Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60838.t1 | XP_013469340.1 | 69.1 | 275 | 81 | 2 | 1 | 272 | 1 | 274 | 1.50E-98 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60838.t1 | Q41160 | 53.8 | 275 | 118 | 4 | 5 | 273 | 1 | 272 | 5.3e-73 | 275.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60838.t1 | A0A072VPS3 | 69.1 | 275 | 81 | 2 | 1 | 272 | 1 | 274 | 1.1e-98 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene60838 | 0.834898 | 2.30E-56 | -1.69E-46 |
| MS.gene052223 | MS.gene60838 | 0.82141 | 4.10E-53 | -1.69E-46 |
| MS.gene052702 | MS.gene60838 | 0.838391 | 2.96E-57 | -1.69E-46 |
| MS.gene052706 | MS.gene60838 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene054290 | MS.gene60838 | 0.809101 | 2.24E-50 | -1.69E-46 |
| MS.gene056590 | MS.gene60838 | 0.806686 | 7.29E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene60838 | 0.807139 | 5.85E-50 | -1.69E-46 |
| MS.gene056688 | MS.gene60838 | 0.801594 | 8.36E-49 | -1.69E-46 |
| MS.gene056690 | MS.gene60838 | 0.814837 | 1.26E-51 | -1.69E-46 |
| MS.gene056691 | MS.gene60838 | 0.809714 | 1.65E-50 | -1.69E-46 |
| MS.gene056697 | MS.gene60838 | 0.833219 | 6.05E-56 | -1.69E-46 |
| MS.gene058381 | MS.gene60838 | 0.808802 | 2.59E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene60838 | 0.823211 | 1.57E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60838.t1 | MTR_1g090973 | 69.091 | 275 | 81 | 2 | 1 | 272 | 1 | 274 | 1.99e-130 | 371 |
| MS.gene60838.t1 | MTR_1g090960 | 61.566 | 281 | 62 | 4 | 1 | 272 | 1 | 244 | 2.08e-108 | 314 |
| MS.gene60838.t1 | MTR_3g054170 | 56.432 | 241 | 102 | 2 | 21 | 261 | 17 | 254 | 9.63e-92 | 272 |
| MS.gene60838.t1 | MTR_0163s0020 | 50.357 | 280 | 130 | 3 | 1 | 273 | 2 | 279 | 8.46e-90 | 268 |
| MS.gene60838.t1 | MTR_0684s0020 | 50.357 | 280 | 130 | 3 | 1 | 273 | 1 | 278 | 8.56e-90 | 268 |
| MS.gene60838.t1 | MTR_3g047140 | 52.593 | 270 | 126 | 2 | 1 | 269 | 1 | 269 | 5.28e-89 | 266 |
| MS.gene60838.t1 | MTR_3g054200 | 53.942 | 241 | 108 | 2 | 21 | 261 | 17 | 254 | 7.78e-87 | 259 |
| MS.gene60838.t1 | MTR_3g054180 | 53.086 | 243 | 109 | 3 | 21 | 261 | 21 | 260 | 1.26e-83 | 251 |
| MS.gene60838.t1 | MTR_1g090943 | 49.603 | 252 | 123 | 3 | 14 | 264 | 15 | 263 | 9.68e-71 | 219 |
| MS.gene60838.t1 | MTR_3g027280 | 47.826 | 276 | 126 | 11 | 1 | 264 | 1 | 270 | 2.07e-66 | 208 |
| MS.gene60838.t1 | MTR_1g090957 | 44.056 | 286 | 132 | 11 | 1 | 275 | 1 | 269 | 7.30e-59 | 189 |
| MS.gene60838.t1 | MTR_1g090937 | 43.066 | 274 | 137 | 10 | 1 | 265 | 1 | 264 | 1.75e-57 | 185 |
| MS.gene60838.t1 | MTR_1g090930 | 41.877 | 277 | 140 | 10 | 1 | 265 | 1 | 268 | 5.20e-52 | 171 |
| MS.gene60838.t1 | MTR_8g068040 | 40.755 | 265 | 129 | 11 | 14 | 262 | 10 | 262 | 1.06e-47 | 160 |
