Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58342.t1 | QCE02779.1 | 64.2 | 888 | 249 | 8 | 1 | 838 | 1 | 869 | 0.00E+00 | 1101.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58342.t1 | Q9M2E2 | 59.0 | 295 | 113 | 5 | 547 | 838 | 7 | 296 | 9.1e-92 | 339.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58342.t1 | A0A4D6MRN2 | 64.2 | 888 | 249 | 8 | 1 | 838 | 1 | 869 | 0.0e+00 | 1101.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057102 | MS.gene58342 | 0.831578 | 1.54E-55 | -1.69E-46 |
| MS.gene05952 | MS.gene58342 | 0.80555 | 1.26E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene58342 | -0.801445 | 8.97E-49 | -1.69E-46 |
| MS.gene060338 | MS.gene58342 | 0.835524 | 1.60E-56 | -1.69E-46 |
| MS.gene061188 | MS.gene58342 | 0.801161 | 1.03E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58342.t1 | MTR_8g031650 | 57.244 | 566 | 223 | 8 | 277 | 838 | 7 | 557 | 0.0 | 625 |
| MS.gene58342.t1 | MTR_8g031650 | 56.206 | 564 | 228 | 7 | 1 | 560 | 1 | 549 | 0.0 | 612 |
| MS.gene58342.t1 | MTR_8g032010 | 96.233 | 292 | 11 | 0 | 547 | 838 | 7 | 298 | 0.0 | 583 |
| MS.gene58342.t1 | MTR_8g032010 | 84.429 | 289 | 43 | 2 | 1 | 288 | 1 | 288 | 1.39e-171 | 498 |
| MS.gene58342.t1 | MTR_8g032010 | 83.916 | 286 | 44 | 2 | 274 | 558 | 4 | 288 | 1.77e-167 | 487 |
| MS.gene58342.t1 | MTR_8g031990 | 85.959 | 292 | 41 | 0 | 547 | 838 | 7 | 298 | 0.0 | 528 |
| MS.gene58342.t1 | MTR_8g031990 | 84.375 | 288 | 43 | 2 | 1 | 287 | 1 | 287 | 6.99e-170 | 493 |
| MS.gene58342.t1 | MTR_8g031990 | 84.397 | 282 | 42 | 2 | 277 | 557 | 7 | 287 | 4.95e-166 | 483 |
| MS.gene58342.t1 | MTR_8g032020 | 87.762 | 286 | 34 | 1 | 274 | 558 | 5 | 290 | 1.24e-177 | 513 |
| MS.gene58342.t1 | MTR_8g032020 | 83.108 | 296 | 49 | 1 | 544 | 838 | 5 | 300 | 4.97e-175 | 506 |
| MS.gene58342.t1 | MTR_8g032020 | 81.185 | 287 | 53 | 1 | 3 | 288 | 4 | 290 | 2.77e-163 | 476 |
| MS.gene58342.t1 | MTR_8g032000 | 82.534 | 292 | 51 | 0 | 547 | 838 | 7 | 298 | 5.32e-175 | 506 |
| MS.gene58342.t1 | MTR_8g032000 | 84.452 | 283 | 42 | 2 | 277 | 558 | 7 | 288 | 2.67e-166 | 484 |
| MS.gene58342.t1 | MTR_8g032000 | 80.623 | 289 | 54 | 2 | 1 | 288 | 1 | 288 | 1.01e-161 | 472 |
| MS.gene58342.t1 | MTR_8g031650 | 58.065 | 403 | 153 | 7 | 440 | 838 | 1 | 391 | 1.12e-154 | 458 |
| MS.gene58342.t1 | MTR_8g031650 | 55.584 | 394 | 159 | 6 | 171 | 560 | 2 | 383 | 7.00e-144 | 430 |
| MS.gene58342.t1 | MTR_8g031650 | 56.786 | 280 | 116 | 3 | 10 | 287 | 104 | 380 | 3.95e-99 | 314 |
| MS.gene58342.t1 | MTR_8g031980 | 71.575 | 292 | 83 | 0 | 547 | 838 | 7 | 298 | 2.71e-147 | 435 |
| MS.gene58342.t1 | MTR_8g031980 | 69.858 | 282 | 83 | 2 | 277 | 557 | 7 | 287 | 5.32e-136 | 406 |
| MS.gene58342.t1 | MTR_8g031980 | 68.750 | 288 | 88 | 2 | 1 | 287 | 1 | 287 | 1.35e-134 | 402 |
| MS.gene58342.t1 | MTR_8g031960 | 71.972 | 289 | 81 | 0 | 547 | 835 | 8 | 296 | 2.78e-145 | 430 |
| MS.gene58342.t1 | MTR_8g031960 | 70.567 | 282 | 81 | 2 | 277 | 557 | 8 | 288 | 9.28e-137 | 408 |
| MS.gene58342.t1 | MTR_8g031960 | 66.316 | 285 | 94 | 2 | 4 | 287 | 5 | 288 | 1.10e-130 | 392 |
| MS.gene58342.t1 | MTR_8g031940 | 67.918 | 293 | 93 | 1 | 547 | 838 | 7 | 299 | 6.92e-137 | 408 |
| MS.gene58342.t1 | MTR_8g031940 | 65.385 | 286 | 96 | 3 | 277 | 560 | 7 | 291 | 4.96e-125 | 378 |
| MS.gene58342.t1 | MTR_8g031940 | 65.517 | 290 | 97 | 3 | 1 | 288 | 1 | 289 | 6.41e-125 | 377 |
| MS.gene58342.t1 | MTR_7g076660 | 58.362 | 293 | 110 | 3 | 546 | 838 | 6 | 286 | 7.71e-118 | 358 |
| MS.gene58342.t1 | MTR_7g076660 | 56.949 | 295 | 113 | 3 | 1 | 295 | 1 | 281 | 8.53e-112 | 343 |
