 | 0.813627 | 2.33E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene55870 | 0.853875 | 1.79E-61 | -1.69E-46 |
| MS.gene053581 | MS.gene55870 | 0.803893 | 2.80E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene55870 | 0.836238 | 1.05E-56 | -1.69E-46 |
| MS.gene054824 | MS.gene55870 | 0.800681 | 1.29E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene55870 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene55870 | 0.832457 | 9.35E-56 | -1.69E-46 |
| MS.gene055469 | MS.gene55870 | 0.828671 | 7.90E-55 | -1.69E-46 |
| MS.gene056043 | MS.gene55870 | -0.807557 | 4.77E-50 | -1.69E-46 |
| MS.gene056432 | MS.gene55870 | 0.812405 | 4.31E-51 | -1.69E-46 |
| MS.gene058639 | MS.gene55870 | 0.810743 | 9.91E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene55870 | 0.835749 | 1.40E-56 | -1.69E-46 |
| MS.gene059171 | MS.gene55870 | 0.839193 | 1.83E-57 | -1.69E-46 |
| MS.gene061006 | MS.gene55870 | 0.806045 | 9.95E-50 | -1.69E-46 |
| MS.gene061007 | MS.gene55870 | 0.807884 | 4.06E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55870.t1 | MTR_8g101540 | 98.627 | 437 | 6 | 0 | 6 | 442 | 1 | 437 | 0.0 | 897 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55870.t1 | AT3G10700 | 64.815 | 432 | 141 | 2 | 11 | 442 | 3 | 423 | 0.0 | 548 |
| MS.gene55870.t1 | AT3G10700 | 63.812 | 362 | 120 | 2 | 81 | 442 | 5 | 355 | 2.45e-154 | 441 |
| MS.gene55870.t1 | AT3G10700 | 64.907 | 322 | 102 | 2 | 11 | 332 | 3 | 313 | 6.31e-138 | 398 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGATTGAGAATGAATATT+TGG | 0.148149 | 4.3:-3678256 | MS.gene55870:CDS |
| CCCTATCGAATTTGTCCTTT+GGG | 0.178205 | 4.3:-3681129 | MS.gene55870:CDS |
| AAAATGTTGCTGGCTCTTTC+TGG | 0.184400 | 4.3:-3676774 | MS.gene55870:CDS |
| ACGGGAAGTTTAGAAGATTT+TGG | 0.201422 | 4.3:-3674809 | MS.gene55870:CDS |
| TTGAGAGCTCCTGGTGTATT+GGG | 0.238575 | 4.3:-3673448 | MS.gene55870:CDS |
| GAAATAATGCTCTGCTCTTT+TGG | 0.241028 | 4.3:+3674977 | None:intergenic |
| AGCCACAGGAACAATGTAAT+TGG | 0.256134 | 4.3:-3679995 | MS.gene55870:CDS |
| CTTCTGGGGTTTACTCCTTC+TGG | 0.272838 | 4.3:-3680849 | MS.gene55870:CDS |
| TTTAGCTATGACAATCAATA+AGG | 0.279528 | 4.3:-3680877 | MS.gene55870:CDS |
| GGAATTCTCATCGCTGCTTC+TGG | 0.291132 | 4.3:-3674788 | MS.gene55870:CDS |
| GGGGCACGTTTCAGTGGTGC+TGG | 0.313834 | 4.3:-3673427 | MS.gene55870:CDS |
| ATTGAGAGCTCCTGGTGTAT+TGG | 0.326421 | 4.3:-3673449 | MS.gene55870:CDS |
| AATCAATAAGGGAATACTTC+TGG | 0.338568 | 4.3:-3680865 | MS.gene55870:CDS |
| TTCAAGGAGAAGTGAAGTTC+AGG | 0.341185 | 4.3:-3680521 | MS.gene55870:intron |
| CTACCAACCCCGGATATAAT+AGG | 0.344598 | 4.3:-3676734 | MS.gene55870:CDS |
| GCCCTATCGAATTTGTCCTT+TGG | 0.353442 | 4.3:-3681130 | MS.gene55870:CDS |
| TTGTAGTTTGTAATTCGTTC+AGG | 0.355824 | 4.3:-3680549 | MS.gene55870:intron |
| CCGTATATGCACTACAGAAT+AGG | 0.363557 | 4.3:-3679953 | MS.gene55870:CDS |
| CGTATATGCACTACAGAATA+GGG | 0.363640 | 4.3:-3679952 | MS.gene55870:CDS |
| CAATGTTTACAGGGTATCAT+TGG | 0.367185 | 4.3:-3679092 | MS.gene55870:intron |
| GCCACAGGAACAATGTAATT+GGG | 0.370262 | 4.3:-3679994 | MS.gene55870:CDS |
