AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59968


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59968.t1 MTR_8g041770 89.423 208 22 0 1 208 234 441 2.58e-135 386
MS.gene59968.t1 MTR_0190s0020 74.519 208 53 0 1 208 235 442 2.13e-110 323
MS.gene59968.t1 MTR_4g022825 62.500 208 75 2 1 207 234 439 1.29e-90 273
MS.gene59968.t1 MTR_3g088420 61.244 209 78 2 1 208 227 433 3.56e-88 266
MS.gene59968.t1 MTR_4g022720 61.722 209 77 2 1 208 229 435 8.04e-88 265
MS.gene59968.t1 MTR_4g022800 61.905 210 76 2 1 208 235 442 1.53e-87 265
MS.gene59968.t1 MTR_4g022670 60.287 209 80 2 1 208 235 441 1.81e-86 262
MS.gene59968.t1 MTR_8g040780 60.194 206 79 2 1 203 22 227 2.28e-86 255
MS.gene59968.t1 MTR_4g094835 60.287 209 77 3 1 204 235 442 1.20e-85 260
MS.gene59968.t1 MTR_4g094832 60.287 209 77 3 1 204 219 426 9.30e-85 257
MS.gene59968.t1 MTR_3g026310 57.820 211 84 2 1 208 240 448 9.43e-85 258
MS.gene59968.t1 MTR_7g026860 59.615 208 80 2 1 208 235 438 3.13e-82 251
MS.gene59968.t1 MTR_4g094842 56.311 206 90 0 1 206 241 446 1.38e-81 249
MS.gene59968.t1 MTR_4g094838 58.373 209 81 3 1 204 221 428 3.88e-81 248
MS.gene59968.t1 MTR_4g094828 49.770 217 99 5 1 208 235 450 1.69e-63 203
MS.gene59968.t1 MTR_4g022760 48.558 208 62 1 1 208 235 397 6.22e-61 195
MS.gene59968.t1 MTR_8g079710 49.761 209 95 5 1 199 228 436 8.43e-60 193
MS.gene59968.t1 MTR_4g022290 47.248 218 102 5 1 206 227 443 4.84e-54 179
MS.gene59968.t1 MTR_4g094825 52.980 151 59 2 55 204 248 387 8.10e-48 161
MS.gene59968.t1 MTR_3g101900 40.191 209 122 3 2 208 241 448 2.41e-46 159
MS.gene59968.t1 MTR_3g102020 40.097 207 121 3 1 205 243 448 5.17e-45 155
MS.gene59968.t1 MTR_3g101930 38.942 208 125 2 1 207 248 454 2.39e-44 153
MS.gene59968.t1 MTR_6g016815 43.269 208 56 4 1 208 236 381 3.00e-44 152
MS.gene59968.t1 MTR_3g101970 38.278 209 126 3 1 207 229 436 4.44e-42 147
MS.gene59968.t1 MTR_3g101960 39.024 205 122 3 1 203 242 445 8.55e-42 147
MS.gene59968.t1 MTR_3g102010 39.048 210 125 3 1 208 243 451 1.50e-41 146
MS.gene59968.t1 MTR_6g034485 68.605 86 27 0 1 86 40 125 2.00e-37 127
MS.gene59968.t1 MTR_5g081960 34.536 194 125 2 16 208 294 486 1.94e-33 125
MS.gene59968.t1 MTR_7g026770 55.769 104 46 0 1 104 198 301 1.28e-32 119
MS.gene59968.t1 MTR_4g015180 36.216 185 116 2 25 208 304 487 6.82e-32 120
MS.gene59968.t1 MTR_3g070170 33.831 201 130 3 6 204 267 466 3.05e-30 116
MS.gene59968.t1 MTR_6g045553 31.250 208 138 3 1 204 265 471 9.05e-27 106
MS.gene59968.t1 MTR_7g026780 47.748 111 33 3 98 208 26 111 1.14e-24 94.0
MS.gene59968.t1 MTR_3g102030 33.129 163 90 3 1 162 176 320 1.88e-21 90.1
MS.gene59968.t1 MTR_7g026870 54.286 70 27 1 53 117 82 151 6.28e-18 77.4
MS.gene59968.t1 MTR_4g007540 25.126 199 144 3 1 195 227 424 1.94e-17 80.1
