AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58091

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58091.t1 MTR_8g093330 96.124 258 7 1 1 258 67 321 0.0 505
MS.gene58091.t1 MTR_1g061600 52.893 242 109 2 12 250 269 508 5.11e-84 260
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58091.t1 AT3G18680 87.597 258 30 1 3 258 81 338 6.89e-166 462
MS.gene58091.t1 AT3G18680 78.397 287 27 2 7 258 85 371 6.42e-159 446
MS.gene58091.t1 AT3G10030 51.667 240 111 2 11 247 297 534 1.07e-78 247
MS.gene58091.t1 AT3G10030 49.767 215 103 2 11 222 297 509 4.69e-67 216

Find 67 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAAACCCTTCTCCATTT+TGG 0.146176 4.2:+9313244 None:intergenic
GCCAACTCTCTCACCTTTAA+TGG 0.225362 4.2:+9310649 None:intergenic
GAGAGAGTTGGCACTTTGAT+TGG 0.297652 4.2:-9310638 MS.gene58091:CDS
CGCTCATCCGCTGATTATAT+TGG 0.327877 4.2:-9311807 MS.gene58091:CDS
ATGAATCTAGTAAGCCAAAA+TGG 0.334137 4.2:-9313258 MS.gene58091:CDS
AACATAGAGAAGGCCATTAA+AGG 0.337991 4.2:-9310662 MS.gene58091:CDS
TTCCGTGGAGCCTCGTGGGC+TGG 0.355160 4.2:-9311846 MS.gene58091:CDS
TGTCAAGAAAATAATATTCC+TGG 0.362510 4.2:-9310835 MS.gene58091:intron
CGCGCTTGAGGGTTACGCTT+TGG 0.368748 4.2:+9310934 None:intergenic
AGAGAGTTGGCACTTTGATT+GGG 0.369637 4.2:-9310637 MS.gene58091:CDS
CAGAAGGGCTGTGAGGCATT+TGG 0.389622 4.2:-9311302 MS.gene58091:CDS
CTCAAAGCTACAAATGTTGA+TGG 0.395632 4.2:-9310973 MS.gene58091:CDS
TCTTGATACACTAACCTATC+AGG 0.400311 4.2:-9310908 MS.gene58091:CDS
AGGTTGCACTAGTTGTTGGT+GGG 0.406958 4.2:-9311878 MS.gene58091:CDS
ACAGCCCTTCTGCGTATATA+CGG 0.417983 4.2:+9311313 None:intergenic
AATGCAGTTTGTACTCTAGT+TGG 0.432080 4.2:+9311358 None:intergenic
GCTCATCCGCTGATTATATT+GGG 0.442411 4.2:-9311806 MS.gene58091:intron
TGATTTATCGTGTAGGATGC+TGG 0.449284 4.2:-9311434 MS.gene58091:intron
CAGGTTGCACTAGTTGTTGG+TGG 0.453785 4.2:-9311879 MS.gene58091:CDS
TTTCAGGTTGCACTAGTTGT+TGG 0.459501 4.2:-9311882 MS.gene58091:intron
ATGGCCTTCTCTATGTTACC+TGG 0.476740 4.2:+9310668 None:intergenic
GTTAGCGGAGAAGCACTTGC+TGG 0.485759 4.2:-9313217 MS.gene58091:CDS
GGTTGCACTAGTTGTTGGTG+GGG 0.491388 4.2:-9311877 MS.gene58091:CDS
TCATTCATTGACAGTGCAAA+CGG 0.494942 4.2:+9313277 None:intergenic
GACACAAAGTTATGGCAGTA+AGG 0.512526 4.2:+9310854 None:intergenic
GCTGTGAGGCATTTGGAGAA+AGG 0.516252 4.2:-9311295 MS.gene58091:CDS
TATCAAGAAGCCGCGCTTGA+GGG 0.517581 4.2:+9310923 None:intergenic
AGCTCAGAATATTGACCCTA+AGG 0.522546 4.2:-9313188 MS.gene58091:intron
CATCTTCCGTGGAGCCTCGT+GGG 0.524475 4.2:-9311850 MS.gene58091:CDS
TATGTCCATTGCGAGGGAAG+TGG 0.526722 4.2:-9312252 MS.gene58091:CDS
TGAGGCATTTGGAGAAAGGA+AGG 0.528380 4.2:-9311291 MS.gene58091:CDS
CAAGCAACAATGGAGAGTAT+TGG 0.530508 4.2:-9311385 MS.gene58091:CDS
GTTGCACTAGTTGTTGGTGG+GGG 0.534947 4.2:-9311876 MS.gene58091:CDS
GCCTCGTGGGCTGGAAGTAG+TGG 0.535611 4.2:-9311837 MS.gene58091:CDS
CTAGTAAGCCAAAATGGAGA+AGG 0.541861 4.2:-9313252 MS.gene58091:CDS
AACAAAAGATCTGTCAGTGA+TGG 0.552161 4.2:-9310878 MS.gene58091:CDS
AGCTGCCACTTCCCTCGCAA+TGG 0.553085 4.2:+9312247 None:intergenic
AGTCACCTCAATGCCATGGC+GGG 0.556629 4.2:+9312220 None:intergenic
