Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58589.t1 | XP_003589014.1 | 89.4 | 322 | 33 | 1 | 1 | 322 | 1 | 321 | 4.50E-163 | 583.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58589.t1 | Q9FFJ8 | 25.3 | 308 | 210 | 5 | 15 | 322 | 160 | 447 | 8.0e-20 | 99.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58589.t1 | G7IB81 | 89.4 | 322 | 33 | 1 | 1 | 322 | 1 | 321 | 3.2e-163 | 583.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene58589 | 0.815377 | 9.54E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene58589 | 0.823805 | 1.14E-53 | -1.69E-46 |
| MS.gene052447 | MS.gene58589 | 0.824045 | 9.98E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene58589 | 0.81107 | 8.41E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene58589 | 0.821606 | 3.69E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene58589 | 0.810795 | 9.65E-51 | -1.69E-46 |
| MS.gene056272 | MS.gene58589 | 0.984107 | 1.32E-159 | -1.69E-46 |
| MS.gene057598 | MS.gene58589 | 0.813215 | 2.86E-51 | -1.69E-46 |
| MS.gene058008 | MS.gene58589 | 0.800194 | 1.62E-48 | -1.69E-46 |
| MS.gene060634 | MS.gene58589 | -0.815866 | 7.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58589.t1 | MTR_1g016370 | 91.615 | 322 | 26 | 1 | 1 | 322 | 1 | 321 | 0.0 | 604 |
| MS.gene58589.t1 | MTR_4g061370 | 87.578 | 322 | 21 | 2 | 1 | 322 | 1013 | 1315 | 0.0 | 550 |
| MS.gene58589.t1 | MTR_5g055390 | 77.124 | 306 | 26 | 2 | 17 | 322 | 20 | 281 | 4.81e-161 | 451 |
| MS.gene58589.t1 | MTR_8g054100 | 90.336 | 238 | 19 | 1 | 1 | 238 | 1 | 234 | 2.26e-148 | 417 |
| MS.gene58589.t1 | MTR_2g018710 | 63.272 | 324 | 113 | 4 | 2 | 321 | 1 | 322 | 5.53e-144 | 409 |
| MS.gene58589.t1 | MTR_2g086350 | 62.963 | 324 | 114 | 4 | 2 | 321 | 1 | 322 | 1.01e-142 | 406 |
| MS.gene58589.t1 | MTR_1g080080 | 62.654 | 324 | 115 | 4 | 2 | 321 | 1 | 322 | 1.57e-142 | 405 |
| MS.gene58589.t1 | MTR_3g110155 | 57.012 | 328 | 134 | 2 | 1 | 321 | 1 | 328 | 7.31e-130 | 374 |
| MS.gene58589.t1 | MTR_8g006585 | 57.012 | 328 | 134 | 2 | 1 | 321 | 1 | 328 | 7.31e-130 | 374 |
| MS.gene58589.t1 | MTR_8g006695 | 57.012 | 328 | 134 | 2 | 1 | 321 | 1 | 328 | 7.31e-130 | 374 |
| MS.gene58589.t1 | MTR_5g054570 | 59.259 | 324 | 108 | 5 | 2 | 321 | 1 | 304 | 8.11e-129 | 370 |
| MS.gene58589.t1 | MTR_2g435870 | 55.556 | 306 | 111 | 3 | 19 | 321 | 5 | 288 | 2.58e-117 | 340 |
| MS.gene58589.t1 | MTR_4g046743 | 51.393 | 323 | 104 | 5 | 2 | 321 | 1 | 273 | 1.68e-101 | 300 |
| MS.gene58589.t1 | MTR_2g054730 | 85.211 | 142 | 21 | 0 | 181 | 322 | 11 | 152 | 1.62e-78 | 236 |
| MS.gene58589.t1 | MTR_2g436180 | 48.684 | 228 | 77 | 3 | 100 | 321 | 1 | 194 | 1.15e-62 | 197 |
| MS.gene58589.t1 | MTR_7g029320 | 44.867 | 263 | 84 | 6 | 2 | 261 | 1 | 205 | 4.85e-60 | 191 |
| MS.gene58589.t1 | MTR_3g023200 | 85.882 | 85 | 12 | 0 | 1 | 85 | 1 | 85 | 1.81e-46 | 152 |
| MS.gene58589.t1 | MTR_2g436540 | 41.629 | 221 | 111 | 6 | 119 | 321 | 10 | 230 | 1.91e-44 | 152 |
