Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59736.t1 | XP_003612111.1 | 100 | 80 | 0 | 0 | 1 | 80 | 154 | 233 | 3.10E-33 | 150.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59736.t1 | G7KGC7 | 100.0 | 80 | 0 | 0 | 1 | 80 | 154 | 233 | 2.2e-33 | 150.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene59736 | 0.814118 | 1.81E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene59736 | 0.810786 | 9.69E-51 | -1.69E-46 |
| MS.gene050114 | MS.gene59736 | 0.813928 | 2.00E-51 | -1.69E-46 |
| MS.gene050317 | MS.gene59736 | 0.8221 | 2.84E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene59736 | 0.805727 | 1.16E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene59736 | 0.818444 | 1.95E-52 | -1.69E-46 |
| MS.gene053381 | MS.gene59736 | 0.815431 | 9.28E-52 | -1.69E-46 |
| MS.gene053786 | MS.gene59736 | 0.803142 | 4.01E-49 | -1.69E-46 |
| MS.gene058654 | MS.gene59736 | 0.819793 | 9.64E-53 | -1.69E-46 |
| MS.gene059360 | MS.gene59736 | 0.821352 | 4.23E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene59736 | 0.827433 | 1.57E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene59736 | 0.832041 | 1.19E-55 | -1.69E-46 |
| MS.gene060869 | MS.gene59736 | 0.820122 | 8.11E-53 | -1.69E-46 |
| MS.gene060870 | MS.gene59736 | 0.806731 | 7.13E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGGAAATCCATGCTTTA+AGG | 0.122064 | 5.4:+11585076 | MS.gene59736:intron |
| GTACTTTATCACATCATATT+TGG | 0.179302 | 5.4:-11585115 | None:intergenic |
| CTCTATCAAGTTTGTTAGTC+AGG | 0.294634 | 5.4:-11584946 | None:intergenic |
| ACTGTAAGTGCAGGAGGATA+AGG | 0.391252 | 5.4:+11584888 | MS.gene59736:intron |
| CCATGCTTTAAGGGCTCAGC+TGG | 0.418734 | 5.4:+11585086 | MS.gene59736:CDS |
| TCAGGAAATCCATGCTTTAA+GGG | 0.423038 | 5.4:+11585077 | MS.gene59736:intron |
| GTTAGTTTCAGCATTCTGAT+TGG | 0.457445 | 5.4:-11584921 | None:intergenic |
| CTGTAAGTGCAGGAGGATAA+GGG | 0.466625 | 5.4:+11584889 | MS.gene59736:intron |
| GTTGGATTTAAACCTTGAAA+GGG | 0.496533 | 5.4:+11584769 | MS.gene59736:CDS |
| GGTTGGATTTAAACCTTGAA+AGG | 0.499726 | 5.4:+11584768 | MS.gene59736:CDS |
| CTTGTTTCAATATCTGCTGT+TGG | 0.511375 | 5.4:+11585150 | MS.gene59736:CDS |
| GAGATAGATAAAGTCACGGC+TGG | 0.523910 | 5.4:+11584740 | MS.gene59736:CDS |
| CCAGCTGAGCCCTTAAAGCA+TGG | 0.549664 | 5.4:-11585086 | None:intergenic |
| AGTCACGGCTGGACAGCGGT+TGG | 0.560017 | 5.4:+11584751 | MS.gene59736:CDS |
| TTACATCAATATACGAAGAA+CGG | 0.580904 | 5.4:-11585178 | None:intergenic |
| TTGGATTTAAACCTTGAAAG+GGG | 0.608817 | 5.4:+11584770 | MS.gene59736:CDS |
| GATGTGATAAAGTACTGCAT+AGG | 0.611784 | 5.4:+11585123 | MS.gene59736:CDS |
| TGGATTTAAACCTTGAAAGG+GGG | 0.618514 | 5.4:+11584771 | MS.gene59736:CDS |
| ATAAAGTCACGGCTGGACAG+CGG | 0.681023 | 5.4:+11584747 | MS.gene59736:CDS |
| GTATGAGATAGATAAAGTCA+CGG | 0.685262 | 5.4:+11584736 | MS.gene59736:CDS |
| TCAATATACGAAGAACGGCG+AGG | 0.726236 | 5.4:-11585173 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATATTAATACAATGTTC+AGG | + | chr5.4:11585059-11585078 | MS.gene59736:intron | 15.0% |
| !! | TAAAGAAAATCAAAATGACT+AGG | - | chr5.4:11585042-11585061 | None:intergenic | 20.0% |
