AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56404


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56404.t1 MTR_4g084280 97.093 172 5 0 1 172 1 172 6.01e-123 343
MS.gene56404.t1 MTR_1g031650 66.871 163 53 1 3 164 4 166 1.59e-76 226
MS.gene56404.t1 MTR_3g067585 54.601 163 74 0 1 163 1 163 4.46e-64 194
MS.gene56404.t1 MTR_1g031650 69.531 128 39 0 37 164 2 129 4.24e-62 188
MS.gene56404.t1 MTR_8g107340 51.220 164 80 0 1 164 17 180 7.32e-58 179
MS.gene56404.t1 MTR_2g011590 51.235 162 79 0 3 164 2 163 1.75e-57 177
MS.gene56404.t1 MTR_6g069470 49.390 164 83 0 1 164 13 176 9.60e-56 174
MS.gene56404.t1 MTR_8g088320 40.000 160 95 1 1 159 161 320 4.07e-35 125
MS.gene56404.t1 MTR_2g030230 38.889 162 94 3 1 159 168 327 5.29e-32 117
MS.gene56404.t1 MTR_3g017500 37.952 166 94 2 1 161 209 370 7.66e-31 115
MS.gene56404.t1 MTR_8g038400 37.654 162 100 1 1 161 206 367 1.72e-30 114
MS.gene56404.t1 MTR_3g017500 37.952 166 94 2 1 161 239 400 2.19e-30 114
MS.gene56404.t1 MTR_2g027100 38.158 152 92 1 1 150 1 152 2.11e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56404.t1 AT1G70810 55.488 164 73 0 1 164 1 164 2.49e-63 192
MS.gene56404.t1 AT1G48590 55.090 167 72 1 1 167 6 169 1.16e-61 188
MS.gene56404.t1 AT1G48590 55.090 167 72 1 1 167 6 169 1.16e-61 188
MS.gene56404.t1 AT1G48590 55.090 167 72 1 1 167 37 200 1.41e-61 189
MS.gene56404.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene56404.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene56404.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene56404.t1 AT3G17980 51.829 164 79 0 1 164 13 176 1.29e-58 181
MS.gene56404.t1 AT1G23140 51.852 162 78 0 1 162 1 162 3.19e-58 179
MS.gene56404.t1 AT1G73580 48.780 164 84 0 1 164 4 167 5.68e-58 179
MS.gene56404.t1 AT2G01540 53.659 164 69 2 5 166 6 164 1.10e-57 179
MS.gene56404.t1 AT1G70800 50.955 157 77 0 1 157 10 166 2.88e-55 172
MS.gene56404.t1 AT1G66360 45.402 174 93 1 1 172 1 174 2.79e-53 167
MS.gene56404.t1 AT5G37740 46.707 167 86 1 1 164 1 167 1.24e-51 163
MS.gene56404.t1 AT5G37740 44.068 177 86 2 1 164 1 177 3.37e-48 154
MS.gene56404.t1 AT1G70790 55.385 130 56 1 39 166 3 132 3.51e-46 148
MS.gene56404.t1 AT1G48590 45.509 167 56 2 1 167 37 168 3.10e-43 141
MS.gene56404.t1 AT4G05330 41.875 160 88 3 1 157 140 297 2.75e-34 122
MS.gene56404.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56404.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56404.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56404.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56404.t1 AT4G05330 41.875 160 88 3 1 157 175 332 1.01e-33 122
MS.gene56404.t1 AT4G05330 41.875 160 88 3 1 157 175 332 1.01e-33 122