ACATCTTCCGTGGAGCCTCG+TGG 0.568791 4.2:-9311851 MS.gene58091:CDS
TTCCAGCCCACGAGGCTCCA+CGG 0.573341 4.2:+9311844 None:intergenic
TGACAGTGCAAACGGAGACA+TGG 0.577417 4.2:+9313285 None:intergenic
GAGAGTTGGCACTTTGATTG+GGG 0.578321 4.2:-9310636 MS.gene58091:CDS
AACTGCATTTCGCATGTCTG+AGG 0.580501 4.2:-9311344 MS.gene58091:CDS
GCCATTAAAGGTGAGAGAGT+TGG 0.582841 4.2:-9310650 MS.gene58091:CDS
GCAGTGTCTGTGGTAAAGAA+TGG 0.584543 4.2:+9311238 None:intergenic
GTATCAAGAAGCCGCGCTTG+AGG 0.587429 4.2:+9310922 None:intergenic
CAGAACCGTATATACGCAGA+AGG 0.587562 4.2:-9311318 MS.gene58091:CDS
GTGGCAGCTGTAACCCGCCA+TGG 0.592749 4.2:-9312233 MS.gene58091:CDS
TATATTTCTTCAAGCAACAA+TGG 0.604226 4.2:-9311395 MS.gene58091:CDS
AAGTGCTGCGGCAGTGTCTG+TGG 0.607764 4.2:+9311228 None:intergenic
TAGTAAGCCAAAATGGAGAA+GGG 0.608078 4.2:-9313251 MS.gene58091:CDS
CAATATAATCAGCGGATGAG+CGG 0.613358 4.2:+9311808 None:intergenic
GGTAACTATGTCCATTGCGA+GGG 0.613640 4.2:-9312258 MS.gene58091:CDS
CAAACCAGGTAACATAGAGA+AGG 0.618925 4.2:-9310672 MS.gene58091:CDS
TGTAACCCGCCATGGCATTG+AGG 0.619412 4.2:-9312225 MS.gene58091:intron
GTGTAGTCACCTCAATGCCA+TGG 0.622683 4.2:+9312216 None:intergenic
GAGGCATTTGGAGAAAGGAA+GGG 0.627211 4.2:-9311290 MS.gene58091:CDS
TTCTGCACATCTAAGTGCTG+CGG 0.632064 4.2:+9311216 None:intergenic
AGGTAACTATGTCCATTGCG+AGG 0.645668 4.2:-9312259 MS.gene58091:CDS
GCACTTTGATTGGGGCAACG+TGG 0.653598 4.2:-9310628 MS.gene58091:CDS
GTCTTCAATCTAAACAAACC+AGG 0.655408 4.2:-9310686 MS.gene58091:CDS
TTATCACCCAATATAATCAG+CGG 0.667055 4.2:+9311800 None:intergenic
ACCACTACTTCCAGCCCACG+AGG 0.679613 4.2:+9311836 None:intergenic
TAGTCACCTCAATGCCATGG+CGG 0.694102 4.2:+9312219 None:intergenic
AGAACCGTATATACGCAGAA+GGG 0.702130 4.2:-9311317 MS.gene58091:CDS
CAAAGCGTAACCCTCAAGCG+CGG 0.708280 4.2:-9310933 MS.gene58091:CDS
ATATACGCAGAAGGGCTGTG+AGG 0.766075 4.2:-9311309 MS.gene58091:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATATAAATATAAAAC+TGG - chr4.2:9311486-9311505 MS.gene58091:intron 10.0%
!! AAAATAAAGATATAAATTGC+AGG + chr4.2:9310732-9310751 None:intergenic 15.0%
!! AAGTAAATAGCAATAAATAT+CGG + chr4.2:9312864-9312883 None:intergenic 15.0%
!!! AAAATGCTAAATTTTTACTT+TGG - chr4.2:9312354-9312373 MS.gene58091:intron 15.0%
!!! AAATGCTAAATTTTTACTTT+GGG - chr4.2:9312355-9312374 MS.gene58091:intron 15.0%
!!! ACATTGTATTAAACATTTTT+AGG + chr4.2:9312402-9312421 None:intergenic 15.0%
!!! AGACAATTTTTTATTAATAC+AGG + chr4.2:9310939-9310958 None:intergenic 15.0%
!! AAAACTATAAACTAATGAAC+AGG + chr4.2:9312807-9312826 None:intergenic 20.0%
!! AAATACAATTACAAAAAAGC+AGG + chr4.2:9312434-9312453 None:intergenic 20.0%
!! ACTTTGCAATATAATCTTTA+GGG - chr4.2:9311834-9311853 MS.gene58091:CDS 20.0%
!! ATTATAAATAACTGAAAGCT+CGG + chr4.2:9310810-9310829 None:intergenic 20.0%
!! GCTTTCAGTTATTTATAATA+TGG - chr4.2:9310811-9310830 MS.gene58091:intron 20.0%
!! TACTTTGCAATATAATCTTT+AGG - chr4.2:9311833-9311852 MS.gene58091:CDS 20.0%
!!! AAACTGGTAATAAACAATTT+TGG - chr4.2:9311502-9311521 MS.gene58091:intron 20.0%
!!! AACTGGTAATAAACAATTTT+GGG - chr4.2:9311503-9311522 MS.gene58091:intron 20.0%
!!! AAGTTTTGAATGAAGATTAA+AGG + chr4.2:9312141-9312160 None:intergenic 20.0%
