AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56249


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56249.t1 MTR_3g009020 95.378 238 11 0 1 238 254 491 1.05e-169 477
MS.gene56249.t1 MTR_3g009030 83.636 220 36 0 1 220 278 497 1.33e-136 393
MS.gene56249.t1 MTR_3g009010 80.909 220 42 0 1 220 343 562 1.74e-129 377
MS.gene56249.t1 MTR_3g008960 78.182 220 48 0 1 220 270 489 6.97e-126 365
MS.gene56249.t1 MTR_8g091310 66.063 221 74 1 1 220 286 506 3.99e-104 310
MS.gene56249.t1 MTR_5g062190 53.982 226 98 4 1 220 282 507 4.64e-79 246
MS.gene56249.t1 MTR_5g062290 51.327 226 104 4 1 220 280 505 1.44e-72 229
MS.gene56249.t1 MTR_3g086750 45.133 226 118 4 1 220 280 505 8.33e-59 194
MS.gene56249.t1 MTR_4g035760 46.528 144 71 2 83 220 36 179 1.04e-41 140
MS.gene56249.t1 MTR_5g061980 47.826 92 46 2 76 165 219 310 2.31e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATTACTACAATATGTTT+TGG 0.227561 3.3:-4546834 MS.gene56249:CDS
CAACATTTGGATGGGCTTAT+TGG 0.274922 3.3:-4546288 MS.gene56249:CDS
ATTGAGCTTGGGCAAAGTTT+TGG 0.283062 3.3:+4546329 None:intergenic
GCAATTGAAGGTTCTCAAAA+TGG 0.284192 3.3:-4547500 MS.gene56249:CDS
AAACTTTGCCCAAGCTCAAT+TGG 0.353354 3.3:-4546326 MS.gene56249:CDS
GCTCTTCTCTCATTTGTTAG+TGG 0.361357 3.3:-4546880 MS.gene56249:CDS
AATTAATCACAGCGACTGTC+TGG 0.361757 3.3:+4547420 None:intergenic
TTGCTTAGACGCACTTTGTA+TGG 0.372643 3.3:+4546357 None:intergenic
ACGATTAAACTGGCATTTGT+AGG 0.378607 3.3:+4546264 None:intergenic
TATGGTAATAAACCAAGTTT+AGG 0.379061 3.3:-4547045 MS.gene56249:CDS
CAAAATGGCAATGAACATAG+TGG 0.392126 3.3:-4547485 MS.gene56249:CDS
GTGTATTCTCGGGCAACATT+TGG 0.393337 3.3:-4546301 MS.gene56249:CDS
CTCAATTGGATGTGTATTCT+CGG 0.396743 3.3:-4546312 MS.gene56249:CDS
CAAGAGATGGATATATAGAA+TGG 0.417101 3.3:-4547460 MS.gene56249:CDS
TAGTCTCAAGAAGTATTATA+AGG 0.418460 3.3:-4546980 MS.gene56249:CDS
AAGAGATGGATATATAGAAT+GGG 0.434894 3.3:-4547459 MS.gene56249:CDS
AGCTCTATTCCTCCTAAACT+TGG 0.449256 3.3:+4547033 None:intergenic
TTCTCGGGCAACATTTGGAT+GGG 0.450046 3.3:-4546296 MS.gene56249:CDS
TGAGACTCCAACGATTAAAC+TGG 0.451572 3.3:+4546254 None:intergenic
GAAAGAGCCTCAGATCTTGC+TGG 0.453188 3.3:-4546760 MS.gene56249:CDS
GACATAATCACATAAGCCTC+TGG 0.478424 3.3:+4546928 None:intergenic
TGTGATTATGTCAAATCCAT+TGG 0.497659 3.3:-4546917 MS.gene56249:CDS
