AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCTTCTGGCTTGCTTAA+TGG 0.177034 2.3:-50546105 MS.gene050400:CDS
ATCTTGTTTGATCTTGATTT+TGG 0.229038 2.3:+50547717 None:intergenic
CTACTCATGGCTTTATCTTC+TGG 0.251698 2.3:-50546117 MS.gene050400:CDS
TTAATCATTCTCTTCTTTGA+TGG 0.270861 2.3:+50547293 None:intergenic
ATCAAACAAGATCTTATTCT+TGG 0.275784 2.3:-50547706 MS.gene050400:CDS
TTTCAAACAACAGCACTGTT+AGG 0.320261 2.3:-50547166 MS.gene050400:CDS
AACTTCTTTCCCTTCACATA+TGG 0.359398 2.3:+50546306 None:intergenic
CATCTTGAGGGAAGACTTGA+TGG 0.388275 2.3:+50546157 None:intergenic
ACCATTTGCAGCTGCTGCAA+GGG 0.392222 2.3:+50546353 None:intergenic
GCCCTTGCAGCAGCTGCAAA+TGG 0.406328 2.3:-50546354 MS.gene050400:CDS
CTCTGGAGAAGTGGACCAAC+TGG 0.407869 2.3:-50547110 MS.gene050400:CDS
TTGTACCTTCTAGTGAATCA+TGG 0.424632 2.3:-50547412 MS.gene050400:CDS
CACCATTTGCAGCTGCTGCA+AGG 0.426560 2.3:+50546352 None:intergenic
AGTCTTCCCTCAAGATGTTA+AGG 0.430063 2.3:-50546151 MS.gene050400:CDS
TGATCCCATGATTCACTAGA+AGG 0.441692 2.3:+50547407 None:intergenic
CAAAGAAGAGAATGATTAAG+AGG 0.444414 2.3:-50547289 MS.gene050400:CDS
TCAAACAAGATCTTATTCTT+GGG 0.457205 2.3:-50547705 MS.gene050400:CDS
TTCTCAGTTCCTACTAGATC+AGG 0.466474 2.3:+50547206 None:intergenic
GAGAATGATTAAGAGGATGA+TGG 0.470043 2.3:-50547282 MS.gene050400:CDS
TTCAAACAACAGCACTGTTA+GGG 0.471012 2.3:-50547165 MS.gene050400:CDS
TAGAGAAGAAGATGAAGAAG+TGG 0.474019 2.3:+50547760 None:intergenic
GGATCATCAAGAAGAGGAAA+AGG 0.474295 2.3:-50547390 MS.gene050400:CDS
GAAAGAAGTTGCAAATCAAA+AGG 0.475396 2.3:-50546293 MS.gene050400:CDS
CAAGTATCACCTGATCTAGT+AGG 0.482391 2.3:-50547215 MS.gene050400:CDS
GAGGATGATGGAAGATCAAA+GGG 0.487515 2.3:-50547270 MS.gene050400:CDS
TTGAAGATTTGATAACAAGA+TGG 0.491046 2.3:-50546197 MS.gene050400:CDS
TGTACCTTCTAGTGAATCAT+GGG 0.494972 2.3:-50547411 MS.gene050400:CDS
TTGGTCCACTTCTCCAGAGA+GGG 0.496789 2.3:+50547114 None:intergenic
CTACACCATCACTTAGTATG+TGG 0.505698 2.3:+50547675 None:intergenic
ACACAACTAAGACCCCTCTC+TGG 0.508622 2.3:-50547127 MS.gene050400:CDS
AGTTGCAAATCAAAAGGAAA+AGG 0.513567 2.3:-50546287 MS.gene050400:CDS
AATGTGCACCACAATTCAAA+AGG 0.514128 2.3:-50546251 MS.gene050400:CDS
CAGCCTCCTTAACATCTTGA+GGG 0.514868 2.3:+50546145 None:intergenic
AACAAGATGGAGCAGCAACT+TGG 0.518537 2.3:-50546184 MS.gene050400:CDS