MS.gene59968.t1 MTR_8g075610 24.631 203 138 3 1 195 216 411 6.31e-16 75.9
MS.gene59968.t1 MTR_2g064495 24.242 198 147 2 1 195 226 423 2.45e-15 73.9
MS.gene59968.t1 MTR_2g105330 24.242 198 147 2 1 195 226 423 5.64e-15 73.2
MS.gene59968.t1 MTR_4g127650 23.364 214 146 3 1 197 236 448 1.63e-14 71.6
MS.gene59968.t1 MTR_3g098980 25.532 188 133 3 2 189 238 418 1.33e-13 68.9
MS.gene59968.t1 MTR_3g026950 26.484 219 123 7 1 192 229 436 3.97e-13 67.8
MS.gene59968.t1 MTR_7g026880 55.556 54 22 1 155 208 2 53 5.78e-13 61.6
MS.gene59968.t1 MTR_3g049150 24.528 212 126 6 1 189 229 429 1.02e-12 66.6
MS.gene59968.t1 MTR_3g049140 24.038 208 137 7 1 192 27 229 2.06e-12 64.7
MS.gene59968.t1 MTR_6g015815 25.000 200 144 3 1 198 258 453 3.44e-12 65.1
MS.gene59968.t1 MTR_4g097090 25.728 206 135 6 1 189 236 440 6.21e-12 64.3
MS.gene59968.t1 MTR_6g015320 25.500 200 138 5 1 195 251 444 7.29e-12 63.9
MS.gene59968.t1 MTR_8g096530 27.848 158 105 3 2 157 232 382 1.63e-11 62.8
MS.gene59968.t1 MTR_6g015315 24.883 213 130 8 1 198 248 445 1.74e-11 62.8
MS.gene59968.t1 MTR_7g014250 25.000 208 141 6 1 199 249 450 3.82e-11 62.0
MS.gene59968.t1 MTR_6g015850 25.871 201 140 5 1 198 256 450 4.09e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59968.t1 AT5G67150 41.429 210 119 3 1 208 239 446 4.24e-53 176
MS.gene59968.t1 AT3G50270 39.906 213 122 3 1 208 237 448 4.76e-48 163
MS.gene59968.t1 AT5G67160 43.062 209 112 5 1 208 230 432 1.03e-47 162
MS.gene59968.t1 AT3G50280 39.524 210 121 3 1 205 102 310 6.41e-46 154
MS.gene59968.t1 AT3G50300 43.590 195 104 3 1 190 232 425 2.79e-45 155
MS.gene59968.t1 AT3G50280 39.524 210 121 3 1 205 232 440 1.04e-44 154
MS.gene59968.t1 AT5G07860 40.191 209 120 4 1 207 243 448 2.80e-42 148
MS.gene59968.t1 AT5G42830 38.095 210 127 3 1 208 241 449 4.13e-40 142
MS.gene59968.t1 AT5G01210 33.469 245 125 4 1 208 226 469 5.07e-39 140
MS.gene59968.t1 AT5G38130 40.206 194 110 3 1 189 250 442 1.19e-37 136
MS.gene59968.t1 AT5G07870 38.679 212 123 4 1 208 253 461 2.79e-37 135
MS.gene59968.t1 AT3G50290 46.250 160 80 3 1 155 111 269 7.97e-36 127
MS.gene59968.t1 AT5G07850 38.532 218 116 7 1 208 244 453 9.03e-36 130
MS.gene59968.t1 AT2G39980 37.838 185 113 2 25 208 297 480 1.80e-34 127
MS.gene59968.t1 AT5G23940 31.731 208 137 3 1 204 242 448 1.68e-29 114
MS.gene59968.t1 AT5G48930 25.628 199 143 3 1 195 227 424 2.23e-16 77.0
MS.gene59968.t1 AT2G19070 28.302 212 135 5 1 197 234 443 4.82e-16 76.3
MS.gene59968.t1 AT3G29590 23.383 201 151 2 2 199 242 442 9.77e-13 66.6
MS.gene59968.t1 AT5G61160 26.633 199 128 6 5 195 250 438 1.50e-12 66.2
MS.gene59968.t1 AT1G28680 28.571 161 107 4 2 160 235 389 1.73e-11 62.8
MS.gene59968.t1 AT3G29635 24.873 197 132 5 2 189 249 438 9.73e-11 60.8