| TTGATTGAGAATGAATATTT+GGG | 0.370505 | 4.3:-3678255 | MS.gene55870:CDS |
| TTCAGTGGTGCTGGGTTTAG+AGG | 0.370802 | 4.3:-3673418 | MS.gene55870:CDS |
| TCTGATGGTCTGGACAGTTC+AGG | 0.372167 | 4.3:-3679056 | MS.gene55870:CDS |
| CCTATTCTGTAGTGCATATA+CGG | 0.381651 | 4.3:+3679953 | None:intergenic |
| GGAGTCGAGGCTTGGGAAAC+GGG | 0.384982 | 4.3:-3674827 | MS.gene55870:CDS |
| AATTAATTCTGCAGATCATC+TGG | 0.392142 | 4.3:-3676266 | MS.gene55870:intron |
| TGCAACTCGCCTATTATATC+CGG | 0.393949 | 4.3:+3676725 | None:intergenic |
| ATCAATAAGGGAATACTTCT+GGG | 0.405069 | 4.3:-3680864 | MS.gene55870:CDS |
| AGTAGCATACCTCTTAGCTT+TGG | 0.420578 | 4.3:-3678933 | MS.gene55870:CDS |
| GAAAGCAGCTAAAATGTTGC+TGG | 0.429654 | 4.3:-3676784 | MS.gene55870:CDS |
| CTGAAAATATTGAATATGAT+AGG | 0.435242 | 4.3:-3678880 | MS.gene55870:intron |
| ATACACCAGGAGCTCTCAAT+AGG | 0.436295 | 4.3:+3673452 | None:intergenic |
| TTGTGAGCCTCATAAACTTC+CGG | 0.440960 | 4.3:+3676080 | None:intergenic |
| GGGCACGTTTCAGTGGTGCT+GGG | 0.443328 | 4.3:-3673426 | MS.gene55870:CDS |
| TTAGCTATGACAATCAATAA+GGG | 0.443756 | 4.3:-3680876 | MS.gene55870:CDS |
| TCTGACTAGCTAACTCGGGC+TGG | 0.445555 | 4.3:+3673320 | None:intergenic |
| TAATCCAGGAGTCGAGGCTT+GGG | 0.458763 | 4.3:-3674834 | MS.gene55870:intron |
| AGGAGTCGAGGCTTGGGAAA+CGG | 0.463752 | 4.3:-3674828 | MS.gene55870:CDS |
| GAGAGAGAAAGTTTCTCAAA+TGG | 0.464031 | 4.3:-3681190 | MS.gene55870:CDS |
| TGTACTCTTGCACCGTTGGC+CGG | 0.464779 | 4.3:+3676823 | None:intergenic |
| CATACGTGGTTCTGATGGTC+TGG | 0.468452 | 4.3:-3679066 | MS.gene55870:CDS |
| CTGAAGCTGCATCATTTGTC+AGG | 0.475935 | 4.3:-3673360 | MS.gene55870:CDS |
| TTTGTCTTAGTAGTTTGCAC+TGG | 0.480855 | 4.3:+3680047 | None:intergenic |
| GCAACTCGCCTATTATATCC+GGG | 0.484604 | 4.3:+3676726 | None:intergenic |
| AGTTGCACCGGAAGTTTATG+AGG | 0.486774 | 4.3:-3676087 | MS.gene55870:CDS |
| TTGCATGTTCCAAAGCTAAG+AGG | 0.495586 | 4.3:+3678924 | None:intergenic |
| ATTCGTTCAGGACAGTTTCA+AGG | 0.498096 | 4.3:-3680537 | MS.gene55870:CDS |
| GCTTATCTGACTAGCTAACT+CGG | 0.498921 | 4.3:+3673315 | None:intergenic |
| ATTACATTGTTCCTGTGGCT+CGG | 0.509440 | 4.3:+3679998 | None:intergenic |
| TTAATCCAGGAGTCGAGGCT+TGG | 0.509901 | 4.3:-3674835 | MS.gene55870:intron |
| GAGATCCTATTGAGAGCTCC+TGG | 0.513071 | 4.3:-3673457 | MS.gene55870:CDS |
| TGAGAGCTCCTGGTGTATTG+GGG | 0.515368 | 4.3:-3673447 | MS.gene55870:CDS |
| GCGGAGCACATCCTGTCAAA+TGG | 0.519126 | 4.3:-3676233 | MS.gene55870:intron |
| AGAGAATTCTTCCGAGCCAC+AGG | 0.528821 | 4.3:-3680009 | MS.gene55870:CDS |
| CCTATCGAATTTGTCCTTTG+GGG | 0.530180 | 4.3:-3681128 | MS.gene55870:CDS |
| GTGCAAATTAGAACCCGATC+TGG | 0.532694 | 4.3:-3675000 | MS.gene55870:CDS |
| CTATCGAATTTGTCCTTTGG+GGG | 0.532892 | 4.3:-3681127 | MS.gene55870:CDS |
| TCAAATACTGACCATTTGAC+AGG | 0.535076 | 4.3:+3676222 | None:intergenic |
| TTGAAAGTAGTATAGCTGAT+TGG | 0.535673 | 4.3:+3678211 | None:intergenic |
| TCCATTCAAAATTATGAATG+TGG | 0.538112 | 4.3:-3674758 | MS.gene55870:intron |
| TTCTCATCGCTGCTTCTGGT+CGG | 0.543594 | 4.3:-3674784 | MS.gene55870:CDS |
