Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59291.t1 | AFK35437.1 | 96.4 | 221 | 8 | 0 | 1 | 221 | 1 | 221 | 4.40E-114 | 420.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59291.t1 | I3S595 | 96.4 | 221 | 8 | 0 | 1 | 221 | 1 | 221 | 3.2e-114 | 420.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene59291 | 0.809903 | 1.50E-50 | -1.69E-46 |
| MS.gene051181 | MS.gene59291 | 0.816266 | 6.04E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene59291 | 0.816545 | 5.23E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene59291 | 0.808777 | 2.62E-50 | -1.69E-46 |
| MS.gene054142 | MS.gene59291 | 0.801207 | 1.00E-48 | -1.69E-46 |
| MS.gene055694 | MS.gene59291 | 0.802791 | 4.75E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene59291 | 0.841926 | 3.53E-58 | -1.69E-46 |
| MS.gene057208 | MS.gene59291 | 0.806166 | 9.39E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene59291 | 0.810045 | 1.40E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59291.t1 | MTR_6g060390 | 95.928 | 221 | 9 | 0 | 1 | 221 | 1 | 221 | 8.62e-158 | 436 |
| MS.gene59291.t1 | MTR_6g060440 | 94.118 | 221 | 11 | 1 | 1 | 221 | 1 | 219 | 9.40e-154 | 425 |
| MS.gene59291.t1 | MTR_1g076800 | 70.925 | 227 | 53 | 6 | 1 | 221 | 1 | 220 | 4.79e-110 | 315 |
| MS.gene59291.t1 | MTR_4g023950 | 48.416 | 221 | 107 | 4 | 1 | 220 | 1 | 215 | 1.39e-60 | 189 |
| MS.gene59291.t1 | MTR_7g117380 | 31.696 | 224 | 135 | 5 | 4 | 213 | 3 | 222 | 1.14e-28 | 107 |
| MS.gene59291.t1 | MTR_4g102220 | 28.889 | 225 | 139 | 6 | 4 | 212 | 5 | 224 | 5.74e-23 | 92.8 |
| MS.gene59291.t1 | MTR_5g011800 | 28.821 | 229 | 138 | 9 | 4 | 212 | 5 | 228 | 1.31e-21 | 89.4 |
| MS.gene59291.t1 | MTR_4g024370 | 30.172 | 232 | 134 | 9 | 4 | 212 | 5 | 231 | 1.03e-20 | 87.0 |
| MS.gene59291.t1 | MTR_4g024370 | 30.172 | 232 | 134 | 9 | 4 | 212 | 5 | 231 | 1.03e-20 | 87.0 |
| MS.gene59291.t1 | MTR_7g117380 | 29.670 | 182 | 115 | 4 | 41 | 213 | 3 | 180 | 2.78e-19 | 82.0 |
| MS.gene59291.t1 | MTR_5g011820 | 28.261 | 230 | 138 | 9 | 4 | 212 | 8 | 231 | 3.38e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59291.t1 | AT5G62280 | 52.991 | 234 | 96 | 6 | 1 | 221 | 1 | 233 | 7.35e-69 | 211 |
| MS.gene59291.t1 | AT2G45360 | 31.532 | 222 | 127 | 8 | 4 | 212 | 5 | 214 | 2.19e-23 | 94.0 |
| MS.gene59291.t1 | AT3G60780 | 30.088 | 226 | 128 | 9 | 4 | 212 | 5 | 217 | 1.60e-21 | 89.0 |
| MS.gene59291.t1 | AT1G12320 | 29.954 | 217 | 136 | 6 | 4 | 213 | 5 | 212 | 1.41e-13 | 67.4 |
| MS.gene59291.t1 | AT1G62840 | 30.396 | 227 | 132 | 8 | 4 | 212 | 5 | 223 | 2.50e-12 | 64.3 |