GTGGACTTGCATGCAATTGA+AGG 0.498572 3.3:-4547512 MS.gene56249:CDS
ATTCTCGGGCAACATTTGGA+TGG 0.503691 3.3:-4546297 MS.gene56249:CDS
AATACACATCCAATTGAGCT+TGG 0.517212 3.3:+4546317 None:intergenic
ATTAATCACAGCGACTGTCT+GGG 0.528222 3.3:+4547421 None:intergenic
TAGGGGAATGGACGGGTGAG+TGG 0.529808 3.3:-4546381 MS.gene56249:CDS
ATACACATCCAATTGAGCTT+GGG 0.532963 3.3:+4546318 None:intergenic
CTCAAGTGGATGATCGAGAA+TGG 0.534717 3.3:-4546235 MS.gene56249:CDS
GGTAATAAACCAAGTTTAGG+AGG 0.535992 3.3:-4547042 MS.gene56249:CDS
GCTTTAGAATCTCCATCCAA+TGG 0.542610 3.3:+4546901 None:intergenic
GAACATAGTGGGACAAGAGA+TGG 0.543354 3.3:-4547473 MS.gene56249:CDS
ACCAACAGATAGCTTTCGAC+AGG 0.544967 3.3:-4546185 MS.gene56249:CDS
TCAATTGGATGTGTATTCTC+GGG 0.550495 3.3:-4546311 MS.gene56249:CDS
ACAAATGCCAGTTTAATCGT+TGG 0.551584 3.3:-4546261 MS.gene56249:CDS
CCATGGGATGAAAGTGATTG+TGG 0.555733 3.3:-4547531 None:intergenic
TCCTGTCGAAAGCTATCTGT+TGG 0.560585 3.3:+4546184 None:intergenic
TTAATCGTTGGAGTCTCAAG+TGG 0.586204 3.3:-4546249 MS.gene56249:CDS
CTATCAAGATTGACACCTTG+TGG 0.598476 3.3:+4547000 None:intergenic
ATTATGTCAAATCCATTGGA+TGG 0.603936 3.3:-4546913 MS.gene56249:CDS
TTAATCACAGCGACTGTCTG+GGG 0.606862 3.3:+4547422 None:intergenic
TGATTAATTTCTTAGCACAA+AGG 0.607927 3.3:-4547406 MS.gene56249:intron
AAAATGGCAATGAACATAGT+GGG 0.608116 3.3:-4547484 MS.gene56249:CDS
TATTGTAGTAATGCACATCA+AGG 0.609097 3.3:+4546842 None:intergenic
ATAGCTTTCGACAGGATGTG+AGG 0.613651 3.3:-4546177 MS.gene56249:CDS
TAGCTTTCGACAGGATGTGA+GGG 0.624203 3.3:-4546176 MS.gene56249:CDS
AGAGATGGATATATAGAATG+GGG 0.625469 3.3:-4547458 MS.gene56249:CDS
AGATACACCAGCAAGATCTG+AGG 0.672584 3.3:+4546753 None:intergenic
TGTGAGAAAATACAAGCCAG+AGG 0.723983 3.3:-4546944 MS.gene56249:CDS
GAGCTGATGAATGAACCACA+AGG 0.784741 3.3:-4547015 MS.gene56249:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCTATTTCTAATCAATTT+GGG - chr3.3:4546519-4546538 MS.gene56249:intron 15.0%
!!! ATATGTTTTTTCTTTTTGTA+GGG - chr3.3:4547290-4547309 MS.gene56249:intron 15.0%
!!! TAATCTATTTCTAATCAATT+TGG - chr3.3:4546518-4546537 MS.gene56249:intron 15.0%
!!! TATATGTTTTTTCTTTTTGT+AGG - chr3.3:4547289-4547308 MS.gene56249:intron 15.0%
!! AACTCATCCATATTATATAT+CGG - chr3.3:4547259-4547278 MS.gene56249:intron 20.0%