!!! AATGCTAAATTTTTACTTTG+GGG - chr4.2:9312356-9312375 MS.gene58091:intron 20.0%
!!! AGATTTTGATTTTGTTTTTG+TGG - chr4.2:9311595-9311614 MS.gene58091:intron 20.0%
!!! GATTTTGATTTTGTTTTTGT+GGG - chr4.2:9311596-9311615 MS.gene58091:intron 20.0%
!!! TGATTACACAGTTAATTTTT+CGG - chr4.2:9310967-9310986 MS.gene58091:CDS 20.0%
! ACTACAAAAAATAAAACCGA+AGG + chr4.2:9311724-9311743 None:intergenic 25.0%
! ATATGGCAAGATATTTCATT+TGG + chr4.2:9311550-9311569 None:intergenic 25.0%
! ATCAGGACAATAAATACAAT+AGG + chr4.2:9313147-9313166 None:intergenic 25.0%
! CTTTGCAATATAATCTTTAG+GGG - chr4.2:9311835-9311854 MS.gene58091:CDS 25.0%
! GAGCAGTATATATACAAATT+TGG - chr4.2:9312198-9312217 MS.gene58091:intron 25.0%
! TAAAAAGTGAGATGAAATCT+GGG + chr4.2:9311446-9311465 None:intergenic 25.0%
! TATATTTCTTCAAGCAACAA+TGG - chr4.2:9312492-9312511 MS.gene58091:intron 25.0%
! TCTTGGTTAAAAGAGATTAA+GGG + chr4.2:9312115-9312134 None:intergenic 25.0%
! TGTCAAGAAAATAATATTCC+TGG - chr4.2:9313052-9313071 MS.gene58091:intron 25.0%
! TTAAAAAGTGAGATGAAATC+TGG + chr4.2:9311447-9311466 None:intergenic 25.0%
! TTCTTGGTTAAAAGAGATTA+AGG + chr4.2:9312116-9312135 None:intergenic 25.0%
!! ACAAACTTTTTACTAAATGC+AGG + chr4.2:9312253-9312272 None:intergenic 25.0%
!! ACAGTTTGGTTTATAATGAT+TGG - chr4.2:9311975-9311994 MS.gene58091:intron 25.0%
!! ATTGAATCATTTCAAGTCAA+AGG + chr4.2:9311938-9311957 None:intergenic 25.0%
!! ATTTTCTTGACACAAAGTTA+TGG + chr4.2:9313044-9313063 None:intergenic 25.0%
!! CAGTTTGGTTTATAATGATT+GGG - chr4.2:9311976-9311995 MS.gene58091:intron 25.0%
!! TTGAATCATTTCAAGTCAAA+GGG + chr4.2:9311937-9311956 None:intergenic 25.0%
!! TTGTATTTGATTTATCGTGT+AGG - chr4.2:9312446-9312465 MS.gene58091:intron 25.0%
!!! AATGATTCAATGCTTTTTAC+TGG - chr4.2:9311947-9311966 MS.gene58091:intron 25.0%
!!! ATGAAGATTAAAGGTATTCT+TGG + chr4.2:9312132-9312151 None:intergenic 25.0%
!!! ATTTTTTCACTAGTTTCCTT+CGG - chr4.2:9311705-9311724 MS.gene58091:intron 25.0%
!!! TACTTTCAGCATTAGTTTTT+TGG - chr4.2:9310913-9310932 MS.gene58091:CDS 25.0%
!!! TTTTGTTATTACATCCTGAT+AGG + chr4.2:9312996-9313015 None:intergenic 25.0%
AAAAAGTGAGATGAAATCTG+GGG + chr4.2:9311445-9311464 None:intergenic 30.0%
ATAAACTCCCCAAATAATAC+TGG + chr4.2:9311897-9311916 None:intergenic 30.0%
ATGAATCTAGTAAGCCAAAA+TGG - chr4.2:9310629-9310648 MS.gene58091:CDS 30.0%
TACACTTAGACAAAAGTCTA+CGG + chr4.2:9312333-9312352 None:intergenic 30.0%
TGATTGAAATCAACTCAGAT+TGG - chr4.2:9313120-9313139 MS.gene58091:intron 30.0%
TGTAGTCACCAGTATTATTT+GGG - chr4.2:9311886-9311905 MS.gene58091:intron 30.0%
TTATCACCCAATATAATCAG+CGG + chr4.2:9312090-9312109 None:intergenic 30.0%
! ATTCTTTTCAGTTAATGCAG+AGG - chr4.2:9312886-9312905 MS.gene58091:intron 30.0%
!! ACTTTGCTCTAGCTTTAAAT+AGG - chr4.2:9311107-9311126 MS.gene58091:intron 30.0%
!! CTTTGCTCTAGCTTTAAATA+GGG - chr4.2:9311108-9311127 MS.gene58091:intron 30.0%
!! TAAACATTTTTAGGCAACAG+AGG + chr4.2:9312393-9312412 None:intergenic 30.0%
!!! TGATTTTGTTTTTGTGGGTT+TGG - chr4.2:9311601-9311620 MS.gene58091:intron 30.0%
!!! TTTTACTGGTTTTCACAGTT+TGG - chr4.2:9311961-9311980 MS.gene58091:intron 30.0%