| MS.gene58589.t1 | MTR_3g448480 | 49.686 | 159 | 48 | 4 | 9 | 164 | 8 | 137 | 1.04e-39 | 136 |
| MS.gene58589.t1 | MTR_0961s0020 | 70.930 | 86 | 22 | 2 | 122 | 206 | 5 | 88 | 3.66e-32 | 116 |
| MS.gene58589.t1 | MTR_1g073440 | 39.437 | 142 | 85 | 1 | 21 | 161 | 15 | 156 | 4.60e-30 | 116 |
| MS.gene58589.t1 | MTR_4g043620 | 38.750 | 160 | 94 | 2 | 2 | 161 | 1 | 156 | 5.27e-29 | 113 |
| MS.gene58589.t1 | MTR_8g105430 | 34.595 | 185 | 107 | 3 | 12 | 183 | 31 | 214 | 2.02e-28 | 110 |
| MS.gene58589.t1 | MTR_5g018260 | 28.165 | 316 | 197 | 9 | 22 | 315 | 16 | 323 | 2.11e-26 | 107 |
| MS.gene58589.t1 | MTR_8g468830 | 28.254 | 315 | 197 | 9 | 21 | 319 | 14 | 315 | 4.49e-26 | 105 |
| MS.gene58589.t1 | MTR_8g069895 | 34.884 | 172 | 108 | 2 | 2 | 173 | 1 | 168 | 9.70e-26 | 104 |
| MS.gene58589.t1 | MTR_3g070080 | 38.158 | 152 | 85 | 2 | 22 | 173 | 248 | 390 | 2.61e-25 | 107 |
| MS.gene58589.t1 | MTR_3g070080 | 28.479 | 309 | 159 | 8 | 12 | 311 | 436 | 691 | 3.31e-25 | 106 |
| MS.gene58589.t1 | MTR_3g070080 | 36.567 | 134 | 78 | 2 | 11 | 144 | 66 | 192 | 2.76e-18 | 85.9 |
| MS.gene58589.t1 | MTR_3g070080 | 38.158 | 152 | 85 | 2 | 22 | 173 | 185 | 327 | 3.01e-25 | 106 |
| MS.gene58589.t1 | MTR_3g070080 | 28.479 | 309 | 159 | 8 | 12 | 311 | 373 | 628 | 4.69e-25 | 105 |
| MS.gene58589.t1 | MTR_3g070080 | 36.567 | 134 | 78 | 2 | 11 | 144 | 3 | 129 | 3.77e-18 | 85.5 |
| MS.gene58589.t1 | MTR_5g022570 | 29.000 | 300 | 181 | 8 | 19 | 312 | 17 | 290 | 3.61e-24 | 100 |
| MS.gene58589.t1 | MTR_2g021280 | 34.375 | 160 | 101 | 2 | 2 | 161 | 1 | 156 | 9.15e-23 | 97.8 |
| MS.gene58589.t1 | MTR_7g056530 | 64.516 | 62 | 22 | 0 | 103 | 164 | 28 | 89 | 9.56e-22 | 88.6 |
| MS.gene58589.t1 | MTR_4g045750 | 59.259 | 81 | 30 | 2 | 138 | 217 | 60 | 138 | 1.40e-20 | 86.7 |
| MS.gene58589.t1 | MTR_7g034425 | 53.247 | 77 | 35 | 1 | 12 | 87 | 85 | 161 | 4.52e-20 | 85.9 |
| MS.gene58589.t1 | MTR_0693s0050 | 39.189 | 148 | 78 | 5 | 18 | 158 | 5 | 147 | 9.01e-20 | 87.4 |
| MS.gene58589.t1 | MTR_1g055365 | 30.147 | 136 | 94 | 1 | 21 | 156 | 21 | 155 | 8.04e-16 | 78.6 |
| MS.gene58589.t1 | MTR_1g055365 | 29.655 | 145 | 99 | 2 | 2 | 146 | 175 | 316 | 3.53e-15 | 76.6 |
| MS.gene58589.t1 | MTR_4g029080 | 41.975 | 81 | 47 | 0 | 81 | 161 | 2 | 82 | 5.37e-15 | 70.1 |
| MS.gene58589.t1 | MTR_7g105890 | 37.391 | 115 | 61 | 4 | 18 | 127 | 5 | 113 | 5.30e-14 | 72.4 |
| MS.gene58589.t1 | MTR_4g128540 | 37.391 | 115 | 61 | 4 | 18 | 127 | 5 | 113 | 9.83e-14 | 71.2 |
| MS.gene58589.t1 | MTR_8g085310 | 25.084 | 299 | 185 | 9 | 25 | 310 | 434 | 706 | 3.08e-13 | 70.9 |
| MS.gene58589.t1 | MTR_8g446440 | 25.490 | 255 | 151 | 10 | 81 | 319 | 2 | 233 | 3.46e-13 | 68.6 |
| MS.gene58589.t1 | MTR_4g128327 | 39.796 | 98 | 49 | 3 | 27 | 119 | 14 | 106 | 3.86e-13 | 65.9 |
| MS.gene58589.t1 | MTR_3g053640 | 25.256 | 293 | 168 | 10 | 25 | 310 | 182 | 430 | 1.19e-12 | 68.6 |