| MS.gene58342.t1 | MTR_7g076660 | 57.857 | 280 | 105 | 2 | 272 | 551 | 2 | 268 | 2.70e-106 | 328 |
| MS.gene58342.t1 | MTR_7g076655 | 58.020 | 293 | 111 | 3 | 546 | 838 | 6 | 286 | 4.45e-117 | 357 |
| MS.gene58342.t1 | MTR_7g076655 | 57.439 | 289 | 109 | 3 | 1 | 288 | 1 | 276 | 1.19e-109 | 337 |
| MS.gene58342.t1 | MTR_7g076655 | 56.552 | 290 | 112 | 3 | 272 | 560 | 2 | 278 | 8.34e-105 | 325 |
| MS.gene58342.t1 | MTR_7g076660 | 58.219 | 292 | 110 | 3 | 547 | 838 | 8 | 287 | 8.03e-117 | 356 |
| MS.gene58342.t1 | MTR_7g076660 | 56.897 | 290 | 111 | 3 | 6 | 295 | 7 | 282 | 1.69e-110 | 340 |
| MS.gene58342.t1 | MTR_7g076660 | 58.182 | 275 | 102 | 2 | 277 | 551 | 8 | 269 | 2.63e-105 | 326 |
| MS.gene58342.t1 | MTR_8g031900 | 56.164 | 292 | 123 | 2 | 547 | 838 | 7 | 293 | 1.42e-112 | 345 |
| MS.gene58342.t1 | MTR_8g031900 | 54.804 | 281 | 121 | 2 | 1 | 281 | 1 | 275 | 7.87e-104 | 322 |
| MS.gene58342.t1 | MTR_8g031900 | 55.273 | 275 | 117 | 3 | 277 | 551 | 7 | 275 | 1.04e-99 | 311 |
| MS.gene58342.t1 | MTR_8g032030 | 97.126 | 174 | 5 | 0 | 1 | 174 | 1 | 174 | 2.85e-112 | 342 |
| MS.gene58342.t1 | MTR_8g032030 | 82.143 | 168 | 30 | 0 | 277 | 444 | 7 | 174 | 3.64e-89 | 281 |
| MS.gene58342.t1 | MTR_8g032030 | 83.333 | 168 | 27 | 1 | 547 | 713 | 7 | 174 | 6.55e-89 | 281 |
| MS.gene58342.t1 | MTR_8g031630 | 58.844 | 294 | 116 | 4 | 547 | 838 | 7 | 297 | 2.23e-110 | 340 |
| MS.gene58342.t1 | MTR_8g031630 | 57.093 | 289 | 119 | 3 | 1 | 287 | 1 | 286 | 3.50e-105 | 326 |
| MS.gene58342.t1 | MTR_8g031630 | 55.944 | 286 | 121 | 3 | 277 | 560 | 7 | 289 | 3.43e-100 | 313 |
| MS.gene58342.t1 | MTR_8g031910 | 56.997 | 293 | 113 | 3 | 547 | 838 | 7 | 287 | 2.88e-110 | 339 |
| MS.gene58342.t1 | MTR_8g031910 | 59.107 | 291 | 104 | 4 | 6 | 295 | 6 | 282 | 2.04e-109 | 337 |
| MS.gene58342.t1 | MTR_8g031910 | 56.207 | 290 | 112 | 4 | 277 | 565 | 7 | 282 | 1.10e-101 | 317 |
| MS.gene58342.t1 | MTR_8g031600 | 59.044 | 293 | 116 | 2 | 548 | 838 | 8 | 298 | 2.98e-110 | 339 |
| MS.gene58342.t1 | MTR_8g031600 | 56.897 | 290 | 119 | 3 | 1 | 287 | 1 | 287 | 6.19e-101 | 315 |
| MS.gene58342.t1 | MTR_8g031600 | 57.343 | 286 | 116 | 3 | 278 | 560 | 8 | 290 | 1.28e-99 | 311 |
| MS.gene58342.t1 | MTR_8g031610 | 58.219 | 292 | 118 | 3 | 548 | 838 | 6 | 294 | 2.48e-109 | 337 |
| MS.gene58342.t1 | MTR_8g031610 | 55.872 | 281 | 120 | 2 | 8 | 287 | 6 | 283 | 1.70e-100 | 314 |
| MS.gene58342.t1 | MTR_8g031610 | 55.282 | 284 | 123 | 2 | 278 | 560 | 6 | 286 | 1.19e-99 | 311 |
| MS.gene58342.t1 | MTR_7g076665 | 57.432 | 296 | 117 | 5 | 546 | 838 | 6 | 295 | 1.98e-108 | 335 |
| MS.gene58342.t1 | MTR_7g076665 | 57.801 | 282 | 111 | 4 | 7 | 287 | 7 | 281 | 1.61e-103 | 322 |
| MS.gene58342.t1 | MTR_7g076665 | 56.537 | 283 | 115 | 4 | 276 | 557 | 6 | 281 | 7.21e-99 | 310 |
| MS.gene58342.t1 | MTR_7g076660 | 56.777 | 273 | 106 | 3 | 566 | 838 | 2 | 262 | 1.90e-105 | 325 |
| MS.gene58342.t1 | MTR_7g076660 | 55.556 | 270 | 106 | 3 | 26 | 295 | 2 | 257 | 2.83e-99 | 309 |
| MS.gene58342.t1 | MTR_7g076660 | 57.031 | 256 | 97 | 2 | 296 | 551 | 2 | 244 | 8.58e-95 | 298 |
| MS.gene58342.t1 | MTR_5g062280 | 52.397 | 292 | 136 | 2 | 547 | 838 | 7 | 295 | 8.75e-98 | 306 |
| MS.gene58342.t1 | MTR_5g062280 | 51.736 | 288 | 136 | 2 | 1 | 288 | 1 | 285 | 1.75e-92 | 293 |
| MS.gene58342.t1 | MTR_5g062280 | 50.352 | 284 | 138 | 1 | 277 | 560 | 7 | 287 | 1.18e-90 | 288 |
| MS.gene58342.t1 | MTR_3g079320 | 47.475 | 297 | 150 | 3 | 547 | 838 | 11 | 306 | 1.68e-83 | 270 |
