AlfalfaGEDB | MS.gene57075

M. sativa cultivar XinJiangDaYe / MS.gene57075

KEGG: -
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57075.t1	XP_013450842.1	96	276	7	1	26	301	113	384	6.30E-151	543.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57075.t1	Q9FL07	58.4	322	99	10	6	306	68	375	5.0e-88	325.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57075.t1	A0A072U5F9	96.0	276	7	1	26	301	113	384	4.5e-151	543.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049647	MS.gene57075	0.81559	8.55E-52	-1.69E-46
MS.gene051700	MS.gene57075	0.804085	2.56E-49	-1.69E-46
MS.gene052434	MS.gene57075	0.804785	1.83E-49	-1.69E-46
MS.gene053080	MS.gene57075	0.801479	8.83E-49	-1.69E-46
MS.gene053226	MS.gene57075	0.804988	1.66E-49	-1.69E-46
MS.gene055603	MS.gene57075	0.804226	2.39E-49	-1.69E-46
MS.gene056403	MS.gene57075	0.805505	1.29E-49	-1.69E-46
MS.gene060365	MS.gene57075	0.815163	1.06E-51	-1.69E-46
MS.gene060586	MS.gene57075	0.816869	4.43E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57075.t1	MTR_6g007737	95.946	296	7	2	6	301	94	384	0.0	585
MS.gene57075.t1	MTR_8g063660	55.197	279	115	3	6	274	28	306	1.19e-92	278
MS.gene57075.t1	MTR_5g034790	56.140	285	111	6	6	283	72	349	5.95e-91	275
MS.gene57075.t1	MTR_4g106680	51.429	280	92	9	6	281	67	306	3.56e-84	256
MS.gene57075.t1	MTR_4g129420	38.871	319	120	12	24	274	95	406	1.25e-50	175
MS.gene57075.t1	MTR_3g100400	53.521	71	33	0	44	114	128	198	4.70e-23	98.6
MS.gene57075.t1	MTR_5g030010	42.453	106	61	0	12	117	88	193	3.44e-22	95.5
MS.gene57075.t1	MTR_5g064890	52.500	80	38	0	46	125	128	207	1.81e-21	93.6
MS.gene57075.t1	MTR_3g110190	46.591	88	45	1	44	131	64	149	3.41e-21	92.4
MS.gene57075.t1	MTR_8g089880	45.882	85	46	0	46	130	127	211	6.71e-21	90.5
MS.gene57075.t1	MTR_4g093830	45.349	86	47	0	39	124	119	204	7.86e-21	91.7
MS.gene57075.t1	MTR_5g066250	48.684	76	39	0	39	114	127	202	1.89e-20	91.3
MS.gene57075.t1	MTR_2g009310	40.517	116	57	2	11	120	100	209	2.75e-20	90.5
MS.gene57075.t1	MTR_4g063070	37.500	136	70	3	5	129	262	393	4.32e-20	90.1
MS.gene57075.t1	MTR_8g066780	43.750	96	52	1	30	123	98	193	2.60e-19	87.4
MS.gene57075.t1	MTR_3g091620	32.857	140	69	2	46	165	101	235	3.48e-19	87.0
MS.gene57075.t1	MTR_6g087150	48.571	70	36	0	45	114	92	161	4.56e-19	87.0
MS.gene57075.t1	MTR_1g074190	50.000	78	33	1	46	117	99	176	1.30e-18	85.1
MS.gene57075.t1	MTR_1g070140	37.705	122	64	4	42	158	99	213	1.34e-18	85.1
MS.gene57075.t1	MTR_4g120270	48.649	74	36	1	46	119	80	151	2.97e-18	83.2
MS.gene57075.t1	MTR_8g023400	48.750	80	32	2	39	114	85	159	4.29e-18	83.6
MS.gene57075.t1	MTR_7g076250	41.772	79	45	1	41	118	58	136	6.37e-18	80.5
MS.gene57075.t1	MTR_3g099650	43.750	80	42	1	41	117	102	181	6.71e-18	83.6
MS.gene57075.t1	MTR_8g009190	52.113	71	31	1	50	117	80	150	8.54e-18	82.4
MS.gene57075.t1	MTR_5g064980	46.053	76	38	1	45	117	107	182	9.90e-18	83.2
MS.gene57075.t1	MTR_3g078450	38.043	92	55	1	33	122	63	154	1.08e-17	80.5
MS.gene57075.t1	MTR_2g044570	54.545	66	27	1	55	117	63	128	1.53e-17	81.6
MS.gene57075.t1	MTR_3g078470	36.957	92	56	1	33	122	63	154	1.63e-17	80.1
MS.gene57075.t1	MTR_7g113800	44.737	76	37	2	46	117	91	165	2.70e-17	81.6
MS.gene57075.t1	MTR_4g030070	38.776	98	47	2	20	117	71	155	4.83e-17	79.0