MS.gene56404.t1 AT3G07940 39.506 162 97 1 1 161 224 385 2.78e-32 119
MS.gene56404.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene56404.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene56404.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene56404.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111

Find 36 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATCTTAAACTCATTTCT+TGG 0.237962 4.4:+28606870 None:intergenic
TTTCACTGTGGACGACAAAA+TGG 0.248123 4.4:-28607047 MS.gene56404:CDS
GTCAGTTCTTCATTCCATTC+AGG 0.282274 4.4:+28607881 None:intergenic
ATCTTCACACATTGGAGATA+TGG 0.313339 4.4:+28607001 None:intergenic
GTTTCGATGGCAAACATTCT+AGG 0.356912 4.4:-28608271 None:intergenic
GCTAGGCAATTAGTCGTATC+TGG 0.362744 4.4:+28606932 None:intergenic
GTCTTGACTACATGTCCATC+TGG 0.383427 4.4:+28606962 None:intergenic
CTCGGATAAGCCTTTGCCTT+CGG 0.389439 4.4:+28606790 None:intergenic
TCCATGGTGACAAGAACATA+AGG 0.466228 4.4:+28608184 None:intergenic
CCATACGCAGCTACTCTCTT+CGG 0.489124 4.4:+28606910 None:intergenic
CTAACTAACTTGTTCTTCCA+TGG 0.492512 4.4:+28608168 None:intergenic
AGTAGCTGCGTATGGAGAGA+TGG 0.493455 4.4:-28606902 MS.gene56404:CDS
AAGATAACTGCCATCCTGAA+TGG 0.495110 4.4:-28607895 MS.gene56404:CDS
TGTTAACACACCAATACATC+TGG 0.500731 4.4:-28607843 MS.gene56404:intron
TTCACTGTGGACGACAAAAT+GGG 0.505157 4.4:-28607046 MS.gene56404:CDS
ATATCTCCAATGTGTGAAGA+TGG 0.506001 4.4:-28606999 MS.gene56404:CDS
TGAGTACTGACCAGATGTAT+TGG 0.524936 4.4:+28607833 None:intergenic
TATCTCCAATGTGTGAAGAT+GGG 0.525049 4.4:-28606998 MS.gene56404:CDS
CCGAAGAGAGTAGCTGCGTA+TGG 0.539890 4.4:-28606910 MS.gene56404:CDS
GACAAGAACATAAGGGTCGC+TGG 0.578982 4.4:+28608192 None:intergenic
AGATTGAGAAATGTTGACTC+TGG 0.584076 4.4:-28606854 MS.gene56404:CDS
CCCTTATGTTCTTGTCACCA+TGG 0.587591 4.4:-28608185 MS.gene56404:CDS
CCATGGTGACAAGAACATAA+GGG 0.590675 4.4:+28608185 None:intergenic
CGCAGCTACTCTCTTCGGCT+AGG 0.597984 4.4:+28606915 None:intergenic
GTTACAGAATCCGAAGGCAA+AGG 0.605128 4.4:-28606800 MS.gene56404:CDS
AAGTACTAGTCGAAATTGAG+TGG 0.607422 4.4:-28606829 MS.gene56404:CDS
TCTTGACTACATGTCCATCT+GGG 0.611492 4.4:+28606963 None:intergenic
GGGTTGAGCGATCTCCCAGA+TGG 0.612527 4.4:-28606977 MS.gene56404:CDS
GTTCTTCATTCCATTCAGGA+TGG 0.627122 4.4:+28607885 None:intergenic
ATCTCCAATGTGTGAAGATG+GGG 0.633945 4.4:-28606997 MS.gene56404:CDS
TCAACCCCATCTTCACACAT+TGG 0.658196 4.4:+28606993 None:intergenic
CGACAAAGACACTTTCACTG+TGG 0.661032 4.4:-28607059 MS.gene56404:CDS
GTACTCCTAAAACTCAACCT+CGG 0.669415 4.4:+28606772 None:intergenic
ATTGATGTTACAGAATCCGA+AGG 0.687927 4.4:-28606806 MS.gene56404:CDS
CGAAGGCAAAGGCTTATCCG+AGG 0.695987 4.4:-28606789 MS.gene56404:CDS
