AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59765

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59765.t1 MTR_8g042910 93.927 494 25 2 27 515 123 616 0.0 960
MS.gene59765.t1 MTR_3g010770 78.038 469 100 2 46 511 107 575 0.0 769
MS.gene59765.t1 MTR_7g108875 49.709 515 226 10 6 511 74 564 2.44e-166 483
MS.gene59765.t1 MTR_7g108900 53.571 476 198 9 46 511 98 560 1.07e-165 481
MS.gene59765.t1 MTR_1g054880 52.239 469 212 4 46 511 99 558 2.05e-164 478
MS.gene59765.t1 MTR_7g108890 51.102 499 217 8 28 511 76 562 1.78e-163 475
MS.gene59765.t1 MTR_1g088020 50.104 483 219 7 39 511 85 555 2.50e-158 462
MS.gene59765.t1 MTR_1g088225 49.689 483 219 8 39 511 85 553 3.98e-154 451
MS.gene59765.t1 MTR_1g083520 46.813 502 216 6 16 512 74 529 1.66e-153 449
MS.gene59765.t1 MTR_7g108905 48.164 463 213 7 46 507 116 552 1.28e-145 429
MS.gene59765.t1 MTR_4g124520 48.724 431 186 5 83 511 124 521 3.49e-138 409
MS.gene59765.t1 MTR_2g044810 45.053 475 219 6 37 511 102 534 2.67e-134 400
MS.gene59765.t1 MTR_2g044880 46.237 465 206 10 55 511 97 525 1.12e-132 395
MS.gene59765.t1 MTR_1g008140 44.606 482 227 8 46 511 150 607 2.83e-131 394
MS.gene59765.t1 MTR_0458s0030 64.773 264 93 0 248 511 51 314 6.38e-129 378
MS.gene59765.t1 MTR_1g088040 64.773 264 93 0 248 511 51 314 6.38e-129 378
MS.gene59765.t1 MTR_4g025690 50.694 432 166 10 83 511 126 513 2.84e-128 384
MS.gene59765.t1 MTR_8g104655 44.776 469 216 8 56 511 103 541 9.13e-128 383
MS.gene59765.t1 MTR_4g087980 43.949 471 222 9 45 513 88 518 6.85e-126 377
MS.gene59765.t1 MTR_3g091380 44.792 480 222 9 46 511 147 597 8.89e-126 380
MS.gene59765.t1 MTR_4g025730 46.414 474 196 12 40 511 92 509 7.98e-125 375
MS.gene59765.t1 MTR_8g104645 51.662 331 157 2 182 511 44 372 4.17e-124 368
MS.gene59765.t1 MTR_8g042900 43.991 466 227 8 46 511 150 581 2.11e-122 371
MS.gene59765.t1 MTR_7g112580 43.469 467 230 8 46 511 109 542 4.78e-122 369
MS.gene59765.t1 MTR_4g087970 42.405 474 225 8 45 511 85 517 1.70e-120 364
MS.gene59765.t1 MTR_4g025670 43.617 470 219 8 50 511 58 489 1.15e-118 358
MS.gene59765.t1 MTR_7g050870 43.789 475 216 9 45 511 123 554 1.73e-117 357
MS.gene59765.t1 MTR_4g035980 42.917 480 224 12 40 511 102 539 3.26e-117 356
MS.gene59765.t1 MTR_3g010780 42.257 452 230 8 58 507 148 570 2.00e-116 355
MS.gene59765.t1 MTR_3g008640 41.561 474 233 8 40 511 96 527 3.37e-116 353
MS.gene59765.t1 MTR_3g008720 41.350 474 234 8 40 511 96 527 3.72e-116 353
MS.gene59765.t1 MTR_7g053360 51.351 333 155 4 181 511 200 527 1.77e-115 351
MS.gene59765.t1 MTR_8g023310 41.566 498 261 9 19 511 117 589 2.88e-115 353
MS.gene59765.t1 MTR_3g033690 51.051 333 156 4 181 511 199 526 5.22e-115 350
MS.gene59765.t1 MTR_2g044810 61.111 252 98 0 260 511 214 465 3.84e-114 345
MS.gene59765.t1 MTR_3g008700 41.139 474 235 9 40 511 96 527 4.49e-114 347
MS.gene59765.t1 MTR_7g050950 40.710 479 224 8 45 511 123 553 1.11e-113 347
MS.gene59765.t1 MTR_7g050980 42.526 475 222 10 45 511 124 555 1.97e-112 344
MS.gene59765.t1 MTR_1g083510 38.008 492 278 10 30 510 107 582 2.45e-109 337
MS.gene59765.t1 MTR_7g112590 36.874 499 271 10 30 510 110 582 6.22e-109 337
MS.gene59765.t1 MTR_4g124550 38.604 487 250 7 40 512 143 594 7.42e-106 328
MS.gene59765.t1 MTR_2g044660 50.637 314 148 3 199 512 327 633 5.02e-101 317
MS.gene59765.t1 MTR_1g086190 37.578 479 278 7 35 511 104 563 5.90e-101 315
MS.gene59765.t1 MTR_1g086210 37.864 515 241 13 33 512 100 570 3.27e-99 311
MS.gene59765.t1 MTR_0618s0020 59.912 227 90 1 286 511 2 228 4.55e-98 296
MS.gene59765.t1 MTR_1g083490 34.082 490 279 6 29 507 115 571 2.42e-96 303
MS.gene59765.t1 MTR_1g083470 35.062 482 283 9 30 507 111 566 2.63e-96 303
MS.gene59765.t1 MTR_1g083500 35.062 482 283 9 30 507 111 566 2.63e-96 303
MS.gene59765.t1 MTR_8g104580 39.262 461 220 10 56 511 95 500 1.57e-95 299
MS.gene59765.t1 MTR_4g005170 34.888 493 271 9 27 507 117 571 1.63e-95 303
MS.gene59765.t1 MTR_8g023150 40.716 447 204 11 80 507 174 578 9.20e-95 299
MS.gene59765.t1 MTR_4g005180 44.377 329 176 4 183 507 228 553 4.68e-94 297
MS.gene59765.t1 MTR_8g093460 47.910 311 150 5 209 510 243 550 2.29e-93 295
MS.gene59765.t1 MTR_0458s0010 38.302 483 239 11 39 511 85 518 2.95e-93 293
MS.gene59765.t1 MTR_7g110560 34.725 527 286 11 13 511 53 549 1.92e-92 293
MS.gene59765.t1 MTR_3g095920 46.580 307 151 5 204 500 250 553 6.59e-90 286
MS.gene59765.t1 MTR_5g083620 40.510 353 196 6 165 512 14 357 9.26e-84 264
MS.gene59765.t1 MTR_2g436040 32.848 481 280 8 37 508 97 543 2.83e-83 270
MS.gene59765.t1 MTR_4g120290 32.515 489 275 8 41 510 119 571 1.15e-82 268
MS.gene59765.t1 MTR_7g110440 34.722 504 265 11 37 513 102 568 3.87e-82 266
MS.gene59765.t1 MTR_7g110490 35.510 490 284 11 37 515 102 570 1.45e-80 262
MS.gene59765.t1 MTR_7g110510 35.510 490 284 11 37 515 102 570 1.45e-80 262
MS.gene59765.t1 MTR_4g120280 32.106 489 277 8 41 510 119 571 5.74e-80 261
MS.gene59765.t1 MTR_8g088610 41.358 324 182 5 190 508 53 373 7.95e-78 249