| MS.gene58589.t1 | MTR_4g092300 | 24.590 | 305 | 175 | 11 | 25 | 310 | 556 | 824 | 2.66e-12 | 68.2 |
| MS.gene58589.t1 | MTR_4g051480 | 24.590 | 305 | 175 | 11 | 25 | 310 | 574 | 842 | 2.68e-12 | 68.2 |
| MS.gene58589.t1 | MTR_1g024830 | 22.772 | 303 | 204 | 8 | 19 | 311 | 59 | 341 | 5.52e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58589.t1 | AT5G05800 | 25.237 | 317 | 212 | 6 | 6 | 322 | 156 | 447 | 6.98e-24 | 101 |
| MS.gene58589.t1 | AT5G05800 | 36.036 | 111 | 70 | 1 | 20 | 130 | 2 | 111 | 2.05e-16 | 80.1 |
| MS.gene58589.t1 | AT5G05800 | 25.237 | 317 | 212 | 6 | 6 | 322 | 156 | 447 | 6.98e-24 | 101 |
| MS.gene58589.t1 | AT5G05800 | 36.036 | 111 | 70 | 1 | 20 | 130 | 2 | 111 | 2.05e-16 | 80.1 |
| MS.gene58589.t1 | AT5G05800 | 25.237 | 317 | 212 | 6 | 6 | 322 | 156 | 447 | 6.98e-24 | 101 |
| MS.gene58589.t1 | AT5G05800 | 36.036 | 111 | 70 | 1 | 20 | 130 | 2 | 111 | 2.05e-16 | 80.1 |
| MS.gene58589.t1 | AT5G05800 | 25.237 | 317 | 212 | 6 | 6 | 322 | 156 | 447 | 6.98e-24 | 101 |
| MS.gene58589.t1 | AT5G05800 | 36.036 | 111 | 70 | 1 | 20 | 130 | 2 | 111 | 2.05e-16 | 80.1 |
| MS.gene58589.t1 | AT5G05800 | 25.237 | 317 | 212 | 6 | 6 | 322 | 156 | 447 | 6.98e-24 | 101 |
| MS.gene58589.t1 | AT5G05800 | 36.036 | 111 | 70 | 1 | 20 | 130 | 2 | 111 | 2.05e-16 | 80.1 |
| MS.gene58589.t1 | AT3G11290 | 25.243 | 309 | 190 | 8 | 20 | 310 | 163 | 448 | 1.22e-18 | 86.7 |
| MS.gene58589.t1 | AT3G11290 | 25.243 | 309 | 190 | 8 | 20 | 310 | 163 | 448 | 1.22e-18 | 86.7 |
| MS.gene58589.t1 | AT2G24960 | 29.197 | 137 | 96 | 1 | 8 | 144 | 155 | 290 | 2.13e-15 | 77.4 |
| MS.gene58589.t1 | AT2G24960 | 29.197 | 137 | 96 | 1 | 8 | 144 | 155 | 290 | 2.44e-15 | 77.4 |
| MS.gene58589.t1 | AT2G24960 | 27.273 | 143 | 103 | 1 | 12 | 154 | 314 | 455 | 5.14e-12 | 67.4 |
| MS.gene58589.t1 | AT3G11310 | 33.566 | 143 | 89 | 2 | 20 | 157 | 171 | 312 | 5.68e-13 | 70.1 |
| MS.gene58589.t1 | AT2G19220 | 25.705 | 319 | 199 | 12 | 9 | 317 | 149 | 439 | 4.10e-11 | 63.9 |
| MS.gene58589.t1 | AT4G02210 | 27.673 | 159 | 113 | 2 | 13 | 170 | 2 | 159 | 7.61e-11 | 63.2 |
| MS.gene58589.t1 | AT4G02210 | 27.673 | 159 | 113 | 2 | 13 | 170 | 2 | 159 | 7.61e-11 | 63.2 |
Find 75 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCATGGTATAACTTATT+TGG | 0.134716 | 5.3:-24808925 | MS.gene58589:CDS |
| ACAACTGAATTTGGAAATAT+TGG | 0.148866 | 5.3:-24808522 | MS.gene58589:CDS |
| GTTGGAGCAACAACTGAATT+TGG | 0.213211 | 5.3:-24808531 | MS.gene58589:CDS |
| GGCAGAAAACAAAAGCTAAT+TGG | 0.275009 | 5.3:-24809134 | MS.gene58589:CDS |
| AACTTATTTGGGAGAGAAAC+TGG | 0.287945 | 5.3:-24808913 | MS.gene58589:CDS |
| TTGGGAGAGAAACTGGATTA+GGG | 0.298488 | 5.3:-24808906 | MS.gene58589:CDS |
| TTTGGGAGAGAAACTGGATT+AGG | 0.315464 | 5.3:-24808907 | MS.gene58589:CDS |
| GAAATAAACATCTCCCTTGC+AGG | 0.323302 | 5.3:+24808231 | None:intergenic |
| AAACTTTGTGAAAGCATGTT+TGG | 0.334075 | 5.3:-24809094 | MS.gene58589:CDS |