| MS.gene60838.t1 | MTR_8g068030 | 39.630 | 270 | 129 | 12 | 14 | 262 | 10 | 266 | 2.30e-46 | 156 |
| MS.gene60838.t1 | MTR_5g031160 | 38.194 | 288 | 138 | 13 | 6 | 261 | 8 | 287 | 2.86e-44 | 151 |
| MS.gene60838.t1 | MTR_5g031120 | 40.562 | 249 | 119 | 11 | 35 | 261 | 38 | 279 | 1.34e-42 | 147 |
| MS.gene60838.t1 | MTR_5g031100 | 39.919 | 248 | 121 | 10 | 35 | 261 | 38 | 278 | 2.63e-42 | 146 |
| MS.gene60838.t1 | MTR_5g031140 | 36.585 | 287 | 144 | 13 | 5 | 261 | 8 | 286 | 5.49e-41 | 143 |
| MS.gene60838.t1 | MTR_4g056320 | 36.567 | 268 | 126 | 7 | 2 | 265 | 6 | 233 | 1.34e-40 | 141 |
| MS.gene60838.t1 | MTR_8g067980 | 39.662 | 237 | 126 | 8 | 36 | 259 | 25 | 257 | 1.55e-40 | 141 |
| MS.gene60838.t1 | MTR_8g023510 | 38.806 | 268 | 148 | 10 | 1 | 261 | 1 | 259 | 3.24e-39 | 138 |
| MS.gene60838.t1 | MTR_8g067990 | 37.553 | 237 | 131 | 8 | 36 | 259 | 25 | 257 | 1.50e-38 | 136 |
| MS.gene60838.t1 | MTR_8g068050 | 36.765 | 272 | 151 | 10 | 11 | 269 | 18 | 281 | 3.31e-38 | 142 |
| MS.gene60838.t1 | MTR_4g023810 | 38.983 | 236 | 132 | 8 | 30 | 261 | 27 | 254 | 1.76e-34 | 125 |
| MS.gene60838.t1 | MTR_1g014240 | 37.860 | 243 | 131 | 9 | 38 | 264 | 40 | 278 | 9.25e-33 | 127 |
| MS.gene60838.t1 | MTR_4g093080 | 35.926 | 270 | 143 | 12 | 17 | 268 | 9 | 266 | 9.56e-33 | 127 |
| MS.gene60838.t1 | MTR_8g067960 | 39.732 | 224 | 119 | 9 | 49 | 261 | 23 | 241 | 5.80e-32 | 118 |
| MS.gene60838.t1 | MTR_4g056360 | 34.211 | 266 | 131 | 10 | 2 | 261 | 6 | 233 | 9.29e-31 | 115 |
| MS.gene60838.t1 | MTR_5g031090 | 34.553 | 246 | 135 | 11 | 37 | 261 | 35 | 275 | 1.32e-29 | 113 |
| MS.gene60838.t1 | MTR_8g067940 | 35.252 | 278 | 147 | 15 | 8 | 261 | 13 | 281 | 8.98e-29 | 111 |
| MS.gene60838.t1 | MTR_5g031030 | 32.922 | 243 | 143 | 8 | 35 | 261 | 36 | 274 | 1.03e-28 | 110 |
| MS.gene60838.t1 | MTR_8g067720 | 34.559 | 272 | 155 | 11 | 9 | 268 | 4 | 264 | 1.53e-27 | 111 |
| MS.gene60838.t1 | MTR_7g115740 | 32.157 | 255 | 152 | 9 | 23 | 262 | 13 | 261 | 2.05e-27 | 111 |
| MS.gene60838.t1 | MTR_7g116130 | 31.461 | 267 | 162 | 7 | 15 | 266 | 6 | 266 | 2.39e-27 | 111 |
| MS.gene60838.t1 | MTR_8g067690 | 36.555 | 238 | 137 | 8 | 35 | 261 | 60 | 294 | 1.05e-26 | 109 |
| MS.gene60838.t1 | MTR_4g093110 | 34.363 | 259 | 143 | 13 | 19 | 262 | 19 | 265 | 1.16e-25 | 106 |
| MS.gene60838.t1 | MTR_8g067735 | 35.391 | 243 | 140 | 9 | 35 | 264 | 43 | 281 | 1.61e-25 | 105 |
| MS.gene60838.t1 | MTR_8g067930 | 34.586 | 266 | 155 | 9 | 15 | 268 | 8 | 266 | 1.92e-25 | 105 |
| MS.gene60838.t1 | MTR_4g093040 | 33.208 | 265 | 155 | 8 | 5 | 254 | 3 | 260 | 1.06e-24 | 103 |
| MS.gene60838.t1 | MTR_4g093140 | 32.819 | 259 | 147 | 12 | 19 | 262 | 19 | 265 | 1.44e-24 | 103 |