| GAGAATATGCGTGTTATGAA+AGG | 0.544922 | 4.3:-3674951 | MS.gene55870:intron |
| CTGACTAGCTAACTCGGGCT+GGG | 0.547001 | 4.3:+3673321 | None:intergenic |
| AGGATGCTGTATTGCCCTTG+TGG | 0.556238 | 4.3:-3673398 | MS.gene55870:CDS |
| CCCCAATTACATTGTTCCTG+TGG | 0.559811 | 4.3:+3679993 | None:intergenic |
| GCGAGTTGCAGAGTGTAAAG+AGG | 0.567225 | 4.3:-3676712 | MS.gene55870:CDS |
| CCCAAAGGACAAATTCGATA+GGG | 0.568698 | 4.3:+3681129 | None:intergenic |
| AGAAGATGGCAGGGTCGTGT+TGG | 0.579641 | 4.3:-3681667 | MS.gene55870:CDS |
| GAAAGTTTCTCAAATGGCAA+AGG | 0.583814 | 4.3:-3681184 | MS.gene55870:CDS |
| CCACAGGAACAATGTAATTG+GGG | 0.584095 | 4.3:-3679993 | MS.gene55870:CDS |
| GGATACATACGTGGTTCTGA+TGG | 0.587638 | 4.3:-3679071 | MS.gene55870:CDS |
| TTCACCTCATCGCTTCCAGA+AGG | 0.589446 | 4.3:+3680834 | None:intergenic |
| ATCTCATACAGTTGAATCAG+TGG | 0.591158 | 4.3:+3673475 | None:intergenic |
| CTTATCTGACTAGCTAACTC+GGG | 0.593706 | 4.3:+3673316 | None:intergenic |
| TCGCCTATTATATCCGGGGT+TGG | 0.597810 | 4.3:+3676731 | None:intergenic |
| TAACGTACGTACCTCTGTAT+CGG | 0.598872 | 4.3:+3681640 | None:intergenic |
| TAGGGGACACAATATTTCAA+AGG | 0.601696 | 4.3:-3679934 | MS.gene55870:intron |
| TTTCTTCCAAGCAGTTGCAC+CGG | 0.608122 | 4.3:-3676099 | MS.gene55870:intron |
| TAGCAAGGTGCTCCTCCACA+AGG | 0.611891 | 4.3:+3673383 | None:intergenic |
| AATGTTTAATCCAGGAGTCG+AGG | 0.613521 | 4.3:-3674840 | MS.gene55870:intron |
| AAGAATATAAGCTTATTCAC+CGG | 0.614977 | 4.3:-3676842 | MS.gene55870:CDS |
| CCCCAAAGGACAAATTCGAT+AGG | 0.615725 | 4.3:+3681128 | None:intergenic |
| GAGAGCTCCTGGTGTATTGG+GGG | 0.618129 | 4.3:-3673446 | MS.gene55870:CDS |
| ATGATGCAGCTTCAGTAGCA+AGG | 0.619433 | 4.3:+3673368 | None:intergenic |
| TTTGATGTGCATGAATTGCA+AGG | 0.621600 | 4.3:-3678178 | MS.gene55870:intron |
| ATGCTGTATTGCCCTTGTGG+AGG | 0.622412 | 4.3:-3673395 | MS.gene55870:CDS |
| TACTCCTTCTGGAAGCGATG+AGG | 0.627318 | 4.3:-3680838 | MS.gene55870:intron |
| GGAAGTTTATGAGGCTCACA+AGG | 0.632515 | 4.3:-3676078 | MS.gene55870:intron |
| GCTATACTACTTTCAAGACA+TGG | 0.632611 | 4.3:-3678204 | MS.gene55870:CDS |
| GTACGTACCTCTGTATCGGA+AGG | 0.632909 | 4.3:+3681644 | None:intergenic |
| TCAATAAGGGAATACTTCTG+GGG | 0.643607 | 4.3:-3680863 | MS.gene55870:CDS |
| CAACTCGCCTATTATATCCG+GGG | 0.650891 | 4.3:+3676727 | None:intergenic |
| TAAGCTTATTCACCGGCCAA+CGG | 0.652544 | 4.3:-3676835 | MS.gene55870:CDS |
| ATCATCTGGAGATTATGAAG+CGG | 0.659188 | 4.3:-3676252 | MS.gene55870:CDS |
| CAAGCTTTGACTACCAACCC+CGG | 0.668548 | 4.3:-3676744 | MS.gene55870:CDS |
| GTGTTGGCCTTCCGATACAG+AGG | 0.670297 | 4.3:-3681651 | MS.gene55870:intron |
| CACAGGAACAATGTAATTGG+GGG | 0.671775 | 4.3:-3679992 | MS.gene55870:CDS |
| AGCAAGGTGCTCCTCCACAA+GGG | 0.672873 | 4.3:+3673384 | None:intergenic |
| TGTCAGGAGAGAATATCTCA+AGG | 0.688973 | 4.3:-3673344 | MS.gene55870:CDS |
| GTATATGCACTACAGAATAG+GGG | 0.695498 | 4.3:-3679951 | MS.gene55870:CDS |
| GGTATCATTGGATACATACG+TGG | 0.735502 | 4.3:-3679080 | MS.gene55870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 3673261 | 3681934 | 3673261 | ID=MS.gene55870 |