| GTCCGGTTAAATTATGAAAT+CGG | 0.352053 | 5.3:+24809004 | None:intergenic |
| TGTATTACATACCAGTTGTT+TGG | 0.352266 | 5.3:+24808834 | None:intergenic |
| CAACTGAATTTGGAAATATT+GGG | 0.356640 | 5.3:-24808521 | MS.gene58589:CDS |
| GAGCATGGTATAACTTATTT+GGG | 0.363157 | 5.3:-24808924 | MS.gene58589:CDS |
| TTCAAGGATGTAGTGGCTAA+TGG | 0.366838 | 5.3:-24808672 | MS.gene58589:CDS |
| CAGAGGAGAAGAGGTTGAAT+TGG | 0.373243 | 5.3:-24808197 | MS.gene58589:CDS |
| GTCCGATTTCATAATTTAAC+CGG | 0.374307 | 5.3:-24809006 | MS.gene58589:CDS |
| TTGGTCTCCCACCACTCATC+AGG | 0.410369 | 5.3:+24808853 | None:intergenic |
| GCAGAAAACAAAAGCTAATT+GGG | 0.414370 | 5.3:-24809133 | MS.gene58589:CDS |
| GTTCCTCCTCACCTAGTGAT+GGG | 0.415978 | 5.3:-24809158 | MS.gene58589:CDS |
| GAAAAGTGTGGTTAACTCAT+TGG | 0.420206 | 5.3:+24808692 | None:intergenic |
| AAAAGGGTGGACAAATATTA+GGG | 0.422862 | 5.3:-24809028 | MS.gene58589:CDS |
| ATGATCTTTATTTGCATGCT+CGG | 0.426517 | 5.3:-24808278 | MS.gene58589:CDS |
| GGTTCCTCCTCACCTAGTGA+TGG | 0.438340 | 5.3:-24809159 | MS.gene58589:CDS |
| TTCCTCCTCACCTAGTGATG+GGG | 0.476214 | 5.3:-24809157 | MS.gene58589:CDS |
| AGAAGGCTCAACTAAAGAAT+AGG | 0.479515 | 5.3:-24808972 | MS.gene58589:CDS |
| TCCTCCTCACCTAGTGATGG+GGG | 0.484199 | 5.3:-24809156 | MS.gene58589:CDS |
| GCTGCGGGTAATGCATCTAT+TGG | 0.495551 | 5.3:-24808342 | MS.gene58589:CDS |
| GATGAGGCAGTGGCTGTAGA+AGG | 0.502147 | 5.3:-24808579 | MS.gene58589:CDS |
| AGCCTTCTCATACTTCAGTC+CGG | 0.504703 | 5.3:+24808987 | None:intergenic |
| CGTACCAACACGTTCTCCCT+TGG | 0.505137 | 5.3:+24809062 | None:intergenic |
| TGCTGATGTTTAAGCCTGCA+AGG | 0.517021 | 5.3:-24808245 | MS.gene58589:CDS |
| TGGCTAATGGAGAGTACACT+TGG | 0.522261 | 5.3:-24808659 | MS.gene58589:CDS |
| GCAGTGGCTGTAGAAGGATC+TGG | 0.527670 | 5.3:-24808573 | MS.gene58589:CDS |
| ATGACAGTCTGAGAAAGGAT+TGG | 0.531322 | 5.3:-24808948 | MS.gene58589:CDS |
| CTTCTCCTCTGAACCTCGTA+GGG | 0.535159 | 5.3:+24808209 | None:intergenic |
| GTGGTGGGAGACCAAACAAC+TGG | 0.536532 | 5.3:-24808845 | MS.gene58589:intron |
| TAGGTATGACAGTCTGAGAA+AGG | 0.543958 | 5.3:-24808953 | MS.gene58589:CDS |
| AGGTTAGTCATGAATGGCAG+TGG | 0.548613 | 5.3:+24808153 | None:intergenic |
| TCACACAGGTTAGTCATGAA+TGG | 0.548759 | 5.3:+24808147 | None:intergenic |
| TATTTCCCTACGAGGTTCAG+AGG | 0.555424 | 5.3:-24808214 | MS.gene58589:CDS |
| GGAGAGAAACTGGATTAGGG+TGG | 0.557144 | 5.3:-24808903 | MS.gene58589:CDS |
| ATCATTTGAGACTTCATCCA+TGG | 0.558263 | 5.3:+24808296 | None:intergenic |
| GTCAAAAGAGTGGTGAGAAG+AGG | 0.562252 | 5.3:-24808473 | MS.gene58589:CDS |
| CATGACTAACCTGTGTGACT+AGG | 0.563491 | 5.3:-24808142 | None:intergenic |
| TACGAGGTTCAGAGGAGAAG+AGG | 0.567800 | 5.3:-24808206 | MS.gene58589:CDS |
| CGTTCTCGCAATGATGCTGC+GGG | 0.569360 | 5.3:-24808357 | MS.gene58589:CDS |
| ATGTTTGGAGCAAGTCTCCA+AGG | 0.570714 | 5.3:-24809079 | MS.gene58589:CDS |