| !! | TCAAACATTAACAATCATAA+TGG | - | chr5.4:11584869-11584888 | None:intergenic | 20.0% |
| ! | GTACTTTATCACATCATATT+TGG | - | chr5.4:11585118-11585137 | None:intergenic | 25.0% |
| ! | TTACATCAATATACGAAGAA+CGG | - | chr5.4:11585181-11585200 | None:intergenic | 25.0% |
| GTATGAGATAGATAAAGTCA+CGG | + | chr5.4:11584736-11584755 | MS.gene59736:CDS | 30.0% | |
| GTTGGATTTAAACCTTGAAA+GGG | + | chr5.4:11584769-11584788 | MS.gene59736:CDS | 30.0% | |
| TTGGATTTAAACCTTGAAAG+GGG | + | chr5.4:11584770-11584789 | MS.gene59736:CDS | 30.0% | |
| CTTGTTTCAATATCTGCTGT+TGG | + | chr5.4:11585150-11585169 | MS.gene59736:CDS | 35.0% | |
| GGTTGGATTTAAACCTTGAA+AGG | + | chr5.4:11584768-11584787 | MS.gene59736:CDS | 35.0% | |
| TAATGTTTGACTGTAAGTGC+AGG | + | chr5.4:11584879-11584898 | MS.gene59736:intron | 35.0% | |
| TCAGGAAATCCATGCTTTAA+GGG | + | chr5.4:11585077-11585096 | MS.gene59736:intron | 35.0% | |
| TGGATTTAAACCTTGAAAGG+GGG | + | chr5.4:11584771-11584790 | MS.gene59736:CDS | 35.0% | |
| TTCAGGAAATCCATGCTTTA+AGG | + | chr5.4:11585076-11585095 | MS.gene59736:intron | 35.0% | |
| TTCAGTAATGCTCAGACAAT+TGG | - | chr5.4:11584817-11584836 | None:intergenic | 35.0% | |
| ! | GTTAGTTTCAGCATTCTGAT+TGG | - | chr5.4:11584924-11584943 | None:intergenic | 35.0% |
| !! | CTCTATCAAGTTTGTTAGTC+AGG | - | chr5.4:11584949-11584968 | None:intergenic | 35.0% |
| !! | GATGTGATAAAGTACTGCAT+AGG | + | chr5.4:11585123-11585142 | MS.gene59736:CDS | 35.0% |
| GTAATTCTTACCCCCTTTCA+AGG | - | chr5.4:11584784-11584803 | None:intergenic | 40.0% | |
| ACTGTAAGTGCAGGAGGATA+AGG | + | chr5.4:11584888-11584907 | MS.gene59736:intron | 45.0% | |
| CTGTAAGTGCAGGAGGATAA+GGG | + | chr5.4:11584889-11584908 | MS.gene59736:intron | 45.0% | |
| GAGATAGATAAAGTCACGGC+TGG | + | chr5.4:11584740-11584759 | MS.gene59736:CDS | 45.0% | |
| TCAATATACGAAGAACGGCG+AGG | - | chr5.4:11585176-11585195 | None:intergenic | 45.0% | |
| TGTTTGACTGTAAGTGCAGG+AGG | + | chr5.4:11584882-11584901 | MS.gene59736:intron | 45.0% | |
| ATAAAGTCACGGCTGGACAG+CGG | + | chr5.4:11584747-11584766 | MS.gene59736:CDS | 50.0% | |
| CCAGCTGAGCCCTTAAAGCA+TGG | - | chr5.4:11585089-11585108 | None:intergenic | 55.0% | |
| !! | CCATGCTTTAAGGGCTCAGC+TGG | + | chr5.4:11585086-11585105 | MS.gene59736:CDS | 55.0% |
| AGTCACGGCTGGACAGCGGT+TGG | + | chr5.4:11584751-11584770 | MS.gene59736:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 11584736 | 11585200 | 11584736 | ID=MS.gene59736 |
| chr5.4 | mRNA | 11584736 | 11585200 | 11584736 | ID=MS.gene59736.t1;Parent=MS.gene59736 |
| chr5.4 | exon | 11584736 | 11584792 | 11584736 | ID=MS.gene59736.t1.exon1;Parent=MS.gene59736.t1 |
| chr5.4 | CDS | 11584736 | 11584792 | 11584736 | ID=cds.MS.gene59736.t1;Parent=MS.gene59736.t1 |
| chr5.4 | exon | 11584901 | 11584967 | 11584901 | ID=MS.gene59736.t1.exon2;Parent=MS.gene59736.t1 |
| chr5.4 | CDS | 11584901 | 11584967 | 11584901 | ID=cds.MS.gene59736.t1;Parent=MS.gene59736.t1 |
| chr5.4 | exon | 11585081 | 11585200 | 11585081 | ID=MS.gene59736.t1.exon3;Parent=MS.gene59736.t1 |
| chr5.4 | CDS | 11585081 | 11585200 | 11585081 | ID=cds.MS.gene59736.t1;Parent=MS.gene59736.t1 |
| Gene Sequence |
| Protein sequence |