TCGCTGGTACGAGAATCATG+AGG 0.744769 4.4:+28608208 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAAAAGGAAAAA+TGG - chr4.4:28607278-28607297 MS.gene56404:intron 10.0%
!! AAATAAATAAAAGGAAAAAT+GGG - chr4.4:28607279-28607298 MS.gene56404:intron 10.0%
!! CAAAAGAGAAAATAAATAAA+AGG - chr4.4:28607270-28607289 MS.gene56404:intron 15.0%
!! TTATGATATGTTACTATAAA+TGG - chr4.4:28607098-28607117 MS.gene56404:intron 15.0%
!! TTTGAATTTAAATTAACCAT+AGG - chr4.4:28607658-28607677 MS.gene56404:intron 15.0%
!!! AATCTTAGAAATTTTATCTT+AGG + chr4.4:28607526-28607545 None:intergenic 15.0%
!!! ATCTTAGAAATTTTATCTTA+GGG + chr4.4:28607525-28607544 None:intergenic 15.0%
!!! TTATCTTAGAAATTTTATCT+CGG + chr4.4:28607496-28607515 None:intergenic 15.0%
!! ATAGTAGTCTAATAAATTAG+AGG + chr4.4:28607358-28607377 None:intergenic 20.0%
!!! ATCAACATAAAATACTGATT+TGG + chr4.4:28606961-28606980 None:intergenic 20.0%
!!! TCAATTTGAAACGAAAAAAA+CGG + chr4.4:28607905-28607924 None:intergenic 20.0%
! AAATTAACCATAGGATCAAA+GGG - chr4.4:28607667-28607686 MS.gene56404:intron 25.0%
! ACCACCAACAATTTAATATT+AGG + chr4.4:28607932-28607951 None:intergenic 25.0%
! ATGAGAATCACAAACAAATA+CGG - chr4.4:28607810-28607829 MS.gene56404:intron 25.0%
! TAAATTAACCATAGGATCAA+AGG - chr4.4:28607666-28607685 MS.gene56404:intron 25.0%
! TCAATCTTAAACTCATTTCT+TGG + chr4.4:28608176-28608195 None:intergenic 25.0%
! TCTAAGAACTGATTGAAATT+TGG - chr4.4:28606982-28607001 MS.gene56404:CDS 25.0%
!! ACTAAGATCTTTCTTTTTAC+TGG - chr4.4:28607041-28607060 MS.gene56404:CDS 25.0%
!!! AATTTTTTATGCGTTTCCTT+TGG - chr4.4:28607399-28607418 MS.gene56404:intron 25.0%
AAGTAATGTAGCCTAACAAA+TGG - chr4.4:28607758-28607777 MS.gene56404:intron 30.0%
ATCACACAAGTATAAACCAA+AGG + chr4.4:28607418-28607437 None:intergenic 30.0%
ATCAGACTTCGTATCAAAAA+AGG - chr4.4:28606799-28606818 MS.gene56404:CDS 30.0%
CGAGCCTAATATTAAATTGT+TGG - chr4.4:28607925-28607944 MS.gene56404:intron 30.0%
CTCAGTTGAAGATAAGTTTA+AGG + chr4.4:28607557-28607576 None:intergenic 30.0%
GCCTAATATTAAATTGTTGG+TGG - chr4.4:28607928-28607947 MS.gene56404:intron 30.0%
TGGTTTATACTTGTGTGATT+TGG - chr4.4:28607419-28607438 MS.gene56404:intron 30.0%
TGTGATTTAAGCCATTTGTT+AGG + chr4.4:28607772-28607791 None:intergenic 30.0%
TTCTGCAACTTAAACAAAGT+TGG + chr4.4:28607240-28607259 None:intergenic 30.0%
AACTGAGTTCATCACAAACT+TGG - chr4.4:28607690-28607709 MS.gene56404:intron 35.0%
AAGTACTAGTCGAAATTGAG+TGG - chr4.4:28608214-28608233 MS.gene56404:CDS 35.0%
ATATCTCCAATGTGTGAAGA+TGG - chr4.4:28608044-28608063 MS.gene56404:intron 35.0%
ATCTTCACACATTGGAGATA+TGG + chr4.4:28608045-28608064 None:intergenic 35.0%
ATTGATGTTACAGAATCCGA+AGG - chr4.4:28608237-28608256 MS.gene56404:CDS 35.0%