Find 33 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTCCAGATTCAAATTT+AGG | 0.192509 | 6.4:-44273880 | None:intergenic |
| TGGAAACAATGCACAATTAA+TGG | 0.255453 | 6.4:+44273444 | MS.gene59291:CDS |
| AAAAGGAGCACAAGAATTAT+TGG | 0.277971 | 6.4:+44273630 | MS.gene59291:CDS |
| CCATCAAAACAATGGCTTAC+TGG | 0.279683 | 6.4:+44273030 | None:intergenic |
| ATTGGTTGTTGTAAACAAAA+AGG | 0.293042 | 6.4:+44273727 | MS.gene59291:CDS |
| AAAACTCAATTGCTTCCTAT+TGG | 0.296456 | 6.4:+44273811 | MS.gene59291:CDS |
| GGGTTGTTGGTGACAAGATT+TGG | 0.325248 | 6.4:+44273838 | MS.gene59291:CDS |
| CTATCTCAGCACTATGAAAA+TGG | 0.327097 | 6.4:+44273076 | MS.gene59291:CDS |
| TACAATGCATTTAGTTCTAA+AGG | 0.346376 | 6.4:+44273766 | MS.gene59291:CDS |
| ATTAGTCCTAAATTTGAATC+TGG | 0.359441 | 6.4:+44273874 | MS.gene59291:CDS |
| AATCAAGTTCAATTCATGAT+TGG | 0.360902 | 6.4:+44273607 | MS.gene59291:CDS |
| CAATCTCTTCATGATTCATA+AGG | 0.388987 | 6.4:-44273690 | None:intergenic |
| ACAATGCATTTAGTTCTAAA+GGG | 0.423043 | 6.4:+44273767 | MS.gene59291:CDS |
| GTGATTAGATCTTCGTTAGT+AGG | 0.434432 | 6.4:-44273556 | None:intergenic |
| GTTCAATTCATGATTGGAAA+AGG | 0.436833 | 6.4:+44273613 | MS.gene59291:CDS |
| ACCAACAACCCTTGTCCAAT+AGG | 0.444260 | 6.4:-44273826 | None:intergenic |
| ATTGTTAAATGTGTTCAAAT+TGG | 0.445248 | 6.4:+44273709 | MS.gene59291:CDS |
| TCCTATTGGACAAGGGTTGT+TGG | 0.448779 | 6.4:+44273825 | MS.gene59291:CDS |
| TTCACAATCCAATGACTTCG+AGG | 0.467504 | 6.4:-44273910 | None:intergenic |
| GTAATAGTCCTCGAAGTCAT+TGG | 0.481622 | 6.4:+44273902 | MS.gene59291:CDS |
| CAATTGCTTCCTATTGGACA+AGG | 0.513808 | 6.4:+44273817 | MS.gene59291:CDS |
| TTTATATCAGCACTAGCAGC+TGG | 0.521152 | 6.4:+44273424 | MS.gene59291:CDS |
| CTTGATTAGAAGCTACTCCT+AGG | 0.528611 | 6.4:-44273591 | None:intergenic |
| GCTGCTGCTAATCAAACAAA+TGG | 0.531059 | 6.4:+44273514 | MS.gene59291:CDS |
| GTGAAGGTTGATAAATGTAC+TGG | 0.532119 | 6.4:+44273928 | MS.gene59291:CDS |
| CATTTAGTTCTAAAGGGTCA+TGG | 0.534221 | 6.4:+44273773 | MS.gene59291:CDS |
| GTCCTAAATTTGAATCTGGA+AGG | 0.539804 | 6.4:+44273878 | MS.gene59291:CDS |
| AAAGGGTCATGGAGATCTTG+TGG | 0.543729 | 6.4:+44273784 | MS.gene59291:CDS |
| TTAATGGTTGTAGCTTGTGC+TGG | 0.544219 | 6.4:+44273460 | MS.gene59291:CDS |
| GATATAAATTCAGCTACAGC+AGG | 0.551350 | 6.4:-44273409 | None:intergenic |
| TCGAAGTCATTGGATTGTGA+AGG | 0.587297 | 6.4:+44273912 | MS.gene59291:CDS |
| TATAGAGTTAGACTTCCTCA+AGG | 0.620428 | 6.4:+44273964 | MS.gene59291:CDS |