GTTGATTAGTATTGAAGAGA+TGG 0.528693 2.3:+50547786 None:intergenic
TGCACCACAATTCAAAAGGA+AGG 0.532928 2.3:-50546247 MS.gene050400:CDS
GGAGGCTGCTATTCTACTCA+TGG 0.534642 2.3:-50546130 MS.gene050400:CDS
AGAGGATGATGGAAGATCAA+AGG 0.536944 2.3:-50547271 MS.gene050400:CDS
GCAGCCTCCTTAACATCTTG+AGG 0.537105 2.3:+50546144 None:intergenic
AGGATAGAACTTCAATGAAG+TGG 0.543169 2.3:-50547319 MS.gene050400:CDS
AAGACCCCTCTCTGGAGAAG+TGG 0.553472 2.3:-50547119 MS.gene050400:CDS
GGCAGAGAAACCATATGTGA+AGG 0.565007 2.3:-50546316 MS.gene050400:CDS
AAATCTTAGCATTCATCAGA+AGG 0.570288 2.3:+50546078 None:intergenic
CATACTAAGTGATGGTGTAG+AGG 0.591541 2.3:-50547672 MS.gene050400:intron
AAGTGGACCAACTGGTCCCA+AGG 0.591915 2.3:-50547102 MS.gene050400:intron
AGAGAGGGGTCTTAGTTGTG+TGG 0.598153 2.3:+50547129 None:intergenic
ATATGTACCTTGGGACCAGT+TGG 0.609719 2.3:+50547095 None:intergenic
TGGTCCACTTCTCCAGAGAG+GGG 0.626088 2.3:+50547115 None:intergenic
ATCATGGGATCATCAAGAAG+AGG 0.628177 2.3:-50547396 MS.gene050400:CDS
GCAGAGAAACCATATGTGAA+GGG 0.631828 2.3:-50546315 MS.gene050400:CDS
TGCAAATGGTGAGACTCTTG+TGG 0.634220 2.3:-50546340 MS.gene050400:CDS
GCACCACAATTCAAAAGGAA+GGG 0.637198 2.3:-50546246 MS.gene050400:CDS
TCAAACCACATACTAAGTGA+TGG 0.647670 2.3:-50547680 MS.gene050400:CDS
CTTCCCTCAAGATGTTAAGG+AGG 0.655628 2.3:-50546148 MS.gene050400:CDS
CAGTCACTTTGCAATGCCTG+CGG 0.658493 2.3:-50546402 MS.gene050400:CDS
GTTGGTCCACTTCTCCAGAG+AGG 0.682429 2.3:+50547113 None:intergenic
ATCTTGAGGGAAGACTTGAT+GGG 0.684739 2.3:+50546158 None:intergenic
AAATGGTGAGACTCTTGTGG+TGG 0.684853 2.3:-50546337 MS.gene050400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATAATAATGAAAATTTT+AGG - chr2.3:50546533-50546552 MS.gene050400:intron 10.0%
!!! AGATAATAATGAAAATTTTA+GGG - chr2.3:50546534-50546553 MS.gene050400:intron 10.0%
!! CAATAGTAATAAAAAAGAAT+TGG + chr2.3:50546953-50546972 None:intergenic 15.0%
!! TAAACAAAGGTTATTAATTA+TGG - chr2.3:50547181-50547200 MS.gene050400:CDS 15.0%
!!! TTACTATTGAGAAAATTTTA+TGG - chr2.3:50546964-50546983 MS.gene050400:intron 15.0%
!! ACTTTAATGATTGTAAACAA+AGG - chr2.3:50547168-50547187 MS.gene050400:CDS 20.0%
!! ATATCAGATAACAATTCAAA+TGG - chr2.3:50546991-50547010 MS.gene050400:intron 20.0%
!! TACAAAATTCTTAACCTAAA+AGG + chr2.3:50546452-50546471 None:intergenic 20.0%