AAAAGTGAGATGAAATCTGG+GGG + chr4.2:9311444-9311463 None:intergenic 35.0%
AACAAAAGATCTGTCAGTGA+TGG - chr4.2:9313009-9313028 MS.gene58091:intron 35.0%
AACATAGAGAAGGCCATTAA+AGG - chr4.2:9313225-9313244 MS.gene58091:CDS 35.0%
AAGTCAAGAGCAACATCATA+TGG + chr4.2:9311567-9311586 None:intergenic 35.0%
AATGCAGTTTGTACTCTAGT+TGG + chr4.2:9312532-9312551 None:intergenic 35.0%
ACAGATTACAAAACAGGTAC+CGG + chr4.2:9311869-9311888 None:intergenic 35.0%
ACCATGCATTCAATTAACCA+AGG + chr4.2:9311779-9311798 None:intergenic 35.0%
ACGAAGTAGCCAACAAAAAT+TGG + chr4.2:9311082-9311101 None:intergenic 35.0%
CATGCATTCAATTAACCAAG+GGG + chr4.2:9311777-9311796 None:intergenic 35.0%
CATTCTCGCATCTTCATAAA+GGG - chr4.2:9312759-9312778 MS.gene58091:intron 35.0%
CCATGCATTCAATTAACCAA+GGG + chr4.2:9311778-9311797 None:intergenic 35.0%
CTCAAAGCTACAAATGTTGA+TGG - chr4.2:9312914-9312933 MS.gene58091:intron 35.0%
CTGTAGTCACCAGTATTATT+TGG - chr4.2:9311885-9311904 MS.gene58091:intron 35.0%
GTAGTCACCAGTATTATTTG+GGG - chr4.2:9311887-9311906 MS.gene58091:intron 35.0%
GTCTTCAATCTAAACAAACC+AGG - chr4.2:9313201-9313220 MS.gene58091:CDS 35.0%
GTGACTACAGATTACAAAAC+AGG + chr4.2:9311875-9311894 None:intergenic 35.0%
TACTAAATGCAGGAAATTGC+AGG + chr4.2:9312243-9312262 None:intergenic 35.0%
TAGTAAGCCAAAATGGAGAA+GGG - chr4.2:9310636-9310655 MS.gene58091:CDS 35.0%
TCATTCATTGACAGTGCAAA+CGG + chr4.2:9310613-9310632 None:intergenic 35.0%
TCATTCTCGCATCTTCATAA+AGG - chr4.2:9312758-9312777 MS.gene58091:intron 35.0%
TCTTGATACACTAACCTATC+AGG - chr4.2:9312979-9312998 MS.gene58091:intron 35.0%
TTTAGGCAACAGAGGATATT+TGG + chr4.2:9312385-9312404 None:intergenic 35.0%
! TTGAATGCATGGTTTGAAGT+AGG - chr4.2:9311786-9311805 MS.gene58091:intron 35.0%
!! ATAGAAGTACCAACAGAAGT+TGG - chr4.2:9311269-9311288 MS.gene58091:CDS 35.0%
AAGGACAGATACAGTTCATC+AGG + chr4.2:9313164-9313183 None:intergenic 40.0%
ACCAATCCTACAGTCTACTT+AGG + chr4.2:9311135-9311154 None:intergenic 40.0%
ACTAGTGCAACCTGAAACAT+CGG + chr4.2:9312002-9312021 None:intergenic 40.0%
AGAACCGTATATACGCAGAA+GGG - chr4.2:9312570-9312589 MS.gene58091:intron 40.0%
AGAGAGTTGGCACTTTGATT+GGG - chr4.2:9313250-9313269 MS.gene58091:CDS 40.0%
AGCCAACAAAAATTGGCATC+AGG + chr4.2:9311075-9311094 None:intergenic 40.0%
AGCTCAGAATATTGACCCTA+AGG - chr4.2:9310699-9310718 MS.gene58091:intron 40.0%
AGTTGACACGTAAACCACAA+GGG - chr4.2:9311306-9311325 MS.gene58091:CDS 40.0%
CAAACCAGGTAACATAGAGA+AGG - chr4.2:9313215-9313234 MS.gene58091:CDS 40.0%
CAACAAAAATTGGCATCAGG+AGG + chr4.2:9311072-9311091 None:intergenic 40.0%
CAATATAATCAGCGGATGAG+CGG + chr4.2:9312082-9312101 None:intergenic 40.0%
CCAATCCTACAGTCTACTTA+GGG + chr4.2:9311134-9311153 None:intergenic 40.0%
CCCTTGGTTAATTGAATGCA+TGG - chr4.2:9311775-9311794 MS.gene58091:intron 40.0%
CTAGTAAGCCAAAATGGAGA+AGG - chr4.2:9310635-9310654 MS.gene58091:CDS 40.0%
GACACAAAGTTATGGCAGTA+AGG + chr4.2:9313036-9313055 None:intergenic 40.0%
GTTTGTCTGCTTTACTTACC+AGG + chr4.2:9313073-9313092 None:intergenic 40.0%
TCAATACAGTCTCCAAGTCT+CGG + chr4.2:9311411-9311430 None:intergenic 40.0%
TGAAGACTACAACTGTTCCA+AGG + chr4.2:9313188-9313207 None:intergenic 40.0%