| TACACTTGGGCACCATCATG+TGG | 0.573130 | 5.3:-24808645 | MS.gene58589:CDS |
| TCGTTCTCGCAATGATGCTG+CGG | 0.583184 | 5.3:-24808358 | MS.gene58589:CDS |
| TGCATCTATTGGTGAAGTGA+TGG | 0.591393 | 5.3:-24808331 | MS.gene58589:CDS |
| GCTGATGTTTAAGCCTGCAA+GGG | 0.594722 | 5.3:-24808244 | MS.gene58589:CDS |
| TCTTCTCCTCTGAACCTCGT+AGG | 0.595412 | 5.3:+24808208 | None:intergenic |
| GATAGCGAGGATGCAAGTGT+TGG | 0.599986 | 5.3:-24808549 | MS.gene58589:CDS |
| GATGGCAATACCCCACATGA+TGG | 0.601622 | 5.3:+24808633 | None:intergenic |
| CCTCCTCACCTAGTGATGGG+GGG | 0.608278 | 5.3:-24809155 | MS.gene58589:CDS |
| TGAGAAAGGATTGGAGAGCA+TGG | 0.612846 | 5.3:-24808939 | MS.gene58589:CDS |
| GCTACAACAGTGTTGTTCAC+AGG | 0.614063 | 5.3:+24808877 | None:intergenic |
| GTGGTTAACTCATTGGCGAA+TGG | 0.617203 | 5.3:+24808699 | None:intergenic |
| AGAAGGATCTGGCGATAGCG+AGG | 0.624952 | 5.3:-24808562 | MS.gene58589:CDS |
| AACCGGACTGAAGTATGAGA+AGG | 0.626830 | 5.3:-24808989 | MS.gene58589:CDS |
| ACACTTGGGCACCATCATGT+GGG | 0.626839 | 5.3:-24808644 | MS.gene58589:CDS |
| CACTACATCCTTGAAAAGTG+TGG | 0.627204 | 5.3:+24808680 | None:intergenic |
| AAAACTGATGGTCAAAAGAG+TGG | 0.632803 | 5.3:-24808483 | MS.gene58589:CDS |
| TTGTAGCACCTGATGAGTGG+TGG | 0.632872 | 5.3:-24808861 | MS.gene58589:CDS |
| GGTTAGTCATGAATGGCAGT+GGG | 0.638001 | 5.3:+24808154 | None:intergenic |
| CCCATACATCCTAGTCACAC+AGG | 0.638813 | 5.3:+24808133 | None:intergenic |
| GAGAGAAACTGGATTAGGGT+GGG | 0.643865 | 5.3:-24808902 | MS.gene58589:CDS |
| GTCTCCAAGGGAGAACGTGT+TGG | 0.644338 | 5.3:-24809066 | MS.gene58589:CDS |
| CACTTGGGCACCATCATGTG+GGG | 0.646121 | 5.3:-24808643 | MS.gene58589:CDS |
| TGTTTGGAGCAAGTCTCCAA+GGG | 0.647436 | 5.3:-24809078 | MS.gene58589:CDS |
| TGTAGCACCTGATGAGTGGT+GGG | 0.651018 | 5.3:-24808860 | MS.gene58589:CDS |
| GATGGCTGAGCTTCACACCA+TGG | 0.663371 | 5.3:-24808313 | MS.gene58589:CDS |
| GGCTAATGGAGAGTACACTT+GGG | 0.684848 | 5.3:-24808658 | MS.gene58589:CDS |
| CTGTTGTAGCACCTGATGAG+TGG | 0.705267 | 5.3:-24808864 | MS.gene58589:CDS |
| GAGATGTTTATTTCCCTACG+AGG | 0.736446 | 5.3:-24808222 | MS.gene58589:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGAAAAATTTAGGAACAA+GGG | - | chr5.3:24808603-24808622 | MS.gene58589:CDS | 20.0% |
| !! | TTATGAAAAATTTAGGAACA+AGG | - | chr5.3:24808602-24808621 | MS.gene58589:CDS | 20.0% |
| !!! | TCCTAAATTTTTCATAAAGA+GGG | + | chr5.3:24808599-24808618 | None:intergenic | 20.0% |
| !!! | TTCCTAAATTTTTCATAAAG+AGG | + | chr5.3:24808600-24808619 | None:intergenic | 20.0% |
| ! | AATACCAAGAAAAATGTTGT+GGG | - | chr5.3:24808149-24808168 | MS.gene58589:CDS | 25.0% |
| ! | AATTCTTCACAAAAAACTGA+TGG | - | chr5.3:24808834-24808853 | MS.gene58589:intron | 25.0% |
| ! | ACAACTGAATTTGGAAATAT+TGG | - | chr5.3:24808807-24808826 | MS.gene58589:intron | 25.0% |
| ! | ACCCTCTTTATGAAAAATTT+AGG | - | chr5.3:24808595-24808614 | MS.gene58589:CDS | 25.0% |