!! TGTAATTATTTGCAACATAA+TGG + chr2.3:50547218-50547237 None:intergenic 20.0%
!!! AAGTTTGAGGTTAATTAATT+TGG - chr2.3:50546913-50546932 MS.gene050400:intron 20.0%
!!! GTTAATTAATTTGGTATAAC+AGG - chr2.3:50546922-50546941 MS.gene050400:intron 20.0%
! ATCAAACAAGATCTTATTCT+TGG - chr2.3:50546173-50546192 MS.gene050400:CDS 25.0%
! TCAAACAAGATCTTATTCTT+GGG - chr2.3:50546174-50546193 MS.gene050400:CDS 25.0%
! TTAATCATTCTCTTCTTTGA+TGG + chr2.3:50546589-50546608 None:intergenic 25.0%
!! TTCATGATTAAGCTCTTTTA+TGG - chr2.3:50547331-50547350 MS.gene050400:CDS 25.0%
!! TTGAAGATTTGATAACAAGA+TGG - chr2.3:50547682-50547701 MS.gene050400:CDS 25.0%
!!! ATCTTGTTTGATCTTGATTT+TGG + chr2.3:50546165-50546184 None:intergenic 25.0%
!!! TAATGAAAATTTTAGGGATG+AGG - chr2.3:50546540-50546559 MS.gene050400:intron 25.0%
!!! TGTTTTAAAGATGAAGTTTG+AGG - chr2.3:50546900-50546919 MS.gene050400:intron 25.0%
AGGAAAAGGATGAAAAAGAT+TGG - chr2.3:50546503-50546522 MS.gene050400:intron 30.0%
AGTTGCAAATCAAAAGGAAA+AGG - chr2.3:50547592-50547611 MS.gene050400:intron 30.0%
ATATTAGATGTCAGTCTATG+AGG - chr2.3:50547024-50547043 MS.gene050400:intron 30.0%
CAAAGAAGAGAATGATTAAG+AGG - chr2.3:50546590-50546609 MS.gene050400:intron 30.0%
GAAAACTAAAGCCTCTATTA+TGG + chr2.3:50547071-50547090 None:intergenic 30.0%
GAAAGAAGTTGCAAATCAAA+AGG - chr2.3:50547586-50547605 MS.gene050400:intron 30.0%
GAACAAATCTTATACAAGCA+AGG + chr2.3:50547314-50547333 None:intergenic 30.0%
TTGTTTCAGAACCATAATAG+AGG - chr2.3:50547057-50547076 MS.gene050400:intron 30.0%
! ATTAAGCTCTTTTATGGCTT+AGG - chr2.3:50547337-50547356 MS.gene050400:CDS 30.0%
! GTTGATTAGTATTGAAGAGA+TGG + chr2.3:50546096-50546115 None:intergenic 30.0%
AACTTCTTTCCCTTCACATA+TGG + chr2.3:50547576-50547595 None:intergenic 35.0%
AATGTGCACCACAATTCAAA+AGG - chr2.3:50547628-50547647 MS.gene050400:intron 35.0%
AGGATAGAACTTCAATGAAG+TGG - chr2.3:50546560-50546579 MS.gene050400:intron 35.0%
GAGAATGATTAAGAGGATGA+TGG - chr2.3:50546597-50546616 MS.gene050400:intron 35.0%
GTTAATGCGAAGTTATATCG+AGG + chr2.3:50547370-50547389 None:intergenic 35.0%
TAGAGAAGAAGATGAAGAAG+TGG + chr2.3:50546122-50546141 None:intergenic 35.0%
TCAAACCACATACTAAGTGA+TGG - chr2.3:50546199-50546218 MS.gene050400:CDS 35.0%
TGTACCTTCTAGTGAATCAT+GGG - chr2.3:50546468-50546487 MS.gene050400:intron 35.0%