| MS.gene58342.t1 | MTR_3g079320 | 48.399 | 281 | 138 | 4 | 6 | 281 | 10 | 288 | 1.04e-78 | 257 |
| MS.gene58342.t1 | MTR_3g079320 | 48.214 | 280 | 138 | 4 | 277 | 551 | 11 | 288 | 4.37e-78 | 255 |
| MS.gene58342.t1 | MTR_8g031850 | 46.918 | 292 | 112 | 3 | 547 | 838 | 7 | 255 | 3.11e-83 | 267 |
| MS.gene58342.t1 | MTR_8g031850 | 45.907 | 281 | 108 | 3 | 1 | 281 | 1 | 237 | 2.22e-76 | 249 |
| MS.gene58342.t1 | MTR_8g031850 | 45.818 | 275 | 105 | 4 | 277 | 551 | 7 | 237 | 5.31e-73 | 239 |
| MS.gene58342.t1 | MTR_7g106800 | 47.440 | 293 | 94 | 3 | 547 | 834 | 9 | 246 | 1.98e-76 | 249 |
| MS.gene58342.t1 | MTR_7g106800 | 44.000 | 300 | 106 | 4 | 4 | 297 | 6 | 249 | 8.91e-67 | 223 |
| MS.gene58342.t1 | MTR_7g106800 | 44.781 | 297 | 102 | 5 | 277 | 567 | 9 | 249 | 1.26e-66 | 223 |
| MS.gene58342.t1 | MTR_5g083430 | 56.140 | 171 | 75 | 0 | 668 | 838 | 14 | 184 | 4.24e-65 | 216 |
| MS.gene58342.t1 | MTR_5g083430 | 54.375 | 160 | 71 | 1 | 128 | 287 | 13 | 170 | 2.79e-56 | 191 |
| MS.gene58342.t1 | MTR_5g083430 | 55.625 | 160 | 69 | 1 | 398 | 557 | 13 | 170 | 9.34e-56 | 190 |
| MS.gene58342.t1 | MTR_3g098470 | 42.321 | 293 | 132 | 6 | 549 | 830 | 37 | 303 | 2.38e-64 | 218 |
| MS.gene58342.t1 | MTR_3g098470 | 40.418 | 287 | 133 | 5 | 6 | 281 | 34 | 293 | 2.21e-58 | 202 |
| MS.gene58342.t1 | MTR_3g098470 | 40.845 | 284 | 130 | 5 | 279 | 551 | 37 | 293 | 2.27e-57 | 199 |
| MS.gene58342.t1 | MTR_1g031180 | 38.567 | 293 | 143 | 5 | 549 | 830 | 42 | 308 | 3.56e-54 | 191 |
| MS.gene58342.t1 | MTR_1g031180 | 38.732 | 284 | 136 | 6 | 279 | 551 | 42 | 298 | 3.00e-51 | 182 |
| MS.gene58342.t1 | MTR_1g031180 | 37.979 | 287 | 140 | 7 | 6 | 281 | 39 | 298 | 2.23e-50 | 180 |
| MS.gene58342.t1 | MTR_8g096060 | 36.860 | 293 | 145 | 6 | 549 | 830 | 39 | 302 | 1.27e-50 | 180 |
| MS.gene58342.t1 | MTR_8g096060 | 36.237 | 287 | 142 | 7 | 6 | 281 | 36 | 292 | 9.99e-48 | 172 |
| MS.gene58342.t1 | MTR_8g096060 | 35.211 | 284 | 143 | 7 | 279 | 551 | 39 | 292 | 3.06e-44 | 162 |
| MS.gene58342.t1 | MTR_4g087460 | 36.120 | 299 | 150 | 8 | 549 | 831 | 24 | 297 | 9.74e-48 | 172 |
| MS.gene58342.t1 | MTR_4g087460 | 37.143 | 280 | 135 | 7 | 279 | 543 | 24 | 277 | 1.94e-45 | 166 |
| MS.gene58342.t1 | MTR_4g087460 | 36.749 | 283 | 138 | 8 | 6 | 273 | 21 | 277 | 2.43e-45 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58342.t1 | AT3G61220 | 58.983 | 295 | 113 | 5 | 547 | 838 | 7 | 296 | 1.21e-115 | 353 |
| MS.gene58342.t1 | AT3G61220 | 58.657 | 283 | 109 | 4 | 1 | 281 | 1 | 277 | 4.59e-109 | 336 |
| MS.gene58342.t1 | AT3G61220 | 55.957 | 277 | 114 | 4 | 277 | 551 | 7 | 277 | 5.66e-104 | 323 |
| MS.gene58342.t1 | AT3G61220 | 58.983 | 295 | 113 | 5 | 547 | 838 | 14 | 303 | 2.24e-115 | 353 |
| MS.gene58342.t1 | AT3G61220 | 58.845 | 277 | 106 | 4 | 7 | 281 | 14 | 284 | 3.01e-107 | 332 |
| MS.gene58342.t1 | AT3G61220 | 55.957 | 277 | 114 | 4 | 277 | 551 | 14 | 284 | 1.11e-103 | 322 |
| MS.gene58342.t1 | AT3G61220 | 58.983 | 295 | 113 | 5 | 547 | 838 | 38 | 327 | 1.18e-114 | 352 |
| MS.gene58342.t1 | AT3G61220 | 58.845 | 277 | 106 | 4 | 7 | 281 | 38 | 308 | 1.26e-106 | 331 |
| MS.gene58342.t1 | AT3G61220 | 55.957 | 277 | 114 | 4 | 277 | 551 | 38 | 308 | 4.57e-103 | 322 |
| MS.gene58342.t1 | AT1G01800 | 56.376 | 298 | 124 | 5 | 543 | 838 | 2 | 295 | 3.21e-111 | 342 |
| MS.gene58342.t1 | AT1G01800 | 55.957 | 277 | 116 | 4 | 7 | 281 | 5 | 277 | 6.42e-100 | 312 |
| MS.gene58342.t1 | AT1G01800 | 53.819 | 288 | 126 | 5 | 277 | 561 | 5 | 288 | 3.24e-99 | 310 |