Find 42 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCCTGGTTTAATGTTTA+TGG 0.116946 8.4:-57711346 MS.gene59968:CDS
GGAAATATTTGTGTGTTTAA+AGG 0.251922 8.4:-57711262 MS.gene59968:CDS
TGGTAATGATCCTGAGTTTA+TGG 0.261735 8.4:-57711179 MS.gene59968:CDS
GGGAGTGAAAATAATGAAAA+TGG 0.264658 8.4:-57711283 MS.gene59968:CDS
CCATTGAAAGAGGATTATTT+TGG 0.285934 8.4:-57711574 MS.gene59968:CDS
AATGTTTATGGTAATGATTT+TGG 0.340175 8.4:-57711334 MS.gene59968:CDS
GTGCATTATGTGGTGGTTAT+AGG 0.349094 8.4:-57711619 MS.gene59968:CDS
AAAATTCAAAGCCAACTTAG+AGG 0.353981 8.4:-57711734 MS.gene59968:CDS
CCAAAATAATCCTCTTTCAA+TGG 0.374389 8.4:+57711574 None:intergenic
CAATTGCCTCCAAAGTTTCA+TGG 0.386784 8.4:+57711200 None:intergenic
TGGACTTGGTAAAGGTGCTT+TGG 0.409489 8.4:-57711494 MS.gene59968:CDS
CATGAAACTTTGGAGGCAAT+TGG 0.418429 8.4:-57711199 MS.gene59968:CDS
AGGTGTAGAAGATGGTAGTA+TGG 0.424099 8.4:-57711242 MS.gene59968:CDS
TTTGGTTGGGGAAAACCTGT+GGG 0.472290 8.4:-57711316 MS.gene59968:CDS
CTTTGTACTAGCCTCTAAGT+TGG 0.485280 8.4:+57711723 None:intergenic
GTTGATTCCTCCATTGAAAG+AGG 0.486725 8.4:-57711584 MS.gene59968:CDS
CATAATGCACTTCTTCTTGA+GGG 0.502724 8.4:+57711632 None:intergenic
CAAGACAATGGACTTGGTAA+AGG 0.507284 8.4:-57711502 MS.gene59968:CDS
GCACTTCTTCTTGAGGGTCA+AGG 0.528530 8.4:+57711638 None:intergenic
TTCAATGGAGGAATCAACCT+TGG 0.531124 8.4:+57711589 None:intergenic
AATTGCCTCCAAAGTTTCAT+GGG 0.532415 8.4:+57711201 None:intergenic
GTGTTTGTCCCATGAAACTT+TGG 0.534872 8.4:-57711209 MS.gene59968:CDS
GTACAATAGTAAAACATTAG+TGG 0.537819 8.4:-57711377 MS.gene59968:CDS
TACAATAGTAAAACATTAGT+GGG 0.542055 8.4:-57711376 MS.gene59968:CDS
TCCGTTGTTAGCATGAAAGT+TGG 0.545219 8.4:-57711535 MS.gene59968:CDS
ACATAATGCACTTCTTCTTG+AGG 0.564314 8.4:+57711631 None:intergenic
TTGGTTGGGGAAAACCTGTG+GGG 0.567130 8.4:-57711315 MS.gene59968:CDS
TGGTTGGGGAAAACCTGTGG+GGG 0.578502 8.4:-57711314 MS.gene59968:CDS
TTTGTCCCATGAAACTTTGG+AGG 0.591981 8.4:-57711206 MS.gene59968:CDS
AAATAATCCTCTTTCAATGG+AGG 0.592205 8.4:+57711577 None:intergenic
GTGTTTAAAGGTGTAGAAGA+TGG 0.594759 8.4:-57711250 MS.gene59968:CDS
TTGTTGCAAGACAATGGACT+TGG 0.601437 8.4:-57711508 MS.gene59968:CDS
ATACCAAGAGTAATAAAACT+AGG 0.607573 8.4:+57711400 None:intergenic
GGTGATTTGTTGCAAGACAA+TGG 0.609643 8.4:-57711514 MS.gene59968:CDS
TGGTTATAGGCGTTAGACCA+AGG 0.625694 8.4:-57711606 MS.gene59968:CDS
TCAAGAAGAAGTGCATTATG+TGG 0.629418 8.4:-57711629 MS.gene59968:CDS
GTCACAACATCCATAAACTC+AGG 0.634083 8.4:+57711169 None:intergenic
AGAAGAAGTGCATTATGTGG+TGG 0.641210 8.4:-57711626 MS.gene59968:CDS
TAGTGGGTAGTAGTTCACCC+TGG 0.662355 8.4:-57711360 MS.gene59968:CDS
ATTACCATAAACATTAAACC+AGG 0.672703 8.4:+57711342 None:intergenic
ACCAACTTTCATGCTAACAA+CGG 0.674514 8.4:+57711534 None:intergenic