TTCAAACAACAGCACTGTTA+GGG - chr2.3:50546714-50546733 MS.gene050400:intron 35.0%
TTGTACCTTCTAGTGAATCA+TGG - chr2.3:50546467-50546486 MS.gene050400:intron 35.0%
TTTCAAACAACAGCACTGTT+AGG - chr2.3:50546713-50546732 MS.gene050400:intron 35.0%
! CATGTGATTGTCATCCTTTT+AGG - chr2.3:50546435-50546454 MS.gene050400:intron 35.0%
! TTACCCTTCCTTTTGAATTG+TGG + chr2.3:50547639-50547658 None:intergenic 35.0%
!! TTATCTTCTGGCTTGCTTAA+TGG - chr2.3:50547774-50547793 MS.gene050400:CDS 35.0%
!! TTATGGTTCTGAAACAATGC+AGG + chr2.3:50547054-50547073 None:intergenic 35.0%
AGTCTTCCCTCAAGATGTTA+AGG - chr2.3:50547728-50547747 MS.gene050400:CDS 40.0%
ATCATGGGATCATCAAGAAG+AGG - chr2.3:50546483-50546502 MS.gene050400:intron 40.0%
ATCTTGAGGGAAGACTTGAT+GGG + chr2.3:50547724-50547743 None:intergenic 40.0%
CAAGTATCACCTGATCTAGT+AGG - chr2.3:50546664-50546683 MS.gene050400:intron 40.0%
CTACACCATCACTTAGTATG+TGG + chr2.3:50546207-50546226 None:intergenic 40.0%
GCACCACAATTCAAAAGGAA+GGG - chr2.3:50547633-50547652 MS.gene050400:intron 40.0%
GCAGAGAAACCATATGTGAA+GGG - chr2.3:50547564-50547583 MS.gene050400:intron 40.0%
GGATCATCAAGAAGAGGAAA+AGG - chr2.3:50546489-50546508 MS.gene050400:intron 40.0%
TGATCCCATGATTCACTAGA+AGG + chr2.3:50546475-50546494 None:intergenic 40.0%
TGCACCACAATTCAAAAGGA+AGG - chr2.3:50547632-50547651 MS.gene050400:intron 40.0%
TTCTCAGTTCCTACTAGATC+AGG + chr2.3:50546676-50546695 None:intergenic 40.0%
! CATACTAAGTGATGGTGTAG+AGG - chr2.3:50546207-50546226 MS.gene050400:CDS 40.0%
!! AGAGGATGATGGAAGATCAA+AGG - chr2.3:50546608-50546627 MS.gene050400:intron 40.0%
!! CTACTCATGGCTTTATCTTC+TGG - chr2.3:50547762-50547781 MS.gene050400:CDS 40.0%
!! GAGGATGATGGAAGATCAAA+GGG - chr2.3:50546609-50546628 MS.gene050400:intron 40.0%
AAATGGTGAGACTCTTGTGG+TGG - chr2.3:50547542-50547561 MS.gene050400:intron 45.0%
AACAAGATGGAGCAGCAACT+TGG - chr2.3:50547695-50547714 MS.gene050400:CDS 45.0%
ATATGTACCTTGGGACCAGT+TGG + chr2.3:50546787-50546806 None:intergenic 45.0%
CAGCCTCCTTAACATCTTGA+GGG + chr2.3:50547737-50547756 None:intergenic 45.0%
CATCTTGAGGGAAGACTTGA+TGG + chr2.3:50547725-50547744 None:intergenic 45.0%
CCCAAGGTACATATTGCTCA+CGG - chr2.3:50546793-50546812 MS.gene050400:intron 45.0%
CCGTGAGCAATATGTACCTT+GGG + chr2.3:50546796-50546815 None:intergenic 45.0%