| MS.gene58342.t1 | AT2G24190 | 56.271 | 295 | 121 | 5 | 547 | 838 | 7 | 296 | 1.39e-108 | 335 |
| MS.gene58342.t1 | AT2G24190 | 55.124 | 283 | 119 | 4 | 1 | 281 | 1 | 277 | 7.61e-100 | 312 |
| MS.gene58342.t1 | AT2G24190 | 53.791 | 277 | 120 | 4 | 277 | 551 | 7 | 277 | 3.28e-99 | 310 |
| MS.gene58342.t1 | AT2G24190 | 55.410 | 305 | 127 | 6 | 538 | 838 | 2 | 301 | 1.50e-108 | 335 |
| MS.gene58342.t1 | AT2G24190 | 53.791 | 277 | 120 | 4 | 277 | 551 | 12 | 282 | 5.56e-99 | 310 |
| MS.gene58342.t1 | AT2G24190 | 55.596 | 277 | 115 | 4 | 7 | 281 | 12 | 282 | 1.62e-98 | 309 |
| MS.gene58342.t1 | AT1G01800 | 54.067 | 209 | 93 | 2 | 630 | 838 | 55 | 260 | 6.19e-74 | 242 |
| MS.gene58342.t1 | AT1G01800 | 52.604 | 192 | 87 | 2 | 90 | 281 | 55 | 242 | 1.74e-63 | 214 |
| MS.gene58342.t1 | AT1G01800 | 49.754 | 203 | 97 | 3 | 360 | 561 | 55 | 253 | 4.72e-63 | 213 |
| MS.gene58342.t1 | AT5G51030 | 37.543 | 293 | 146 | 6 | 549 | 830 | 40 | 306 | 2.29e-56 | 196 |
| MS.gene58342.t1 | AT5G51030 | 36.842 | 285 | 140 | 6 | 279 | 551 | 40 | 296 | 1.02e-51 | 184 |
| MS.gene58342.t1 | AT5G51030 | 36.458 | 288 | 145 | 7 | 5 | 281 | 36 | 296 | 7.93e-51 | 181 |
| MS.gene58342.t1 | AT3G59710 | 35.540 | 287 | 155 | 6 | 549 | 831 | 21 | 281 | 4.17e-48 | 173 |
| MS.gene58342.t1 | AT3G59710 | 35.793 | 271 | 144 | 5 | 6 | 273 | 18 | 261 | 4.97e-48 | 172 |
| MS.gene58342.t1 | AT3G59710 | 38.060 | 268 | 136 | 7 | 279 | 543 | 21 | 261 | 7.13e-47 | 169 |
| MS.gene58342.t1 | AT3G59710 | 35.889 | 287 | 154 | 7 | 549 | 831 | 31 | 291 | 4.96e-48 | 173 |
| MS.gene58342.t1 | AT3G59710 | 35.793 | 271 | 144 | 5 | 6 | 273 | 28 | 271 | 1.11e-47 | 172 |
| MS.gene58342.t1 | AT3G59710 | 38.060 | 268 | 136 | 7 | 279 | 543 | 31 | 271 | 8.64e-47 | 169 |
| MS.gene58342.t1 | AT5G61830 | 38.163 | 283 | 140 | 4 | 549 | 820 | 39 | 297 | 5.76e-48 | 173 |
| MS.gene58342.t1 | AT5G61830 | 38.328 | 287 | 141 | 6 | 6 | 281 | 36 | 297 | 3.14e-46 | 168 |
| MS.gene58342.t1 | AT5G61830 | 37.324 | 284 | 142 | 5 | 279 | 551 | 39 | 297 | 5.87e-45 | 164 |
| MS.gene58342.t1 | AT5G61830 | 38.163 | 283 | 140 | 4 | 549 | 820 | 39 | 297 | 5.76e-48 | 173 |
| MS.gene58342.t1 | AT5G61830 | 38.328 | 287 | 141 | 6 | 6 | 281 | 36 | 297 | 3.14e-46 | 168 |
| MS.gene58342.t1 | AT5G61830 | 37.324 | 284 | 142 | 5 | 279 | 551 | 39 | 297 | 5.87e-45 | 164 |
| MS.gene58342.t1 | AT3G59710 | 35.889 | 287 | 154 | 7 | 549 | 831 | 50 | 310 | 1.47e-47 | 172 |
| MS.gene58342.t1 | AT3G59710 | 35.793 | 271 | 144 | 5 | 6 | 273 | 47 | 290 | 2.20e-47 | 172 |
| MS.gene58342.t1 | AT3G59710 | 38.060 | 268 | 136 | 7 | 279 | 543 | 50 | 290 | 2.83e-46 | 169 |
Find 162 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTCAAATAAATTATTA+TGG | 0.154385 | 8.2:+66126119 | MS.gene58342:CDS |
| ATTTGGCAAACTTGATATTC+TGG | 0.218733 | 8.2:+66124786 | MS.gene58342:CDS |
| ATTTGGCAAACTTGATATTC+TGG | 0.218733 | 8.2:+66135485 | MS.gene58342:CDS |
| GGAACCTTCTTCAAAATCTT+TGG | 0.239520 | 8.2:-66137063 | None:intergenic |
| TGCATTCAAATAAATTACTA+TGG | 0.252132 | 8.2:+66136572 | MS.gene58342:CDS |
| CATCTATTCTCTCTTCTGTT+AGG | 0.258784 | 8.2:-66126787 | None:intergenic |
| CATCTATTCTCTCTTCTGTT+AGG | 0.258784 | 8.2:-66137022 | None:intergenic |
| ATTTGGCAAACTTGACATTC+TGG | 0.281094 | 8.2:+66130515 | MS.gene58342:CDS |
| AAAAGGATGGCCAAGATATT+TGG | 0.282941 | 8.2:+66137094 | MS.gene58342:CDS |
| AGGCCAACCTTTCCTTTCTA+AGG | 0.298959 | 8.2:-66126849 | None:intergenic |
| AGGCCAACCATTTCTTTCTA+AGG | 0.304582 | 8.2:-66132223 | None:intergenic |
| GAGAATAGATGAAGTAATAA+AGG | 0.307579 | 8.2:+66126799 | MS.gene58342:CDS |