MS.gene57075.t1	MTR_1g082550	46.053	76	36	2	46	117	98	172	5.13e-17	80.9
MS.gene57075.t1	MTR_6g055030	39.535	86	49	1	46	128	97	182	6.89e-17	80.1
MS.gene57075.t1	MTR_5g064990	48.000	75	36	1	46	117	114	188	7.08e-17	80.5
MS.gene57075.t1	MTR_1g100737	40.506	79	42	1	44	117	90	168	7.90e-17	77.8
MS.gene57075.t1	MTR_6g007680	45.070	71	39	0	47	117	41	111	8.18e-17	76.6
MS.gene57075.t1	MTR_7g073200	42.045	88	49	2	37	123	72	158	9.59e-17	77.0
MS.gene57075.t1	MTR_1g043410	47.222	72	35	1	46	114	94	165	1.01e-16	79.7
MS.gene57075.t1	MTR_7g116220	46.667	75	37	1	46	117	70	144	1.31e-16	76.6
MS.gene57075.t1	MTR_7g116180	46.667	75	37	1	46	117	67	141	3.97e-16	75.5
MS.gene57075.t1	MTR_3g086730	38.095	126	60	4	7	128	20	131	5.33e-16	76.3
MS.gene57075.t1	MTR_2g090905	41.379	87	45	1	38	118	31	117	5.56e-16	73.6
MS.gene57075.t1	MTR_2g090950	42.045	88	43	2	38	118	83	169	7.20e-16	75.9
MS.gene57075.t1	MTR_1g070425	46.667	75	37	1	46	117	68	142	7.49e-16	74.7
MS.gene57075.t1	MTR_3g094000	40.000	90	50	2	31	117	76	164	8.35e-16	74.3
MS.gene57075.t1	MTR_7g096690	43.038	79	41	1	46	120	70	148	1.26e-15	76.6
MS.gene57075.t1	MTR_4g056590	42.105	76	41	2	46	121	100	172	1.62e-15	73.9
MS.gene57075.t1	MTR_1g492830	41.892	74	42	1	46	118	61	134	2.05e-15	74.3
MS.gene57075.t1	MTR_3g086700	37.302	126	61	4	7	128	20	131	2.15e-15	73.6
MS.gene57075.t1	MTR_7g117540	50.667	75	28	3	47	117	77	146	2.18e-15	75.5
MS.gene57075.t1	MTR_8g027980	52.778	72	31	2	49	117	93	164	3.56e-15	74.3
MS.gene57075.t1	MTR_2g096350	46.053	76	39	1	42	115	103	178	5.90e-15	72.8
MS.gene57075.t1	MTR_7g116200	47.143	70	36	1	46	115	68	136	6.45e-15	71.6
MS.gene57075.t1	MTR_5g024730	38.824	85	42	2	42	120	73	153	6.59e-15	72.8
MS.gene57075.t1	MTR_8g099495	40.816	98	48	2	28	115	293	390	7.30e-15	74.7
MS.gene57075.t1	MTR_3g464780	31.169	154	77	4	43	192	99	227	7.61e-15	73.2
MS.gene57075.t1	MTR_7g079280	40.000	75	39	2	46	116	80	152	9.36e-15	71.6
MS.gene57075.t1	MTR_8g028465	40.845	71	40	1	46	116	90	158	1.33e-14	71.6
MS.gene57075.t1	MTR_3g114500	40.476	84	47	1	44	127	46	126	1.46e-14	69.7
MS.gene57075.t1	MTR_8g032690	57.447	47	20	0	72	118	19	65	1.64e-14	68.9
MS.gene57075.t1	MTR_3g071300	43.590	78	40	1	46	119	76	153	1.86e-14	70.5
MS.gene57075.t1	MTR_8g073120	38.750	80	39	2	42	115	66	141	2.10e-14	71.2
MS.gene57075.t1	MTR_2g436100	43.836	73	41	0	46	118	93	165	2.15e-14	72.4
MS.gene57075.t1	MTR_2g035800	41.558	77	45	0	44	120	74	150	3.13e-14	69.7
MS.gene57075.t1	MTR_5g076990	41.026	78	42	1	46	119	76	153	4.41e-14	71.6
MS.gene57075.t1	MTR_2g055790	35.833	120	58	4	1	120	48	148	5.30e-14	69.7
MS.gene57075.t1	MTR_7g116210	45.714	70	37	1	46	115	10	78	5.84e-14	67.4
MS.gene57075.t1	MTR_3g116000	61.702	47	18	0	71	117	103	149	6.00e-14	69.7
MS.gene57075.t1	MTR_7g079290	35.294	85	51	2	46	126	125	209	8.30e-14	69.7
MS.gene57075.t1	MTR_3g095560	59.574	47	19	0	71	117	135	181	1.58e-13	69.3
MS.gene57075.t1	MTR_4g094725	41.026	78	44	1	44	119	76	153	1.80e-13	67.4
MS.gene57075.t1	MTR_3g081030	44.444	81	44	1	47	127	95	174	3.44e-13	67.8
MS.gene57075.t1	MTR_8g092990	57.447	47	20	0	71	117	140	186	3.78e-13	68.2
MS.gene57075.t1	MTR_8g092970	57.447	47	20	0	71	117	130	176	4.73e-13	67.4
MS.gene57075.t1	MTR_8g092840	38.462	78	47	1	41	117	108	185	6.62e-13	67.4
MS.gene57075.t1	MTR_8g092960	55.319	47	21	0	71	117	129	175	7.45e-13	67.0