TGATTTATCGTGTAGGATGC+TGG - chr4.2:9312453-9312472 MS.gene58091:intron 40.0%
TTACAAATCACCCCACAACA+AGG + chr4.2:9311369-9311388 None:intergenic 40.0%
! AGCAAAACCCTTCTCCATTT+TGG + chr4.2:9310646-9310665 None:intergenic 40.0%
! AGGGTTTTGCTCAAAGTTAG+CGG - chr4.2:9310655-9310674 MS.gene58091:CDS 40.0%
! CAAGCAACAATGGAGAGTAT+TGG - chr4.2:9312502-9312521 MS.gene58091:intron 40.0%
! CATGGTTTGAAGTAGGTTTG+GGG - chr4.2:9311793-9311812 MS.gene58091:intron 40.0%
! CTCCTGATGCCAATTTTTGT+TGG - chr4.2:9311070-9311089 MS.gene58091:intron 40.0%
! GCATGGTTTGAAGTAGGTTT+GGG - chr4.2:9311792-9311811 MS.gene58091:intron 40.0%
! GCTCATCCGCTGATTATATT+GGG - chr4.2:9312081-9312100 MS.gene58091:intron 40.0%
! GGGGTGATTTGTAAATACGT+AGG - chr4.2:9311375-9311394 MS.gene58091:CDS 40.0%
! TGCATGGTTTGAAGTAGGTT+TGG - chr4.2:9311791-9311810 MS.gene58091:intron 40.0%
! TGGAGACTGTATTGACCTTT+GGG - chr4.2:9311416-9311435 MS.gene58091:CDS 40.0%
! TTGGAGACTGTATTGACCTT+TGG - chr4.2:9311415-9311434 MS.gene58091:CDS 40.0%
!! AGTTAATTTTTCGGCCCACA+TGG - chr4.2:9310976-9310995 MS.gene58091:CDS 40.0%
!! GTTAATTTTTCGGCCCACAT+GGG - chr4.2:9310977-9310996 MS.gene58091:CDS 40.0%
!! TTTCAGGTTGCACTAGTTGT+TGG - chr4.2:9312005-9312024 MS.gene58091:intron 40.0%
AAAACACGGTATCCGACCTA+GGG + chr4.2:9311176-9311195 None:intergenic 45.0%
AACACCAGACTAACCCATGT+GGG + chr4.2:9310993-9311012 None:intergenic 45.0%
AACTGCATTTCGCATGTCTG+AGG - chr4.2:9312543-9312562 MS.gene58091:intron 45.0%
AACTGTATCTGTCCTTGCCT+TGG - chr4.2:9313168-9313187 MS.gene58091:intron 45.0%
AATGATTGGGCCGATGTTTC+AGG - chr4.2:9311989-9312008 MS.gene58091:intron 45.0%
AATTGCAGGTAGACGACCTT+AGG + chr4.2:9310718-9310737 None:intergenic 45.0%
ACAGCCCTTCTGCGTATATA+CGG + chr4.2:9312577-9312596 None:intergenic 45.0%
ACTACAACTGTTCCAAGGCA+AGG + chr4.2:9313183-9313202 None:intergenic 45.0%
AGGTAACTATGTCCATTGCG+AGG - chr4.2:9311628-9311647 MS.gene58091:intron 45.0%
AGGTTGCACTAGTTGTTGGT+GGG - chr4.2:9312009-9312028 MS.gene58091:intron 45.0%
AGTTGAATTTCGCACCCTTG+TGG + chr4.2:9311323-9311342 None:intergenic 45.0%
ATGGCCTTCTCTATGTTACC+TGG + chr4.2:9313222-9313241 None:intergenic 45.0%
ATTGCAGGTAGACGACCTTA+GGG + chr4.2:9310717-9310736 None:intergenic 45.0%
CAAAACGTTGGGTGTGTATG+TGG - chr4.2:9312282-9312301 MS.gene58091:intron 45.0%
CAGAACCGTATATACGCAGA+AGG - chr4.2:9312569-9312588 MS.gene58091:intron 45.0%
CCAACTGAACCAACTTCTGT+TGG + chr4.2:9311281-9311300 None:intergenic 45.0%
CCCTAAGTAGACTGTAGGAT+TGG - chr4.2:9311131-9311150 MS.gene58091:intron 45.0%
CTTGAACTTGAGACCTTGAG+AGG + chr4.2:9311042-9311061 None:intergenic 45.0%
GAGAGAGTTGGCACTTTGAT+TGG - chr4.2:9313249-9313268 MS.gene58091:CDS 45.0%
GAGAGTTGGCACTTTGATTG+GGG - chr4.2:9313251-9313270 MS.gene58091:CDS 45.0%
GAGTTGACACGTAAACCACA+AGG - chr4.2:9311305-9311324 MS.gene58091:CDS 45.0%
GCAGTGTCTGTGGTAAAGAA+TGG + chr4.2:9312652-9312671 None:intergenic 45.0%
GCCAACTCTCTCACCTTTAA+TGG + chr4.2:9313241-9313260 None:intergenic 45.0%
GCCATTAAAGGTGAGAGAGT+TGG - chr4.2:9313237-9313256 MS.gene58091:CDS 45.0%
GGTAACTATGTCCATTGCGA+GGG - chr4.2:9311629-9311648 MS.gene58091:intron 45.0%