| ! | ATGAAAAATTTAGGAACAAG+GGG | - | chr5.3:24808604-24808623 | MS.gene58589:CDS | 25.0% |
| ! | CAACTGAATTTGGAAATATT+GGG | - | chr5.3:24808808-24808827 | MS.gene58589:intron | 25.0% |
| AAAAAGGGTGGACAAATATT+AGG | - | chr5.3:24808300-24808319 | MS.gene58589:CDS | 30.0% | |
| AAAAGGGTGGACAAATATTA+GGG | - | chr5.3:24808301-24808320 | MS.gene58589:CDS | 30.0% | |
| AACACGAGTATACATATCTA+AGG | + | chr5.3:24808541-24808560 | None:intergenic | 30.0% | |
| AGAGCATGGTATAACTTATT+TGG | - | chr5.3:24808404-24808423 | MS.gene58589:CDS | 30.0% | |
| GAATACCAAGAAAAATGTTG+TGG | - | chr5.3:24808148-24808167 | MS.gene58589:CDS | 30.0% | |
| GAGCATGGTATAACTTATTT+GGG | - | chr5.3:24808405-24808424 | MS.gene58589:CDS | 30.0% | |
| GCAGAAAACAAAAGCTAATT+GGG | - | chr5.3:24808196-24808215 | MS.gene58589:CDS | 30.0% | |
| GTCCGATTTCATAATTTAAC+CGG | - | chr5.3:24808323-24808342 | MS.gene58589:CDS | 30.0% | |
| GTCCGGTTAAATTATGAAAT+CGG | + | chr5.3:24808328-24808347 | None:intergenic | 30.0% | |
| TGTATTACATACCAGTTGTT+TGG | + | chr5.3:24808498-24808517 | None:intergenic | 30.0% | |
| ! | AAACTTTGTGAAAGCATGTT+TGG | - | chr5.3:24808235-24808254 | MS.gene58589:CDS | 30.0% |
| ! | ATGATCTTTATTTGCATGCT+CGG | - | chr5.3:24809051-24809070 | MS.gene58589:CDS | 30.0% |
| ! | GTACGAGCTTTTCAAAAAAA+GGG | - | chr5.3:24808285-24808304 | MS.gene58589:CDS | 30.0% |
| ! | TCATCATTTTCAAACACAGA+TGG | + | chr5.3:24808717-24808736 | None:intergenic | 30.0% |
| ! | TGATAATGATGATGATGATG+AGG | - | chr5.3:24808734-24808753 | MS.gene58589:CDS | 30.0% |
| AAAACTGATGGTCAAAAGAG+TGG | - | chr5.3:24808846-24808865 | MS.gene58589:CDS | 35.0% | |
| AACTTATTTGGGAGAGAAAC+TGG | - | chr5.3:24808416-24808435 | MS.gene58589:CDS | 35.0% | |
| AGAAGGCTCAACTAAAGAAT+AGG | - | chr5.3:24808357-24808376 | MS.gene58589:CDS | 35.0% | |
| GATGTGAGTTATGAAAATGC+AGG | - | chr5.3:24808569-24808588 | MS.gene58589:CDS | 35.0% | |
| GGCAGAAAACAAAAGCTAAT+TGG | - | chr5.3:24808195-24808214 | MS.gene58589:CDS | 35.0% | |
| ! | ATCATTTGAGACTTCATCCA+TGG | + | chr5.3:24809036-24809055 | None:intergenic | 35.0% |
| ! | GAAAAGTGTGGTTAACTCAT+TGG | + | chr5.3:24808640-24808659 | None:intergenic | 35.0% |
| ! | GGTACGAGCTTTTCAAAAAA+AGG | - | chr5.3:24808284-24808303 | MS.gene58589:CDS | 35.0% |
| ! | TGAGTTAACCACACTTTTCA+AGG | - | chr5.3:24808641-24808660 | MS.gene58589:CDS | 35.0% |
| !!! | GCACTTGCTTTTTTCTTACT+TGG | + | chr5.3:24808906-24808925 | None:intergenic | 35.0% |
| ATGACAGTCTGAGAAAGGAT+TGG | - | chr5.3:24808381-24808400 | MS.gene58589:CDS | 40.0% | |
| CACTACATCCTTGAAAAGTG+TGG | + | chr5.3:24808652-24808671 | None:intergenic | 40.0% | |
| GAAATAAACATCTCCCTTGC+AGG | + | chr5.3:24809101-24809120 | None:intergenic | 40.0% | |
| GAGATGTTTATTTCCCTACG+AGG | - | chr5.3:24809107-24809126 | MS.gene58589:CDS | 40.0% | |
| GTTGGAGCAACAACTGAATT+TGG | - | chr5.3:24808798-24808817 | MS.gene58589:intron | 40.0% | |