| GAAAAGAGGTCTTCATGATT+TGG | 0.316163 | 8.2:+66135359 | MS.gene58342:CDS |
| TGCATAGAAATAAATTATTA+TGG | 0.332606 | 8.2:+66131654 | MS.gene58342:CDS |
| CTCAGGTATGCAGTGGTGAC+TGG | 0.352288 | 8.2:+66135255 | MS.gene58342:intron |
| CTCAGGTATGCAGTGGTGAC+TGG | 0.354241 | 8.2:+66124556 | MS.gene58342:intron |
| ACCTGTATTAGCAGTTATAT+CGG | 0.361405 | 8.2:-66126987 | None:intergenic |
| ACCTGTATTAGCAGTTATAT+CGG | 0.361405 | 8.2:-66132361 | None:intergenic |
| GGAAGCAGTGCTAACTTAAC+AGG | 0.370117 | 8.2:-66137275 | None:intergenic |
| ATTAGTTTGTATCGAATGAA+TGG | 0.371859 | 8.2:+66136970 | MS.gene58342:intron |
| GCATTCAAATAAATTATTAT+GGG | 0.371941 | 8.2:+66126120 | MS.gene58342:CDS |
| ATCAGTATGTATCAAATGAA+TGG | 0.381467 | 8.2:+66126735 | MS.gene58342:intron |
| GAAAAGAGGTCTTCATGCTT+TGG | 0.381657 | 8.2:+66124660 | MS.gene58342:CDS |
| GAAAAGAGGTCTTCATGCTT+TGG | 0.381657 | 8.2:+66130389 | MS.gene58342:CDS |
| ATTTCTATGCATTCTTTAGC+TGG | 0.384474 | 8.2:-66131642 | None:intergenic |
| GGATTGGGTTAGAGATAGTA+AGG | 0.386961 | 8.2:+66130319 | MS.gene58342:CDS |
| ATAGTTGAAGGAGGGGAAGA+AGG | 0.389200 | 8.2:-66126163 | None:intergenic |
| GGAAGTAGTGCTAACCTAAC+AGG | 0.389438 | 8.2:-66127040 | None:intergenic |
| GGAAGGAGTGCTAACCTAAC+AGG | 0.393468 | 8.2:-66132414 | None:intergenic |
| GTTAACAATGCTGGGATTAA+CGG | 0.398592 | 8.2:+66136099 | MS.gene58342:CDS |
| ACTGGAGCAAACAAAGGAAT+TGG | 0.400758 | 8.2:+66124574 | MS.gene58342:CDS |
| TTGGAAACACTCAAAGCATC+TGG | 0.405378 | 8.2:+66130408 | MS.gene58342:CDS |
| ACCAGTATTAGCAGTGATAT+CGG | 0.407089 | 8.2:-66137222 | None:intergenic |
| TGTAGGTATGCAGTGGTGAC+TGG | 0.411576 | 8.2:+66130285 | MS.gene58342:intron |
| CTTCAGCAATCATTACGAAC+GGG | 0.413244 | 8.2:+66130862 | MS.gene58342:CDS |
| ACTGGATCGAACAAAGGAAT+TGG | 0.415304 | 8.2:+66135273 | MS.gene58342:CDS |
| AACATCACTGAAGACTCCTT+TGG | 0.418064 | 8.2:-66126759 | None:intergenic |
| GTCAAGTACTCATTACGAAC+GGG | 0.428471 | 8.2:+66136151 | MS.gene58342:CDS |
| CTTCCTAATGGTAGTCCATC+TGG | 0.430212 | 8.2:+66137293 | MS.gene58342:CDS |
| AAGTTAGCACTGCTTCCTAA+TGG | 0.431073 | 8.2:+66137281 | MS.gene58342:CDS |
| ATTGCATAGGTTAACAATGC+TGG | 0.431199 | 8.2:+66125582 | MS.gene58342:intron |
| AGAAGCTTTAGCAACAATAT+AGG | 0.431311 | 8.2:-66126885 | None:intergenic |
| GAATTGGATTTGAGATAGTA+AGG | 0.438309 | 8.2:+66135289 | MS.gene58342:CDS |
| ACACAATTGATGCAGAAGTT+AGG | 0.439194 | 8.2:-66137179 | None:intergenic |
| TAGAAAGAAATGGTTGGCCT+AGG | 0.439858 | 8.2:+66132226 | MS.gene58342:CDS |
| ACCGATATCACTGCTAATAC+TGG | 0.440027 | 8.2:+66137221 | MS.gene58342:CDS |
| AGAAGCTTTAGAAACAATAT+AGG | 0.440409 | 8.2:-66132259 | None:intergenic |
| CATCTGGCGTTTACTATATC+CGG | 0.447301 | 8.2:+66137309 | MS.gene58342:CDS |
| TGCTGCAAGTGTAGCTTCTC+TGG | 0.448719 | 8.2:+66124744 | MS.gene58342:CDS |
| AAGGTGCTGCTCATCCTGTT+AGG | 0.448829 | 8.2:+66127026 | MS.gene58342:CDS |
| TTGAAGAAGGGTCCTTAGAA+AGG | 0.448969 | 8.2:+66126837 | MS.gene58342:CDS |
| ACTAAACTAATCATGACAAA+GGG | 0.449399 | 8.2:+66125642 | MS.gene58342:CDS |
| TGCATCAATTGTGTTTGTCC+TGG | 0.452387 | 8.2:+66132327 | MS.gene58342:CDS |
| GTATAGGCAGCCAAATATCT+TGG | 0.452873 | 8.2:-66137104 | None:intergenic |
| ACTGGATCAAACAAAGGGAT+TGG | 0.459468 | 8.2:+66130303 | MS.gene58342:CDS |
| ACACAATTGATGCAGAAGTT+AGG | 0.460671 | 8.2:-66126944 | None:intergenic |
| TCGGTGAATCAGATAATTGT+AGG | 0.460709 | 8.2:-66131710 | None:intergenic |
| TTGGAAACACTCAAAACCTC+TGG | 0.460844 | 8.2:+66124679 | MS.gene58342:CDS |
| AAGGTGCTGCAAATCCTGTT+AGG | 0.463404 | 8.2:+66132400 | MS.gene58342:CDS |