| MS.gene60838.t1 | MTR_4g093070 | 35.354 | 198 | 114 | 6 | 77 | 262 | 72 | 267 | 1.22e-22 | 97.4 |
| MS.gene60838.t1 | MTR_8g067630 | 34.375 | 224 | 133 | 7 | 35 | 247 | 30 | 250 | 2.08e-22 | 97.1 |
| MS.gene60838.t1 | MTR_3g463370 | 29.368 | 269 | 136 | 7 | 11 | 269 | 6 | 230 | 3.42e-20 | 87.4 |
| MS.gene60838.t1 | MTR_4g093040 | 37.569 | 181 | 95 | 6 | 87 | 254 | 3 | 178 | 5.48e-20 | 89.7 |
| MS.gene60838.t1 | MTR_4g093050 | 32.773 | 238 | 142 | 11 | 36 | 257 | 27 | 262 | 2.15e-18 | 85.1 |
| MS.gene60838.t1 | MTR_5g068770 | 32.389 | 247 | 135 | 11 | 38 | 259 | 38 | 277 | 3.74e-17 | 81.6 |
| MS.gene60838.t1 | MTR_1g048360 | 29.744 | 195 | 117 | 6 | 77 | 259 | 74 | 260 | 5.10e-15 | 75.1 |
| MS.gene60838.t1 | MTR_7g115740 | 30.519 | 154 | 98 | 5 | 23 | 170 | 13 | 163 | 1.89e-14 | 73.6 |
| MS.gene60838.t1 | MTR_7g062940 | 28.571 | 266 | 149 | 11 | 22 | 260 | 7 | 258 | 3.08e-13 | 69.7 |
| MS.gene60838.t1 | MTR_5g025020 | 28.000 | 200 | 119 | 7 | 76 | 255 | 87 | 281 | 8.90e-13 | 68.6 |
| MS.gene60838.t1 | MTR_5g077100 | 31.797 | 217 | 125 | 8 | 59 | 263 | 49 | 254 | 1.27e-12 | 68.2 |
| MS.gene60838.t1 | MTR_7g062890 | 28.044 | 271 | 167 | 10 | 16 | 265 | 1 | 264 | 2.76e-11 | 63.9 |
| MS.gene60838.t1 | MTR_2g090250 | 29.688 | 256 | 150 | 11 | 41 | 272 | 29 | 278 | 3.04e-11 | 63.9 |
| MS.gene60838.t1 | MTR_7g063010 | 31.646 | 237 | 123 | 11 | 50 | 260 | 36 | 259 | 9.58e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60838.t1 | AT5G10530 | 39.837 | 246 | 121 | 13 | 35 | 262 | 19 | 255 | 2.04e-31 | 123 |
| MS.gene60838.t1 | AT5G65600 | 34.081 | 223 | 128 | 9 | 35 | 241 | 35 | 254 | 8.10e-23 | 98.2 |
| MS.gene60838.t1 | AT5G06740 | 31.559 | 263 | 138 | 12 | 22 | 258 | 11 | 257 | 7.02e-18 | 83.6 |
| MS.gene60838.t1 | AT5G06740 | 31.559 | 263 | 138 | 12 | 22 | 258 | 23 | 269 | 7.07e-18 | 83.6 |
| MS.gene60838.t1 | AT5G55830 | 30.224 | 268 | 159 | 11 | 14 | 260 | 7 | 267 | 9.30e-16 | 77.4 |
| MS.gene60838.t1 | AT5G03140 | 29.762 | 252 | 159 | 9 | 17 | 255 | 11 | 257 | 1.75e-14 | 73.6 |
| MS.gene60838.t1 | AT4G02410 | 27.757 | 263 | 151 | 11 | 31 | 266 | 22 | 272 | 2.89e-13 | 70.1 |
| MS.gene60838.t1 | AT3G53810 | 28.287 | 251 | 157 | 7 | 29 | 260 | 18 | 264 | 7.95e-13 | 68.6 |
| MS.gene60838.t1 | AT4G04960 | 30.172 | 232 | 130 | 8 | 36 | 244 | 21 | 243 | 8.40e-13 | 68.6 |
| MS.gene60838.t1 | AT4G04960 | 30.172 | 232 | 130 | 9 | 36 | 244 | 21 | 243 | 1.15e-12 | 68.2 |
| MS.gene60838.t1 | AT4G02420 | 27.820 | 266 | 162 | 11 | 17 | 260 | 6 | 263 | 4.90e-12 | 66.2 |
| MS.gene60838.t1 | AT5G01560 | 30.000 | 250 | 142 | 10 | 38 | 259 | 30 | 274 | 7.37e-12 | 65.9 |
| MS.gene60838.t1 | AT2G37710 | 26.000 | 250 | 160 | 8 | 31 | 260 | 20 | 264 | 8.55e-12 | 65.5 |