MS.gene57075.t1	MTR_3g078120	50.000	62	29	1	55	114	66	127	7.52e-13	65.5
MS.gene57075.t1	MTR_7g108855	41.892	74	41	1	47	118	67	140	8.89e-13	66.2
MS.gene57075.t1	MTR_5g009200	34.821	112	71	1	5	114	281	392	9.59e-13	68.6
MS.gene57075.t1	MTR_5g026730	57.778	45	19	0	72	116	94	138	9.69e-13	65.9
MS.gene57075.t1	MTR_6g055030	49.180	61	30	1	69	128	91	151	1.33e-12	67.4
MS.gene57075.t1	MTR_8g092930	39.726	73	43	1	46	117	112	184	1.58e-12	66.2
MS.gene57075.t1	MTR_5g009210	43.421	76	41	1	41	114	282	357	1.75e-12	67.4
MS.gene57075.t1	MTR_5g009190	42.105	76	42	1	41	114	261	336	2.58e-12	67.0
MS.gene57075.t1	MTR_6g007670	44.615	65	35	1	52	115	151	215	2.58e-12	65.9
MS.gene57075.t1	MTR_6g007670	54.167	48	22	0	70	117	31	78	3.51e-12	65.5
MS.gene57075.t1	MTR_8g071815	56.818	44	19	0	72	115	90	133	2.63e-12	64.7
MS.gene57075.t1	MTR_8g092985	38.667	75	45	1	44	117	109	183	2.78e-12	65.1
MS.gene57075.t1	MTR_3g012550	32.258	93	59	2	46	134	80	172	3.59e-12	64.3
MS.gene57075.t1	MTR_5g092790	40.845	71	40	1	46	114	157	227	4.37e-12	65.1
MS.gene57075.t1	MTR_4g077570	52.000	50	20	2	68	114	7	55	5.47e-12	61.2
MS.gene57075.t1	MTR_5g082940	36.364	77	47	1	46	120	75	151	6.27e-12	63.5
MS.gene57075.t1	MTR_8g066740	38.158	76	43	1	46	117	76	151	7.04e-12	65.5
MS.gene57075.t1	MTR_8g093010	36.000	75	47	1	44	117	105	179	8.82e-12	63.9
MS.gene57075.t1	MTR_8g098770	49.206	63	29	2	53	114	69	129	1.15e-11	62.4
MS.gene57075.t1	MTR_5g026720	37.500	72	40	1	50	116	66	137	1.31e-11	62.4
MS.gene57075.t1	MTR_8g099515	41.860	86	46	2	46	129	309	392	1.59e-11	64.7
MS.gene57075.t1	MTR_5g071930	38.889	72	41	1	46	117	66	134	1.99e-11	62.4
MS.gene57075.t1	MTR_3g091200	35.897	78	48	1	41	116	298	375	2.04e-11	64.3
MS.gene57075.t1	MTR_1g026070	37.975	79	46	1	44	122	46	121	2.08e-11	60.8
MS.gene57075.t1	MTR_8g074900	37.037	81	46	2	35	114	146	222	2.87e-11	62.8
MS.gene57075.t1	MTR_5g092750	39.437	71	41	1	46	114	157	227	2.96e-11	62.8
MS.gene57075.t1	MTR_3g078520	35.526	76	48	1	46	120	76	151	4.18e-11	60.8
MS.gene57075.t1	MTR_1g056350	38.298	94	47	4	28	116	60	147	4.42e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57075.t1	AT5G40250	60.185	324	90	11	6	306	68	375	2.34e-116	340
MS.gene57075.t1	AT1G23980	55.970	268	92	5	9	272	81	326	1.23e-78	244
MS.gene57075.t1	AT4G30400	39.498	319	133	12	13	276	77	390	5.43e-52	177
MS.gene57075.t1	AT2G18650	38.944	303	150	11	6	279	62	358	3.01e-51	174
MS.gene57075.t1	AT3G48030	47.644	191	83	5	35	224	91	265	7.16e-49	164
MS.gene57075.t1	AT3G48030	47.644	191	83	5	35	224	169	343	4.45e-48	164
MS.gene57075.t1	AT5G57750	63.636	88	31	1	28	114	77	164	7.02e-34	123
MS.gene57075.t1	AT5G05810	53.521	71	33	0	44	114	118	188	5.35e-22	95.5
MS.gene57075.t1	AT3G03550	47.619	84	44	0	37	120	124	207	1.09e-21	94.0
MS.gene57075.t1	AT2G20030	53.521	71	33	0	45	115	97	167	2.85e-21	93.2
MS.gene57075.t1	AT5G17600	42.056	107	57	1	9	115	84	185	5.30e-21	92.4
MS.gene57075.t1	AT4G28890	44.554	101	46	2	45	137	96	194	7.09e-21	92.8
MS.gene57075.t1	AT1G72220	46.154	91	47	1	39	129	144	232	8.45e-21	92.4
MS.gene57075.t1	AT4G09110	42.574	101	53	3	37	132	84	184	1.42e-20	90.1
MS.gene57075.t1	AT1G35330	37.097	124	64	2	11	131	76	188	1.74e-20	90.5
MS.gene57075.t1	AT4G33565	48.101	79	41	0	39	117	184	262	5.10e-20	89.7