MS.gene59765.t1 MTR_2g436050 32.454 493 299 10 27 510 96 563 7.66e-77 252
MS.gene59765.t1 MTR_7g107180 43.354 316 163 6 198 511 6 307 1.27e-74 238
MS.gene59765.t1 MTR_2g105560 41.587 315 166 6 199 511 41 339 1.40e-73 237
MS.gene59765.t1 MTR_7g110420 32.041 490 301 12 41 513 106 580 8.37e-68 229
MS.gene59765.t1 MTR_7g111560 30.402 523 290 14 3 511 94 556 4.99e-62 213
MS.gene59765.t1 MTR_7g110430 30.140 501 258 11 37 513 102 534 2.04e-61 211
MS.gene59765.t1 MTR_8g102380 32.216 388 219 12 129 500 18 377 1.20e-51 180
MS.gene59765.t1 MTR_1g086540 34.483 348 195 10 164 500 19 344 2.65e-51 178
MS.gene59765.t1 MTR_6g088810 37.705 305 160 8 208 500 91 377 6.36e-51 178
MS.gene59765.t1 MTR_6g012800 36.237 287 157 5 199 474 6 277 1.05e-48 171
MS.gene59765.t1 MTR_7g092780 34.603 315 180 7 197 500 88 387 3.33e-47 169
MS.gene59765.t1 MTR_7g102600 31.215 362 222 7 151 500 47 393 2.46e-46 167
MS.gene59765.t1 MTR_2g039530 36.585 287 155 8 199 478 52 318 4.93e-46 164
MS.gene59765.t1 MTR_6g024165 33.841 328 181 10 185 500 31 334 5.63e-46 164
MS.gene59765.t1 MTR_6g042660 33.557 298 169 8 183 474 22 296 2.05e-45 162
MS.gene59765.t1 MTR_5g083590 32.372 312 158 5 201 511 9 268 3.44e-43 154
MS.gene59765.t1 MTR_8g102380 32.036 334 185 11 129 446 18 325 4.55e-43 156
MS.gene59765.t1 MTR_7g011530 33.451 284 165 4 201 474 8 277 1.49e-42 154
MS.gene59765.t1 MTR_5g095490 33.766 308 175 7 205 500 67 357 1.24e-40 150
MS.gene59765.t1 MTR_1g033050 32.234 273 162 5 209 474 28 284 5.12e-39 144
MS.gene59765.t1 MTR_2g033970 29.316 307 194 6 201 501 58 347 8.36e-38 142
MS.gene59765.t1 MTR_5g012600 30.061 326 199 7 192 505 56 364 1.24e-36 139
MS.gene59765.t1 MTR_5g093970 33.582 268 157 6 214 475 54 306 1.49e-35 135
MS.gene59765.t1 MTR_6g042690 30.986 284 169 6 199 474 39 303 1.72e-35 135
MS.gene59765.t1 MTR_4g124740 27.778 306 185 9 205 500 60 339 5.83e-34 131
MS.gene59765.t1 MTR_8g036225 31.228 285 168 7 199 474 33 298 7.17e-32 125
MS.gene59765.t1 MTR_5g088300 30.686 277 170 5 199 474 37 292 7.91e-32 125
MS.gene59765.t1 MTR_4g051810 31.095 283 175 4 192 474 30 292 8.15e-32 125
MS.gene59765.t1 MTR_2g436600 28.065 310 185 10 201 501 57 337 1.16e-31 125
MS.gene59765.t1 MTR_5g088290 31.179 263 162 5 214 474 59 304 5.49e-30 120
MS.gene59765.t1 MTR_5g088280 30.249 281 176 5 195 475 35 295 1.05e-28 116
MS.gene59765.t1 MTR_2g073390 25.828 302 201 7 205 500 77 361 4.90e-23 100
MS.gene59765.t1 MTR_4g074290 25.413 303 201 8 205 500 77 361 1.21e-22 99.8
MS.gene59765.t1 MTR_0009s0350 25.166 302 203 7 205 500 77 361 9.58e-22 97.4
MS.gene59765.t1 MTR_0009s0340 25.166 302 203 7 205 500 77 361 9.58e-22 97.4
MS.gene59765.t1 MTR_6g025590 31.658 199 115 8 174 362 30 217 2.04e-19 87.8
MS.gene59765.t1 MTR_0408s0030 37.113 97 52 1 378 474 36 123 3.88e-17 79.0
MS.gene59765.t1 MTR_4g130790 30.114 176 111 4 216 383 39 210 1.47e-16 80.1
MS.gene59765.t1 MTR_6g024200 30.814 172 98 5 330 500 125 276 1.23e-15 77.8
MS.gene59765.t1 MTR_7g102580 43.023 86 39 2 276 356 73 153 7.21e-14 69.7
MS.gene59765.t1 MTR_7g102570 30.328 122 73 3 361 480 3 114 6.12e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59765.t1 AT5G04970 62.553 470 166 5 46 511 157 620 0.0 607
MS.gene59765.t1 AT3G10720 60.327 489 183 6 28 511 133 615 0.0 605
MS.gene59765.t1 AT4G02330 50.734 477 220 5 46 511 99 571 1.81e-165 481
MS.gene59765.t1 AT1G02810 50.816 490 210 8 46 511 95 577 4.75e-162 472
MS.gene59765.t1 AT2G47550 49.786 468 222 5 46 511 102 558 7.70e-152 446
MS.gene59765.t1 AT3G10720 74.903 259 65 0 253 511 1 259 4.85e-150 430
MS.gene59765.t1 AT4G33220 44.603 491 248 8 30 513 52 525 9.57e-135 400
MS.gene59765.t1 AT3G43270 44.856 486 240 7 30 511 64 525 4.57e-132 394
MS.gene59765.t1 AT4G33220 48.804 418 195 7 98 513 4 404 5.01e-130 384
MS.gene59765.t1 AT3G60730 45.312 448 218 6 74 511 89 519 2.00e-129 387
MS.gene59765.t1 AT3G05620 46.620 429 191 5 83 511 151 541 1.77e-124 375
MS.gene59765.t1 AT2G26440 44.093 474 236 8 40 511 101 547 2.38e-124 375
MS.gene59765.t1 AT2G45220 58.360 317 122 5 196 511 204 511 8.16e-124 372
MS.gene59765.t1 AT3G49220 46.259 441 204 9 81 513 183 598 1.31e-119 364
MS.gene59765.t1 AT3G49220 46.259 441 204 9 81 513 228 643 3.93e-119 364
MS.gene59765.t1 AT1G11580 42.004 469 233 6 46 511 125 557 8.25e-119 361
MS.gene59765.t1 AT1G11580 42.004 469 233 6 46 511 146 578 1.17e-118 361
MS.gene59765.t1 AT5G53370 43.515 478 233 11 41 511 138 585 1.74e-118 361
MS.gene59765.t1 AT5G53370 43.515 478 233 11 41 511 174 621 4.70e-118 361
MS.gene59765.t1 AT2G43050 44.444 459 210 9 56 511 100 516 6.53e-116 352
MS.gene59765.t1 AT1G23200 52.469 324 143 3 189 511 241 554 4.69e-115 351
MS.gene59765.t1 AT4G00190 53.698 311 139 4 199 508 165 471 1.69e-114 347
MS.gene59765.t1 AT4G02320 42.038 471 226 8 56 511 80 518 1.79e-114 348
MS.gene59765.t1 AT1G53830 42.857 490 259 9 28 511 113 587 5.65e-114 349
MS.gene59765.t1 AT3G10710 42.373 472 225 10 44 511 133 561 7.53e-114 348