| MS.gene60838.t1 | AT4G29050 | 27.753 | 227 | 128 | 9 | 60 | 260 | 59 | 275 | 1.21e-11 | 65.1 |
| MS.gene60838.t1 | AT4G29050 | 27.753 | 227 | 128 | 9 | 60 | 260 | 45 | 261 | 1.34e-11 | 65.1 |
Find 75 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCCAACATGTGTGTATT+TGG | 0.093315 | 1.4:+72983029 | None:intergenic |
| GAAAAGCTTGCAATATTATT+TGG | 0.099570 | 1.4:+72982727 | None:intergenic |
| TTTCTTTAAAGAAAATAAAA+TGG | 0.247146 | 1.4:-72982990 | MS.gene60838:CDS |
| AAAAGCTTGCAATATTATTT+GGG | 0.253456 | 1.4:+72982728 | None:intergenic |
| ATTCAGCACCGGTGCCCATT+TGG | 0.272619 | 1.4:-72983281 | MS.gene60838:CDS |
| CCCAACAGATCCAAACTATT+TGG | 0.303319 | 1.4:-72983319 | MS.gene60838:CDS |
| ACTTCCTTGTAGGGAAATTA+TGG | 0.312613 | 1.4:+72983380 | None:intergenic |
| TTCAGCACCGGTGCCCATTT+GGG | 0.316239 | 1.4:-72983280 | MS.gene60838:CDS |
| TAAAGAAAATAAAATGGAAT+AGG | 0.318424 | 1.4:-72982984 | MS.gene60838:CDS |
| AGTTGAGTTGCTAGGAATAT+TGG | 0.321296 | 1.4:+72983140 | None:intergenic |
| TGACTAACACAACAGAAAAT+AGG | 0.328825 | 1.4:+72983496 | None:intergenic |
| ATTCCAACATGTGTGTATTT+GGG | 0.336887 | 1.4:+72983030 | None:intergenic |
| CATCAGATGTGACAAACTTT+TGG | 0.346785 | 1.4:+72983196 | None:intergenic |
| GCCCGACCAGCCAAATAGTT+TGG | 0.378898 | 1.4:+72983309 | None:intergenic |
| GCCAAATAGTTTGGATCTGT+TGG | 0.383215 | 1.4:+72983318 | None:intergenic |
| GCCAGTGGTTTCATCCCAAA+TGG | 0.385140 | 1.4:+72983266 | None:intergenic |
| AAATTGAGGCACTTCCTTGT+AGG | 0.404967 | 1.4:+72983370 | None:intergenic |
| TGGGCACCGGTGCTGAATAC+AGG | 0.408005 | 1.4:+72983286 | None:intergenic |
| AAAGAAAATAAAATGGAATA+GGG | 0.416250 | 1.4:-72982983 | MS.gene60838:CDS |
| AGCTAAACAACCTCCATTCA+TGG | 0.418791 | 1.4:-72982780 | MS.gene60838:CDS |
| CAAAAGTTTGTCACATCTGA+TGG | 0.426781 | 1.4:-72983195 | MS.gene60838:CDS |
| AATTGAGGCACTTCCTTGTA+GGG | 0.429899 | 1.4:+72983371 | None:intergenic |
| GTCGATCAAACCAAATCATA+TGG | 0.434983 | 1.4:+72982702 | None:intergenic |
| AAACCAATCCTTACGGTGTT+CGG | 0.435951 | 1.4:+72982832 | None:intergenic |
| ATGGTCTTTCAAGTCTAACT+TGG | 0.437947 | 1.4:-72982761 | MS.gene60838:CDS |
| GGACTTACTATTACTGAAGT+TGG | 0.442645 | 1.4:+72983401 | None:intergenic |
| AGTTTGATAGTTATGCCAAT+AGG | 0.444610 | 1.4:-72983059 | MS.gene60838:CDS |
| ATAGTTGAAAAGGTACCATC+TGG | 0.449708 | 1.4:+72982886 | None:intergenic |
| TCCGCATACAATCCATTTGT+TGG | 0.449749 | 1.4:-72983087 | MS.gene60838:CDS |
| GTATTTGGGATCCCACCTAT+TGG | 0.452286 | 1.4:+72983044 | None:intergenic |
| CCAAATAGTTTGGATCTGTT+GGG | 0.452907 | 1.4:+72983319 | None:intergenic |
| TCCAAACTATTTGGCTGGTC+GGG | 0.455927 | 1.4:-72983310 | MS.gene60838:CDS |
| CTTCCGAACACCGTAAGGAT+TGG | 0.469222 | 1.4:-72982835 | MS.gene60838:CDS |