!! ACATATATGAAATATGACAA+AGG - chr3.3:4547054-4547073 MS.gene56249:intron 20.0%
!! ATCTAATTTCTTCTCTATAT+TGG - chr3.3:4546324-4546343 MS.gene56249:CDS 20.0%
!! ATGAGTTAGTATATATTTCA+TGG + chr3.3:4547246-4547265 None:intergenic 20.0%
!! CATATAGATACCAATAAAAT+TGG + chr3.3:4547005-4547024 None:intergenic 20.0%
!! CATGTATAATTCAATTACTT+GGG + chr3.3:4546601-4546620 None:intergenic 20.0%
!! TATCAAATATCATATATTCC+TGG + chr3.3:4546567-4546586 None:intergenic 20.0%
!! TATTGGAATTTAAGTATAAG+TGG - chr3.3:4546341-4546360 MS.gene56249:CDS 20.0%
!! TGAGTTAGTATATATTTCAT+GGG + chr3.3:4547245-4547264 None:intergenic 20.0%
!!! AAATTGATAATATGTGAAAG+AGG + chr3.3:4546491-4546510 None:intergenic 20.0%
!!! TATGTTTTTTCTTTTTGTAG+GGG - chr3.3:4547291-4547310 MS.gene56249:intron 20.0%
! AAGAGATGGATATATAGAAT+GGG - chr3.3:4546230-4546249 MS.gene56249:CDS 25.0%
! ACTCATCCATATTATATATC+GGG - chr3.3:4547260-4547279 MS.gene56249:intron 25.0%
! AGATACCAATAAAATTGGTT+AGG + chr3.3:4547000-4547019 None:intergenic 25.0%
! CAATAAAATTGGTTAGGATT+TGG + chr3.3:4546994-4547013 None:intergenic 25.0%
! GCATGTATAATTCAATTACT+TGG + chr3.3:4546602-4546621 None:intergenic 25.0%
! TATGGTAATAAACCAAGTTT+AGG - chr3.3:4546644-4546663 MS.gene56249:intron 25.0%
! TGATTAATTTCTTAGCACAA+AGG - chr3.3:4546283-4546302 MS.gene56249:CDS 25.0%
!! CAAATCCTAACCAATTTTAT+TGG - chr3.3:4546992-4547011 MS.gene56249:CDS 25.0%
!! GCATTACTACAATATGTTTT+GGG - chr3.3:4546856-4546875 MS.gene56249:CDS 25.0%
!! TGCATTACTACAATATGTTT+TGG - chr3.3:4546855-4546874 MS.gene56249:CDS 25.0%
!!! TAGTCTCAAGAAGTATTATA+AGG - chr3.3:4546709-4546728 MS.gene56249:intron 25.0%
AAAATGGCAATGAACATAGT+GGG - chr3.3:4546205-4546224 MS.gene56249:CDS 30.0%
AGAGATGGATATATAGAATG+GGG - chr3.3:4546231-4546250 MS.gene56249:CDS 30.0%
ATTATGTCAAATCCATTGGA+TGG - chr3.3:4546776-4546795 MS.gene56249:CDS 30.0%
CAAGAGATGGATATATAGAA+TGG - chr3.3:4546229-4546248 MS.gene56249:CDS 30.0%
CTCATCCATATTATATATCG+GGG - chr3.3:4547261-4547280 MS.gene56249:intron 30.0%
GTATCTATATGTGTGTGATA+GGG - chr3.3:4547014-4547033 MS.gene56249:CDS 30.0%
GTTATCCCCGATATATAATA+TGG + chr3.3:4547269-4547288 None:intergenic 30.0%
TATTGTAGTAATGCACATCA+AGG + chr3.3:4546850-4546869 None:intergenic 30.0%
TGGAATTTAAGTATAAGTGG+TGG - chr3.3:4546344-4546363 MS.gene56249:CDS 30.0%