MS.gene57075.t1	AT3G60220	52.113	71	31	1	50	117	92	162	5.54e-20	89.0
MS.gene57075.t1	AT2G47560	48.837	86	42	1	43	126	77	162	1.08e-19	86.3
MS.gene57075.t1	AT5G10380	46.341	82	44	0	36	117	99	180	2.37e-19	86.7
MS.gene57075.t1	AT4G09130	42.857	91	48	2	46	132	91	181	2.49e-19	87.4
MS.gene57075.t1	AT4G40070	42.000	100	44	4	46	141	95	184	6.08e-19	85.9
MS.gene57075.t1	AT2G35000	40.367	109	60	2	17	120	75	183	6.12e-19	86.7
MS.gene57075.t1	AT3G16720	50.000	74	36	1	46	119	94	166	1.63e-18	84.3
MS.gene57075.t1	AT5G43420	43.678	87	35	1	42	114	94	180	2.40e-18	84.7
MS.gene57075.t1	AT1G53820	46.053	76	40	1	42	117	91	165	2.81e-18	84.0
MS.gene57075.t1	AT1G04360	47.619	84	37	2	41	117	96	179	4.02e-18	84.3
MS.gene57075.t1	AT1G72200	48.000	75	36	1	46	117	115	189	5.36e-18	84.0
MS.gene57075.t1	AT4G09120	46.667	75	37	1	46	117	94	168	1.02e-17	82.8
MS.gene57075.t1	AT2G42350	42.553	94	42	3	46	131	75	164	1.71e-17	80.1
MS.gene57075.t1	AT1G22500	45.455	77	39	1	44	117	87	163	1.88e-17	82.4
MS.gene57075.t1	AT2G34990	44.318	88	42	3	46	127	67	153	2.78e-17	80.9
MS.gene57075.t1	AT2G34990	44.318	88	42	3	46	127	89	175	2.93e-17	81.3
MS.gene57075.t1	AT4G15975	30.534	131	77	3	46	165	51	178	4.88e-17	79.3
MS.gene57075.t1	AT3G18930	45.122	82	35	1	46	117	120	201	1.32e-16	80.1
MS.gene57075.t1	AT3G18930	45.122	82	35	1	46	117	120	201	1.32e-16	80.1
MS.gene57075.t1	AT3G18930	45.122	82	35	1	46	117	120	201	1.32e-16	80.1
MS.gene57075.t1	AT1G20823	46.667	75	39	1	44	117	82	156	4.00e-16	75.9
MS.gene57075.t1	AT2G35420	38.542	96	54	2	24	114	50	145	5.17e-16	76.6
MS.gene57075.t1	AT5G01880	42.466	73	42	0	45	117	78	150	7.33e-16	73.9
MS.gene57075.t1	AT1G72310	42.105	76	43	1	42	117	98	172	7.71e-16	77.0
MS.gene57075.t1	AT3G62690	44.444	72	40	0	47	118	88	159	9.65e-16	75.9
MS.gene57075.t1	AT2G42360	40.476	84	49	1	36	118	71	154	1.33e-15	75.1
MS.gene57075.t1	AT5G46650	39.423	104	58	3	22	121	62	164	1.88e-15	75.5
MS.gene57075.t1	AT3G05200	43.421	76	38	2	46	117	99	173	2.28e-15	76.3
MS.gene57075.t1	AT4G09100	44.737	76	37	2	46	117	56	130	2.48e-15	72.0
MS.gene57075.t1	AT2G27940	38.776	98	54	4	46	140	115	209	3.77e-15	73.9
MS.gene57075.t1	AT4G17905	45.570	79	33	2	45	117	126	200	3.92e-15	75.1
MS.gene57075.t1	AT1G76410	45.333	75	40	1	44	117	75	149	6.57e-15	72.0
MS.gene57075.t1	AT2G46160	39.241	79	40	1	46	116	104	182	6.93e-15	72.8
MS.gene57075.t1	AT2G46160	39.241	79	40	1	46	116	104	182	6.93e-15	72.8
MS.gene57075.t1	AT4G17905	45.570	79	33	2	45	117	152	226	7.19e-15	74.3
MS.gene57075.t1	AT1G33480	44.444	72	39	1	46	117	75	145	9.13e-15	73.2
MS.gene57075.t1	AT4G35480	57.447	47	20	0	71	117	112	158	1.13e-14	72.0
MS.gene57075.t1	AT3G10910	41.096	73	43	0	45	117	87	159	1.25e-14	71.2
MS.gene57075.t1	AT3G61550	36.585	82	41	1	46	116	99	180	1.39e-14	72.0
MS.gene57075.t1	AT4G10160	43.056	72	40	1	46	117	72	142	1.70e-14	72.0
MS.gene57075.t1	AT3G14320	43.478	69	38	1	46	114	63	130	2.87e-14	70.9
MS.gene57075.t1	AT5G27420	43.421	76	38	2	46	117	95	169	3.14e-14	72.8
MS.gene57075.t1	AT5G05280	40.278	72	42	1	46	117	88	158	4.12e-14	69.7
MS.gene57075.t1	AT2G35910	46.377	69	37	0	46	114	121	189	4.89e-14	70.5
MS.gene57075.t1	AT4G10150	43.056	72	40	1	46	117	86	156	7.55e-14	70.1
MS.gene57075.t1	AT5G07040	32.967	91	57	2	46	134	66	154	2.53e-13	67.0
