AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059511


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059511.t1 MTR_4g084280 97.093 172 5 0 1 172 1 172 6.01e-123 343
MS.gene059511.t1 MTR_1g031650 66.871 163 53 1 3 164 4 166 1.59e-76 226
MS.gene059511.t1 MTR_3g067585 54.601 163 74 0 1 163 1 163 4.46e-64 194
MS.gene059511.t1 MTR_1g031650 69.531 128 39 0 37 164 2 129 4.24e-62 188
MS.gene059511.t1 MTR_8g107340 51.220 164 80 0 1 164 17 180 7.32e-58 179
MS.gene059511.t1 MTR_2g011590 51.235 162 79 0 3 164 2 163 1.75e-57 177
MS.gene059511.t1 MTR_6g069470 49.390 164 83 0 1 164 13 176 9.60e-56 174
MS.gene059511.t1 MTR_8g088320 40.000 160 95 1 1 159 161 320 4.07e-35 125
MS.gene059511.t1 MTR_2g030230 38.889 162 94 3 1 159 168 327 5.29e-32 117
MS.gene059511.t1 MTR_3g017500 37.952 166 94 2 1 161 209 370 7.66e-31 115
MS.gene059511.t1 MTR_8g038400 37.654 162 100 1 1 161 206 367 1.72e-30 114
MS.gene059511.t1 MTR_3g017500 37.952 166 94 2 1 161 239 400 2.19e-30 114
MS.gene059511.t1 MTR_2g027100 38.158 152 92 1 1 150 1 152 2.11e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059511.t1 AT1G70810 55.488 164 73 0 1 164 1 164 2.49e-63 192
MS.gene059511.t1 AT1G48590 55.090 167 72 1 1 167 6 169 1.16e-61 188
MS.gene059511.t1 AT1G48590 55.090 167 72 1 1 167 6 169 1.16e-61 188
MS.gene059511.t1 AT1G48590 55.090 167 72 1 1 167 37 200 1.41e-61 189
MS.gene059511.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene059511.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene059511.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene059511.t1 AT3G17980 51.829 164 79 0 1 164 13 176 1.29e-58 181
MS.gene059511.t1 AT1G23140 51.852 162 78 0 1 162 1 162 3.19e-58 179
MS.gene059511.t1 AT1G73580 48.780 164 84 0 1 164 4 167 5.68e-58 179
MS.gene059511.t1 AT2G01540 53.659 164 69 2 5 166 6 164 1.10e-57 179
MS.gene059511.t1 AT1G70800 50.955 157 77 0 1 157 10 166 2.88e-55 172
MS.gene059511.t1 AT1G66360 45.402 174 93 1 1 172 1 174 2.79e-53 167
MS.gene059511.t1 AT5G37740 46.707 167 86 1 1 164 1 167 1.24e-51 163
MS.gene059511.t1 AT5G37740 44.068 177 86 2 1 164 1 177 3.37e-48 154
MS.gene059511.t1 AT1G70790 55.385 130 56 1 39 166 3 132 3.51e-46 148
MS.gene059511.t1 AT1G48590 45.509 167 56 2 1 167 37 168 3.10e-43 141
MS.gene059511.t1 AT4G05330 41.875 160 88 3 1 157 140 297 2.75e-34 122
MS.gene059511.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene059511.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene059511.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene059511.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene059511.t1 AT4G05330 41.875 160 88 3 1 157 175 332 1.01e-33 122
MS.gene059511.t1 AT4G05330 41.875 160 88 3 1 157 175 332 1.01e-33 122