MS.gene59765.t1 AT5G04960 43.137 459 221 10 55 507 136 560 2.16e-113 347
MS.gene59765.t1 AT5G51490 43.908 435 203 9 83 511 137 536 7.79e-113 345
MS.gene59765.t1 AT3G14310 53.416 322 146 2 191 511 274 592 4.39e-112 345
MS.gene59765.t1 AT4G02300 41.772 474 231 8 46 511 96 532 3.56e-109 335
MS.gene59765.t1 AT5G51500 49.851 335 160 4 180 511 211 540 8.62e-108 332
MS.gene59765.t1 AT5G27870 37.552 482 260 5 41 515 119 566 8.29e-107 335
MS.gene59765.t1 AT4G03930 51.274 314 147 3 199 511 216 524 5.69e-106 327
MS.gene59765.t1 AT2G26450 38.235 476 249 11 51 512 168 612 1.04e-105 329
MS.gene59765.t1 AT3G59010 40.816 490 243 10 25 511 83 528 1.41e-105 326
MS.gene59765.t1 AT1G53840 37.205 508 274 9 10 505 107 581 4.96e-105 326
MS.gene59765.t1 AT3G27980 50.637 314 149 3 199 511 189 497 6.58e-105 323
MS.gene59765.t1 AT1G11590 50.637 314 149 3 199 511 216 524 7.83e-105 324
MS.gene59765.t1 AT3G05610 37.029 478 262 5 45 515 124 569 1.57e-104 327
MS.gene59765.t1 AT3G47400 41.213 478 241 10 40 511 151 594 5.64e-103 321
MS.gene59765.t1 AT5G49180 48.408 314 158 2 195 507 256 566 5.25e-101 315
MS.gene59765.t1 AT4G33230 38.398 487 276 8 27 512 144 607 8.24e-101 316
MS.gene59765.t1 AT5G20860 36.944 517 269 11 5 511 69 538 1.21e-100 313
MS.gene59765.t1 AT3G06830 37.149 463 272 5 47 508 121 565 7.58e-95 299
MS.gene59765.t1 AT3G14300 39.570 465 237 13 47 501 529 959 2.14e-94 308
MS.gene59765.t1 AT4G15980 40.584 377 216 4 135 507 326 698 8.06e-88 285
MS.gene59765.t1 AT4G15980 40.584 377 216 4 135 507 336 708 1.69e-87 284
MS.gene59765.t1 AT5G09760 45.397 315 157 6 195 499 237 546 6.83e-85 273
MS.gene59765.t1 AT5G09760 45.397 315 157 6 195 499 263 572 1.06e-84 273
MS.gene59765.t1 AT5G64640 43.614 321 165 6 195 503 285 601 1.37e-82 268
MS.gene59765.t1 AT1G11370 48.988 247 123 1 196 442 43 286 3.41e-81 254
MS.gene59765.t1 AT5G20860 32.998 497 248 10 5 491 69 490 4.70e-78 254
MS.gene59765.t1 AT2G47040 41.641 329 185 4 186 511 271 595 6.56e-74 245
MS.gene59765.t1 AT2G47030 41.587 315 177 4 200 511 278 588 2.41e-73 243
MS.gene59765.t1 AT3G62170 42.857 315 173 4 200 511 278 588 2.68e-73 243
MS.gene59765.t1 AT2G36710 33.758 314 187 5 199 505 91 390 2.24e-51 180
MS.gene59765.t1 AT5G19730 31.429 385 220 10 131 500 26 381 1.11e-50 178
MS.gene59765.t1 AT1G05310 33.851 322 183 6 197 505 89 393 4.17e-48 171
MS.gene59765.t1 AT3G17060 31.392 309 189 6 199 501 46 337 3.64e-46 165
MS.gene59765.t1 AT2G21610 32.450 302 184 6 199 500 51 332 2.54e-45 162
MS.gene59765.t1 AT2G21610 32.450 302 184 6 199 500 56 337 2.77e-45 162
MS.gene59765.t1 AT2G36700 32.459 305 186 5 201 500 44 333 7.02e-45 161
MS.gene59765.t1 AT3G29090 32.915 319 183 6 199 507 7 304 3.67e-44 159
MS.gene59765.t1 AT5G07430 33.226 310 184 8 196 500 64 355 4.62e-41 151
MS.gene59765.t1 AT3G24130 30.882 340 202 9 178 506 17 334 2.10e-40 149
MS.gene59765.t1 AT1G44980 47.904 167 84 1 199 365 78 241 2.98e-39 143
MS.gene59765.t1 AT5G47500 30.086 349 210 9 171 505 34 362 7.76e-39 145
MS.gene59765.t1 AT5G18990 33.449 287 164 6 199 474 29 299 3.21e-38 143
MS.gene59765.t1 AT5G55590 31.447 318 189 7 195 500 78 378 3.33e-37 141
MS.gene59765.t1 AT2G19150 32.372 312 181 9 199 500 41 332 2.20e-36 138
MS.gene59765.t1 AT5G55590 32.700 263 150 6 195 445 78 325 6.92e-35 134
MS.gene59765.t1 AT2G19150 33.463 257 143 8 199 445 41 279 6.50e-33 127
MS.gene59765.t1 AT5G07420 29.126 309 198 7 196 500 64 355 4.72e-31 124
MS.gene59765.t1 AT5G61680 30.435 276 171 8 231 500 98 358 1.72e-29 119
MS.gene59765.t1 AT2G47280 28.754 313 192 9 199 500 35 327 4.67e-29 117
MS.gene59765.t1 AT1G69940 27.759 299 190 10 209 500 76 355 5.19e-28 115
MS.gene59765.t1 AT5G07410 26.846 298 194 8 209 500 76 355 1.09e-27 114
MS.gene59765.t1 AT5G26810 27.647 340 216 9 176 502 13 335 2.76e-27 112

Find 78 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGTGAGTTTGATAATTA+TGG 0.151521 8.3:+55338319 MS.gene59765:CDS
TTGCATAATGCAGCTGTTTC+TGG 0.158204 8.3:+55337788 MS.gene59765:intron
AAGAAGAATATCTTGCTTAT+TGG 0.206210 8.3:+55337392 MS.gene59765:CDS
CCTGCGGACCCCTACCATTT+AGG 0.251261 8.3:+55335201 MS.gene59765:CDS
GTTTATTTGCAATCATATAT+TGG 0.279888 8.3:+55338232 MS.gene59765:CDS
GAAATTCCTGTGTTCTGATT+TGG 0.280507 8.3:-55338109 None:intergenic
TCTTCTGCCTTCAAATTATC+AGG 0.287742 8.3:-55337305 None:intergenic
CTGGTGCTATAACTCATAAT+AGG 0.296276 8.3:+55338344 MS.gene59765:CDS
GAAACTGCAACGGTAGAATA+TGG 0.299226 8.3:-55337903 None:intergenic
GGTGATTTAATTCAACCTTC+TGG 0.303001 8.3:+55338253 MS.gene59765:CDS
AGAATGGAACGACATAGTTT+TGG 0.342470 8.3:-55335159 None:intergenic
GTGAAGTTCCAAGCTTGATT+AGG 0.372862 8.3:-55338397 None:intergenic
GATTGAAGCTGCAGATGATT+TGG 0.384083 8.3:+55338147 MS.gene59765:CDS
TATGTTATTGTGAGTCTTGT+TGG 0.384776 8.3:+55337239 MS.gene59765:CDS
ACAGAAAACTACACTTCTAT+TGG 0.387214 8.3:+55337263 MS.gene59765:CDS
GAATTTCAATACAAAATTGT+AGG 0.393628 8.3:+55338125 MS.gene59765:CDS
GTGTAAGGTACATCAGTATC+AGG 0.398032 8.3:-55338454 None:intergenic