| AGAAGCTTTAGCAACAGTAT+AGG | 0.466477 | 8.2:-66137120 | None:intergenic |
| GTTAAGCAGTTAGCTTCAGC+TGG | 0.466868 | 8.2:+66124607 | MS.gene58342:CDS |
| TGCATCAATTGTGTGTGCCC+TGG | 0.470212 | 8.2:+66126953 | MS.gene58342:CDS |
| GTCAATGTATCATCAGGATT+GGG | 0.471976 | 8.2:+66136656 | MS.gene58342:CDS |
| TCAGTATGTATCAAATGAAT+GGG | 0.473036 | 8.2:+66126736 | MS.gene58342:intron |
| AGTCAATGTATCATCAGGAT+TGG | 0.473356 | 8.2:+66136655 | MS.gene58342:CDS |
| GTTAACAATGCTGGGATTGG+TGG | 0.475719 | 8.2:+66125591 | MS.gene58342:CDS |
| TGCATCAATTGTGTTTGCCC+TGG | 0.476427 | 8.2:+66137188 | MS.gene58342:CDS |
| GAAGGGTCCTTAGAAAGAAA+TGG | 0.479473 | 8.2:+66132216 | MS.gene58342:CDS |
| ACGCCAGATGGACTACCATT+AGG | 0.483185 | 8.2:-66137296 | None:intergenic |
| GAAGGTTCCTTAGAAAGAAA+AGG | 0.484128 | 8.2:+66137077 | MS.gene58342:CDS |
| AGAGTAGTCAATGTATCATC+AGG | 0.484821 | 8.2:+66136650 | MS.gene58342:CDS |
| GATGATACATTGACTACTCT+TGG | 0.488410 | 8.2:-66126194 | None:intergenic |
| GTAAGGCAATTAGCTTCAGC+TGG | 0.490726 | 8.2:+66135306 | MS.gene58342:CDS |
| TCCATATAAAATCATCAAAA+CGG | 0.490861 | 8.2:-66137344 | None:intergenic |
| TTGCATAGGTTAACAATGCT+GGG | 0.491383 | 8.2:+66125583 | MS.gene58342:intron |
| GCATTCAAATAAATTACTAT+GGG | 0.492881 | 8.2:+66136573 | MS.gene58342:CDS |
| TTAGTTTGTATCGAATGAAT+GGG | 0.494173 | 8.2:+66136971 | MS.gene58342:intron |
| CTGGATCAAACAAAGGGATT+GGG | 0.494569 | 8.2:+66130304 | MS.gene58342:CDS |
| ATTGTGTAGGTTAACAATGC+TGG | 0.496371 | 8.2:+66136090 | MS.gene58342:intron |
| GTAAGGCAGTTAGCTTCAGC+TGG | 0.501587 | 8.2:+66130336 | MS.gene58342:CDS |
| TTGGTGAATCAGATAGTTGA+AGG | 0.507829 | 8.2:-66126175 | None:intergenic |
| GATGATACATTGACTACTCT+TGG | 0.507830 | 8.2:-66136647 | None:intergenic |
| ATCAGGATTGGGCAAGATAG+AGG | 0.513958 | 8.2:+66136667 | MS.gene58342:CDS |
| ACCGATATAACTGCTAATAC+AGG | 0.514553 | 8.2:+66126986 | MS.gene58342:CDS |
| ACCGATATAACTGCTAATAC+AGG | 0.514553 | 8.2:+66132360 | MS.gene58342:CDS |
| ATAACTGAAGGAGGGGAAGA+AGG | 0.515357 | 8.2:-66136616 | None:intergenic |
| TTGGAGACACTCAAAGCCTC+TGG | 0.517200 | 8.2:+66135378 | MS.gene58342:CDS |
| ATAATTGTAGGAGGGGAAGA+AGG | 0.520779 | 8.2:-66131698 | None:intergenic |
| GTTAACAATGCTGGGATTAG+CGG | 0.521032 | 8.2:+66130810 | MS.gene58342:CDS |
| CGGATATAGTAAACGCCAGA+TGG | 0.522787 | 8.2:-66137308 | None:intergenic |
| TAGAAAGGAAAGGTTGGCCT+AGG | 0.524315 | 8.2:+66126852 | MS.gene58342:CDS |
| ATTGTGCAGGTTAACAATGC+TGG | 0.525506 | 8.2:+66130801 | MS.gene58342:intron |
| GTTAGCTTCAGCTGGAATCA+AGG | 0.525651 | 8.2:+66130344 | MS.gene58342:CDS |
| GTATCAAATGAGTGGGCAAA+AGG | 0.528220 | 8.2:+66132117 | MS.gene58342:CDS |
| GTATCGAATGAATGGGCTAA+AGG | 0.528870 | 8.2:+66136978 | MS.gene58342:CDS |
| ACACAATTGATGCAGAAACT+AGG | 0.532163 | 8.2:-66132318 | None:intergenic |
| CACTAAACTAATCATGACAA+AGG | 0.543900 | 8.2:+66125641 | MS.gene58342:CDS |
| CTTTGCAGTAATTCTTGTGT+AGG | 0.546772 | 8.2:-66126915 | None:intergenic |
| GAATCAGATAATTGTAGGAG+GGG | 0.547037 | 8.2:-66131705 | None:intergenic |
| TAGGTTAACAATGCTGGGAT+TGG | 0.549637 | 8.2:+66125588 | MS.gene58342:intron |
| CATTCAAATAAATTATTATG+GGG | 0.549783 | 8.2:+66126121 | MS.gene58342:CDS |
| TTGTGTAGGTTAACAATGCT+GGG | 0.550993 | 8.2:+66136091 | MS.gene58342:intron |
| GGTATCTTCAAAGTTGAAGA+AGG | 0.552996 | 8.2:+66127007 | MS.gene58342:CDS |
| GAATCAGATAGTTGAAGGAG+GGG | 0.553764 | 8.2:-66126170 | None:intergenic |
| GATGACACATTGACCACTCT+CGG | 0.556968 | 8.2:-66131729 | None:intergenic |
| ACTGCAAGAAATGAGAAAAG+AGG | 0.559420 | 8.2:+66124646 | MS.gene58342:CDS |