MS.gene57075.t1	AT4G17920	41.558	77	42	1	44	117	79	155	5.48e-13	68.6
MS.gene57075.t1	AT2G17450	51.064	47	23	0	71	117	101	147	7.91e-13	66.2
MS.gene57075.t1	AT2G18670	43.243	74	41	1	46	118	83	156	1.25e-12	65.9
MS.gene57075.t1	AT3G19140	34.831	89	57	1	30	118	38	125	1.93e-12	64.3
MS.gene57075.t1	AT5G53110	42.353	85	43	2	38	116	290	374	2.44e-12	67.4
MS.gene57075.t1	AT1G49220	38.961	77	42	2	44	117	106	180	2.67e-12	65.9
MS.gene57075.t1	AT1G32361	36.905	84	50	2	46	128	130	211	3.23e-12	66.6
MS.gene57075.t1	AT1G49230	53.191	47	22	0	71	117	130	176	5.48e-12	64.7
MS.gene57075.t1	AT3G18773	37.662	77	43	2	44	117	101	175	6.74e-12	64.3
MS.gene57075.t1	AT4G24015	43.478	69	38	1	53	121	87	154	1.12e-11	62.8
MS.gene57075.t1	AT1G49210	37.662	77	43	2	44	117	106	180	1.20e-11	63.9
MS.gene57075.t1	AT2G46493	28.448	116	76	3	4	116	71	182	1.49e-11	62.8
MS.gene57075.t1	AT1G49200	36.364	77	44	2	44	117	107	181	1.55e-11	63.5
MS.gene57075.t1	AT4G11360	37.895	95	46	4	23	114	45	129	3.25e-11	61.2
MS.gene57075.t1	AT5G58580	41.333	75	43	1	44	117	109	183	3.50e-11	63.5
MS.gene57075.t1	AT1G28040	32.353	102	62	3	18	116	200	297	4.53e-11	63.2
MS.gene57075.t1	AT1G71980	41.558	77	42	3	46	120	205	280	5.31e-11	63.5
MS.gene57075.t1	AT1G22670	38.462	78	45	3	46	121	205	281	7.70e-11	62.8
MS.gene57075.t1	AT2G44578	50.000	46	23	0	72	117	70	115	9.37e-11	59.7
MS.gene57075.t1	AT4G30370	38.889	72	44	0	43	114	86	157	1.00e-10	60.1

Find 56 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTGGCCATAACCAAATTT+TGG	0.157592	6.2:+3441666	None:intergenic
TATTCGATTTCGATGATCTT+AGG	0.158395	6.2:-3442074	MS.gene57075:CDS
AATTCTACTCTCATGGTTTA+AGG	0.162913	6.2:+3441804	None:intergenic
GGAAACAAGTAGTAGTAATT+TGG	0.288672	6.2:-3441893	MS.gene57075:CDS
GTTGAAGAAGTTGTTGATAA+AGG	0.314100	6.2:-3441988	MS.gene57075:CDS
TTGAAGAAGTTGTTGATAAA+GGG	0.320710	6.2:-3441987	MS.gene57075:CDS
GTTATCAAAGGAATTGAAAA+TGG	0.356686	6.2:-3441766	MS.gene57075:CDS
AACAAAGATTTGCAGAATCA+TGG	0.368301	6.2:-3441580	MS.gene57075:CDS
AGAGGAACACTTCAAACTAA+TGG	0.369520	6.2:-3442117	MS.gene57075:CDS
CTATTTCCAAAATTTGGTTA+TGG	0.370519	6.2:-3441672	MS.gene57075:CDS
TGTAATGGAGAGAATGGATT+TGG	0.372483	6.2:-3442033	MS.gene57075:CDS
TTGAATTTCCCGAGTCGAAC+TGG	0.376307	6.2:+3441958	None:intergenic
TGGTTGGAAATTCAGAGTTA+AGG	0.382406	6.2:-3441831	MS.gene57075:CDS
GGTTGGAAATTCAGAGTTAA+GGG	0.387873	6.2:-3441830	MS.gene57075:CDS
AGTTCTTTAGAAGCTCATAA+TGG	0.409499	6.2:-3441625	MS.gene57075:CDS
GTAGAGAGAATGAAGAAGTA+GGG	0.416897	6.2:-3441915	MS.gene57075:CDS
ATTCGATTTCGATGATCTTA+GGG	0.431362	6.2:-3442073	MS.gene57075:CDS
ATATAAGTTGTATAGATACT+TGG	0.434910	6.2:-3442170	MS.gene57075:CDS
CGCAAGAAGATGTTATTCAA+TGG	0.442403	6.2:-3441869	MS.gene57075:CDS
AAATCTTTGTTAAGATATGA+TGG	0.460353	6.2:+3441592	None:intergenic
TTTCAATTCAAGGAGATTGT+TGG	0.461922	6.2:-3442285	MS.gene57075:CDS
GGTAGAGAGAATGAAGAAGT+AGG	0.472099	6.2:-3441916	MS.gene57075:CDS
AGATCATCGAAATCGAATAT+CGG	0.475880	6.2:+3442078	None:intergenic
TTAGAAGAACTTGATGAAGA+TGG	0.478882	6.2:+3442426	None:intergenic
AATTTGGTTATGGCCAAGAA+AGG	0.479921	6.2:-3441662	MS.gene57075:CDS
CATGATGAATTCTACTCTCA+TGG	0.489369	6.2:+3441797	None:intergenic
AATGGGATCTTATCAATATG+TGG	0.489833	6.2:-3441851	MS.gene57075:CDS