AACCTTCTGGATGGTTAGAA+TGG 0.401213 8.3:+55338266 MS.gene59765:CDS
TTTCTTATCTATGTCAAAGA+AGG 0.432591 8.3:+55337335 MS.gene59765:CDS
CACCGATGTGTTGGTGGAAA+AGG 0.434972 8.3:+55335434 MS.gene59765:CDS
TGGTGCTATAACTCATAATA+GGG 0.435985 8.3:+55338345 MS.gene59765:CDS
TACAGAGAATGTGACATCTA+TGG 0.441861 8.3:+55337971 MS.gene59765:CDS
AGCTGCACCTGATAATTTGA+AGG 0.445519 8.3:+55337298 MS.gene59765:CDS
AATTCAACTAAGAATTACTT+AGG 0.450056 8.3:+55338181 MS.gene59765:CDS
AAATGTAGTCCTTCAGTGTA+AGG 0.452995 8.3:-55338469 None:intergenic
CTGACCTTGTAAGCCTTAGT+AGG 0.460092 8.3:-55335654 None:intergenic
TAATGACTCCTAATCAAGCT+TGG 0.467954 8.3:+55338389 MS.gene59765:CDS
GACCATGGAAAGTGTATTCA+AGG 0.469948 8.3:+55338206 MS.gene59765:CDS
CAAACAAGGTCTTCCTACTA+AGG 0.479222 8.3:+55335641 MS.gene59765:CDS
TGTTTCTTACTGCTACTGCT+TGG 0.479557 8.3:-55337868 None:intergenic
TGTAGAATTGTCTGAGGGAA+TGG 0.482417 8.3:-55337952 None:intergenic
CGCAGGAGAGGAACGGATTG+AGG 0.487599 8.3:-55335184 None:intergenic
GGAAATCATAGTGTGGTAGA+TGG 0.488932 8.3:+55337443 MS.gene59765:CDS
TAGAATGGAATGGAACAGTT+GGG 0.490562 8.3:+55338281 MS.gene59765:CDS
CCTTTCGTGTCTTATACAGT+TGG 0.500763 8.3:-55337174 None:intergenic
CCTAAATGGTAGGGGTCCGC+AGG 0.501953 8.3:-55335201 None:intergenic
CAATTGTATAGTATTTCACT+TGG 0.503200 8.3:+55335570 MS.gene59765:CDS
ATCATAGTGTGGTAGATGGT+TGG 0.507535 8.3:+55337447 MS.gene59765:CDS
AATATCTTGCTTATTGGTGA+TGG 0.511844 8.3:+55337398 MS.gene59765:CDS
CTTAAAGAAAGTGAAAATAG+AGG 0.512875 8.3:+55337200 MS.gene59765:CDS
GTATAGTATTTCACTTGGGT+TGG 0.513615 8.3:+55335575 MS.gene59765:CDS
GATAATTTGAAGGCAGAAGA+TGG 0.515759 8.3:+55337308 MS.gene59765:CDS
GGAACGGATTGAGGAGAGAA+TGG 0.517254 8.3:-55335175 None:intergenic
CTAAGTAATCTACATTGAGC+TGG 0.522297 8.3:-55335362 None:intergenic
TCTTCCTACTAAGGCTTACA+AGG 0.530926 8.3:+55335650 MS.gene59765:CDS
ATTAACAAGACTTGCATCAC+AGG 0.533192 8.3:+55337422 MS.gene59765:CDS
TTCTACCGTTGCAGTTTCGA+AGG 0.533305 8.3:+55337908 MS.gene59765:CDS
GTCCTTGAATACACTTTCCA+TGG 0.545707 8.3:-55338208 None:intergenic
ATTTAATTCAACCTTCTGGA+TGG 0.546192 8.3:+55338257 MS.gene59765:CDS
TAACAACACCGATGTGTTGG+TGG 0.552935 8.3:+55335428 MS.gene59765:CDS
CAGCTGATTTAAGTTCATCT+CGG 0.554463 8.3:-55335392 None:intergenic
ATGGTAGGGGTCCGCAGGAG+AGG 0.559423 8.3:-55335196 None:intergenic
TCTGGATGGTTAGAATGGAA+TGG 0.564904 8.3:+55338271 MS.gene59765:CDS
TTAGAATGGAATGGAACAGT+TGG 0.566790 8.3:+55338280 MS.gene59765:CDS
AAAGGTTGAAGAATTAAAAG+TGG 0.571478 8.3:-55337471 None:intergenic
CTCAATCCGTTCCTCTCCTG+CGG 0.578819 8.3:+55335185 MS.gene59765:CDS
AAGCGTAAGACTCGCAAACA+AGG 0.581727 8.3:+55335627 MS.gene59765:CDS
TGGAGAGAGATTTATAGCAG+TGG 0.584956 8.3:+55337808 MS.gene59765:CDS
AGGGGTCCGCAGGAGAGGAA+CGG 0.590667 8.3:-55335191 None:intergenic
TTCCATTCTAACCATCCAGA+AGG 0.594789 8.3:-55338268 None:intergenic
CTATTATGAGTTATAGCACC+AGG 0.598060 8.3:-55338343 None:intergenic
AATGGACATAGAGTGTGTCT+TGG 0.606022 8.3:-55337934 None:intergenic
AATTGTATAGTATTTCACTT+GGG 0.612303 8.3:+55335571 MS.gene59765:CDS
TGAAACTTATCTTAGTGCTG+TGG 0.613599 8.3:+55335458 MS.gene59765:CDS
GAGTTTGATAATTATGGACC+TGG 0.614496 8.3:+55338325 MS.gene59765:CDS
ACTGATGTACCTTACACTGA+AGG 0.618967 8.3:+55338460 MS.gene59765:CDS
TTCTCTGTAGAATTGTCTGA+GGG 0.620308 8.3:-55337957 None:intergenic
ATTCTCTGTAGAATTGTCTG+AGG 0.621472 8.3:-55337958 None:intergenic
AGAATTACTTAGGAAGACCA+TGG 0.635888 8.3:+55338191 MS.gene59765:CDS
ACTGATCCAAATCAGAACAC+AGG 0.639047 8.3:+55338103 MS.gene59765:CDS
TAACTCATAATAGGGTACAA+TGG 0.642097 8.3:+55338353 MS.gene59765:CDS
ATAACATTCAGAAACACAGC+AGG 0.642782 8.3:+55337833 MS.gene59765:CDS
CATAATAGGGTACAATGGCA+CGG 0.649224 8.3:+55338358 MS.gene59765:CDS
GGTATCCTTCGAAACTGCAA+CGG 0.649403 8.3:-55337913 None:intergenic
AATTGTGTAACATTACTCAA+CGG 0.661988 8.3:-55335552 None:intergenic
CATCACAGGAAATCATAGTG+TGG 0.687415 8.3:+55337436 MS.gene59765:CDS
CCAACTGTATAAGACACGAA+AGG 0.694183 8.3:+55337174 MS.gene59765:CDS
GTTGCAAGATTGAAGTACTG+CGG 0.720720 8.3:-55338023 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTAATGAAACAAAATA+AGG - chr8.3:55335735-55335754 None:intergenic 10.0%
!! TCAAAAATAAATATTTGTAA+GGG - chr8.3:55335799-55335818 None:intergenic 10.0%
!!! GTAATTGTTTTTTAATTTTT+TGG - chr8.3:55336968-55336987 None:intergenic 10.0%
!! AAAAATTCATATGAATATGA+TGG + chr8.3:55336418-55336437 MS.gene59765:intron 15.0%
!! AACTAAAACAAATAAAAAGA+AGG + chr8.3:55336442-55336461 MS.gene59765:intron 15.0%
!! ACAAGTTAATAATTTAAGTA+TGG + chr8.3:55336496-55336515 MS.gene59765:intron 15.0%
!! ACATAAATTAAATGCATTTA+AGG - chr8.3:55336996-55337015 None:intergenic 15.0%
!! ACTAAAACAAATAAAAAGAA+GGG + chr8.3:55336443-55336462 MS.gene59765:intron 15.0%
!! CTCAAAAATAAATATTTGTA+AGG - chr8.3:55335800-55335819 None:intergenic 15.0%
!! TTAATTTAGTTACATTATGA+AGG + chr8.3:55336036-55336055 MS.gene59765:intron 15.0%
!!! AAATATATGTGTTCATTTTA+TGG + chr8.3:55336063-55336082 MS.gene59765:intron 15.0%