| TCAAACTCAACACCAACAAA+TGG | 0.471318 | 1.4:+72983075 | None:intergenic |
| TAGACTTGAAAGACCATGAA+TGG | 0.487117 | 1.4:+72982767 | None:intergenic |
| GACTTGAGCAATAGTTGAAA+AGG | 0.492838 | 1.4:+72982876 | None:intergenic |
| TGTCTTAGAGAGATTGTCAT+AGG | 0.497716 | 1.4:+72982930 | None:intergenic |
| ATAAAATGGAATAGGGTGAA+TGG | 0.502888 | 1.4:-72982976 | MS.gene60838:CDS |
| AACTCAACTGGTGGAAACCT+TGG | 0.508020 | 1.4:-72983126 | MS.gene60838:CDS |
| GGCTATATCCAAGATTCCAT+TGG | 0.512019 | 1.4:+72983344 | None:intergenic |
| GATTCCATTGGATAAAATTG+AGG | 0.512081 | 1.4:+72983356 | None:intergenic |
| ATCCAAACTATTTGGCTGGT+CGG | 0.513232 | 1.4:-72983311 | MS.gene60838:CDS |
| CCAGTGGTTTCATCCCAAAT+GGG | 0.521697 | 1.4:+72983267 | None:intergenic |
| TTTCCACCAGTTGAGTTGCT+AGG | 0.525423 | 1.4:+72983132 | None:intergenic |
| ATGGAGGTTGTTTAGCTGAC+CGG | 0.532538 | 1.4:+72982786 | None:intergenic |
| AATATTCCTAGCAACTCAAC+TGG | 0.536663 | 1.4:-72983138 | MS.gene60838:CDS |
| GGAATATTGGCTCGATCTTG+TGG | 0.539230 | 1.4:+72983153 | None:intergenic |
| TCGGGCCCTGTATTCAGCAC+CGG | 0.539297 | 1.4:-72983292 | MS.gene60838:CDS |
| ACAGATCCAAACTATTTGGC+TGG | 0.541475 | 1.4:-72983315 | MS.gene60838:CDS |
| GGGCACCGGTGCTGAATACA+GGG | 0.542627 | 1.4:+72983287 | None:intergenic |
| TTGATAGTTATGCCAATAGG+TGG | 0.543485 | 1.4:-72983056 | MS.gene60838:CDS |
| CCCATTTGGGATGAAACCAC+TGG | 0.547396 | 1.4:-72983267 | MS.gene60838:CDS |
| ATAGTTTGGATCTGTTGGGT+TGG | 0.547533 | 1.4:+72983323 | None:intergenic |
| GAATAGGGTGAATGGCGCCT+TGG | 0.550930 | 1.4:-72982968 | MS.gene60838:CDS |
| GCTTGCAATATTATTTGGGG+TGG | 0.556881 | 1.4:+72982732 | None:intergenic |
| AAAGCTTGCAATATTATTTG+GGG | 0.560702 | 1.4:+72982729 | None:intergenic |
| CAGAGCTTCCGAACACCGTA+AGG | 0.561999 | 1.4:-72982840 | MS.gene60838:CDS |
| GCTGCCACATCAAGAGGTGC+CGG | 0.566269 | 1.4:-72982805 | MS.gene60838:CDS |
| GTCATGCACTCTGAATGAAA+AGG | 0.571753 | 1.4:+72983221 | None:intergenic |
| AAGTCCATAATTTCCCTACA+AGG | 0.572121 | 1.4:-72983384 | MS.gene60838:CDS |
| GATCCCAAATACACACATGT+TGG | 0.576936 | 1.4:-72983033 | MS.gene60838:CDS |
| ATTCCTAGCAACTCAACTGG+TGG | 0.582588 | 1.4:-72983135 | MS.gene60838:CDS |
| ACTGAAGTTGGTGATGTCGA+AGG | 0.592142 | 1.4:+72983413 | None:intergenic |
| GAAGCTCGCCACGTTGCCAG+TGG | 0.595816 | 1.4:+72983251 | None:intergenic |
| GTTTCATCCCAAATGGGCAC+CGG | 0.596050 | 1.4:+72983273 | None:intergenic |
| TGATAGTTATGCCAATAGGT+GGG | 0.619156 | 1.4:-72983055 | MS.gene60838:CDS |
| ATTTGTTGGAAAGAAAGCCA+TGG | 0.619705 | 1.4:+72983524 | None:intergenic |
| GTGGTTGTATCTTACCCAGA+TGG | 0.625936 | 1.4:-72982901 | MS.gene60838:CDS |
| CTGACCGGCACCTCTTGATG+TGG | 0.630323 | 1.4:+72982801 | None:intergenic |