| AAAACGGATAGACTTCATTC+CGG | 0.560354 | 8.2:-66137328 | None:intergenic |
| ATGAGCAGCACCTTCTTCTA+CGG | 0.562549 | 8.2:-66137252 | None:intergenic |
| ACAGCAAGAGATGAGAAAAG+AGG | 0.563297 | 8.2:+66130375 | MS.gene58342:CDS |
| ACAGCAAGAGATGAGAAAAG+AGG | 0.563297 | 8.2:+66135345 | MS.gene58342:CDS |
| GTCAATGTATCATCGAGAGC+AGG | 0.564594 | 8.2:+66126203 | MS.gene58342:CDS |
| TGAATCAGATAGTTGAAGGA+GGG | 0.569558 | 8.2:-66126171 | None:intergenic |
| GGTATCTTCACAGCAGAAGA+AGG | 0.571933 | 8.2:+66132381 | MS.gene58342:CDS |
| CCTCTTTCTCAGGTATGCAG+TGG | 0.572591 | 8.2:+66135248 | MS.gene58342:intron |
| GGTCTCTTCACCGTAGAAGA+AGG | 0.573243 | 8.2:+66137242 | MS.gene58342:CDS |
| TTGTGCAGGTTAACAATGCT+GGG | 0.573954 | 8.2:+66130802 | MS.gene58342:intron |
| TCTTCAGCAATCATTACGAA+CGG | 0.574758 | 8.2:+66130861 | MS.gene58342:CDS |
| TGGTGACTGGATCAAACAAA+GGG | 0.587027 | 8.2:+66130298 | MS.gene58342:CDS |
| GAATCAGATAACTGAAGGAG+GGG | 0.588357 | 8.2:-66136623 | None:intergenic |
| GTGAATCAGATAATTGTAGG+AGG | 0.590899 | 8.2:-66131707 | None:intergenic |
| TTGGTGAATCAGATAACTGA+AGG | 0.592333 | 8.2:-66136628 | None:intergenic |
| AGATGGAAGAATATACAGAA+AGG | 0.592841 | 8.2:+66124431 | None:intergenic |
| ATTATCTGATTCACCGAGAG+TGG | 0.593928 | 8.2:+66131716 | MS.gene58342:CDS |
| GGTCCTTAGAAAGGAAAGGT+TGG | 0.595012 | 8.2:+66126846 | MS.gene58342:CDS |
| ATCAGTGTGTATCAAATGAG+TGG | 0.595416 | 8.2:+66132109 | MS.gene58342:intron |
| ATATAGGCAGCTGCAAACCT+AGG | 0.596713 | 8.2:-66132243 | None:intergenic |
| TCAGTGTGTATCAAATGAGT+GGG | 0.601615 | 8.2:+66132110 | MS.gene58342:intron |
| TATCTGATGAAGAATTGAGA+AGG | 0.601830 | 8.2:+66131598 | MS.gene58342:CDS |
| TGAATCAGATAATTGTAGGA+GGG | 0.607788 | 8.2:-66131706 | None:intergenic |
| ATCTGATGAAGAATTGAGAA+GGG | 0.607999 | 8.2:+66131599 | MS.gene58342:CDS |
| ATCTGATGAAGAATCGAGAA+GGG | 0.610949 | 8.2:+66126064 | MS.gene58342:CDS |
| ATATAGGCAGCTAAATACCT+AGG | 0.613579 | 8.2:-66126869 | None:intergenic |
| CTAAACTAATCATGACAAAG+GGG | 0.614957 | 8.2:+66125643 | MS.gene58342:CDS |
| TCTCTTCCTCAGGTATGCAG+TGG | 0.615906 | 8.2:+66124549 | MS.gene58342:intron |
| GTATCAAATGAATGGGCCAA+AGG | 0.616546 | 8.2:+66126743 | MS.gene58342:CDS |
| AGTCAAGTACTCATTACGAA+CGG | 0.617703 | 8.2:+66136150 | MS.gene58342:CDS |
| TGAATCAGATAACTGAAGGA+GGG | 0.621361 | 8.2:-66136624 | None:intergenic |
| TATCTGATGAAGAATCGAGA+AGG | 0.629789 | 8.2:+66126063 | MS.gene58342:CDS |
| GAAGGGTCCTTAGAAAGGAA+AGG | 0.633044 | 8.2:+66126842 | MS.gene58342:CDS |
| GTGAATCAGATAACTGAAGG+AGG | 0.634353 | 8.2:-66136625 | None:intergenic |
| GTGAATCAGATAGTTGAAGG+AGG | 0.640969 | 8.2:-66126172 | None:intergenic |
| GTGGTGACTGGATCAAACAA+AGG | 0.640993 | 8.2:+66130297 | MS.gene58342:CDS |
| AATCTGATGAGGAATTGAGA+AGG | 0.642308 | 8.2:+66136516 | MS.gene58342:CDS |
| GGTCCTTAGAAAGAAATGGT+TGG | 0.645706 | 8.2:+66132220 | MS.gene58342:CDS |
| ATCGAGAGCAGGCACGATGG+AGG | 0.645992 | 8.2:+66126214 | MS.gene58342:CDS |
| GTTCCTTAGAAAGAAAAGGA+TGG | 0.646946 | 8.2:+66137081 | MS.gene58342:CDS |
| CTTGCAGGCACAATCTGATG+AGG | 0.653126 | 8.2:+66136505 | MS.gene58342:intron |
| GTGGTGACTGGATCGAACAA+AGG | 0.656701 | 8.2:+66135267 | MS.gene58342:CDS |
| CATTCAAATAAATTACTATG+GGG | 0.662211 | 8.2:+66136574 | MS.gene58342:CDS |
| GTGGTGACTGGAGCAAACAA+AGG | 0.662474 | 8.2:+66124568 | MS.gene58342:CDS |
| AGCTTCAGCTGGAATCAAAG+TGG | 0.664366 | 8.2:+66124618 | MS.gene58342:CDS |
| AGCTTCAGCTGGAATCAAAG+TGG | 0.664366 | 8.2:+66135317 | MS.gene58342:CDS |