GTCACGAGAGACAAAACGTT+GGG - chr4.2:9312271-9312290 MS.gene58091:intron 45.0%
TAAAACACGGTATCCGACCT+AGG + chr4.2:9311177-9311196 None:intergenic 45.0%
TGTCACGAGAGACAAAACGT+TGG - chr4.2:9312270-9312289 MS.gene58091:intron 45.0%
! AAGGGTGCGAAATTCAACTC+CGG - chr4.2:9311324-9311343 MS.gene58091:CDS 45.0%
! AGAAGTTGGTTCAGTTGGTC+AGG - chr4.2:9311283-9311302 MS.gene58091:CDS 45.0%
! CCAACAGAAGTTGGTTCAGT+TGG - chr4.2:9311278-9311297 MS.gene58091:CDS 45.0%
! CGCTCATCCGCTGATTATAT+TGG - chr4.2:9312080-9312099 MS.gene58091:intron 45.0%
! GAGGCATTTGGAGAAAGGAA+GGG - chr4.2:9312597-9312616 MS.gene58091:intron 45.0%
! GTTTTTGTGGGTTTGGATGC+AGG - chr4.2:9311608-9311627 MS.gene58091:intron 45.0%
! TCAAGTTGTTTGAGACCCCT+TGG - chr4.2:9311759-9311778 MS.gene58091:intron 45.0%
! TGAGGCATTTGGAGAAAGGA+AGG - chr4.2:9312596-9312615 MS.gene58091:intron 45.0%
! TTCTGCACATCTAAGTGCTG+CGG + chr4.2:9312674-9312693 None:intergenic 45.0%
!! TAGTCTGGTGTTGTAGCCTT+GGG - chr4.2:9311001-9311020 MS.gene58091:intron 45.0%
!! TTAGTCTGGTGTTGTAGCCT+TGG - chr4.2:9311000-9311019 MS.gene58091:intron 45.0%
!!! AGGGTTGTCATTTTTGCTGC+TGG - chr4.2:9312616-9312635 MS.gene58091:intron 45.0%
!!! TTTTTTATATTTTTAAAACA+CGG + chr4.2:9311190-9311209 None:intergenic 5.0%
ATATACGCAGAAGGGCTGTG+AGG - chr4.2:9312578-9312597 MS.gene58091:intron 50.0%
CAACACCAGACTAACCCATG+TGG + chr4.2:9310994-9311013 None:intergenic 50.0%
CAGGTTGCACTAGTTGTTGG+TGG - chr4.2:9312008-9312027 MS.gene58091:intron 50.0%
CGAAATTCAACTCCGGCTGT+TGG - chr4.2:9311331-9311350 MS.gene58091:CDS 50.0%
CTTGGATTGTGCTCCTCTCA+AGG - chr4.2:9311026-9311045 MS.gene58091:intron 50.0%
GGTTGCACTAGTTGTTGGTG+GGG - chr4.2:9312010-9312029 MS.gene58091:intron 50.0%
GTGTAGTCACCTCAATGCCA+TGG + chr4.2:9311674-9311693 None:intergenic 50.0%
TAGGGCCCTAAGTAGACTGT+AGG - chr4.2:9311126-9311145 MS.gene58091:intron 50.0%
TAGTCACCTCAATGCCATGG+CGG + chr4.2:9311671-9311690 None:intergenic 50.0%
TATCAAGAAGCCGCGCTTGA+GGG + chr4.2:9312967-9312986 None:intergenic 50.0%
TATGTCCATTGCGAGGGAAG+TGG - chr4.2:9311635-9311654 MS.gene58091:intron 50.0%
TGAAATCTGGGGGATCCCAA+AGG + chr4.2:9311434-9311453 None:intergenic 50.0%
! AGGCATGAGGACCTTGTTGT+GGG - chr4.2:9311355-9311374 MS.gene58091:CDS 50.0%
! ATCTTTAGGGGAAGCTCTGC+CGG - chr4.2:9311847-9311866 MS.gene58091:CDS 50.0%
! CTTGAGAGGAGCACAATCCA+AGG + chr4.2:9311028-9311047 None:intergenic 50.0%
! GCTGTGAGGCATTTGGAGAA+AGG - chr4.2:9312592-9312611 MS.gene58091:intron 50.0%
! GTTGCACTAGTTGTTGGTGG+GGG - chr4.2:9312011-9312030 MS.gene58091:intron 50.0%
!! ATTGGTCCTCCCGGATTAGT+CGG - chr4.2:9311149-9311168 MS.gene58091:intron 50.0%
!!! GTCATTTTTGCTGCTGGCAC+TGG - chr4.2:9312622-9312641 MS.gene58091:intron 50.0%
ACCTAGGGACCGACTAATCC+GGG + chr4.2:9311161-9311180 None:intergenic 55.0%
AGTCACCTCAATGCCATGGC+GGG + chr4.2:9311670-9311689 None:intergenic 55.0%
ATTCAACTCCGGCTGTTGGC+AGG - chr4.2:9311335-9311354 MS.gene58091:CDS 55.0%
CAAAGCGTAACCCTCAAGCG+CGG - chr4.2:9312954-9312973 MS.gene58091:intron 55.0%
CAGGCATGAGGACCTTGTTG+TGG - chr4.2:9311354-9311373 MS.gene58091:CDS 55.0%
GACCTAGGGACCGACTAATC+CGG + chr4.2:9311162-9311181 None:intergenic 55.0%