| TAGGTATGACAGTCTGAGAA+AGG | - | chr5.3:24808376-24808395 | MS.gene58589:CDS | 40.0% | |
| TGCATCTATTGGTGAAGTGA+TGG | - | chr5.3:24808998-24809017 | MS.gene58589:CDS | 40.0% | |
| TTCAAGGATGTAGTGGCTAA+TGG | - | chr5.3:24808657-24808676 | MS.gene58589:CDS | 40.0% | |
| TTGGGAGAGAAACTGGATTA+GGG | - | chr5.3:24808423-24808442 | MS.gene58589:CDS | 40.0% | |
| TTTGGGAGAGAAACTGGATT+AGG | - | chr5.3:24808422-24808441 | MS.gene58589:CDS | 40.0% | |
| ! | CGAGCTTTTCAAAAAAAGGG+TGG | - | chr5.3:24808288-24808307 | MS.gene58589:CDS | 40.0% |
| ! | GGAACCCACAACATTTTTCT+TGG | + | chr5.3:24808156-24808175 | None:intergenic | 40.0% |
| ! | TCACACAGGTTAGTCATGAA+TGG | + | chr5.3:24809185-24809204 | None:intergenic | 40.0% |
| !! | CACACTTTTCAAGGATGTAG+TGG | - | chr5.3:24808650-24808669 | MS.gene58589:CDS | 40.0% |
| AACCGGACTGAAGTATGAGA+AGG | - | chr5.3:24808340-24808359 | MS.gene58589:CDS | 45.0% | |
| AGCCTTCTCATACTTCAGTC+CGG | + | chr5.3:24808345-24808364 | None:intergenic | 45.0% | |
| CAGAGGAGAAGAGGTTGAAT+TGG | - | chr5.3:24809132-24809151 | MS.gene58589:CDS | 45.0% | |
| GCTGATGTTTAAGCCTGCAA+GGG | - | chr5.3:24809085-24809104 | MS.gene58589:CDS | 45.0% | |
| GTCAAAAGAGTGGTGAGAAG+AGG | - | chr5.3:24808856-24808875 | MS.gene58589:CDS | 45.0% | |
| GTGGTTAACTCATTGGCGAA+TGG | + | chr5.3:24808633-24808652 | None:intergenic | 45.0% | |
| TATTTCCCTACGAGGTTCAG+AGG | - | chr5.3:24809115-24809134 | MS.gene58589:CDS | 45.0% | |
| TGAGAAAGGATTGGAGAGCA+TGG | - | chr5.3:24808390-24808409 | MS.gene58589:CDS | 45.0% | |
| TGCTGATGTTTAAGCCTGCA+AGG | - | chr5.3:24809084-24809103 | MS.gene58589:CDS | 45.0% | |
| ! | AGGTTAGTCATGAATGGCAG+TGG | + | chr5.3:24809179-24809198 | None:intergenic | 45.0% |
| ! | ATGTTTGGAGCAAGTCTCCA+AGG | - | chr5.3:24808250-24808269 | MS.gene58589:CDS | 45.0% |
| ! | GAGAGAAACTGGATTAGGGT+GGG | - | chr5.3:24808427-24808446 | MS.gene58589:CDS | 45.0% |
| ! | GCTACAACAGTGTTGTTCAC+AGG | + | chr5.3:24808455-24808474 | None:intergenic | 45.0% |
| ! | GGCTAATGGAGAGTACACTT+GGG | - | chr5.3:24808671-24808690 | MS.gene58589:CDS | 45.0% |
| ! | GGTTAGTCATGAATGGCAGT+GGG | + | chr5.3:24809178-24809197 | None:intergenic | 45.0% |
| ! | TGATGATGATGATGAGGCAG+TGG | - | chr5.3:24808740-24808759 | MS.gene58589:CDS | 45.0% |
| ! | TGGCTAATGGAGAGTACACT+TGG | - | chr5.3:24808670-24808689 | MS.gene58589:CDS | 45.0% |
| ! | TGTTTGGAGCAAGTCTCCAA+GGG | - | chr5.3:24808251-24808270 | MS.gene58589:CDS | 45.0% |
| ACACTTGGGCACCATCATGT+GGG | - | chr5.3:24808685-24808704 | MS.gene58589:CDS | 50.0% | |
| CTGTTGTAGCACCTGATGAG+TGG | - | chr5.3:24808465-24808484 | MS.gene58589:CDS | 50.0% | |
| CTTCTCCTCTGAACCTCGTA+GGG | + | chr5.3:24809123-24809142 | None:intergenic | 50.0% | |
| GATAGCGAGGATGCAAGTGT+TGG | - | chr5.3:24808780-24808799 | MS.gene58589:intron | 50.0% | |
| GATGGCAATACCCCACATGA+TGG | + | chr5.3:24808699-24808718 | None:intergenic | 50.0% | |
| GCTGCGGGTAATGCATCTAT+TGG | - | chr5.3:24808987-24809006 | MS.gene58589:CDS | 50.0% | |