TGTGATTATGTCAAATCCAT+TGG - chr3.3:4546772-4546791 MS.gene56249:CDS 30.0%
TTGCTTAACATGTGTCATAA+GGG - chr3.3:4547204-4547223 MS.gene56249:intron 30.0%
! TTTTTCTTTTTGTAGGGGAA+TGG - chr3.3:4547296-4547315 MS.gene56249:intron 30.0%
!! ACAAATGCTCTTACTTTTGT+TGG - chr3.3:4546962-4546981 MS.gene56249:CDS 30.0%
!! ATTGAATTTGTTGACAGGTA+TGG - chr3.3:4546626-4546645 MS.gene56249:intron 30.0%
!! CATGAATTGAATTTGTTGAC+AGG - chr3.3:4546621-4546640 MS.gene56249:intron 30.0%
!!! ATGTTTTGGGACAAATTCAA+TGG - chr3.3:4546869-4546888 MS.gene56249:CDS 30.0%
AATACACATCCAATTGAGCT+TGG + chr3.3:4547375-4547394 None:intergenic 35.0%
ACAAATGCCAGTTTAATCGT+TGG - chr3.3:4547428-4547447 MS.gene56249:CDS 35.0%
ACGATTAAACTGGCATTTGT+AGG + chr3.3:4547428-4547447 None:intergenic 35.0%
ATACACATCCAATTGAGCTT+GGG + chr3.3:4547374-4547393 None:intergenic 35.0%
CAAAAAGAGCAACTTTGCAT+TGG + chr3.3:4547182-4547201 None:intergenic 35.0%
CAAAATGGCAATGAACATAG+TGG - chr3.3:4546204-4546223 MS.gene56249:CDS 35.0%
CTCAATTGGATGTGTATTCT+CGG - chr3.3:4547377-4547396 MS.gene56249:intron 35.0%
GCAATTGAAGGTTCTCAAAA+TGG - chr3.3:4546189-4546208 MS.gene56249:CDS 35.0%
GGTAATAAACCAAGTTTAGG+AGG - chr3.3:4546647-4546666 MS.gene56249:intron 35.0%
GGTATCTATATGTGTGTGAT+AGG - chr3.3:4547013-4547032 MS.gene56249:CDS 35.0%
GTTGCTTAACATGTGTCATA+AGG - chr3.3:4547203-4547222 MS.gene56249:intron 35.0%
TCAATTGGATGTGTATTCTC+GGG - chr3.3:4547378-4547397 MS.gene56249:intron 35.0%
AAACTTTGCCCAAGCTCAAT+TGG - chr3.3:4547363-4547382 MS.gene56249:intron 40.0%
AATTAATCACAGCGACTGTC+TGG + chr3.3:4546272-4546291 None:intergenic 40.0%
AGCTCTATTCCTCCTAAACT+TGG + chr3.3:4546659-4546678 None:intergenic 40.0%
ATTAATCACAGCGACTGTCT+GGG + chr3.3:4546271-4546290 None:intergenic 40.0%
CTATCAAGATTGACACCTTG+TGG + chr3.3:4546692-4546711 None:intergenic 40.0%
GACATAATCACATAAGCCTC+TGG + chr3.3:4546764-4546783 None:intergenic 40.0%
TCCACTCACACTTAAATTCC+AGG - chr3.3:4546546-4546565 MS.gene56249:intron 40.0%
TCCTGGAATTTAAGTGTGAG+TGG + chr3.3:4546550-4546569 None:intergenic 40.0%
TGAGACTCCAACGATTAAAC+TGG + chr3.3:4547438-4547457 None:intergenic 40.0%
TGTGAGAAAATACAAGCCAG+AGG - chr3.3:4546745-4546764 MS.gene56249:CDS 40.0%
TTAATCGTTGGAGTCTCAAG+TGG - chr3.3:4547440-4547459 MS.gene56249:CDS 40.0%