GAGCACAATCCAAGGTCCCA+AGG + chr4.2:9311020-9311039 None:intergenic 55.0%
GCACTTTGATTGGGGCAACG+TGG - chr4.2:9313259-9313278 MS.gene58091:CDS 55.0%
GGTTAGTGCTCTCCGAGACT+TGG - chr4.2:9311396-9311415 MS.gene58091:CDS 55.0%
GTATCAAGAAGCCGCGCTTG+AGG + chr4.2:9312968-9312987 None:intergenic 55.0%
TGTAACCCGCCATGGCATTG+AGG - chr4.2:9311662-9311681 MS.gene58091:intron 55.0%
! CAGAAGGGCTGTGAGGCATT+TGG - chr4.2:9312585-9312604 MS.gene58091:intron 55.0%
! GGCATGAGGACCTTGTTGTG+GGG - chr4.2:9311356-9311375 MS.gene58091:CDS 55.0%
! GTGTTGTAGCCTTGGGACCT+TGG - chr4.2:9311008-9311027 MS.gene58091:intron 55.0%
!! GACTGTAGGATTGGTCCTCC+CGG - chr4.2:9311140-9311159 MS.gene58091:intron 55.0%
!! GGATTAGTCGGTCCCTAGGT+CGG - chr4.2:9311161-9311180 MS.gene58091:intron 55.0%
!! GTTAGCGGAGAAGCACTTGC+TGG - chr4.2:9310670-9310689 MS.gene58091:CDS 55.0%
ACATCTTCCGTGGAGCCTCG+TGG - chr4.2:9312036-9312055 MS.gene58091:intron 60.0%
ACCACTACTTCCAGCCCACG+AGG + chr4.2:9312054-9312073 None:intergenic 60.0%
CATCTTCCGTGGAGCCTCGT+GGG - chr4.2:9312037-9312056 MS.gene58091:intron 60.0%
CGCGCTTGAGGGTTACGCTT+TGG + chr4.2:9312956-9312975 None:intergenic 60.0%
GGTGGGGGTAACATCTTCCG+TGG - chr4.2:9312026-9312045 MS.gene58091:intron 60.0%
TAGGGACCGACTAATCCGGG+AGG + chr4.2:9311158-9311177 None:intergenic 60.0%
TCCTCATGCCTGCCAACAGC+CGG + chr4.2:9311346-9311365 None:intergenic 60.0%
! AAGTGCTGCGGCAGTGTCTG+TGG + chr4.2:9312662-9312681 None:intergenic 60.0%
! AGCTGCCACTTCCCTCGCAA+TGG + chr4.2:9311643-9311662 None:intergenic 60.0%
!! TCCCGGATTAGTCGGTCCCT+AGG - chr4.2:9311157-9311176 MS.gene58091:intron 60.0%
!! TCGGCCCACATGGGTTAGTC+TGG - chr4.2:9310986-9311005 MS.gene58091:CDS 60.0%
GTGGCAGCTGTAACCCGCCA+TGG - chr4.2:9311654-9311673 MS.gene58091:intron 65.0%
TCCGGCTGTTGGCAGGCATG+AGG - chr4.2:9311342-9311361 MS.gene58091:CDS 65.0%
TTCCAGCCCACGAGGCTCCA+CGG + chr4.2:9312046-9312065 None:intergenic 65.0%
!! GCCTCGTGGGCTGGAAGTAG+TGG - chr4.2:9312050-9312069 MS.gene58091:intron 65.0%
TTCCGTGGAGCCTCGTGGGC+TGG - chr4.2:9312041-9312060 MS.gene58091:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 9310604 9313305 9310604 ID=MS.gene58091
chr4.2 mRNA 9310604 9313305 9310604 ID=MS.gene58091.t1;Parent=MS.gene58091
chr4.2 exon 9313189 9313305 9313189 ID=MS.gene58091.t1.exon1;Parent=MS.gene58091.t1
chr4.2 CDS 9313189 9313305 9313189 ID=cds.MS.gene58091.t1;Parent=MS.gene58091.t1
chr4.2 exon 9312226 9312279 9312226 ID=MS.gene58091.t1.exon2;Parent=MS.gene58091.t1
chr4.2 CDS 9312226 9312279 9312226 ID=cds.MS.gene58091.t1;Parent=MS.gene58091.t1
chr4.2 exon 9311807 9311898 9311807 ID=MS.gene58091.t1.exon3;Parent=MS.gene58091.t1
chr4.2 CDS 9311807 9311898 9311807 ID=cds.MS.gene58091.t1;Parent=MS.gene58091.t1
chr4.2 exon 9311215 9311441 9311215 ID=MS.gene58091.t1.exon4;Parent=MS.gene58091.t1
chr4.2 CDS 9311215 9311441 9311215 ID=cds.MS.gene58091.t1;Parent=MS.gene58091.t1
chr4.2 exon 9310836 9311012 9310836 ID=MS.gene58091.t1.exon5;Parent=MS.gene58091.t1
chr4.2 CDS 9310836 9311012 9310836 ID=cds.MS.gene58091.t1;Parent=MS.gene58091.t1
chr4.2 exon 9310604 9310713 9310604 ID=MS.gene58091.t1.exon6;Parent=MS.gene58091.t1