MS.gene059511.t1 AT3G07940 39.506 162 97 1 1 161 224 385 2.78e-32 119
MS.gene059511.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene059511.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene059511.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene059511.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111

Find 36 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATCTTAAACTCATTTCT+TGG 0.237962 4.4:-27520122 None:intergenic
TTTCACTGTGGACGACAAAA+TGG 0.248123 4.4:+27519945 MS.gene059511:CDS
GTCAGTTCTTCATTCCATTC+AGG 0.282274 4.4:-27519118 None:intergenic
ATCTTCACACATTGGAGATA+TGG 0.313339 4.4:-27519991 None:intergenic
GTTTCGATGGCAAACATTCT+AGG 0.356912 4.4:+27518731 None:intergenic
GCTAGGCAATTAGTCGTATC+TGG 0.362744 4.4:-27520060 None:intergenic
GTCTTGACTACATGTCCATC+TGG 0.383427 4.4:-27520030 None:intergenic
CTCGGATAAGCCTTTGCCTT+CGG 0.389439 4.4:-27520202 None:intergenic
TCCATGGTGACAAGAACATA+AGG 0.466228 4.4:-27518818 None:intergenic
CCATACGCAGCTACTCTCTT+CGG 0.489124 4.4:-27520082 None:intergenic
CTAACTAACTTGTTCTTCCA+TGG 0.492512 4.4:-27518834 None:intergenic
AGTAGCTGCGTATGGAGAGA+TGG 0.493455 4.4:+27520090 MS.gene059511:CDS
AAGATAACTGCCATCCTGAA+TGG 0.495110 4.4:+27519104 MS.gene059511:CDS
TGTTAACACACCAATACATC+TGG 0.500731 4.4:+27519156 MS.gene059511:CDS
TTCACTGTGGACGACAAAAT+GGG 0.505157 4.4:+27519946 MS.gene059511:CDS
ATATCTCCAATGTGTGAAGA+TGG 0.506001 4.4:+27519993 MS.gene059511:CDS
TGAGTACTGACCAGATGTAT+TGG 0.524936 4.4:-27519166 None:intergenic
TATCTCCAATGTGTGAAGAT+GGG 0.525049 4.4:+27519994 MS.gene059511:CDS
CCGAAGAGAGTAGCTGCGTA+TGG 0.539890 4.4:+27520082 MS.gene059511:CDS
GACAAGAACATAAGGGTCGC+TGG 0.578982 4.4:-27518810 None:intergenic
AGATTGAGAAATGTTGACTC+TGG 0.584076 4.4:+27520138 MS.gene059511:CDS
CCCTTATGTTCTTGTCACCA+TGG 0.587591 4.4:+27518817 MS.gene059511:CDS
CCATGGTGACAAGAACATAA+GGG 0.590675 4.4:-27518817 None:intergenic
CGCAGCTACTCTCTTCGGCT+AGG 0.597984 4.4:-27520077 None:intergenic
GTTACAGAATCCGAAGGCAA+AGG 0.605128 4.4:+27520192 MS.gene059511:CDS
AAGTACTAGTCGAAATTGAG+TGG 0.607422 4.4:+27520163 MS.gene059511:CDS
TCTTGACTACATGTCCATCT+GGG 0.611492 4.4:-27520029 None:intergenic
GGGTTGAGCGATCTCCCAGA+TGG 0.612527 4.4:+27520015 MS.gene059511:CDS
GTTCTTCATTCCATTCAGGA+TGG 0.627122 4.4:-27519114 None:intergenic
ATCTCCAATGTGTGAAGATG+GGG 0.633945 4.4:+27519995 MS.gene059511:CDS
TCAACCCCATCTTCACACAT+TGG 0.658196 4.4:-27519999 None:intergenic
CGACAAAGACACTTTCACTG+TGG 0.661032 4.4:+27519933 MS.gene059511:CDS
GTACTCCTAAAACTCAACCT+CGG 0.669415 4.4:-27520220 None:intergenic
ATTGATGTTACAGAATCCGA+AGG 0.687927 4.4:+27520186 MS.gene059511:CDS
CGAAGGCAAAGGCTTATCCG+AGG 0.695987 4.4:+27520203 MS.gene059511:CDS