CTTCCCTCAAGATGTTAAGG+AGG - chr2.3:50547731-50547750 MS.gene050400:CDS 45.0%
GGCAGAGAAACCATATGTGA+AGG - chr2.3:50547563-50547582 MS.gene050400:intron 45.0%
TCCGTGAGCAATATGTACCT+TGG + chr2.3:50546797-50546816 None:intergenic 45.0%
TGCAAATGGTGAGACTCTTG+TGG - chr2.3:50547539-50547558 MS.gene050400:intron 45.0%
! TTTTCTTTGTCGAATGCCGC+AGG + chr2.3:50547496-50547515 None:intergenic 45.0%
!!! AGATATATATAAAATTTTAA+AGG + chr2.3:50546281-50546300 None:intergenic 5.0%
!!! ATATATATAAAATTTTAAAG+GGG + chr2.3:50546279-50546298 None:intergenic 5.0%
!!! GAAAAAAATATTATTTTAAA+AGG + chr2.3:50546825-50546844 None:intergenic 5.0%
!!! GATATATATAAAATTTTAAA+GGG + chr2.3:50546280-50546299 None:intergenic 5.0%
ACACAACTAAGACCCCTCTC+TGG - chr2.3:50546752-50546771 MS.gene050400:intron 50.0%
ACCATTTGCAGCTGCTGCAA+GGG + chr2.3:50547529-50547548 None:intergenic 50.0%
AGAGAGGGGTCTTAGTTGTG+TGG + chr2.3:50546753-50546772 None:intergenic 50.0%
GCAGCCTCCTTAACATCTTG+AGG + chr2.3:50547738-50547757 None:intergenic 50.0%
GGAGGCTGCTATTCTACTCA+TGG - chr2.3:50547749-50547768 MS.gene050400:CDS 50.0%
TTGGTCCACTTCTCCAGAGA+GGG + chr2.3:50546768-50546787 None:intergenic 50.0%
! CAGTCACTTTGCAATGCCTG+CGG - chr2.3:50547477-50547496 MS.gene050400:intron 50.0%
AAGTGGACCAACTGGTCCCA+AGG - chr2.3:50546777-50546796 MS.gene050400:intron 55.0%
CACCATTTGCAGCTGCTGCA+AGG + chr2.3:50547530-50547549 None:intergenic 55.0%
GTTGGTCCACTTCTCCAGAG+AGG + chr2.3:50546769-50546788 None:intergenic 55.0%
TGGTCCACTTCTCCAGAGAG+GGG + chr2.3:50546767-50546786 None:intergenic 55.0%
! AAGACCCCTCTCTGGAGAAG+TGG - chr2.3:50546760-50546779 MS.gene050400:intron 55.0%
! CTCTGGAGAAGTGGACCAAC+TGG - chr2.3:50546769-50546788 MS.gene050400:intron 55.0%
GCCCTTGCAGCAGCTGCAAA+TGG - chr2.3:50547525-50547544 MS.gene050400:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 50546083 50547818 50546083 ID=MS.gene050400
chr2.3 mRNA 50546083 50547818 50546083 ID=MS.gene050400.t1;Parent=MS.gene050400
chr2.3 exon 50547673 50547818 50547673 ID=MS.gene050400.t1.exon1;Parent=MS.gene050400.t1
chr2.3 CDS 50547673 50547818 50547673 ID=cds.MS.gene050400.t1;Parent=MS.gene050400.t1
chr2.3 exon 50547103 50547444 50547103 ID=MS.gene050400.t1.exon2;Parent=MS.gene050400.t1
chr2.3 CDS 50547103 50547444 50547103 ID=cds.MS.gene050400.t1;Parent=MS.gene050400.t1