!! AATTCAACTAAGAATTACTT+AGG + chr8.3:55338181-55338200 MS.gene59765:CDS 20.0%
!! AATTGTATAGTATTTCACTT+GGG + chr8.3:55335571-55335590 MS.gene59765:CDS 20.0%
!! ATAACGTAAAATATGTTAAC+TGG + chr8.3:55336728-55336747 MS.gene59765:intron 20.0%
!! ATTTAACAGTAACATTATGA+AGG + chr8.3:55337062-55337081 MS.gene59765:intron 20.0%
!! ATTTATAAAGTAATGCTCTT+GGG - chr8.3:55335863-55335882 None:intergenic 20.0%
!! GAATTTCAATACAAAATTGT+AGG + chr8.3:55338125-55338144 MS.gene59765:CDS 20.0%
!! GTTTATTTGCAATCATATAT+TGG + chr8.3:55338232-55338251 MS.gene59765:CDS 20.0%
!! TAAGTATGGAAATAATTCAT+TGG + chr8.3:55336510-55336529 MS.gene59765:intron 20.0%
!! TGCATTATGCAAATAAATTA+AGG - chr8.3:55337780-55337799 None:intergenic 20.0%
!! TTATGCAAATAAATTAAGGT+CGG - chr8.3:55337776-55337795 None:intergenic 20.0%
!! TTGTAACAAAACAAAAGTAA+CGG - chr8.3:55336321-55336340 None:intergenic 20.0%
!!! AATCATTATACTTGTTTTGA+TGG + chr8.3:55335486-55335505 MS.gene59765:CDS 20.0%
!!! ATCATTATACTTGTTTTGAT+GGG + chr8.3:55335487-55335506 MS.gene59765:CDS 20.0%
!!! ATTTTACATTAGGAAATACT+TGG + chr8.3:55338428-55338447 MS.gene59765:CDS 20.0%
!!! CACAACAATATTTCTTTTTA+TGG - chr8.3:55336652-55336671 None:intergenic 20.0%
!!! TTATTGAGAATTTTCCTAAA+TGG - chr8.3:55335218-55335237 None:intergenic 20.0%
! AAAGGTTGAAGAATTAAAAG+TGG - chr8.3:55337474-55337493 None:intergenic 25.0%
! AAATATAACATCTCACATCT+CGG + chr8.3:55337639-55337658 MS.gene59765:intron 25.0%
! AAGAAGAATATCTTGCTTAT+TGG + chr8.3:55337392-55337411 MS.gene59765:CDS 25.0%
! AATTGTGTAACATTACTCAA+CGG - chr8.3:55335555-55335574 None:intergenic 25.0%
! ACGCAAATTAAATGTTTAGT+GGG + chr8.3:55336701-55336720 MS.gene59765:intron 25.0%
! AGCATTACTTTATAAATGTG+AGG + chr8.3:55335866-55335885 MS.gene59765:intron 25.0%
! ATTATATACAAGATGACAAG+TGG - chr8.3:55336940-55336959 None:intergenic 25.0%
! CAATTGTATAGTATTTCACT+TGG + chr8.3:55335570-55335589 MS.gene59765:CDS 25.0%
! CATTTATAAAGTAATGCTCT+TGG - chr8.3:55335864-55335883 None:intergenic 25.0%
! CTTAAAGAAAGTGAAAATAG+AGG + chr8.3:55337200-55337219 MS.gene59765:CDS 25.0%
! TACGCAAATTAAATGTTTAG+TGG + chr8.3:55336700-55336719 MS.gene59765:intron 25.0%
! TTTCTTATCTATGTCAAAGA+AGG + chr8.3:55337335-55337354 MS.gene59765:CDS 25.0%
! TTTGTTTGTTTGTGTCATAA+TGG + chr8.3:55336852-55336871 MS.gene59765:intron 25.0%
!! ACTGTGCTTAATTTTACATT+AGG + chr8.3:55338418-55338437 MS.gene59765:CDS 25.0%
!! CTTGGGTTATTGATTAAAAA+TGG - chr8.3:55335846-55335865 None:intergenic 25.0%
!! GAAAAAATTTGACTTACCAA+AGG - chr8.3:55337492-55337511 None:intergenic 25.0%
!! GTTCGTATATATGCTTTATT+AGG - chr8.3:55337558-55337577 None:intergenic 25.0%
!! TATGGTGAGTTTGATAATTA+TGG + chr8.3:55338319-55338338 MS.gene59765:CDS 25.0%
!!! AAGAAGAAGTTGATATAACT+GGG - chr8.3:55336912-55336931 None:intergenic 25.0%
!!! ACCTTAATAAGCTTTTTAAG+TGG - chr8.3:55335684-55335703 None:intergenic 25.0%
!!! ACTTTTAATTCTTCAACCTT+TGG + chr8.3:55337473-55337492 MS.gene59765:CDS 25.0%
!!! ATCCTCACATTTTTTTTTTG+TGG - chr8.3:55336351-55336370 None:intergenic 25.0%
!!! GAATACTTTGGATAGTTTTT+TGG - chr8.3:55335259-55335278 None:intergenic 25.0%
!!! TAAGAAGAAGTTGATATAAC+TGG - chr8.3:55336913-55336932 None:intergenic 25.0%
AAAAATGCTATAACAGCACA+AGG + chr8.3:55338076-55338095 MS.gene59765:CDS 30.0%
AAACTCTTAGAAATTGTGGT+TGG + chr8.3:55336166-55336185 MS.gene59765:intron 30.0%
AACTCTTAGAAATTGTGGTT+GGG + chr8.3:55336167-55336186 MS.gene59765:intron 30.0%
AATATCTTGCTTATTGGTGA+TGG + chr8.3:55337398-55337417 MS.gene59765:CDS 30.0%
ACAGAAAACTACACTTCTAT+TGG + chr8.3:55337263-55337282 MS.gene59765:CDS 30.0%
ATTTAATTCAACCTTCTGGA+TGG + chr8.3:55338257-55338276 MS.gene59765:CDS 30.0%
CACCACAAAAAAAAAATGTG+AGG + chr8.3:55336346-55336365 MS.gene59765:intron 30.0%
CATTTGTCACATTGTGAATA+AGG - chr8.3:55335952-55335971 None:intergenic 30.0%
GAGAAAGTCTTTGAATACTT+TGG - chr8.3:55335271-55335290 None:intergenic 30.0%
GCCACTTAAAAAGCTTATTA+AGG + chr8.3:55335680-55335699 MS.gene59765:CDS 30.0%
TAACTCATAATAGGGTACAA+TGG + chr8.3:55338353-55338372 MS.gene59765:CDS 30.0%
TTAGAGTCTCTTCTTCTAAA+AGG + chr8.3:55336142-55336161 MS.gene59765:intron 30.0%
! AGAATTTTCCTAAATGGTAG+GGG - chr8.3:55335212-55335231 None:intergenic 30.0%
! ATGTGTTCATTTTATGGCTA+AGG + chr8.3:55336069-55336088 MS.gene59765:intron 30.0%
! ATTTTTACTCAAAGCTTGAG+TGG - chr8.3:55335601-55335620 None:intergenic 30.0%
! CATTTTTGCAAGAAAACCAA+TGG + chr8.3:55338045-55338064 MS.gene59765:CDS 30.0%
! GAGAATTTTCCTAAATGGTA+GGG - chr8.3:55335213-55335232 None:intergenic 30.0%
! TATGTTATTGTGAGTCTTGT+TGG + chr8.3:55337239-55337258 MS.gene59765:CDS 30.0%
! TGAGAATTTTCCTAAATGGT+AGG - chr8.3:55335214-55335233 None:intergenic 30.0%
!! AATTATTAACTTGTGCCTTG+AGG - chr8.3:55336490-55336509 None:intergenic 30.0%
!! AGAAGAAGTTGATATAACTG+GGG - chr8.3:55336911-55336930 None:intergenic 30.0%
!! ATTATTAACTTGTGCCTTGA+GGG - chr8.3:55336489-55336508 None:intergenic 30.0%
!! TGGTGCTATAACTCATAATA+GGG + chr8.3:55338345-55338364 MS.gene59765:CDS 30.0%