TCGCTGGTACGAGAATCATG+AGG 0.744769 4.4:-27518794 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAAAAGGAAAAA+TGG + chr4.4:27519234-27519253 MS.gene059511:intron 10.0%
!! AAATAAATAAAAGGAAAAAT+GGG + chr4.4:27519235-27519254 MS.gene059511:intron 10.0%
!! CAAAAGAGAAAATAAATAAA+AGG + chr4.4:27519226-27519245 MS.gene059511:intron 15.0%
!! TTATGATATGTTACTATAAA+TGG + chr4.4:27519054-27519073 MS.gene059511:intron 15.0%
!!! AATCTTAGAAATTTTATCTT+AGG - chr4.4:27519482-27519501 None:intergenic 15.0%
!!! ATCTTAGAAATTTTATCTTA+GGG - chr4.4:27519481-27519500 None:intergenic 15.0%
!!! TTATCTTAGAAATTTTATCT+CGG - chr4.4:27519452-27519471 None:intergenic 15.0%
!! ATAGTAGTCTAATAAATTAG+AGG - chr4.4:27519314-27519333 None:intergenic 20.0%
!!! ACTAAGATCTTTCTTTTTAT+TGG + chr4.4:27518995-27519014 MS.gene059511:intron 20.0%
!!! ATCAACATAAAATACTGATT+TGG - chr4.4:27518915-27518934 None:intergenic 20.0%
!!! TCAATTTGAAACGAAAAAAA+CGG - chr4.4:27519854-27519873 None:intergenic 20.0%
! ACCACCAACAATTTAATATT+AGG - chr4.4:27519881-27519900 None:intergenic 25.0%
! ATGAGAATCACAAACAAATA+CGG + chr4.4:27519759-27519778 MS.gene059511:intron 25.0%
! ATTGAGATTTGAATAACCAT+AGG + chr4.4:27519607-27519626 MS.gene059511:intron 25.0%
! TCAATCTTAAACTCATTTCT+TGG - chr4.4:27520125-27520144 None:intergenic 25.0%
! TTCTGCAACTTAAAAAAAGT+TGG - chr4.4:27519196-27519215 None:intergenic 25.0%
!!! AATTTTTTATGCGTTTCCTT+TGG + chr4.4:27519355-27519374 MS.gene059511:intron 25.0%
AAGTAATGTAGCCTAACAAA+TGG + chr4.4:27519707-27519726 MS.gene059511:intron 30.0%
ATCACACAAGTATAAACCAA+AGG - chr4.4:27519374-27519393 None:intergenic 30.0%
ATCAGACTTCGTATCAAAAA+AGG + chr4.4:27518758-27518777 MS.gene059511:CDS 30.0%
CGAGCCTAATATTAAATTGT+TGG + chr4.4:27519874-27519893 MS.gene059511:intron 30.0%
CTCAGTTGAAGATAAGTTTA+AGG - chr4.4:27519513-27519532 None:intergenic 30.0%
GCCTAATATTAAATTGTTGG+TGG + chr4.4:27519877-27519896 MS.gene059511:intron 30.0%
TCCAAATTTCAATCAGTTCT+TGG - chr4.4:27518940-27518959 None:intergenic 30.0%
TCCAAGAACTGATTGAAATT+TGG + chr4.4:27518936-27518955 MS.gene059511:intron 30.0%
TGAATAACCATAGGATCAAA+GGG + chr4.4:27519616-27519635 MS.gene059511:intron 30.0%
TGGTTTATACTTGTGTGATT+TGG + chr4.4:27519375-27519394 MS.gene059511:intron 30.0%
TGTGATTTAAGCCATTTGTT+AGG - chr4.4:27519721-27519740 None:intergenic 30.0%
TTGAATAACCATAGGATCAA+AGG + chr4.4:27519615-27519634 MS.gene059511:intron 30.0%
AACTGAGTTCATCACAAACT+TGG + chr4.4:27519639-27519658 MS.gene059511:intron 35.0%
AAGTACTAGTCGAAATTGAG+TGG + chr4.4:27520163-27520182 MS.gene059511:CDS 35.0%
ATATCTCCAATGTGTGAAGA+TGG + chr4.4:27519993-27520012 MS.gene059511:CDS 35.0%
ATCTTCACACATTGGAGATA+TGG - chr4.4:27519994-27520013 None:intergenic 35.0%