CATATCGTTGTCCAAAAACT+TGG - chr4.4:28607605-28607624 MS.gene56404:intron 35.0%
TATCTCCAATGTGTGAAGAT+GGG - chr4.4:28608045-28608064 MS.gene56404:intron 35.0%
TGTTAACACACCAATACATC+TGG - chr4.4:28607200-28607219 MS.gene56404:intron 35.0%
!! AGATTGAGAAATGTTGACTC+TGG - chr4.4:28608189-28608208 MS.gene56404:CDS 35.0%
!! CAAGTTTTTGGACAACGATA+TGG + chr4.4:28607607-28607626 None:intergenic 35.0%
!! CTAACTAACTTGTTCTTCCA+TGG + chr4.4:28606878-28606897 None:intergenic 35.0%
AAGATAACTGCCATCCTGAA+TGG - chr4.4:28607148-28607167 MS.gene56404:intron 40.0%
ATACGGCTGATGCATTGTAT+TGG - chr4.4:28607827-28607846 MS.gene56404:intron 40.0%
ATCTCCAATGTGTGAAGATG+GGG - chr4.4:28608046-28608065 MS.gene56404:intron 40.0%
CCATGGTGACAAGAACATAA+GGG + chr4.4:28606861-28606880 None:intergenic 40.0%
GATGTTGTAGCCTAGATGAA+GGG - chr4.4:28607869-28607888 MS.gene56404:CDS 40.0%
GTCAGTTCTTCATTCCATTC+AGG + chr4.4:28607165-28607184 None:intergenic 40.0%
GTTCATCACAAACTTGGTTG+CGG - chr4.4:28607696-28607715 MS.gene56404:intron 40.0%
GTTCTTCATTCCATTCAGGA+TGG + chr4.4:28607161-28607180 None:intergenic 40.0%
TCCATGGTGACAAGAACATA+AGG + chr4.4:28606862-28606881 None:intergenic 40.0%
TCTTGACTACATGTCCATCT+GGG + chr4.4:28608083-28608102 None:intergenic 40.0%
TGATGTTGTAGCCTAGATGA+AGG - chr4.4:28607868-28607887 MS.gene56404:CDS 40.0%
TTCACTGTGGACGACAAAAT+GGG - chr4.4:28607997-28608016 MS.gene56404:intron 40.0%
TTTCACTGTGGACGACAAAA+TGG - chr4.4:28607996-28608015 MS.gene56404:intron 40.0%
!! TGAGTACTGACCAGATGTAT+TGG + chr4.4:28607213-28607232 None:intergenic 40.0%
CCCTTATGTTCTTGTCACCA+TGG - chr4.4:28606858-28606877 MS.gene56404:CDS 45.0%
CGACAAAGACACTTTCACTG+TGG - chr4.4:28607984-28608003 MS.gene56404:intron 45.0%
CTCAGTTCCCTTTGATCCTA+TGG + chr4.4:28607677-28607696 None:intergenic 45.0%
GCTAGGCAATTAGTCGTATC+TGG + chr4.4:28608114-28608133 None:intergenic 45.0%
GTCTTGACTACATGTCCATC+TGG + chr4.4:28608084-28608103 None:intergenic 45.0%
GTTACAGAATCCGAAGGCAA+AGG - chr4.4:28608243-28608262 MS.gene56404:CDS 45.0%
TCAACCCCATCTTCACACAT+TGG + chr4.4:28608053-28608072 None:intergenic 45.0%
! GTAGACTCTCGCCAAGTTTT+TGG + chr4.4:28607619-28607638 None:intergenic 45.0%
AGTAGCTGCGTATGGAGAGA+TGG - chr4.4:28608141-28608160 MS.gene56404:intron 50.0%
CCATACGCAGCTACTCTCTT+CGG + chr4.4:28608136-28608155 None:intergenic 50.0%
CTCGGATAAGCCTTTGCCTT+CGG + chr4.4:28608256-28608275 None:intergenic 50.0%
GACAAGAACATAAGGGTCGC+TGG + chr4.4:28606854-28606873 None:intergenic 50.0%
TCGCTGGTACGAGAATCATG+AGG + chr4.4:28606838-28606857 None:intergenic 50.0%
TCTGTGTGCACCCTTCATCT+AGG + chr4.4:28607882-28607901 None:intergenic 50.0%
CCGAAGAGAGTAGCTGCGTA+TGG - chr4.4:28608133-28608152 MS.gene56404:intron 55.0%