| TAGTTGAAAAGGTACCATCT+GGG | 0.650948 | 1.4:+72982887 | None:intergenic |
| TCTCTCTAAGACATTGAATG+TGG | 0.660926 | 1.4:-72982920 | MS.gene60838:CDS |
| ACCAACAAATGGATTGTATG+CGG | 0.667112 | 1.4:+72983086 | None:intergenic |
| ACTTGAAAGACCATGAATGG+AGG | 0.678079 | 1.4:+72982770 | None:intergenic |
| GGAGATATTAACATCGACCA+AGG | 0.682054 | 1.4:+72982951 | None:intergenic |
| GGATGAAACCACTGGCAACG+TGG | 0.707919 | 1.4:-72983259 | MS.gene60838:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCTTTAAAGAAAATAAAA+TGG | - | chr1.4:72983249-72983268 | MS.gene60838:CDS | 10.0% |
| !! | AAAGAAAATAAAATGGAATA+GGG | - | chr1.4:72983256-72983275 | MS.gene60838:CDS | 15.0% |
| !! | TAAAGAAAATAAAATGGAAT+AGG | - | chr1.4:72983255-72983274 | MS.gene60838:CDS | 15.0% |
| !! | AAAAGCTTGCAATATTATTT+GGG | + | chr1.4:72983514-72983533 | None:intergenic | 20.0% |
| ! | AAAGCTTGCAATATTATTTG+GGG | + | chr1.4:72983513-72983532 | None:intergenic | 25.0% |
| ! | GAAAAGCTTGCAATATTATT+TGG | + | chr1.4:72983515-72983534 | None:intergenic | 25.0% |
| !! | AATTTTATCCAATGGAATCT+TGG | - | chr1.4:72982887-72982906 | MS.gene60838:CDS | 25.0% |
| !! | AGTTTTCATTAACAATTCCA+AGG | + | chr1.4:72983133-72983152 | None:intergenic | 25.0% |
| !!! | GAAAATAGGTTTTGATTTGT+TGG | + | chr1.4:72982732-72982751 | None:intergenic | 25.0% |
| AATTCCAACATGTGTGTATT+TGG | + | chr1.4:72983213-72983232 | None:intergenic | 30.0% | |
| ATAAAATGGAATAGGGTGAA+TGG | - | chr1.4:72983263-72983282 | MS.gene60838:CDS | 30.0% | |
| ATTCCAACATGTGTGTATTT+GGG | + | chr1.4:72983212-72983231 | None:intergenic | 30.0% | |
| GATTCCATTGGATAAAATTG+AGG | + | chr1.4:72982886-72982905 | None:intergenic | 30.0% | |
| TGACTAACACAACAGAAAAT+AGG | + | chr1.4:72982746-72982765 | None:intergenic | 30.0% | |
| ! | AGTTTGATAGTTATGCCAAT+AGG | - | chr1.4:72983180-72983199 | MS.gene60838:CDS | 30.0% |
| AAGTCCATAATTTCCCTACA+AGG | - | chr1.4:72982855-72982874 | MS.gene60838:CDS | 35.0% | |
| AATATTCCTAGCAACTCAAC+TGG | - | chr1.4:72983101-72983120 | MS.gene60838:CDS | 35.0% | |
| ACTTCCTTGTAGGGAAATTA+TGG | + | chr1.4:72982862-72982881 | None:intergenic | 35.0% | |
| AGCAGAAAAACCAATCCTTA+CGG | + | chr1.4:72983417-72983436 | None:intergenic | 35.0% | |
| CAAAAGTTTGTCACATCTGA+TGG | - | chr1.4:72983044-72983063 | MS.gene60838:CDS | 35.0% | |
| CCAAATAGTTTGGATCTGTT+GGG | + | chr1.4:72982923-72982942 | None:intergenic | 35.0% | |
| GACTTGAGCAATAGTTGAAA+AGG | + | chr1.4:72983366-72983385 | None:intergenic | 35.0% | |
| GGACTTACTATTACTGAAGT+TGG | + | chr1.4:72982841-72982860 | None:intergenic | 35.0% | |
| TAGACTTGAAAGACCATGAA+TGG | + | chr1.4:72983475-72983494 | None:intergenic | 35.0% | |