ATTGATGTTACAGAATCCGA+AGG + chr4.4:27520186-27520205 MS.gene059511:CDS 35.0%
CATATCGTTGTCCAAAAACT+TGG + chr4.4:27519561-27519580 MS.gene059511:intron 35.0%
TATCTCCAATGTGTGAAGAT+GGG + chr4.4:27519994-27520013 MS.gene059511:CDS 35.0%
TGTTAACACACCAATACATC+TGG + chr4.4:27519156-27519175 MS.gene059511:CDS 35.0%
!! AGATTGAGAAATGTTGACTC+TGG + chr4.4:27520138-27520157 MS.gene059511:CDS 35.0%
!! CAAGTTTTTGGACAACGATA+TGG - chr4.4:27519563-27519582 None:intergenic 35.0%
!! CTAACTAACTTGTTCTTCCA+TGG - chr4.4:27518837-27518856 None:intergenic 35.0%
AAGATAACTGCCATCCTGAA+TGG + chr4.4:27519104-27519123 MS.gene059511:CDS 40.0%
ATCTCCAATGTGTGAAGATG+GGG + chr4.4:27519995-27520014 MS.gene059511:CDS 40.0%
CCATGGTGACAAGAACATAA+GGG - chr4.4:27518820-27518839 None:intergenic 40.0%
GATGTTGTAGCCTAGATGAA+GGG + chr4.4:27519818-27519837 MS.gene059511:intron 40.0%
GTCAGTTCTTCATTCCATTC+AGG - chr4.4:27519121-27519140 None:intergenic 40.0%
GTTCATCACAAACTTGGTTG+CGG + chr4.4:27519645-27519664 MS.gene059511:intron 40.0%
GTTCTTCATTCCATTCAGGA+TGG - chr4.4:27519117-27519136 None:intergenic 40.0%
TCCATGGTGACAAGAACATA+AGG - chr4.4:27518821-27518840 None:intergenic 40.0%
TCTTGACTACATGTCCATCT+GGG - chr4.4:27520032-27520051 None:intergenic 40.0%
TGATGTTGTAGCCTAGATGA+AGG + chr4.4:27519817-27519836 MS.gene059511:intron 40.0%
TTCACTGTGGACGACAAAAT+GGG + chr4.4:27519946-27519965 MS.gene059511:CDS 40.0%
TTTCACTGTGGACGACAAAA+TGG + chr4.4:27519945-27519964 MS.gene059511:CDS 40.0%
!! TGAGTACTGACCAGATGTAT+TGG - chr4.4:27519169-27519188 None:intergenic 40.0%
CCCTTATGTTCTTGTCACCA+TGG + chr4.4:27518817-27518836 MS.gene059511:CDS 45.0%
CGACAAAGACACTTTCACTG+TGG + chr4.4:27519933-27519952 MS.gene059511:CDS 45.0%
CTCAGTTCCCTTTGATCCTA+TGG - chr4.4:27519626-27519645 None:intergenic 45.0%
GCTAGGCAATTAGTCGTATC+TGG - chr4.4:27520063-27520082 None:intergenic 45.0%
GTCTTGACTACATGTCCATC+TGG - chr4.4:27520033-27520052 None:intergenic 45.0%
GTTACAGAATCCGAAGGCAA+AGG + chr4.4:27520192-27520211 MS.gene059511:CDS 45.0%
TCAACCCCATCTTCACACAT+TGG - chr4.4:27520002-27520021 None:intergenic 45.0%
! GTAGACTCTCGCCAAGTTTT+TGG - chr4.4:27519575-27519594 None:intergenic 45.0%
AGTAGCTGCGTATGGAGAGA+TGG + chr4.4:27520090-27520109 MS.gene059511:CDS 50.0%
CCATACGCAGCTACTCTCTT+CGG - chr4.4:27520085-27520104 None:intergenic 50.0%
CTCGGATAAGCCTTTGCCTT+CGG - chr4.4:27520205-27520224 None:intergenic 50.0%
GACAAGAACATAAGGGTCGC+TGG - chr4.4:27518813-27518832 None:intergenic 50.0%
TCGCTGGTACGAGAATCATG+AGG - chr4.4:27518797-27518816 None:intergenic 50.0%
TCTGTGTGCACCCTTCATCT+AGG - chr4.4:27519831-27519850 None:intergenic 50.0%
CCGAAGAGAGTAGCTGCGTA+TGG + chr4.4:27520082-27520101 MS.gene059511:CDS 55.0%