TTACCATAAACATTAAACCA+GGG 0.701842 8.4:+57711343 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAATAGTAAAACATTAGT+GGG - chr8.4:57711559-57711578 MS.gene59968:CDS 20.0%
!!! AATGTTTATGGTAATGATTT+TGG - chr8.4:57711601-57711620 MS.gene59968:CDS 20.0%
! ATACCAAGAGTAATAAAACT+AGG + chr8.4:57711538-57711557 None:intergenic 25.0%
! ATTACCATAAACATTAAACC+AGG + chr8.4:57711596-57711615 None:intergenic 25.0%
! GGAAATATTTGTGTGTTTAA+AGG - chr8.4:57711673-57711692 MS.gene59968:CDS 25.0%
! GTACAATAGTAAAACATTAG+TGG - chr8.4:57711558-57711577 MS.gene59968:CDS 25.0%
! TTACCATAAACATTAAACCA+GGG + chr8.4:57711595-57711614 None:intergenic 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG - chr8.4:57711606-57711625 MS.gene59968:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG - chr8.4:57711605-57711624 MS.gene59968:CDS 25.0%
AAAATTCAAAGCCAACTTAG+AGG - chr8.4:57711201-57711220 MS.gene59968:CDS 30.0%
AAATAATCCTCTTTCAATGG+AGG + chr8.4:57711361-57711380 None:intergenic 30.0%
CCAAAATAATCCTCTTTCAA+TGG + chr8.4:57711364-57711383 None:intergenic 30.0%
GGGAGTGAAAATAATGAAAA+TGG - chr8.4:57711652-57711671 MS.gene59968:CDS 30.0%
! AAGCACTTTTCACACATATT+TGG - chr8.4:57711251-57711270 MS.gene59968:CDS 30.0%
! CCATTGAAAGAGGATTATTT+TGG - chr8.4:57711361-57711380 MS.gene59968:CDS 30.0%
!!! ACACCTAGTTTTATTACTCT+TGG - chr8.4:57711532-57711551 MS.gene59968:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG - chr8.4:57711607-57711626 MS.gene59968:CDS 30.0%
AATTGCCTCCAAAGTTTCAT+GGG + chr8.4:57711737-57711756 None:intergenic 35.0%
ACATAATGCACTTCTTCTTG+AGG + chr8.4:57711307-57711326 None:intergenic 35.0%
ACCAACTTTCATGCTAACAA+CGG + chr8.4:57711404-57711423 None:intergenic 35.0%
CATAATGCACTTCTTCTTGA+GGG + chr8.4:57711306-57711325 None:intergenic 35.0%
TCACCCTGGTTTAATGTTTA+TGG - chr8.4:57711589-57711608 MS.gene59968:CDS 35.0%
TGAAAAATGACTACGAGAGT+TGG - chr8.4:57711503-57711522 MS.gene59968:CDS 35.0%
! GTGTTTAAAGGTGTAGAAGA+TGG - chr8.4:57711685-57711704 MS.gene59968:CDS 35.0%
! TCAAGAAGAAGTGCATTATG+TGG - chr8.4:57711306-57711325 MS.gene59968:CDS 35.0%
! TGGTAATGATCCTGAGTTTA+TGG - chr8.4:57711756-57711775 MS.gene59968:CDS 35.0%
AGGTGTAGAAGATGGTAGTA+TGG - chr8.4:57711693-57711712 MS.gene59968:CDS 40.0%
CAATTGCCTCCAAAGTTTCA+TGG + chr8.4:57711738-57711757 None:intergenic 40.0%
GTCACAACATCCATAAACTC+AGG + chr8.4:57711769-57711788 None:intergenic 40.0%
GTGCATTATGTGGTGGTTAT+AGG - chr8.4:57711316-57711335 MS.gene59968:CDS 40.0%
GTGTTTGTCCCATGAAACTT+TGG - chr8.4:57711726-57711745 MS.gene59968:CDS 40.0%
TCCGTTGTTAGCATGAAAGT+TGG - chr8.4:57711400-57711419 MS.gene59968:CDS 40.0%
TTCAATGGAGGAATCAACCT+TGG + chr8.4:57711349-57711368 None:intergenic 40.0%
TTTGTCCCATGAAACTTTGG+AGG - chr8.4:57711729-57711748 MS.gene59968:CDS 40.0%