TCTTTGTGTTGAGAGTGATT+TGG	0.490017	6.2:-3441737	MS.gene57075:CDS
GCAAGAAGATGTTATTCAAT+GGG	0.490217	6.2:-3441868	MS.gene57075:CDS
TGTCCTTGTAATGGAGAGAA+TGG	0.491168	6.2:-3442039	MS.gene57075:CDS
AAGATTAGTAATGTTAGTAA+AGG	0.491990	6.2:-3441706	MS.gene57075:CDS
ATCAAAGGAATTGAAAATGG+TGG	0.506466	6.2:-3441763	MS.gene57075:CDS
AAACAAACAGCACAATCAAA+AGG	0.507813	6.2:+3442252	None:intergenic
ACAAAGATTTGCAGAATCAT+GGG	0.517329	6.2:-3441579	MS.gene57075:CDS
ATTTGGTTATGGCCAAGAAA+GGG	0.522743	6.2:-3441661	MS.gene57075:CDS
TGAAGAAGTTGTTGATAAAG+GGG	0.524827	6.2:-3441986	MS.gene57075:CDS
GGATCTTATCAATATGTGGT+TGG	0.530819	6.2:-3441847	MS.gene57075:CDS
GATGATCTTAGGGAAGATGA+TGG	0.535942	6.2:-3442063	MS.gene57075:CDS
ATTTGCAGAATCATGGGATG+AGG	0.541198	6.2:-3441573	MS.gene57075:CDS
GATGATGGTTGTCCTTGTAA+TGG	0.542547	6.2:-3442048	MS.gene57075:CDS
TCAAAGGAATTGAAAATGGT+GGG	0.548623	6.2:-3441762	MS.gene57075:CDS
TTTAGAAGCTCATAATGGAA+TGG	0.557904	6.2:-3441620	MS.gene57075:CDS
AATCCATTCTCTCCATTACA+AGG	0.564226	6.2:+3442036	None:intergenic
TGAAGTGTTCCTCTACAAAG+AGG	0.570614	6.2:+3442126	None:intergenic
TAGAGAGAATGAAGAAGTAG+GGG	0.574565	6.2:-3441914	MS.gene57075:CDS
GTAGAATTCATCATGATACA+AGG	0.593018	6.2:-3441789	MS.gene57075:CDS
GAAATTCAAGAAGACGAATG+TGG	0.607434	6.2:-3441944	MS.gene57075:CDS
CATGATACAAGGGTTATCAA+AGG	0.608541	6.2:-3441778	MS.gene57075:CDS
TCTACTTGTCCTCTTTGTAG+AGG	0.612228	6.2:-3442135	MS.gene57075:CDS
TACAACTTATATGAAAAGCA+TGG	0.613483	6.2:+3442181	None:intergenic
TTTGCAGAATCATGGGATGA+GGG	0.613784	6.2:-3441572	MS.gene57075:CDS
TGAAAAGCATGGCTACACAT+AGG	0.613953	6.2:+3442192	None:intergenic
AAGAAGACGAATGTGGAAGT+CGG	0.638238	6.2:-3441937	MS.gene57075:CDS
TAGAATTCATCATGATACAA+GGG	0.648957	6.2:-3441788	MS.gene57075:CDS
TGTGTTGAGAGTGATTTGGA+AGG	0.653262	6.2:-3441733	MS.gene57075:CDS
CATGGGATGAGGGAAACAGA+TGG	0.665117	6.2:-3441562	MS.gene57075:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATCTTTGTTAAGATATGA+TGG	+	chr6.2:3442686-3442705	None:intergenic	20.0%
!!	AAGATTAGTAATGTTAGTAA+AGG	-	chr6.2:3442569-3442588	MS.gene57075:intron	20.0%
!!	ATATAAGTTGTATAGATACT+TGG	-	chr6.2:3442105-3442124	MS.gene57075:CDS	20.0%
!!	CATCAAATTTATCAAAAAGA+TGG	-	chr6.2:3441624-3441643	MS.gene57075:CDS	20.0%
!!!	GTTTTTCTATTTCCAAAATT+TGG	-	chr6.2:3442597-3442616	MS.gene57075:intron	20.0%
!	CTATTTCCAAAATTTGGTTA+TGG	-	chr6.2:3442603-3442622	MS.gene57075:intron	25.0%
!	GTATGTTCAAATAACAACTT+AGG	+	chr6.2:3441576-3441595	None:intergenic	25.0%
!	GTTATCAAAGGAATTGAAAA+TGG	-	chr6.2:3442509-3442528	MS.gene57075:intron	25.0%
!	TACAACTTATATGAAAAGCA+TGG	+	chr6.2:3442097-3442116	None:intergenic	25.0%
!	TAGAATTCATCATGATACAA+GGG	-	chr6.2:3442487-3442506	MS.gene57075:intron	25.0%
!!	TTGAAGAAGTTGTTGATAAA+GGG	-	chr6.2:3442288-3442307	MS.gene57075:CDS	25.0%
	AAACAAACAGCACAATCAAA+AGG	+	chr6.2:3442026-3442045	None:intergenic	30.0%
	AACAAAGATTTGCAGAATCA+TGG	-	chr6.2:3442695-3442714	MS.gene57075:intron	30.0%
	AATGGGATCTTATCAATATG+TGG	-	chr6.2:3442424-3442443	MS.gene57075:CDS	30.0%
	AATTCTACTCTCATGGTTTA+AGG	+	chr6.2:3442474-3442493	None:intergenic	30.0%
	ACAAAGATTTGCAGAATCAT+GGG	-	chr6.2:3442696-3442715	MS.gene57075:intron	30.0%
	AGATCATCGAAATCGAATAT+CGG	+	chr6.2:3442200-3442219	None:intergenic	30.0%