chr4.2 CDS 9310604 9310713 9310604 ID=cds.MS.gene58091.t1;Parent=MS.gene58091.t1
Gene Sequence

>MS.gene58091

ATGTCTCCGTTTGCACTGTCAATGAATGAATCTAGTAAGCCAAAATGGAGAAGGGTTTTGCTCAAAGTTAGCGGAGAAGCACTTGCTGGAGATCAAGCTCAGAATATTGACCCTAAGGTCGTCTACCTGCAATTTATATCTTTATTTTGTGTTTCAATCTGCTTAACTAAATTAATATTATGTGTGTCAGTCAGTCAAATATTCCGAGCTTTCAGTTATTTATAATATGGTCTTTCATTAAATATTATGAGTGTCACTGTGTCAGTCAAATACTGAAACAATGTTTTCTTCAATTACAATACTACGTACTACTTTCAGCATTAGTTTTTTGGCCTGTATTAATAAAAAATTGTCTAGTATTCATGATTACACAGTTAATTTTTCGGCCCACATGGGTTAGTCTGGTGTTGTAGCCTTGGGACCTTGGATTGTGCTCCTCTCAAGGTCTCAAGTTCAAGTTTGAATCCTCCTGATGCCAATTTTTGTTGGCTACTTCGTACAGAACTTTGCTCTAGCTTTAAATAGGGCCCTAAGTAGACTGTAGGATTGGTCCTCCCGGATTAGTCGGTCCCTAGGTCGGATACCGTGTTTTAAAAATATAAAAAAAATATACTTGATATTTGTTTAATTGTTGTTGATTATAAACTAATATAGATAAGATAGATATAGAAGTACCAACAGAAGTTGGTTCAGTTGGTCAGGAGTTGACACGTAAACCACAAGGGTGCGAAATTCAACTCCGGCTGTTGGCAGGCATGAGGACCTTGTTGTGGGGTGATTTGTAAATACGTAGGTTAGTGCTCTCCGAGACTTGGAGACTGTATTGACCTTTGGGATCCCCCAGATTTCATCTCACTTTTTAATTTTTATCATACAAAAAGAAGAAAATATAAATATAAAACTGGTAATAAACAATTTTGGGAGACATAATCGATGAATGAAACCAAATGAAATATCTTGCCATATGATGTTGCTCTTGACTTTGTTTCTGAGATTTTGATTTTGTTTTTGTGGGTTTGGATGCAGGTAACTATGTCCATTGCGAGGGAAGTGGCAGCTGTAACCCGCCATGGCATTGAGGTGACTACACTATAGTGTATTATTTTTTCACTAGTTTCCTTCGGTTTTATTTTTTGTAGTTTGTCTGCTGCATTTTCAAGTTGTTTGAGACCCCTTGGTTAATTGAATGCATGGTTTGAAGTAGGTTTGGGGAATTATTATTGTTAATGTACTTTGCAATATAATCTTTAGGGGAAGCTCTGCCGGTACCTGTTTTGTAATCTGTAGTCACCAGTATTATTTGGGGAGTTTATTATCTTTGCATGTTAGACCCTTTGACTTGAAATGATTCAATGCTTTTTACTGGTTTTCACAGTTTGGTTTATAATGATTGGGCCGATGTTTCAGGTTGCACTAGTTGTTGGTGGGGGTAACATCTTCCGTGGAGCCTCGTGGGCTGGAAGTAGTGGTCTTGACCGCTCATCCGCTGATTATATTGGGTGATAATTCCCTTAATCTCTTTTAACCAAGAATACCTTTAATCTTCATTCAAAACTTTTTGCATGTATGCAGAAGTCTGTCTTAATTTTATCTAGAGCAGTATATATACAAATTTGGATTCTCTACTTTTGAGTTTCCTGCAATTTCCTGCATTTAGTAAAAAGTTTGTCACGAGAGACAAAACGTTGGGTGTGTATGTGGCTTTATAGCTGTAAAGACAAAAGTACCGTAGACTTTTGTCTAAGTGTAGAAAATGCTAAATTTTTACTTTGGGGAATCCAAATATCCTCTGTTGCCTAAAAATGTTTAATACAATGTAAATTGTGTCCTGCTTTTTTGTAATTGTATTTGATTTATCGTGTAGGATGCTGGCAACAGTCATGAATGCTATATTTCTTCAAGCAACAATGGAGAGTATTGGCATTCCAACTAGAGTACAAACTGCATTTCGCATGTCTGAGGTTGCAGAACCGTATATACGCAGAAGGGCTGTGAGGCATTTGGAGAAAGGAAGGGTTGTCATTTTTGCTGCTGGCACTGGAAATCCATTCTTTACCACAGACACTGCCGCAGCACTTAGATGTGCAGAAAGTAAGTTAACTTTTCGTTGCTGCAATTTTATCTGTTATAGTTGATCGTTAGTTCATATTGCTTTCATTCTCGCATCTTCATAAAGGGTGTATATTTCTAGATTGCAAATCCTGTTCATTAGTTTATAGTTTTGTGCTAAATTTATGAATTAATCATGTGCTTCAAACCGATATTTATTGCTATTTACTTCTATTCTTTTCAGTTAATGCAGAGGTTGTACTCAAAGCTACAAATGTTGATGGAGTATTTGATGATGATCCAAAGCGTAACCCTCAAGCGCGGCTTCTTGATACACTAACCTATCAGGATGTAATAACAAAAGATCTGTCAGTGATGGACCTTACTGCCATAACTTTGTGTCAAGAAAATAATATTCCTGGTAAGTAAAGCAGACAAACATTAAAATAAGTTTTTATTATTTATTTTGATTGAAATCAACTCAGATTGGTCCTATTGTATTTATTGTCCTGATGAACTGTATCTGTCCTTGCCTTGGAACAGTTGTAGTCTTCAATCTAAACAAACCAGGTAACATAGAGAAGGCCATTAAAGGTGAGAGAGTTGGCACTTTGATTGGGGCAACGTGGAATTCTACCATATCAAGAACGTGA
Protein sequence

>MS.gene58091.t1

MSPFALSMNESSKPKWRRVLLKVSGEALAGDQAQNIDPKVTMSIAREVAAVTRHGIEVALVVGGGNIFRGASWAGSSGLDRSSADYIGMLATVMNAIFLQATMESIGIPTRVQTAFRMSEVAEPYIRRRAVRHLEKGRVVIFAAGTGNPFFTTDTAAALRCAEINAEVVLKATNVDGVFDDDPKRNPQARLLDTLTYQDVITKDLSVMDLTAITLCQENNIPVVVFNLNKPGNIEKAIKGERVGTLIGATWNSTISRT