| GTTCCTCCTCACCTAGTGAT+GGG | - | chr5.3:24808171-24808190 | MS.gene58589:CDS | 50.0% | |
| TACACTTGGGCACCATCATG+TGG | - | chr5.3:24808684-24808703 | MS.gene58589:CDS | 50.0% | |
| TACGAGGTTCAGAGGAGAAG+AGG | - | chr5.3:24809123-24809142 | MS.gene58589:CDS | 50.0% | |
| TCGTTCTCGCAATGATGCTG+CGG | - | chr5.3:24808971-24808990 | MS.gene58589:CDS | 50.0% | |
| TCTTCTCCTCTGAACCTCGT+AGG | + | chr5.3:24809124-24809143 | None:intergenic | 50.0% | |
| TGTAGCACCTGATGAGTGGT+GGG | - | chr5.3:24808469-24808488 | MS.gene58589:CDS | 50.0% | |
| TTCCTCCTCACCTAGTGATG+GGG | - | chr5.3:24808172-24808191 | MS.gene58589:CDS | 50.0% | |
| TTGTAGCACCTGATGAGTGG+TGG | - | chr5.3:24808468-24808487 | MS.gene58589:CDS | 50.0% | |
| ! | GGAGAGAAACTGGATTAGGG+TGG | - | chr5.3:24808426-24808445 | MS.gene58589:CDS | 50.0% |
| AGAAGGATCTGGCGATAGCG+AGG | - | chr5.3:24808767-24808786 | MS.gene58589:intron | 55.0% | |
| CACTTGGGCACCATCATGTG+GGG | - | chr5.3:24808686-24808705 | MS.gene58589:CDS | 55.0% | |
| CGTACCAACACGTTCTCCCT+TGG | + | chr5.3:24808270-24808289 | None:intergenic | 55.0% | |
| CGTTCTCGCAATGATGCTGC+GGG | - | chr5.3:24808972-24808991 | MS.gene58589:CDS | 55.0% | |
| GATGAGGCAGTGGCTGTAGA+AGG | - | chr5.3:24808750-24808769 | MS.gene58589:intron | 55.0% | |
| GATGGCTGAGCTTCACACCA+TGG | - | chr5.3:24809016-24809035 | MS.gene58589:CDS | 55.0% | |
| GCAGTGGCTGTAGAAGGATC+TGG | - | chr5.3:24808756-24808775 | MS.gene58589:intron | 55.0% | |
| GGTTCCTCCTCACCTAGTGA+TGG | - | chr5.3:24808170-24808189 | MS.gene58589:CDS | 55.0% | |
| GTCTCCAAGGGAGAACGTGT+TGG | - | chr5.3:24808263-24808282 | MS.gene58589:CDS | 55.0% | |
| TCCTCCTCACCTAGTGATGG+GGG | - | chr5.3:24808173-24808192 | MS.gene58589:CDS | 55.0% | |
| TTGGTCTCCCACCACTCATC+AGG | + | chr5.3:24808479-24808498 | None:intergenic | 55.0% | |
| ! | GTGGTGGGAGACCAAACAAC+TGG | - | chr5.3:24808484-24808503 | MS.gene58589:CDS | 55.0% |
| ! | GTTTTCTGCCCCCCATCACT+AGG | + | chr5.3:24808185-24808204 | None:intergenic | 55.0% |
| ! | CCTCCTCACCTAGTGATGGG+GGG | - | chr5.3:24808174-24808193 | MS.gene58589:CDS | 60.0% |
| CCCCCCATCACTAGGTGAGG+AGG | + | chr5.3:24808177-24808196 | None:intergenic | 65.0% | |
| CTGCCCCCCATCACTAGGTG+AGG | + | chr5.3:24808180-24808199 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 24808143 | 24809208 | 24808143 | ID=MS.gene58589 |
| chr5.3 | mRNA | 24808143 | 24809208 | 24808143 | ID=MS.gene58589.t1;Parent=MS.gene58589 |
| chr5.3 | exon | 24808846 | 24809208 | 24808846 | ID=MS.gene58589.t1.exon1;Parent=MS.gene58589.t1 |
| chr5.3 | CDS | 24808846 | 24809208 | 24808846 | ID=cds.MS.gene58589.t1;Parent=MS.gene58589.t1 |
| chr5.3 | exon | 24808143 | 24808760 | 24808143 | ID=MS.gene58589.t1.exon2;Parent=MS.gene58589.t1 |
| chr5.3 | CDS | 24808143 | 24808760 | 24808143 | ID=cds.MS.gene58589.t1;Parent=MS.gene58589.t1 |
| Gene Sequence |
| Protein sequence |