	ATCAAAGGAATTGAAAATGG+TGG	-	chr6.2:3442512-3442531	MS.gene57075:intron	30.0%
	ATCTCCTTGAATTGAAAAAC+AGG	+	chr6.2:3441987-3442006	None:intergenic	30.0%
	ATTCGATTTCGATGATCTTA+GGG	-	chr6.2:3442202-3442221	MS.gene57075:CDS	30.0%
	GAACTTGATGAAGATTGTAA+TGG	+	chr6.2:3441687-3441706	None:intergenic	30.0%
	GCAAGAAGATGTTATTCAAT+GGG	-	chr6.2:3442407-3442426	MS.gene57075:CDS	30.0%
	GGAAACAAGTAGTAGTAATT+TGG	-	chr6.2:3442382-3442401	MS.gene57075:CDS	30.0%
	GTAGAATTCATCATGATACA+AGG	-	chr6.2:3442486-3442505	MS.gene57075:intron	30.0%
	TATTCGATTTCGATGATCTT+AGG	-	chr6.2:3442201-3442220	MS.gene57075:CDS	30.0%
	TCAAAGGAATTGAAAATGGT+GGG	-	chr6.2:3442513-3442532	MS.gene57075:intron	30.0%
	TGTAATGGTAATTGTGATGA+TGG	+	chr6.2:3441672-3441691	None:intergenic	30.0%
	TTAGAAGAACTTGATGAAGA+TGG	+	chr6.2:3441852-3441871	None:intergenic	30.0%
	TTTCAATTCAAGGAGATTGT+TGG	-	chr6.2:3441990-3442009	MS.gene57075:CDS	30.0%
!	AGTTCTTTAGAAGCTCATAA+TGG	-	chr6.2:3442650-3442669	MS.gene57075:intron	30.0%
!	GAAGAAAATTTTCCCTTTCT+TGG	+	chr6.2:3442629-3442648	None:intergenic	30.0%
!	GTTGAAGAAGTTGTTGATAA+AGG	-	chr6.2:3442287-3442306	MS.gene57075:CDS	30.0%
!	TGAAGAAGTTGTTGATAAAG+GGG	-	chr6.2:3442289-3442308	MS.gene57075:CDS	30.0%
!	TTTAGAAGCTCATAATGGAA+TGG	-	chr6.2:3442655-3442674	MS.gene57075:intron	30.0%
!!	ACTACCTGTTTTTCAATTCA+AGG	-	chr6.2:3441980-3441999	MS.gene57075:CDS	30.0%
!!	CTTTTTCATCTTCATGATTC+TGG	-	chr6.2:3441933-3441952	MS.gene57075:CDS	30.0%
!!!	GCTGTTTTGTTTTTCATATC+TGG	-	chr6.2:3441789-3441808	MS.gene57075:CDS	30.0%
	AATCCATTCTCTCCATTACA+AGG	+	chr6.2:3442242-3442261	None:intergenic	35.0%
	AATTTGGTTATGGCCAAGAA+AGG	-	chr6.2:3442613-3442632	MS.gene57075:intron	35.0%
	ACTATGATGAAAAGAACAGC+TGG	+	chr6.2:3441762-3441781	None:intergenic	35.0%
	AGAGGAACACTTCAAACTAA+TGG	-	chr6.2:3442158-3442177	MS.gene57075:CDS	35.0%
	ATTTGGTTATGGCCAAGAAA+GGG	-	chr6.2:3442614-3442633	MS.gene57075:intron	35.0%
	CATGATACAAGGGTTATCAA+AGG	-	chr6.2:3442497-3442516	MS.gene57075:intron	35.0%
	CATGATGAATTCTACTCTCA+TGG	+	chr6.2:3442481-3442500	None:intergenic	35.0%
	CGCAAGAAGATGTTATTCAA+TGG	-	chr6.2:3442406-3442425	MS.gene57075:CDS	35.0%
	GAAATTCAAGAAGACGAATG+TGG	-	chr6.2:3442331-3442350	MS.gene57075:CDS	35.0%
	GGATCTTATCAATATGTGGT+TGG	-	chr6.2:3442428-3442447	MS.gene57075:CDS	35.0%
	GGTTGGAAATTCAGAGTTAA+GGG	-	chr6.2:3442445-3442464	MS.gene57075:CDS	35.0%
	GTAGAGAGAATGAAGAAGTA+GGG	-	chr6.2:3442360-3442379	MS.gene57075:CDS	35.0%
	TAGAGAGAATGAAGAAGTAG+GGG	-	chr6.2:3442361-3442380	MS.gene57075:CDS	35.0%
	TCTTTGTGTTGAGAGTGATT+TGG	-	chr6.2:3442538-3442557	MS.gene57075:intron	35.0%
	TGGACTAATCTTAGTACTAG+AGG	+	chr6.2:3441742-3441761	None:intergenic	35.0%
	TGGTTGGAAATTCAGAGTTA+AGG	-	chr6.2:3442444-3442463	MS.gene57075:CDS	35.0%
	TTAGTACTAGAGGATGAAGA+TGG	+	chr6.2:3441732-3441751	None:intergenic	35.0%
!	AGCTGTTCTTTTCATCATAG+TGG	-	chr6.2:3441761-3441780	MS.gene57075:CDS	35.0%
!	TCTTGGCCATAACCAAATTT+TGG	+	chr6.2:3442612-3442631	None:intergenic	35.0%
!	TGTAATGGAGAGAATGGATT+TGG	-	chr6.2:3442242-3442261	MS.gene57075:CDS	35.0%
!!	AATGGTAATTGTGATGATGG+TGG	+	chr6.2:3441669-3441688	None:intergenic	35.0%
	AAGAAGACGAATGTGGAAGT+CGG	-	chr6.2:3442338-3442357	MS.gene57075:CDS	40.0%
	ATTTGCAGAATCATGGGATG+AGG	-	chr6.2:3442702-3442721	MS.gene57075:intron	40.0%