!!! ATAGTTTTTTGGCTTGTTTC+AGG - chr8.3:55335248-55335267 None:intergenic 30.0%
!!! GCATTTTTTTGATTTGCCAT+TGG - chr8.3:55338064-55338083 None:intergenic 30.0%
!!! GGGTTAGATACACTTTTTTA+TGG + chr8.3:55338301-55338320 MS.gene59765:CDS 30.0%
!!! TTATACTTGTTTTGATGGGT+TGG + chr8.3:55335491-55335510 MS.gene59765:CDS 30.0%
AAATGTAGTCCTTCAGTGTA+AGG - chr8.3:55338472-55338491 None:intergenic 35.0%
AAGAAGAGACTCTAAACTAG+CGG - chr8.3:55336137-55336156 None:intergenic 35.0%
AAGTTAGCTAAAACCTAGGT+TGG - chr8.3:55336780-55336799 None:intergenic 35.0%
AGAATTACTTAGGAAGACCA+TGG + chr8.3:55338191-55338210 MS.gene59765:CDS 35.0%
AGGCAAACTCTTAGAAATTG+TGG + chr8.3:55336162-55336181 MS.gene59765:intron 35.0%
ATAACATTCAGAAACACAGC+AGG + chr8.3:55337833-55337852 MS.gene59765:CDS 35.0%
ATCTCGAAAGTCTTACATCA+TGG + chr8.3:55336008-55336027 MS.gene59765:intron 35.0%
ATTAACAAGACTTGCATCAC+AGG + chr8.3:55337422-55337441 MS.gene59765:CDS 35.0%
ATTCTCTGTAGAATTGTCTG+AGG - chr8.3:55337961-55337980 None:intergenic 35.0%
ATTTCTTAACATGTGCCCTA+GGG + chr8.3:55336611-55336630 MS.gene59765:intron 35.0%
CTAAGTAATCTACATTGAGC+TGG - chr8.3:55335365-55335384 None:intergenic 35.0%
CTATTATGAGTTATAGCACC+AGG - chr8.3:55338346-55338365 None:intergenic 35.0%
CTGGCAATGTGTTTATAAGT+GGG - chr8.3:55336237-55336256 None:intergenic 35.0%
CTTCAGATTAAAGTCGTATC+TGG - chr8.3:55337693-55337712 None:intergenic 35.0%
GAAATTCCTGTGTTCTGATT+TGG - chr8.3:55338112-55338131 None:intergenic 35.0%
GATAATTTGAAGGCAGAAGA+TGG + chr8.3:55337308-55337327 MS.gene59765:CDS 35.0%
GGTGATTTAATTCAACCTTC+TGG + chr8.3:55338253-55338272 MS.gene59765:CDS 35.0%
GTAAGACTTTCGAGATTTCA+AGG - chr8.3:55336004-55336023 None:intergenic 35.0%
GTATAGTATTTCACTTGGGT+TGG + chr8.3:55335575-55335594 MS.gene59765:CDS 35.0%
GTTAATGATGTGTACGTGTT+AGG - chr8.3:55337596-55337615 None:intergenic 35.0%
TAATGACTCCTAATCAAGCT+TGG + chr8.3:55338389-55338408 MS.gene59765:CDS 35.0%
TACAGAGAATGTGACATCTA+TGG + chr8.3:55337971-55337990 MS.gene59765:CDS 35.0%
TAGAATGGAATGGAACAGTT+GGG + chr8.3:55338281-55338300 MS.gene59765:CDS 35.0%
TCAGAAGTTAGCTAAAACCT+AGG - chr8.3:55336784-55336803 None:intergenic 35.0%
TCTCGAAAGTCTTACATCAT+GGG + chr8.3:55336009-55336028 MS.gene59765:intron 35.0%
TCTTCTGCCTTCAAATTATC+AGG - chr8.3:55337308-55337327 None:intergenic 35.0%
TGAAACTTATCTTAGTGCTG+TGG + chr8.3:55335458-55335477 MS.gene59765:CDS 35.0%
TGGCAATGTGTTTATAAGTG+GGG - chr8.3:55336236-55336255 None:intergenic 35.0%
TGTGAGGATTAAAATGAGCT+TGG + chr8.3:55335882-55335901 MS.gene59765:intron 35.0%
TTAGAATGGAATGGAACAGT+TGG + chr8.3:55338280-55338299 MS.gene59765:CDS 35.0%
TTCTCTGTAGAATTGTCTGA+GGG - chr8.3:55337960-55337979 None:intergenic 35.0%
! ACGACTTTAATCTGAAGTGT+CGG + chr8.3:55337696-55337715 MS.gene59765:intron 35.0%
! AGAATGGAACGACATAGTTT+TGG - chr8.3:55335162-55335181 None:intergenic 35.0%
! CAGCTGATTTAAGTTCATCT+CGG - chr8.3:55335395-55335414 None:intergenic 35.0%
! GAGTTTGATAATTATGGACC+TGG + chr8.3:55338325-55338344 MS.gene59765:CDS 35.0%
! TTTTAACAACACCGATGTGT+TGG + chr8.3:55335425-55335444 MS.gene59765:CDS 35.0%
!! CTGGTGCTATAACTCATAAT+AGG + chr8.3:55338344-55338363 MS.gene59765:CDS 35.0%
!! GGCACAGTTGATTTCATTTT+CGG + chr8.3:55337992-55338011 MS.gene59765:CDS 35.0%
AACCTTCTGGATGGTTAGAA+TGG + chr8.3:55338266-55338285 MS.gene59765:CDS 40.0%
AATGGACATAGAGTGTGTCT+TGG - chr8.3:55337937-55337956 None:intergenic 40.0%
ACTGATCCAAATCAGAACAC+AGG + chr8.3:55338103-55338122 MS.gene59765:CDS 40.0%
ACTGATGTACCTTACACTGA+AGG + chr8.3:55338460-55338479 MS.gene59765:CDS 40.0%
AGCTGCACCTGATAATTTGA+AGG + chr8.3:55337298-55337317 MS.gene59765:CDS 40.0%
ATCATAGTGTGGTAGATGGT+TGG + chr8.3:55337447-55337466 MS.gene59765:CDS 40.0%
ATGTTAACTGGCTAGATCCA+AGG + chr8.3:55336740-55336759 MS.gene59765:intron 40.0%
CAAACAAGGTCTTCCTACTA+AGG + chr8.3:55335641-55335660 MS.gene59765:CDS 40.0%
CATAATAGGGTACAATGGCA+CGG + chr8.3:55338358-55338377 MS.gene59765:CDS 40.0%
CATCACAGGAAATCATAGTG+TGG + chr8.3:55337436-55337455 MS.gene59765:CDS 40.0%
CATTTCTTAACATGTGCCCT+AGG + chr8.3:55336610-55336629 MS.gene59765:intron 40.0%
CCAACTGTATAAGACACGAA+AGG + chr8.3:55337174-55337193 MS.gene59765:CDS 40.0%
CCACTTATAAACACATTGCC+AGG + chr8.3:55336235-55336254 MS.gene59765:intron 40.0%
CCTGGCAATGTGTTTATAAG+TGG - chr8.3:55336238-55336257 None:intergenic 40.0%
CCTTTCGTGTCTTATACAGT+TGG - chr8.3:55337177-55337196 None:intergenic 40.0%
CTGGTGTTCAACATATGTGT+TGG - chr8.3:55337674-55337693 None:intergenic 40.0%
GAAACTGCAACGGTAGAATA+TGG - chr8.3:55337906-55337925 None:intergenic 40.0%
GATCCAAGGAGGTTAAATTC+TGG + chr8.3:55336754-55336773 MS.gene59765:intron 40.0%
GATTGAAGCTGCAGATGATT+TGG + chr8.3:55338147-55338166 MS.gene59765:CDS 40.0%
GGAAATCATAGTGTGGTAGA+TGG + chr8.3:55337443-55337462 MS.gene59765:CDS 40.0%
GGCAATGTGTTTATAAGTGG+GGG - chr8.3:55336235-55336254 None:intergenic 40.0%
GTCCTTGAATACACTTTCCA+TGG - chr8.3:55338211-55338230 None:intergenic 40.0%
GTGAAGTTCCAAGCTTGATT+AGG - chr8.3:55338400-55338419 None:intergenic 40.0%