TTGCTTAGACGCACTTTGTA+TGG + chr3.3:4547335-4547354 None:intergenic 40.0%
! AGTGGTGGTTAGTCACATAT+TGG - chr3.3:4546359-4546378 MS.gene56249:CDS 40.0%
! ATTGAGCTTGGGCAAAGTTT+TGG + chr3.3:4547363-4547382 None:intergenic 40.0%
! CAACATTTGGATGGGCTTAT+TGG - chr3.3:4547401-4547420 MS.gene56249:intron 40.0%
! GCTCTTCTCTCATTTGTTAG+TGG - chr3.3:4546809-4546828 MS.gene56249:CDS 40.0%
! TCTTTTTGTAGGGGAATGGA+CGG - chr3.3:4547300-4547319 MS.gene56249:intron 40.0%
!! GCTTTAGAATCTCCATCCAA+TGG + chr3.3:4546791-4546810 None:intergenic 40.0%
ACCAACAGATAGCTTTCGAC+AGG - chr3.3:4547504-4547523 MS.gene56249:CDS 45.0%
AGATACACCAGCAAGATCTG+AGG + chr3.3:4546939-4546958 None:intergenic 45.0%
ATAGCTTTCGACAGGATGTG+AGG - chr3.3:4547512-4547531 MS.gene56249:CDS 45.0%
ATTCTCGGGCAACATTTGGA+TGG - chr3.3:4547392-4547411 MS.gene56249:intron 45.0%
CTCAAGTGGATGATCGAGAA+TGG - chr3.3:4547454-4547473 MS.gene56249:CDS 45.0%
GAGCTGATGAATGAACCACA+AGG - chr3.3:4546674-4546693 MS.gene56249:intron 45.0%
GTGGACTTGCATGCAATTGA+AGG - chr3.3:4546177-4546196 MS.gene56249:CDS 45.0%
GTGTATTCTCGGGCAACATT+TGG - chr3.3:4547388-4547407 MS.gene56249:intron 45.0%
TAGCTTTCGACAGGATGTGA+GGG - chr3.3:4547513-4547532 MS.gene56249:CDS 45.0%
TCCTGTCGAAAGCTATCTGT+TGG + chr3.3:4547508-4547527 None:intergenic 45.0%
TCCTTAACATGTGCCCTTGA+AGG + chr3.3:4547090-4547109 None:intergenic 45.0%
TTAATCACAGCGACTGTCTG+GGG + chr3.3:4546270-4546289 None:intergenic 45.0%
TTCTCGGGCAACATTTGGAT+GGG - chr3.3:4547393-4547412 MS.gene56249:intron 45.0%
! AAGGCTAACTTGTGCCTTCA+AGG - chr3.3:4547073-4547092 MS.gene56249:intron 45.0%
! AGGCTAACTTGTGCCTTCAA+GGG - chr3.3:4547074-4547093 MS.gene56249:intron 45.0%
! CTTCTGACGGACATACTTGT+AGG + chr3.3:4546429-4546448 None:intergenic 45.0%
! CTTTTTGTAGGGGAATGGAC+GGG - chr3.3:4547301-4547320 MS.gene56249:intron 45.0%
! GAACATAGTGGGACAAGAGA+TGG - chr3.3:4546216-4546235 MS.gene56249:CDS 45.0%
CTTACTCGCTACGCTTCTGA+CGG + chr3.3:4546442-4546461 None:intergenic 50.0%
GAAAGAGCCTCAGATCTTGC+TGG - chr3.3:4546929-4546948 MS.gene56249:CDS 50.0%
! GCCTTCAAGGGCACATGTTA+AGG - chr3.3:4547086-4547105 MS.gene56249:intron 50.0%
TAGGGGAATGGACGGGTGAG+TGG - chr3.3:4547308-4547327 MS.gene56249:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 4546165 4547546 4546165 ID=MS.gene56249