	GATGATCTTAGGGAAGATGA+TGG	-	chr6.2:3442212-3442231	MS.gene57075:CDS	40.0%
	GGTAGAGAGAATGAAGAAGT+AGG	-	chr6.2:3442359-3442378	MS.gene57075:CDS	40.0%
	TCTACTTGTCCTCTTTGTAG+AGG	-	chr6.2:3442140-3442159	MS.gene57075:CDS	40.0%
	TTTGCAGAATCATGGGATGA+GGG	-	chr6.2:3442703-3442722	MS.gene57075:intron	40.0%
!	GATGATGGTTGTCCTTGTAA+TGG	-	chr6.2:3442227-3442246	MS.gene57075:CDS	40.0%
!	TGAAAAGCATGGCTACACAT+AGG	+	chr6.2:3442086-3442105	None:intergenic	40.0%
!	TGAAGTGTTCCTCTACAAAG+AGG	+	chr6.2:3442152-3442171	None:intergenic	40.0%
!	TGTCCTTGTAATGGAGAGAA+TGG	-	chr6.2:3442236-3442255	MS.gene57075:CDS	40.0%
!	TGTGTTGAGAGTGATTTGGA+AGG	-	chr6.2:3442542-3442561	MS.gene57075:intron	40.0%
	GTACTAGAGGATGAAGATGG+TGG	+	chr6.2:3441729-3441748	None:intergenic	45.0%
	TTGAATTTCCCGAGTCGAAC+TGG	+	chr6.2:3442320-3442339	None:intergenic	45.0%
!!	GGTAATTGTGATGATGGTGG+TGG	+	chr6.2:3441666-3441685	None:intergenic	45.0%
	CATGGGATGAGGGAAACAGA+TGG	-	chr6.2:3442713-3442732	MS.gene57075:intron	50.0%
!	GGGGTTTTGCCAGTTCGACT+CGG	-	chr6.2:3442308-3442327	MS.gene57075:CDS	55.0%
!	GGGTTTTGCCAGTTCGACTC+GGG	-	chr6.2:3442309-3442328	MS.gene57075:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	3441555	3442742	3441555	ID=MS.gene57075
chr6.2	mRNA	3441555	3442742	3441555	ID=MS.gene57075.t1;Parent=MS.gene57075
chr6.2	exon	3442729	3442742	3442729	ID=MS.gene57075.t1.exon1;Parent=MS.gene57075.t1
chr6.2	CDS	3442729	3442742	3442729	ID=cds.MS.gene57075.t1;Parent=MS.gene57075.t1
chr6.2	exon	3441555	3442464	3441555	ID=MS.gene57075.t1.exon2;Parent=MS.gene57075.t1
chr6.2	CDS	3441555	3442464	3441555	ID=cds.MS.gene57075.t1;Parent=MS.gene57075.t1

Gene Sequence

>MS.gene57075

ATGTCAGAAACTATGTATCCTAAGTTGTTATTTGAACATACTCATTTAAGAATGTCTTTGATTAATCATCATCAAATTTATCAAAAAGATGGTTACTTTATGAATTTTCCACCACCATCATCACAATTACCATTACAATCTTCATCAAGTTCTTCAACAACATCATCAACACCACCATCTTCATCCTCTAGTACTAAGATTAGTCCAGCTGTTCTTTTCATCATAGTGGTTCTTGCTGTTTTGTTTTTCATATCTGGTTTACTTCATTTGCTTGTTAGATTTCTTATAAAGCATCCATCTTCATCAAGTTCTTCTAATAGACAACAACATCATAATGAAATTTCAACTTCTGATGCACTTCAAAGACAGCTACAACAGCTTTTTCATCTTCATGATTCTGGTTTAGATCAAGCTTTTATTGATGCACTACCTGTTTTTCAATTCAAGGAGATTGTTGGTTTGAATGAGCCTTTTGATTGTGCTGTTTGTTTGTGTGAGTTTACTGAGAAAGATAAGCTGAGATTGTTACCTATGTGTAGCCATGCTTTTCATATAAGTTGTATAGATACTTGGCTACTTTCAAACTCTACTTGTCCTCTTTGTAGAGGAACACTTCAAACTAATGGATTTTCTATTGAAAATCCGATATTCGATTTCGATGATCTTAGGGAAGATGATGGTTGTCCTTGTAATGGAGAGAATGGATTTGGTACTAGACAAAAAGAAATTGTTGTTGAAGAAGTTGTTGATAAAGGGGTTTTGCCAGTTCGACTCGGGAAATTCAAGAAGACGAATGTGGAAGTCGGTAGAGAGAATGAAGAAGTAGGGGAAACAAGTAGTAGTAATTTGGACGCAAGAAGATGTTATTCAATGGGATCTTATCAATATGTGGTTGGAAATTCAGAGTTAAGGGTAGCCTTAAACCATGAGAGTAGAATTCATCATGATACAAGGGTTATCAAAGGAATTGAAAATGGTGGGAATCTTTGTGTTGAGAGTGATTTGGAAGGAAAGAAGATTAGTAATGTTAGTAAAGGTGAAAGTTTTTCTATTTCCAAAATTTGGTTATGGCCAAGAAAGGGAAAATTTTCTTCTAGTTCTTTAGAAGCTCATAATGGAATGGCATTGCCATCATATCTTAACAAAGATTTGCAGAATCATGGGATGAGGGAAACAGATGGTGTATGA

Protein sequence

>MS.gene57075.t1

MSETIFLIKHPSSSSSSNRQQHHNEISTSDALQRQLQQLFHLHDSGLDQAFIDALPVFQFKEIVGLNEPFDCAVCLCEFTEKDKLRLLPMCSHAFHISCIDTWLLSNSTCPLCRGTLQTNGFSIENPIFDFDDLREDDGCPCNGENGFGTRQKEIVVEEVVDKGVLPVRLGKFKKTNVEVGRENEEVGETSSSNLDARRCYSMGSYQYVVGNSELRVALNHESRIHHDTRVIKGIENGGNLCVESDLEGKKISNVSKGESFSISKIWLWPRKGKFSSSSLEAHNGMALPSYLNKDLQNHGMRETDGV