GTGTAAGGTACATCAGTATC+AGG - chr8.3:55338457-55338476 None:intergenic 40.0%
TCGAGATTTCAAGGAACACA+GGG - chr8.3:55335995-55336014 None:intergenic 40.0%
TCTGGATGGTTAGAATGGAA+TGG + chr8.3:55338271-55338290 MS.gene59765:CDS 40.0%
TCTTCCTACTAAGGCTTACA+AGG + chr8.3:55335650-55335669 MS.gene59765:CDS 40.0%
TGGAGAGAGATTTATAGCAG+TGG + chr8.3:55337808-55337827 MS.gene59765:CDS 40.0%
TGTAGAATTGTCTGAGGGAA+TGG - chr8.3:55337955-55337974 None:intergenic 40.0%
TGTTTCTTACTGCTACTGCT+TGG - chr8.3:55337871-55337890 None:intergenic 40.0%
TTCCATTCTAACCATCCAGA+AGG - chr8.3:55338271-55338290 None:intergenic 40.0%
TTCGAGATTTCAAGGAACAC+AGG - chr8.3:55335996-55336015 None:intergenic 40.0%
! AACCTTTTCCACCAACACAT+CGG - chr8.3:55335439-55335458 None:intergenic 40.0%
! GACCATGGAAAGTGTATTCA+AGG + chr8.3:55338206-55338225 MS.gene59765:CDS 40.0%
! TAACTTGTATCCTTCCCTCA+AGG + chr8.3:55336472-55336491 MS.gene59765:intron 40.0%
! TGATTTCATTTTCGGCAACG+CGG + chr8.3:55338000-55338019 MS.gene59765:CDS 40.0%
! TTGCATAATGCAGCTGTTTC+TGG + chr8.3:55337788-55337807 MS.gene59765:intron 40.0%
!! AAGTTGATATAACTGGGGTG+GGG - chr8.3:55336906-55336925 None:intergenic 40.0%
!! ACCTCACAAGTCGATTTTGT+GGG - chr8.3:55336204-55336223 None:intergenic 40.0%
!! AGAAGTTGATATAACTGGGG+TGG - chr8.3:55336908-55336927 None:intergenic 40.0%
!! GAAGTTGATATAACTGGGGT+GGG - chr8.3:55336907-55336926 None:intergenic 40.0%
!! GTTGCAAGATTGAAGTACTG+CGG - chr8.3:55338026-55338045 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTA+GGG - chr8.3:55336629-55336648 None:intergenic 40.0%
!! TTAACCGCAAGAGTGTTGTT+TGG + chr8.3:55335916-55335935 MS.gene59765:intron 40.0%
AAGAGACTCTAAACTAGCGG+CGG - chr8.3:55336134-55336153 None:intergenic 45.0%
AAGCGTAAGACTCGCAAACA+AGG + chr8.3:55335627-55335646 MS.gene59765:CDS 45.0%
CATGGTTAAGAGAAGTGGAG+TGG - chr8.3:55335305-55335324 None:intergenic 45.0%
CGAGATTTCAAGGAACACAG+GGG - chr8.3:55335994-55336013 None:intergenic 45.0%
CTGACCTTGTAAGCCTTAGT+AGG - chr8.3:55335657-55335676 None:intergenic 45.0%
GATGATTCCAGTGAGATGAG+TGG - chr8.3:55336112-55336131 None:intergenic 45.0%
GCTTCCAAACAACACTCTTG+CGG - chr8.3:55335923-55335942 None:intergenic 45.0%
GGTATCCTTCGAAACTGCAA+CGG - chr8.3:55337916-55337935 None:intergenic 45.0%
GGTTAAATTCTGGCCAACCT+AGG + chr8.3:55336764-55336783 MS.gene59765:intron 45.0%
TAACAACACCGATGTGTTGG+TGG + chr8.3:55335428-55335447 MS.gene59765:CDS 45.0%
TCTTAACCATGCCGAGATTG+CGG + chr8.3:55335314-55335333 MS.gene59765:intron 45.0%
TGAGTAGTATCTCAGTCACG+TGG + chr8.3:55336807-55336826 MS.gene59765:intron 45.0%
TGGCCAGAATTTAACCTCCT+TGG - chr8.3:55336760-55336779 None:intergenic 45.0%
TTAACTGGCTAGATCCAAGG+AGG + chr8.3:55336743-55336762 MS.gene59765:intron 45.0%
TTAAGAGTCCCACATCGAAC+AGG - chr8.3:55336267-55336286 None:intergenic 45.0%
TTCTACCGTTGCAGTTTCGA+AGG + chr8.3:55337908-55337927 MS.gene59765:CDS 45.0%
! CGATTTTGTGGGGTTGAGTT+AGG - chr8.3:55336193-55336212 None:intergenic 45.0%
! CGTGTTAGGTGTCATATGTC+TGG - chr8.3:55337582-55337601 None:intergenic 45.0%
!! CAAAATCGACTTGTGAGGTG+AGG + chr8.3:55336205-55336224 MS.gene59765:intron 45.0%
!! CACCTCACAAGTCGATTTTG+TGG - chr8.3:55336205-55336224 None:intergenic 45.0%
!! CCTCACAAGTCGATTTTGTG+GGG - chr8.3:55336203-55336222 None:intergenic 45.0%
!! GTAGTGTTAACTTGTGCCCT+AGG - chr8.3:55336630-55336649 None:intergenic 45.0%
!! TTAACTTGTGCCTTGAGGGA+AGG - chr8.3:55336485-55336504 None:intergenic 45.0%
!! TATAAAGAATAAATATTAAA+CGG + chr8.3:55336390-55336409 MS.gene59765:intron 5.0%
ATCGTCTAGTGCCGCAATCT+CGG - chr8.3:55335328-55335347 None:intergenic 50.0%
CTCGGCATGGTTAAGAGAAG+TGG - chr8.3:55335310-55335329 None:intergenic 50.0%
GTGAGATGAGTGGAGCAGAA+TGG - chr8.3:55336102-55336121 None:intergenic 50.0%
TTCTGCTCCACTCATCTCAC+TGG + chr8.3:55336102-55336121 MS.gene59765:intron 50.0%
TTGATATAACTGGGGTGGGG+TGG - chr8.3:55336903-55336922 None:intergenic 50.0%
! CACCGATGTGTTGGTGGAAA+AGG + chr8.3:55335434-55335453 MS.gene59765:CDS 50.0%
!! CCCCACAAAATCGACTTGTG+AGG + chr8.3:55336200-55336219 MS.gene59765:intron 50.0%
!! GGAACGGATTGAGGAGAGAA+TGG - chr8.3:55335178-55335197 None:intergenic 50.0%
!!! GCTACTGCTTGGTGCTTTTG+AGG - chr8.3:55337860-55337879 None:intergenic 50.0%
ACATCGAACAGGAGAAGGCC+TGG - chr8.3:55336256-55336275 None:intergenic 55.0%
AGGCCTTCTCCTGTTCGATG+TGG + chr8.3:55336255-55336274 MS.gene59765:intron 55.0%
CTCAATCCGTTCCTCTCCTG+CGG + chr8.3:55335185-55335204 MS.gene59765:CDS 55.0%
GGCCTTCTCCTGTTCGATGT+GGG + chr8.3:55336256-55336275 MS.gene59765:intron 55.0%
GTCCCACATCGAACAGGAGA+AGG - chr8.3:55336261-55336280 None:intergenic 55.0%
CCTAAATGGTAGGGGTCCGC+AGG - chr8.3:55335204-55335223 None:intergenic 60.0%
CCTGCGGACCCCTACCATTT+AGG + chr8.3:55335201-55335220 MS.gene59765:CDS 60.0%
CTAGTGCCGCAATCTCGGCA+TGG - chr8.3:55335323-55335342 None:intergenic 60.0%
!! CGCAGGAGAGGAACGGATTG+AGG - chr8.3:55335187-55335206 None:intergenic 60.0%
AGGGGTCCGCAGGAGAGGAA+CGG - chr8.3:55335194-55335213 None:intergenic 65.0%