| chr4.3 | mRNA | 3673261 | 3681934 | 3673261 | ID=MS.gene55870.t1;Parent=MS.gene55870 |
| chr4.3 | exon | 3681932 | 3681934 | 3681932 | ID=MS.gene55870.t1.exon1;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3681932 | 3681934 | 3681932 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3681652 | 3681696 | 3681652 | ID=MS.gene55870.t1.exon2;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3681652 | 3681696 | 3681652 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3681110 | 3681223 | 3681110 | ID=MS.gene55870.t1.exon3;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3681110 | 3681223 | 3681110 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3680839 | 3680910 | 3680839 | ID=MS.gene55870.t1.exon4;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3680839 | 3680910 | 3680839 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3680522 | 3680565 | 3680522 | ID=MS.gene55870.t1.exon5;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3680522 | 3680565 | 3680522 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3679935 | 3680088 | 3679935 | ID=MS.gene55870.t1.exon6;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3679935 | 3680088 | 3679935 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3679037 | 3679102 | 3679037 | ID=MS.gene55870.t1.exon7;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3679037 | 3679102 | 3679037 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3678881 | 3678960 | 3678881 | ID=MS.gene55870.t1.exon8;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3678881 | 3678960 | 3678881 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3678179 | 3678278 | 3678179 | ID=MS.gene55870.t1.exon9;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3678179 | 3678278 | 3678179 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3676693 | 3676868 | 3676693 | ID=MS.gene55870.t1.exon10;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3676693 | 3676868 | 3676693 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3676234 | 3676274 | 3676234 | ID=MS.gene55870.t1.exon11;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3676234 | 3676274 | 3676234 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3676079 | 3676107 | 3676079 | ID=MS.gene55870.t1.exon12;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3676079 | 3676107 | 3676079 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3674952 | 3675021 | 3674952 | ID=MS.gene55870.t1.exon13;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3674952 | 3675021 | 3674952 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3674759 | 3674848 | 3674759 | ID=MS.gene55870.t1.exon14;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3674759 | 3674848 | 3674759 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| chr4.3 | exon | 3673261 | 3673505 | 3673261 | ID=MS.gene55870.t1.exon15;Parent=MS.gene55870.t1 |
| chr4.3 | CDS | 3673261 | 3673505 | 3673261 | ID=cds.MS.gene55870.t1;Parent=MS.gene55870.t1 |
| Gene Sequence |
| Protein sequence |