| AATTGCTTCCTATTGGACAA+GGG | 0.636815 | 6.4:+44273818 | MS.gene59291:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAGATGAAAATTTATAAA+TGG | - | chr6.4:44273325-44273344 | None:intergenic | 15.0% |
| !!! | AAACATTTTGGTTATTAAAT+CGG | - | chr6.4:44273172-44273191 | None:intergenic | 15.0% |
| !!! | ATCTTTTGTTATTTTTCAAT+AGG | + | chr6.4:44273369-44273388 | MS.gene59291:intron | 15.0% |
| !!! | TCTTTTGTTATTTTTCAATA+GGG | + | chr6.4:44273370-44273389 | MS.gene59291:intron | 15.0% |
| !! | ATTGTTAAATGTGTTCAAAT+TGG | + | chr6.4:44273709-44273728 | MS.gene59291:CDS | 20.0% |
| !!! | TATCGAATTTTAAATAGTCA+AGG | - | chr6.4:44273141-44273160 | None:intergenic | 20.0% |
| ! | AATCAAGTTCAATTCATGAT+TGG | + | chr6.4:44273607-44273626 | MS.gene59291:CDS | 25.0% |
| ! | ATTAGTCCTAAATTTGAATC+TGG | + | chr6.4:44273874-44273893 | MS.gene59291:CDS | 25.0% |
| ! | ATTGGTTGTTGTAAACAAAA+AGG | + | chr6.4:44273727-44273746 | MS.gene59291:CDS | 25.0% |
| ! | TAACATTAACAACTAATGGT+TGG | - | chr6.4:44273246-44273265 | None:intergenic | 25.0% |
| ! | TCGTTAACATTAACAACTAA+TGG | - | chr6.4:44273250-44273269 | None:intergenic | 25.0% |
| !!! | ACAATGCATTTAGTTCTAAA+GGG | + | chr6.4:44273767-44273786 | MS.gene59291:CDS | 25.0% |
| !!! | TACAATGCATTTAGTTCTAA+AGG | + | chr6.4:44273766-44273785 | MS.gene59291:CDS | 25.0% |
| !!! | TATTTTTCAATAGGGTCAAA+AGG | + | chr6.4:44273378-44273397 | MS.gene59291:intron | 25.0% |
| AAAACTCAATTGCTTCCTAT+TGG | + | chr6.4:44273811-44273830 | MS.gene59291:CDS | 30.0% | |
| CAATCTCTTCATGATTCATA+AGG | - | chr6.4:44273693-44273712 | None:intergenic | 30.0% | |
| GTTCAATTCATGATTGGAAA+AGG | + | chr6.4:44273613-44273632 | MS.gene59291:CDS | 30.0% | |
| TCGATTTAGCAATTTAGAGA+GGG | + | chr6.4:44273205-44273224 | MS.gene59291:intron | 30.0% | |
| TGGAAACAATGCACAATTAA+TGG | + | chr6.4:44273444-44273463 | MS.gene59291:CDS | 30.0% | |
| ! | AAAAGGAGCACAAGAATTAT+TGG | + | chr6.4:44273630-44273649 | MS.gene59291:CDS | 30.0% |
| ! | ATCGATTTAGCAATTTAGAG+AGG | + | chr6.4:44273204-44273223 | MS.gene59291:intron | 30.0% |
| AATTGCTTCCTATTGGACAA+GGG | + | chr6.4:44273818-44273837 | MS.gene59291:CDS | 35.0% | |
| ATCACGTCGAAAAAAATCCT+AGG | + | chr6.4:44273574-44273593 | MS.gene59291:CDS | 35.0% | |
| CTATCTCAGCACTATGAAAA+TGG | + | chr6.4:44273076-44273095 | MS.gene59291:CDS | 35.0% | |
| CTCCTTCCAGATTCAAATTT+AGG | - | chr6.4:44273883-44273902 | None:intergenic | 35.0% | |
| CTCTAACTCAATCGCTAAAT+CGG | + | chr6.4:44273288-44273307 | MS.gene59291:intron | 35.0% | |
| GATATAAATTCAGCTACAGC+AGG | - | chr6.4:44273412-44273431 | None:intergenic | 35.0% | |