| TCAAACTCAACACCAACAAA+TGG | + | chr1.4:72983167-72983186 | None:intergenic | 35.0% | |
| TGATAGTTATGCCAATAGGT+GGG | - | chr1.4:72983184-72983203 | MS.gene60838:CDS | 35.0% | |
| TGTCTTAGAGAGATTGTCAT+AGG | + | chr1.4:72983312-72983331 | None:intergenic | 35.0% | |
| ! | AGTGCCTCAATTTTATCCAA+TGG | - | chr1.4:72982879-72982898 | MS.gene60838:CDS | 35.0% |
| ! | AGTTGAGTTGCTAGGAATAT+TGG | + | chr1.4:72983102-72983121 | None:intergenic | 35.0% |
| ! | ATGGTCTTTCAAGTCTAACT+TGG | - | chr1.4:72983478-72983497 | MS.gene60838:CDS | 35.0% |
| ! | CATCAGATGTGACAAACTTT+TGG | + | chr1.4:72983046-72983065 | None:intergenic | 35.0% |
| ! | TTGATAGTTATGCCAATAGG+TGG | - | chr1.4:72983183-72983202 | MS.gene60838:CDS | 35.0% |
| !! | ACCAACAAATGGATTGTATG+CGG | + | chr1.4:72983156-72983175 | None:intergenic | 35.0% |
| !! | ATAGTTGAAAAGGTACCATC+TGG | + | chr1.4:72983356-72983375 | None:intergenic | 35.0% |
| !! | TAGTTGAAAAGGTACCATCT+GGG | + | chr1.4:72983355-72983374 | None:intergenic | 35.0% |
| !! | TCTCTCTAAGACATTGAATG+TGG | - | chr1.4:72983319-72983338 | MS.gene60838:CDS | 35.0% |
| !!! | GAAGGAAACTGTTTTTTGTG+AGG | + | chr1.4:72982811-72982830 | None:intergenic | 35.0% |
| AAACCAATCCTTACGGTGTT+CGG | + | chr1.4:72983410-72983429 | None:intergenic | 40.0% | |
| ACAGATCCAAACTATTTGGC+TGG | - | chr1.4:72982924-72982943 | MS.gene60838:CDS | 40.0% | |
| ACTTGAAAGACCATGAATGG+AGG | + | chr1.4:72983472-72983491 | None:intergenic | 40.0% | |
| AGCTAAACAACCTCCATTCA+TGG | - | chr1.4:72983459-72983478 | MS.gene60838:CDS | 40.0% | |
| ATAGTTTGGATCTGTTGGGT+TGG | + | chr1.4:72982919-72982938 | None:intergenic | 40.0% | |
| ATCCAAACTATTTGGCTGGT+CGG | - | chr1.4:72982928-72982947 | MS.gene60838:CDS | 40.0% | |
| CCCAACAGATCCAAACTATT+TGG | - | chr1.4:72982920-72982939 | MS.gene60838:CDS | 40.0% | |
| GATCCCAAATACACACATGT+TGG | - | chr1.4:72983206-72983225 | MS.gene60838:CDS | 40.0% | |
| GCCAAATAGTTTGGATCTGT+TGG | + | chr1.4:72982924-72982943 | None:intergenic | 40.0% | |
| GCTTGCAATATTATTTGGGG+TGG | + | chr1.4:72983510-72983529 | None:intergenic | 40.0% | |
| GGAGATATTAACATCGACCA+AGG | + | chr1.4:72983291-72983310 | None:intergenic | 40.0% | |
| GGCTATATCCAAGATTCCAT+TGG | + | chr1.4:72982898-72982917 | None:intergenic | 40.0% | |
| GTCATGCACTCTGAATGAAA+AGG | + | chr1.4:72983021-72983040 | None:intergenic | 40.0% | |
| TCCGCATACAATCCATTTGT+TGG | - | chr1.4:72983152-72983171 | MS.gene60838:CDS | 40.0% | |
| ! | AAATTGAGGCACTTCCTTGT+AGG | + | chr1.4:72982872-72982891 | None:intergenic | 40.0% |
| ! | AATTGAGGCACTTCCTTGTA+GGG | + | chr1.4:72982871-72982890 | None:intergenic | 40.0% |
| AACTCAACTGGTGGAAACCT+TGG | - | chr1.4:72983113-72983132 | MS.gene60838:CDS | 45.0% | |
| ATGGAGGTTGTTTAGCTGAC+CGG | + | chr1.4:72983456-72983475 | None:intergenic | 45.0% | |