chr2.3 exon 50546083 50546421 50546083 ID=MS.gene050400.t1.exon3;Parent=MS.gene050400.t1
chr2.3 CDS 50546083 50546421 50546083 ID=cds.MS.gene050400.t1;Parent=MS.gene050400.t1
Gene Sequence

>MS.gene050400

ATCACAGTCACCATCTCTTCAATACTAATCAACAAGCCACTTCTTCATCTTCTTCTCTATCATACTCTATACTCTTCAACCAAAATCAAGATCAAACAAGATCTTATTCTTGGGAGTCAAACCACATACTAAGTGATGGTGTAGAGGTAACATATACATATAATCATATTATATTATTTAGTATTAAGTAGCACCCCTTTAAAATTTTATATATATCTTTGCTTTAATCAATTTGATCATGCATAAGCTTATTTATATATTTTTTGATTTATTTATTTCTGTTAAAAAAAAAGCTTATTGTTTTAATATGTAGAAATTTATCTTGCTGTTTCAAAAAAAAAAATTGATCAAGCATGTGATTGTCATCCTTTTAGGTTAAGAATTTTGTACCTTCTAGTGAATCATGGGATCATCAAGAAGAGGAAAAGGATGAAAAAGATTGGAAGAAAGAAGATAATAATGAAAATTTTAGGGATGAGGATAGAACTTCAATGAAGTGGATGCCATCAAAGAAGAGAATGATTAAGAGGATGATGGAAGATCAAAGGGCTTCTGAGCAAGAGTTTGAGAAACAAATTAAGCAAGTATCACCTGATCTAGTAGGAACTGAGAATAGTAGCAACAACAATTTTTCAAACAACAGCACTGTTAGGGTTTGTACTGACTGCCACACAACTAAGACCCCTCTCTGGAGAAGTGGACCAACTGGTCCCAAGGTACATATTGCTCACGGATTCTTCCTTTTAAAATAATATTTTTTTCATGATAAATAATTTACTTGTTACATTGTGCTTCTAATATGAACATTTTAGAGAAATGTTTTAAAGATGAAGTTTGAGGTTAATTAATTTGGTATAACAGGTTAATCCAATTCTTTTTTATTACTATTGAGAAAATTTTATGGTGTTATATCAGATAACAATTCAAATGGAAACTGTTGTATATTAGATGTCAGTCTATGAGGTCTACCTGCATTGTTTCAGAACCATAATAGAGGCTTTAGTTTTCATATGTTGATCTCGTGACTATTTTAAATATATTAACGACAAAAACAAATAATTAATTATTTCAGTGTAATTTGAAGCACTTTAATGATTGTAAACAAAGGTTATTAATTATGGTAATTGAAATTCCATTATGTTGCAAATAATTACATCAAATACTACTGATATATACAACTTCTACGACAAACTCTTATATCGCGACATCGATATATAGATTAGATAAACCTTGCTTGTATAAGATTTGTTCATGATTAAGCTCTTTTATGGCTTAGGAACTCATCCTCGATATAACTTCGCATTAACAATGCGAGTATAATTATTTGAAAAAATGAATTATGTGATTTAAAATTTTAAACTAAATATTGAAAAAATACAAATATGTTTTTGTAACAGTCACTTTGCAATGCCTGCGGCATTCGACAAAGAAAAGCGAGACGCGCCCTTGCAGCAGCTGCAAATGGTGAGACTCTTGTGGTGGCAGAGAAACCATATGTGAAGGGAAAGAAGTTGCAAATCAAAAGGAAAAGGTCAAAAACTGATCAATGTGCACCACAATTCAAAAGGAAGGGTAAAAGTGAAAACAAGTGTAATAATTTTGAAGATTTGATAACAAGATGGAGCAGCAACTTGGCTTCCCATCAAGTCTTCCCTCAAGATGTTAAGGAGGCTGCTATTCTACTCATGGCTTTATCTTCTGGCTTGCTTAATGGATTTCCTTCTGATGAATGCTAA

Protein sequence

>MS.gene050400.t1

ITVTISSILINKPLLHLLLYHTLYSSTKIKIKQDLILGSQTTYUVMVURLRILYLLVNHGIIKKRKRMKKIGRKKIIMKILGMRIELQUSGCHQRREULRGUWKIKGLLSKSLRNKLSKYHLIUUELRIVATTIFQTTALLGFVLTATQLRPLSGEVDQLVPSHFAMPAAFDKEKRDAPLQQLQMVRLLWWQRNHMURERSCKSKGKGQKLINVHHNSKGRVKVKTSVIILKIUUQDGAATWLPIKSSLKMLRRLLFYSWLYLLACLMDFLLMNA