CGAAGGCAAAGGCTTATCCG+AGG - chr4.4:28608254-28608273 MS.gene56404:CDS 55.0%
CGCAGCTACTCTCTTCGGCT+AGG + chr4.4:28608131-28608150 None:intergenic 60.0%
GGGTTGAGCGATCTCCCAGA+TGG - chr4.4:28608066-28608085 MS.gene56404:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 28606778 28608287 28606778 ID=MS.gene56404
chr4.4 mRNA 28606778 28608287 28606778 ID=MS.gene56404.t1;Parent=MS.gene56404
chr4.4 exon 28608177 28608287 28608177 ID=MS.gene56404.t1.exon1;Parent=MS.gene56404.t1
chr4.4 CDS 28608177 28608287 28608177 ID=cds.MS.gene56404.t1;Parent=MS.gene56404.t1
chr4.4 exon 28607844 28607939 28607844 ID=MS.gene56404.t1.exon2;Parent=MS.gene56404.t1
chr4.4 CDS 28607844 28607939 28607844 ID=cds.MS.gene56404.t1;Parent=MS.gene56404.t1
chr4.4 exon 28606778 28607089 28606778 ID=MS.gene56404.t1.exon3;Parent=MS.gene56404.t1
chr4.4 CDS 28606778 28607089 28606778 ID=cds.MS.gene56404.t1;Parent=MS.gene56404.t1
Gene Sequence

>MS.gene56404

ATGGCAAACATTCTAGGTCTTATCAGACTTCGTATCAAAAAAGGCACTAATCTCATACCTCATGATTCTCGTACCAGCGACCCTTATGTTCTTGTCACCATGGAAGAACAAGTTAGTTAGCTAGATGCTATTTGTATTTATTTTATTTTATTTTATTTTATTTCATATTTGAAATATTAACCAAATCAGTATTTTATGTTGATGTCTAAGAACTGATTGAAATTTGGATTACATGTTTAACATAATTAATAACATAAAGTTGAACTAAGATCTTTCTTTTTACTGGTATAGTAAATATAAATCTGTGATTAATTTAACTGTTATGATATGTTACTATAAATGGTGCAGACACTGAAGACTGCTGTTGTGAAAGATAACTGCCATCCTGAATGGAATGAAGAACTGACACTTTACATTAAAGATGTTAACACACCAATACATCTGGTCAGTACTCAAACTCCAACTTTGTTTAAGTTGCAGAAGAATTTATCTCAAAAGAGAAAATAAATAAAAGGAAAAATGGGAAACATATATAAAGAAGAATAAAACTAAAATTAGCTACACAAATTTATGACTACCTCTAATTTATTAGACTACTATTGTATTGTTTTGCTTTTAAAGAATTTTTTATGCGTTTCCTTTGGTTTATACTTGTGTGATTTGGATGTAAACTTAAGTCAGTTTTTAAAGTTTTAATGTAATGAATGCACAATTTCCGAGATAAAATTTCTAAGATAACAAGTGCCCTAAGATAAAATTTCTAAGATTAGCATTTTCCTTAAACTTATCTTCAACTGAGATAGTGTCATGTCATTATCTAAATCGACCATATCGTTGTCCAAAAACTTGGCGAGAGTCTACATTCATTAAAACATTGAGATTTGAATTTAAATTAACCATAGGATCAAAGGGAACTGAGTTCATCACAAACTTGGTTGCGGCGCTTACATCACTGAAACTCTCTATCTTATGTCATTAGTAAGTAATGTAGCCTAACAAATGGCTTAAATCACATTGTGATCTTTGATATCAATGAGAATCACAAACAAATACGGCTGATGCATTGTATTGGCGAAGACATAAAAAGTCTTGATGTTGTAGCCTAGATGAAGGGTGCACACAGACCGTTTTTTTCGTTTCAAATTGACGAGCCTAATATTAAATTGTTGGTGGTATTGAATTTTGTTTTTCTATTCAGACAGTTTGCGACAAAGACACTTTCACTGTGGACGACAAAATGGGAGAAGCAGACATAGACATAAAACCATATCTCCAATGTGTGAAGATGGGGTTGAGCGATCTCCCAGATGGACATGTAGTCAAGACTGTTCAACCAGATACGACTAATTGCCTAGCCGAAGAGAGTAGCTGCGTATGGAGAGATGGAAAAGTTGTCCAAGAAATGAGTTTAAGATTGAGAAATGTTGACTCTGGTGAAGTACTAGTCGAAATTGAGTGGATTGATGTTACAGAATCCGAAGGCAAAGGCTTATCCGAGGTTGAGTTTTAG

Protein sequence

>MS.gene56404.t1

MANILGLIRLRIKKGTNLIPHDSRTSDPYVLVTMEEQTLKTAVVKDNCHPEWNEELTLYIKDVNTPIHLTVCDKDTFTVDDKMGEADIDIKPYLQCVKMGLSDLPDGHVVKTVQPDTTNCLAEESSCVWRDGKVVQEMSLRLRNVDSGEVLVEIEWIDVTESEGKGLSEVEF