| ATTCCTAGCAACTCAACTGG+TGG | - | chr1.4:72983104-72983123 | MS.gene60838:CDS | 45.0% | |
| CCAGTGGTTTCATCCCAAAT+GGG | + | chr1.4:72982975-72982994 | None:intergenic | 45.0% | |
| GTGGTTGTATCTTACCCAGA+TGG | - | chr1.4:72983338-72983357 | MS.gene60838:CDS | 45.0% | |
| ! | GTATTTGGGATCCCACCTAT+TGG | + | chr1.4:72983198-72983217 | None:intergenic | 45.0% |
| ! | TCCAAACTATTTGGCTGGTC+GGG | - | chr1.4:72982929-72982948 | MS.gene60838:CDS | 45.0% |
| ! | TTTCCACCAGTTGAGTTGCT+AGG | + | chr1.4:72983110-72983129 | None:intergenic | 45.0% |
| ! | TTTTCTGCTGCCACATCAAG+AGG | - | chr1.4:72983428-72983447 | MS.gene60838:CDS | 45.0% |
| !! | ACTGAAGTTGGTGATGTCGA+AGG | + | chr1.4:72982829-72982848 | None:intergenic | 45.0% |
| !! | GGAATATTGGCTCGATCTTG+TGG | + | chr1.4:72983089-72983108 | None:intergenic | 45.0% |
| CTTCCGAACACCGTAAGGAT+TGG | - | chr1.4:72983404-72983423 | MS.gene60838:CDS | 50.0% | |
| GCCAGTGGTTTCATCCCAAA+TGG | + | chr1.4:72982976-72982995 | None:intergenic | 50.0% | |
| GTTTCATCCCAAATGGGCAC+CGG | + | chr1.4:72982969-72982988 | None:intergenic | 50.0% | |
| ! | CCCATTTGGGATGAAACCAC+TGG | - | chr1.4:72982972-72982991 | MS.gene60838:CDS | 50.0% |
| CAGAGCTTCCGAACACCGTA+AGG | - | chr1.4:72983399-72983418 | MS.gene60838:CDS | 55.0% | |
| GAATAGGGTGAATGGCGCCT+TGG | - | chr1.4:72983271-72983290 | MS.gene60838:CDS | 55.0% | |
| GCCCGACCAGCCAAATAGTT+TGG | + | chr1.4:72982933-72982952 | None:intergenic | 55.0% | |
| GGATGAAACCACTGGCAACG+TGG | - | chr1.4:72982980-72982999 | MS.gene60838:CDS | 55.0% | |
| !! | ATTCAGCACCGGTGCCCATT+TGG | - | chr1.4:72982958-72982977 | MS.gene60838:CDS | 55.0% |
| !! | TTCAGCACCGGTGCCCATTT+GGG | - | chr1.4:72982959-72982978 | MS.gene60838:CDS | 55.0% |
| CTGACCGGCACCTCTTGATG+TGG | + | chr1.4:72983441-72983460 | None:intergenic | 60.0% | |
| TCGGGCCCTGTATTCAGCAC+CGG | - | chr1.4:72982947-72982966 | MS.gene60838:CDS | 60.0% | |
| ! | GCTGCCACATCAAGAGGTGC+CGG | - | chr1.4:72983434-72983453 | MS.gene60838:CDS | 60.0% |
| !! | GGGCACCGGTGCTGAATACA+GGG | + | chr1.4:72982955-72982974 | None:intergenic | 60.0% |
| !! | TGGGCACCGGTGCTGAATAC+AGG | + | chr1.4:72982956-72982975 | None:intergenic | 60.0% |
| GAAGCTCGCCACGTTGCCAG+TGG | + | chr1.4:72982991-72983010 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 72982717 | 72983544 | 72982717 | ID=MS.gene60838 |
| chr1.4 | mRNA | 72982717 | 72983544 | 72982717 | ID=MS.gene60838.t1;Parent=MS.gene60838 |
| chr1.4 | exon | 72982717 | 72983544 | 72982717 | ID=MS.gene60838.t1.exon1;Parent=MS.gene60838.t1 |
| chr1.4 | CDS | 72982717 | 72983544 | 72982717 | ID=cds.MS.gene60838.t1;Parent=MS.gene60838.t1 |
| Gene Sequence |
| Protein sequence |