| TGCAAGTGTAGCTTCTCTGG+CGG | 0.669030 | 8.2:+66124747 | MS.gene58342:CDS |
| AACAAAATCAGATAAACCAG+AGG | 0.674498 | 8.2:-66124695 | None:intergenic |
| AACAAAATCAGAAAGACCAG+AGG | 0.674583 | 8.2:-66135394 | None:intergenic |
| ATCATCGAGAGCAGGCACGA+TGG | 0.681338 | 8.2:+66126211 | MS.gene58342:CDS |
| TTCAGCAATCATTACGAACG+GGG | 0.681419 | 8.2:+66130863 | MS.gene58342:CDS |
| TAAACTAATCATGACAAAGG+GGG | 0.699761 | 8.2:+66125644 | MS.gene58342:CDS |
| GGTGGAATTGAAATCAACGA+CGG | 0.720856 | 8.2:+66125609 | MS.gene58342:CDS |
| AGCTTCAGCTGGAATCAAGG+TGG | 0.722149 | 8.2:+66130347 | MS.gene58342:CDS |
| TAAGGTGTGTAGGTATGCAG+TGG | 0.735423 | 8.2:+66130278 | MS.gene58342:intron |
| TCAAGTACTCATTACGAACG+GGG | 0.752274 | 8.2:+66136152 | MS.gene58342:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 66124433 | 66137352 | 66124433 | ID=MS.gene58342 |
| chr8.2 | mRNA | 66124433 | 66137352 | 66124433 | ID=MS.gene58342.t1;Parent=MS.gene58342 |
| chr8.2 | exon | 66124433 | 66124452 | 66124433 | ID=MS.gene58342.t1.exon1;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66124433 | 66124452 | 66124433 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66124561 | 66124807 | 66124561 | ID=MS.gene58342.t1.exon2;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66124561 | 66124807 | 66124561 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66125591 | 66125665 | 66125591 | ID=MS.gene58342.t1.exon3;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66125591 | 66125665 | 66125591 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66126056 | 66126235 | 66126056 | ID=MS.gene58342.t1.exon4;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66126056 | 66126235 | 66126056 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66126740 | 66127047 | 66126740 | ID=MS.gene58342.t1.exon5;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66126740 | 66127047 | 66126740 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66130290 | 66130536 | 66130290 | ID=MS.gene58342.t1.exon6;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66130290 | 66130536 | 66130290 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66130810 | 66130884 | 66130810 | ID=MS.gene58342.t1.exon7;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66130810 | 66130884 | 66130810 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66131591 | 66131770 | 66131591 | ID=MS.gene58342.t1.exon8;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66131591 | 66131770 | 66131591 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66132114 | 66132421 | 66132114 | ID=MS.gene58342.t1.exon9;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66132114 | 66132421 | 66132114 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66135260 | 66135506 | 66135260 | ID=MS.gene58342.t1.exon10;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66135260 | 66135506 | 66135260 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66136099 | 66136173 | 66136099 | ID=MS.gene58342.t1.exon11;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66136099 | 66136173 | 66136099 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66136512 | 66136688 | 66136512 | ID=MS.gene58342.t1.exon12;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66136512 | 66136688 | 66136512 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| chr8.2 | exon | 66136975 | 66137352 | 66136975 | ID=MS.gene58342.t1.exon13;Parent=MS.gene58342.t1 |
| chr8.2 | CDS | 66136975 | 66137352 | 66136975 | ID=cds.MS.gene58342.t1;Parent=MS.gene58342.t1 |
| Gene Sequence |
| Protein sequence |