! AGAAGAAGTGCATTATGTGG+TGG - chr8.4:57711309-57711328 MS.gene59968:CDS 40.0%
! CAAGACAATGGACTTGGTAA+AGG - chr8.4:57711433-57711452 MS.gene59968:CDS 40.0%
! CATGAAACTTTGGAGGCAAT+TGG - chr8.4:57711736-57711755 MS.gene59968:CDS 40.0%
! CTTTGTACTAGCCTCTAAGT+TGG + chr8.4:57711215-57711234 None:intergenic 40.0%
! GTTGATTCCTCCATTGAAAG+AGG - chr8.4:57711351-57711370 MS.gene59968:CDS 40.0%
! TTCTTGAGGGTCAAGGTTTT+TGG + chr8.4:57711293-57711312 None:intergenic 40.0%
! TTGTTGCAAGACAATGGACT+TGG - chr8.4:57711427-57711446 MS.gene59968:CDS 40.0%
!! GGTGATTTGTTGCAAGACAA+TGG - chr8.4:57711421-57711440 MS.gene59968:CDS 40.0%
TGGTTATAGGCGTTAGACCA+AGG - chr8.4:57711329-57711348 MS.gene59968:CDS 45.0%
TTTGGTTGGGGAAAACCTGT+GGG - chr8.4:57711619-57711638 MS.gene59968:CDS 45.0%
!! TGGACTTGGTAAAGGTGCTT+TGG - chr8.4:57711441-57711460 MS.gene59968:CDS 45.0%
!! TTTTGGTTGGGGAAAACCTG+TGG - chr8.4:57711618-57711637 MS.gene59968:CDS 45.0%
GCACTTCTTCTTGAGGGTCA+AGG + chr8.4:57711300-57711319 None:intergenic 50.0%
TTGGTTGGGGAAAACCTGTG+GGG - chr8.4:57711620-57711639 MS.gene59968:CDS 50.0%
! TAGTGGGTAGTAGTTCACCC+TGG - chr8.4:57711575-57711594 MS.gene59968:CDS 50.0%
AAACCTGTGGGGGTTCGAAC+TGG - chr8.4:57711631-57711650 MS.gene59968:CDS 55.0%
AACCTGTGGGGGTTCGAACT+GGG - chr8.4:57711632-57711651 MS.gene59968:CDS 55.0%
TGGTTGGGGAAAACCTGTGG+GGG - chr8.4:57711621-57711640 MS.gene59968:CDS 55.0%
CTCCCAGTTCGAACCCCCAC+AGG + chr8.4:57711637-57711656 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 57711166 57711791 57711166 ID=MS.gene59968
chr8.4 mRNA 57711166 57711791 57711166 ID=MS.gene59968.t1;Parent=MS.gene59968
chr8.4 exon 57711166 57711791 57711166 ID=MS.gene59968.t1.exon1;Parent=MS.gene59968.t1
chr8.4 CDS 57711166 57711791 57711166 ID=cds.MS.gene59968.t1;Parent=MS.gene59968.t1
Gene Sequence

>MS.gene59968

ATGTTTCATTTCACAAAAGAGAATATTGCAAAACTAAAATTCAAAGCCAACTTAGAGGCTAGTACAAAGAACATATCTTCTTTGCAAGCACTTTTCACACATATTTGGCGATCTATTATACGTTCCAAAAACCTTGACCCTCAAGAAGAAGTGCATTATGTGGTGGTTATAGGCGTTAGACCAAGGTTGATTCCTCCATTGAAAGAGGATTATTTTGGTAATGCTATGATAGACTCCGTTGTTAGCATGAAAGTTGGTGATTTGTTGCAAGACAATGGACTTGGTAAAGGTGCTTTGGAGATGAACAAGATGATTGCTTTGCAATCTGATGAGATGTTGAAAAATGACTACGAGAGTTGGTTGAAAACACCTAGTTTTATTACTCTTGGTATGTACAATAGTAAAACATTAGTGGGTAGTAGTTCACCCTGGTTTAATGTTTATGGTAATGATTTTGGTTGGGGAAAACCTGTGGGGGTTCGAACTGGGAGTGAAAATAATGAAAATGGAAATATTTGTGTGTTTAAAGGTGTAGAAGATGGTAGTATGGATCTTGAAGTGTGTTTGTCCCATGAAACTTTGGAGGCAATTGGTAATGATCCTGAGTTTATGGATGTTGTGACCAA

Protein sequence

>MS.gene59968.t1

MFHFTKENIAKLKFKANLEASTKNISSLQALFTHIWRSIIRSKNLDPQEEVHYVVVIGVRPRLIPPLKEDYFGNAMIDSVVSMKVGDLLQDNGLGKGALEMNKMIALQSDEMLKNDYESWLKTPSFITLGMYNSKTLVGSSSPWFNVYGNDFGWGKPVGVRTGSENNENGNICVFKGVEDGSMDLEVCLSHETLEAIGNDPEFMDVVT