ATGGTAGGGGTCCGCAGGAG+AGG - chr8.3:55335199-55335218 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 55335140 55338501 55335140 ID=MS.gene59765
chr8.3 mRNA 55335140 55338501 55335140 ID=MS.gene59765.t1;Parent=MS.gene59765
chr8.3 exon 55335140 55335272 55335140 ID=MS.gene59765.t1.exon1;Parent=MS.gene59765.t1
chr8.3 CDS 55335140 55335272 55335140 ID=cds.MS.gene59765.t1;Parent=MS.gene59765.t1
chr8.3 exon 55335343 55335701 55335343 ID=MS.gene59765.t1.exon2;Parent=MS.gene59765.t1
chr8.3 CDS 55335343 55335701 55335343 ID=cds.MS.gene59765.t1;Parent=MS.gene59765.t1
chr8.3 exon 55337140 55337494 55337140 ID=MS.gene59765.t1.exon3;Parent=MS.gene59765.t1
chr8.3 CDS 55337140 55337494 55337140 ID=cds.MS.gene59765.t1;Parent=MS.gene59765.t1
chr8.3 exon 55337801 55338501 55337801 ID=MS.gene59765.t1.exon4;Parent=MS.gene59765.t1
chr8.3 CDS 55337801 55338501 55337801 ID=cds.MS.gene59765.t1;Parent=MS.gene59765.t1
Gene Sequence

>MS.gene59765

ATGCAAATCAACATTATATCCAAAACTATGTCGTTCCATTCTCTCCTCAATCCGTTCCTCTCCTGCGGACCCCTACCATTTAGGAAAATTCTCAATAAAACAAAGCCTGAAACAAGCCAAAAAACTATCCAAAGTATTCAAAGACTTTCTCAAAAAACATCACCACTCCACTTCTCTTAACCATGCCGAGATTGCGGCACTAGACGATTGTAGTGAACTAAACCAGCTCAATGTAGATTACTTAGAGTTAGTCCGAGATGAACTTAAATCAGCTGATACTTCATCTTTTAACAACACCGATGTGTTGGTGGAAAAGGTTGAAACTTATCTTAGTGCTGTGGCTACTAATCATTATACTTGTTTTGATGGGTTGGTTGTGACTAAAAGTAACATTGCTAATGCACTTGCTGTGCCGTTGAGTAATGTTACACAATTGTATAGTATTTCACTTGGGTTGGCCACTCAAGCTTTGAGTAAAAATGTTAAGAAGCGTAAGACTCGCAAACAAGGTCTTCCTACTAAGGCTTACAAGGTCAGACAGCCACTTAAAAAGCTTATTAAGGTAACTATTTTCTTTATCTATATTTGACTACCTTATTTTGTTTCATTAAATATGTTTTTTTTTTGTCAAAAAAAAAATTAAATATGTTTTTTAGCCCTTACAAATATTTATTTTTGAGTTTGATTTCTATAAGTATTTTATCCATTTTTAATCAATAACCCAAGAGCATTACTTTATAAATGTGAGGATTAAAATGAGCTTGGCTCTACAAACTTTAACCGCAAGAGTGTTGTTTGGAAGCAGTAGACCTTATTCACAATGTGACAAATGCAAAGTTTGCATGTGTATGCCCCTGTGTTCCTTGAAATCTCGAAAGTCTTACATCATGGGATTATTAATTTAGTTACATTATGAAGGAGCAAAATATATGTGTTCATTTTATGGCTAAGGATCGTGTCCATTCTGCTCCACTCATCTCACTGGAATCATCCGCCGCTAGTTTAGAGTCTCTTCTTCTAAAAGGCAAACTCTTAGAAATTGTGGTTGGGCCTAACTCAACCCCACAAAATCGACTTGTGAGGTGAGGATTGCCCCCACTTATAAACACATTGCCAGGCCTTCTCCTGTTCGATGTGGGACTCTTAACACAAACTAGTTATTTAGTGTTTTTTTTCTTCCGTTACTTTTGTTTTGTTACAATGTAACACCACAAAAAAAAAATGTGAGGATTAAAATAGATAATTTTTTTTATAAAGAATAAATATTAAACGGCGATCAAAAATTCATATGAATATGATGGTAACTAAAACAAATAAAAAGAAGGGAAATATTAACTTGTATCCTTCCCTCAAGGCACAAGTTAATAATTTAAGTATGGAAATAATTCATTGGAATGTGTGTATTGTATTTAATCTTTAACTTTTTAATCTATTAAATGCATAAAATTGTAAAAAATATTTTTTCTTTTGCATTTCTTAACATGTGCCCTAGGGCACAAGTTAACACTACCATAAAAAGAAATATTGTTGTGTTATCGAAATCTAATCAGCACTTAGTCATACGCAAATTAAATGTTTAGTGGGACTAATAACGTAAAATATGTTAACTGGCTAGATCCAAGGAGGTTAAATTCTGGCCAACCTAGGTTTTAGCTAACTTCTGACTCTGAGTAGTATCTCAGTCACGTGGTTTCATGTTCGTTAATCGTTTGTTTGTTTGTTTGTGTCATAATGGAAAGAACACGTGCAACTCACAAGTACCACCCCACCCCAGTTATATCAACTTCTTCTTATATGCCACTTGTCATCTTGTATATAATATGTTCCAAAAAATTAAAAAACAATTACTTTGTCCTTAAATGCATTTAATTTATGTGTAATGACATTTGAAGCTCATCATAGAAAAGTAATGATAATTATGCATTTAACAGTAACATTATGAAGGTAAAATTGTTAATTGAAATTTGAAAGTCTAAAATTGTAACATTACTCTATTGCAGCTTCTTCACACGAAACACAGTTGCAATAACTCATCCAACTGTATAAGACACGAAAGGATTCTTAAAGAAAGTGAAAATAGAGGAATTTTACTGAAAGAATATGTTATTGTGAGTCTTGTTGGAACAGAAAACTACACTTCTATTGGTGAAGCTATTGAAGCTGCACCTGATAATTTGAAGGCAGAAGATGGCTATTTTCTTATCTATGTCAAAGAAGGAGTATATGAAGAATATGTTACTATTACAAAACATAAGAAGAATATCTTGCTTATTGGTGATGGTATTAACAAGACTTGCATCACAGGAAATCATAGTGTGGTAGATGGTTGGACCACTTTTAATTCTTCAACCTTTGGTAAGTCAAATTTTTTCAATTGTACTAGCTAGAGATTTTTCAATAAAAAATTAACTATAACCTAATAAAGCATATATACGAACACCAGACATATGACACCTAACACGTACACATCATTAACAAGTTAAGAAAATTAAAGTTATTAAATATAACATCTCACATCTCGGTATTAGACACCAACACATATGTTGAACACCAGATACGACTTTAATCTGAAGTGTCGGTACTACATAACATAGAGTGCAATCATGATAGAACGTTATTTTTCTTGTAATAATCCGACCTTAATTTATTTGCATAATGCAGCTGTTTCTGGAGAGAGATTTATAGCAGTGGACATAACATTCAGAAACACAGCAGGACCTCAAAAGCACCAAGCAGTAGCAGTAAGAAACAATGCAGATCTTTCCATATTCTACCGTTGCAGTTTCGAAGGATACCAAGACACACTCTATGTCCATTCCCTCAGACAATTCTACAGAGAATGTGACATCTATGGCACAGTTGATTTCATTTTCGGCAACGCGGCCGCAGTACTTCAATCTTGCAACATTTTTGCAAGAAAACCAATGGCAAATCAAAAAAATGCTATAACAGCACAAGGTAGAACTGATCCAAATCAGAACACAGGAATTTCAATACAAAATTGTAGGATTGAAGCTGCAGATGATTTGGCTGAAGATTTGAATTCAACTAAGAATTACTTAGGAAGACCATGGAAAGTGTATTCAAGGACTGTTTATTTGCAATCATATATTGGTGATTTAATTCAACCTTCTGGATGGTTAGAATGGAATGGAACAGTTGGGTTAGATACACTTTTTTATGGTGAGTTTGATAATTATGGACCTGGTGCTATAACTCATAATAGGGTACAATGGCACGGTTACAATTTAATGACTCCTAATCAAGCTTGGAACTTCACTGTGCTTAATTTTACATTAGGAAATACTTGGCTTCCTGATACTGATGTACCTTACACTGAAGGACTACATTTAGATAATTGA
Protein sequence

>MS.gene59765.t1

MQINIISKTMSFHSLLNPFLSCGPLPFRKILNKTKPETSQKTIQNDCSELNQLNVDYLELVRDELKSADTSSFNNTDVLVEKVETYLSAVATNHYTCFDGLVVTKSNIANALAVPLSNVTQLYSISLGLATQALSKNVKKRKTRKQGLPTKAYKVRQPLKKLIKLLHTKHSCNNSSNCIRHERILKESENRGILLKEYVIVSLVGTENYTSIGEAIEAAPDNLKAEDGYFLIYVKEGVYEEYVTITKHKKNILLIGDGINKTCITGNHSVVDGWTTFNSSTFAVSGERFIAVDITFRNTAGPQKHQAVAVRNNADLSIFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVLQSCNIFARKPMANQKNAITAQGRTDPNQNTGISIQNCRIEAADDLAEDLNSTKNYLGRPWKVYSRTVYLQSYIGDLIQPSGWLEWNGTVGLDTLFYGEFDNYGPGAITHNRVQWHGYNLMTPNQAWNFTVLNFTLGNTWLPDTDVPYTEGLHLDN