CGAAGGCAAAGGCTTATCCG+AGG + chr4.4:27520203-27520222 MS.gene059511:CDS 55.0%
CGCAGCTACTCTCTTCGGCT+AGG - chr4.4:27520080-27520099 None:intergenic 60.0%
GGGTTGAGCGATCTCCCAGA+TGG + chr4.4:27520015-27520034 MS.gene059511:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 27518737 27520236 27518737 ID=MS.gene059511
chr4.4 mRNA 27518737 27520236 27518737 ID=MS.gene059511.t1;Parent=MS.gene059511
chr4.4 exon 27518737 27518847 27518737 ID=MS.gene059511.t1.exon1;Parent=MS.gene059511.t1
chr4.4 CDS 27518737 27518847 27518737 ID=cds.MS.gene059511.t1;Parent=MS.gene059511.t1
chr4.4 exon 27519082 27519177 27519082 ID=MS.gene059511.t1.exon2;Parent=MS.gene059511.t1
chr4.4 CDS 27519082 27519177 27519082 ID=cds.MS.gene059511.t1;Parent=MS.gene059511.t1
chr4.4 exon 27519925 27520236 27519925 ID=MS.gene059511.t1.exon3;Parent=MS.gene059511.t1
chr4.4 CDS 27519925 27520236 27519925 ID=cds.MS.gene059511.t1;Parent=MS.gene059511.t1
Gene Sequence

>MS.gene059511

ATGGCAAACATTCTAGGTCTTATCAGACTTCGTATCAAAAAAGGCACTAATCTCATACCTCATGATTCTCGTACCAGCGACCCTTATGTTCTTGTCACCATGGAAGAACAAGTTAGTTAGCTAGATGCTATTTGTATTTATTTTATTTTATTTTATTTCATATTTGAAATATTAACCAAATCAGTATTTTATGTTGATGTCCAAGAACTGATTGAAATTTGGATTACATGTTTAACATAATTAATAACATAAAGTTGAACTAAGATCTTTCTTTTTATTGGTATAGTAAATATATAAATCTGTGATTAATTTCACTGTTATGATATGTTACTATAAATGGTGCAGACACTGAAGACTGCTGTTGTGAAAGATAACTGCCATCCTGAATGGAATGAAGAACTGACACTTTACATTAAAGATGTTAACACACCAATACATCTGGTCAGTACTCAAACTCCAACTTTTTTTAAGTTGCAGAAGAATTTATCTCAAAAGAGAAAATAAATAAAAGGAAAAATGGGAAACATATATAAAGAAGAATAAAACTAAAATTAGCTACACAAATTTATGACTACCTCTAATTTATTAGACTACTATTGTATTGTTTTGCTTTTAAAGAATTTTTTATGCGTTTCCTTTGGTTTATACTTGTGTGATTTGGATGTAAACTTAAGTCAGTTTTTAAAGTTTTAATGTAATGAATGCACAATTTCCGAGATAAAATTTCTAAGATAACAAGTGCCCTAAGATAAAATTTCTAAGATTAGCATTTTCCTTAAACTTATCTTCAACTGAGATAGTGTCATGTCATTATCTAAATCGACCATATCGTTGTCCAAAAACTTGGCGAGAGTCTACATTCATTAAAACATTGAGATTTGAATAACCATAGGATCAAAGGGAACTGAGTTCATCACAAACTTGGTTGCGGCGCTTACATCATTGAAACTCTCTATCTTATGTCATTAGTAAGTAATGTAGCCTAACAAATGGCTTAAATCACATTGTGATCTTTGATATCAATGAGAATCACAAACAAATACGGCTGATGCATTGTATTTGCGAAGACATAAAAAGTCTTGATGTTGTAGCCTAGATGAAGGGTGCACACAGACCGTTTTTTTCGTTTCAAATTGACGAGCCTAATATTAAATTGTTGGTGGTATTGAATTTTGTTTTTCTATTCAGACAGTTTGCGACAAAGACACTTTCACTGTGGACGACAAAATGGGAGAAGCAGACATAGACATAAAACCATATCTCCAATGTGTGAAGATGGGGTTGAGCGATCTCCCAGATGGACATGTAGTCAAGACTGTTCAACCAGATACGACTAATTGCCTAGCCGAAGAGAGTAGCTGCGTATGGAGAGATGGAAAAGTCGTCCAAGAAATGAGTTTAAGATTGAGAAATGTTGACTCTGGTGAAGTACTAGTCGAAATTGAGTGGATTGATGTTACAGAATCCGAAGGCAAAGGCTTATCCGAGGTTGAGTTTTAG

Protein sequence

>MS.gene059511.t1

MANILGLIRLRIKKGTNLIPHDSRTSDPYVLVTMEEQTLKTAVVKDNCHPEWNEELTLYIKDVNTPIHLTVCDKDTFTVDDKMGEADIDIKPYLQCVKMGLSDLPDGHVVKTVQPDTTNCLAEESSCVWRDGKVVQEMSLRLRNVDSGEVLVEIEWIDVTESEGKGLSEVEF