chr3.3 mRNA 4546165 4547546 4546165 ID=MS.gene56249.t1;Parent=MS.gene56249
chr3.3 exon 4547407 4547546 4547407 ID=MS.gene56249.t1.exon1;Parent=MS.gene56249.t1
chr3.3 CDS 4547407 4547546 4547407 ID=cds.MS.gene56249.t1;Parent=MS.gene56249.t1
chr3.3 exon 4546728 4547068 4546728 ID=MS.gene56249.t1.exon2;Parent=MS.gene56249.t1
chr3.3 CDS 4546728 4547068 4546728 ID=cds.MS.gene56249.t1;Parent=MS.gene56249.t1
chr3.3 exon 4546165 4546400 4546165 ID=MS.gene56249.t1.exon3;Parent=MS.gene56249.t1
chr3.3 CDS 4546165 4546400 4546165 ID=cds.MS.gene56249.t1;Parent=MS.gene56249.t1
Gene Sequence

>MS.gene56249

ATGAAAGTGATTGTGGACTTGCATGCAATTGAAGGTTCTCAAAATGGCAATGAACATAGTGGGACAAGAGATGGATATATAGAATGGGGTGATTCTTACATACCCCAGACAGTCGCTGTGATTAATTTCTTAGCACAAAGGTTTTGTATCTATATAATCATCTAATTTCTTCTCTATATTGGAATTTAAGTATAAGTGGTGGTTAGTCACATATTGGATACAAATAGATGTAGTAGAATATGCAACTATGAGTAGTTATTACCTACAAGTATGTCCGTCAGAAGCGTAGCGAGTAAGACTTAAGACATTACATATATGAGTCACCTCTTTCACATATTATCAATTTAATCTATTAATCTATTTCTAATCAATTTGGGACTATCCACTCACACTTAAATTCCAGGAATATATGATATTTGATATGATGTTATGACCCAAGTAATTGAATTATACATGCATGAATTGAATTTGTTGACAGGTATGGTAATAAACCAAGTTTAGGAGGAATAGAGCTGATGAATGAACCACAAGGTGTCAATCTTGATAGTCTCAAGAAGTATTATAAGGCAGCTTATGATGCTGTGAGAAAATACAAGCCAGAGGCTTATGTGATTATGTCAAATCCATTGGATGGAGATTCTAAAGCTCTTCTCTCATTTGTTAGTGGCTTCAACAAAGTAGTCCTTGATGTGCATTACTACAATATGTTTTGGGACAAATTCAATGGCATGAATGTTCAACAAAATATTGACTTCATAAGAAATGAAAGAGCCTCAGATCTTGCTGGTGTATCTTCAACAAATGCTCTTACTTTTGTTGGTAAAAACCAAATCCTAACCAATTTTATTGGTATCTATATGTGTGTGATAGGGATGAAATGTGATTTAATACATATATGAAATATGACAAAGGCTAACTTGTGCCTTCAAGGGCACATGTTAAGGAACTCATCATAGAAATGTACATATCAAAAATCGCGCATTCAACTTCTTAAAAGTTAACATATTATGTTTTCCAATGCAAAGTTGCTCTTTTTGAGTTGCTTAACATGTGTCATAAGGGCACAAGTTAGTATGACCCATGAAATATATACTAACTCATCCATATTATATATCGGGGATAACTATATGTTTTTTCTTTTTGTAGGGGAATGGACGGGTGAGTGGACCATACAAAGTGCGTCTAAGCAAGATTTCCAAAACTTTGCCCAAGCTCAATTGGATGTGTATTCTCGGGCAACATTTGGATGGGCTTATTGGTCCTACAAATGCCAGTTTAATCGTTGGAGTCTCAAGTGGATGATCGAGAATGGTTACATAAAGTTGAGTAAAAAACATCTACCAACAGATAGCTTTCGACAGGATGTGAGGGATTTCGAGTAA

Protein sequence

>MS.gene56249.t1

MKVIVDLHAIEGSQNGNEHSGTRDGYIEWGDSYIPQTVAVINFLAQRYGNKPSLGGIELMNEPQGVNLDSLKKYYKAAYDAVRKYKPEAYVIMSNPLDGDSKALLSFVSGFNKVVLDVHYYNMFWDKFNGMNVQQNIDFIRNERASDLAGVSSTNALTFVGEWTGEWTIQSASKQDFQNFAQAQLDVYSRATFGWAYWSYKCQFNRWSLKWMIENGYIKLSKKHLPTDSFRQDVRDFE