| GTCCTAAATTTGAATCTGGA+AGG | + | chr6.4:44273878-44273897 | MS.gene59291:CDS | 35.0% | |
| GTGATTAGATCTTCGTTAGT+AGG | - | chr6.4:44273559-44273578 | None:intergenic | 35.0% | |
| TATAGAGTTAGACTTCCTCA+AGG | + | chr6.4:44273964-44273983 | MS.gene59291:CDS | 35.0% | |
| ! | CAAAAAGGTACAGTTGTTGT+TGG | + | chr6.4:44273742-44273761 | MS.gene59291:CDS | 35.0% |
| ! | CACTAGACACTGAAACATTT+TGG | - | chr6.4:44273184-44273203 | None:intergenic | 35.0% |
| !! | CATTTAGTTCTAAAGGGTCA+TGG | + | chr6.4:44273773-44273792 | MS.gene59291:CDS | 35.0% |
| !! | CATTTTCATAGTGCTGAGAT+AGG | - | chr6.4:44273078-44273097 | None:intergenic | 35.0% |
| !! | GTGAAGGTTGATAAATGTAC+TGG | + | chr6.4:44273928-44273947 | MS.gene59291:CDS | 35.0% |
| CAATTGCTTCCTATTGGACA+AGG | + | chr6.4:44273817-44273836 | MS.gene59291:CDS | 40.0% | |
| CTTGATTAGAAGCTACTCCT+AGG | - | chr6.4:44273594-44273613 | None:intergenic | 40.0% | |
| GCTGCTGCTAATCAAACAAA+TGG | + | chr6.4:44273514-44273533 | MS.gene59291:CDS | 40.0% | |
| GTAATAGTCCTCGAAGTCAT+TGG | + | chr6.4:44273902-44273921 | MS.gene59291:CDS | 40.0% | |
| TCGAAGTCATTGGATTGTGA+AGG | + | chr6.4:44273912-44273931 | MS.gene59291:CDS | 40.0% | |
| TTCACAATCCAATGACTTCG+AGG | - | chr6.4:44273913-44273932 | None:intergenic | 40.0% | |
| TTTATATCAGCACTAGCAGC+TGG | + | chr6.4:44273424-44273443 | MS.gene59291:CDS | 40.0% | |
| ! | TTAATGGTTGTAGCTTGTGC+TGG | + | chr6.4:44273460-44273479 | MS.gene59291:CDS | 40.0% |
| AAAGGGTCATGGAGATCTTG+TGG | + | chr6.4:44273784-44273803 | MS.gene59291:CDS | 45.0% | |
| ACCAACAACCCTTGTCCAAT+AGG | - | chr6.4:44273829-44273848 | None:intergenic | 45.0% | |
| TCCTATTGGACAAGGGTTGT+TGG | + | chr6.4:44273825-44273844 | MS.gene59291:CDS | 45.0% | |
| !! | AGTGCTGAGATAGGCTTTTG+TGG | - | chr6.4:44273069-44273088 | None:intergenic | 45.0% |
| !! | GGGTTGTTGGTGACAAGATT+TGG | + | chr6.4:44273838-44273857 | MS.gene59291:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 44273041 | 44273999 | 44273041 | ID=MS.gene59291 |
| chr6.4 | mRNA | 44273041 | 44273999 | 44273041 | ID=MS.gene59291.t1;Parent=MS.gene59291 |
| chr6.4 | exon | 44273041 | 44273097 | 44273041 | ID=MS.gene59291.t1.exon1;Parent=MS.gene59291.t1 |
| chr6.4 | CDS | 44273041 | 44273097 | 44273041 | ID=cds.MS.gene59291.t1;Parent=MS.gene59291.t1 |
| chr6.4 | exon | 44273391 | 44273999 | 44273391 | ID=MS.gene59291.t1.exon2;Parent=MS.gene59291.t1 |
| chr6.4 | CDS | 44273391 | 44273999 | 44273391 | ID=cds.MS.gene59291.t1;Parent=MS.gene59291.t1 |
| Gene Sequence |
| Protein sequence |
